RESUMEN
OBJECTIVES: To evaluate the mRNA expression levels of cytokines interferon-γ, tumour necrosis factor-α, interleukin IL-1ß, IL-10, and the chemokine CCL2/MCP-1, CCL4, and CXCR4 in the periapical interstitial fluid from root canal infections before and after bacterial load reduction in patients undergoing haematopoietic stem cell transplantation (HSCT). MATERIALS AND METHODS: The case group was composed of 10 patients undergoing HSCT, and our control group included 10 healthy patients. Clinical samples were taken from teeth with pulp necrosis. Three paper points were placed in the RCS and maintained for 2 min for microbial evaluation before cleaning and shaping procedures. After cleaning and drying the canal, three paper points were introduced into the root canal, passing passively through the root apex (2 mm) into the periapical tissues for 1 min. Samples were collected immediately after root canal cleaning and 7 days later (restrained root canal bacterial load) to characterize gene expression using real-time PCR. RESULTS: The results showed significantly reduction in the microbial load on day 7. An increased expression level of TNF-α and IFN-γ on day 7 in control and case groups was observed (p < 0.05). The mRNA levels of IL-1ß and IL-10 in the pre-HSCT group increased in the samples from day 7 (p < 0.05). The chemokine CCL-2/MCP-1 was not detected in pre-HSCT group. Chemokine receptor CXCR4 levels increased in samples obtained from the day 7 in the control group (p < 0.05). CONCLUSIONS: Individuals undergoing HSTC presented similar cytokine and chemokine mRNA expression compared with healthy individuals. However, it was observed the total absence of mRNA MCP-1/CCL2 expression in those individuals undergoing HSCT. CLINICAL RELEVANCE: Patients undergoing HSCT are at higher risk of infection. No study has analysed the periapical immune responses to root canal infections in HSCT individuals.
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Trasplante de Células Madre Hematopoyéticas , Periodontitis Periapical , Citocinas , Necrosis de la Pulpa Dental , Humanos , Periodontitis Periapical/terapia , Tejido Periapical , Tratamiento del Conducto RadicularRESUMEN
OBJECTIVES: To evaluate the selenium (Se) behavior when used as an endodontic dressing in teeth with pulp necrosis. Additionally, its effects was also compared with the calcium hydroxide (C.H.), which is used globally as a root canal dressing, and the combination of the C.H. with Se (C.H. + Se). MATERIALS AND METHODS: The sample consisted of 60 patients requiring endodontic treatment who were divided into groups, i.e., without intracanal medication (empty) and with medications as follows: selenium (Se), calcium hydroxide (C.H.), and calcium hydroxide + selenium (C.H. + Se) (n = 15). After the coronary opening, three absorbent paper points were placed in the RCS and maintained for 2 min for microbial evaluation. Following the cleaning and shaping procedures, new paper points were introduced into the root canal system, passing passively through the root apex (2 mm) into the periapical tissues for 2 min, for immune evaluation. The collections were performed again 15 days later. Real-time PCR quantified the expression of the prokaryotic 16S ribosomal RNA. The 16S mRNA was evaluated before the cleaning and shaping procedures and 15 days later in the groups treated with or without medication. RESULTS: A significant reduction in the microbial load was observed only in the groups that received endodontic dressing (p < 0.05). The cytokines IFN-γ, TNF-α, IL-1α, IL-17A, IL-10, IL-6 and MCP-1, were also quantified by real-time PCR. There was an increase in the gene expression level of the cytokines (T15) TNF-α and IL-10 in the C.H. group compared to the other groups (p < 0.05). The IFN-γ mRNA expression was reduced in the groups treated with the medications (Se, C.H., and C.H. + Se). CONCLUSIONS: The findings of the present study indicate that in the case of treatment over multiple sessions, the use of root canal dressing is essential to avoid the root canal system (RCS) microbial recolonization. Selenium potentiated the effects of calcium hydroxide inducing an anti-inflammatory response in periapical tissues. CLINICAL RELEVANCE: Se is a mineral essential for the formation of the amino acid selenocysteine, which is directly involved in the maintenance of the immune response. Selenium has been widely used in the medical field in the treatment of cancer, as an activator of bone metabolism, and as a stimulator of the immune system. In this study, it was shown that the incorporation of Se, whether as intracanal medication alone or in conjunction with other medications, may potentiate periapical tissue repair after RCS cleaning and shaping procedures.
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Periodontitis Periapical , Selenio , Vendajes , Hidróxido de Calcio/farmacología , Cavidad Pulpar , Necrosis de la Pulpa Dental , Humanos , Inmunidad , Periodontitis Periapical/terapia , Tejido Periapical , Irrigantes del Conducto Radicular , Selenio/farmacologíaRESUMEN
The aim of this study was to explore the microbial communities of endodontic infections at their apical portion by 16S rRNA Illumina sequencing and delineate the core microbiome of root canal infections and that of their associated clinical symptomatology. Samples were collected from fifteen subjects presenting one tooth with a root canal infection, and their associated symptoms were recorded. Samples were collected from the apical third of roots using a #10 K file and then amplified using multiple displacement amplification and PCR-amplified with universal primers. Amplicons were sequenced (V3-V4 hypervariable region of the 16S rRNA gene) using MiSeq (Illumina, CA). The microbial composition of the samples was determined using QIIME and HOMINGS. Data were analyzed using t tests and ANOVA. A total of 1,038,656 good quality sequences were obtained, and OTUs were assigned to 10 bacterial phyla, led by Bacteroidetes (51.2%) and Firmicutes (27.1%), and 94 genera were represented primarily by Prevotella (17.9%) and Bacteroidaceae G-1 (14.3%). Symptomatic teeth were associated with higher levels of Porphyromonas (p < 0.05) and Prevotella. P. endodontalis and P. oris were present in both cores. The present study demonstrated the complexity of the root canal microbiome and the "common denominators" of root canal infections and identified taxa whose virulence properties should be further explored. The polymicrobial etiology of endodontic infections has long been established. However, few studies have focused on expanding the breadth and depth of coverage of microbiome-infected root canals at their apical portion.
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Bacterias/aislamiento & purificación , ADN Bacteriano/genética , Cavidad Pulpar/microbiología , Secuenciación de Nucleótidos de Alto Rendimiento , Microbiota , Adolescente , Adulto , Anciano , Bacterias/clasificación , Bacterias/genética , Niño , ADN Bacteriano/análisis , Femenino , Infección Focal Dental/microbiología , Humanos , Masculino , Persona de Mediana Edad , ARN Bacteriano/análisis , ARN Bacteriano/genética , ARN Ribosómico 16S/genética , Ribotipificación , Especificidad de la Especie , Adulto JovenRESUMEN
OBJECTIVES: To identify the gene expression of the cytokines IL-9, TNF-α, IL-1, INF-γ, IL-17A, and IL-10 and the chemokines CCL-2/MCP-1 and CCR-6 in the periapical fluid of human root canal infections. MATERIALS AND METHODS: Twenty samples were collected immediately and 7 days after the cleaning and shaping procedures (after reducing the intracanal microbial load) in an attempt to characterize the expression of these genes. The endogenous expression levels of cytokines and chemokines were analyzed by real-time polymerase chain reaction. The Shapiro-Wilk and the Wilcoxon tests analyzed data. RESULTS: Significantly higher levels of the IL-9, INF-γ, TNF-α, IL-1, and IL-10 markers on day 7 were observed compared with day 0 (p < 0.05). However, IL-17A and the chemokines CCL-2/MCP-1 and CCR-6 did not show a significant difference in mRNA expression when comparing both timepoints (p > 0.05). CONCLUSIONS: The clinical variation of the periapical immune status after endodontic therapy suggests that the cytokine and chemokine-mediated pro-inflammatory response appears to be modulated in an IL-10/IL-9-dependent manner. CLINICAL RELEVANCE: Few studies have investigated the role of Th9 cells in periapical lesions. IL-9 presents exciting plasticity, performing immunosuppressive actions, and it is also capable of changing their phenotype in the presence of IL-17. Hence, it is relevant to investigate its role in the context of the known mediators involved the periapical immune process.
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Expresión Génica , Quimiocinas , Citocinas , Humanos , Tratamiento del Conducto Radicular , Factor de Necrosis Tumoral alfaRESUMEN
INTRODUCTION: The purpose of this study was to combine multiple displacement amplification and checkerboard DNA-DNA hybridization to qualitatively and quantitatively evaluate the microbiota present in infections refractory to endodontic treatment. METHODS: The subjects of this study were 40 patients presenting with periapical lesions refractory to endodontic treatment. Samples were taken by scraping or filing root canal walls with a #10 K-type hand file. Sample DNA was amplified by multiple displacement amplification, and the levels of 107 bacterial taxa were analyzed by checkerboard DNA-DNA hybridization. The taxa were divided into 3 distinct microbial populations depending on their mean proportion in samples (% DNA probe counts ± standard error of the mean) as follows: dominant (≥3.0%), subdominant (>1.6%-3.0%), and residual (≤1.6%) populations. The significance of differences was determined using the Mann-Whitney test. RESULTS: The taxa present with the highest mean proportions (constituting the dominant population) were Corynebacterium diphtheriae (8.03 ± 0.98), Porphyromonas gingivalis (5.42 ± 2.09), Streptococcus sobrinus (5.33 ± 0.69), and Stenotrophomonas maltophilia (4.72 ± 1.73). Among the subdominant population were Eubacterium saphenum (3.85 ± 1.06), Helicobacter pylori (3.16 ± 0.62), Dialister pneumosintes (3.12 ± 1.1), Clostridium difficile (2.74 ± 0.41), Enterobacter agglomerans (2.64 ± 0.54), Salmonella enterica (2.51 ± 0.52), Mobiluncus mulieris (2.44 ± 0.6), and Klebsiella oxytoca (2.32 ± 0.66). In the population of bacteria present at the lowest mean proportions (the residual population), Bacteroides ureolyticus (0.04 ± 0.01), Haemophilus influenzae (0.04 ± 0.02), and Prevotella oris (0.01 ± 0.01) were found at the lowest mean proportions. Enterococcus faecalis was detected in the residual population (0.52 ± 0.26). CONCLUSIONS: The microbial climax community in teeth refractory to endodontic treatment not only harbors medically important species but also contains distinct microbial consortia present with different population levels.
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Bacterias/aislamiento & purificación , Cavidad Pulpar/microbiología , Enfermedades Periapicales/microbiología , Tratamiento del Conducto Radicular , Sondas de ADN , ADN Bacteriano/análisis , Necrosis de la Pulpa Dental/microbiología , Humanos , Microbiota , Hibridación de Ácido NucleicoRESUMEN
INTRODUCTION: The purpose of this study was to examine alpha-2 integrin, molecular mediators, cytokines, and chemokines from cells in periapical interstitial fluid from root canal infections before and after the reduction of the bacterial load using a cleaning procedure. METHODS: Subjects included 20 patients referred to the School of Dentistry at the Universidade Federal de Minas Gerais (Belo Horizonte, Minas Gerais, Brazil). Clinical samples were taken from teeth with pulp necrosis, and no patients had acute periapical symptoms at the time of the appointments. After cleaning and drying, 3 paper points were introduced into the root canal, passing passively through the root apex (2 mm) into the periapical tissues for 1 minute. The samples were collected immediately after root canal cleaning and 7 days later (restrained root canal bacterial load) to characterize those gene expressions using real-time polymerase chain reaction. RESULTS: Significantly lower levels of tumor necrosis factor alpha, chemokine ligand 5 (CCL5), chemokine ligand 2/monocyte chemotactic protein 1 (CCL2/MCP-1), and interleukin (IL)-8 in teeth with restrained bacterial loads (second collection) compared with the first collection were observed (P < .05). Similarly, the messenger RNA expression of the integrins secreted phosphoprotein 1 (SSP1)/ostepontin and focal adhesion kinase (FAK) decreased in samples from the second collection (P < .05). The messenger RNA for the regulatory cytokine IL-10 was significant higher in samples from the second collection (day 7) compared with the first collection (day 0) (P < .05). Messenger RNA expression of IL-1ß, IL-17A, interferon gamma, alpha-2 integrin, and Hsp47/SERPINH1 were similar at both time points (P > .05). CONCLUSIONS: These findings suggest that after reducing the root canal bacterial load a decrease in the inflammatory response took place in the periapical lesions.
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Infecciones Bacterianas/terapia , Citocinas/metabolismo , Proteínas de Choque Térmico/metabolismo , Integrina alfa2/metabolismo , Periodontitis Periapical/terapia , Tejido Periapical/metabolismo , Infecciones Bacterianas/inmunología , Infecciones Bacterianas/metabolismo , Carga Bacteriana , Humanos , Periodontitis Periapical/inmunología , Periodontitis Periapical/metabolismo , Periodontitis Periapical/microbiología , ARN Mensajero/metabolismo , Tratamiento del Conducto RadicularRESUMEN
INTRODUCTION: Sickle cell anemia (SCA) is the most prevalent genetic disease worldwide. Patients with SCA exhibit increased levels of proinflammatory mediators as part of a permanently activated immunoinflammatory status. METHODS: The aim of this study was to evaluate the mRNA expression levels of the cytokines interferon (IFN-γ), tumor necrosis factor, interleukin (IL-1ß, IL-17A, IL-10), receptor activator for nuclear factor kappa B ligand, and the chemokines CCL2/MCP-1 and CCL5 in the periapical interstitial fluid from SCA individuals compared with healthy individuals. Samples were collected from 12 teeth of SCA patients and 12 non-SCA patients with apical periodontitis. In addition, 12 teeth were sampled from the periapical region of healthy patients with vital pulp (control). The expression of cytokine mRNA was detected by using real-time polymerase chain reaction. RESULTS: The expression of mRNA for the Th1-associated cytokines IFN-γ, tumor necrosis factor-α, and IL-1ß were significantly higher in SCA individuals than in the control individuals (P < .05). Among Th1-associated cytokines, only IFN-γ was significantly increased in non-SCA compared with control patients (vital pulp). The expression of IL-17A mRNA was significant higher in SCA cases than in control samples (P < .05), whereas the IL-10 mRNA expression was significantly increased in SCA and non-SCA individuals when compared with the control group. Similar levels of receptor activator for nuclear factor kappa B ligand, CCL2, and CCL5 mRNA expression were observed in all samples. However, no significant differences were observed in the expression of cytokine or chemokine mRNA between SCA and non-SCA individuals (P > .05). CONCLUSIONS: The results were able to demonstrate that SCA patients presented prone proinflammatory ability, despite the fact that any differences in periapical immune responses between SCA and non-SCA individuals were not observed.
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Anemia de Células Falciformes/metabolismo , Citocinas/metabolismo , Tejido Periapical/metabolismo , Anemia de Células Falciformes/genética , Estudios de Casos y Controles , Citocinas/genética , Regulación de la Expresión Génica , HumanosRESUMEN
INTRODUCTION: Root canal treatment typically involves cleaning and shaping procedures followed by treatment with antibacterial endodontic dressing between appointments and, ultimately, 3-dimensional,hermetic filling. Chlorhexidine (CHX) is effective as an irrigation solution and is used as an endodontic dressing. The aim of this study was to examine the influence of CHX on periapical cytokine expression. METHODS: Expression levels of the cytokines interferon γ, tumor necrosis factor α, interleukin (IL)-1ß, IL-17A, IL-10, and the chemokine monocyte chemoattractant protein-1 (CCL2/MCP-1) were assayed by real-time polymerase chain reaction immediately after root canal cleaning and 15 days later. RESULTS: Messenger RNA expression of IL-1ß, interferon γ, IL-10, and CCL2/MCP-1 was increased on day 15 in teeth without endodontic dressing. No statistical change was observed in the messenger RNA expression of cytokines when comparing sampling times for teeth that received endodontic dressing. CONCLUSIONS: The results show that CHX application between appointments prevented the increase of both proinflammatory and immunoregulatory cytokines 15 days after the dental procedure.
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Clorhexidina/farmacología , Citocinas/efectos de los fármacos , Tejido Periapical/efectos de los fármacos , Irrigantes del Conducto Radicular/farmacología , Preparación del Conducto Radicular/métodos , Diente/efectos de los fármacos , Quimiocina CCL2/análisis , Citocinas/análisis , Necrosis de la Pulpa Dental/inmunología , Necrosis de la Pulpa Dental/terapia , Estudios de Seguimiento , Humanos , Mediadores de Inflamación/análisis , Interferón gamma/efectos de los fármacos , Interleucina-1/análisis , Interleucina-10/análisis , Interleucina-17/análisis , Tejido Periapical/inmunología , ARN Mensajero/efectos de los fármacos , Diente/inmunologíaRESUMEN
INTRODUCTION: The use of calcium hydroxide is an effective step in killing bacteria that remain after cleaning and shaping procedures. It also induces hard-tissue formation and is effective for stopping inflammatory exudates. METHODS: The aim of this study was to assay and to compare the influence of calcium hydroxide on periapical interstitial fluid from human root canals. The mRNA expression levels of the cytokines interferon (IFN)-γ, tumor necrosis factor-α, interleukin (IL)-1ß, IL-17A, and IL-10 as well as the chemokine MCP-1 were assayed by real-time polymerase chain reaction immediately after root canal cleaning and 15 days later. RESULTS: Levels of IL-1ß, IFN-γ, IL-10, and the chemokine CCL2/MCP-1 were increased in teeth without endodontic dressings. With calcium hydroxide interappointment dressings, no statistically significant changes were observed in cytokine mRNA expression. However, when comparing teeth that received the medication with those that did not, expression levels of IL-1ß, IFN-γ, and IL-10 were statistically lower in those teeth that received calcium hydroxide. CONCLUSIONS: Analyses of cytokines and the chemokine CCL-2/MCP-1 demonstrated the benefits of calcium hydroxide as a root canal dressing because it impedes the increase of all mediators during the experimental time.
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Hidróxido de Calcio/uso terapéutico , Citocinas/biosíntesis , Necrosis de la Pulpa Dental/tratamiento farmacológico , Necrosis de la Pulpa Dental/metabolismo , Irrigantes del Conducto Radicular/uso terapéutico , Quimiocina CCL2/biosíntesis , Humanos , Interferón gamma/biosíntesis , Interleucina-10/biosíntesis , Interleucina-1beta/biosíntesis , ARN Mensajero/biosíntesis , Reacción en Cadena en Tiempo Real de la Polimerasa , Estadísticas no ParamétricasRESUMEN
INTRODUCTION: Lymphocytes, among many cells, express different sets of cytokines, chemokines, and receptors, which are considered important mediators of periapical immune response to infection. METHODS: The aim of this study was to evaluate the mRNA expression of CD4(+)CD28(+) and CD8(+) T genes and the gene expression of interferon-γ, tumor necrosis factor-α, interleukin (IL)-1ß, IL-17A, IL-10, CCL2/MCP-1, CCL4, CCL5, CXCR4, CCR5, and receptor activator for nuclear factor kappa B ligand (RANKL) in periapical interstitial fluid from human root canal infections. The samples were collected immediately after root canal cleaning and 7 days later (restrained root canal bacterial load) to characterize those gene expressions. RESULTS: Real-time polymerase chain reaction demonstrated significantly higher levels of CD4(+)CD28(+) and CD8(+) T-cell markers in the former root canal condition and an increase of IL-10 and CXCR4, followed by a decrease of proinflammatory cytokines such as RANKL, interferon-γ, IL-1ß, and CCL5. CONCLUSIONS: Analyses of T-lymphocyte and cytokine expression in periapical area were able to show that distinct root canal conditions might play regulatory roles in controlling local immune/inflammatory processes.
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Citocinas/análisis , Periodontitis Periapical/inmunología , Linfocitos T/inmunología , Infecciones Bacterianas/inmunología , Antígenos CD28/análisis , Antígenos CD4/análisis , Linfocitos T CD4-Positivos/inmunología , Antígenos CD8/análisis , Linfocitos T CD8-positivos/inmunología , Quimiocina CCL2/análisis , Quimiocina CCL4/análisis , Quimiocina CCL5/análisis , Necrosis de la Pulpa Dental/inmunología , Necrosis de la Pulpa Dental/microbiología , Líquido Extracelular/inmunología , Estudios de Seguimiento , Humanos , Mediadores de Inflamación/análisis , Interferón gamma/análisis , Interleucina-10/análisis , Interleucina-17/análisis , Interleucina-1beta/análisis , Ligando RANK/análisis , Receptores CCR5 , Receptores CXCR4 , Tratamiento del Conducto Radicular , Factor de Necrosis Tumoral alfa/análisisRESUMEN
INTRODUCTION: Failure in endodontic treatment is often caused by the persistence of microorganisms in the root canal after therapy. When treatment fails, an immune response develops that is characterized by an extensive network of immunologic mechanisms that lead to the production of cytokines and chemokines. METHODS: The objective of this study was to determine the relative messenger RNA (mRNA) expression of IFN-γ, TNF-α, IL-1ß, IL-17A, IL-10, and MCP-1 in periapical dental lesions refractory to treatment. Clinical samples were taken from teeth presenting periapical lesions refractory to endodontic treatment (the experimental group) or from healthy teeth with pulp vitality (the control group). Three paper points passing through the root apex (2 mm) were used to collect the samples. The total RNA was extracted from each sample, complementary DNA was synthesized, and quantitative polymerase chain reaction analysis was performed. The Mann-Whitney U test was used to determine the statistical significance of our findings (P < .05). RESULTS: Significant differences in the levels of IFN-γ, TNF-α, IL-17A, and MCP-1 mRNA expression were observed in cases refractory to endodontic treatment as compared with the control group. The expression of IL-1ß mRNA was not significantly different between the groups. The expression of IL-10 mRNA was insignificant in both the experimental and control groups. CONCLUSIONS: A significantly increased expression of TNF-α, IFN-γ, IL-17A, and MCP-1 mRNA was observed in the periapical immune response in cases of endodontic failure. These results suggest that a proinflammatory cytokine profile predominates in these types of dental lesions.
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Citocinas/análisis , Enfermedades Periapicales/inmunología , Tratamiento del Conducto Radicular , Adolescente , Adulto , Quimiocina CCL2/análisis , Pulpa Dental/inmunología , Femenino , Humanos , Interferón gamma/análisis , Interleucina-10/análisis , Interleucina-17/análisis , Interleucina-1beta/análisis , Masculino , Persona de Mediana Edad , ARN Mensajero/análisis , Reacción en Cadena en Tiempo Real de la Polimerasa , Recurrencia , Ápice del Diente/inmunología , Insuficiencia del Tratamiento , Factor de Necrosis Tumoral alfa/análisis , Adulto JovenAsunto(s)
Inmunidad Innata/efectos de los fármacos , Macrófagos Peritoneales/efectos de los fármacos , Materiales de Obturación del Conducto Radicular/toxicidad , Animales , Infecciones Bacterianas/inmunología , Adhesión Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Femenino , Interleucina-10/biosíntesis , Interleucina-12/biosíntesis , Macrófagos Peritoneales/inmunología , Macrófagos Peritoneales/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Óxido Nítrico/biosíntesis , Fagocitosis/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismo , Saccharomyces/inmunología , Factor de Necrosis Tumoral alfa/biosíntesisRESUMEN
INTRODUCTION: The effects of highly active antiretroviral therapy (HAART) on the prevalence of endodontic diseases in HIV-infected individuals have not been reported. METHODS: The purpose of this study was to determine and compare sex, age, need for root canal treatment, CD4 and CD8 lymphocyte counts, HIV viral load, and antiretroviral therapy in 222 HIV-infected individuals who were monitored at an HIV Reference Center in Brazil. RESULTS: One hundred sixty-six patients comprised the studied population; the rate of the need for endodontic treatment was 14.5%. There was a positive correlation between the CD4 + T-cells count (< 500 cell/ mm(3)) and the need for endodontic treatment, but there was no statistical difference between the need for endodontic treatment and the HIV viral load or the CD4+/CD8+ ratio in T-cell counts. Of the population that needed endodontic treatment, 79.2% was taking the HAART regimen, and the majority of the studied population was female. CONCLUSION: We found that those HIV-infected individuals needing endodontic treatment had a lower CD4 + T-cell count, which probably correlated with a lower adherence to the HAART regimen.