Accessing the Health Risk of Ingestion of Surface Water from Lucrécia and Parelhas Dams in Northeast Brazil Using the Sentinel Organism Drosophila melanogaster.

This study investigated the genotoxic risk of chronic exposure of hemolymph's cells of Drosophila melanogaster (Insecta, Diptera) to water samples from Boqueirão de Parelhas Dam and from Lucrécia Dam in the semiarid region of Brazil. The dams are located over the Pegmatite Province of Borborema, with rocks rich in uranium and thorium. Water samples hydrated a culture medium composed of mashed potatoes, where larvae of D. melanogaster fed for 24 h, before be underwent to the Comet assay. The same water was evaluated for the presence of dissolved Radon gas (222Rn) and concentrations of 11 toxic metals (Ag, Al, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb and Zn). The results indicated a genotoxic effect resulting from exposure to the waters of the Parelhas dam, in the samples of August 2018; and in Lucrécia dam, in January 2019. D. melanogaster stood out for its high sensitivity to monitor the genotoxic effects of compounds dissolved in public dams. And unlike to other essentially aquatic sentinel organisms, this species stood out as a model to concomitant studies of air and water possible contaminated, in a scenario of natural environmental radioactivity present in semiarid of Brazil.

A Review of Analytical Methods for Calcium Salts and Cholecalciferol in Dietary Supplements.

Dietary supplements composed by the combination of a calcium salt with cholecalciferol (vitamin D3) are widely used for improving bone health in conditions caused by the deficiency of these compounds in the body. Historically, these supplements have been linked to quality and safety issues. In the case of calcium salts, the presence of potentially toxic contaminants such as lead (Pb) has already been alerted by health authorities from different countries. Meanwhile, cholecalciferol is very unstable under inadequate manufacturing and storage conditions. The content of both compounds in commercial dietary supplements is often found to be in disagreement with the label claims, which can lead to a deficient or excessive nutrient intake by consumers. In this scenario, analyzing these compounds is still a difficult and time-consuming task, which usually requires specific pretreatment procedures and multiple analytical methods due to the inorganic nature of calcium and the organic nature of cholecalciferol. Therefore, this article reviews the analytical methods, described in official compendia and scientific literature, for the determination of calcium salts and cholecalciferol in dietary supplement formulations. We also approached the sample preparation procedures highly required due to the matrix complexity of these materials.

Toxicity and genotoxicity of domestic sewage sludge in the freshwater snail Biomphalaria glabrata (Say, 1818).

Waste produced in homes is one of the main sources of pollutants in freshwater ecosystems. Therefore, it is imperative to implement methodologies that aid in environmental monitoring procedures. The use of organisms as biomonitors has grown increasingly prevalent as they are models that provide data that can be adequately evaluated. In this work, we investigated the genotoxic and cytotoxic effects caused by domestic sewage sludge through an analysis of biomarkers in the mollusk Biomphalaria glabrata. For the tests, increasing concentrations of 50, 100, 150, and 500 mg L-1 of domestic sewage sludge were standardized, in addition to control groups. Assays were performed after the mollusks were exposed to the domestic sewage sludge in acute (48 h) and chronic (15 d) manner. Toxicity tests were performed with embryonic and adult snails. The cytoplasmic and nuclear changes were analyzed in the hemocyte cells. Lastly, genotoxic damage was analyzed using the comet assay. Adult snails and embryos of B. glabrata showed no significant morphological changes. Domestic sludge caused deleterious effects on mollusks as confirmed after cell genotoxicity tests. Therefore, based on the results obtained from the analysis of B. glabrata hemocytes, we can affirm that domestic sewage sludge causes genotoxic and cytotoxic effects on mollusk cells. Therefore, it is possible to conclude that the mollusk Biomphalaria glabrata can be used as a good low-cost alternative to assist in the biomonitoring of freshwater environments. Graphical Abstract.

Historical inputs of polycyclic aromatic hydrocarbons in the preserved tropical estuary of the Itapicuru River, Bahia, Brazil.

The aim of present study was to evaluate temporal changes in the distribution of polycyclic aromatic hydrocarbons (PAHs) in the estuary of the Itapicuru (Brazil). A sediment core was sampled in the study area. Concentrations of the 16 priority PAHs were analyzed using gas chromatography coupled to a mass spectrometry. A gas flow proportional counter was used to estimate the sedimentation rate through the determination of 210Pb. Granulometric fractions and total organic carbon (TOC) concentrations were also evaluated. Concentrations of TOC and PAHs ranged from 0.65 to 2.51% and 1.98 to 43.1 ng g-1 (dry weight), respectively. Significant correlations (p < .05) were found between the mud content in the sediment core samples and concentrations of both TOC and PAHs. Higher PAH concentrations occurred after the 1950s. The main sources of PAHs over time were local human activities on the northern coast of the state of Bahia.

Studies on Toxicity of Suspensions of CdTe Quantum Dots to Biomphalaria glabrata Mollusks.

Quantum dots have generated great interest because of their optical properties, both to life sciences and electronics applications. However, possible risks to the environment associated with these nanoparticles are still under investigation. The present study aimed to evaluate the toxicity of suspensions of cadmium telluride (CdTe) quantum dots to Biomphalaria glabrata mollusks, a very sensitive aquatic environmental bioindicator for physical and chemical agents. Toxicity was examined by using embryos and adult mollusks as well as hemocytes. The distribution of cadmium in the organs of adults was also assessed. Effects of the stabilizing agent of the quantum dots were also evaluated. Animals were exposed to suspensions of quantum dots for 24 h, at concentrations varying from 1.2 to 20 nM for embryos and from 50 to 400 nM for adult mollusks. Results showed that suspensions of quantum dots induced malformations and mortality in embryos and mortality in adults, depending on the concentration applied. In the cytotoxicity study, hemocyte apoptosis was observed in adults exposed to the highest concentration of quantum dots applied as well as to the stabilizing agent. Cell binucleation and micronucleus frequencies were not significative. Bioaccumulation evaluation revealed that quantum dots targeted the digestive gland (hepatopancreas). Taken together, outcomes suggested that specific nano-effects related directly not only to composition but also to the aggregation of quantum dots may be mediating the observed toxicity. Thus B. glabrata was determined to be a very sensitive species for interpreting possible nano-effects in aquatic environments. Environ Toxicol Chem 2019;38:2128-2136. © 2019 SETAC.

Study of the Potential Radiomitigator Effect of Quercetin on Human Lymphocytes.

Several substances of synthetic and natural origin have been studied to determine their ability to protect the body from damage caused by ionizing radiation. Among these substances, quercetin has been shown to be a naturally occurring molecule with high radioprotective and radiomitigator potential due to its antioxidant properties. The objective of this work was to ascertain the potential radiomitigator effect of quercetin on chromosome aberration yield in lymphocytes of in vitro-irradiated human peripheral blood. At first, the DPPH (2,2-diphenyl-1-picryl-hydrazyl) radical capture test was performed to determine the antioxidant activity of quercetin and to select the concentrations to be tested. The blood was irradiated at doses of 2.5, 3.5, and 4.5 Gy and lymphocytes were cultured with quercetin at preselected concentrations of 37.5 and 75 µM. Then, the slides were prepared for scoring unstable chromosome aberrations (dicentrics, rings, and fragments). The results showed that the lymphocytes irradiated and later exposed to quercetin presented a lower frequency of chromosomal alterations compared to the control sample which was irradiated and not exposed to quercetin. The results suggest a potential radiomitigator effect of the flavonoid quercetin on human lymphocytes exposed, in vitro, to ionizing radiation. This effect may be related to decrease in the release of cytokines (INF-γ, PGE2, IL-1ß, IL6, IL-8) involved in the proinflammatory processes as well as downregulation of NF-kB and reduction of expression TGF-ß.

Cytotoxic and genotoxic effect of oxyfluorfen on hemocytes of Biomphalaria glabrata.

Chemicals released from anthropogenic activities such as industry and agriculture often end up in aquatic ecosystems. These substances can cause serious damage to these ecosystems, thus threatening the conservation of biodiversity. Among these substances are pesticides, such as oxyfluorfen, a herbicide used for the control of grasses and weeds. Considering its widespread use, it is important to investigate the possible toxicity of this compound to aquatic organisms, especially invertebrates. Hence, the use of biological systems able to detect such effects is of great importance. The mollusk Biomphalaria glabrata has been shown to be useful as an environmental indicator to assess the potential ecological effects of physical and chemical stressors in freshwater environments. The present study sought to detect mutagenic changes in hemocytes of B. glabrata exposed to oxyfluorfen. To perform these tests, this study used ten animals per group, exposed acutely (48 h) and chronically (15 days) to oxyfluorfen. The herbicide concentrations were 0.125, 0.25, and 0.5 mg/L. The results showed that oxyfluorfen induced significant frequencies of micronuclei, binucleated cells, and apoptosis in hemocytes of mollusks when compared to the control group. Unlike chronic exposure, acute exposure was dose-dependent. The present study's results demonstrate the cytotoxic and genotoxic effects of oxyfluorfen on hemocytes of B. glabrata.

Drosophila melanogaster as model organism for monitoring and analyzing genotoxicity associated with city air pollution.

This study evaluated the genotoxic potential of atmospheric pollution associated with urbanization using the model organism Drosophila melanogaster and the Comet assay with hemolymph cells. Larvae were exposed to atmospheric compounds in an urban and a rural area in the municipality of Vitória de Santo Antão, Pernambuco, Brazil, for 6 days (from the embryo stage to the third larval stage) in April 2015 and April 2017. The results were compared to a negative environmental control group exposed to a preserved area (Catimbau National Park) and to a negative control exposed to the laboratory room conditions. The Comet assay demonstrated significant genetic damage in the organisms exposed to the urban area compared with those exposed to the rural area and negative control groups. The evidences were supported by particulate matter analysis showing higher photopeaks of chemical elements such as aluminum, silicon, sulfur, potassium, calcium, titanium, and iron, associated to road dust fraction in urban environment. Once again, the results confirm D. melanogaster an ideal bioindicator organism to monitor genotoxic hazard associated with atmospheric pollution.

EDXRF as an alternative method for multielement analysis of tropical soils and sediments.

The quality assessment of tropical soils and sediments is still under discussion, with efforts being made on the part of governmental agencies to establish reference values. Energy dispersive X-ray fluorescence (EDXRF) is a potential analytical technique for quantifying diverse chemical elements in geological material without chemical treatment, primarily when it is performed at an appropriate metrological level. In this work, analytical curves were obtained by means of the analysis of geological reference materials (RMs), which allowed for the researchers to draw a comparison among the sources of analytical uncertainty. After having determined the quality assurance of the analytical procedure, the EDXRF method was applied to determine chemical elements in soils from the state of Pernambuco, Brazil. The regression coefficients of the analytical curves used to determine Al, Ca, Fe, K, Mg, Mn, Ni, Pb, Si, Sr, Ti, and Zn were higher than 0.99. The quality of the analytical procedure was demonstrated at a 95% confidence level, in which the estimated analytical uncertainties agreed with those from the RM's certificates of analysis. The analysis of diverse geological samples from Pernambuco indicated higher concentrations of Ni and Zn in sugarcane, with maximum values of 41 mg kg- 1 and 118 mg kg- 1, respectively, and agricultural areas (41 mg kg- 1 and 127 mg kg- 1, respectively). The trace element Sr was mainly enriched in urban soils with values of 400 mg kg- 1. According to the results, the EDXRF method was successfully implemented, providing some chemical tracers for the quality assessment of tropical soils and sediments.

Determination of Hg and diet identification in otter (Lontra longicaudis) feces.

An analytical procedure for the determination of Hg in otter (Lontra longicaudis) feces was developed, to separate fish scales for the identification of the animal diet. Samples were washed with ultra-pure water and the suspension was sampled and transferred for digestion. The solubilization was performed with nitric-perchloric acid mixture, and detection carried out by the atomic fluorescence spectrometry (AFS). The quality of the analytical procedure was assessed by analyzing in-house standard solutions and certified reference materials. Total Hg concentrations were in the range of 7.6-156 ng g(-1) (July 2004), 25.6-277 ng g(-1) (January 2005) and 14.6-744 ng g(-1) (May 2005) that is approximately the same order of magnitude for all samples collected in two reservoirs at the Tiete River, Brazil. Although Hg concentrations varied with sampling periods and diet, high levels were correlated to the percentage of carnivorous fish scales present in the otter feces.

Seleção de epífitas acumuladoras de elementos químicos na Mata Atlântica / Selection of epiphytes as accumulators of chemical elements in the Atlantic Forest

Epífitas são eficientes indicadores de poluição atmosférica devido à absorção de elementos químicos diretamente da atmosfera. Folhas de onze espécies de bromélias e uma espécie de orquídea foram coletadas no Parque Estadual Carlos Botelho, SP, para a determinação de dezesseis elementos químicos. A seleção foi baseada na diversidade e acumulação de elementos químicos encontrados nas folhas. Com um índice de acumulação de 0,7, a espécie Canistropsis billbergioides tem potencial para ser empregada como biomonitora de elementos químicos na Mata Atlântica.

Epiphytes are efficient indicators of atmospheric pollution because of their direct uptake of chemical elements from atmosphere. Leaves from eleven species of bromeliads and one species of orchid were collected in the Parque Estadual Carlos Botelho, SP, for determination of sixteen chemical elements. The selection of the best biomonitor species was based on the diversity and accumulation of chemical elements in the leaves. With an average accumulation index of 0.7, Canistropsis billbergioides has potential to be used as a biomonitor of chemical elements in the Atlantic Forest.