RESUMEN
Thermal instability, photodegradation, and poor bioavailability of natural active ingredients are major drawbacks in developing effective natural product-based antimicrobial formulations. These inherited issues could be fruitfully mitigated by the introduction of natural active ingredients into various nanostructures. This study focuses on the development of a novel green mechanochemical synthetic route to incorporate curcuminoids into Mg-Al-layered double hydroxides. The developed one-pot and scalable synthetic approach makes lengthy synthesis procedures using toxic solvents redundant, leading to improved energy efficiency. The hydrotalcite-shaped nanohybrids consist of surface and interlayer curcuminoids that have formed weak bonds with layered double hydroxides as corroborated by X-ray diffractograms, X-ray photoelectron spectra, and Fourier transmission infrared spectra. The structural and morphological properties resulted in increased thermal stability of curcuminoids. Slow and sustained release of the curcuminoids was observed at pH 5.5 for a prolonged time up to 7 h. The developed nanohybrids exhibited zeroth-order kinetics, favoring transdermal application. Furthermore, the efficacy of curcuminoid incorporated LDHs (CC-LDH) as an anticolonization agent was investigated against four wound biofilm-forming pathogens, Pseudomonas aeruginosa, Staphylococcus aureus, methicillin-resistant Staphyloccocus aureus, and Candida albicans, using a broth dilution method and an in vitro biofilm model system. Microbiological studies revealed a 54-58% reduction in biofilm formation ability of bacterial pathogens in developed nanohybrids compared to pure curcuminoids. Therefore, the suitability of these green-chemically synthesized CC-LDH nanohybrids for next-generation antimicrobial applications with advanced dermatological/medical properties is well established.
RESUMEN
During the last three decades, studies of linamarin extracted from cassava have received increased attention due to the presence of high cyanogenic compounds in these extracts. The methods that are utilized to isolate linamarin are either tedious or use acidic conditions resulting in poor yields. In this study, a novel cryocooled method of extraction has been developed to isolate linamarin from Cassava root peel. Approximately 18 g of linamarin was isolated from 1 kg of fresh Cassava root peel, which is the highest amount reported to date. Linamarin was fully characterized using NMR, IR and LCMS. The anti-cancer properties of pure linamarin and Cassava crude extract were evaluated by a comprehensive cytotoxic assay, using MCF-7, HepG2, NCI H-292, AN3CA and MRC-5 cell lines. The crude extract showed higher cytotoxicity compared to pure linamarin. The results of the biological evaluation are comparable to other reported studies in the literature.
Asunto(s)
Manihot , Nitrilos , VerdurasRESUMEN
Worldwide, there is an amplified interest in nanotechnology-based approaches to develop efficient nitrogen, phosphorus, and potassium fertilizers to address major challenges pertaining to food security. However, there are significant challenges associated with fertilizer manufacture and supply as well as cost in both economic and environmental terms. The main issues relating to nitrogen fertilizer surround the use of fossil fuels in its production and the emission of greenhouse gases resulting from its use in agriculture; phosphorus being a mineral source makes it nonrenewable and casts a shadow on its sustainable use in agriculture. This study focuses on development of an efficient P nutrient system that could overcome the inherent problems arising from current P fertilizers. Attempts are made to synthesize citric acid surface-modified hydroxyapatite nanoparticles using wet chemical precipitation. The resulting nanohybrids were characterized using powder X-ray diffraction to extract the crystallographic data, while functional group analysis was done by Fourier transform infrared spectroscopy. Morphology and particle size were studied using scanning electron microscopy along with elemental analysis using energy-dispersive X-ray diffraction spectroscopy. Its effectiveness as a source of P was investigated using water release studies and bioavailability studies using Zea mays as the model crop. Both tests demonstrated the increased availability of P from nanohybrids in the presence of an organic acid compared with pure hydroxyapatite nanoparticles and rock phosphate.
