Assessment of potential reference sites for evaluating the ecological status of subtropical and temperate Brazilian lotic systems using the epilithic diatom community.

This research aimed at determining reference sites in southern Brazilian lotic systems, using the Water Quality Trophic Index (WQTI), based on epilithic diatom communities. Within the period of 2012 and 2016, 140 epilithic diatom samples from seven sampling sites within the spring area of the Andreas Stream Hydrographic Basin, Vera Cruz County, RS, Brazil, were analyzed quarterly. Similarly, from 2012 and 2014, water samples in these sampling sites were collected for determination of physicochemical and microbiological parameters, such as water temperature, pH, turbidity, dissolved oxygen, biochemical oxygen demand, nitrate, phosphate, total dissolved solids, and fecal coliforms. The results indicated the occurrence of two diatom samples groups with significant differences (p < 0.05). Group 1 (sampling sites P1, P4, and P5) was classified in the oligotrophic category with an average WQTI of 1.3 ± 0.2, while group 2 (sampling sites P2, P3, P6, and P7) was classified in the ß-mesotrophic category with an average value of 2.0 ± 0.4). Achnanthidium minutissimum and Platessa hustedtii the dominant species were classified as oligotrophic taxa. Thus, we proposed the upper limit of the first interval of the WQTI score scale, equal to 1.5, as a reference value to differentiate the limits between the "high" and "good" ecological status to determine "reference sites" for subtropical and temperate Brazilian aquatic systems. The physical, chemical, and microbiological water quality evaluation gives support to this proposal, as far as the results indicated a significant improvement in the water quality, classifying sampling sites P1, P4, and P5 as having good quality appropriate for multiple uses.

Acute ecotoxicity and genotoxicity assessment of two wastewater treatment units.

Water contamination by discharge of untreated or poorly treated wastewater into water bodies is a current issue that may cause harm to humans. Water quality assessment targets general parameters, which often misleads to underestimation of their implication in the environment. Acute and genotoxicity assays using Daphnia magna is a reliable tool for testing deleterious effects of wastewater exposure. This paper aimed at evaluating acute ecotoxicity as well as genotoxicity of a biological treatment system composed by an anaerobic bioreactor (AR), algal turf scrubber (ATS), followed by two downflow constructed wetlands (CW). The university's wastewater treatment plant (WWTP) composed by an equalization tank (ET), an upflow anaerobic sludge blanket (UASB), followed by an aerobic bioreactor (AB) was also assessed for acute and genotoxicity. Our results showed the acute ecotoxicity ranged from moderately to extremely toxic, and from nontoxic to moderately toxic for ET and AB. For AR, most samples were moderately toxic. The outflow from ATS and CW's tanks completely eliminated acute toxicity and genotoxicity evidencing that the proposed system combining ATS and vertical CWs is suitable for treating sanitary wastewater.

Combined system for the treatment and reuse of urban wastewater: the efficiency of anaerobic reactors + hybrid constructed wetlands + ozonation.

The research developed a combined system in batch flow and in pilot scale for the treatment and reuse of urban effluents. The system was fed raw effluent from a university campus in Brazil and composed of four anaerobic reactors, three constructed wetlands (CWs) and an ozonation unit. The three sequential hybrid constructed wetlands were composed of a floating treatment wetland, an aerobic-anoxic baffled constructed wetland (CW) and a saturated vertifcal flow CW. Later, during the last trimester, weekly samples of the treated effluent were ozonated by bubbling with an application rate of 240 mg.h-1 O3. The system presented high removal rates efficiencies in terms of carbonaceous organic matter (78.9%), nitrogen (91.0%), color (96.7%) and turbidity (99.1%). In addition, it worked well for disinfection and acute ecotoxicity, but P was only efficiently (75%) removed in the first 8 months, with removing efficiency declining after this period. Ozonation provided significant color removal and an increased pH. The combination of floating, alternated upflow and downflow and saturated vertical flows improved the treatment of wastewater. This was due to the presence of both aerobic and anaerobic zones, as well as the filter substrate, through an integrated system with simple construction and operation and increased lifespan.

Algae turf scrubber and vertical constructed wetlands combined system for decentralized secondary wastewater treatment.

Water shortage is a current problem faced by many regions. The deterioration of water bodies driven by the directly discard of untreated wastewater worsens the water shortage and implies in more costly treatments to meet local standards for water quality. In rural areas, the problem is even worse, once conventional centralized treatment plants do not encompass them. Decentralized treatment systems must present low-cost, local availability, standards-meeting efficiency, and simplified operation. The present study examines the combined use of algae turf scrubber and down-flow vertical constructed wetlands for a University's sanitary wastewater treatment. After a hydraulic detention time of 21 days, the unit was able to reach 49%, 48%, 98%, 82%, 99.2%, 70.1%, 44%, 83%, 72%, 86%, 69%, 95%, and 99.9% for conductivity, total soluble solids, turbidity, apparent color, N-NH3, total nitrogen, P-soluble, total carbon, chemical oxygen demand, inorganic carbon, TOC, Escherichia coli, and total coliforms. In accord to the Brazilian standard ABNT 13969/97, the treated effluent is eligible for reuse in floor and sidewalks washing, garden irrigation, and landscaping purposes.