RESUMEN
OBJECTIVE: To determine continuities of mental health problems of children across a 24-year follow-up period. METHOD: In 1983, parent ratings of emotional and behavioral problems were collected with the Child Behavior Checklist (CBCL) in a general population sample of 2076 children. Twenty-four years later, 1365 participants completed Adult Self-Reports (ASR) to assess emotional and behavioral problems. RESULTS: Of the participants who were classified as deviant in childhood, 22.2% were also classified as deviant in adulthood. Both homotypic and heterotypic continuity was found. Childhood aggressive, delinquent, and anxious/depressed problems were associated with most adult psychopathology. Attention problems did not predict later problems independently. CONCLUSION: Even though assessed with parent-reports in childhood and analogous self-reports in adulthood, and over a large period of 24 years, continuity of psychopathology was found from childhood into adulthood. Anxious/depressed problems, delinquent behavior and aggressive behavior in childhood are core predictors for adult psychopathology.
Asunto(s)
Agresión , Trastorno de la Conducta/epidemiología , Trastorno de la Conducta/psicología , Delincuencia Juvenil/psicología , Delincuencia Juvenil/estadística & datos numéricos , Trastornos Mentales/epidemiología , Trastornos Mentales/psicología , Conducta Social , Adolescente , Adulto , Niño , Preescolar , Trastorno de la Conducta/diagnóstico , Trastorno Depresivo Mayor/diagnóstico , Trastorno Depresivo Mayor/epidemiología , Trastorno Depresivo Mayor/psicología , Femenino , Estudios de Seguimiento , Humanos , Masculino , Trastornos Mentales/diagnóstico , Índice de Severidad de la Enfermedad , Encuestas y Cuestionarios , Adulto JovenRESUMEN
The in vitro initiation of DNA replication was studied in permeable mammalian cells by a newly developed procedure. Pairs of monolayer cultures, one synchronized in G1 and the other in S phase, were incubated in a sandwich with assay solution, containing Triton X-100 for permeabilization and [3H]TTP as a tracer. After 1.5 h DNA synthesis was shown to be induced in 36 to 81% of the G1 nuclei. The inducing capacity of the S phase cultures was diminished by at least 50% after a 10 min exposure to 60 degrees C prior to incubation. The suitability application of this in vitro system for testing components that might effect the initiation of DNA replication is shown in an assay with G1 cultures where the addition of up to 1 mM Ap4A led to an increase of DNA synthesizing cells from 4 to 15%.