Novel Perspectives on Food-Based Natural Antimicrobials: A Review of Recent Findings Published since 2020.

Various fruit and vegetable wastes, particularly peels, seeds, pulp, and unprocessed residues from the food industry, are abundant sources of antioxidants and essential antimicrobial agents. These valuable bioactive compounds recovered from the food industry have a great application in food, agriculture, medicine, and pharmacology. Food-derived natural antimicrobials offer advantages such as diminishing microbial loads and prolonging the shelf life of food products particularly prone to microbial spoilage. They not only enrich the foods with antioxidants but also help prevent microbial contamination, thereby prolonging their shelf life. Similarly, incorporating these natural antimicrobials into food packaging products extends the shelf life of meat products. Moreover, in agricultural practices, these natural antimicrobials act as eco-friendly pesticides, eliminating phytopathogenic microbes responsible for causing plant diseases. In medicine and pharmacology, they are being explored as potential therapeutic agents. This review article is based on current studies conducted in the last four years, evaluating the effectiveness of food-based natural antimicrobials in food, agriculture, medicine, and pharmacology.

Evaluation of bioactivities of Pistacia vera L. hull extracts as a potential antimicrobial and antioxidant natural source.

Pistacia vera L. hull, a the major byproduct of pistachio processing, is a source of functional compounds with antioxidant and antimicrobial activities. The extraction of these natural compounds from pistachio hulls and their use instead of synthetic chemicals has gained great attention. In this work, the phytochemical contents and antioxidant and antimicrobial activities of pistachio hull ethanolic (PVE) and aqueous (PVD) extracts obtained by microwave-assisted extraction (MAE) were investigated. Gallic acid (1.9 and 1.5 mg g-1 dw), quercetin (0.025 and 0.009 mg g-1 dw), total phenolic (23.3 and 14.7 mg GAE g-1 dw) and flavonoid (5.0 and 2.9 mg QE g-1 dw) contents and antioxidant activities (SC50 0.63 and 0.56 mg mL-1) of PVE and PVD extracts were determined, respectively. The extracts exhibited antimicrobial effects against Enterococcus faecalis, Staphylococcus aureus, Streptococcus uberis, Bacillus cereus, and Bacillus subtilis. Minimal inhibitory concentrations (MICs, 0.8-49.0 and 9.6-82.5 mg mL-1) and the minimal bactericidal concentrations (MBCs, 1.3-99.1 and 15.5-150.0 mg mL-1) of PVE and PVD extracts were determined, respectively. Kill curves revealed that PVE and PVD extracts could inhibit the growth of bacteria. It was shown that PVE and PVD extracts could represent a good economical source of functional and bioactive compounds.

Effects of fungal based bioactive compounds on human health: Review paper.

Since the first years of history, microbial fermentation products such as bread, wine, yogurt and vinegar have always been noteworthy regarding their nutritional and health effects. Similarly, mushrooms have been a valuable food product in point of both nutrition and medicine due to their rich chemical components. Alternatively, filamentous fungi, which can be easier to produce, play an active role in the synthesis of some bioactive compounds, which are also important for health, as well as being rich in protein content. Therefore, this review presents some important bioactive compounds (bioactive peptides, chitin/chitosan, ß-glucan, gamma-aminobutyric acid, L-carnitine, ergosterol and fructooligosaccharides) synthesized by fungal strains and their health benefits. In addition, potential probiotic- and prebiotic fungi were researched to determine their effects on gut microbiota. The current uses of fungal based bioactive compounds for cancer treatment were also discussed. The use of fungal strains in the food industry, especially to develop innovative food production, has been seen as promising microorganisms in obtaining healthy and nutritious food.

Fungal-based bioactive compounds have various health benefits.Prebiotic fungi play an active role in the regulation of gut microbiota.Anti-tumor effective fungal components will contribute to alternative medicine.Beta-glucan and chitin are the most promising fungal metabolites for cancer treatment.

Improvement in desulfurization of dibenzothiophene and dibenzothiophene sulfone by Paenibacillus strains using immobilization or nanoparticle coating.

AIMS: Biodesulfurization of fossil fuels is a promising technology for deep desulfurization. Previously, we have shown that Paenibacillus strains 32O-W and 32O-Y can desulfurize dibenzothiophene (DBT) and DBT sulfone (DBTS) effectively. In this work, improvements in DBT and DBTS desulfurization by these strains were investigated through immobilization and nanoparticle coating of cells. METHODS AND RESULTS: Paenibacillus strains 32O-W and 32O-Y immobilized in alginate gel beads or coated with Fe3 O4 magnetite nanoparticles were grown at various concentrations (0.1-2 mmol l-1 ) of DBT or DBTS for 96 h. The production of 2-hydroxybiphenyl (2-HBP) from the 4S pathway biotransformation of DBT or DBTS was measured. The highest amounts of 2-HBP production occurred at concentrations of 0.1 and 0.5 mmol l-1 . Compared to planktonic cultures maximum 2-HBP production increased by 54% for DBT and 90% for DBTS desulfurization with immobilized strains, and 44% for DBT and 66% for DBTS desulfurization by nanoparticle-coated strains. CONCLUSIONS: Nanoparticle-coated and immobilized cells may be of use in efforts to increase the efficiency of biodesulfurization. SIGNIFICANCE AND IMPACT OF THE STUDY: Alginate immobilization or nanoparticle coating of bacterial cells may be useful approaches for the enhancement of biodesulfurization for eventual use on an industrial scale.

Potential use of olive oil mill wastewater for bacterial cellulose production.

In this study, olive oil mill wastewater (OOMW), an important waste in the Mediterranean basin, was evaluated to produce bacterial cellulose (BC). For this purpose, the effects of different ratios of OOMW fractions (25-100%) and some additional nutrients (yeast extract, peptone and Hestrin-Schramm medium (HS) components) on BC productions were investigated. Unsupplemented OOMW medium (75% and 100%) yielded as much as BC obtained in HS medium (0.65 g/L), while enrichment of OOMW medium (%100) with yeast extract (5 g/L) and peptone (5 g/L) increased the amount of BC by 5.5 times, reaching to 5.33 g/L. In addition, produced BCs were characterized by FT-IR, TGA, XRD and SEM analyses. BC from OOMW medium (100% OOMW with supplementation) has a high thermal decomposition temperature (316.8°C), whereas it has lower crystallinity index (57%). According to the FT-IR analysis, it was observed that the components of OOMW might be absorbed by BCs. Thus, higher yield productions of BCs from OOMW media compared to BC obtained from HS medium indicate that olive oil industry wastes can be integrated into BC production for industrial applications.

Potential utilization of dairy industries by-products and wastes through microbial processes: A critical review.

The dairy industry generates excessive amounts of waste and by-products while it gives a wide range of dairy products. Alternative biotechnological uses of these wastes need to be determined to aerobic and anaerobic treatment systems due to their high chemical oxygen demand (COD) levels and rich nutrient (lactose, protein and fat) contents. This work presents a critical review on the fermentation-engineering aspects based on defining the effective use of dairy effluents in the production of various microbial products such as biofuel, enzyme, organic acid, polymer, biomass production, etc. In addition to microbial processes, techno-economic analyses to the integration of some microbial products into the biorefinery and feasibility of the related processes have been presented. Overall, the inclusion of dairy wastes into the designed microbial processes seems also promising for commercial approaches. Especially the digestion of dairy wastes with cow manure and/or different substrates will provide a positive net present value (NPV) and a payback period (PBP) less than 10 years to the plant in terms of biogas production.

Women's health anxiety and psychological wellbeing during the COVID-19 pandemic. A descriptive study.

BACKGROUND: The rapid spread of the novel coronavirus (COVID-19) outbreak has led to extraordinary measures taken worldwide and has led to serious psychological disorders. With the measures taken, the difficulties in women's daily lives are increasing exponentially. This situation has caused women to experience more mental health problems. OBJECTIVE: To identify the relationship between women's health anxiety and psychological wellbeing and the factors affecting these situations during the COVID-19 pandemic. DESIGN AND SETTING: Descriptive study conducted online among women living in Adana, Turkey. METHOD: This descriptive study was conducted among 623 married women between April 1 and April 20, 2020, using a SurveyMonkey online questionnaire. Data were collected using the link that was established. The questions comprised personal information, perceptions regarding the pandemic, the Health Anxiety Inventory (Short Form) and the Psychological Wellbeing Scale. RESULTS: The women who participated were found to have a high level of anxiety and a moderate level of psychological wellbeing. A positive, moderate-level relationship was found between the scales. CONCLUSIONS: The COVID-19 pandemic has had negative effects on both physical and psychological health. Support for women, to be provided within their holistic understanding of care, is of great importance for maintaining the psychological health of society.

Women's health anxiety and psychological wellbeing during the COVID-19 pandemic. A descriptive study

ABSTRACT BACKGROUND: The rapid spread of the novel coronavirus (COVID-19) outbreak has led to extraordinary measures taken worldwide and has led to serious psychological disorders. With the measures taken, the difficulties in women's daily lives are increasing exponentially. This situation has caused women to experience more mental health problems. OBJECTIVE: To identify the relationship between women's health anxiety and psychological wellbeing and the factors affecting these situations during the COVID-19 pandemic. DESIGN AND SETTING: Descriptive study conducted online among women living in Adana, Turkey. METHOD: This descriptive study was conducted among 623 married women between April 1 and April 20, 2020, using a SurveyMonkey online questionnaire. Data were collected using the link that was established. The questions comprised personal information, perceptions regarding the pandemic, the Health Anxiety Inventory (Short Form) and the Psychological Wellbeing Scale. RESULTS: The women who participated were found to have a high level of anxiety and a moderate level of psychological wellbeing. A positive, moderate-level relationship was found between the scales. CONCLUSIONS: The COVID-19 pandemic has had negative effects on both physical and psychological health. Support for women, to be provided within their holistic understanding of care, is of great importance for maintaining the psychological health of society.

Determination of the relationship between the fatigue and social support levels of cancer patients: a cross-sectional study.

BACKGROUND/AIMS: Cancer is seen as one of the most important health problems of our time. In the world and in our country, the most common death cause after cardiovascular diseases in the order of diseases that result in death is cancer. This descriptive, cross-sectional study is done in order to determine the relationship between fatigue and social support levels of cancer patients. METHODS: Research was carried out in Cukurova University Hospital in Adana, which is located in the south of Turkey, between December 2014 and December 2015. Data were collected by interview form prepared by the researcher, Piper Fatigue Scale and Social Support Patient Form. Data was analyzed by percentile, mean, independent t test, one-way analysis of variance, correlation and multiple regression. RESULTS: The average score of Social Support Patient Form is 131.1 ± 15.5, and Piper Fatigue Scale total score mean of the participants is 5.8 ± 2.4. A low level of negative correlation was found between the social support to cancer patients scale emotional support subdimension and the Piper Fatigue Scale affective subdimension. CONCLUSION: As a result of the research, it was seen that the level of fatigue of the participants had moderate and level of perceived social support was positive. The age, marital status, and education status of the participants do not affect the fatigue and social support levels.

Patient satisfaction on nursing care: the case of gynecology and obstetrics clinics / Satisfacción del paciente con el cuidado de enfermería: el caso de clínicas de ginecología y obstetricia / Satisfação do sobre cuidados de enfermagem: o caso daas clínicas ginecológicas e obstétricas

Abstract: Nursing care is an effective factor to allow the quality of health care services to be at a desired level. The ethical principles of justice and honesty are important for improving the quality of nursing care and patient satisfaction. This study determined satisfaction levels for nursing care of patients hospitalized in gynecology and obstetrics clinics of different types of hospitals providing health care services. This is a descriptive and cross-sectional study. The sample consisted of 420 female patients hospitalized in gynecology and obstetrics clinics. The data were collected using the "Patient Socio-demographics Form" and "Newcastle Nursing Care Satisfaction Scale." The nursing care satisfaction level of the participants according to hospital were as follows: Hospital A 70.68±15.51, Hospital B 70.65±15.17, Hospital C 65.41±16.48, Hospital D 71.39±14.66, and in total 67.2±16.13. There were statistically significant relationships between nursing satisfaction levels of participants based on the hospital, marital status, age, income level, and length of hospital stay. Satisfaction levels of the participants regarding nursing care were above average. While the satisfaction levels were similar based on hospitals, the satisfaction levels for the private hospital, training and research hospital, and university hospital were higher than that of the public hospital.

Resumen: El cuidado de enfermería es un factor efectivo en lograr la calidad al nivel deseado en el servicio del cuidado de salud. Los principios éticos de justicia y honestidad son importantes para mejorar la calidad del cuidado de enfermería y la satisfacción del paciente. El presente estudio determina los niveles de satisfacción del cuidado de enfermería de pacientes hospitalizados en clínicas de obstétrica y ginecología de diferentes tipos de hospitales que proporcionan servicios de cuidado de salud. Se trata de un estudio descriptivo y transversal. La muestra consistió de 420 mujeres pacientes hospitalizadas en clínicas de obstetricia y ginecología. Los datos se recolectaron usando el "Formulario sociodemográfico de pacientes" y la "Escala de satisfacción de cuidado de enfermería de Newcastle". Los niveles de satisfacción de cuidado de enfermería de los participantes de acuerdo a cada hospital fueron los siguientes: Hospital A 70.68±15.51, Hospital B 70.65±15.17, Hospital C 65.41±16.48, Hospital D 71.39±14.66, y el total 67.2±16. La relación entre los niveles de satisfacción del cuidado de enfermería de los pacientes basado en el hospital, estado marital, edad, nivel de salario y duración de estadía en el hospital fue estadísticamente significativa. Los niveles de satisfacción de los participantes respecto a los cuidados de enfermería se encontraron por encima del promedio. Mientras que los niveles de satisfacción eran similares basándose en hospitales, los niveles de satisfacción de los hospitales privados, de entrenamiento, investigación y universitarios fueron mayores que los de los hospitales públicos.

Cuidado de enfermagem é um fator importante para propiciar que a qualidade dos serviços de cuidados à saúde estejam em um nível desejado. Os princípios éticos de justiça e honestidade são importantes para melhorar a qualidade dos cuidados de enfermagem e a satisfação dos pacientes. Este estudo determinou os níveis de satisfação para cuidados de enfermagem de pacientes hospitalizadas em clínicas ginecológicas e obstétricas de diferentes tipos de hospitais que fornecem serviços de cuidados à saúde. Este é um estudo descritivo e de corte transversal. A amostra consistiu em 420 pacientes femininas hospitalizadas em clínicas ginecológicas e obstétricas. Os dados foram coletados utilizando-se o "Formulário Sócio-Demográfico do Paciente" e a "Escala de Satisfação com Cuidados de Enfermagem de Newcastle". O nível de satisfação dos participantes com cuidados de enfermagem de acordo com o hospital foram os seguintes: Hospital A 70.68±15.51, Hospital B 70.65±15.17, Hospital C 65.41±16.48, Hospital D 71.39±14.66, e no total 67.2±16.13. Houve relação estatisticamente significante entre níveis de satisfação com a enfermagem dos participantes com o hospital, estado civil, idade, nível de renda e tempo de hospitalização. Níveis de satisfação das participantes com respeito aos cuidados de enfermagem estiveram acima da média. Enquanto os níveis de satisfação foram similares entre os diferentes hospitais, os níveis de satisfação para os hospitais privados, hospitais de treinamento e pesquisa, e hospitais universitários foram mais altos que aqueles dos hospitais públicos.

In-situ wrapping of tin oxide nanoparticles by bacterial cellulose derived carbon nanofibers and its application as freestanding interlayer in lithium sulfide based lithium-sulfur batteries.

Lithium-Sulfur (Li-S) batteries are mostly known for their high energy density and cost-effectiveness. However, their intrinsic problems hinder their implementation into the marketplace. The most pronounced problems are the parasitic reactions which occur between lithium polysulfides species and lithium metal anode, the volume expansion of sulfur (80%) at the end of discharge and the safety issues which are linked with the use of lithium metal. Herein this work, two approaches are applied to prevent these effects; one approach is the use of Li2S as cathode material, instead of starting from sulfur powder, both to circumvent the volume expansion of sulfur taking place during discharge and to enable lithium-free anodes cell assembling (i.e. Si-Li2S or Sn-Li2S cell configurations). Second approach deals with the lithium anode protection by SnO2 containing freestanding pyrolyzed bacterial cellulose interlayers located between anode and cathode electrodes. Since bacterial celluloses are formed in the presence of SnO2 nanoparticles, the resulting structure enables intimate contact between carbon and SnO2 nanoparticles. By employing Li2S cathode and freestanding interlayer concurrently, 468 mAh g-1 discharge capacity is obtained at C/10 current density over 100 cycles.

The effect of music on pain and anxiety of women during labour on first time pregnancy: A study from Turkey.

OBJECTIVE: This study aims at analyzing the effect of music on pain and anxiety felt by women in labor during their first pregnancy. METHOD: When the pregnant women in the experimental group progressed into the active phase of the labor, they were made to listen to music in Acemasiran mode with earplugs for 3 h (20 min of listening with 10-min breaks). FINDINGS: It was observed that after the first-hour women indicated that their pain was statistically less in the experimental group. Trait anxiety scores of the women in labor were similar for experimental and control groups. Following the practice, state anxiety average scores became lower in favor of the experimental group and the correlation was statistically significant. CONCLUSION: In order to facilitate women's coping with labor pain and improve their wellbeing with the activity during the labor, musicotherapy, a non-pharmacological method, is an effective, simple and economical method.

Repeated batch fermentation of immobilized E. coli expressing Vitreoscilla hemoglobin for long-term use.

This study describes an efficient and reusable process for ethanol production from medium containing whey powder, using alginate immobilized ethanologenic E. coli strains either expressing (TS3) or not expressing (FBR5) Vitreoscilla hemoglobin. Reuseabilities of the FBR5 and TS3 strains were investigated regarding their ethanol production capacities over the course of 15 successive 96-h batch fermentations. The ethanol production was fairly stable over the entire duration of the experiment, with strain TS3 maintaining a substantial advantage over strain FBR5. Storage of both strains in 2 different solutions for up to 60 d resulted in only a modest loss of ethanol production, with strain TS3 consistently outperforming strain FBR5 by a substantial amount. Strains stored for 15 or 30 d maintained their abilities to produce ethanol without dimunition over the course of 8 successive batch fermentations; again strain TS3 maintained a substantial advantage over strain FBR5 throughout the entire experiment. Thus, immobilization is a useful strategy to maintain the advantage in ethanol productivity afforded by expression of Vitreoscilla hemoglobin over long periods of time and large numbers of repeated batch fermentations, including, as in this case, using media with food processing wastes as the carbon source.

Effective ethanol production from whey powder through immobilized E. coli expressing Vitreoscilla hemoglobin.

Ethanol production from whey powder was investigated by using free as well as alginate immobilized E. coli and E. coli expressing Vitreoscilla hemoglobin (VHb) in both shake flask and fermenter cultures. Media with varying levels of whey (lactose contents of 3%, 5%, 8% or 15%) and yeast extract (0.3% or 0.5%) were evaluated with fermentation times of 48-96 h. Immobilization and VHb expression resulted in higher ethanol production with all media; the increases ranged from 2% to 89% for immobilization and from 2% to 182% for VHb expression. It was determined that growth medium containing 8% lactose with 0.5% yeast extract yielded the highest ethanol production for free or immobilized strains, with or without VHb expression, in both shake flask and fermenter cultures. Immobilization with alginate was found to be a promising process for ethanol production by VHb-expressing ethanologenic E. coli.

Recent trends in bioethanol production from food processing byproducts.

The widespread use of corn starch and sugarcane as sources of sugar for the production of ethanol via fermentation may negatively impact the use of farmland for production of food. Thus, alternative sources of fermentable sugars, particularly from lignocellulosic sources, have been extensively investigated. Another source of fermentable sugars with substantial potential for ethanol production is the waste from the food growing and processing industry. Reviewed here is the use of waste from potato processing, molasses from processing of sugar beets into sugar, whey from cheese production, byproducts of rice and coffee bean processing, and other food processing wastes as sugar sources for fermentation to ethanol. Specific topics discussed include the organisms used for fermentation, strategies, such as co-culturing and cell immobilization, used to improve the fermentation process, and the use of genetic engineering to improve the performance of ethanol producing fermenters.

Use of organic acids for prevention and removal of Bacillus subtilis biofilms on food contact surfaces.

The efficacies of organic acid (citric, malic, and gallic acids) treatments at 1% and 2% concentrations on prevention and removal of Bacillus subtilis biofilms were investigated in this study. The analyses were conducted on microtitration plates and stainless steel coupons. The biofilm removal activities of these organic acids were compared with chlorine on both surfaces. The results showed that citric acid treatments were as powerful as chlorine treatments for prevention and removal of biofilms. The antibiofilm effects of malic acid treatments were higher than gallic acid and less than citric acid treatment. When the antibiofilm effects of these acids and chlorine on the two surfaces were compared, the prevention and removal of biofilms were measured higher on microtitration plates than those on stainless steel coupons. Higher reductions were obtained by increasing concentrations of sanitizers on 24-hour biofilm with 20-minute sanitizer treatments for removal of biofilms.

Efficient ethanol production from potato and corn processing industry waste using E. coli engineered to express Vitreoscilla haemoglobin.

Engineering of ethanologenic E. coli to express the haemoglobin (VHb) from the bacterium Vitreoscilla has been shown to enhance ethanol production by fermentation of pure sugars, sugars from hydrolysis of lignocellulose, components of whey, and sugars from wastewater produced during potato processing. Here, these studies were extended to see whether the same effect could be seen when a mixture of waste materials from processing of potatoes and corn into potato and corn chips were used as sugar sources. Consistent increases in ethanol production coincident with VHb expression were seen in shake flasks at both low aeration and high aeration conditions. The ethanol increases were due almost entirely to increases in the amount of ethanol produced per unit of cell mass. The VHb strategy for increasing fermentation to ethanol (and perhaps other valuable fermentation products) may be of general use, particularly regarding conversion of otherwise discarded materials into valuable commodities.

Improved ethanol production from cheese whey, whey powder, and sugar beet molasses by "Vitreoscilla hemoglobin expressing" Escherichia coli.

This work investigated the improvement of ethanol production by engineered ethanologenic Escherichia coli to express the hemoglobin from the bacterium Vitreoscilla (VHb). Ethanologenic E. coli strain FBR5 and FBR5 transformed with the VHb gene in two constructs (strains TS3 and TS4) were grown in cheese whey (CW) medium at small and large scales, at both high and low aeration, or with whey powder (WP) or sugar beet molasses hydrolysate (SBMH) media at large scale and low aeration. Culture pH, cell growth, VHb levels, and ethanol production were evaluated after 48 h. VHb expression in TS3 and TS4 enhanced their ethanol production in CW (21-419%), in WP (17-362%), or in SBMH (48-118%) media. This work extends the findings that "VHb technology" may be useful for improving the production of ethanol from waste and byproducts of various sources.

Redox-mediated interactions of VHb (Vitreoscilla haemoglobin) with OxyR: novel regulation of VHb biosynthesis under oxidative stress.

The bacterial haemoglobin from Vitreoscilla, VHb, displays several unusual properties that are unique among the globin family. When the gene encoding VHb, vgb, is expressed from its natural promoter in either Vitreoscilla or Escherichia coli, the level of VHb increases more than 50-fold under hypoxic conditions and decreases significantly during oxidative stress, suggesting similar functioning of the vgb promoter in both organisms. In the present study we show that expression of VHb in E. coli induced the antioxidant genes katG (catalase-peroxidase G) and sodA (superoxide dismutase A) and conferred significant protection from oxidative stress. In contrast, when vgb was expressed in an oxyR mutant of E. coli, VHb levels increased and the strain showed high sensitivity to oxidative stress without induction of antioxidant genes; this indicates the involvement of the oxidative stress regulator OxyR in mediating the protective effect of VHb under oxidative stress. A putative OxyR-binding site was identified within the vgb promoter and a gel-shift assay confirmed its interaction with oxidized OxyR, an interaction which was disrupted by the reduced form of the transcriptional activator Fnr (fumurate and nitrate reductase). This suggested that the redox state of OxyR and Fnr modulates their interaction with the vgb promoter. VHb associated with reduced OxyR in two-hybrid screen experiments and in vitro, converting it into an oxidized state in the presence of NADH, a condition where VHb is known to generate H2O2. These observations unveil a novel mechanism by which VHb may transmit signals to OxyR to autoregulate its own biosynthesis, simultaneously activating oxidative stress functions. The activation of OxyR via VHb, reported in the present paper for the first time, suggests the involvement of VHb in transcriptional control of many other genes as well.

Examination of knowledge about and nursing interventions for the care of patients in pain of nurses who work at Cukurova University medical faculty Balcali hospital.

Pain is a concept that goes back into distant history and is something that is faced by every person in different degrees and at different times in their lives. A definition of pain, adopted by the International Association for the Study of Pain and the American Pain Society, is: "an unpleasant sensory and emotional experience associated with actual or potential tissue damage, or described in terms of such damage." Although pain is one of the most common reasons for needing health care, it is not well understood and continues to be one of the most important health problems today. The pain experience is dynamic, and the nurse is responsible for understanding this. Nurses need to cooperate with the patient and other members of the health care team to be able to control pain. Nurses are ethically responsible for the management and easing of pain. This study was conducted to investigate what nurses know about the care of patients in pain and what nursing actions are used. There were 198 nurses working day shift at Cukurova University Balcali Hospital who were included. The data were collected using a questionnaire that described the nurses and measured the nurses' knowledge about care. The mean age of the nurses was 30.89 years, they had a mean 12.0 years of experience in the profession, 52.0% did not have experience with chronic pain, 42.4% stated that they frequently encountered patients in pain, 70.2% had received education about pain in school, 88.4% had not received education about pain outside of school and did not read about pain in journals, 88.9% used pharmacologic management, 85.4% evaluated patients' pain based on verbal statements, 96.5% knew the important points in the use of opioid analgesics, and 3% knew pain theory. As a result of this study, it is seen that nurses have inadequate knowledge about care of patients in pain and pain control methods. After evaluation of the conclusions, they will be used in education to increase the quality of the nursing care.