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1.
Indian J Pediatr ; 89(7): 682-691, 2022 07.
Artículo en Inglés | MEDLINE | ID: mdl-35239159

RESUMEN

OBJECTIVES: To determine the demographic, clinical, and genetic profile of Turkish Caucasian PCD cases. METHODS: Targeted next-generation sequencing (t-NGS) of 46 nuclear genes was performed in 21 unrelated PCD cases. Sanger sequencing confirmed of potentially disease-related variations, and genotype-phenotype correlations were evaluated. RESULTS: Disease-related variations were identified in eight different genes (CCDC39, CCDC40, CCDC151, DNAAF2, DNAAF4, DNAH11, HYDIN, RSPH4A) in 52.4% (11/21) of the cases. The frequency of variations for CCDC151, DNAH11, and DNAAF2 genes which were highly mutated genes in the cohort was 18% in 11 patients. Each of the remaining gene variations was detected once (9%) in different patients. The variants, p.R482fs*12 in CCDC151, p.E216* in DNAAF2, p.I317* in DNAAF4, p.L318P and p.R1865* in DNAH11, and p.N1505D and p.L1167P in HYDIN gene were identified as novel variations. Interestingly, varying phenotypic findings were identified even in patients with the same mutation, which once again confirmed that PCD has a high phenotypic heterogeneity and shows individual differences. CONCLUSION: This t-NGS panel is potentially helpful for exact and rapid identification of reported/novel PCD-disease-causing variants to establish the molecular diagnosis of ciliary diseases.


Asunto(s)
Síndrome de Kartagener , Estudios de Cohortes , Estudios de Asociación Genética , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Síndrome de Kartagener/diagnóstico , Síndrome de Kartagener/genética , Mutación
2.
Food Chem Toxicol ; 159: 112762, 2022 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-34896186

RESUMEN

Herbal products are being increasingly used all over the world for preventive and therapeutic purposes because of the belief of their safety. They have become an important part of health care system in many countries since they can easily be purchased in the health food stores or online. However, the lack of sufficient study on their efficacy and toxicity, inadequate controls of their availability, reduce their safety. Unlike conventional drugs, herbal products are not regulated for purity and potency. Herbal products contain substances which can induce or inhibit enzymes that take part in drug metabolism. Therefore the concurrent use of drugs with some medicinal plants can cause serious adverse effects and can also decrease the efficacy of the therapy. Particularly, drugs with narrow therapeutic index and plants which can affect drug metabolizing enzymes when used together, may lead to unpredictable adverse reactions. Impurities, contaminants and adulterants found in the herbal products, are the most common malpractises in herbal raw-material trade. In this review the unpredictable adverse effects of herbal products due to their possible interactions with drugs and also due to the adulteration and contamination with prohibited chemicals will be discussed in detail.


Asunto(s)
Contaminación de Medicamentos , Interacciones de Hierba-Droga , Preparaciones de Plantas , Animales , Humanos , Preparaciones de Plantas/efectos adversos , Preparaciones de Plantas/farmacocinética , Preparaciones de Plantas/toxicidad , Pruebas de Toxicidad
3.
Adv Food Nutr Res ; 89: 209-238, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31351526

RESUMEN

Diabetes mellitus, a chronic metabolic disease, characterized by elevated levels of blood glucose and insufficiency in production and action of insulin is the seventh leading cause of death worldwide. Numerous studies have shown that diabetes mellitus is associated with increased formation of free radicals and decrease in antioxidant potential. In the patients with diabetes mellitus, the levels of antioxidant parameters are found to decrease, hence in many studies phytochemicals which can exert antioxidant and free radical scavenging activities, are suggested to improve the insulin sensitivity. Several phytoactive compounds such as flavonoids, lignans, prophenylphenols, are also found to combat the complications of diabetes. This chapter mainly focuses on the relationship between diabetes mellitus and preventive roles of various phytochemicals on diabetes via their antioxidant properties.


Asunto(s)
Diabetes Mellitus/dietoterapia , Fitoquímicos/farmacología , Suplementos Dietéticos , Humanos , Estrés Oxidativo , Fitoquímicos/química , Fitoquímicos/clasificación
4.
Turk J Pharm Sci ; 15(2): 166-170, 2018 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-32454656

RESUMEN

Objectives: Diabetes, a heteregenous metabolic and chronic disease, is a growing health problem in most countries. It has been claimed that diabetes is associated with the increased formation of free radicals and decreased in antioxidant potential. Oxidative stress formed in diabetes may cause DNA damage in the tissues. Ursolic acid, a well-known pentacylic triterpene, is commonly used in traditional Chinese medicine due to its beneficial health effects such as antioxidant, anticancer, and antiulcer properties. The aim of this study was to investigate the effects of ursolic acid in the kidneys of Wistar albino rats with streptozotocin-induced diabetes. Materials and Methods: DNA damage was evaluated in the kidney cells of rats using alkaline comet assays. Oxidative stress parameters such as CAT, SOD, GR, and GSH-Px enzyme activities and total GSH and MDA levels were also evaluated. Results: Ursolic acid treatment was found to significantly decrease DNA damage, GR enzyme activities, and MDA levels, and significantly increase GSH levels and CAT, SOD and GSH-Px enzyme activities in diabetic rats. Conclusion: According to our results, it seems that ursolic acid may be beneficial against diabetes-induced renal damage.

5.
Food Chem Toxicol ; 110: 434-442, 2017 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-28923438

RESUMEN

It is known that diabetes causes some complications including alterations in lipid profile, hepatic enzyme levels but also it causes oxidative stress. Limonene, a major component of Citrus oils, has important health beneficial effects in lowering the level of oxidative stress due to its antioxidant activity. The aim of this study was to investigate the effects of D-limonene on streptozotocin (STZ)-induced diabetes in Wistar albino rats. For this purpose, DNA damage was evaluated by alkaline comet assay. Changes in the activities of catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GSHPx) and the levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), total glutathione (GSH), malondialdehyde (MDA), insulin, total bilirubin and BCA protein, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (GGT), high density lipoprotein (HDL), low density lipoprotein (LDL), total cholesterol and triglyceride were also evaluated. D-limonene treatment was found to significantly decrease DNA damage, GR enzyme activities and MDA levels and significantly increase GSH levels and CAT, SOD and GSH-Px enzyme activities and altered lipid and liver enzyme parameters in diabetic rats. According to our results, it seems that D-limonene might have a role in the prevention of the complication of diabetes in rats.


Asunto(s)
Citrus/química , Ciclohexenos/administración & dosificación , Diabetes Mellitus Experimental/tratamiento farmacológico , Extractos Vegetales/administración & dosificación , Terpenos/administración & dosificación , Animales , Aspartato Aminotransferasas/metabolismo , Catalasa/metabolismo , Ciclohexenos/química , Daño del ADN/efectos de los fármacos , Diabetes Mellitus Experimental/complicaciones , Diabetes Mellitus Experimental/genética , Diabetes Mellitus Experimental/metabolismo , Femenino , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Glutatión Reductasa/metabolismo , Humanos , Limoneno , Lipoproteínas HDL/metabolismo , Lipoproteínas LDL/metabolismo , Hígado/efectos de los fármacos , Hígado/enzimología , Hígado/metabolismo , Masculino , Malondialdehído/metabolismo , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/química , Ratas , Ratas Wistar , Superóxido Dismutasa/metabolismo , Terpenos/química
6.
Food Chem Toxicol ; 109(Pt 1): 210-217, 2017 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-28888732

RESUMEN

Phytochemicals derived from natural plants have been used commonly for the prevention and/or treatment of different diseases due to the belief of their safety. Many plant species synthesize toxic chemicals. New natural chemicals are being discovered but their toxic effects are unknown. Phytochemicals have been regarded as possible antioxidants. But on the other hand it is suggested that various phenolic antioxidants can display pro-oxidant properties at high doses. In this review, the role of some phytochemicals (epigallocathecin gallate, carvacrol, galangin, limonene, lycopene, naringin, puerarin, terpinene, thymol and ursolic acid) on the prevention of DNA damage will be discussed.


Asunto(s)
Daño del ADN/efectos de los fármacos , Fitoquímicos/farmacología , Extractos Vegetales/farmacología , Sustancias Protectoras/farmacología , Animales , Humanos
7.
Turk J Pharm Sci ; 14(2): 95-107, 2017 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-32454600

RESUMEN

Objectives: Phenolic compounds exhibit several health protective properties. Galangin, curcumin, pycnogenol, puerarin and ursolic acid are commonly used plant phenolics in folk medicine. The aim of our study was to evaluate the difference between neutral red uptake (NRU) and MTT assays using different plant phenolics (galangin, curcumin, pycnogenol, puerarin and ursolic acid) in healthy and cancer cells in different time periods. Materials and Methods: In this study, the cytotoxic effects of these phenolic compounds were investigated by NRU and MTT assays in healthy (V79, Chinese hamster fibroblast cell line) and cancer [human cervix epithelial adenocarcinoma cell line Henrietta Lacks (HeLa) and human mammary carcinoma cell line (BT-474)] in 18, 24 and 48 h incubation periods. Results: Our results demonstrated that galangin, curcumin, pycnogenol, puerarin and ursolic acid decreased cell viability of V79, HeLa and BT-474 cells in a dose-dependent manner in 18, 24 and 48 h incubation periods. However, the cell survival rate was much lower in 48 h incubation period. There was no difference between the results from NRU and MTT assays. Conclusion: To decide which incubation period and which cytotoxicity study to be used, the cytotoxicity mechanism of the compound must be known.

8.
Turk J Pharm Sci ; 14(3): 311-318, 2017 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-32454630

RESUMEN

It is well known that free oxygen radicals play an important role in the pathogenesis of several chronic disorders. Antioxidants are known as potential scavengers of reactive oxygen species that can protect biologic membranes against oxidative damage. Recent interest in phytochemicals has increased because of their protective effects against free oxygen radicals. Lycopene, which belongs to the carotenoid family, is the most effective singlet oxygen scavenger in vitro of all the carotenoids. Foods that contain lycopene and related supplements have been reported to prevent chronic diseases including cancer, asthma, and cardiovascular disorders. The aim of the article was to give a brief review of the antioxidant properties and beneficial health effects of lycopene.

9.
Drug Chem Toxicol ; 40(3): 256-262, 2017 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-27461151

RESUMEN

Phenolic compounds not only contribute to the sensory qualities of fruits and vegetables but also exhibit several health protective properties. Galangin, puerarin, and ursolic acid are commonly used plant phenolics in folk medicine. In this study, the antioxidant capacities of galangin, puerarin, and ursolic acid by the trolox equivalent antioxidant capacity (TEAC) assay and the cytotoxic effects by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in V79 cells were investigated. The genotoxic potentials of galangin, puerarin, and ursolic acid were evaluated by micronucleus (MN) and alkaline COMET assays in human lymphocytes and in V79 cells. Galangin, puerarin, and ursolic acid (10, 100, 500, 1000, 2000, 5000, 10 000, and 20 000 µM) were found to have antioxidant activities at the studied concentrations. IC50 values of galangin, puerarin, and ursolic acid in V79 cells were found to be 275.48 µM, 2503.712 µM, and 224.85 µM, respectively. Galangin, puerarin, and ursolic acid, at the all concentrations, have not exerted genotoxic effects and galangin, puerarin, and ursolic acid revealed a reduction in the frequency of MN and DNA damage induced by H2O2.


Asunto(s)
Antimutagênicos/farmacología , Antioxidantes/farmacología , Flavonoides/farmacología , Isoflavonas/farmacología , Triterpenos/farmacología , Animales , Antimutagênicos/toxicidad , Antioxidantes/toxicidad , Línea Celular , Supervivencia Celular/efectos de los fármacos , Ensayo Cometa , Cricetulus , Daño del ADN , Relación Dosis-Respuesta a Droga , Flavonoides/toxicidad , Humanos , Isoflavonas/toxicidad , Linfocitos/efectos de los fármacos , Linfocitos/patología , Micronúcleos con Defecto Cromosómico/inducido químicamente , Pruebas de Micronúcleos , Triterpenos/toxicidad
10.
Hum Exp Toxicol ; 35(8): 877-86, 2016 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-26429925

RESUMEN

Reactive oxygen species are believed to be involved in the development of sepsis. Plant-derived phenolic compounds are thought to be possible therapeutic agents against sepsis because of their antioxidant properties. Rosmarinic acid (RA) is a phenolic compound commonly found in various plants, which has many biological activities including antioxidant activity. The aim of this study was to investigate the effects of RA on sepsis-induced DNA damage in the lymphocytes and liver and kidney cells of Wistar albino rats by alkaline comet assay with and without formamidopyrimidine DNA glycosylase protein. The oxidative stress parameters such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and total glutathione (GSH) and malondialdehyde (MDA) levels in the liver and kidney tissues and an inflammatory cytokine, tumor necrosis factor α (TNF-α) level in plasma were also evaluated. It is found that DNA damage in the lymphocytes, livers, and kidneys of the RA-treated rats was significantly lower than that in the sepsis-induced rats. RA treatment also decreased the MDA levels and increased the GSH levels and SOD and GSH-Px activities in the livers and kidneys of the sepsis-induced rats. Plasma TNF-α level was found to be decreased in the RA-treated rats. It seems that RA might have a role in the attenuation of sepsis-induced oxidative damage not only by decreasing the DNA damage but also by increasing the antioxidant status and DNA repair capacity of the animals.


Asunto(s)
Antioxidantes/uso terapéutico , Cinamatos/uso terapéutico , Daño del ADN , Depsidos/uso terapéutico , Estrés Oxidativo/efectos de los fármacos , Sepsis/tratamiento farmacológico , Animales , Antioxidantes/administración & dosificación , Cinamatos/administración & dosificación , Ensayo Cometa , Citocinas/sangre , Citocinas/metabolismo , Depsidos/administración & dosificación , Riñón/efectos de los fármacos , Riñón/enzimología , Riñón/inmunología , Hígado/efectos de los fármacos , Hígado/enzimología , Hígado/inmunología , Masculino , Estrés Oxidativo/genética , Estrés Oxidativo/inmunología , Ratas Wistar , Sepsis/enzimología , Sepsis/genética , Sepsis/metabolismo
11.
Food Chem Toxicol ; 81: 160-170, 2015 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-25896273

RESUMEN

Phenolic compounds not only contribute to the sensory qualities of fruits and vegetables but also exhibit several health protective properties. Limonene and naringin are the most popular phenolics found in Citrus plants. In this study, we investigated the antioxidant capacities of limonene and naringin by the trolox equivalent antioxidant capacity (TEAC) assay and the cytotoxic effects by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in Chinese hamster fibroblast (V79) cells. The genotoxic potentials of limonene and naringin were evaluated by micronucleus (MN) and alkaline COMET assays in human lymphocytes and V79 cells. Limonene and naringin, were found to have antioxidant activities at concentrations of 2-2000 µM and 5-2000 µM respectively. IC50 values of limonene and naringin were found to be 1265 µM and 9026 µM, respectively. Limonene at the concentrations below 10,000 µM and naringin at the all concentrations studied, have not exerted genotoxic effects in lymphocytes and in V79 cells. Limonene and naringin at all concentrations revealed a reduction in the frequency of MN and DNA damage induced by H2O2.


Asunto(s)
Antioxidantes/farmacología , Citrus/química , Ciclohexenos/farmacología , Flavanonas/farmacología , Fenoles/farmacología , Terpenos/farmacología , Animales , Línea Celular , Ensayo Cometa , Cricetinae , Daño del ADN/efectos de los fármacos , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Humanos , Peróxido de Hidrógeno/toxicidad , Limoneno , Linfocitos/efectos de los fármacos , Linfocitos/metabolismo , Pruebas de Micronúcleos
12.
J Anesth ; 29(3): 360-366, 2015 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-25376969

RESUMEN

BACKGROUND: Preeclampsia is characterized by increased extracellular fluid which manifests as generalized edema due to endothelial injury and subsequent capillary leak. Therefore, preeclampsia may lead to increased skin to subarachnoid distance (SSD) which may influence daily clinical practice in this particular gravid population. METHODS: Age- and height-matched gravidas with and without preeclampsia were enrolled prospectively at an allocation ratio of 1:4. Spinal anesthesia (SA) was performed in a sitting position by a mid-line approach at the L3-L4 interspace using a 25-gauge Quincke spinal needle. An internal pilot study was performed to determine the sample size. When the protocol violations were excluded, 146 gravidas were included in the study (25 preeclamptics and 121 normotensive controls) for final analysis. RESULTS: On average, SSD was 0.89 cm greater in preeclamptics compared to normotensive controls. Mean values of the SSD in preeclamptic and normotensive control group patients at the L3-L4 interspace were 6.187 ± 0.967 and 5.301 ± 0.834 cm, respectively. SSD was significantly correlated with body weight and body mass index (BMI). The regression formula for the estimation of SSD in preeclamptic gravidas with BMI during SA was SSD = 3.696 + 0.075×BMI. The regression formula for the estimation of SSD in the normotensive control group with BMI during SA was SSD = 3.144 + 0.067×BMI - 0.0001×BMI×BMI. CONCLUSION: Knowing that the SSD is increased in preeclamptics compared to normotensive gravidas may be of value in terms of selecting needle, and providing safe and comfortable anesthesia.


Asunto(s)
Anestesia Raquidea/métodos , Preeclampsia/fisiopatología , Piel/metabolismo , Adulto , Presión Sanguínea , Índice de Masa Corporal , Peso Corporal , Estudios de Cohortes , Femenino , Humanos , Agujas , Proyectos Piloto , Embarazo , Estudios Prospectivos , Adulto Joven
13.
Hippokratia ; 19(2): 125-30, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-27418760

RESUMEN

OBJECTIVE: The objective of this study was to determine the efficacy of the exercises administered to stroke patients with the balance trainer (BALANCE-trainer, art.nr. 07001-001(TM)) on balance, level of independence and ambulation parameters. MATERIAL AND METHOD: Fifty patients with hemiplegia were randomized into either study group or control group. Patients in the control group received 30 sessions of conventional rehabilitation program and patients in the study group were trained with balance trainer in addition to conventional rehabilitation program. Balance level and postural control were evaluated with Berg Balance Scale (BBS) and Timed-Up and Go Test (TUG). Their functional statuses were evaluated using Functional Independence Measure (FIM). Evaluations were repeated following the six-week rehabilitation program. RESULTS: Of the 50 participants, 19 were women (38%) and 31 were men (62%). The mean age was 57.1 ± 9.2 years. The time that elapsed after stroke was 87.3 ± 26.3 days. Statistically significant improvements were noted in BBS, TUG and FIM in intra-group evaluations for both groups. Statistically significant improvements were documented in BBS and TUG levels for inter-group evaluation (respectively p =0.038, p =0.025), while the difference in FIM levels was not statistically significant (p >0.05). CONCLUSION: Positive impact of balance trainer on balance and postural control was demonstrated in stroke patients in the current study. Hippokratia 2015; 19 (2):125-130.

14.
Environ Toxicol Pharmacol ; 38(3): 774-82, 2014 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-25305738

RESUMEN

Oxidative stress has an important role in the development of sepsis-induced multiorgan failure. Ferulic acid (FA), a well-established natural antioxidant, has several pharmacological activities including anti-inflammatory, anticancer and hepatoprotective. This study aimed to investigate the effects of FA on sepsis-induced oxidative damage in Wistar albino rats. Sepsis-induced DNA damage in the lymphocytes, liver and kidney cells of rats were evaluated by comet assay with and without formamidopyrimidine DNA glycosylase (Fpg). The oxidative stress parameters such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and total glutathione (GSH) and malondialdehyde (MDA) levels were also measured. It is found that DNA damage in sepsis+FA-treated group was significantly lower than the sepsis group. FA treatment also decreased the MDA levels and increased the GSH levels and SOD and GSH-Px activities in the sepsis-induced rats. It seems that FA might have ameliorative effects against sepsis-induced oxidative damage.


Asunto(s)
Antioxidantes/administración & dosificación , Ácidos Cumáricos/administración & dosificación , Daño del ADN/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Sepsis/patología , Animales , Antioxidantes/farmacología , Ácidos Cumáricos/farmacología , Regulación de la Expresión Génica/efectos de los fármacos , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Riñón/metabolismo , Hígado/metabolismo , Linfocitos/metabolismo , Malondialdehído/metabolismo , Ratas , Ratas Wistar , Sepsis/metabolismo , Superóxido Dismutasa/metabolismo
15.
Phytother Res ; 28(11): 1692-700, 2014 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-24919414

RESUMEN

The aim of this study was to evaluate the protective effects of Pycnogenol® (Pyc), a complex plant extract from the bark of French maritime pine, on oxidative stress parameters (superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities and total glutathione (GSH) and malondialdehyde (MDA) levels), an inflammatory cytokine (tumor necrosis factor alpha (TNF-α) level) and also DNA damage in Wistar albino rats. Rats were treated with 100 mg/kg intraperitonally Pyc following the induction of sepsis by cecal ligation and puncture. The decreases in MDA levels and increases in GSH levels, and SOD and GPx activities were observed in the livers and kidneys of Pyc-treated septic rats. Plasma TNF-α level was found to be decreased in the Pyc-treated septic rats. In the lymphocytes, kidney, and liver tissue cells of the sepsis-induced rats, Pyc treatment significantly decreased the DNA damage and oxidative base damage using standard alkaline assay and formamidopyrimidine DNA glycosylase-modified comet assay, respectively. In conclusion, Pyc treatment might have a role in the prevention of sepsis-induced oxidative damage not only by decreasing DNA damage but also increasing the antioxidant status and DNA repair capacity in rats.


Asunto(s)
Daño del ADN/efectos de los fármacos , Flavonoides/farmacología , Estrés Oxidativo/efectos de los fármacos , Sepsis/patología , Animales , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Riñón/efectos de los fármacos , Riñón/metabolismo , Hígado/efectos de los fármacos , Hígado/metabolismo , Malondialdehído/metabolismo , Pinus/química , Extractos Vegetales , Ratas Wistar , Superóxido Dismutasa/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo
16.
Eur J Phys Rehabil Med ; 49(6): 815-22, 2013 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-24285025

RESUMEN

BACKGROUND: The caregivers of children with cerebral palsy (CP) should overcome the difficulties and complications arising from their children's impairments. It may be stressful for the caregivers when the balance between these inevitable demands and their own social needs impairs. Therefore, the primary caregiver, may experience several psycho-social problems. AIM: To compare the quality of life (QoL), mental health and burnout of caregivers of patients with CP and healthy controls. The effects of the functional limitations of children with CP on the QoL, mental health and burnout of caregivers have also been evaluated. DESIGN: Cross-sectional, comparative. SETTING: Outpatient. POPULATION: One hundred and forty-three caregivers of children with CP and 60 caregivers of typically developing children were recruited for the study. MATERIALS AND METHODS: The inventories related to QoL, mental health and burnout of the caregivers for both groups were compared. The correlations between functional limitations of the children with CP and QoL, mental health and burnout of their caregivers have been analyzed. RESULTS: Caregivers in CP group had poorer QoL, worser mental health, and higher burnout levels compared to the controls. The functional limitations of the children with CP were correlated with impairment of QoL and depression scores of their caregivers, but not with the anxiety or burnout scores. CONCLUSION: Having a disabled child has interference on caregivers' QoL and mental health and increases the burnout. Furthermore, as the functional impairment level of the child increases, the interference does too. Clinical rehabilitation impact. Health professionals working in this area should also consider the mental health and the QoL of caregivers and should develop interventions that support and nurture the family as a whole.


Asunto(s)
Cuidadores/psicología , Parálisis Cerebral/fisiopatología , Niños con Discapacidad , Salud de la Familia , Padres/psicología , Calidad de Vida/psicología , Estrés Psicológico/etiología , Adolescente , Adulto , Ansiedad/etiología , Ansiedad/psicología , Parálisis Cerebral/clasificación , Niño , Preescolar , Estudios Transversales , Depresión/etiología , Depresión/psicología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Índice de Severidad de la Enfermedad , Apoyo Social , Factores Socioeconómicos , Estrés Psicológico/psicología , Turquia , Adulto Joven
17.
Hum Exp Toxicol ; 32(10): 1048-57, 2013 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-23155200

RESUMEN

Sepsis, often initiated by an infection, is a state of disrupted inflammatory homeostasis. There is increasing evidence that oxidative stress has an important role in the development of sepsis-induced multiorgan failure. Resveratrol (RV) is a polyphenolic compound found in the skin of red fruits, such as mulberries and red grapes, and in peanuts. RV has been reported to have an antioxidant, antiproliferative, and anti-inflammatory properties in various models. It has also been found to inhibit the proliferation of a variety of human cancer cell lines, including breast, prostate, colon, pancreatic, and thyroid. This study has been undertaken to assess the role of RV on the sepsis-induced oxidative DNA damage in the lymphocytes of Wistar albino rats by the standard and formamidopyrimidine DNA glycosylase (Fpg)-modified comet assays. The parameters of tail length, tail intensity, and tail moment were evaluated for the determination of DNA damage. According to the study, the DNA damage was found to be significantly higher in the sepsis-induced rats when compared with the control rats (p < 0.05). The parameters were significantly decreased in the RV-treated sepsis-induced group when compared with the sepsis-induced group. The parameters in the sepsis-induced rats were found to be significantly higher in the Fpg-modified comet assay when compared with the standard comet assay (p < 0.05), and RV treatment decreases the DNA damage in the sepsis-induced rats, suggesting that the oxidative stress is likely to be responsible for DNA damage and RV might have a role in the prevention of sepsis-induced oxidative DNA damage.


Asunto(s)
Antimutagênicos/farmacología , Daño del ADN/efectos de los fármacos , Sepsis/complicaciones , Estilbenos/farmacología , Animales , Ensayo Cometa , ADN-Formamidopirimidina Glicosilasa , Linfocitos/efectos de los fármacos , Linfocitos/metabolismo , Ratas , Ratas Wistar , Resveratrol
18.
J Obstet Gynaecol ; 32(2): 120-3, 2012 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-22296418

RESUMEN

Cyclooxygenase inhibitors (CI) which contained risks to fetal health were one of the most effective tocolytics. In order to indirectly investigate the effects of CI in human ductus arteriosus, immunohistochemical staining for cyclooxygenase-1 (COX1) and cyclooxygenase-2 (COX2) was evaluated in post-mortem fetuses with gestational ages between 24 and 34 weeks. Neither COX1 nor COX2 staining was related to gestational age. COX1 and COX2 staining in the vessel walls were not related to each other. COX1 staining in the endothelium, inner media and outer media were positively correlated with each other (COX1 endothelium vs IM staining Spearman's rho statistic [rs] = 0.721, p = 0.001; COX1 endothelium vs OM staining [rs] = 0.634, p = 0.004; COX1 IM vs OM staining [rs] = 0.931, p = 0.001). COX2 staining of endothelium was not correlated with either IM or OM staining. In conclusion, COX2 staining in the post-mortem specimens of human ductus arteriosus between 24 and 34 weeks is weak and limited to the endothelium.


Asunto(s)
Ciclooxigenasa 1/metabolismo , Ciclooxigenasa 2/metabolismo , Conducto Arterial/enzimología , Edad Gestacional , Endotelio Vascular/enzimología , Femenino , Humanos , Inmunohistoquímica , Embarazo
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