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1.
Bioact Mater ; 20: 42-52, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-35633873

RESUMEN

Soft tissue integration is one major difficulty in the wide applications of metal materials in soft tissue-related areas. The inevitable inflammatory response and subsequent fibrous reaction toward the metal implant is one key response for metal implant-soft tissue integration. It is of great importance to modulate this inflammatory-fibrous response, which is mainly mediated by the multidirectional interaction between fibroblasts and macrophages. In this study, macrophages are induced to generate M1 and M2 macrophage immune microenvironments. Their cytokine profiles have been proven to have potentially multi-regulatory effects on fibroblasts. The multi-reparative effects of soft tissue cells (human gingival fibroblasts) cultured on metal material (titanium alloy disks) in M1 and M2 immune microenvironments are then dissected. Fibroblasts in the M1 immune microenvironment tend to aggravate the inflammatory response in a pro-inflammatory positive feedback loop, while M2 immune microenvironment enhances multiple functions of fibroblasts in soft tissue integration, including soft tissue regeneration, cell adhesion on materials, and contraction to immobilize soft tissue. Enlighted by the close interaction between macrophages and fibroblasts, we propose the concept of an "inflammatory-fibrous complex" to disclose possible methods of precisely and effectively modulating inflammatory and fibrous responses, thus advancing the development of metal soft tissue materials.

2.
Oxid Med Cell Longev ; 2022: 4564471, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35308167

RESUMEN

The polarization of microglia is recognized as a crucial factor in reducing neuroinflammation and promoting hematoma clearance after intracerebral hemorrhage (ICH). Previous studies have revealed that redox components participate in the regulation of microglial polarization. Recently, the novel Nrf2 activator omaveloxolone (Omav) has been validated to improve neurological function in patients with neurodegenerative disorders by regulating antioxidant responses. In this study, we examined the efficacy of Omav in ICH. Omav significantly promoted Nrf2 nuclear accumulation and the expression of HO-1 and NQO1 in BV2 cells. In addition, both in vitro and in vivo experiments showed that Omav treatment inhibited M1-like activation and promoted the activation of the M2-like microglial phenotype. Omav inhibited OxyHb-induced ROS generation and preserved the function of mitochondria in BV2 cells. Intraperitoneal administration of Omav improved sensorimotor function in the ICH mouse model. Importantly, these effects were blocked by pretreatment with ML385, a selective inhibitor of Nrf2. Collectively, Omav modulated microglial polarization by activating Nrf2 and inhibiting ROS generation in ICH models, suggesting that it might be a promising drug candidate for the treatment of ICH.


Asunto(s)
Lesiones Encefálicas , Microglía , Animales , Lesiones Encefálicas/tratamiento farmacológico , Hemorragia Cerebral/complicaciones , Hemorragia Cerebral/tratamiento farmacológico , Hemorragia Cerebral/metabolismo , Humanos , Ratones , Microglía/metabolismo , Factor 2 Relacionado con NF-E2/metabolismo , Fenotipo , Triterpenos
3.
Sci Data ; 9(1): 465, 2022 Aug 02.
Artículo en Inglés | MEDLINE | ID: mdl-35918334

RESUMEN

The dataset of simultaneous 64-channel electroencephalography (EEG) and high-speed eye-tracking (ET) recordings was collected from 31 professional athletes and 43 college students during alertness behavior task (ABT) and concentration cognitive task (CCT). The CCT experiment lasting 1-2 hours included five sessions for groups of the Shooting, Archery and Modern Pentathlon elite athletes and the controls. Concentration targets included shooting target and combination target with or without 24 different directions of visual distractors and 2 types of music distractors. Meditation and Schulte Grid trainings were done as interventions. Analysis of the dataset aimed to extract effective biological markers of eye movement and EEG that can assess the concentration level of talented athletes compared with same-aged controls. Moreover, this dataset is useful for the research of related visual brain-computer interfaces.


Asunto(s)
Electroencefalografía , Tecnología de Seguimiento Ocular , Atletas , Atención , Movimientos Oculares , Humanos
4.
Brain Behav ; : e2693, 2022 Jul 11.
Artículo en Inglés | MEDLINE | ID: mdl-35816591

RESUMEN

BACKGROUND AND PURPOSE: Previous studies have revealed abnormal regional homogeneity (ReHo) in individuals with autism spectrum disorder (ASD); however, there is little consistency across the findings within these studies, partly due to small sample size and great heterogeneity among participants between studies. Additionally, few studies have explored the association between ReHo aberrance and clinical symptoms in individuals with ASD. METHODS: Forty-eight adolescents with high-functioning ASD and 63 group-matched typically developing (TD) controls received functional magnetic resonance imaging at rest. Group-level analysis was performed to detect differences in ReHo between ASD and TD. Evaluation of symptom severity in individuals with ASD was based on the Autism Behavior Checklist (ABC). Voxel-wise correlation analysis was undergone to examine the correlations between the symptom severity and ReHo map in individuals with ASD within brain areas with ReHo abnormalities. RESULTS: Compared with the TD controls, individuals with ASD exhibited increased ReHo in the bilateral anterior cingulate cortex, left caudate, right posterior cerebellum (cerebellar tonsil), and bilateral brainstem and decreased ReHo in the left precentral gyrus, left inferior parietal lobule, bilateral postcentral gyrus, and right anterior cerebellum (culmen). The correlation analysis indicated that the ReHo value in the brainstem was negatively associated with the ABC total scores and the scores of Relating factor, respectively. CONCLUSIONS: Our findings indicated that widespread ReHo abnormalities occurred in ASD, shedding light on the underlying neurobiology of pathogenesis and symptomatology of ASD.

5.
Anim Sci J ; 93(1): e13751, 2022 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-35831959

RESUMEN

In this study, we aimed to assess the effects of probiotic administration on the digestibility characteristics and growth performance of finishing beef cattle fed a total mixed ration (TMR) containing different levels of corn stover. One hundred and sixty Simmental × Continental crossbred bulls were randomly allocated to two animal houses (80 bulls each) and randomly assigned four TMR differing in the level of corn stover-high (HCT) and low (LCT)-with or without probiotics in each animal house. Feeding HCT supplemented with probiotics increased (P < 0.05) the apparent digestibility of crude protein (CP), ether extract (EE), neutral detergent fiber (NDF), and acid detergent fiber (ADF). Regardless of probiotic supplementation, the nitrogen intake and fecal nitrogen levels of animals fed HCT were lower than those fed LCT (P < 0.05). Additionally, feeding probiotics increased (P < 0.05) the efficiency of ruminal fermentation, final body weight, and average daily gain (ADG) of animals, with this effect being stronger in animals fed HCT. In conclusion, supplementing probiotics with HCT has a positive effect on the growth of finishing beef cattle, thereby providing economic benefits.


Asunto(s)
Probióticos , Zea mays , Alimentación Animal/análisis , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Bovinos , Detergentes/metabolismo , Detergentes/farmacología , Dieta/veterinaria , Digestión , Fermentación , Masculino , Nitrógeno/metabolismo , Rumen/metabolismo
6.
Artículo en Inglés | MEDLINE | ID: mdl-35839436

RESUMEN

Freshwater scarcity is a critical challenge threatening human survival especially due to poverty and arid and off-grid regions. Sorption-based atmospheric water harvesting (AWH) has emerged as a promising strategy for clean water production. However, most of the high-capacity sorbents are limited by the poor sorption/desorption kinetics and uncontrollable liquid leakage problem. Inspired by the plant transpiration process, we develop an environmentally friendly LiCl@pollen cell-polypyrrole (LiCl@PC-PPy) composite sorbent by confining the LiCl hygroscopic agent in the cages of the PC-PPy. The composite sorbent exhibits much improved sorption/desorption kinetics owing to the hydrophilicity of the hierarchical porous structure of the pollen cells, which provides abundant water sorption active sites and diffusion pathways and forms a concave meniscus on cell skeletons to maximize the thermal utilization efficiency. Moreover, the big cavities of the PC-PPy cages can serve as a water reservoir to reduce liquid leakage. As a result, the sorbent can capture atmospheric water to 85% of its own weight under 60% relative humidity (RH) within 2 h and rapidly release the water within 1 h under weak light irradiation of 0.8 sun. As a proof-of-concept demonstration, the fabricated AWH device can absorb 1.55 gwater/gsorbent at night and collect 1.53 gwater/gsorbent of water in 1-day outdoor operation, and the collected water can meet the drinking water standards defined by the World Health Organization (WHO) and Environmental Protection Agency (EPA).

7.
Artículo en Inglés | MEDLINE | ID: mdl-35840703

RESUMEN

PURPOSE: For some surgical conditionns and scientific questions, the "real world" effectiveness of surgical patient care may be better explored using a multi-institutional time-bound observational cohort assessment approach (termed a "snapshot audit") than by retrospective review of administrative datasets or by prospective randomized control trials. We discuss when this might be the case, and present the key features of developing, deploying, and assessing snapshot audit outcomes data. METHODS: A narrative review of snapshot audit methodology was generated using the Scale for the Assessment of Narrative Review Articles (SANRA) guideline. Manuscripts were selected from domains including: audit design and deployment, statistical analysis, surgical therapy and technique, surgical outcomes, diagnostic testing, critical care management, concomitant non-surgical disease, implementation science, and guideline compliance. RESULTS: Snapshot audits all conform to a similar structure: being time-bound, non-interventional, and multi-institutional. A successful diverse steering committee will leverage expertise that includes clinical care and data science, coupled with librarian services. Pre-published protocols (with specified aims and analyses) greatly helps site recruitment. Mentored trainee involvement at collaborating sites should be encouraged through manuscript contributorship. Current funding principally flows from medical professional organizations. CONCLUSION: The snapshot audit approach to assessing current care provides insights into care delivery, outcomes, and guideline compliance while generating testable hypotheses.

8.
iScience ; 25(7): 104601, 2022 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-35789837

RESUMEN

Flexible polymer dielectrics which can function well at elevated temperatures continue to be significant in harsh condition energy storage. However, state-of-the-art high-temperature polymers traditionally designed with conjugated structures for better thermal stability have compromised bandgaps and charge injection barriers. Here, we demonstrate a self-assembled polyvinyl alcohol (PVA)/montmorillonite (MMT) coating to impede charge carriers injecting into the polyimide (PI) polymer film. The anisotropic conductivity of the 2D nanolayered coating further dissipates the energy of charges through tortuous injection pathways. With the coating, high field pre-breakdown conduction measurement and space-charge profiling of PI films reveal a clear shifting of the dominant mode of conduction from the bulk-limited hopping to Schottky-injection limited conduction. The coating thus imparts PI films with a significantly suppressed electrical conduction (∼10×), and substantially improved discharge efficiency (7×) and energy density (2.7×) at 150°C. The facile and scalable flow-induced fabrication unleash enormous applications for harsh condition electrification.

9.
Front Neurol ; 13: 892480, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35832175

RESUMEN

Sepsis-associated encephalopathy (SAE), the most popular cause of coma in the intensive care unit (ICU), is the diffuse cerebral damage caused by the septic challenge. SAE is closely related to high mortality and extended cognitive impairment in patients in septic shock. At present, many studies have demonstrated that SAE might be mainly associated with blood-brain barrier damage, abnormal neurotransmitter secretion, oxidative stress, and neuroimmune dysfunction. Nevertheless, the precise mechanism which initiates SAE and contributes to the long-term cognitive impairment remains largely unknown. Recently, a growing body of evidence has indicated that there is close crosstalk between SAE and peripheral immunity. The excessive migration of peripheral immune cells to the brain, the activation of glia, and resulting dysfunction of the central immune system are the main causes of septic nerve damage. This study reviews the update on the pathogenesis of septic encephalopathy, focusing on the over-activation of immune cells in the central nervous system (CNS) and the "neurocentral-endocrine-immune" networks in the development of SAE, aiming to further understand the potential mechanism of SAE and provide new targets for diagnosis and management of septic complications.

10.
Sensors (Basel) ; 22(13)2022 Jul 04.
Artículo en Inglés | MEDLINE | ID: mdl-35808530

RESUMEN

As an advanced technology, simultaneous wireless information and power transfer (SWIPT), combined with the internet of things (IoT) devices, can effectively extend the online cycle of the terminal. To cope with the fluctuation of energy harvesting by the hybrid access points (H-AP), the energy cooperation base station is introduced to realize the sharing of renewable energy. In this paper, we study the SWIPT-enabled IoT networks with cooperation. Our goal is to maximize the energy efficiency of the system, and at the same time, we need to meet the energy harvesting constraints, user quality of service (QoS) constraints and transmission power constraints. We jointly solve the power allocation, time switching and energy cooperation problems. Because this problem is a nonlinear programming problem, it is difficult to solve directly, so we use the alternating variable method, the iterative algorithm is used to solve the power allocation and time switching problem, and the matching algorithm is used to solve the energy cooperation problem. Simulation results show that the proposed algorithm has obvious advantages in energy efficiency performance compared with the comparison algorithm. At the same time, it is also proved that the introduction of energy cooperation technology can effectively reduce system energy consumption and improve system energy efficiency.

11.
Open Life Sci ; 17(1): 744-755, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35891967

RESUMEN

We investigated the role of the interaction between the Notch and Toll-like receptor 4 (TLR4) pathways in septic myocardial injury. The sepsis model was induced in rats with lipopolysaccharide (LPS). Rats were divided into control, LPS, LPS + TAK242 ((6R)-6-[N-(2-chloro-4-fluorophenyl)sulfamoyl]cyclohex-1-ene-1-carboxylate) and LPS + DAPT (N-[N-(3,5-difluorophenacetyl)-l-alanyl]-s-phenylglycinetbutylester) groups. Heart function was evaluated with a Cardiac Doppler ultrasound. Myocardial morphological changes were detected by hematoxylin-eosin staining (H&E). Apoptosis was assessed by a TUNEL assay. The mRNA and protein levels were detected with real-time PCR, Western blot, and immunohistochemistry analysis. We found that heart function in the LPS + TAK242 group was significantly improved, but not in the LPS + DAPT group. LPS + TAK242 had a lower level of degeneration and necrosis of cardiomyocytes and inflammatory cell infiltration, as well as lower apoptosis and caspase-3 expression than the LPS group. Compared with the LPS group, the inflammatory cell infiltration was reduced in the LPS + DAPT group, while the degeneration and necrosis of cardiomyocytes were not obviously improved. Additionally, the expression levels of tumor necrosis factor-α and Interleukin-6, the protein contents of Notch intracellular domain and Hes1, and the P65 nuclear factor kappa-B (NF-κB) to P-P65 NF-κB ratio in LPS + TAK242 group and LPS + DAPT group were significantly lower than those in LPS group. Conclusively, the interaction between TLR4 and Notch signaling pathways enhances the inflammatory response in the septic heart by activating NF-κB. Blocking the TLR4 pathway with TAK242 can improve heart dysfunction and myocardial damage in sepsis, while blocking the Notch pathway with DAPT cannot effectively prevent heart dysfunction and myocardial damage in sepsis.

12.
Nat Mater ; 2022 Jul 25.
Artículo en Inglés | MEDLINE | ID: mdl-35879439

RESUMEN

Formation of epitaxial heterostructures via post-growth self-assembly is important in the design and preparation of functional hybrid systems combining unique properties of the constituents. This is particularly attractive for the construction of metal halide perovskite heterostructures, since their conventional solution synthesis usually leads to non-uniformity in composition, crystal phase and dimensionality. Herein, we demonstrate that a series of two-dimensional and three-dimensional perovskites of different composition and crystal phase can form epitaxial heterostructures through a ligand-assisted welding process at room temperature. Using the CsPbBr3/PEA2PbBr4 heterostructure as a demonstration, in addition to the effective charge and energy transfer across the epitaxial interface, localized lattice strain was observed at the interface, which was extended to the top layer of the two-dimensional perovskite, leading to multiple new sub-bandgap emissions at low temperature. Given the versatility of our strategy, unlimited hybrid systems are anticipated, yielding composition-, interface- and/or orientation-dependent properties.

13.
Stem Cell Res Ther ; 13(1): 321, 2022 07 16.
Artículo en Inglés | MEDLINE | ID: mdl-35842684

RESUMEN

BACKGROUND: The novel coronavirus is still mutating, and the pandemic continues. Meanwhile, many COVID-19 survivors have residual postinfection clinical manifestations. Human umbilical cord mesenchymal stem cells (hUC-MSCs) have been shown to be effective in the early stages of COVID-19. OBJECTIVES: The aim of this study was to investigate long-term safety and efficacy of treatment in patients with severe COVID-19 patients who had received hUC-MSCs therapy. METHODS: Twenty-five discharged patients who had severe COVID-19 (including the standard treatment group and the standard treatment plus hUC-MSCs group) were enrolled in a 1-year follow-up. The assessment considered adverse effects (including effects on liver and kidney function, coagulation, ECG, tumor marker, and so on), pulmonary function, St George's Respiratory Questionnaire (SGRQ), postinfection sequelae and serum concentration of Krebs von den Lungen-6 (KL-6), malondialdehyde (MDA), H2S, carnitine, and N-6 long-chain polyunsaturated fatty acids (N-6 LC-PUFAs). MEASUREMENTS AND MAIN RESULTS: Pulmonary ventilation function had significantly improved at the 1-year follow-up in both the hUC-MSCs group and the control group compared with the 3-month follow-up (P < 0.01). Fatigue (60% [15/25]) remained the most common symptom at the 1-year follow-up. The rate of fatigue relief was significantly reduced in the hUC-MSCs group (25% [2/8]) compared to the control group (76.5% [13/17]) (P = 0.028). The level of KL-6 was significantly lower in the hUC-MSCs group (2585.5 ± 186.5 U/ml) than in the control group (3120.7 ± 158.3 U/ml) (P < 0.001). Compared with the control group, the hUC-MSCs group had a lower level of MDA (9.27 ± 0.54 vs. 9.91 ± 0.72 nmol/ml, P = 0.036). No obvious adverse effects were observed in the hUC-MSCs treatment group at 1 year after discharge. CONCLUSIONS: Intravenous transplantation of hUC-MSCs was a safe approach in the long term in the treatment of patients with severe COVID-19. In addition, hUC-MSCs had a positive effect on postinfection sequelae in COVID-19 survivors. TRIAL REGISTRATION: Chinese Clinical Trial Registration; ChiCTR2000031494; Registered 02 April 2020-Retrospectively registered, http://www.medresman.org.


Asunto(s)
COVID-19 , Trasplante de Células Madre Mesenquimatosas , Células Madre Mesenquimatosas , COVID-19/terapia , Fatiga , Estudios de Seguimiento , Humanos , Cordón Umbilical
15.
Dig Endosc ; 2022 Jul 23.
Artículo en Inglés | MEDLINE | ID: mdl-35869797

RESUMEN

OBJECTIVES: To evaluate the safety and efficacy of a new endoscopic duodenal-jejunal bypass sleeve (DJBS) in obese patients with non-alcoholic fatty liver disease (NAFLD), while in situ for 3 months, and at 6 months post-explantation. METHODS: Patients with obesity and NAFLD were enrolled in this single-center, prospective study, wherein TONGEE® DJBS was implanted for 3 months. Primary outcomes were weight loss and changes in hepatic steatosis. Secondary outcomes included changes in liver enzymes, glycemic control, and lipid profile and device safety. RESULTS: Twenty-six patients (age: 35.2 ± 7.2 years; 61.5% women) underwent DJBS implantation. At 3 months, body weight change from baseline was -8.0 ± 3.6 kg (P < 0.001), corresponding to 8.9 ± 4.0% of total body weight. Hepatic steatosis significantly improved based on controlled attenuation parameter, hepatic steatosis index, and fatty liver index (P < 0.001). Liver enzymes, insulin resistance, and metabolic parameters were also improved. At 6 months post-explantation, weight loss and improvements in hepatic steatosis and liver enzyme levels remained statistically significant. Only one patient had a serious adverse event, namely, upper gastrointestinal hemorrhage. CONCLUSIONS: Three-month TONGEE® DJBS implantation resulted in significant weight loss and improvement in hepatic steatosis, liver enzymes, insulin resistance, and metabolic parameters in obese patients with NAFLD. Randomized controlled trials are required to further elucidate these initial findings.

16.
Biosens Bioelectron ; 214: 114500, 2022 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-35797936

RESUMEN

Aflatoxin B1 (AFB1) is a highly toxic fungal contaminant widely found in agricultural products. It causes serious harm to human health and the environment. Thus, a fast and sensitive detection approach is urgently needed to prevent AFB1-contaminated products from entering the market effectively. A photoelectrochemical (PEC) immunosensor was developed based on tungsten trioxide/cadmium sulfide core/shell coated with a composite layer consisting of polydopamine and loaded gold nanoparticles (WO3/CdS@PDA/Au) for AFB1 detection. CdS formed a heterojunction with WO3, which improved the photoelectric performance. The coated PDA reducing CdS toxicity was demonstrated by biological experiment of Bacillus subtilis. PDA and Au NPs promoted electron transfer between the semiconductors, being beneficial promoting the photoelectron transfer. Additionally, the antibodies were immobilized on WO3/CdS@PDA/Au via the reactive quinones on the surface of the PDA and electrostatic adsorption from Au NPs. The WO3/CdS@PDA/Au composite as a Z-scheme heterojunction possessed high performance of photocurrent response, and the photoproduced electron/hole transfer path was speculated by electrons spin-resonance spectroscopy technique. Under the optimum experimental conditions, the PEC immunosensor showed a wide linear detection range from 0.05 to 100 ng mL-1 for AFB1, indicating that the immunosensor has a bright application prospect.


Asunto(s)
Técnicas Biosensibles , Nanopartículas del Metal , Aflatoxina B1 , Técnicas Biosensibles/métodos , Técnicas Electroquímicas/métodos , Oro/química , Humanos , Inmunoensayo/métodos , Límite de Detección , Nanopartículas del Metal/química
17.
J Biomed Nanotechnol ; 18(4): 1075-1083, 2022 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-35854459

RESUMEN

Lung cancer is the most common cancer throughout the world. Currently, most lung cancer therapies are still limited by serious side effects caused. This paper reports a biocompatible drug delivery system that utilizes milk-derived exosomes to deliver paclitaxel to treat lung adenocarcinoma. First, milk-derived exosomes were modified with integrin αVß3, αVß5-binding peptide iRGD so that they could successfully target lung adenocarcinoma cells. Then, iRGD modified exosomes were loaded with paclitaxel (PAC) via electroporation and used for tumor therapy. These modified exosomes proved effective in killing lung adenocarcinoma cells, and the exosome-based nanoplatform showed no obvious toxicity to normal cells. Further more, the exosome-based nanoplatform could effectively penetrate the interior of the 3D tumor sphere, reaching more tumor cells and demonstrating that it is a promising tool for lung adenocarcinoma therapy.


Asunto(s)
Adenocarcinoma del Pulmón , Exosomas , Neoplasias Pulmonares , Adenocarcinoma del Pulmón/tratamiento farmacológico , Animales , Línea Celular Tumoral , Humanos , Neoplasias Pulmonares/tratamiento farmacológico , Leche , Paclitaxel
18.
ACS Appl Bio Mater ; 5(7): 3509-3518, 2022 Jul 18.
Artículo en Inglés | MEDLINE | ID: mdl-35793521

RESUMEN

Medical protective materials have broadly drawn attention due to their ability to stop the spread of infectious diseases and protect the safety of medical staff. However, creating medical protective materials that combine excellent liquid shielding performance and outstanding mechanical properties with high breathability is still a challenging task. Herein, a polyester/polyamide 6 (PET/PA6) bicomponent microfilament fabric with tunable porosity for comfortable medical protective clothing was prepared via dip-coating technology and an easy and effective thermal-belt bonding process. The dip coating of the C6-based fluorocarbon polymer endowed the samples with excellent hydrophobicity (alcohol contact angles, 130-128°); meanwhile, by adjusting the temperature and pressure of the thermal-belt bonding process, the porosity of the samples was adapted in the range of 64.19-88.64%. Furthermore, benefitting tunable porosity and surface hydrophobicity, the samples also demonstrated an excellent softness score (24.3-34.5), agreeable air permeability (46.3-27.8 mm/s), and high hydrostatic pressure (1176-4130 Pa). Significantly, the created textiles successfully filter aerosol from the air and display highly tensile strength. These excellent comprehensive performances indicate that the prepared PET/PA6 bicomponent microfilament fabrics would be an attractive choice for medical protective apparel.


Asunto(s)
Poliésteres , Textiles , Citoesqueleto de Actina , Caprolactama/análogos & derivados , Humanos , Polímeros , Porosidad , Tomografía de Emisión de Positrones , Ropa de Protección
19.
Nat Commun ; 13(1): 3850, 2022 Jul 04.
Artículo en Inglés | MEDLINE | ID: mdl-35787630

RESUMEN

Heart failure with preserved ejection fraction (HFpEF) exhibits a sex bias, being more common in women than men, and we hypothesize that mitochondrial sex differences might underlie this bias. As part of genetic studies of heart failure in mice, we observe that heart mitochondrial DNA levels and function tend to be reduced in females as compared to males. We also observe that expression of genes encoding mitochondrial proteins are higher in males than females in human cohorts. We test our hypothesis in a panel of genetically diverse inbred strains of mice, termed the Hybrid Mouse Diversity Panel (HMDP). Indeed, we find that mitochondrial gene expression is highly correlated with diastolic function, a key trait in HFpEF. Consistent with this, studies of a "two-hit" mouse model of HFpEF confirm that mitochondrial function differs between sexes and is strongly associated with a number of HFpEF traits. By integrating data from human heart failure and the mouse HMDP cohort, we identify the mitochondrial gene Acsl6 as a genetic determinant of diastolic function. We validate its role in HFpEF using adenoviral over-expression in the heart. We conclude that sex differences in mitochondrial function underlie, in part, the sex bias in diastolic function.


Asunto(s)
Insuficiencia Cardíaca , Animales , Coenzima A Ligasas , Diástole/genética , Femenino , Insuficiencia Cardíaca/metabolismo , Humanos , Masculino , Ratones , Mitocondrias Cardíacas/genética , Mitocondrias Cardíacas/metabolismo , Caracteres Sexuales , Volumen Sistólico/genética
20.
Food Chem ; 397: 133746, 2022 Jul 19.
Artículo en Inglés | MEDLINE | ID: mdl-35882166

RESUMEN

Intensive fattening usually results in the changes of meat quality. Tenderness is a central attribute for mutton sensory qualities and consumers' choice. Here, we reported that intensive fattening mutton was more tender than that of traditionally raised sheep. By proteomic approach, we found 49 differentially expressed proteins in longissimus dorsi muscle. After bioinformatics analysis, 5 cytoskeletal proteins, 3 protein binding proteins and 7 metabolic enzymes were identified as potential biomarkers for mutton tenderness. Finally, we verified the expression of these abundant proteins by parallel reaction monitoring (PRM). Collectively, our results reveal that the mutton of sheep raised by intensive fattening is more tender than that of traditionally raised sheep. Myosin-2, myosin-13, vimentin, carbonic anhydrase, carbonic anhydrase-2, Glutathione S-transferase and Microtubule-associated protein 4 isoform X1 can be candidate biomarkers for mutton tenderness. Our data also indicate a central role of cytoskeletal proteins and metabolic enzymes in determining mutton tenderness.

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