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1.
Dermatol Ther ; : e13564, 2020 May 13.
Artículo en Inglés | MEDLINE | ID: mdl-32400925

RESUMEN

BACKGROUND: The treatment of keloids and hypertrophic scars remains a challenge. Although triamcinolone acetonide (TAC) is one of the most common and effective treatments for keloids and hypertrophic scars, TAC is not effective in some patients, and some may even experience adverse outcomes. Verapamil might be considered a safe alternative to TAC. The aim of this study was to compare the efficacy and safety of verapamil and TAC for the treatment of keloids and hypertrophic scars. METHODS: Three databases (Medline, EMBASE, and CENTRAL database) were electronically searched from 1997 to December 2019. Article selection was limited to randomized controlled trials (RCTs) and controlled clinical trials (CCTs). Two authors independently assessed the selection of studies, risk of bias, and extracted the data. Mean differences (MDs) were computed for continuous variables, risk ratios (RRs) were computed for dichotomous variables, and 95% confidence intervals (CIs) were calculated for both assessments. RESULTS: Five RCTs were included, comprising a total of 215 patients (273 scars). Vancouver Scar Scale (VSS) parameters (height, vascularity, pliability, and pigmentation) were reported as the outcome measures and provided detailed values in four studies. No significant differences were observed between verapamil and TAC in the reduction of height (MD 0.57, 95%CI -0.94 to 2.08, P = 0.46), vascularity (MD 0.30, 95%CI -0.42 to 1.02, P = 0.41), pliability (MD 0.67, 95%CI -1.12 to 2.47, P = 0.46), and degree of pigmentation (MD 0.14, 95%CI -0.41 to 0.69, P = 0.61). Adverse outcomes were reported in four studies. The results showed that the incidence of telangiectasia and skin atrophy when verapamil was used were significantly lower than for TAC. CONCLUSION: Concerning the treatment of keloids and hypertrophic scars, even though verapamil was safer than TAC, TAC worked faster than verapamil. Furthermore, we did not find any clear evidence that verapamil was more or less effective than TAC. Considering the high degree of safety of verapamil, we suggest that verapamil might be used as an alternative treatment when TAC results in adverse outcomes. This article is protected by copyright. All rights reserved.

2.
Sci Total Environ ; 713: 136443, 2020 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-31954251

RESUMEN

The aerosol extinction coefficient was an important factor for air quality. To estimate the aerosol extinction levels, widely used pure statistical models are generally not based on aerosol vertical structures. In this study, we estimated large-scale aerosol extinction coefficients by developing a new layer-resolved model with explicit inference for aerosol vertical distribution. The CALIOP aerosol profile, MODIS AOD and reanalysis boundary layer height data are used. The layer-resolved model was formulated by developing an explicit, steady and straightforward relationship between aerosol within boundary layer and corresponding AOD values. The estimated surface extinction coefficient from this model was compared against the values derived from station visibility observations in China in 2016. The results revealed that our model had outperformed the traditional one-layer model and the simplified two-layer model. Specifically, the numbers of ground stations with an NME value < 0.4 are enhanced by a percentage > 100%, with the NME values significantly decreased from 46%, 48% to 36% and RMSE values from 0.27, 0.25 to 0.21 km-1. Our model is easy for operational implementation thanks to its clear structure and input, and also informative to understand aerosol vertical distributions. Furthermore, this work will also be beneficial to air quality modeling studies to improve accuracy estimating ground-level PM2.5 concentrations.

3.
J Pain Res ; 12: 2817-2823, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31632131

RESUMEN

Objective: Microvascular decompression (MVD) combined with partial sensory rhizotomy (PSR) with the retrosigmoid approach has become the most effective surgical treatment for trigeminal neuralgia (TN). There is variability in the pain relief processes observed in postoperative patients. The purpose of this study was to investigate delayed relief (DR) and its predictors after MVD and/or PSR for the treatment of TN and study the long-term effects associated with DR. Methods: Patients with primary TN who underwent MVD and/or PSR by the same surgeon at the China-Japan Friendship Hospital from March 2009 to December 2017 were included in the study, and all patients were followed for at least 1 year after the operation. DR was defined as follows: no changes in the Barrow Neurological Institute (BNI) score on the third day after surgery and a BNI score of I-II in the absence of any medication after a period of pain. Preoperative, intraoperative and postoperative differences were compared between the DR and non-DR groups, and the relationships between the various factors and DR was analyzed. Results: A total of 105 patients, including 20 patients with DR (19%), 78 patients with non-DR (74%), and 7 patients without relief, were included in this study. The follow-up period ranged from 13 months to 118 months (average, 5.39 years/65 months). The duration of postoperative pain in the DR group was 3-365 days, with an average of 108 days. Statistical analysis found that no factor predicted the occurrence of DR, and the occurrence of postoperative DR did not affect the long-term effects observed in patients. Conclusion: DR did not affect the long-term effects after MVD and/or PSR. Therefore, it is recommended that patients should be monitored for approximately 3 months after MVD and/or PSR and then evaluated for surgical effects. No reoperations should be performed immediately.

4.
Environ Sci Technol ; 53(13): 7306-7315, 2019 07 02.
Artículo en Inglés | MEDLINE | ID: mdl-31244060

RESUMEN

Atmospheric chemical transport models (CTMs) have been widely used to simulate spatiotemporally resolved PM2.5 concentrations. However, CTM results are usually prone to bias and errors. In this study, we improved the accuracy of PM2.5 predictions by developing an ensemble deep learning framework to fuse model simulations with ground-level observations. The framework encompasses four machine-learning models, i.e., general linear model, fully connected neural network, random forest, and gradient boosting machine, and combines them by stacking approach. This framework is applied to PM2.5 concentrations simulated by the Community Multiscale Air Quality (CMAQ) model for China from 2014 to 2017, which has complete spatial coverage over the entirety of China at a 12-km resolution, with no sampling biases. The fused PM2.5 concentration fields were evaluated by comparing with an independent network of observations. The R2 values increased from 0.39 to 0.64, and the RMSE values decreased from 33.7 µg/m3 to 24.8 µg/m3. According to the fused data, the percentage of Chinese population residing under the level II National Ambient Air Quality Standards of 35 µg/m3 for PM2.5 has increased from 46.5% in 2014 to 61.7% in 2017. The method is readily adapted to utilize near-real-time observations for operational analyses and forecasting of pollutant concentrations and can be extended to provide source apportionment forecasts as well.


Asunto(s)
Contaminantes Atmosféricos , Contaminación del Aire , China , Aprendizaje Profundo , Monitoreo del Ambiente , Material Particulado
5.
Psychol Trauma ; 11(7): 760-766, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-30907612

RESUMEN

OBJECTIVE: The aim of this study was to examine the comorbidity and longitudinal cross-lagged relationships between posttraumatic stress disorder (PTSD) and depression among Chinese adolescents after their experience of the Yancheng tornado. METHOD: A total of 154 adolescents from 2 local middle schools in Yancheng city participated in this longitudinal study. Participants completed the Child PTSD Symptom Scale and the Center Epidemiological Studies of Depression at 6 months (T1), 9 months (T2), and 12 months (T3) after the tornado, respectively. RESULTS: Results include the following: (a) the prevalence of PTSD was 55.84% at T1, 50.00% at T2, and 47.40% at T3; the prevalence of depression was 56.49% at T1, 65.58% at T2, and 66.01% at T3; (b) the comorbidity between PTSD and depression was high, 46.75% at T1, 42.86% at T2, 43.51% at T3; (c) PTSD at T1 significantly predicted depression at T2 (ß = 0.42, p < .001), and PTSD at T2 significantly predicted depression at T3 (ß = 0.64, p < .001); however, depression cannot significantly predict PTSD at different cross-time points. CONCLUSIONS: The results suggest that there is comorbidity between PTSD and depression and that PTSD positively influence the development of depression in adolescents during the early period of the tornado, whereas depression did not predict PTSD. The implications of the results for the psychological services provided to children and youth are discussed. (PsycINFO Database Record (c) 2019 APA, all rights reserved).


Asunto(s)
Depresión/epidemiología , Trastorno Depresivo/epidemiología , Trastornos por Estrés Postraumático/epidemiología , Sobrevivientes/estadística & datos numéricos , Tornados/estadística & datos numéricos , Adolescente , China/epidemiología , Comorbilidad , Femenino , Humanos , Estudios Longitudinales , Masculino , Prevalencia
6.
Front Psychol ; 9: 27, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29441031

RESUMEN

Children's block building performances are used as indicators of other abilities in multiple domains. In the current study, we examined individual differences, types of model and social settings as influences on children's block building performance. Chinese preschoolers (N = 180) participated in a block building activity in a natural setting, and performance was assessed with multiple measures in order to identify a range of specific skills. Using scores generated across these measures, three dependent variables were analyzed: block building skills, structural balance and structural features. An overall MANOVA showed that there were significant main effects of gender and grade level across most measures. Types of model showed no significant effect in children's block building. There was a significant main effect of social settings on structural features, with the best performance in the 5-member group, followed by individual and then the 10-member block building. These findings suggest that boys performed better than girls in block building activity. Block building performance increased significantly from 1st to 2nd year of preschool, but not from second to third. The preschoolers created more representational constructions when presented with a model made of wooden rather than with a picture. There was partial evidence that children performed better when working with peers in a small group than when working alone or working in a large group. It is suggested that future study should examine other modalities rather than the visual one, diversify the samples and adopt a longitudinal investigation.

7.
Org Biomol Chem ; 15(30): 6367-6374, 2017 Aug 02.
Artículo en Inglés | MEDLINE | ID: mdl-28717802

RESUMEN

This paper reports a computational study elucidating the reaction mechanism for ynamide-mediated amide bond formation from carboxylic acids and amines. The mechanisms have been studied in detail for ynamide hydrocarboxylation and the subsequent aminolysis of the resulting adduct by an amine. Ynamide hydrocarboxylation is kinetically favorable and thermodynamically irreversible, resulting in the formation of a key low-lying intermediate CP1 featuring geminal vinylic acyloxy and sulfonamide groups. The aminolysis of CP1 by the amine is proposed to be catalyzed by the carboxylic acid itself that imparts favourable bifunctional effects. In the proposed key transition state TSaminolysis-acid-iso2, the amine undergoes direct nucleophilic substitution at the acyl of CP1 to replace the enolate group in a concerted way, which is promoted by secondary hydrogen bonding of carboxylic acid with both the amine and CP1. These secondary interactions are suggested to increase the nucleophilicity of the amine and to activate the Cacyl-O bond to be cleaved, thereby stabilizing the aminolysis transition state. The concerted aminolysis mechanism is competitive with the classic stepwise nucleophilic acyl substitution mechanism that features sequential amine addition to acyl/intramolecular proton transfer/C-O bond cleavage and a key tetrahedral intermediate. Based on the mechanistic model, the carboxylic acid substrate effect and studies of more acidic CF3SO3H as the catalyst are in good agreement with the experimental observations, lending further support for the mechanistic model. The bifunctional catalytic effect of the carboxylic acid substrate may widely play a role in related amide bond-forming reactions and peptide formation chemistry.

8.
Am J Transl Res ; 9(12): 5696-5707, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-29312522

RESUMEN

BACKGROUND: Bronchopulmonary dysplasia (BPD) in premature infants is a predominantly secondary occurrence to intrauterine inflammation/infection and postpartum mechanical ventilation; The purpose of this study is to explore the biological roles of lincRNA in the pathogenesis of BPD. METHODS: Newborn rats were randomly assigned to hyperoxia (85% O2) or the control group: the normoxia group (21% O2). Lung tissues were collected on days 1-14. The BPD animal model was validated using HE staining, Masson staining, and real-time RT-PCR. Deep Illumina sequencing was used to reveal the differential expression of long non-coding RNAs in hyperoxia bronchopulmonary dysplasia rat models. KEGG and GO functions were predicted. Nine possible BPD-related target lincRNAs were verified by RTq-PCR. RESULTS: The histopathologic changes in lung tissues manifested as hyperaemia, edema, hemorrhage, and inflammation cell infiltration after continuous exposure to hyperoxia for 3 days, and became aggravated after 7 days of hyperoxic exposure. The above lung tissue inflammatory manifestations were alleviated and taken over by pulmonary interstitia hyperplasia and fibrocyte proliferation after 14 days of hyperoxic exposure. The expressions of lincRNA differed between the hyperoxia bronchopulmonary dysplasia model group and the normoxia group. 1175 different lincRNAs were detected in the hyperoxia group and the normoxia group, of which 544 were up-regulated and 631 were down-regulated. 673 moleculars related to GO functions were enriched, including cell location and biological process. Pathway enrichment analysis showed that lincRNA was involved in 257 KEGG pathways. 9 lincRNA were validated in the sample, and the difference was statistically significant. CONCLUSION: LincRNAs were identified differently between the BPD model and the normoxia group. Many target genes were involved in the developmental process, including cell component biogenesis, biological regulation, transcription regulator, and translation regulator. The BPD might be caused by the activation of the pathways of the EMC-receptor interaction, cytokine-cytokine receptor interaction, cell cycle, and cell adhesion molecules. The present study provides new insight into the pathogenesis mechanism of BPD.

9.
Phys Chem Chem Phys ; 18(48): 32664-32667, 2016 Dec 07.
Artículo en Inglés | MEDLINE | ID: mdl-27892558

RESUMEN

Reductive elimination from Pd(ii) aryl trifluoromethyl complexes is a challenging and elusive step which is accompanied by a number of kinetically more favorable side reactions giving rising to a complex mixture. We report herein the synthesis and isolation of several arylPd(ii) trifluoromethyl complexes (2a-c) and study their electronic structures, photophysical properties and reductive elimination reactivities. A remarkable concentration effect on chemoselectivity is observed for thermal decomposition of (Xantphos)Pd(ii)(Ar)(CF3) (2c) that favors the formation of Ar-CF3 at lower concentrations, but gives increasingly more Ar-Ar homocoupling product to a dominant extent as the concentration of 2c increases. This is solid evidence for the involvement of an intermolecular Ar/CF3 ligand exchange/Ar-Ar reductive elimination mechanism that has been proposed based on DFT computational studies. The interplay between theory and experiment provides valuable insights into the mechanism and kinetics of the key elementary reaction of reductive elimination at Pd(ii), and may thus prompt the design of more efficient Pd-mediated nucleophilic trifluoromethylation reactions.

10.
Org Biomol Chem ; 14(38): 8966-8970, 2016 Sep 26.
Artículo en Inglés | MEDLINE | ID: mdl-27714212

RESUMEN

A Cu(i)-catalyzed retro-aldol reaction of ß-hydroxyketones with ortho-aminobenzaldehydes and nicotinaldehydes is reported that produces a range of quinolines and naphthyridines with high efficiency and selectivity. This reaction uses ß-hydroxyketones as a regiospecific ketone-protected enolate source via copper-catalyzed retro-aldol Cα-Cß bond cleavage. The in situ generated copper enolate undergoes kinetically favorable cyclization with ortho-amino aryl aldehydes to produce quinolines and naphthyridines in a chemo- and regioselective manner. The mild and weakly basic reaction conditions also suppress possible side reactions of benzaldehydes under strongly basic conditions, resulting in improved reaction yields.

11.
Zhongguo Dang Dai Er Ke Za Zhi ; 18(9): 851-856, 2016 Sep.
Artículo en Chino | MEDLINE | ID: mdl-27655543

RESUMEN

OBJECTIVE: To investigate the nutritional status of school-age children in rural area in Hunan, China from 2012 to 2015 and to evaluate the effectiveness of the "Nutrition Improvement Program for Compulsory Education Students in Rural Area" (hereinafter referred to as "Nutrition Improvement Program"). METHODS: The nutritional status of school-age children aged 6-14 years was evaluated after the implementation of the "Nutrition Improvement Program" and the changing trend of the children's nutritional status was analyzed. The statistical analysis was performed on the monitoring data of the school-age children aged 6-14 years in rural area in Hunan, China from 2012 to 2015, which came from "The Nutrition and Health Status Monitoring and Evaluation System of Nutrition Improvement Program for Compulsory Education Students in Rural Area". RESULTS: In 2015, female students aged 6-7 years in rural area in Hunan, China had a significantly greater body length than the rural average in China (P<0.05). However, the other age groups had significantly smaller body length and weight than the rural averages in China (P<0.05). After the implementation of "Nutrition Improvement Program", the prevalence rate of growth retardation decreased (P<0.05), but the prevalence rate of emaciation increased (P<0.05). At the same time, the prevalence rate of overweight/obesity increased (P<0.05) and the prevalence rate of anemia decreased (P<0.05). CONCLUSIONS: The implementation of "Nutrition Improvement Program" has achieved some success, but the nutritional status of school-age children has not improved significantly. Overweight/obesity and malnutrition are still present. Therefore, to promote the nutritional status of school-age children it is recommended to improve the measures for the "Nutrition Improvement Program".


Asunto(s)
Fenómenos Fisiológicos Nutricionales Infantiles , Estado Nutricional , Adolescente , Niño , China , Femenino , Humanos , Masculino , Población Rural
12.
Org Biomol Chem ; 14(30): 7282-94, 2016 Jul 26.
Artículo en Inglés | MEDLINE | ID: mdl-27397647

RESUMEN

A copper-catalyzed transfer aldol type reaction of ß-hydroxy ketones or nitriles with aldehydes is reported, which enables chemo- and stereoselective access to (E)-α,ß-unsaturated ketones and (E)-acrylonitriles. A key step of the in situ copper(i)-promoted retro-aldol reaction of ß-hydroxy ketones or nitriles is proposed to generate a reactive Cu(i) enolate or cyanomethyl intermediate, which undergoes ensuing aldol condensation with aldehydes to deliver the products. This reaction uses 1.2 mol% Cu(IPr)Cl (IPr denotes 1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene) as the catalyst in the presence of 6.0 mol% NaOtBu cocatalyst at room temperature or 70 °C. A range of aryl and heteroaryl aldehydes as well as acrylaldehydes are compatible with many useful functional groups being tolerated. Under the mild and weakly basic conditions, competitive Cannizzaro-type reaction of benzaldehydes and side reactions of base-sensitive functional groups can be effectively suppressed, which show synthetic advantages of this reaction compared to classic aldol reactions. The synthetic potential of this reaction is further demonstrated by the one-step synthesis of biologically active quinolines and 1,8-naphthyridine in excellent yields (up to 91%). Finally, a full catalytic cycle for this reaction has been constructed using DFT computational studies in the context of a retro-aldol/aldol two-stage mechanism. A rather flat reaction energy profile is found indicating that both stages are kinetically facile, which is consistent with the mild reaction conditions.

13.
Nucleic Acids Res ; 44(8): 3629-42, 2016 05 05.
Artículo en Inglés | MEDLINE | ID: mdl-26704979

RESUMEN

Dicer participates in heterochromatin formation in fission yeast and plants. However, whether it has a similar role in mammals remains controversial. Here we showed that the human Dicer protein interacts with SIRT7, an NAD(+)-dependent H3K18Ac (acetylated lysine 18 of histone H3) deacetylase, and holds a proportion of SIRT7 in the cytoplasm. Dicer knockdown led to an increase of chromatin-associated SIRT7 and simultaneously a decrease of cytoplasmic SIRT7, while its overexpression induced SIRT7 reduction in the chromatin-associated fraction and increment in the cytoplasm. Furthermore, DNA damaging agents promoted Dicer expression, leading to decreased level of chromatin-associated SIRT7 and increased level of H3K18Ac, which can be alleviated by Dicer knockdown. Taken together with that H3K18Ac was exclusively associated with the chromatin, our findings suggest that Dicer induction by DNA damaging treatments prevents H3K18Ac deacetylation, probably by trapping more SIRT7 in the cytoplasm.


Asunto(s)
ARN Helicasas DEAD-box/metabolismo , Daño del ADN , Histonas/metabolismo , Ribonucleasa III/metabolismo , Sirtuinas/metabolismo , Línea Celular , Cromatina/metabolismo , Cisplatino/toxicidad , ARN Helicasas DEAD-box/antagonistas & inhibidores , Doxorrubicina/toxicidad , Células HEK293 , Humanos , Radiación Ionizante , Ribonucleasa III/antagonistas & inhibidores
14.
Sci Rep ; 5: 9457, 2015 Mar 25.
Artículo en Inglés | MEDLINE | ID: mdl-25807460

RESUMEN

Grain size is an important trait influencing both the yield and quality of rice and its major determinant is glume size. However, how glume size is regulated remains largely unknown. Here, we report the characterization of OsKinesin-13A, which regulates cell elongation and glume length in rice. The mutant of OsKinesin-13A, sar1, displayed length reduction in grains and other organs including internodes, leaves and roots. The grain phenotype in sar1 was directly caused by reduction in glume length, which in turn restricted caryopsis size. Histological results revealed that length decrease in sar1 organs resulted from abnormalities in cell elongation. The orientation of cellulose microfibrils was defective in sar1. Consistently, sar1 showed reduced transverse orientation of cortical microtubules. Further observations demonstrated that microtubule turnover was decreased in sar1. OsKinesin-13A was shown to be an active microtubule depolymerase and mainly distributed on vesicles derived from the Golgi apparatus and destined for the cell surface. Thus, our results suggest that OsKinesin-13A utilizes its microtubule depolymerization activity to promote microtubule turnover, which may not only influence transverse orientation of cortical microtubules but also facilitate vesicle transport from the Golgi apparatus to the cell surface, and thus affects cellulose microfibril orientation and cell elongation.


Asunto(s)
Cinesina/genética , Microtúbulos/genética , Oryza/genética , Proteínas de Plantas/genética , Membrana Celular/metabolismo , Pared Celular/enzimología , Celulosa/genética , Celulosa/metabolismo , Aparato de Golgi/genética , Cinesina/metabolismo , Microtúbulos/metabolismo , Oryza/crecimiento & desarrollo , Hojas de la Planta/enzimología , Raíces de Plantas/enzimología
15.
Carcinogenesis ; 35(9): 2127-33, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-24913918

RESUMEN

Hepatitis B virus surface antigen (HBsAg) is an important risk factor for hepatocellular carcinoma (HCC) and is downregulated during hepatocarcinogenesis. MicroRNAs (miRNAs) are frequently deregulated in HCC tissues. However, whether the deregulation of certain miRNAs in HCC has an impact on HBsAg expression remains unclear. We found here that microRNA-581 (miR-581), which is deregulated during hepatocarcinogenesis, promoted HBsAg expression. Additionally, miR-581 targeted Dicer and endoplasmic reticulum degradation-enhancing alpha-mannosidase-like protein 1 (EDEM1) and repressed their expression. Although Dicer cannot process HBV transcripts, Dicer knockdown led to increased HBsAg secretion, most likely due to a reduction in the levels of Dicer-processed 7SL RNA fragments. Moreover, Dicer-processed 7SL RNA fragments partially inhibited the ability of miR-581 to stimulate HBsAg expression. Furthermore, we found that forced EDEM1 expression inhibited miR-581-mediated induction of HBsAg. Finally, transfection of miR-581 into HepG2.2.15 cells promoted cell proliferation and led to upregulation of genes involved in development, cell proliferation and protein secretion. Altogether, we conclude that miR-581 promotes HBsAg expression by targeting Dicer and EDEM1. Our findings suggest that downregulation of miR-581 during hepatocarcinogenesis may lead to a reduction in HBsAg expression and impede HCC development.


Asunto(s)
ARN Helicasas DEAD-box/genética , Antígenos de Superficie de la Hepatitis B/metabolismo , Proteínas de la Membrana/genética , MicroARNs/genética , Ribonucleasa III/genética , Regiones no Traducidas 3' , Sitios de Unión , ARN Helicasas DEAD-box/metabolismo , Regulación Neoplásica de la Expresión Génica , Regulación Viral de la Expresión Génica , Células Hep G2 , Humanos , Proteínas de la Membrana/metabolismo , Interferencia de ARN , Ribonucleasa III/metabolismo
16.
Ophthalmologica ; 232(2): 110-7, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24516061

RESUMEN

PURPOSE: To evaluate the relationship between lumican polymorphisms and high myopia in Chinese populations. METHODS: An electronic search was conducted in Pubmed, Embase, Cochrane Library and the China Biological Medicine Database for articles published prior to September 30, 2012. A meta-analysis was performed to assess heterogeneity, combine results and determine publication bias. RESULTS: This meta-analysis, including 1,545 subjects from 5 studies, indicated that Chinese lumican rs3759223 C allele carriers had a decreased risk of high myopia in comparison to T allele carriers (odds ratio: 0.531; 95% confidence interval, CI: 0.304-0.925; p = 0.025). There was some heterogeneity between studies. A metaregression showed that the mean axial length of controls weakens the effect of rs3759223 on high myopia (slope: -0.914; 95% CI: -1.490 to 0.337; p = 0.002). Sensitivity analysis confirmed the reliability and stability of this meta-analysis. CONCLUSION: Chinese lumican rs3759223 C allele carriers may be at reduced risk of high myopia.


Asunto(s)
Grupo de Ascendencia Continental Asiática/genética , Proteoglicanos Tipo Condroitín Sulfato/genética , Sulfato de Queratano/genética , Miopía Degenerativa/genética , Polimorfismo de Nucleótido Simple , Regiones Promotoras Genéticas/genética , Adulto , Estudios de Casos y Controles , China/epidemiología , Femenino , Genotipo , Humanos , Lumican , Masculino , Miopía Degenerativa/etnología , Adulto Joven
17.
J Gastroenterol Hepatol ; 29(6): 1149-58, 2014 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-24476011

RESUMEN

BACKGROUND AND AIM: Controlled attenuation parameter (CAP) is a novel ultrasound-based elastography method for detection of steatosis severity. This meta-analysis aimed to assess the performance of CAP. METHODS: PubMed, the Cochrane Library, and the Web of Knowledge were searched to find studies, published in English, relating to accuracy evaluations of CAP for detecting stage 1 (S1), stage 2 (S2), or stage 3 (S3) hepatic steatosis which was diagnosed by liver biopsy. Sensitivities, specificities, and hierarchical summary receiver operating characteristic (HSROC) curves were used to examine CAP performance. The clinical utility of CAP was also evaluated. RESULTS: Nine studies, with 11 cohorts were analyzed. The summary sensitivities and specificities values were 0.78 (95% confidence interval [CI], 0.69-0.84) and 0.79 (95% CI, 0.68-0.86) for ≥ S1, 0.85 (95% CI, 0.74-0.92) and 0.79 (95% CI, 0.71-0.85) for ≥ S2, and 0.83 (95% CI, 0.76-0.89) and 0.79 (95% CI, 0.68-0.87) for ≥ S3. The HSROCs were 0.85 (95% CI, 0.81-88) for ≥ S1, 0.88 (95% CI, 0.85-0.91) for ≥ S2, and 0.87 (95% CI, 0.84-0.90) for ≥ S3. Following a "positive" measurement (over the threshold value) for ≥ S1, ≥ S2, and ≥ S3, the corresponding post-test probabilities for the presence of steatosis (pretest probability was 50%) were 78%, 80% and 80%, respectively; if the values were below these thresholds ("negative" results), the post-test probabilities were 22%, 16%, and 17%, respectively. CONCLUSIONS: CAP has good sensitivity and specificity for detecting hepatic steatosis; however, based on a meta-analysis, CAP was limited in their accuracy of steatosis, which precluded widespread use in clinical practice.


Asunto(s)
Bases de Datos Bibliográficas , Diagnóstico por Imagen de Elasticidad/métodos , Hígado Graso/diagnóstico , Hepatopatías/diagnóstico , Ultrasonografía/métodos , Enfermedad Crónica , Estudios de Cohortes , Humanos , Curva ROC , Sensibilidad y Especificidad , Índice de Severidad de la Enfermedad
18.
Carcinogenesis ; 35(1): 155-63, 2014 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-23917076

RESUMEN

Hepatitis B surface antigen (HBsAg) seropositivity is an important risk factor for hepatocellular carcinoma (HCC), and HBsAg-transgenic mice have been reported to spontaneously develop HCC. The major histocompatibility complex class I-related molecules A and B (MICA and MICB) are NKG2D ligands that play important roles in tumor immune surveillance. In the present study, we found that HBsAg overexpression in HepG2 cells led to upregulation of 133 and downregulation of 9 microRNAs (miRNAs). Interestingly, several HBsAg-induced miRNAs repressed the expression of MICA and MICB via targeting their 3'-untranslated regions. In addition, the expression of MICA and MICB was significantly reduced upon HBsAg overexpression, which was partially restored by inhibiting the activities of HBsAg-induced miRNAs. Moreover, HBsAg-overexpressing HCC cells exhibited reduced sensitivity to natural killer cell-mediated cytolysis. Taken together, our data suggest that HBsAg supresses the expression of MICA and MICB via induction of cellular miRNAs, thereby preventing NKG2D-mediated elimination of HCC cells.


Asunto(s)
Carcinoma Hepatocelular/virología , Antígenos de Superficie de la Hepatitis B/metabolismo , Antígenos de Histocompatibilidad Clase I/metabolismo , Neoplasias Hepáticas/virología , MicroARNs/genética , Carcinoma Hepatocelular/genética , Regulación Neoplásica de la Expresión Génica , Células Hep G2/virología , Antígenos de Superficie de la Hepatitis B/genética , Virus de la Hepatitis B/patogenicidad , Virus de la Hepatitis B/fisiología , Antígenos de Histocompatibilidad Clase I/genética , Humanos , Células Asesinas Naturales/metabolismo , Células Asesinas Naturales/patología , Neoplasias Hepáticas/genética
19.
PLoS One ; 8(10): e76322, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24204613

RESUMEN

OBJECTIVES: To perform a meta-analysis assessing the ability of shear wave elastography (SWE) to identify malignant breast masses. METHODS: PubMed, the Cochrane Library, and the ISI Web of Knowledge were searched for studies evaluating the accuracy of SWE for identifying malignant breast masses. The diagnostic accuracy of SWE was evaluated according to sensitivity, specificity, and hierarchical summary receiver operating characteristic (HSROC) curves. An analysis was also performed according to the SWE mode used: supersonic shear imaging (SSI) and the acoustic radiation force impulse (ARFI) technique. The clinical utility of SWE for identifying malignant breast masses was evaluated using analysis of Fagan plot. RESULTS: A total of 9 studies, including 1888 women and 2000 breast masses, were analyzed. Summary sensitivities and specificities were 0.91 (95% confidence interval [CI], 0.88-0.94) and 0.82 (95% CI, 0.75-0.87) by SSI and 0.89 (95% CI, 0.81-0.94) and 0.91 (95% CI, 0.84-0.95) by ARFI, respectively. The HSROCs for SSI and ARFI were 0.92 (95% CI, 0.90-0.94) and 0.96 (95% CI, 0.93-0.97), respectively. SSI and ARFI were both very informative, with probabilities of 83% and 91%, respectively, for correctly differentiating between benign and malignant breast masses following a "positive" measurement (over the threshold value) and probabilities of disease as low as 10% and 11%, respectively, following a "negative" measurement (below the threshold value) when the pre-test probability was 50%. CONCLUSIONS: SWE could be used as a good identification tool for the classification of breast masses.


Asunto(s)
Neoplasias de la Mama/diagnóstico por imagen , Neoplasias de la Mama/patología , Mama/patología , Diagnóstico por Imagen de Elasticidad , Ultrasonografía Mamaria , Adulto , Anciano , Diagnóstico Diferencial , Femenino , Humanos , Persona de Mediana Edad , Curva ROC , Reproducibilidad de los Resultados , Sensibilidad y Especificidad
20.
Onkologie ; 36(10): 560-5, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24107909

RESUMEN

BACKGROUND/AIMS: To accurately evaluate the impact of the C/T polymorphism in microRNA (miRNA)-196a2 on the colorectal cancer (CRC) risk, by meta-analysis. METHODS: An electronic search for articles was conducted in PubMed, EMBASE, ISI Web of Science, and the Cochrane Library. The pooled odds ratio (OR) and its 95% confidence interval (CI) were used to assess the association through meta-analysis. RESULTS: 5 studies were used for analysis. The results showed a significant association between the miRNA-196a2 C/T polymorphism and CRC risk in the genetic models (C vs. T: OR = 1.168, 95% CI = 1.106-1.282, p = 0.001; CC vs. TT: OR = 1.368, 95% CI = 1.132-1.654, p = 0.001; TC/CC vs. TT: OR = 1.206, 95% = CI 1.035-1.405, p = 0.016; CC vs. TC/TT: OR = 1.254, 95% CI = 1.077-1.461, p = 0.004), with the exception of the TC-versus-TT model (TC vs. TT: OR = 1.130, 95% CI = 0.961-1.329, p = 0.138). In a subgroup analysis based on ethnicity, we identified a significant overrepresentation of the polymorphism in individuals of Asian ethnicity. CONCLUSION: This meta-analysis indicates a significant association between the miRNA-196a2 polymorphism and CRC risk.


Asunto(s)
Neoplasias Colorrectales/epidemiología , Neoplasias Colorrectales/genética , Marcadores Genéticos/genética , Predisposición Genética a la Enfermedad/epidemiología , Predisposición Genética a la Enfermedad/genética , MicroARNs/genética , Polimorfismo de Nucleótido Simple/genética , Femenino , Estudios de Asociación Genética , Humanos , Masculino , Prevalencia , Factores de Riesgo
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