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2.
Arq Bras Cardiol ; 116(1): 56-65, 2021 01.
Artículo en Inglés, Portugués | MEDLINE | ID: mdl-33566965

RESUMEN

BACKGROUND: Arterial hypertension (HTA) represents a major risk factor for cardiovascular morbidity and mortality. It is not yet known which specific molecular mechanisms are associated with the development of essential hypertension. OBJECTIVE: In this study, we analyzed the association between LRP1 monocyte mRNA expression, LRP1 protein expression, and carotid intima media thickness (cIMT) of patients with essential hypertension. METHODS: The LRP1 monocyte mRNA expression and protein levels and cIMT were quantified in 200 Mexican subjects, 91 normotensive (NT) and 109 hypertensive (HT). Statistical significance was defined as p < 0.05. RESULTS: HT patients group had highly significant greater cIMT as compared to NT patients (p=0.002) and this correlated with an increase in the expression of LRP1 mRNA expression (6.54 vs. 2.87) (p = 0.002) and LRP1 protein expression (17.83 vs. 6.25), respectively (p = 0.001). These differences were maintained even when we divided our study groups, taking into account only those who presented dyslipidemia in both, mRNA (p = 0.041) and proteins expression (p < 0.001). It was also found that Ang II mediated LRP1 induction on monocytes in a dose and time dependent manner with significant difference in NT vs. HT (0.195 ± 0.09 vs. 0.226 ± 0.12, p = 0.046). CONCLUSION: An increase in cIMT was found in subjects with hypertension, associated with higher mRNA and LRP1 protein expressions in monocytes, irrespective of the presence of dyslipidemias in HT patients. These results suggest that LRP1 upregulation in monocytes from Mexican hypertensive patients could be involved in the increased cIMT. (Arq Bras Cardiol. 2021; 116(1):56-65).


Asunto(s)
Grosor Intima-Media Carotídeo , Hipertensión , Humanos , Lipoproteínas LDL , Proteína 1 Relacionada con Receptor de Lipoproteína de Baja Densidad , Monocitos , Factores de Riesgo
3.
J Hum Nutr Diet ; 2021 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-33586805

RESUMEN

BACKGROUND: The fungal community of the gastrointestinal tract has recently become of interest, and knowledge of its relationship with the development of obesity is scarce. The present study aimed to evaluate the cultivable fungal fraction from the microbiota and to analyze its relationship with obesity. METHODS: Samples were taken from 99 participants with normal weight, overweight and obesity (n = 31, 34 and 34, respectively) and were cultivated in selective medium, and the cultivable yeasts were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anthropometric and biochemical measures were also evaluated. RESULTS: Eutrophic, overweight and obese groups presented concentrations of 1.6, 2.16 and 2.19 log10  colony-forming units g-1 yeast, respectively. Ascomycota and Basidiomycota were the two identified phyla. At the genus level, Candida spp. showed a relatively high prevalence, and 10 different species were detected: Candida glabrata, Candida orthopsilosis, Candida lambica, Candida kefyr, Candida albicans, Candida krusei, Candida valida, Candida parapsilosis, Candida utilis and Candida humilis (with relative abundances of 71.72%, 5.05%, 21.21%, 6.06%, 29.29%, 27.27%, 8.08%, 16.16%, 1.01% and 2.02%, respectively). CONCLUSIONS: The obese group presented a higher prevalence of Candida albicans. Furthermore, Candida albicans, Candida kefyr and Rhodotorula mucilaginosa showed a high positive correlation with obesity, weight gain and fat mass and showed a negative correlation with high-density lipoprotein and lean mass, parameters related to weight loss.

4.
Arq. bras. cardiol ; 116(1): 56-65, Jan. 2021. tab, graf
Artículo en Portugués | LILACS-Express | LILACS | ID: biblio-1152975

RESUMEN

Resumo Fundamento A hipertensão arterial (HTA) representa um grande fator de risco de morbidade e mortalidade cardiovascular. Ainda não se sabe que mecanismos moleculares específicos estão associados ao desenvolvimento de hipertensão essencial. Objetivo Neste trabalho, analisamos a associação entre expressão mRNA de monócito LRP1, expressão de proteína LRP1, e espessura íntima-média de carótida (EIMC) de pacientes com hipertensão essencial. Métodos A expressão mRNA de monócito LRP1 e os níveis de proteína e EIMC foram quantificados em 200 indivíduos mexicanos, sendo 91 normotensos (NT) e 109 hipertensos (HT) A significância estatística foi definida em p < 0,05. Resultados O grupo de pacientes HT tinha EIMC maior altamente significativa em comparação com os pacientes NT (p = 0,002), e isso está relacionado ao aumento na expressão mRNA de LRP1 (6,54 versus. 2,87) (p = 0,002) e expressão de proteína LRP1 (17,83 versus 6,25), respectivamente (p = 0,001). Essas diferenças foram mantidas mesmo quando dividimos nossos grupos de estudo, levando em consideração apenas aqueles que apresentavam dislipidemia na expressão de mRNA (p = 0,041) e de proteínas (p < 0,001). Também se identificou que a indução de LRP1 mediada por LRP1 em monócitos em de maneira dependente de dose e tempo, com diferença significativa em NT versus HT (0,195 ± 0,09 versus 0,226 ± 0,12, p = 0,046). Conclusão Foi encontrado um aumento em EIMC em indivíduos com hipertensão, associada a expressões de proteína LRP1 e mRNA mais altas em monócitos, independente da presença de dislipidemia em pacientes HT. Esses resultados que a upregulation de LRP1 em monócitos de pacientes hipertensos mexicanos poderia estar envolvida na diminuição da EIMC. (Arq Bras Cardiol. 2021; 116(1):56-65)


Abstract Background Arterial hypertension (HTA) represents a major risk factor for cardiovascular morbidity and mortality. It is not yet known which specific molecular mechanisms are associated with the development of essential hypertension. Objective In this study, we analyzed the association between LRP1 monocyte mRNA expression, LRP1 protein expression, and carotid intima media thickness (cIMT) of patients with essential hypertension. Methods The LRP1 monocyte mRNA expression and protein levels and cIMT were quantified in 200 Mexican subjects, 91 normotensive (NT) and 109 hypertensive (HT). Statistical significance was defined as p < 0.05. Results HT patients group had highly significant greater cIMT as compared to NT patients (p=0.002) and this correlated with an increase in the expression of LRP1 mRNA expression (6.54 vs. 2.87) (p = 0.002) and LRP1 protein expression (17.83 vs. 6.25), respectively (p = 0.001). These differences were maintained even when we divided our study groups, taking into account only those who presented dyslipidemia in both, mRNA (p = 0.041) and proteins expression (p < 0.001). It was also found that Ang II mediated LRP1 induction on monocytes in a dose and time dependent manner with significant difference in NT vs. HT (0.195 ± 0.09 vs. 0.226 ± 0.12, p = 0.046). Conclusion An increase in cIMT was found in subjects with hypertension, associated with higher mRNA and LRP1 protein expressions in monocytes, irrespective of the presence of dyslipidemias in HT patients. These results suggest that LRP1 upregulation in monocytes from Mexican hypertensive patients could be involved in the increased cIMT. (Arq Bras Cardiol. 2021; 116(1):56-65)

5.
Am J Trop Med Hyg ; 103(3): 1125-1128, 2020 09.
Artículo en Inglés | MEDLINE | ID: mdl-32602434

RESUMEN

Subarachnoid neurocysticercosis (SANCC) is a severe and progressive brain infection with Taenia solium. We performed a pilot study of noninvasive screening for SANCC in two endemic villages in northern Peru using a urine antigen screen followed by brain magnetic resonance imaging for participants with elevated levels of antigen. Among the 978 participants screened, we identified eight individuals with SANCC, many of whom were asymptomatic. This represents a minimum prevalence of 0.8% of SANCC, a level higher than expected based on prior studies, and a positive predictive value of 62% for our novel urine screening test. Future studies should confirm whether early detection and management improve clinical outcomes.


Asunto(s)
Antígenos Helmínticos/orina , Neurocisticercosis/diagnóstico por imagen , Espacio Subaracnoideo/inmunología , Taenia solium/inmunología , Teniasis/diagnóstico por imagen , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Animales , Enfermedades Asintomáticas , Encéfalo/diagnóstico por imagen , Encéfalo/parasitología , Niño , Femenino , Humanos , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Neurocisticercosis/epidemiología , Neurocisticercosis/parasitología , Perú/epidemiología , Proyectos Piloto , Teniasis/epidemiología , Teniasis/parasitología , Adulto Joven
6.
Oxid Med Cell Longev ; 2020: 5392454, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32273946

RESUMEN

Patients with the Loeys-Dietz syndrome (LDS) have mutations in the TGF-ßR1, TGF-ßR2, and SMAD3 genes. However, little is known about the redox homeostasis in the thoracic aortic aneurysms (TAA) they develop. Here, we evaluate the oxidant/antioxidant profile in the TAA tissue from LDS patients and compare it with that in nondamaged aortic tissue from control (C) subjects. We evaluate the enzymatic activities of glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR), catalase (CAT), superoxide dismutase (SOD) isoforms, and thioredoxin reductase (TrxR). We also analyze some antioxidants from a nonenzymatic system such as selenium (Se), glutathione (GSH), and total antioxidant capacity (TAC). Oxidative stress markers such as lipid peroxidation and carbonylation, as well as xanthine oxidase (ORX) and nuclear factor erythroid 2-related factor 2 (Nrf2) expressions, were also evaluated. TAA from LDS patients showed a decrease in GSH, Se, TAC, GPx, GST, CAT, and TrxR. The SOD activity and ORX expressions were increased, but the Nrf2 expression was decreased. The results suggest that the redox homeostasis is altered in the TAA from LDS patients, favoring ROS overproduction that contributes to the decrease in GSH and TAC and leads to LPO and carbonylation. The decrease in Se and Nrf2 alters the activity and/or expression of some antioxidant enzymes, thus favoring a positive feedback oxidative background that contributes to the TAA formation.


Asunto(s)
Antioxidantes/metabolismo , Aneurisma de la Aorta Torácica/sangre , Síndrome de Loeys-Dietz/sangre , Oxidantes/metabolismo , Adolescente , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Mutación , Adulto Joven
7.
Mol Biol Rep ; 47(2): 1321-1329, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31853766

RESUMEN

ATP-binding cassette membrane transporters (ABC), functions as an outflow facilitator of phospholipids and cellular cholesterol, playing an important role in the development of atherosclerosis and arterial hypertension. ABC's transporters could post-transcriptionally regulated by miRs. Evaluate the association in the transporters ABCA1 and ABCG1 with the expression of miR-33a and miR-144 and the carotid intima media thickness (cIMT) in patients with essential arterial hypertension. The miR-33a-5p, miR-144-3p and mRNA ABCA1 and ABCG1 expression in monocytes from Mexican hypertensive patients were examined by RT-PCR. The miR-33a and miR-144 expression in monocytes and mRNA ABCA1 and ABCG1 from Mexican hypertensive patients were examined by RT-PCR. This study involved 84 subjects (42 normotensive subjects and 42 patients with essential hypertension). Our study revealed that miR-33a expression (p = 0.001) and miR-144 (p = 0.985) were up-regulated, meanwhile, ABCA1 and ABCG1 transporters were down-regulated (p = 0.007 and p = 0.550 respectively) in hypertensive patients compared with the control group. The trend remains for miR33a/ABCA1 in presence of cIMT. Moreover, an inverse correlation was found with the expression levels of ABCA1 and ABCG1 as well as in HDL-C with miR-33a and miR-144. Our results showed an increase in the expression of miR-33a and miR-144 and an inverse correlation in their target ABCA1 and ABCG1; it may be associated with essential arterial hypertension in patients with cIMT and as consequence for atheromatous plaque.


Asunto(s)
Transportador 1 de Casete de Unión a ATP/genética , Transportador de Casetes de Unión a ATP, Subfamilia G, Miembro 1/genética , Grosor Intima-Media Carotídeo , Regulación de la Expresión Génica , Estudios de Asociación Genética , Hipertensión/genética , MicroARNs/genética , Angiotensinas/metabolismo , Índice de Masa Corporal , Dislipidemias/genética , Femenino , Humanos , Masculino , MicroARNs/metabolismo , Persona de Mediana Edad
8.
Int J Surg Case Rep ; 66: 43-47, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31790951

RESUMEN

INTRODUCTION: A lymphocele is defined as an atypical collection of lymphatic fluid not bordered by distinct epithelial lining, which develops in anatomic compartments. Inguinal lymphocele is a common complication of surgery in the inguinal region, with an incidence ranging from 1 to 87 %. This report summarizes the management of an inguinal lymphocele post excision of an inguinal mass. PRESENTATION OF CASE: Herein, we present a case in which an inguinal lymphocele developed four months post excision of an inguinal mass, which was later diagnosed as lymphoma and treated with iliac chain radiation therapy. The conservative treatment with lymphocele drainage, compression dressing and prophylactic antibiotic was initially implemented. As the patient did not respond to conservative treatment, the surgical strategy consisted of excision of lymphocele associated with lymphatic ducts ligation. Negative pressure wound therapy completed the treatment. DISCUSSION: Non-surgical treatment of lymphocele developing from lymphatic injure during groin dissection is not rarely unsuccessful. Surgical options include lymphocele excision with either ligation of the lymphatic ducts or lymphatic-venous shunts between afferent lymphatics and the collateral branch of great saphenous vein. Vacuum-assisted closure therapy assists the wound healing process by increasing blood flow, removing inhibiting factors of wound healing and decreasing the bacterial count. CONCLUSION: Inguinal lymphocele that is not reabsorbed or does not resolve with conservative treatment should be surgically treated. Lymphocele excision with ligation of lymphatic vessels, followed by negative pressure wound therapy appears to be a safe and effective approach.

9.
Artículo en Inglés | MEDLINE | ID: mdl-31816847

RESUMEN

Aim: To investigate the relation between polymorphisms in the interleukin 10 (IL)-10, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-ß and interferon (IFN)-γ genes and Takayasu's arteritis in the Mexican population. Methods: A case-control study was performed to investigate the associations of IL-10, TNF-α, TGF-ß and IFN-γ polymorphisms in a sample of 52 Takayasu's arteritis patients, diagnosed according to the criteria of the American College of Rheumatology and EULAR PRINTO criteria when the patients were under 18 years of age; 60 clinically healthy unrelated Mexican individuals by the 5' exonuclease TaqMan polymerase chain reaction. Polymorphic haplotypes were constructed after linkage disequilibrium analysis. Results: Significant differences were not found in the distribution for genotype and allele frequencies of the polymorphisms studied between healthy controls and Takayasu´s arteritis patients. Likewise, significant associations were not detected in the haplotype analysis with the different genes studied. Conclusions: These findings suggest that the polymorphisms in IL-10, TNF-α, TGF-ß and IFN-γ might not contribute to the susceptibility of Takayasu´s arteritis in the Mexican population.


Asunto(s)
Citocinas/genética , Polimorfismo Genético , Arteritis de Takayasu/genética , Adolescente , Adulto , Anciano , Estudios de Casos y Controles , Niño , Femenino , Frecuencia de los Genes , Genotipo , Humanos , Masculino , México , Persona de Mediana Edad , Adulto Joven
10.
Parasit Vectors ; 12(1): 352, 2019 Jul 16.
Artículo en Inglés | MEDLINE | ID: mdl-31311596

RESUMEN

BACKGROUND: Taenia solium (cysticercosis) is a parasitic cestode that is endemic in rural populations where open defecation is common and free-roaming pigs have access to human feces. The purpose of this study was to examine the roaming patterns of free-range pigs, and identify areas where T. solium transmission could occur via contact with human feces. We did this by using GPS trackers to log the movement of 108 pigs in three villages of northern Peru. Pigs were tracked for approximately six days each and tracking was repeated in the rainy and dry seasons. Maps of pig ranges were analyzed for size, distance from home, land type and contact with human defecation sites, which were assessed in a community-wide defecation survey. RESULTS: Consistent with prior GPS studies and spatial analyses, we found that the majority of pigs remained close to home during the tracking period and had contact with human feces in their home areas: pigs spent a median of 79% (IQR: 61-90%) of their active roaming time within 50 m of their homes and a median of 60% of their contact with open defecation within 100 m of home. Extended away-from-home roaming was predominately observed during the rainy season; overall, home range areas were 61% larger during the rainy season compared to the dry season (95% CI: 41-73%). Both home range size and contact with open defecation sites showed substantial variation between villages, and contact with open defecation sites was more frequent among pigs with larger home ranges and pigs living in higher density areas of their village. CONCLUSIONS: Our study builds upon prior work showing that pigs predominately roam and have contact with human feces within 50-100 m of the home, and that T. solium transmission is most likely to occur in these concentrated areas of contact. This finding, therefore, supports control strategies that target treatment resources to these areas of increased transmission. Our finding of a seasonal trend in roaming ranges may be useful for control programs relying on pig interventions, and in the field of transmission modeling, which require precise estimates of pig behavior and risk.


Asunto(s)
Cisticercosis/veterinaria , Defecación , Heces/parasitología , Estaciones del Año , Enfermedades de los Porcinos/transmisión , Animales , Conducta Animal , Cisticercosis/epidemiología , Cisticercosis/transmisión , Femenino , Sistemas de Información Geográfica , Humanos , Masculino , Movimiento , Perú/epidemiología , Factores de Riesgo , Población Rural , Análisis Espacial , Porcinos/parasitología , Enfermedades de los Porcinos/epidemiología , Enfermedades de los Porcinos/parasitología , Taenia solium/aislamiento & purificación
11.
Am J Trop Med Hyg ; 100(6): 1490-1493, 2019 06.
Artículo en Inglés | MEDLINE | ID: mdl-30938282

RESUMEN

Cysticercosis, caused by Taenia solium, is a neglected disease that causes preventable epilepsy. We conducted an experiential learning workshop in northern Peru to educate community members on T. solium transmission and motivate participation in community-led prevention and control. The workshop included presentation of local economic and epidemiologic data, followed by hands-on participation in pig dissection, group discussion of the T. solium life cycle, and viewing of eggs and nascent tapeworms with light microscopes. Among heads of household, we used community survey data to compare knowledge of the three-stage parasite life cycle at baseline and 2 months postworkshop. Knowledge of the life cycle increased significantly after the workshop, with greater gains for workshop attendees than non-attendees. Prior knowledge and workshop attendance were significant predictors of postworkshop knowledge. The use of local evidence and experiential learning positively affected knowledge of T. solium transmission, laying the foundation for subsequent community-engaged control efforts.


Asunto(s)
Cisticercosis/patología , Cisticercosis/parasitología , Educación en Salud , Enfermedades de los Porcinos/parasitología , Taenia solium , Animales , Niño , Femenino , Humanos , Masculino , Población Rural , Porcinos , Zoonosis
12.
Environ Health ; 18(1): 33, 2019 04 11.
Artículo en Inglés | MEDLINE | ID: mdl-30975138

RESUMEN

BACKGROUND: Adequate maternal thyroxine (T4) concentrations during the first half of pregnancy are fundamental to the embryo's or fetus' neural development. Organophosphate pesticides (OP) can act as thyroid disruptors and genetic polymorphisms for paraoxonase 1 (PON1), an enzyme that detoxifies OP, could be involved in individual's susceptibility to them. We assessed the association between para-occupational exposure to pesticides, including OP, during pregnancy and maternal hypothyroxinemia, as well as the potential genetic susceptibility conferred by PON1 polymorphisms. METHODS: We analyzed information from 381 healthy pregnant women (< 17 gestational weeks), who lived in a floricultural region of Mexico where pesticides, including OP, are routinely used. Women who were para-occupationally exposed to pesticides were those whose partner had an occupation involving contact with these products. Thyroid-Stimulating Hormone (TSH) and free T4 concentrations were determined using ELISA, and hypothyroxinemia was defined as free T4 concentrations <0.76 ng/dL. PON1192QR, PON155LM and PON1-108CT polymorphisms were determined through Polymerase Chain Reaction (PCR). The association between para-occupational exposure and genetic polymorphisms and hypothyroxinemia was estimated using logistic regression models. RESULTS: One hundred and sixty two women (42.52%) were classified as para-occupationally exposed to pesticides. Hypothyroxinemia prevalence was 54%, and it was not significantly associated with pesticide para-occupational exposure (OR: 1.21 95% CI 0.75-1.94). Independently of para-occupational exposure, the likelihood of hypothyroxinemia was higher among women who were carriers of PON155MM than in those with PON155LL genotype (OR MM vs LL: 3.03; 95%CI 1.62, 5.70). PON1192 RR (OR RR vs QQ: 1.72; 95%CI 0.93, 3.17) and PON1-108TT (OR TT vs CC: 1.60; 95%CI 0.90, 2.70) genotypes were marginally associated with hypothyroxinemia. No significant interaction was observed between pesticides para-occupational exposure and PON1 polymorphisms. CONCLUSIONS: These results suggest that PON1 polymorphisms could affect thyroid function during pregnancy in women living in areas where pesticides, including OP, are routinely used. Low exposure variability in this population, could be a possible explanation for the lack of association between para-occupational exposure and thyroid function.


Asunto(s)
Arildialquilfosfatasa/genética , Exposición Materna , Compuestos Organofosforados , Plaguicidas , Tirotropina/sangre , Tiroxina/sangre , Adolescente , Adulto , Agricultura , Femenino , Humanos , México , Polimorfismo Genético , Embarazo , Adulto Joven
13.
Gene ; 689: 69-75, 2019 Mar 20.
Artículo en Inglés | MEDLINE | ID: mdl-30529100

RESUMEN

MTHFR is a key enzyme in folate metabolism. Some genetic polymorphisms code for a less efficient enzyme, increasing serum concentrations of homocysteine. This has been associated with inadequate feto-maternal circulation and increased risk of spontaneous abortion. Paroxonase 1 (PON1) is a multifunctional enzyme that can detoxify homocysteine through its homocysteine thiolactonase activity. We evaluate the association between MTHFR 677 C>T polymorphisms and non-recurrent spontaneous abortion and its interaction with PON1 polymorphisms involved in homocysteine metabolism in women living in floricultural areas in Mexico. Sociodemographic, reproductive history, folic acid consumption during pregnancy and environmental exposure data of 264 women who had been pregnant sometime during the 10 years prior to study enrolment were collected. MTHFR 677 C>T, PON1 192Q>R and PON1 55L>M genotypes were determined by PCR amplification. Information on pregnancy outcome and maternal genotypes was obtained for 484 pregnancies: 34 non-recurrent spontaneous abortions (gestational age < 20 weeks) and 450 controls. GEE models were used to evaluate the association between MTHFR polymorphism and non-recurrent spontaneous abortion, and its interaction with PON1 polymorphisms. After adjusting for potential confounders, no significant association was found between the MTHFR 677 C>T maternal polymorphism and non-recurrent spontaneous abortion (OR CT vs CC= 0.39, 95% CI: 0.14-1.05; OR TT vs CC = 0.63, 95% CI: 1.22-1.80). No interactions with PON1 192Q>R or PON1 55L>M polymorphisms were identified (p for interaction = 0.88 and 0.41, respectively). PON1 55L>M maternal polymorphism was associated with higher risk of spontaneous abortion (OR LM/MM vs LL = 4.14, 95% CI: 1.49-11.54). Our results do not demonstrate an interaction between the MTHFR 677 C>T and PON1 192Q>R or PON1 55L>M maternal polymorphisms neither an independent association of MTHFR 677 C>T polymorphism with non-recurrent spontaneous abortion, whereas PON1 55LM/MM maternal genotype increase the odds of this event.


Asunto(s)
Aborto Espontáneo/genética , Arildialquilfosfatasa/genética , Metilenotetrahidrofolato Reductasa (NADPH2)/genética , Polimorfismo de Nucleótido Simple , Aborto Espontáneo/epidemiología , Adolescente , Adulto , Estudios de Casos y Controles , Estudios Transversales , Femenino , Estudios de Asociación Genética , Predisposición Genética a la Enfermedad , Humanos , México/epidemiología , Embarazo , Adulto Joven
14.
Inflamm Res ; 68(3): 195-201, 2019 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-30470857

RESUMEN

OBJECTIVES: Takayasu's arteritis (TA) represents a rare autoimmune disease (AD) characterized by systemic vasculitis that primarily affects large arteries, especially the aorta and the aortic arch and its main branches. Genetic components in TA are largely unknown. PTPN22 is a susceptibility loci for different ADs; however, the role of different PTPN22 single-nucleotide polymorphisms (SNPs) in the susceptibility to TA is not clear. METHODS: We evaluated the PTPN22 R620W (C1858T), R263Q (G788A), and - 123G/C SNPs in a group of patients with TA and in healthy individuals from Mexico. Our study included 111 patients with TA and 314 healthy individuals. Genotyping was performed with the 5' exonuclease (TaqMan®) assay. RESULTS: Our data showed that the PTPN22 R620W polymorphism is a risk factor for TA (CC vs. CT: OR 4.3, p = 0.002, and C vs. T: OR 4.1, p = 0.003); however, the PTPN22 R263Q and - 1123G/C polymorphisms are not associated with this AD. In addition, the PTPN22 CGT haplotype, which carries the minor allele of the PTPN22 C1858T variant, was also associated with TA susceptibility. CONCLUSION: This is the first report documenting an association between PTPN22 R620W and TA.


Asunto(s)
Proteína Tirosina Fosfatasa no Receptora Tipo 22/genética , Arteritis de Takayasu/genética , Adulto , Femenino , Predisposición Genética a la Enfermedad , Genotipo , Humanos , Masculino , México , Persona de Mediana Edad , Polimorfismo de Nucleótido Simple , Factores de Riesgo
15.
Am J Trop Med Hyg ; 100(2): 314-322, 2019 02.
Artículo en Inglés | MEDLINE | ID: mdl-30560769

RESUMEN

The pork tapeworm, Taenia solium, is among the leading causes of preventable epilepsy in the world and is common in rural areas of developing countries where sanitation is limited and pigs have access to human feces. Prior studies in rural villages of Peru have observed clusters of T. solium cysticercosis among pigs that live near human tapeworm carriers. Such spatial analyses, however, have been limited by incomplete participation and substandard diagnostic tests. In this study, we evaluated the association between necropsy-confirmed cysticercosis in pigs and their distance to T. solium tapeworm carriers in six villages in northern Peru. A total of six (1.4%) tapeworm carriers were detected using copro-antigen enzyme-linked immunosorbent assay and seven of 10 (70%) pigs belonging to the tapeworm carriers were found with viable cyst infection on necropsy. This was significantly greater than the prevalence of viable cyst infection among pigs living < 500 m (11%) and > 500 m (0.5%) from a tapeworm carrier (P < 0.001 for distance trend). Similar statistically significant prevalence gradients were observed after adjustment for possible confounders and for other pig-level outcomes including infection with > 10 viable cysts, degenerated cyst infection, and serological outcomes. This investigation confirms that porcine cysticercosis clusters strongly around tapeworm carriers in endemic rural regions of northern Peru and supports interventions that target these hotspots.


Asunto(s)
Anticuerpos Antihelmínticos/sangre , Cisticercosis/epidemiología , Enfermedades de los Porcinos/epidemiología , Taenia solium/inmunología , Adulto , Animales , Autopsia , Análisis por Conglomerados , Cisticercosis/inmunología , Cisticercosis/transmisión , Heces/parasitología , Femenino , Humanos , Incidencia , Masculino , Persona de Mediana Edad , Perú/epidemiología , Prevalencia , Población Rural , Análisis Espacial , Porcinos , Enfermedades de los Porcinos/inmunología , Enfermedades de los Porcinos/transmisión , Taenia solium/aislamiento & purificación
16.
Am J Trop Med Hyg ; 100(1): 140-142, 2019 01.
Artículo en Inglés | MEDLINE | ID: mdl-30457096

RESUMEN

Reintroduction of Taenia solium into a region in Peru where it had been eliminated prompted evaluation of the possibility of reintroduction from an urban reservoir of taeniasis. In a cross-sectional study of an adjacent urban area, we found low prevalence of taeniasis (4/1,621; 0.25%), suggesting minimal risk of parasite reintroduction into rural areas through this route.


Asunto(s)
Neurocisticercosis/epidemiología , Teniasis/epidemiología , Población Urbana , Adulto , Anciano , Animales , Niño , Estudios Transversales , Femenino , Humanos , Masculino , Persona de Mediana Edad , Perú/epidemiología , Prevalencia , Factores de Riesgo , Taenia solium/aislamiento & purificación , Teniasis/complicaciones , Teniasis/transmisión , Adulto Joven
17.
Clin Appl Thromb Hemost ; 25: 1076029618780344, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-29916259

RESUMEN

The activity of the enzyme methylenetetrahydrofolate reductase (MTHFR) determines homocysteine (Hcy) levels, and polymorphisms in its gene affect the activity of the enzyme. Changes in the enzyme's activity may lead to a higher susceptibility to develop arterial and venous thromboembolic disease. The aim was to analyze the relationship between the C677T and A1298C polymorphisms of MTHFR, Hcy levels, and prothrombotic biomarkers in pulmonary embolism (PE) and acute myocardial ischemia (AMI). Clinical files of patients with thromboembolic diseases having complete data and whose doctor had requested an assay to determine the polymorphisms of the MTHFR gene, Hcy levels, and prothrombotic biomarkers were studied to search for the correlation between mutations of the MTHFR gene and Hcy levels in the different diseases. We included 334 files: 158 were from women and 176 from men (51 [19 SD] years). Sixty-three percent have had thrombosis, 8% AMI, and 31% PE. Patients with thrombosis had elevated frequency of the C677T polymorphism. The CC genotype was higher than the TT genotype ( P = .003) and CT versus the TT ( P = .009). In patients with PE, the CC genotype was higher than the TT genotype ( P = .038). Pulmonary embolism with massive and submassive events had predominant genotypes 677 TT ( P = .003) and the AA 1298 ( P = .017). Elevated Hcy levels in the presence of the T allele in the C677T gene and of the A allele in the A1298C gene are associated with AMI and massive and submassive PE.


Asunto(s)
Biomarcadores/metabolismo , Enfermedad de la Arteria Coronaria/genética , Homocisteína/metabolismo , Metilenotetrahidrofolato Reductasa (NADPH2)/genética , Polimorfismo de Nucleótido Simple/genética , Embolia Pulmonar/genética , Femenino , Predisposición Genética a la Enfermedad , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos
18.
J Digit Imaging ; 32(1): 19-29, 2019 02.
Artículo en Inglés | MEDLINE | ID: mdl-30097747

RESUMEN

We present a methodology for the automatic recognition of negated findings in radiological reports considering morphological, syntactic, and semantic information. In order to achieve this goal, a series of rules for processing lexical and syntactic information was elaborated. This required development of an electronic dictionary of medical terminology and informatics grammars. Pertinent information for the assembly of the specialized dictionary was extracted from the ontology SNOMED CT and a medical dictionary (RANM, 2012). Likewise, a general language dictionary was also included. Lexicon-Grammar (LG), proposed by Gross (1975; Cahiers de l'institut de linguistique de Louvain, 24. 23-41 1998), was used to set up the database, which allowed an exhaustive description of the argument structure of predicates projected by lexical units. Computational framework was carried out with NooJ, a free software developed by Silberztein (Silberztein and Noo 2018, 2016), which has various utilities for treating natural language, such as morphological and syntactic grammar, as well as dictionaries. This methodology was compared with a Spanish version of NegEx (Chapman et al. Journal of Biomedical Informatics, 34(5):301-310 2001; Stricker 2016). Results show that there are minimal differences in favor of the algorithm developed using NooJ, but the quality and specificity of the data improves if lexical-grammatical information is added.


Asunto(s)
Procesamiento Automatizado de Datos/métodos , Registros Electrónicos de Salud , Lingüística/métodos , Radiología/métodos , Terminología como Asunto , Humanos , Lenguaje
19.
J Hum Hypertens ; 32(10): 681-690, 2018 10.
Artículo en Inglés | MEDLINE | ID: mdl-30232400

RESUMEN

miR-33a has been described as a key regulator in the initiation and progression of atherosclerosis. However, its role in arterial hypertension (HTA) has not been elucidated. Therefore, the aim of this study was to determine the association between the expression of miR-33a (5p and 3p) and the carotid intima-media thickness (cIMT) in samples of monocytes and serum from hypertensive patients. The miR-33a-5p and miR-33a-3p expression in monocytes and serum from Mexican hypertensive patients were examined by RT-PCR. This study involved 84 subjects (42 normotensive subjects and 42 patients with essential hypertension). Our study revealed that miR-33a-5p expression was significantly upregulated in the monocytes of hypertensive patients compared with the control group (p = 0.001), while miR-33a-3p was significantly downregulated (p = 0.013). miR-33a-5p upregulation [OR: 5.53, 95% CI: 2.01-15.20; p = 0.001], as well as miR-33a-3p downregulation [OR: 3.32, 95% CI: 1.45-7.60; p = 0.004] in monocytes, was associated with an increased risk of developing hypertension. In addition, miR-33a-5p upregulation in hypertensive patients was associated with an increased risk of presenting cIMT [OR: 5.99, 95% CI: 1.10-32.85; p = 0.039]. Moreover, we found no significant differences in the expression of both strands of miR-33a in serum of our patients. Our results showed an upregulation of miR-33a-5p and downregulation of miR-33a-3p in monocytes, these data are associated with cIMT, which could be a risk factor for the development of hypertension. In addition, upregulation of miR-33a-5p in monocytes from Mexican hypertensive patients could be involved in the development of atherosclerosis.


Asunto(s)
Grosor Intima-Media Carotídeo , Hipertensión/sangre , MicroARNs/metabolismo , Anciano , Angiotensina I/sangre , Angiotensina II/sangre , Estudios de Casos y Controles , Femenino , Humanos , Hipertensión/diagnóstico por imagen , Masculino , Persona de Mediana Edad , Monocitos/metabolismo , Fragmentos de Péptidos/sangre , Proteínas de Unión a los Elementos Reguladores de Esteroles/metabolismo
20.
Sex., salud soc. (Rio J.) ; (29): 340-372, mayo-ago. 2018. tab
Artículo en Portugués | LILACS | ID: biblio-979354

RESUMEN

Resumo: Este artigo parte de pesquisa que articula etnografia, inquérito comportamental e estudo de prevalência do HIV entre gays, outros HSH, travestis e mulheres transexuais em dois distritos administrativos na região central da cidade de São Paulo. Focaliza a produção articulada da vulnerabilidade para o HIV, de ações de prevenção, das atribuições de "risco" e de espaços da cidade - seus territórios e fronteiras -, considerando que tal produção é perpassada por relações de poder que se constituem mutuamente. Tais fronteiras, que delimitam diferentes lugares, frequências e tipos de estabelecimentos, mas também podem dificultar ou impedir a oferta de ações de prevenção, são vistas como sendo ao mesmo tempo simbólicas e materiais. Interessa-nos pensar: 1) como os distritos estudados se diferenciam entre si e internamente em termos de perfil socioeconômico, identidades, práticas sexuais e protetivas de seus frequentadores; 2) como a identidade sociossexual é atribuída diferentemente aos dois distritos estudados; 3) como a atribuição da ideia de "risco" para a infecção pelo HIV é acionada de diferentes modos em relação aos dois distritos; 4) como a distribuição socioespacial das pessoas, dos estabelecimentos, das identidades e do "risco" produz fronteiras no espaço da cidade, intersectando variadas relações sociais de poder ou marcadores sociais da diferença.


Abstract: This article is part of a research that articulates ethnography, behavioral investigation and HIV prevalence study among gay men, other MSM, travestis and transsexual women in two administrative districts in the central region of the city of São Paulo. It focus on the articulated production of HIV vulnerability, prevention actions, "risk" assignments and city spaces - their territories and borders - considering that such production is permeated by mutually constitutive power relations. Such boundaries, which delimit different places, frequencies and types of establishments, but also hamper prevention actions from being offered, are seen as both symbolic and material. Analysis is centered on: 1) how the studied districts differ among themselves and internally in terms of socioeconomic profile, identities, sexual and protective practices of its people; 2) how the socio-sexual identity is attributed differently in the two studied districts; 3) how the idea of "risk" for HIV infection is attributed in different ways in relation to the two districts; 4) how the socio-spatial distribution of people, establishments, identities and "risk" produces boundaries in the city space, intersecting various social relations of power or social markers of difference.


Resumen: Este artículo parte de un estudio que articula etnografía, investigación comportamental y estudio de prevalencia de VIH entre gays, otros HSH, travestis y mujeres transexuales en dos distritos administrativos en la región central de la Ciudad de São Paulo. Se focaliza la producción articulada de la vulnerabilidad para el VIH, de acciones de prevención, de las atribuciones de "riesgo" y de espacios de la ciudad - sus territorios y fronteras -, considerando que tal producción es atravesada por relaciones de poder que se constituyen mutuamente. Tales fronteras, que delimitan diferentes lugares, frecuencias y tipos de establecimientos, pero también pueden dificultar o impedir la oferta de acciones de prevención, son vistas como siendo al mismo tiempo simbólicas y materiales. Nos interesa pensar: 1) como los distritos estudiados se diferencian entre si e internamente por perfil socioeconómico, identidades, prácticas sexuales y protección de sus frecuentadores; 2) como la identidad sociosexual es atribuida diferentemente a los dos distritos estudiados; 3) como la atribución de la idea de "riesgo" para la infección por VIH es accionada de diferentes modos en relación a los dos distritos; 4) como la distribución socioespacial de las personas, de los establecimientos, de las identidades y del "riesgo" producen fronteras en el espacio de la ciudad, intersectando variadas relaciones sociales de poder o marcadores sociales de la diferencia.


Asunto(s)
Humanos , Masculino , Femenino , Homosexualidad , Riesgo , VIH , Sexualidad , Vulnerabilidad Social , Transexualidad , Brasil , Investigación Cualitativa , Prevención de Enfermedades
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