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1.
BMC Oral Health ; 21(1): 1, 2021 Jan 02.
Artículo en Inglés | MEDLINE | ID: mdl-33388028

RESUMEN

BACKGROUND: Technology advancement has rising in the past decade and brought several innovations and improvements. In dentistry, this advances provided more comfortable and quick procedures to both the patient and the dental surgeon, generating less predictability in the final result. Several techniques has been developed for the preparation of surgical guides aiming at the optimization of surgical procedures. The present study aimed to evaluate the reproducibility and precision of two types of surgical guides obtained using 3D printing and milling methods. METHODS: A virtual model was developed that allowed the virtual design of milled (n = 10) or 3D printed (n = 10) surgical guides. The surgical guides were digitally oriented and overlapped on the virtual model. For the milling guides, the Sirona Dentsply system was used, while the 3D printing guides were produced using EnvisionTEC's Perfactory P4K Life Series 3D printer and E-Guide Tint, a biocompatible Class I certified material. The precision and trueness of each group during overlap were assessed. The data were analyzed with GraphPad software using the Kolmogorov-Smirnov test for normality and Student's t test for the variables. RESULTS: The Kolmogorov-Smirnov test showed a normal distribution of the data. Comparisons between groups showed no statistically significant differences for trueness (p = 0.529) or precision (p = 0.3021). However, a significant difference was observed in the standard deviation of mismatches regarding accuracy from the master model (p < 0.0001). CONCLUSIONS: Within the limits of this study, surgical guides fabricated by milling or prototyped processes achieved similar results.

2.
J Int Acad Periodontol ; 23(1): 57-64, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-33512341

RESUMEN

AIMS: To estimate the change in the alveolar ridge by means of a new alveolar ridge preservation (ARP) approach, using an anodized titanium foil (Tseal) associated with a bovine bone graft (BBG) by cone-beam computerized tomography. MATERIALS AND METHODS: Sixteen patients, each presenting one hopeless tooth, were selected and these teeth were carefully extracted. The alveolar socket was filled with BBG and the Tseal was trimmed and adapted to the bone crest. The primary outcome variable was the change in the alveolar dimension (AD) measurements between baseline (T1) and 6 months (T2) 1mm below the palatal bone. RESULTS: Imaging assessment of AD demonstrated a decreased value in all subjects. The absolute rate and percentage of absorption between T1 and T2 time point showed statistically significant differences. The mean AD varied from 9.88 ± 2.04 mm (T1) to 8.85 ± 1.92 mm (T2). On average, this ARP procedure maintained 89.55% ± 6.11% of the distance of between the buccal and palatal wall. No differences were observed between the maxilla and mandible (p greater than 0.05). CONCLUSION: The application of a bovine bone graft covered with Tseal resulted in clinically important horizontal preservation of the alveolar ridge at 6 months after extraction.

3.
J Int Acad Periodontol ; 22(4): 231-235, 2020 10 01.
Artículo en Inglés | MEDLINE | ID: mdl-32980835

RESUMEN

AIMS: This study evaluated the mechanical properties of mandibular bone tissue retrieved from postmenopausal women under alendronate treatment. METHODS: Twenty postmenopausal women were divided into two groups: healthy postmenopausal subjects (control group) and osteoporotic subjects treated with alendronate (alendronate group). Mandibular bone samples were retrieved with a trephine bur at the time of dental implant placement and fixed in 4% formalin. Samples were processed for hard tissue histology, and the bone surface was analyzed for nanohardness measurement. Nanohardness and elastic modulus were evaluated by using a Berkovich tip with elastic modulus of 1.016x106 MPa, Poisson coefficient of 0.3, and a load of 100 mN. Each cycle was configured with a load time of 18 seconds (speed of 1 mN/second), the discharge time of 18 seconds, and a rest time of 5 seconds during indentation at a depth of 10 µm. RESULTS: The control group presented the highest values for nanohardness and elastic modulus (p less than 0.05) in relation to the osteoporotic subjects. CONCLUSIONS: Within the limitations of the study, it can be concluded that treatment with alendronate negatively influenced the mechanical properties of mandibular bone in postmenopausal women by reducing bone nanohardness and elastic modulus.


Asunto(s)
Alendronato , Posmenopausia , Módulo de Elasticidad , Femenino , Dureza , Humanos , Mandíbula
4.
Clin Oral Investig ; 2020 Jun 27.
Artículo en Inglés | MEDLINE | ID: mdl-32594309

RESUMEN

BACKGROUND AND OBJECTIVES: Treg and TH17 cells influence the inflammatory process in periodontal diseases and could also play in a similar pattern, an essential role in immune-inflammatory mechanisms involved in the destruction of the peri-implant tissues, peri-implantitis. Therefore, this study evaluated the levels of RORγT and FOXP3 gene expression in subjects with peri-implantitis and healthy peri-implant tissues. METHODS: A total of 35 subjects with implant-supported restorations in both diseased and healthy clinical conditions (n = 15 healthy; n = 20 peri-implantitis) were included in this study. Peri-implantitis was defined as probing depth > 5 mm, bleeding on probing and/or suppuration, and peri-implant bone loss >4 mm. Peri-implant tissue biopsies were collected for analysis of the mRNA, RORγT, and FOXP3 expression levels. The samples were submitted to total RNA extraction, treatment with DNAse, and cDNA synthesis. Subsequently, real-time PCR reaction was performed to evaluate the levels of RORγT and FOXP3 gene expression to the reference gene. These were analyzed by the non-parametric Mann-Whitney method with a level of significance of 5%. RESULTS: Higher gene expression levels of the transcription factors RORγT and FOXP3 were detected in the tissues affected by peri-implantitis when compared with healthy tissues (p < 0.05). CONCLUSIONS: The present study demonstrated the possible existence of a hybrid TH17-Treg profile, based on the gene expression of transcription factors inducing differentiation of these cells. Further studies must be designed to gain a better understanding of the immunological mechanisms involved in the pathogenesis of peri-implantitis. CLINICAL RELEVANCE: The levels of RORγT and FOXP3 transcription factors that were linked to cells with the FOXP3+RORγT+ phenotype could be used as a predictor of peri-implantitis progression.

5.
J Nutr Biochem ; 77: 108301, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-31825817

RESUMEN

We sought to evaluate the effects of magnesium (Mg) intake deficiency on bone metabolism in rats with induced periodontal disease (PD). Holtzman rats were randomly divided into two groups: Control - animals fed a standard diet and test - animals fed a diet with 90% Mg deficiency. After 60 days on the diets, all animals received ligature on the lower left first molars to induce PD. Animals were euthanized after 30 days following ligature placement. Blood and urine were collected for determination of serum concentrations of Mg, calcium, osteocalcin (OCN), alkaline phosphatase and parathyroid hormone (PTH) by enzyme-linked immunosorbent assay, and the urinary concentration of deoxypyridinoline (DPD). Systemic bone mineral density (BMD), bone volume and architectural bone parameters were evaluated by micro-CT in L4 lumbar vertebrae and mandible. Tartrate-resistant acid phosphatase staining and immunohistochemical (IHC) analysis of inducible nitric oxide synthase (iNOS), Runt-related transcription factor 2 (RUNX2), CD86, CD80, proliferating cell nuclear antigen, vascular endothelial growth factor, OCN and osteopontin were investigated. Reverse-transcription polymerase chain reaction was employed to assess mRNA expression of receptor-activator of nuclear factor-kB ligand, osteoprotegerin (OPG) and interleukin (IL)-6. Mg deficiency was associated with higher concentrations of PTH and DPD, and significant decrease on both systemic and mandibular BMD, as well as greater severity of alveolar and trabecular bone loss. Significant increase in osteoclasts was observed in the test group with PD. IHC analysis showed significant increase in the expression of iNOS and decreased expression of OCN and RUNX2. Increased IL-6 mRNA and decreased OPG mRNA expressions were evidenced in the test group with PD. Mg deficiency caused systemic effects indicative of altered bone metabolism in the vertebrae and affected both immune and stromal cells, aggravating inflammatory bone resorption in the ligature-induced model of periodontitis.

6.
Clin Oral Investig ; 23(4): 1733-1744, 2019 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-30159806

RESUMEN

OBJECTIVES: The aim of this study was to investigate bone turnover alterations after alendronate (ALD) withdrawal and its influence on dental implants osseointegration. MATERIALS AND METHODS: Seventy female Wistar rats were randomly divided in 2 groups that received on day 0 either placebo (control group-CTL; n = 10) or 1 mg/kg sodium alendronate (ALD; n = 60) once a week for 4 months. At day 120, ALD treatment was suspended for 50 animals. Then, a titanium implant was placed in the left tibia of each rat that were randomly allocated in five subgroups of ten animals each, according to the period of evaluation: day 0 (INT-0), day 7 (INT-7), day 14 (INT-14), day 28 (INT-28), and day 45 (INT-45) after ALD withdrawal. CTL group and a group that received ALD until the end of the experimental period (non-interrupted group-non-INT; n = 10) underwent implant placement on day 120. Animals were euthanized 28 days after implant surgery. Bone mineral density (BMD) of femur and lumbar vertebrae were evaluated by DXA, biochemical markers of bone turnover were analyzed by ELISA, and bone histomorphometry was performed to measure bone-to-implant contact (BIC) and bone area fraction occupancy (BAFO). RESULTS: All groups receiving ALD showed higher BMD values when compared to CTL group, which were maintained after its withdrawal. Decreased concentrations in all bone turnover markers were observed in the non-INT group, and in the groups in which ALD was discontinued compared to the CTL group. The non-INT group showed lower %BIC and notably changes in bone quality, which was persistent after drug withdrawal. CONCLUSION: Collectively, the findings of this study demonstrated that ALD therapy decreased bone turnover and impaired bone quality and quantity around dental implants, and that its discontinuation did not reverse these findings. CLINICAL RELEVANCE: The severe suppression of bone turnover caused by the prolonged use of ALD may alter the capacity of bone tissue to integrate with the implant threads impairing the osseointegration process.


Asunto(s)
Alendronato/administración & dosificación , Remodelación Ósea , Implantes Dentales , Oseointegración , Animales , Densidad Ósea , Femenino , Distribución Aleatoria , Ratas , Ratas Wistar , Tibia , Titanio
7.
J Periodontal Res ; 54(2): 174-179, 2019 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-30362297

RESUMEN

BACKGROUND AND OBJECTIVE: Little is known about the correspondence of cytokines detected among the different biological matrices used in periodontal research and whether locally-detected cytokine mRNA can also be identified at the translated protein level. The aims of this study were to compare: (a) the detection of classic cytokines at protein level in corresponding samples of serum, gingival tissue and gingival crevicular fluid (GCF) in subjects with periodontitis and (b) the detection of cytokines at protein level in GCF and tissue with their detection at mRNA level in tissue. MATERIAL AND METHODS: Protein concentrations of IL-1ß, IL-6 and TNF-α were measured in corresponding samples of GCF, gingival tissue and serum from 125 subjects with chronic periodontitis by multiplex immunoassay. The mRNA levels of these cytokines were evaluated in the same gingival tissue by qPCR. RESULTS: The mean protein concentrations of IL-1ß and TNF-α were higher in tissue and GCF than in serum (P < 0.05). The mean protein concentration of IL-6 was higher in tissue, followed by serum and GCF, respectively (P < 0.05). Using serum as the gold standard, detection of IL-1ß in GCF and IL-6 in tissue had the highest sensitivity (100%), while detection of IL-6 in the GCF had the lowest sensitivity (77.9%). Using tissue as the gold standard, the detection sensitivities of IL-1ß, IL-6 and TNF-α were 93.6%, 78.4% and 94.2%, respectively, in GCF. Using mRNA as the gold standard, the detection of IL-1ß and IL-6 at the protein level in tissue (100.0%) had the highest sensitivity, while the detection of IL-6 in GCF (77.9%) had the lowest sensitivity. CONCLUSION: Tissue and GCF exhibited overall higher protein concentrations of cytokines than serum. GCF presented good reliability for identifying the target cytokines at protein level, when compared with detecting tissue cytokines at protein level. GCF and tissue presented high sensitivities for detecting cytokines at the protein level, when compared with detecting tissue cytokines at the mRNA level. The sensitivity for the identification of cytokines in the GCF depended on the target cytokine, where IL-6 was the least likely cytokine to be detected in this matrix.


Asunto(s)
Periodontitis Crónica/genética , Periodontitis Crónica/metabolismo , Encía/metabolismo , Líquido del Surco Gingival/metabolismo , Mediadores de Inflamación/metabolismo , Interleucina-1beta/genética , Interleucina-1beta/metabolismo , Interleucina-6/genética , Interleucina-6/metabolismo , ARN Mensajero/metabolismo , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/metabolismo , Adulto , Anciano , Anciano de 80 o más Años , Periodontitis Crónica/sangre , Femenino , Humanos , Mediadores de Inflamación/sangre , Interleucina-1beta/sangre , Interleucina-6/sangre , Masculino , Persona de Mediana Edad , ARN Mensajero/sangre , Factor de Necrosis Tumoral alfa/sangre
8.
Int J Oral Maxillofac Implants ; 33(6): 1339-1344, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-30427965

RESUMEN

PURPOSE: The All-on-4 treatment concept has been shown to be an effective clinical procedure; however, to date, no studies have analyzed the subgingival microbiota present in these restorations. The purpose of this study was to evaluate the microbial profile of the subgingival biofilm around dental implants placed in the All-on-4 protocol and compare the microbial profile around axial and tilted implants. MATERIALS AND METHODS: Fourteen subjects treated by the All-on-4 concept were evaluated clinically and microbiologically. Subgingival biofilm was collected from each patient, and the amount of 40 species of bacteria was assessed using the checkerboard DNA-DNA hybridization technique. RESULTS: The results for the indices of probing depth (PD), bleeding on probing, marginal bleeding, and visible plaque were 2.32 mm, 46%, 60%, and 57%, respectively. Tilted implants presented a significantly higher mean PD and Plaque Index compared with axial implants (P < .05). Fusobacterium nucleatum ssp vincentii, Veillonella parvula, and Fusobacterium nucleatum ssp polymorphum were found in higher levels; however, no difference in the microbial composition was observed between tilted and axial implants (P > .05). Tilted implants presented statistically higher mean levels for the orange complex in relation to the axial implants (P < .05). CONCLUSION: Despite the clinical success rate of the All-on-4 protocol, the subgingival biofilm of tilted implants presented a higher proportion for the orange complex pathogens in comparison to axial implants. These data could suggest that subjects with this modality of implant-supported restoration must be aware that they need a more rigorous maintenance protocol.


Asunto(s)
Bacterias/clasificación , Biopelículas , Implantación Dental Endoósea/microbiología , Implantes Dentales/microbiología , Prótesis Dental de Soporte Implantado/microbiología , Encía/microbiología , Arcada Edéntula/cirugía , Fenómenos Fisiológicos Bacterianos , Índice de Placa Dental , Femenino , Estudios de Seguimiento , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Resultado del Tratamiento
9.
Rev. odontol. UNESP (Online) ; 47(3): 143-148, maio-jun. 2018. tab, graf
Artículo en Inglés | LILACS-Express | LILACS | ID: biblio-961516

RESUMEN

Abstract Introduction Temporomandibular disorders (TMD) can compromise masticatory performance due to the presence of pain, muscular dysfunction and limitation of mandibular movements. Substantial improvements in TMD pain can be achieved through therapeutic approaches including education and self-care. Objective The aim of the present study was to investigate the impact of these treatment modalities in the masticatory performance of women with painful TMD. Material and method Fifty-two women were randomly divided into three groups: Control Group (CG); Education Group (received education about TMD) (EG); Self-Care and Education Group (received education about TMD and instructions self-care therapies) (SEG). The education instructions about TMD and self-care therapies were transmitted through a video and printed illustrative material. The masticatory performance was evaluated through the sieves method ​​with natural tested foods in the evaluation periods: baseline evaluation, 30 days after and 60 days of follow-up. Data were analyzed and used with a one-way ANOVA parametric test for mean comparison of CG, EG and SEG groups (α = 0.05). Result The masticatory performance of the participants was similar, irrespective of the proposed treatments, at baseline (p=.604), 30 days (p=.450) and 60 days (p=.669). Conclusions The results indicated that treatment with educational and self-care therapies over a period of 60 days had no impact on the masticatory performance of women with painful TMD.


Resumo Introdução As disfunções temporomandibulares (DTM) podem comprometer a capacidade mastigatória devido à presença de dor, disfunção muscular e limitação dos movimentos mandibulares. Melhorias substanciais da dor relacionada à DTM podem ser alcançadas através de abordagens terapêuticas incluindo a educação e os autocuidados. Objetivo O objetivo do presente estudo foi investigar o impacto dessas modalidades de tratamento no desempenho mastigatório de mulheres com DTM dolorosa. Material e método Cinquenta e duas mulheres foram divididas aleatoriamente em três grupos: Grupo Controle (CG); Grupo Educação (receberam instruções sobre DTM) (EG); Grupo de Autocuidado e Educação (receberam instruções sobre DTM e instruções sobre terapias de autocuidado) (SEG). As instruções de educação sobre DTM e terapias de autocuidado foram transmitidas através de vídeo e material ilustrativo impresso. O desempenho mastigatório foi avaliado através do método dos tamises com alimentos testes naturais nos períodos de avaliação: atendimento inicial, 30 dias após e ao fim de 60 dias de acompanhamento. Os dados obtidos, em porcentagem, foram analisados e foi utilizado o teste paramétrico ANOVA one-way para comparação das médias dos grupos CG, EG e SEG (α= 0,05). Resultado O desempenho mastigatório dos participantes foi semelhante, independentemente dos tratamentos, no atendimento inicial (p=0,604), após 30 dias (p=0,450) e após 60 dias (p=0,669). Conclusão Os resultados indicam que o tratamento com terapias educativas e de autocuidado durante um período de 60 dias não teve impacto no desempenho mastigatório das mulheres com DTM dolorosa.

10.
Int J Oral Maxillofac Implants ; 33(3): 565-570, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29763494

RESUMEN

PURPOSE: To evaluate the gene expression levels of semaphorins 3A, 3B, 4A, and 4D in both healthy and diseased implants. MATERIALS AND METHODS: Subjects with peri-implantitis presented clinical attachment loss, probing depth ≥ 5 mm, bleeding on probing and/or suppuration, and radiographic bone loss > 4 mm. Peri-implant tissue biopsy specimens were sampled for analysis of the mRNA expression levels for semaphorins 3A, 3B, 4A, and 4D. A real-time polymerase chain reaction was performed, and the gene expression levels of semaphorins in relation to the housekeeping gene were analyzed by using the nonparametric Mann-Whitney test (P < .05). RESULTS: Thirty-five subjects (16 men, 19 women; mean age: 54.12 ± 2.34 years) with implant-supported restorations, using screw-shaped dental implants with internal or external hexagon were enrolled in this study. Higher levels of semaphorins 3A and 4D were detected in the peri-implantitis compared with the healthy tissues (P = .0011 and P = .0404, respectively), whereas Sem4A levels were significantly higher in the control group (P < .0001). Differences between groups in the expression levels of Sem3B were not significant. CONCLUSION: Advanced peri-implantitis lesions showed higher levels of gene expression for Sem3A and Sem4D and lower levels of Sem4A in comparison to tissues obtained from a healthy dental implant.


Asunto(s)
Antígenos CD/genética , Regulación de la Expresión Génica/fisiología , Glicoproteínas de Membrana/genética , Periimplantitis/genética , Semaforina-3A/genética , Semaforinas/genética , Estudios de Casos y Controles , Implantes Dentales , Femenino , Humanos , Masculino , Persona de Mediana Edad , Periimplantitis/cirugía , ARN Mensajero/genética , Reacción en Cadena en Tiempo Real de la Polimerasa , Supuración
11.
J Oral Implantol ; 43(4): 297-301, 2017 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-28628350

RESUMEN

The lack of compatible prosthetic components can be a complication during oral rehabilitation using outdated implants. The aim of the present clinical report was to describe an alternative technique for the fabrication of a maxillary implant-supported overdenture in a patient with 20-year-old dental implants using castable spherical patterns and ball attachments. The patient had been wearing a relined bar/clip overdenture in the mandible on 4 external-hexagon dental implants and a relined complete denture in the maxilla on 4 internal-hexagon implants due to abutment screw fracture inside of the implants, losing the attachment system. The remaining maxillary dental implants did not possess attachments compatible with current systems due to configuration changes by the manufacturer in the dental implant's platform and the components over time. Therefore, castable spherical patterns and cast ball attachments were used to fabricate a maxillary implant-supported overdenture. The mandible rehabilitation was performed using 4 osseointegrated dental implants with a fixed implant-supported prosthesis. The use of cast ball attachments on the maxillary dental implants avoided invasive procedures on the remaining implants. Considering the lack of available compatible prosthetic components for the osseointegrated implants, this technique was considered a viable and satisfactory treatment option.


Asunto(s)
Prótesis Dental de Soporte Implantado/instrumentación , Prótesis Dental de Soporte Implantado/métodos , Retención de Dentadura/métodos , Prótesis de Recubrimiento , Arcada Edéntula/cirugía , Diseño de Dentadura , Humanos , Mandíbula/cirugía , Maxilar/cirugía
12.
Clin Oral Implants Res ; 28(7): e10-e15, 2017 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-27062688

RESUMEN

OBJECTIVES: The mRNA expression profiles of IL-23/Th17 and the Treg-associated cytokine TGF-ß in peri-implantitis are currently under research. This study characterized the IL-17, IL-23, and TGF-ß gene expression levels in healthy and diseased peri-implant tissues and correlated these data with radiographic bone loss. MATERIAL AND METHODS: Peri-implant soft tissues from 40 subjects (20 healthy controls with mean age of 59.4 ± 6.3, and 20 with peri-implantitis with mean age of 56.6 ± 5.5) were enrolled in this study, and real-time PCR (RT-PCR) was used to define the profile of IL-17, IL-23, and TGF-ß gene expression. RESULTS: Higher levels of TGF-ß mRNA were observed in biopsies taken from healthy controls, and the IL-23 mRNA levels were significantly increased in the peri-implantitis group (P < 0.0001). No differences in IL-17 mRNA levels were observed between the two groups (P > 0.05). CONCLUSIONS: Data presented in this report demonstrated a predominant Th17 response in peri-implantitis subjects based on the higher levels of IL-23 and lower levels of TGF-ß detected.


Asunto(s)
Expresión Génica , Interleucina-17/genética , Interleucina-23/genética , Periimplantitis/genética , Factor de Crecimiento Transformador beta/genética , Biopsia , Estudios de Casos y Controles , Femenino , Humanos , Masculino , Persona de Mediana Edad , ARN Mensajero/genética , Reacción en Cadena en Tiempo Real de la Polimerasa
13.
J Prosthet Dent ; 116(5): 749-755, 2016 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-27236596

RESUMEN

STATEMENT OF PROBLEM: Limited mandibular movements are one of the most important signs of temporomandibular disorders (TMDs) and may cause functional difficulties. PURPOSE: The purpose of this double-blind, randomized clinical trial was to evaluate the effect of treatment with only educational or education associated with self-care therapies on the pattern of mandibular movements of women with chronic painful TMDs. MATERIAL AND METHODS: Forty-two women were selected and randomly divided into 3 groups, control group (CG, n=13), education group (EG, n=16), and education and self-care group (ESG, n=13), according to the sequence of treatment they received. A kinesiograph device recorded mandibular movements during maximum mouth opening and mastication at baseline (T0) and at 30-day (T1) and 60-day (T2) follow-up. Kinesiographic data were statistically analyzed using 1-way ANOVA, followed by the Bonferroni test for multiple comparisons of means (α=.05). RESULTS: The ESG group demonstrated an improvement in the maximum vertical opening (MVO= 5.1 ±3.4 mm; P=.012) and anteroposterior mandibular movement (MAM) during maximum opening (7.4 ±9.5; P=.019), significantly higher than that of the EG (MVO=1.8 ±3.5 mm; MAM=0.8 ±5.0 mm) and the CG (MVO=0.9 ±3.8 mm; MAM=0.8 ±4.4 mm) after 30 days of follow-up. Moreover, at T1, vertical mandibular movement during mastication was significantly higher in the ESG group (17.4 ±1.7 mm) than in the EG group (15.0 ±2.8, P=.027). No significant differences were found between the women who received treatment with educational and self-care therapies for 60 days and the women who received this treatment for 30 days. CONCLUSIONS: In the short-term, education and self-care treatment positively influenced the mandibular movement pattern of women with chronic painful TMDs.


Asunto(s)
Educación del Paciente como Asunto , Autocuidado , Trastornos de la Articulación Temporomandibular/terapia , Adulto , Método Doble Ciego , Femenino , Humanos , Mandíbula , Masticación , Movimiento , Rango del Movimiento Articular
14.
J Prosthodont ; 25(6): 485-8, 2016 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-26916386

RESUMEN

PURPOSE: To assess the effect of brushing with different solutions on the abrasion resistance of two types of acrylic resin teeth. MATERIALS AND METHODS: Maxillary premolars from two types of acrylic teeth (Biotone and Biotone IPN) were divided into six groups (n = 12), according to the solution used during brushing: distilled water (control), coconut soap, or dentifrice. A mechanical brushing machine was used to simulate approximately 1 year of brushing (11,000 strokes). The weight loss (WL) of teeth was obtained from the difference between the initial (IW) and final weight (FW) of each specimen, and the mean of percentage of weight loss (PWL) was calculated for each group. Data were analyzed using the Kruskal-Wallis test, followed by Bonferroni's post-test comparison (α = 0.05). RESULTS: A statistically significant difference was found for the factor solution (p < 0.001). Brushing using dentifrice caused the highest values of weight loss (-0.50%), in comparison with the groups brushed with coconut soap (0.00%) or distilled water (0.00%). CONCLUSIONS: For both types of artificial teeth, brushing with dentifrice produced higher abrasion than brushing with coconut soap or water.


Asunto(s)
Diente Artificial , Cepillado Dental , Pastas de Dientes , Dentífricos , Humanos , Abrasión de los Dientes
15.
J Prosthodont ; 25(7): 576-579, 2016 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-26489039

RESUMEN

PURPOSE: To evaluate the bond strength between two types of acrylic resin teeth and a microwave denture base resin after immersion in disinfectant solutions for 180 days. MATERIALS AND METHODS: Eighty specimens made of acrylic resin teeth (Biotone and Biotone IPN) attached to a microwave polymerized denture base resin (Nature-Cryl MC) were divided into eight groups (n = 10) according to the treatment (distilled water-control, 2% chlorhexidine digluconate, 1% sodium hypochlorite and sodium perborate solution-Corega Tabs). The shear strength tests (MPa) were carried out using a universal testing machine with a 0.5 mm/min speed. Data analysis was performed using ANOVA and multiple comparison Student-Newman-Keuls post hoc test (α = 0.05). RESULTS: Biotone IPN showed similar results among the groups (distilled water, 8.25 ± 1.81 MPa; chlorhexidine, 7.81 ± 3.34 MPa; hypochlorite, 7.75 ± 3.72 MPa; and Corega Tabs, 7.58 ± 2.27 MPa, whereas Biotone showed significantly lower shear bond strength values for the groups immersed in Corega Tabs (5.25 ± 3.27 MPa) and chlorhexidine (6.08 ± 2.35 MPa). CONCLUSIONS: Soaking the dentures in 1% sodium hypochlorite could be recommended as a disinfectant solution for dentures fabricated with conventional acrylic resin denture teeth and microwave denture base resin. For dentures fabricated with IPN teeth and microwave denture base resin, all the soaking solutions evaluated in this study could be suggested to denture wearers.


Asunto(s)
Recubrimiento Dental Adhesivo , Bases para Dentadura , Microondas , Diente Artificial , Resinas Acrílicas , Desinfectantes , Humanos , Ensayo de Materiales , Propiedades de Superficie
16.
J Prosthet Dent ; 115(4): 462-8, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-26548879

RESUMEN

STATEMENT OF PROBLEM: The difficulty of removing denture adhesive is a common problem reported by users of these products. PURPOSE: The purpose of this clinical study was to investigate the effectiveness of different cleaning protocols for removing a denture adhesive (DA) and the influence on the oral microbiota. MATERIAL AND METHODS: Twenty participants wearing well-fitting complete dentures were instructed to use a denture adhesive 3 times a day during a 4-week trial, divided into 4 stages: (A) control-3 daily denture brushings using water at ambient temperature, (B)-3 daily denture brushings using water at ambient temperature plus coconut soap, (C)-3 daily denture brushings using water at ambient temperature plus dentifrice; (D)-3 daily denture brushings using water at ambient temperature combined with immersion in sodium perborate solution for 5 minutes before nocturnal sleep. After each 1-week stage, saliva specimens were collected. A dye was used to display and quantify the remaining DA on the internal surface of the maxillary dentures as a percentage. For microbiological analysis, the saliva was diluted and plated onto Petri dishes containing a nonselective culture medium and Candida spp culture media. After the incubation period, Candida species were identified and the number of colony forming units (CFU/mL) was calculated. RESULTS: A significant difference was found among the 4 cleaning methods for the quantification of remaining DA (Friedman, P=.036). Brushing the dentures with coconut soap, dentifrice, or water combined with immersion in sodium perborate solution was more effective in removing DA than brushing with only water. The cleaning methods did not influence the quantification of microorganisms in general or Candida albicans and other Candida species in particular. CONCLUSIONS: Brushing the dentures with coconut soap, dentifrice, or water combined with immersion in sodium perborate solution was more effective for removing cream-type denture adhesive than brushing with only water.


Asunto(s)
Adhesivos/química , Biopelículas/crecimiento & desarrollo , Limpiadores de Dentadura , Boca/microbiología , Carga Bacteriana , Recuento de Colonia Microbiana , Estudios Cruzados , Cementos Dentales , Humanos , Microbiota , Jabones , Propiedades de Superficie , Cepillado Dental
17.
Clin Oral Implants Res ; 27(5): 577-82, 2016 May.
Artículo en Inglés | MEDLINE | ID: mdl-26037595

RESUMEN

OBJECTIVES: The present study measured implant stability quotient (ISQ) values at three different time points after surgical procedures to compare whether the stability values differed between implants placed in fresh extraction sockets versus healed alveolar sites. MATERIALS AND METHODS: To measure implant stability, resonance frequency analysis (RFA) was performed in 77 patients (53 women, 24 men) with a total of 120 dental implants. These implants were divided into two groups: Group 1 included 60 implants in healed alveolar sites (22 in the maxilla, 38 in the mandible), and Group 2 included 60 implants in fresh sockets (41 in the maxilla, 19 in the mandible). Implant stability was measured immediately at implant placement (baseline), 90, and 150 days later. Statistical analysis was made using a multivariate regression linear model at implant level (α = 0.05). RESULTS: Overall, the means and standard deviations of the ISQ values were 62.7 ± 7.14 (95% confidence interval [CI], 39-88) at baseline, 70.0 ± 6.22 (95% CI, 46-88) at 90 days, and 73.4 ± 5.84 (95% CI, 58-88) at 150 days. In Group 1, the ISQs ranged between 64.3 ± 6.20 and 75.0 ± 5.69, while in Group 2, presented lower values that ranged between 61.2 ± 8.09 and 71.9 ± 5.99 (P = 0.002). Anatomic location and times periods were the only identified variables with an influence on ISQ values at implant level (P < 0.0001). CONCLUSIONS: The stabilities of the implants placed in the fresh sockets and in healed sites exhibited similar evolutions in ISQ values and thus osseointegration; however, the implants in the healed alveolar sites exhibited superior values at all time points.


Asunto(s)
Proceso Alveolar/cirugía , Implantes Dentales , Oseointegración , Alveolo Dental/cirugía , Femenino , Humanos , Masculino , Mandíbula/cirugía , Maxilar/cirugía
18.
Int J Oral Maxillofac Implants ; 30(6): 1431-6, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26478977

RESUMEN

PURPOSE: The aim of this prospective controlled study was to evaluate the influence of osteopenia on the levels of osteoclastogenesis-related factors in the peri-implant crevicular fluid (PICF) and on the clinical parameters of immediately loaded implants. MATERIALS AND METHODS: This study included 24 patients who received at least two implants in the mandible, with restorations delivered 48 hours after implant placement. Patients were divided into control (n = 11) and osteopenia (n = 13) groups. Seven days after implant placement (baseline) and 4 months after implant placement, PICF samples were obtained, and clinical parameters (Plaque Index, Gingival Index, bleeding on probing, suppuration, probing depths, clinical attachment levels) were measured. A commercially available enzyme-linked immunosorbent assay was used to analyze PICF samples for levels of soluble receptor activator of nuclear factor of κB ligand (sRANKL) and osteoprotegerin (OPG). At the 4-month follow-up visit, the implant-supported restorations were removed and periapical radiographs were acquired to evaluate bone loss around the implants. RESULTS: Eighty-eight immediately loaded implants were included in this study (38 in the control group, 50 in the osteopenia group). The RANKL and OPG levels, the RANKL/OPG ratio, and the clinical parameters were similar between the groups at both time points. However, the levels of these factors in PICF differed significantly between baseline and 4 months after surgery. CONCLUSION: Within the limitations of this short-term study, it can be concluded that osteopenia does not influence the PICF levels of osteoclastogenesis-related factors in immediately loaded implants after 4 months of loading.


Asunto(s)
Enfermedades Óseas Metabólicas/metabolismo , Implantes Dentales , Líquido del Surco Gingival/química , Carga Inmediata del Implante Dental , Osteoclastos/fisiología , Anciano , Pérdida de Hueso Alveolar/metabolismo , Remodelación Ósea/fisiología , Índice de Placa Dental , Ensayo de Inmunoadsorción Enzimática , Femenino , Estudios de Seguimiento , Humanos , Mandíbula/cirugía , Persona de Mediana Edad , Oseointegración/fisiología , Osteoprotegerina/análisis , Índice Periodontal , Posmenopausia/metabolismo , Estudios Prospectivos , Ligando RANK/análisis , Resultado del Tratamiento
19.
Biomed Res Int ; 2015: 159625, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26273590

RESUMEN

The establishment of the subgingival microbiota is dependent on successive colonization of the implant surface by bacterial species. Different implant surface topographies could influence the bacterial adsorption and therefore jeopardize the implant survival. This study evaluated the biofilm formation capacity of five oral streptococci species on two titanium surface topographies. In vitro biofilm formation was induced on 30 titanium discs divided in two groups: sandblasted acid-etched (SAE- n = 15) and as-machined (M- n = 15) surface. The specimens were immersed in sterilized whole human unstimulated saliva and then in fresh bacterial culture with five oral streptococci species: Streptococcus sanguinis, Streptococcus salivarius, Streptococcus mutans, Streptococcus sobrinus, and Streptococcus cricetus. The specimens were fixed and stained and the adsorbed dye was measured. Surface characterization was performed by atomic force and scanning electron microscopy. Surface and microbiologic data were analyzed by Student's t-test and two-way ANOVA, respectively (P < 0.05). S. cricetus, S. mutans, and S. sobrinus exhibited higher biofilm formation and no differences were observed between surfaces analyzed within each species (P > 0.05). S. sanguinis exhibited similar behavior to form biofilm on both implant surface topographies, while S. salivarius showed the lowest ability to form biofilm. It was concluded that biofilm formation on titanium surfaces depends on surface topography and species involved.


Asunto(s)
Biopelículas/crecimiento & desarrollo , Implantes Dentales/microbiología , Saliva/microbiología , Streptococcus/clasificación , Streptococcus/crecimiento & desarrollo , Titanio/química , Materiales Dentales/química , Humanos , Ensayo de Materiales , Especificidad de la Especie , Propiedades de Superficie
20.
World J Orthop ; 6(2): 311-5, 2015 Mar 18.
Artículo en Inglés | MEDLINE | ID: mdl-25793172

RESUMEN

AIM: To assess the failure and bone-to-implant contact rate of dental implants placed on osteoporotic subjects. METHODS: Extensive examination strategies were created to classify studies for this systematic review. MEDLINE (via PubMed) and EMBASE database were examined for studies in English up to and including May 2014. The examination presented a combination of the MeSH words described as follow: "osteoporosis" or "osteopenia" or "estrogen deficiency" AND "implant" or "dental implant" or "osseointegration". Assessment of clinical and/or histological peri-implant conditions in osteoporosis subjects treated with titanium dental implants. The examination included a combination of the MeSH terms described as follow: "osteoporosis" or "osteopenia" or "estrogen deficiency" AND "implant" or "dental implant" or "osseointegration". RESULTS: Of 943 potentially eligible articles, 12 were included in the study. A total of 133 subjects with osteoporosis, 73 subjects diagnosed with osteopenia and 708 healthy subjects were assessed in this systematic review. In these subjects were installed 367, 205, 2981 dental implants in osteoporotic, osteopenic and healthy subjects, respectively. The failure rate of dental implant was 10.9% in osteoporotic subjects, 8.29% in osteopenic and 11.43% in healthy ones. Bone-to-implant contact obtained from retrieved implants ranged between 49.96% to 47.84%, for osteoporosis and non-osteoporotic subjects. CONCLUSION: Osteoporotic subjects presented higher rates of implant loss, however, there is a lower evidence to strengthen or refute the hypothesis that osteoporosis may have detrimental effects on bone healing. Consequently, final conclusions regarding the effect of osteoporosis in dental implant therapy cannot be made at this time. There are no randomized clinical trial accessible for evaluation and the retrospective nature of the evaluated studies shall be taken in account when interpreting this study.

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