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1.
J Oral Implantol ; 45(1): 2-11, 2019 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-30160606

RESUMEN

The purpose of this study was to determine the possible deleterious effects of e-cigarette vapor on osteoblast interaction with dental implant material. Osteoblasts were cultured onto Ti6Al4V titanium implant disks and were then exposed or not to whole cigarette smoke (CS), as well as to nicotine-rich (NR) or nicotine-free (NF) e-vapor for 15 or 30 minutes once a day for 1, 2, or 3 days, after which time various analyses were performed. Osteoblast growth on the titanium implant disks was found to be significantly ( P < .001) reduced following exposure to CS and to the NR and NF e-vapors. Osteoblast attachment to the dental implant material was also dysregulated by CS and the NR and NF e-vapors through a decreased production of adhesion proteins such as F-actin. The effects of CS and e-cigarette vapor on osteoblast growth and attachment were confirmed by reduced alkaline phosphatase (ALP) activity and tissue mineralization. The adverse effects of CS and the NR and NF e-vapors on osteoblast interaction with dental implant material also involved the caspase-3 pathway, as the caspase-3 protein level increased following exposure of the osteoblasts to CS or e-vapor. It should be noted that the adverse effects of CS on osteoblast growth, attachment, ALP, and mineralized degradation were greater than those of the NR and NF e-vapors, although the latter did downregulate osteoblast interaction with the dental implant material. Overall results suggest the need to consider e-cigarettes as a possible contributor to dental implant failure and/or complications.


Asunto(s)
Implantes Dentales , Sistemas Electrónicos de Liberación de Nicotina , Oseointegración/efectos de los fármacos , Osteoblastos , Adhesión Celular , Osteoblastos/efectos de los fármacos , Fumar/efectos adversos , Propiedades de Superficie , Titanio
2.
Rev. bras. cineantropom. desempenho hum ; 20(6): 504-514, Nov.-Dec. 2018. tab
Artículo en Inglés | LILACS | ID: biblio-990558

RESUMEN

Abstract Physical activity measurement with accuracy and precision is extremely important in establishing the dose-response relationship between levels of physical activity and the different outcome possibilities. Subjective methods of measurement, such as proxy-reports have been used as a possibility to check physical activity in children. The aim of this study was to test the Netherlands Physical Activity Questionnaire (NPAQ), valid for Brazilian schoolchildren using physical aerobic fitness as a criterion. The study included 290 children aged 6-10 years from public schools of Itaúna/MG. NPAQ was applied to parents or guardians and children were tested using the Luc Léger test. NPAQ had mean score of 25 for children (25 for boys and 24 for girls) and VO2max was 50.8 ml/kg/min for children (52.6 and 50.2 ml/kg/min for boys and girls, respectively). The Spearmam correlation test showed significant correlation (rsho = 0.146; p = 0.013) between NPAQ and VO2max, but the correlation is weak (Kappa -0.14). The results showed that NPAQ presented poor construct validity for physical activity measurement in a Brazilian schoolchildren sample, based on aerobic physical fitness criteria.


Resumo A mensuração da atividade física com acurácia e precisão é de extrema importância no estabelecimento da relação de dose-resposta entre os níveis de atividade física e as diversas possibilidades de desfecho, sendo um desafio de extrema complexidade. A utilização de métodos subjetivos de mensuração, como os instrumentos proxy-reports, é uma possibilidade de verificação de atividade física em crianças. O objetivo deste estudo foi testar a validade do Netherland Physical Activity Questionnaire (NPAQ) em escolares brasileiros tendo como medida critério a aptidão física aeróbica. Participaram deste estudo 290 crianças de seis a 10 anos de idade, matriculados em escolas públicas do município de Itaúna/MG. Foi aplicado aos responsáveis o NPAQ e as crianças realizaram o teste de Luc Léger. O NPAQ apresentou um escore mediano de 25 para as crianças (25 meninos e 24 meninas) e o VO2máx foi de 50,8 ml/kg/min para as crianças (52,6 e 50,2 ml/kg/min para meninos e meninas, respectivamente). O NPAQ e o VO2máx apresentaram no teste de correlação simples de Spearmam rsho = 0,146 (p = 0,013) correlação significativa, porém fraca (Kappa -0.14). Os resultados permitem concluir que o NPAQ apresentou fraca validade de constructo para mensuração de atividade física em uma amostra de escolares brasileiros, tendo como medida critério a aptidão física aeróbica.


Asunto(s)
Humanos , Niño , Aptitud Física/fisiología , Encuestas y Cuestionarios , Consumo de Oxígeno/fisiología , Prueba de Esfuerzo
3.
Rev. Nutr. (Online) ; 31(5): 467-477, Sept.-Oct. 2018. tab, graf
Artículo en Inglés | LILACS-Express | ID: biblio-1041281

RESUMEN

ABSTRACT Objective To analyze if aerobic capacity is related to Birth Weight and breastfeeding patterns in boys and girls, products of a term pregnancy and normal weight. Methods A representative sample of 230 Brazilian children (6-10 years old), born at term (after 37-weeks' gestation) with normal weight (between 2.5 and 4.0kg). These children performed a Yo-Yo Test to estimate their aerobic capacity and mothers reported their children's Birth Weight and breastfeeding patterns. The Pearson correlation coefficient was used to measure the association between aerobic capacity with Birth Weight and breastfeeding patterns. Results We did not observe any significant associations between aerobic capacity with Birth Weight and breastfeeding time in either sexes (p>0.05). Conclusion These results indicate that aerobic capacity is not related with Birth Weight or breastfeeding time in children born with normal weight and gestational age, suggesting that this complex physiological parameter does not appear to be determined by intrauterine factors that dictate the Birth Weight and breastfeeding patterns in the beginning of life.


RESUMO Objetivo Analisar a relação do desempenho aeróbico com o peso ao nascer e padrões de aleitamento materno na infância de crianças nascidas a termo com peso gestacional adequado. Métodos Duzentas e trinta crianças (6-10 anos), nascidas a termo (pós-37 semanas de gestação) e com peso normal (entre 2,5 e 4kg) participaram do estudo. O desempenho aeróbico foi estimado pelo Yo-Yo Test. O peso ao nascer e os padrões de aleitamento materno foram relatados pelas mães das crianças mediante questionário. A correlação de Pearson foi utilizada para avaliar a relação do desempenho aeróbico com o peso ao nascer e a duração do aleitamento materno. Resultados Não observou-se relação do desempenho aeróbico com o peso ao nascer ou com a duração do aleitamento materno em nenhum dos sexos (p>0,05). Conclusão Os resultados apontam que o desempenho aeróbico não está relacionado ao peso ao nascer e nem à duração do aleitamento materno em crianças nascidas a termo e com peso normal, sugerindo que este parâmetro fisiológico parece não ser associado à fatores intrauterinos que determinam o peso no nascimento e nem ao padrão alimentar no início da vida.

4.
Rev. bras. med. esporte ; 23(2): 93-97, Mar.-Apr. 2017. tab, graf
Artículo en Portugués | LILACS-Express | ID: biblio-843981

RESUMEN

RESUMO Introdução: Diversos são os instrumentos que mensuram atividade física no Brasil. Um deles é o Three Day Physical Activity Recall, porém, até o momento, nenhuma ferramenta foi submetida à adaptação transcultural. Objetivo: Descrever os procedimentos e critérios de escolha do Three Day Physical Activity Recall quanto à equivalência conceitual, semântica e de itens, que resultou na versão brasileira para adolescentes de 10 a 12 anos. Métodos: Foram compilados artigos que continham o instrumento de autorrelato, que tivessem como variáveis de interesse o nível de atividade física e o custo energético. As buscas foram realizadas nas bases de dados BIREME, LILACS, MEDLINE, PubMed, SciELO, Web of Science e SPORTDiscus. Seguiram-se as etapas da adaptação transcultural: dez doutores e 30 membros da população-alvo participaram da avaliação e adequação do constructo à cultura brasileira (equivalência conceitual e de itens) e a versão em português do instrumento seguiu todas as recomendações para equivalência semântica. Resultados: São apresentadas as avaliações dos doutores sobre a versão em português do instrumento, envolvendo questões sobre regionalidade das atividades, dificuldade cognitiva para o autorrelato, instruções, capacidade de mensuração da atividade física e seus domínios. As diferentes versões de tradução e re-tradução do instrumento, com destaque para as modificações solicitadas, assim como a versão final são apresentadas. Conclusões: Tendo sido satisfeitos os pré-requisitos da adaptação transcultural, concluiu-se que o instrumento está adaptado para a população e cultura alvo; entretanto, as qualidades psicométricas, a reprodutibilidade, a validade, o constructo e o critério devem ser testados.


ABSTRACT Introduction: There are several tools that measure physical activity in Brazil. One of them is the Three Day Physical Activity Recall; however, until now, no tool was subjected to cross-cultural adaptation. Objective: To describe the procedures and criteria for selecting the Three Day Physical Activity Recall on the conceptual, semantic and items equivalence, which resulted in the Brazilian version for adolescents from 10 to 12 years. Methods: Articles have been compiled containing the self-report instrument that had as variables of interest the level of physical activity and energy cost. A survey was conducted in the following databases: BIREME, LILACS, MEDLINE, PubMed, SciELO, Web of Science, and SPORTDiscus. The steps of cultural adaptation came next: 10 doctors and 30 members of the target population participated of the evaluation and adjustment of the construct to the Brazilian culture (conceptual and item equivalence) and the Portuguese version of the instrument followed all recommendations for semantic equivalence. Results: Here we present the evaluations of doctors on the Portuguese version of the instrument, involving questions about regionality of activities, cognitive impairment to self-report, instructions, ability to measure physical activity and their domains. The different versions of the translation and retranslation of the tool, highlighting on the changes requested as well as the final version are presented. Conclusions: Since the prerequisites of cultural adaptation have been met, it was concluded that the tool is adapted for the target population and culture; nevertheless, the psychometric qualities, reproducibility and validity, construct and criterion must be tested.


RESUMEN Introducción: Existen varios instrumentos que miden la actividad física en Brasil. Uno de ellos es el Three Day Physical Activity Recall. Sin embargo, hasta el momento, ninguna herramienta se sometió a una adaptación transcultural. Objetivo: Describir los procedimientos y criterios para la selección de Three Day Physical Activity Recall relativos a la equivalencia conceptual, semántica y de ítems, lo que resultó en la versión brasileña para adolescentes de 10 a 12 años. Métodos: Se han compilado artículos que contienen el instrumento de autoinforme que tenían como variable de interés el nivel de actividad y los costos energéticos. Las búsquedas se realizaron en las bases de datos BIREME, LILACS, MEDLINE, PubMed, SciELO, Web of Science y SPORTDiscus. Luego vinieron las etapas de adaptación transcultural: diez doctores y 30 miembros de la población objetivo participaron de la evaluación y la adecuación del constructo a la cultura brasileña (equivalencia conceptual y de ítems) y la versión en portugués del instrumento ha seguido todas las recomendaciones para equivalencia semántica. Resultados: Se presentan las evaluaciones de los doctores sobre la versión en portugués del instrumento, que implica cuestiones sobre la regionalidad de las actividades, dificultad cognitiva para el autoinforme, las instrucciones, la capacidad de medición de la actividad física y sus dominios. Se presentan las diferentes versiones de traducción y re-traducción del instrumento, destacando los cambios solicitados, así como en la versión final. Conclusiones: Después de haber satisfecho los prerrequisitos de adaptación transcultural se concluyó que el instrumento se adapta a la población y cultura objetivo; no obstante, las cualidades psicométricas, la reproducibilidad, la validez, el constructo y el criterio deben ser probados.

5.
Percept Mot Skills ; 122(3): 933-55, 2016 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-27095750

RESUMEN

This study aimed to assess the content validity of a catalog of 76 judo exercises. Two groups of raters comprising 16 judo experts evaluated the following content validity indicators: Clarity of Language, Practical Pertinence, Theoretical Relevance, and the Dimension of each exercise. The results confirmed the content validity of the judo training catalog with indicators showing scores greater than 0.80. These findings suggest that all 76 judo exercises are pertinent, representative of judo training and understandable for judo coaches. Thus, this catalog of judo exercises may help judo coaches in the selection and recording of exercises.


Asunto(s)
Artes Marciales/educación , Artes Marciales/fisiología , Libros de Texto como Asunto/normas , Adulto , Anciano , Humanos , Masculino , Persona de Mediana Edad
6.
Am J Dent ; 28(3): 167-73, 2015 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-26201229

RESUMEN

PURPOSE: To evaluate the in situ effect of fluoride and MDPB-containing adhesives on antibacterial activity around restorations in conditions of high caries risk. METHODS: Bovine enamel and dentin blocks were restored with a fluoride-containing (One-up Bond F Plus - OP) or a MDPB and fluoride-containing adhesive (Clearfil Protect Bond - PB). Volunteers (n = 17) wore an intra-oral appliance containing three enamel and three dentin blocks, aligned side-by-side and restored with OP or PB and one enamel and dentin block (controls). The cariogenic challenge was carried out in two phases of 14 days each. The counts of total streptococci (TM), mutans streptococci (MS) and lactobacilli (LB) were analyzed in the biofilm formed. Cross-sectional microhardness (CSM) and polarized light microscopy (PLM) evaluated caries lesions around the restorations and the demineralization extension. Data obtained by CSM testing was analyzed by Split-Split Plot ANOVA (P < 0.05). PLM and microbiota results were analyzed by Wilcoxon test (P < 0.05). RESULTS: TM and MS counts were highest for the OP enamel restorations, and these presented higher lesion depths than PB in both the enamel and dentin. The CSM in dentin was the lowest at 60 µm from the restoration wall. None of the adhesives prevented demineralization and bacteria growth, but PB reduced the amount of oral pathogens in enamel and demineralization around restorations in enamel and dentin.


Asunto(s)
Antibacterianos/uso terapéutico , Cariostáticos/uso terapéutico , Caries Dental/prevención & control , Materiales Dentales/química , Fluoruros/uso terapéutico , Compuestos de Piridinio/uso terapéutico , Adulto , Anatomía Transversal , Animales , Carga Bacteriana/efectos de los fármacos , Biopelículas/efectos de los fármacos , Bovinos , Estudios Cruzados , Caries Dental/microbiología , Esmalte Dental/efectos de los fármacos , Esmalte Dental/microbiología , Dentina/efectos de los fármacos , Dentina/microbiología , Recubrimientos Dentinarios/química , Método Doble Ciego , Dureza , Humanos , Lactobacillus/efectos de los fármacos , Metacrilatos/química , Microscopía de Polarización , Streptococcus/efectos de los fármacos , Streptococcus mutans/efectos de los fármacos , Desmineralización Dental/microbiología , Desmineralización Dental/prevención & control , Adulto Joven
7.
Artículo en Portugués | LILACS-Express | ID: lil-750857

RESUMEN

Objetivo: verificar a prevalência e os fatores associados ao excesso de peso e ao índice de massa corporal (IMC) de escolares. Métodos: trata-se de estudo transversal, epidemiológico, no qual foram avaliadas 60 crianças de seis a 10 anos de idade, de três escolas públicas de Itaúna-MG. Elas foram classificadas quanto ao IMC/idade e seus pais responderam a questionário para obtenção de dados demográficos, história familiar e pregressa, estilo de vida e frequência alimentar semiquantitativo. Os dados foram avaliados com auxílio dos softwares Excel e SPSS. Foram usados testes estatísticos de regressão logística múltipla e linear múltipla. Resultados: a prevalência de excesso de peso, eutrofia e baixo peso identificada pelo IMC/idade foi de 21,6% (p=13), 76,7% (p=46) e 1,7% (p=1), respectivamente. O excesso de peso foi independentemente associado à obesidade materna (p=0,004). Foi encontrada associaçãodireta entre IMC e obesidade materna (p=0,02), ingestão de pães (p=0,03) e salgadinhos tipo chips (p=0,08) e associação inversa entre IMC e ingestão de iogurte (p=0,007) e sorvete (p=0,09). Conclusão: a prevalência de excesso de peso encontrada na população estudada foi alta, demonstrando a importância de mais atenção a esse aspecto, uma vez que ele pode estar associado a fatores potencialmente modificáveis, como os dietéticos.


Objective: to verify the prevalence and factors associated with overweight and body mass index (BMI) in schoolchildren. Methods: this was a cross sectional epidemiological study in which 60 children from six to 10 years of age were evaluated in three public schools of Itaúna-MG. They were classified by BMI/age, and their parents answered a questionnaire about demographic data, medical and family history, lifestyle, and semi quantitative food frequency. The data were evaluated with the aid of the Excel and SPSS software. Statistical tests for logistic and linear multiple regressions were used. Results: the prevalence of overweight, eutrophy, and low weight, identified by BMI/age, amounted to 21.6% (p = 13),76.7% (p = 46), and 1.7% (p = 1), respectively. Overweight was independently associated with maternal obesity (p = 0.004). A direct association was found between BMI and maternal obesity (p = 0.02), intake of breads (p = 0.03) and snacks such as chips (p = 0.08), and an inverse association was observed between BMI and yogurt (p = 0.007) and ice cream (p = 0.09) intake. Conclusion: the prevalence of overweight found in the studied population was high demonstrating the importance of more attention to this aspect because it can be associated with potentially modifiable factors, such as dietetics.

8.
Rev Paul Pediatr ; 32(3): 208-14, 2014 Sep.
Artículo en Portugués | MEDLINE | ID: mdl-25479851

RESUMEN

OBJECTIVE: To identify the association between both, body mass index and aerobic fitness, with cardiovascular disease risk factors in children. METHODS: Cross-sectional study, carried out in Itaúna-MG, in 2010, with 290 school children ranging from 6 to 10 years-old of both sexes, randomly selected. Children from schools located in the countryside and those with medical restrctions for physical activity were not included. Blood sample was collected after a 12-hour fasting period. Blood pressure, stature and weight were evaluated in accordance with international standards. The following were considered as cardiovascular risk factors: high blood pressure, high total cholesterol, LDL, triglycerides and insulin levels, and low HDL. The statistical analysis included the Spearman's coefficient and the logistic regression, with cardiovascular risk factors as dependent variables. RESULTS: Significant correlations were found, in both sexes, among body mass index and aerobic fitness with most of the cardiovascular risk factors. Children of both sexes with body mass index in the fourth quartile demonstrated increased chances of having high blood insulin and clustering cardiovascular risk factors. Moreover, girls with aerobic fitness in the first quartile also demonstrated increased chances of having high blood insulin and clustering cardiovascular risk factors. CONCLUSION: The significant associations and the increased chances of having cardiovascular risk factors in children with less aerobic fitness and higher levels of body mass index justify the use of these variables for health monitoring in Pediatrics.


Asunto(s)
Índice de Masa Corporal , Enfermedades Cardiovasculares/epidemiología , Aptitud Física , Niño , Estudios Transversales , Femenino , Humanos , Masculino , Factores de Riesgo
9.
Rev. bras. farmacogn ; 24(6): 651-659, Nov-Dec/2014. tab, graf
Artículo en Inglés | LILACS-Express | ID: lil-741839

RESUMEN

In the present investigation we report the antibacterial activity of halistanol sulfate A isolated from the sponge Petromica ciocalyptoides, as well as of rodriguesines A and B isolated from the ascidian Didemnum sp., against the caries etiologic agent Streptococcus mutans. The transcription levels of S. mutans virulence genes gtfB, gtfC and gbpB, as well as of housekeeping genes groEL and 16S, were evaluated by sqRT-PCR analysis of S. mutans planktonic cells. There were no alterations in the expression levels of groEL and 16S after antimicrobial treatment with halistanol sulfate A and with rodriguesines A and B, but the expression of the genes gtfB, gtfC and gbpB was down-regulated. Halistanol sulfate A displayed the most potent antimicrobial effect against S. mutans, with inhibition of biofilm formation and reduction of biofilm-associated gene expression in planktonic cells. Halistanol sulfate A also inhibited the initial oral bacteria colonizers, such as Streptococcus sanguinis, but at much higher concentrations. The results obtained indicate that halistanol sulfate A may be considered a potential scaffold for drug development in Streptococcus mutans antibiofilm therapy, the main etiologic agent of human dental caries. .

10.
PLoS One ; 9(10): e109761, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-25329160

RESUMEN

BACKGROUND AND OBJECTIVE: The immune and infectious alterations occurring in periodontitis have been shown to alter the development and severity of cardiovascular disease. One of these relationships is the translocation of oral bacteria to atheroma plaques, thereby promoting plaque development. Thus, the aim of this study was to assess, by 16s cloning and sequencing, the microbial diversity of the subgingival environment and atheroma plaques of patients concomitantly suffering from periodontitis and obstructive coronary artery atherosclerosis (OCAA). METHODS: Subgingival biofilm and coronary balloons used in percutaneous transluminal coronary angioplasty were collected from 18 subjects presenting with generalized moderate to severe periodontitis and OCAA. DNA was extracted and the gene 16S was amplified, cloned and sequenced. RESULTS: Significant differences in microbial diversity were observed between both environments. While subgingival samples mostly contained the phylum Firmicutes, in coronary balloons, Proteobacteria (p<0.05) was predominant. In addition, the most commonly detected genera in coronary balloons were Acinetobacter, Alloprevotella, Pseudomonas, Enterobacter, Sphingomonas and Moraxella, while in subgingival samples Porphyromonas, Filifactor, Veillonella, Aggregatibacter and Treponema (p<0.05) were found. Interestingly, 17 identical phylotypes were found in atheroma and subgingival samples, indicating possible bacterial translocation between periodontal pockets and coronary arteries. CONCLUSION: Periodontal pockets and atheromatous plaques of cardiovascular disease patients can present similarities in the microbial diversity.


Asunto(s)
Bacterias/aislamiento & purificación , Biodiversidad , Enfermedad de la Arteria Coronaria/complicaciones , Bolsa Periodontal/complicaciones , Bolsa Periodontal/microbiología , Placa Aterosclerótica/complicaciones , Placa Aterosclerótica/microbiología , Bacterias/clasificación , Bacterias/genética , Fenómenos Fisiológicos Bacterianos , Biopelículas , Clonación Molecular , ADN Bacteriano/genética , Femenino , Humanos , Masculino , Persona de Mediana Edad , Filogenia , Análisis de Secuencia de ADN
11.
Rev. paul. pediatr ; 32(3): 208-214, 09/2014. tab
Artículo en Portugués | LILACS | ID: lil-724092

RESUMEN

Objetivo: Identificar a associação do índice de massa corporal e aptidão física aeróbica com fatores de risco de doenças cardiovasculares em crianças. Métodos: Estudo transversal realizado na cidade de Itaúna-MG no ano de 2010 com 290 escolares de 6 a 10 anos de ambos os sexos, aleatoriamente selecionados. Crianças de escolas da zona rural e aquelas com limitações médicas para prática de atividade física não foram incluídas. Coletou-se o sangue após jejum de 12 horas. A pressão arterial, a estatura e o peso foram avaliados segundo padrões internacionais. Foram considerados fatores de risco cardiovascular: hipertensão arterial, colesterol total, LDL, triacilgliceróis e insulinemia elevados e HDL baixo. A análise estatística incluiu a Correlação de Spearman e a Regressão Logística, com os fatores de risco cardiovascular como variáveis dependentes. Resultados: Correlações significativas foram encontradas, nos dois sexos, entre índice de massa corporal e aptidão física aeróbica com a maioria dos fatores de risco cardiovascular. Crianças dos dois sexos com índice de massa corporal acima do percentil 75 apresentaram chances aumentadas para insulinemia alterada e agrupamento de fatores de risco cardiovascular. Meninas com aptidão física aeróbica no primeiro quartil apresentaram chances aumentadas para insulinemia alterada e agrupamento de fatores de risco cardiovascular. Conclusão: As associações significativas e as chances aumentadas para presença de fatores de risco cardiovascular em crianças com menor aptidão física aeróbica e maior índice de massa corporal justificam o uso dessas variáveis no monitoramento da saúde em pediatria...


Objective: To identify the association between both, body mass index and aerobic fitness, with cardiovascular disease risk factors in children. Methods: Cross-sectional study, carried out in Itaúna-MG, in 2010, with 290 school children ranging from 6 to 10 years-old of both sexes, randomly selected. Children from schools located in the countryside and those with medical restrctions for physical activity were not included. Blood sample was collected after a 12-hour fasting period. Blood pressure, stature and weight were evaluated in accordance with international standards. The following were considered as cardiovascular risk factors: high blood pressure, high total cholesterol, LDL, triglycerides and insulin levels, and low HDL. The statistical analysis included the Spearman's coefficient and the logistic regression, with cardiovascular risk factors as dependent variables. Results: Significant correlations were found, in both sexes, among body mass index and aerobic fitness with most of the cardiovascular risk factors. Children of both sexes with body mass index in the fourth quartile demonstrated increased chances of having high blood insulin and clustering cardiovascular risk factors. Moreover, girls with aerobic fitness in the first quartile also demonstrated increased chances of having high blood insulin and clustering cardiovascular risk factors. Conclusion: The significant associations and the increased chances of having cardiovascular risk factors in children with less aerobic fitness and higher levels of body mass index justify the use of these variables for health monitoring in Pediatrics...


Asunto(s)
Humanos , Masculino , Femenino , Niño , Índice de Masa Corporal , Sistema Cardiovascular , Aptitud Física , Factores de Riesgo
12.
Rev. bras. med. esporte ; 20(1): 17-20, Jan-Feb/2014. tab, graf
Artículo en Portugués | LILACS-Express | ID: lil-704721

RESUMEN

INTRODUÇÃO: A estimativa da composição corporal é um campo de estudo importante no prognóstico e diagnóstico de enfermidades degenerativas. OBJETIVO: Analisar a aplicabilidade do Índice de adiposidade corporal (IAC) e o método de dobras cutâneas. MÉTODOS: A amostra foi constituída por 19 adultas jovens com média de idade 24,53 ± 2,65 anos, submetidas à avaliação antropométrica (circunferências e dobras cutâneas) e absorsiometria por dupla emissão de raio X (DXA). Os valores estimados foram comparados ao valor de referência por meio do teste t pareado e pela análise do nível de associação entre os métodos pela correlação de Pearson; o nível de significância foi p < 0,05. RESULTADOS: Não apontaram diferenças significativas entre o percentual de gordura estimado pelo IAC e os valores de referência, porém, tiveram nível de associação moderado (r = 0,627). No entanto, o método de dobras cutâneas apresentou diferença estatística significante (p > 0,05) com nível de associação forte (r = 0,879). CONCLUSÃO: Embora o IAC não apresente diferença para os valores estimados, fica evidente a necessidade de mais estudos sobre a aplicabilidade do método na população brasileira. .


INTRODUCTION: The estimation of body composition is an important study field in the prognosis and diagnosis of degenerative diseases. OBJECTIVE: To analyze the applicability of the Body Adiposity Index (BAI) and the skinfold thickness method. METHODS: The sample consisted of 19 young adult women with a mean age 24.53 ± 2.65 years, who underwent anthropometric measurements (circumferences and skinfold thickness) and dual energy X-ray absorptiometry (DEXA). The estimated values were compared to the reference value by using the paired t test and by analysis of the level of association between the methods through the Pearson correlation. The level of significance was p<0.05. RESULTS: Showed no significant differences between the fat percentage estimated by the BAI and the reference values, however had moderate level of association (r=0.627). Nevertheless, the skinfold thickness method showed statistically significant difference (p>0.05) with a strong association level (r = 0.879). CONCLUSION: Although the BAI does not show difference to the estimated values, it is clear that further studies on the applicability of the method in the Brazilian population are necessary. .


INTRODUCCIÓN: La estimativa de la composición corporal es un campo de estudio importante en el pronóstico y diagnóstico de enfermedades degenerativas. OBJETIVO: Analizar la aplicabilidad del Índice de Adiposidad Corporal (IAC) y el método de pliegues cutáneos. MÉTODOS: La muestra fue constituida por 19 adultas jóvenes con promedio de edad de 24,53 ± 2,65 años, sometidas a evaluación antropométrica (circunferencias y pliegues cutáneos) y absorciometría por doble emisión de rayo X (DXA). Los valores estimados fueron comparados al valor de referencia por medio del test t pareado y por el análisis del nivel de asociación entre los métodos por la correlación de Pearson; el nivel de significancia fue de p < 0,05. RESULTADOS: No apuntaron diferencias significativas entre el porcentual de grasa estimado por el IAC y los valores de referencia, no obstante, tuvieron nivel de asociación moderado (r = 0,627). Por otro lado, el método de pliegues cutáneos presentó diferencia estadística significativa (p > 0,05) con nivel de asociación fuerte (r = 0,879). CONCLUSIÓN: Aunque el IAC no presente diferencia para los valores estimados, queda evidente la necesidad de más estudios sobre la aplicabilidad del método en la población brasileña. .

13.
Braz. j. microbiol ; 43(3): 931-937, July-Sept. 2012. graf, tab
Artículo en Inglés | LILACS | ID: lil-656655

RESUMEN

The aim of the present study was to evaluate the frequency of detection of Mogibacterium timidum in subgingival samples of subjects with generalized aggressive periodontitis (GAgP) and uncontrolled diabetic and non-diabetic subjects with generalized chronic periodontitis (GChP). 48 patients with GAgP, 50 non-diabetic and 39 uncontrolled (glycated hemoglobin >7%) type 2 diabetic subjects with GChP were enrolled in this study. Subgingival biofilm were collected from deep pockets (probing depth > 7 mm). After DNA extraction, M. timidum was detected by Nested Polymerase Chain Reaction and chi-square test was used to data analysis (p>0.05). There were no differences in the frequency of detection of M. timidum between subjects with GAgP (35%) and non-diabetic subjects with GChP (40%) (p>0.05). The frequency of detection of M. timidum was significantly higher in deep pockets of diabetic subjects with GChP (56%) when compared to GAgP (p<0.05), but similar to non-diabetic subjects with GChP (p>0.05). The frequency of detection of M. timidum was higher in subjects GChP presenting uncontrolled type 2 diabetes mellitus, when compared to GAgP subjects.


Asunto(s)
Humanos , Biodiversidad , Placa Dental , Diabetes Mellitus , Eubacterium/crecimiento & desarrollo , Infecciones por Bacterias Grampositivas , Periodontitis , Métodos , Pacientes
14.
Int J Oral Maxillofac Implants ; 27(3): 551-60, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22616048

RESUMEN

PURPOSE: Bacterial leakage along the implant-abutment interface, with consequent species harboring the inner parts of two-part dental implant systems, has been reported in the literature. The aim of this in vitro study was to evaluate bacterial leakage from human saliva to the internal part of the implants along the implant-abutment interface under loaded and unloaded conditions using DNA Checkerboard. MATERIALS AND METHODS: Sixty dental implants--20 each of external-hexagon, internal-hexagon, and Morse cone-connection designs--and their conical abutments were used in this study. Each group was subdivided into two groups of 10 loaded and 10 unloaded implants. The assemblies were immersed in human saliva and either (1) loaded with 500,000 cycles at 120 N (experimental group) or (2) incubated in static conditions for 7 days at 35°C (unloaded control group). RESULTS: Microorganisms were found in the internal surfaces of all types of connections. The Morse cone connection presented the lowest count of microorganisms in both the unloaded and loaded groups. Loaded implants presented with higher counts of microorganisms than unloaded implants for external- and internal-hex connections. CONCLUSION: Bacterial species from human saliva may penetrate along the implant-abutment interface under both unloaded and loaded conditions for all connections evaluated. Morse cone-connection implants showed the lowest counts of microorganisms for both conditions. External- and internal-hex implants showed a higher incidence of bacteria and higher bacterial counts after simulated loading.


Asunto(s)
Coronas , Pilares Dentales/microbiología , Diseño de Implante Dental-Pilar , Implantes Dentales/microbiología , Filtración Dental , Aggregatibacter actinomycetemcomitans/aislamiento & purificación , Recuento de Colonia Microbiana , Humanos , Análisis de Secuencia por Matrices de Oligonucleótidos , Saliva/microbiología , Estadísticas no Paramétricas , Streptococcus gordonii/aislamiento & purificación
15.
Rev. bras. farmacogn ; 22(2): 257-267, Mar.-Apr. 2012. ilus, graf, tab
Artículo en Inglés | LILACS-Express | ID: lil-624669

RESUMEN

In the present investigation we evaluate methods for the isolation and growth of marine-derived fungal strains in artificial media for the production of secondary metabolites. Inoculation of marine macroorganisms fragments in Petri dishes proved to be the most convenient procedure for the isolation of the largest number of strains. Among the growth media used, 3% malt extract showed the best result for strains isolation and growth, and yielded the largest number of strains from marine macroorganisms. The percentage of strains isolated using each of the growth media which yielded cytotoxic and/or antibiotic extracts was in the range of 23-35%, regardless of the growth media used. Further investigation of extracts obtained from different marine-derived fungal strains yielded several bioactive secondary metabolites, among which (E)-4-methoxy-5-(3-methoxybut-1-enyl)-6-methyl-2H-pyran-2-one is a new metabolite isolated from the Penicillium paxilli strain Ma(G)K.

16.
J Periodontol ; 83(8): 988-98, 2012 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-22288485

RESUMEN

BACKGROUND: The aim of the present study is to assess clinical, microbiologic, and immunologic benefits of amoxicillin/metronidazole (AM) when performing full-mouth ultrasonic debridement (FMUD) in generalized aggressive periodontitis (GAgP) treatment. METHODS: Twenty-four GAgP patients were divided into two groups: the FMUD group (n = 12), which received FMUD plus placebo, and the FMUD+AM group (n = 12), which received FMUD and 375 mg amoxicillin plus 250 mg metronidazole for 7 days. The following clinical outcomes were tested: plaque and bleeding on probing indices, pocket probing depth (PD), relative gingival margin position (GMP), and relative clinical attachment level (CAL). Total amount of Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), Tannerella forsythia (Tf), and gingival crevicular fluid (GCF) concentration of interleukin (IL)-10 and IL-1ß were also determined. All clinical, microbiologic, and immunologic parameters were assessed at baseline and at 3 and 6 months post-therapy. The ANOVA/Tukey test was used for statistical analysis (α = 5%). RESULTS: Amoxicillin/metronidazole used as an adjunct to the FMUD protocol added clinical and microbiologic benefits to GAgP treatment (P <0.05). FMUD+AM groups presented an additional PD reduction in initially deep PDs at the 3-month follow-up (3.99 ± 1.16 mm and 3.09 ± 0.78 mm for FMUD+AM and FMUD, respectively; P <0.05), a lower number of residual pockets at the 3- and 6-month follow-ups, and a statistical reduction in amounts of Aa (P <0.05). Analysis of Tf and Pg amounts, as well as IL-10 and IL-1ß GCF concentrations failed to demonstrate a difference between the groups (P >0.05). CONCLUSION: It may be concluded that amoxicillin/metronidazole improves clinical and microbiologic results of FMUD in GAgP treatment.


Asunto(s)
Periodontitis Agresiva/terapia , Amoxicilina/uso terapéutico , Antibacterianos/uso terapéutico , Antiinfecciosos/uso terapéutico , Metronidazol/uso terapéutico , Desbridamiento Periodontal/métodos , Terapia por Ultrasonido/métodos , Adulto , Aggregatibacter actinomycetemcomitans/efectos de los fármacos , Periodontitis Agresiva/microbiología , Amoxicilina/administración & dosificación , Antibacterianos/administración & dosificación , Antiinfecciosos/administración & dosificación , Carga Bacteriana/efectos de los fármacos , Bacteroides/efectos de los fármacos , Índice de Placa Dental , Combinación de Medicamentos , Femenino , Estudios de Seguimiento , Líquido del Surco Gingival/inmunología , Hemorragia Gingival/terapia , Recesión Gingival/terapia , Humanos , Interleucina-10/análisis , Interleucina-1beta/análisis , Masculino , Metronidazol/administración & dosificación , Pérdida de la Inserción Periodontal/terapia , Índice Periodontal , Bolsa Periodontal/terapia , Placebos , Porphyromonas gingivalis/efectos de los fármacos , Método Simple Ciego , Resultado del Tratamiento
17.
Artículo en Inglés | MEDLINE | ID: mdl-22232720

RESUMEN

BACKGROUND: The aim of this study was to assess subgingival microbiological changes in smokers versus non-smokers presenting severe chronic periodontitis after supragingival periodontal therapy (ST). METHODS: Non-smokers (n=10) and smokers (n=10) presenting at least nine teeth with probing pocket depth (PPD) (≥5 mm), bleeding on probing (BoP), and no history of periodontal treatment in the last 6 months were selected. Clinical parameters assessed were plaque index (PI), BoP, PPD, relative gingival margin position (rGMP) and relative clinical attachment level (rCAL). Subgingival biofilm was collected before and 21 days after ST. DNA was extracted and the 16S rRNA gene was amplified with the universal primer pair, 27F and 1492R. Amplified genes were cloned, sequenced, and identified by comparison with known 16S rRNA sequences. Statistical analysis was performed by Student's t and Chi-Square tests (α=5%). RESULTS: Clinically, ST promoted a significant reduction in PI and PPD, and gain of rCAL for both groups, with no significant intergroup difference. Microbiologically, at baseline, data analysis demonstrated that smokers harbored a higher proportion of Porphyromonas endodontalis, Bacteroidetes sp., Fusobacterium sp. and Tannerella forsythia and a lower number of cultivated phylotypes (p<0.05). Furthermore, non-smokers featured significant reductions in key phylotypes associated with periodontitis, whereas smokers presented more modest changes. CONCLUSION: Within the limits of the present study, ST promoted comparable clinical improvements in smokers and non-smokers with severe chronic periodontitis. However, in smokers, ST only slightly affected the subgingival biofilm biodiversity, as compared with non-smokers.

18.
Med Mycol ; 50(5): 467-75, 2012 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-22114891

RESUMEN

Candida spp. are commensal microorganisms that are part of the microflora of different sites within the oral cavity. In healthy subjects, who have an unaltered immunological status, these yeasts do not cause disease. However, in immunosuppressed individuals whose condition may have been caused by diabetes mellitus, Candida spp. can express different virulence factors and may consequently become pathogenic. Studies have detected the presence of Candida spp. in periodontal sites of patients with chronic periodontitis, especially those that are immunologically compromised. However, the role of these microorganisms in the pathogenesis of periodontal disease is still unknown. The objectives of this study were: (1) to isolate and identify Candida albicans strains from subgingival sites of diabetic patients with chronic periodontitis; (2) to evaluate the following virulence factors; colony morphology, proteinase, phospholipase and hemolysin activities and cell surface hydrophobicity (CSH) under different atmospheric conditions; and (3) to determine the genetic patterns of these C. albicans isolates. Microbial samples were collected from subgingival sites and seeded on CHROMagar for subsequent identification of C. albicans by polymerase chain reaction (PCR). For the phenotypic tests, all strains of C. albicans were grown under reduced oxygen (RO) and anaerobiosis (ANA) conditions. Genotypes were defined by the identification through PCR of the transposable introns in the 25S rDNA. The results obtained relative to virulence factors were analyzed according to the atmospheric condition or genetic group, using Chi-square and Wilcoxon non-parametric tests. In this study, 128 strains were identified as C. albicans and of these, 51.6% were genotype B, 48.4% were genotype A and Genotype C was not found. Most of the strains were alpha-hemolytic in both atmospheric conditions, without a statistical difference. However, when comparing the genotypes, 46.1% of the genotype A strains were beta-hemolytic. In relation to colony morphology, 100% of the strains under ANA showed rough colonies, which were especially prevalent in genotype A isolates. In contrast, most of the colonies were smooth under RO. C. albicans strains did not produce proteinase and phospholipase activity in the total absence of oxygen. In RO, most strains had high proteinase activity and were positive by phospholipase tests (P < 0.05). Hydrophobicity was higher in anaerobiosis and was noted mainly for genotype A isolates. In conclusion, environmental oxygen concentration influenced the virulence factors of C. albicans strains isolated from subgingival sites of diabetic and periodontal patients. In addition, genotype A seems to be more virulent based on the phenotypic tests evaluated in this study.


Asunto(s)
Biopelículas/crecimiento & desarrollo , Candida albicans/clasificación , Candida albicans/genética , Candidiasis Bucal , Periodontitis Crónica , Diabetes Mellitus Tipo 2 , Encía/microbiología , Adulto , Candida albicans/aislamiento & purificación , Candida albicans/patogenicidad , Candidiasis Bucal/complicaciones , Candidiasis Bucal/microbiología , Periodontitis Crónica/complicaciones , Periodontitis Crónica/microbiología , Diabetes Mellitus Tipo 2/complicaciones , Diabetes Mellitus Tipo 2/microbiología , Femenino , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Fenotipo , Reacción en Cadena de la Polimerasa , Factores de Virulencia/genética
19.
Med Mycol ; 50(1): 43-9, 2012 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-21671830

RESUMEN

The objectives of this study were to evaluate clinical isolates of Candida albicans, particularly their adhesion to and invasion of gingival human fibroblasts in culture and to measure nitric oxide concentration (NO) produced by fibroblasts in the presence of these yeasts. Sixteen strains of C. albicans isolated from patients with chronic periodontitis and diabetes mellitus type II were divided on the basis of phenotypic tests into two groups, i.e., highly or weakly hydrophobic. Primary cultures of human fibroblasts were isolated from gingival biopsies and after subsequent subcultures, the cells were seeded into culture plates and incubated for 24 h. C. albicans strains were inoculated into these plates and maintained for 2 and 4 h to assess their adhesion and invasion, respectively. The number of adherent or invasive yeasts was evaluated by assessing colony-forming units (CFU). The production of NO by fibroblasts was also quantified. The results showed that strains with high hydrophobicity had a greater ability to adhere and invade fibroblasts (p < 0.05, ANOVA and Tukey). The production of NO was higher for the most hydrophobic strains, but did not reach statistical difference with the weakly hydrophobic isolates. These data indicated that the hydrophobicity may play a role in the adhesion and invasion of C. albicans in fibroblast cultures.


Asunto(s)
Candida albicans/patogenicidad , Adhesión Celular , Periodontitis Crónica/microbiología , Complicaciones de la Diabetes/microbiología , Diabetes Mellitus Tipo 2/microbiología , Fibroblastos/microbiología , Bolsa Periodontal/microbiología , Adulto , Anciano , Biopsia , Células Cultivadas , Recuento de Colonia Microbiana , Diabetes Mellitus Tipo 2/complicaciones , Femenino , Encía/citología , Humanos , Masculino , Persona de Mediana Edad , Óxido Nítrico/metabolismo
20.
Braz J Microbiol ; 43(3): 931-7, 2012 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-24031909

RESUMEN

The aim of the present study was to evaluate the frequency of detection of Mogibacterium timidum in subgingival samples of subjects with generalized aggressive periodontitis (GAgP) and uncontrolled diabetic and non-diabetic subjects with generalized chronic periodontitis (GChP). 48 patients with GAgP, 50 non-diabetic and 39 uncontrolled (glycated hemoglobin >7%) type 2 diabetic subjects with GChP were enrolled in this study. Subgingival biofilm were collected from deep pockets (probing depth > 7 mm). After DNA extraction, M. timidum was detected by Nested Polymerase Chain Reaction and chi-square test was used to data analysis (p>0.05). There were no differences in the frequency of detection of M. timidum between subjects with GAgP (35%) and non-diabetic subjects with GChP (40%) (p>0.05). The frequency of detection of M. timidum was significantly higher in deep pockets of diabetic subjects with GChP (56%) when compared to GAgP (p<0.05), but similar to non-diabetic subjects with GChP (p>0.05). The frequency of detection of M. timidum was higher in subjects GChP presenting uncontrolled type 2 diabetes mellitus, when compared to GAgP subjects.

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