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1.
Arch Microbiol ; 2021 Jan 23.
Artículo en Inglés | MEDLINE | ID: mdl-33484273

RESUMEN

An actinomycete strain, designated YIM 98757T, was isolated from the hypersaline sediment of Aiding Lake in Xinjiang province, north-west China. The strain grew well on most media tested and no diffusible pigment was produced. The substrate mycelium was well developed and fragmented. No spores were formed. The whole-cell hydrolysates contained meso-diaminopimelic acid as the cell-wall diamino acid. Xylose, galactose, ribose were the major whole-cell sugars. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and an unknown phospholipid. The predominant menaquinone was MK-8(H4). The major fatty acid was iso-C16:0. The DNA G + C content was 69.1 mol%. Phylogenetic analysis indicated that the isolate belongs to the genus Haloechinothrix. However, it differed from its closest relative, H. alba YIM 98757 T in many phenotypic and chemotaxonomic characteristics. Moreover, the DNA-DNA and ANI relatedness values between the novel isolate and H. alba YIM 93221 T were 53.3% and 92.5%, respectively. Based on comparative analysis of polyphasic taxonomic data, strain YIM 98757 T represents a novel species of the genus Haloechinothrix, for which the name Haloechinothrix aidingensis sp. nov. is proposed. The type strain is YIM 98757T (= CGMCC 4.7627T = CCTCC AA 2020012).

2.
J Int Med Res ; 49(1): 300060520984932, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-33461383

RESUMEN

OBJECTIVE: This study analyzed drug resistance and mutations profiles in Mycobacterium tuberculosis isolates in a surveillance site in Huairou District, Beijing, China. METHODS: The proportion method was used to assess drug resistance profiles for four first-line and seven second-line anti-tuberculosis (TB) drugs. Molecular line probe assays were used for the rapid detection of resistance to rifampicin (RIF) and isoniazid (INH). RESULTS: Among 235 strains of M. tuberculosis, 79 (33.6%) isolates were resistant to one or more drugs. The isolates included 18 monoresistant (7.7%), 19 polyresistant (8.1%), 28 RIF-resistant (11.9%), 24 multidrug-resistant (MDR) (10.2%), 7 pre-extensively drug-resistant (XDR, 3.0%), and 2 XDR strains (0.9%). A higher rate of MDR-TB was detected among previously treated patients than among patients with newly diagnosed TB (34.5% vs. 6.8%). The majority (62.5%) of RIF-resistant isolates exhibited a mutation at S531L in the DNA-dependent RNA polymerase gene. Meanwhile, 62.9% of INH-resistant isolates carried a mutation at S315T1 in the katG gene. CONCLUSION: Our results confirmed the high rate of drug-resistant TB, especially MDR-TB, in Huairou District, Beijing, China. Therefore, detailed drug testing is crucial in the evaluation of MDR-TB treatment.

3.
Biometals ; 2020 Oct 08.
Artículo en Inglés | MEDLINE | ID: mdl-33033991

RESUMEN

This study aims to explore the protective effects of quercetin against cadmium-induced nephrotoxicity utilizing metabolomics methods. Male Sprague-Dawley rats were randomly assigned to six groups: control, different dosages of quercetin (10 and 50 mg/kg·bw, respectively), CdCl2 (4.89 mg/kg·bw) and different dosages quercetin plus CdCl2 groups. After 12 weeks, the kidneys were collected for metabolomics analysis and histopathology examination. In total, 11 metabolites were confirmed, the intensities of which significantly changed (up-regulated or down-regulated) compared with the control group (p < 0.00067). These metabolites include xanthosine, uric acid (UA), guanidinosuccinic acid (GSA), hypoxanthine (Hyp), 12-hydroxyeicosatetraenoic acid (tetranor 12-HETE), taurocholic acid (TCA), hydroxyphenylacetylglycine (HPAG), deoxyinosine (DI), ATP, formiminoglutamic acid (FIGLU) and arachidonic acid (AA). When high-dose quercetin and cadmium were given to rats concurrently, the intensities of above metabolites significantly restored (p < 0.0033 or p < 0.00067). The results showed quercetin attenuated Cd-induced nephrotoxicity by regulating the metabolism of lipids, amino acids, and purine, inhibiting oxidative stress, and protecting kidney functions.

4.
PLoS One ; 15(7): e0236006, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32649724

RESUMEN

Halophiles are relatively unexplored as potential sources of novel species. However, little is known about the culturable bacterial diversity thrive in hypersaline lakes. In this work, a total of 343 bacteria from sediment samples of Aiding Lake, China, were isolated using nine different media supplemented with 5% or 15% (w/v) NaCl. The number of species and genera of bacteria recovered from the different media varied, indicating the need to optimize the isolation conditions. The results showed an unexpected level of bacterial diversity, with four phyla (Actinobacteria, Firmicutes, Proteobacteria, and Rhodothermaeota), fourteen orders (Actinopolysporales, Alteromonadales, Bacillales, Balneolales, Chromatiales, Glycomycetales, Jiangellales, Micrococcales, Micromonosporales, Oceanospirillales, Pseudonocardiales, Rhizobiales, Streptomycetales, and Streptosporangiales), including 17 families, 43 genera (including two novel genera), and 71 species (including four novel species). The predominant phyla included Actinobacteria and Firmicutes and the predominant genera included Actinopolyspora, Gracilibacillus, Halomonas, Nocardiopsis, and Streptomyces. To our knowledge, this is the first time that members of phylum Rhodothermaeota were identified in sediment samples from a salt lake.


Asunto(s)
Bacterias/aislamiento & purificación , Sedimentos Geológicos/microbiología , Bacterias/clasificación , Bacterias/efectos de los fármacos , Bacterias/genética , Biodiversidad , China , Lagos , Filogenia , ARN Ribosómico 16S/clasificación , ARN Ribosómico 16S/genética , Cloruro de Sodio/farmacología
5.
Environ Sci Pollut Res Int ; 27(21): 26484-26494, 2020 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-32367239

RESUMEN

A better understanding of farmers' perceptions of and responses to climate change is important for decision-makers to design more effective adaptation policies. This study investigates farmers' perceptions of climate change, actual adaption responses at the farm level, and factors influencing farmers' decisions on climate change adaptation in Wushen Banner, China. A questionnaire survey was conducted among 220 farmers with a random sampling technique. We found that farmers were generally concerned about climate change. Most farmers have adopted adaption measures to address the adverse effects of climate change. Adjusting farming behavior and using financial means were the main adaptation measures used by local farmers. The results revealed that the implementation of adaptation measures was constrained by the lack of technology, shortage of money, and poor infrastructure. The binary logistic regression results showed that farmers' socioeconomic characteristics, such as education, farming experience, and gender, had significant impacts on farmers' decisions to choose adaptation strategies. The regression results also indicated that farmers who believed climate change would affect their health were more willing to choose financial instruments, and farmers who believed climate change would affect their agricultural productions were likely to diversify their livelihoods. The findings provide some critical insights based on local perceptions of climate change and enhance our understanding of cognitive beliefs attached to adaptive responses.


Asunto(s)
Cambio Climático , Agricultores , Agricultura , China , Granjas , Humanos
6.
Ecotoxicol Environ Saf ; 199: 110755, 2020 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-32446099

RESUMEN

To increase the degree of immobilization of heavy metals subjected to sludge pyrolysis, we investigated the effects of pretreating sludge with Ochrobactrum supplementation on the immobilization of chromium (Cr) and copper (Cu) during sludge pyrolysis. The sequential extraction procedure was used to test the metallic forms of Cr and Cu. The immobilization of Cr and Cu was characterized with X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, etc. Results show that: 1) the addition of Ochrobactrum (1-8%) can accelerate the mineralization process in blank sludge and can accelerate the conversion of the oxidizable forms of Cr and Cu into the residual forms subjected to pyrolysis; 2) pretreatment with Ochrobactrum supplementation can inhibit the volatilization of Cr and Cu during sludge pyrolysis, particularly in the case of a high concentration of Cu. Notably, the pretreatment with Ochrobactrum can reduce 20.38-85.09% of the potential ecological risk of Cr and Cu. The pretreatment with Ochrobactrum contributes to the immobilization of Cr and Cu subjected to sludge pyrolysis and thus can prevent pollution of the environment. The results of this study can be used for harmless disposal of municipal sludge.


Asunto(s)
Cromo/análisis , Cobre/análisis , Ochrobactrum/química , Pirólisis , Aguas del Alcantarillado , Eliminación de Residuos Líquidos/métodos , Contaminantes Químicos del Agua/análisis , Adsorción , China , Microscopía Electrónica de Rastreo , Ochrobactrum/metabolismo , Oxidación-Reducción , Aguas del Alcantarillado/química , Aguas del Alcantarillado/microbiología
7.
Phys Rev Lett ; 124(12): 126601, 2020 Mar 27.
Artículo en Inglés | MEDLINE | ID: mdl-32281842

RESUMEN

Despite extensive experimental and theoretical efforts, the important issue of the effects of surface magnetic impurities on the topological surface state of a topological insulator (TI) remains unresolved. We elucidate the effects of Cr impurities on epitaxial thin films of (Bi_{0.5}Sb_{0.5})_{2}Te_{3}: Cr adatoms are incrementally deposited onto the TI held in ultrahigh vacuum at low temperatures, and in situ magnetoconductivity and Hall effect measurements are performed at each increment with electrostatic gating. In the experimentally identified surface transport regime, the measured minimum electron density shows a nonmonotonic evolution with the Cr density (n_{Cr}): it first increases and then decreases with n_{Cr}. This unusual behavior is ascribed to the dual roles of the Cr as ionized impurities and electron donors, having competing effects of enhancing and decreasing the electronic inhomogeneities in the surface state at low and high n_{Cr}, respectively. The magnetoconductivity is obtained for different n_{Cr} on one and the same sample, which yields clear evidence that the weak antilocalization effect persists and the surface state remains gapless up to the highest n_{Cr}, contrary to the expectation that the deposited Cr should break the time-reversal symmetry and induce a gap opening at the Dirac point.

8.
J Biochem Mol Toxicol ; 34(4): e22448, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-31967702

RESUMEN

This study aimed to investigate the protective effect of quercetin against the toxicity induced by chronic exposure to low levels of cadmium in rats by an ultra performance liquid chromatography mass spectrometer. Rats were randomly divided into six groups as follows: control group (C), low dose of quercetin group (Q1: 10 mg/kg·bw), high dose of quercetin group (Q2: 50 mg/kg·bw), cadmium chloride group (D), low dose of quercetin plus cadmium chloride group (DQ1), and high dose of quercetin plus cadmium chloride group (DQ2). Cadmium chloride (CdCl2 ) was administered to rats by drinking water ad libitum in a concentration of 40 mg/L. The final amount of CdCl2 ingested was estimated from the water consumption data to be 4.85, 4.91, and 4.89 mg/kg·bw/day, for D, DQ1, and DQ2 groups, respectively. After a 12-week treatment, the serum samples of rats were collected for metabonomics analysis. Ten potential biomarkers were identified for which intensities were significantly increased or reduced as a result of the treatment. These metabolites included isorhamnetin 4'-O-glucuronide, 3-indolepropionic acid, tetracosahexaenoic acid, lysophosphatidylcholine (LysoPC) (20:5), lysoPC (18:3), lysophosphatidylethanolamine (LysoPE) (20:5/0:0), bicyclo-prostaglandin E2, sulpholithocholylglycine, lithocholyltaurine, and glycocholic acid. Results indicated that quercetin exerted a protective effect against cadmium-induced toxicity by regulating lipid and amino acid metabolism, enhancing the antioxidant defense system and protecting liver and kidney function.

9.
Int J Syst Evol Microbiol ; 70(3): 1678-1683, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-31909706

RESUMEN

A novel bacterium, XHU 5135T, belonging to the genus Aidingimonas, was isolated from a salt lake sample collected in Xinjiang Province, north-west PR China. The isolate was Gram-stain-negative, rod-shaped and non-motile. The strain was catalase-positive and oxidase-negative. Growth occurred at NaCl concentrations of 5-25 % (optimum, 10-13 %), at 13-41 °C (35-37 °C) and at pH 6.0-10.0 (pH 7.0-8.0). The predominant ubiquinone was Q-9. The major fatty acids were C19 : 0 cyclo ω8c and C16 : 0. The G+C content of the genomic DNA was 58.1 mol%. The affiliation of strain XHU 5135T with the genus Aidingimonas was confirmed by 16S rRNA gene sequence comparisons. The closest type strain was Aidingimonas halophile YIM 90637T, which showed a 16S rRNA gene sequence similarity of 97.5 %. The ANI value between XHU 5135T and the closest type strain was 80.01 %. The estimated digital DNA-DNA hybridization estimate value between strain XHU 5135T and the closest type strain was 22.80 %. Phenotypically, the characteristics of XHU 5135T were shown to differ from the most closely related species, A. halophila. On the basis of the data from this polyphasic study, strain XHU 5135T represents a novel species of the genus Aidingimonas, for which the name Aidingimonas lacisalsi sp. nov. is proposed. The type strain is strain XHU 5135T (=CCTCC AB 2016344T=KCTC 42945T=DSM 104700T).


Asunto(s)
Halomonadaceae/clasificación , Lagos/microbiología , Filogenia , Salinidad , Técnicas de Tipificación Bacteriana , Composición de Base , China , ADN Bacteriano/genética , Ácidos Grasos/química , Halomonadaceae/aislamiento & purificación , Hibridación de Ácido Nucleico , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Ubiquinona/química
10.
Environ Technol ; 41(5): 658-668, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-30074861

RESUMEN

Monolayers of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane have been established on magnetite nanoparticles to develop a novel magnetic adsorbent for fast decontamination of hexavalent chromium (Cr(VI)) from water. Results indicated that monolayer adsorption of the silane from water took place at low concentrations (<300 mg/L) and around 100% surface coverage was obtained at temperatures ≥90°C. The hydrolysed silane was anchored to the magnetite surface through condensation reactions between its silanol groups and the surface hydroxyl groups of magnetite. The functional amine groups were protonated by acid treatment for adsorbing Cr(VI). The monolayer of the silane on magnetite (MSM) with approximately 100% surface coverage showed extremely rapid adsorption kinetics for Cr(VI), such that the process was complete within 1 min. This enables the treatment of large amounts of sewage per unit time. The adsorption capacity for Cr(VI) was 8.0 mg/g, as estimated from the Langmuir isotherm model. The saturation magnetization of the MSM reached 64.16 emu/g, allowing easy magnetic recovery from water. In the presence of up to 50-fold molar excesses of chloride and nitrate anions, little effect on Cr(VI) removal was seen, but moderate and large impacts were observed with sulphate and hydroxyl anions, respectively. Desorption of adsorbed Cr(VI) and regeneration of the MSM were successfully achieved by NaOH and HCl treatments to deprotonate and protonate the amine groups, respectively. By selecting a silane with suitable functional groups, the surface properties may be tailored for a particular pollutant.


Asunto(s)
Nanopartículas de Magnetita , Compuestos de Organosilicio , Contaminantes Químicos del Agua , Purificación del Agua , Adsorción , Cromo , Concentración de Iones de Hidrógeno , Cinética , Agua
11.
Environ Int ; 134: 105322, 2020 01.
Artículo en Inglés | MEDLINE | ID: mdl-31739135

RESUMEN

The separation of urine at source for phosphorus (P) recovery is attractive taking into account the high P concentration and small volume. However, the treatment of urine is still challenging due to its unpleasant odor and hygiene problems. Because the above problems could be solved by acidification to keep the pH of urine below 4, we propose a novel strategy to recover P from acidified urine using tailored hydrous zirconia-coated magnetite nanoparticles (Fe3O4@ZrO2). This strategy involves the selective adsorption of phosphate by easily separable and reusable Fe3O4@ZrO2, the desorption of adsorbed phosphate, and the precipitation of desorbed phosphate as calcium phosphate fertilizer. The results indicated that at pH 4, the P in synthetic urine was selectively adsorbed and could be exhausted using Fe3O4@ZrO2. Nearly all (>97.5%) of the sequestered P on the Fe3O4@ZrO2 nanoparticles was stripped using ≥1 M NaOH solution and ~100% of the stripped P was then successfully transformed into calcium phosphate, upon adding CaCl2 at pH >12 and a Ca/P molar ratio of 3. The liquid/solid (Fe3O4@ZrO2 particles) mixture could be conveniently separated for reuse using an external magnetic field. The reusability of the Fe3O4@ZrO2 nanoparticles in the extraction of P from synthetic urine was confirmed using five cycles of the adsorption-desorption process and their performance validated using real urine samples. The mechanism of phosphate adsorption was investigated using XPS, FTIR and zeta potential measurements, showing that phosphate was chemically adsorbed on the surface through direct coordination to zirconium atom via ligand exchange.


Asunto(s)
Nanopartículas de Magnetita , Fósforo/orina , Ácidos , Adsorción , Fraccionamiento Químico/métodos , Humanos , Concentración de Iones de Hidrógeno , Fosfatos/aislamiento & purificación
12.
Ecotoxicol Environ Saf ; 189: 109984, 2020 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-31767461

RESUMEN

In this study, we analyzed the effects of sludge aging pre-treatment on the stabilization mechanisms of heavy metals during sludge pyrolysis. First, the form of copper (Cu) and chromium (Cr) was conducted using the sequential extraction procedure proposed by the European Community Bureau of Reference (BCR). The stabilization mechanisms for the sludge pyrolysis of Cu and Cr were then analyzed using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). Results indicate the following: 1) with aging pre-treatment, the improvement in adsorption performance and the formation of newly crystallized materials, like polyhydroxy copper phosphate and chromium phosphate minerals, occur concurrently with the stabilization of heavy metals during pyrolysis; 2) after four weeks of aging, active functional groups like amino and carboxyl groups were significantly sharpened, and caused sustained complexation of the heavy metals. Results suggested that the aging pre-treatment aided the stabilization of heavy metals during sludge pyrolysis. Notably, the aging effect can decrease the potential ecological risk of heavy metals.


Asunto(s)
Metales Pesados/química , Pirólisis , Aguas del Alcantarillado/química , Eliminación de Residuos Líquidos/métodos , Contaminantes Químicos del Agua/química , Adsorción , Cromo/química , Cobre/química , Cristalización
13.
Artículo en Inglés | MEDLINE | ID: mdl-31877669

RESUMEN

Improving local farmers' climate change adaptive capacity is an important policy issue in rural China. This study investigates farmers' risk cognition, risk preferences and climate change adaptive behavior. Based on unique data from a survey and a paired lottery experiment completed by 240 rural farmers in Chongqing City of China, this paper finds that farmers have a pessimistic risk cognition towards climate change and the typical farmers are risk-averse and loss-averse. Risk cognition and adaptation cognition have significantly positive influences on climate change adaptive behavior, and loss aversion has a significantly positive influence on farmers' adaptation decisions. Loss aversion exerts a positive impact on risk cognition and adaptation cognition, and risk aversion has a positive impact on adaptation cognition. This paper contributes to the emerging literature that relates risk preference in experiments and risk cognition to farmers' climate change adaptive behavior.


Asunto(s)
Adaptación Psicológica , Cambio Climático , Agricultores , Modelos Teóricos , Aclimatación , Agricultura/estadística & datos numéricos , China , Cognición , Recolección de Datos , Toma de Decisiones , Humanos , Análisis de Clases Latentes , Políticas , Registros , Riesgo , Población Rural
14.
Eur J Clin Invest ; 49(12): e13179, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31610022

RESUMEN

BACKGROUND: Everolimus is an effective immunosuppressant in organ transplantation without impaired renal function. The present study aimed to evaluate the efficacy and safety of everolimus therapy in liver transplant recipients. MATERIALS AND METHODS: A systematic literature search was conducted to identify the eligible studies. The quality of the included studies was assessed. The outcomes of interest were biopsy-proven acute rejection (BPAR), graft loss, death, renal function and adverse events. RESULTS: Eight trials involving 1570 participants were included. Compared to the standard exposure to calcineurin inhibitors (CNIs), the incidences of BPAR, graft loss and death were not increased in the everolimus combined with reduced CNIs group. The renal function was significantly improved after everolimus combined with reduced CNI therapy, and the glomerular filtration rate (GFR) was estimated to be elevated by 5.59 (95% CI: 2.17-9.01, P = .001) as compared to the standard exposure to CNIs. The risk of any adverse event was increased by everolimus combined with reduced CNI therapy (RR = 1.22, 95% CI: 1.04-1.42, P = .01) as compared to the standard exposure to CNIs. The likelihood of infection was not associated with the regimen. Any publication bias was not identified. CONCLUSIONS: Although everolimus combined with reduced CNI therapy significantly improved the renal function in liver transplant recipients, it did not influence the incidence of BPAR, graft loss and death. This regimen might be associated with an increased risk of adverse events, which needs to be elucidated further.


Asunto(s)
Inhibidores de la Calcineurina/administración & dosificación , Everolimus/uso terapéutico , Rechazo de Injerto/prevención & control , Supervivencia de Injerto , Inmunosupresores/uso terapéutico , Trasplante de Hígado , Mortalidad , Quimioterapia Combinada , Humanos , Insuficiencia Renal/inducido químicamente
15.
Environ Sci Technol ; 53(21): 12449-12457, 2019 Nov 05.
Artículo en Inglés | MEDLINE | ID: mdl-31573182

RESUMEN

Phosphorus-inactivating agents (PIAs) have increasingly been applied and extensively investigated to control internal phosphorus loading in lakes. However, little is known about the behavior of PIA-amended sediment in terms of phosphorus immobilization and release when the sediment is resuspended in the photic layer, whose environment differs from the lake bed. Lanthanum-modified bentonite (LMB) is a popular PIA product. In this study, the 33 day core incubation experiment under dark conditions showed that capping sediment with LMB efficiently decreased the concentration of total phosphorus, total dissolved phosphorus, and dissolved inorganic phosphorus (DIP) by 90, 87, and 99%, respectively. Resuspension into overlying water under light conditions at high pH, high dissolved organic carbon, and in the presence of algae significantly impedes the performance of LMB. However, the adoption of a higher LMB dose improved the performance, including a reduction in the phosphorus level and control of algal growth. The dynamics of the phosphorus migration when the LMB-inactivated sediment was resuspended into the photic zone mainly involves the release of DIP from the sediment and the uptake of DIP by algae and LMB. In conclusion, a higher dose is needed in the PIA (particularly Phoslock) application in shallow productive lakes where sediment resuspension occurs frequently.


Asunto(s)
Lagos , Contaminantes Químicos del Agua , Bentonita , Eutrofización , Sedimentos Geológicos , Fósforo
16.
Environ Pollut ; 247: 9-17, 2019 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-30648618

RESUMEN

Release of phosphorus (P) from sediment to overlying water has to be dealt with to address algal blooms in eutrophic lakes. In this study, the sediment from the Lake Taihu was amended with lanthanum modified zeolite (LMZ) to reduce P release under different pH, temperature and anaerobic conditions. LMZ performed well, to decreasing P concentration in Lake Taihu water in the presence of sediment. The EPC0 value, the critical P concentration at which there was neither P adsorption nor P release, was lowered by adding LMZ, suggesting that amendment with LMZ could diminish the risk of P release from the sediment. From the Langmuir isotherm model, the adsorption capacity of phosphate by LMZ was estimated to be 64.1 mgP/g. The LMZ-amended sediment had a higher content of stable P forms (HCl-P and Res-P) and a lower content of P forms with a high (NH4Cl-P and BD-P) or medium-high (NaOH-P and Org-P) risk of release, when compared with the original sediment. The fractionation simulates conditions which release potentially mobile P which can then be simply re-bound to LMZ. At high pH (>9.0), anaerobic condition or high temperature promoted the liberation of P from sediment. However, P release could be greatly inhibited by LMZ. In addition, although Mn2+ and NH4+ ions were released from sediment under the anaerobic condition, the release could also be hindered by adding LMZ. LMZ is a promising P inactivation agent to manage eutrophication in the sediment of Lake Taihu.


Asunto(s)
Sedimentos Geológicos/química , Fósforo/análisis , Contaminantes Químicos del Agua/análisis , Zeolitas/química , Adsorción , China , Restauración y Remediación Ambiental , Eutrofización , Lagos/química , Lantano , Fosfatos , Fósforo/química , Contaminantes Químicos del Agua/química
17.
ACS Appl Mater Interfaces ; 10(40): 34283-34290, 2018 Oct 10.
Artículo en Inglés | MEDLINE | ID: mdl-30209939

RESUMEN

A porous silicon and carbon composite (PSi/C) with granadilla-like structure as an anode material for lithium-ion batteries has been easily fabricated by spray drying and subsequent pyrolysis treatments. For the PSi/C, yolk-shell-structured Si/C nanobeads are equably distributed inside the porous carbon framework. The key point of this work is the combination of the advantages of both the yolk-shell structure and porous structure in one system. The void space inside the yolk-shell Si/C nanobeads and the interconnected three-dimensional porous carbon frameworks can effectively enhance the cyclic stability and conductivity of this composite. As expected, PSi/C with 15.4% silicon content exhibited a specific capacity as high as 1357.43 mAh g-1 and retained 933.62 mAh g-1 beyond 100 cycles at 100 mA g-1. Moreover, it showed a reversible specific capacity as high as 610.38 mAh g-1 at 1000 mA g-1, even after 3000 cycles.

18.
Biochem Biophys Res Commun ; 505(2): 426-431, 2018 10 28.
Artículo en Inglés | MEDLINE | ID: mdl-30266402

RESUMEN

BACKGROUND: Postmenopausal osteoporosis (PMOP) is a metabolic bone disease caused by unbalance between osteoblast bone formation and osteoclast bone resorption. In this study, the moderating effect of DGCR5 on osteogenic differentiation and its role in PMOP was assessed. METHODS: The expression levels of DGCR5, miR-30d-5p, and Runt-related transcription factor 2 (Runx2) mRNA and protein were determined by qRT-PCR and western blot, separately. The bone marrow human mesenchymal stem cells (hMSCs) were isolated from bone marrow of patients with PMOP or the healthy control. ALP activity and bone mineral density (BMD) were detected to reflect the osteogenic differentiation status. RIP and RNA pull-down assay were performed to explore the combination and interaction between DGCR5 and miR-30d-5p. RESULTS: Compared with the healthy control group (n = 20), DGCR5 was down-regulated in hMSCs from patients with PMOP (n = 20). Overexpression of DGCR5 induced osteogenic differentiation of hMSCs. DGCR5 up-regulated the expression of Runx2 through miR-30d-5p. DGCR5 up-regulated the expression of Runx2 through miR-30d-5p to induce osteogenic differentiation of hMSCs. CONCLUSION: DGCR5 negatively regulates miR-30d-5p, and it up-regulates Runx2 through miR-30d-5p, thereby inducing osteogenic differentiation of hMSCs, which may help to delay PMOP development.


Asunto(s)
Subunidad alfa 1 del Factor de Unión al Sitio Principal/metabolismo , MicroARNs/metabolismo , Osteogénesis , ARN Largo no Codificante/metabolismo , Diferenciación Celular , Células Cultivadas , Humanos , Células Madre Mesenquimatosas/citología , MicroARNs/genética , Osteoblastos/citología , Osteoporosis Posmenopáusica/etiología , ARN Largo no Codificante/genética
19.
Arch Microbiol ; 199(9): 1237-1242, 2017 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-28577180

RESUMEN

A novel Gram-negative rod, endophytic bacterium, designated strain TMCC 8258T, was isolated from the root of Camellia sinensis collected from Puer, south-west China. Comparative 16S rRNA gene sequence analysis showed that the strain belongs to the family Sphingobacteriaceae and a neighbour-joining phylogenetic tree suggested that strain TMCC 8258T formed a cluster with the type strain of Olivibacter ginsengisoli (showed the highest 16S rRNA gene sequence similarity of 95.8%). Chemotaxonomic data [major fatty acid iso-C15:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), iso-C17:0 3-OH and major respiratory quinone MK-7] confirmed the affiliation of strain TMCC 8258T to the genus Olivibacter. The G + C content was 39.1 mol %. The results of the phylogenetic analysis, together with the physiological, morphological and biochemical tests, suggested that strain TMCC 8258T should be classified as representing a novel species of the genus Olivibacter, for which the name Olivibacter flavus is proposed. The type strain is TMCC 8258T (=CGMCC 1.16141 = KCTC 42683).


Asunto(s)
Bacteroidetes , Camellia sinensis/microbiología , ADN Bacteriano/genética , Raíces de Plantas/microbiología , Técnicas de Tipificación Bacteriana , Bacteroidetes/clasificación , Bacteroidetes/genética , Bacteroidetes/aislamiento & purificación , Composición de Base/genética , China , Ácidos Grasos/análisis , Filogenia , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN
20.
Arch Microbiol ; 199(9): 1231-1235, 2017 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-28573482

RESUMEN

A novel actinomycete strain, designated XHU 5301T, was isolated from a hypersaline habitat, China. The strain was aerobic, Gram-stain positive and the optimum NaCl concentration for growth was 7-9% (w/v). Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of strain XHU 5301T showed that the organism was most closely related to Glycomyces halotolerans TRM 40137T (96.0%). The whole-cell sugar pattern consisted of glucose and galactose. The predominant menaquinone was MK-10(H4), MK-10(H2), and MK-9(H4). The major fatty acids were anteiso-C15: 0, iso-C15: 0, iso-C16: 0, and methyl-C19: 0. The polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol, and one unknown phospholipid. The G+C content of the genomic DNA was 72.5 mol %. The novel species Glycomyces xinjiangensis sp. nov. was proposed, with strain XHU 5301T (=CCTCC AA 2016043T =KCTC 39689T) as the type strain of Glycomyces xinjiangensis.


Asunto(s)
Actinomycetales , ADN Bacteriano/genética , Actinomycetales/clasificación , Actinomycetales/genética , Actinomycetales/aislamiento & purificación , Técnicas de Tipificación Bacteriana , Composición de Base/genética , China , Ecosistema , Ácidos Grasos/análisis , Fosfolípidos/análisis , Filogenia , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Cloruro de Sodio/análisis , Vitamina K 2/análisis
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