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1.
J Oral Sci ; 62(4): 353-355, 2020 Sep 26.
Artículo en Inglés | MEDLINE | ID: mdl-32741852

RESUMEN

Computer-aided design/computer-aided manufacturing (CAD/CAM) restorative materials have been widely used owing to a number of advantages, including stable quality of the materials, lower costs, and time-saving factors. Resin-based CAD/CAM materials for definitive restorations are classified into two groups: dispersed nanoparticle-filled composite resin and polymer-infiltrated-ceramic-network materials. Resin-based CAD/CAM materials have been applied to single crown restorations as a monolithic structure for the posterior region. In addition, resin-based CAD/CAM restorations have been applied recently for the anterior area. This literature review summarizes clinical outcomes, such as survival rates and clinical complications of single crown restorations fabricated with resin-based CAD/CAM materials.


Asunto(s)
Diseño Asistido por Computadora , Coronas , Cerámica , Resinas Compuestas , Materiales Dentales , Porcelana Dental , Diseño de Prótesis Dental , Ensayo de Materiales
2.
Dent Mater J ; 39(5): 869-876, 2020 Sep 29.
Artículo en Inglés | MEDLINE | ID: mdl-32448848

RESUMEN

To investigate the effect of luting agent type on fracture loads of implant-supported ceramic prostheses in premolar region. Ninety-nine implant-abutment complexes were divided into three different implant-supported prostheses: monolithic yttria-partially stabilized zirconia (Y-PSZ) restorations (MPZ specimens), porcelain layered on yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) restorations (PLZ specimens), and monolithic lithium disilicate ceramic restorations (MLD specimens). Implant-supported prostheses were luted with adhesive resin luting agent (RLA), glass ionomer cement (GIC), or zinc phosphate cement (ZPC). For MPZ and MLD specimens, fracture loads were significantly higher for RLA group than for GIC and ZPC groups. For PLZ specimens, fracture loads did not significantly differ in relation to luting agent. Fracture loads were significantly higher for MPZ specimens than for other test specimens, regardless of luting agent. Use of an adhesive resin luting agent is recommended for placement of premolar implant-supported monolithic Y-PSZ and lithium disilicate ceramic prostheses.


Asunto(s)
Cementos Dentales , Implantes Dentales , Diente Premolar , Cerámica , Coronas , Porcelana Dental , Análisis del Estrés Dental , Ensayo de Materiales , Cementos de Resina , Circonio
3.
J Prosthodont Res ; 64(3): 313-318, 2020 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-31859081

RESUMEN

PURPOSE: The objective of the present study was to evaluate fracture loads of screw-retained implant-supported zirconia prostheses after artificial aging. METHODS: Four types of screw-retained implant-supported prostheses were fabricated (n=11 each); porcelain-veneered zirconia prosthesis (PVZ), indirect composite-veneered zirconia prosthesis (IVZ), porcelain-fused-to-metal prosthesis (PFM), and monolithic zirconia prosthesis (ML). The specimens were subjected to 10,000 thermocycles and cyclic loading for 1.2 million cycles. Fracture loads were measured, and the data were analyzed with the Kruskal-Wallis and Steel-Dwass tests (α=0.05). RESULTS: All specimens survived the artificial aging procedures. The fracture loads for the PVZ (1.52kN), IVZ (1.62kN), and PFM groups (1.53kN) did not significantly differ; however, the fracture load for the ML group (6.61kN) was significantly higher than those for the other groups. The fracture load for the IVZ group was comparable to those for the PVZ and PFM groups. CONCLUSIONS: The monolithic zirconia prostheses exhibited significantly higher fracture loads than the bilayered prostheses. All the investigated types of screw-retained implant-supported zirconia prostheses appear sufficient to resist posterior masticatory forces during long-term clinical use.


Asunto(s)
Prótesis Dental de Soporte Implantado , Circonio , Tornillos Óseos , Coronas , Porcelana Dental , Fracaso de la Restauración Dental , Análisis del Estrés Dental , Ensayo de Materiales , Estrés Mecánico
4.
Artículo en Inglés | MEDLINE | ID: mdl-31621439

RESUMEN

Purpose: To evaluate the usefulness of 2D-shear wave elastography (2D-SWE) in the prediction of type II endoleaks after endovascular aneurysm repair (EVAR) of abdominal aortic aneurysms (AAA). Material and methods: Twenty-nine patients underwent EVAR for AAA, and 2D-SWE was performed after EVAR. Follow-up contrast-enhanced CT and ultrasonography were performed to evaluate endoleaks in all patients. The median follow-up period was 12 months (range, 3-12 months). Patients were divided into two groups: one with an endoleak (endoleak group) and another without it (control group). We compared the elasticity index (EI) of intraluminal thrombus (ITL) and fresh thrombus (FT) between the two groups. Results: Type II endoleaks were confirmed in five of the 29 patients (endoleak group), and there were no endoleaks in the other 24 (control group). ILT was observed in 21 patients of the control group and in all patients of the endoleak group. There was a difference only in EI of ILT; the mean EI (± standard deviation) of ILT was 89 ± 16 kPA in the control group and 113 ± 25 kPA in the endoleak group (p=.037). Conclusions: High EI of ILT may predict the occurrence of type II endoleaks after EVAR of AAA.

5.
J Prosthodont Res ; 63(2): 140-144, 2019 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-30446411

RESUMEN

PURPOSE: To determine the effect of veneering material and framework design on fracture loads of implant-supported zirconia molar fixed dental prostheses (FDPs). METHODS: Sixty-six zirconia FDPs were manufactured onto two implants and classified as uniform thickness (UT) or anatomic design (AD). These framework design groups were then further divided into three subgroups (n=11): feldspathic porcelain-veneered zirconia FDPs (PVZ), indirect composite-veneered zirconia FDPs (IVZ), and metal-ceramic FDPs (MC). The FDPs were luted on the implant abutments and underwent fracture load testing. Significant differences were assessed by the Kruskal-Wallis test and Mann-Whitney U-test (α=0.05). RESULTS: For UT group, median fracture load was significantly higher for the IVZ (1.87kN) and MC (1.90kN) specimens than for the PVZ specimens (1.38kN) (p<0.05). In the AD group, the IVZ specimens had the highest median fracture load (4.10kN) of the three groups tested. The AD group exhibited higher median fracture loads than the UT group in all subgroups. CONCLUSIONS: Indirect composite appears to be a useful alternative to feldspathic porcelain as the layering material for implant-supported zirconia FDPs. The AD group had higher fracture loads than UT group. In addition, implant-supported indirect composite-veneered zirconia-based FDPs appear to be clinically feasible.


Asunto(s)
Implantes Dentales , Materiales Dentales , Diseño de Prótesis Dental , Prótesis Dental de Soporte Implantado , Análisis del Estrés Dental , Coronas con Frente Estético , Ensayo de Materiales , Diente Molar , Circonio , Diseño Asistido por Computadora , Porcelana Dental , Humanos , Estrés Mecánico
6.
Eur J Oral Sci ; 126(6): 507-511, 2018 12.
Artículo en Inglés | MEDLINE | ID: mdl-30289591

RESUMEN

This study investigated the effect of preparation design on the marginal and internal adaptation of laminate veneers (LVs) fabricated from translucent zirconia. Thirty-three resin teeth were prepared for LVs of three designs: window preparation (WP); incisal shoulder preparation (ISP); and incisal palatal chamfer preparation (IPP). Marginal adaptation was evaluated by measuring the vertical discrepancy between the LV margin and the finish line at 60 points. The internal adaptation was assessed by measuring the internal space width as the distance between the LV and the tooth at cervical, central, and incisal sites after sectioning. At the incisal, mesial, and distal sites, mean marginal discrepancies were significantly lower in the WP group than in the other two groups; the IPP group had the highest marginal discrepancies. At incisal sites, the median internal space was significantly higher in the IPP group than in the WP and ISP groups and higher in the ISP group than in the WP group. At the incisal site, marginal and internal adaptations were better for the non-overlap translucent zirconia LV design (WP) than for the overlap designs (ISP and IPP). The characteristics of the translucent zirconia LVs used in the present study indicate acceptable clinical performance.


Asunto(s)
Adaptación Marginal Dental , Diseño de Prótesis Dental/métodos , Coronas con Frente Estético , Preparación Protodóncica del Diente/métodos , Circonio/química , Cerámica , Diseño Asistido por Computadora , Humanos , Procesamiento de Imagen Asistido por Computador/métodos , Incisivo , Cementos de Resina , Propiedades de Superficie
7.
Clin Oral Implants Res ; 29(4): 396-403, 2018 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-29484710

RESUMEN

OBJECTIVES: The purpose of this in vitro study was to evaluate fracture loads of implant-supported zirconia-based prostheses fabricated with different veneer materials (resin-based material and lithium disilicate ceramics). MATERIAL AND METHODS: Forty-four zirconia-based molar prostheses were fabricated on dental implants and divided into four groups (n = 11): zirconia-based prostheses veneered with feldspathic porcelain (ZVF), zirconia-based prostheses bonded with the lithium disilicate glass-ceramic veneer (ZBD), zirconia-based prostheses veneered with indirect composite resin (ZVC), and zirconia-based prostheses bonded with composite materials fabricated from a CAD/CAM resin block (ZBC). The zirconia-based prostheses and abutments were adhesively bonded with a dual-polymerized resin-based luting material. Fracture load was determined using compression load to the prostheses with a universal testing machine. The data were analyzed with one-way analysis of variance (ANOVA) and Tukey's HSD test (α = .05). RESULTS: The mean fracture load was significantly higher in the ZBC group (3.95 kN) than in the ZVC group (3.28 kN). No significant difference in fracture load was found among the ZVF (3.52 kN), ZBD (3.48 kN), and ZVC groups. CONCLUSIONS: The adhesively bonded veneering technique enhances fracture resistance of implant-supported zirconia-based prostheses fabricated with a resin-based material. All implant-supported zirconia-based restorations tested should resist physiologic masticatory forces in the oral environment.


Asunto(s)
Prótesis Dental de Soporte Implantado , Análisis del Estrés Dental , Coronas con Frente Estético , Circonio , Diseño de Prótesis Dental , Ensayo de Materiales
8.
J Patient Saf ; 14(4): 227-233, 2018 12.
Artículo en Inglés | MEDLINE | ID: mdl-26076074

RESUMEN

OBJECTIVES: To explore the additional medical costs (AMCs) due to hospital-acquired falls (falls), as well as their impact on clinical services within hospitals under the nationally uniform universal health insurance system in Japan. METHODS: With the use of administrative profiling data based on accounting systems linked with the Japanese social insurance medical fee schedule, we analyzed data from 2 teaching hospitals: Shimane University Hospital (SUH) and St. Mary's Hospital (SMH). We extracted 588 fall cases from 4669 incident reports in SUH and 1168 fall cases from 7717 incident reports in SMH that potentially incurred AMCs. RESULTS: Additional medical costs were 364 ± 2129 USD for minor injuries and 4336 ± 3645 USD for major injuries at SUH (P < 0.001) and 114 ± 124 USD for minor injuries and 2267 ± 2811 USD for major injuries at SMH (P < 0.001). Among the clinical services provided, imaging services were the most frequently used, with 89.9% (n = 205) of 228 minor injuries at SUH and 86.7% (n = 339) of 391 minor injuries at SMH; imaging services were used in all major injury cases at both hospitals. Although the number of cases using additional procedure/surgery services was lower than those using imaging services at both hospitals, AMCs for procedure/surgery services accounted for the highest proportions of total AMCs in both hospitals. CONCLUSIONS: Although falls with minor injuries outnumbered falls with major injuries, fall-related AMCs for the latter were higher at both teaching hospitals because procedure/surgery services were required for cases with major injuries such as femoral neck and trochanteric fractures. The findings suggest that hospital administrators and policy makers have to take appropriate measures to prevent major injuries inpatients due to hospital-acquired falls.


Asunto(s)
Accidentes por Caídas/economía , Hospitales/estadística & datos numéricos , Medición de Riesgo/métodos , Femenino , Humanos , Masculino , Gestión de Riesgos
9.
Intern Med ; 56(15): 2085, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28768990
10.
Eur J Radiol ; 91: 116-123, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-28629557

RESUMEN

OBJECTIVE: To assess the value of contrast-enhanced MRI, apparent diffusion coefficient (ADC) measurement, and CA-125 measurement for differentiating borderline ovarian tumors (BOTs) from stage I malignant epithelial ovarian tumors (MEOTs). MATERIAL AND METHODS: This retrospective study included 43 patients with BOTs and 43 patients with stage I MEOTs who underwent contrast-enhanced MRI with DWI and CA-125 analysis. Two radiologists evaluated the MRI findings in consensus. Univariate and multivariate analyses were performed to detect the best predictor variables for MEOTs. RESULTS: Mixed cystic/solid and predominantly solid appearances, as well as thickened irregular septa, were more frequent in MEOTs. A papillary architecture and internal branching (PA&IB) pattern was more frequent in BOTs. MEOTs had thicker walls and septa, larger solid components, and higher CA-125 values. The mean ADC value of solid components (ADCmean) and minimum ADC value of whole lesions (ADCmin) were lower in MEOTs. Multivariate analysis revealed that ADCmin and maximum diameter of the solid components were independent indicators of MEOTs with an AUC, sensitivity, and specificity of 0.86, 81%, and 84%, respectively. CONCLUSION: ADCmin and maximum diameter of solid components were useful for differentiating BOTs from MEOTs.


Asunto(s)
Imagen por Resonancia Magnética/mortalidad , Neoplasias Ováricas/diagnóstico , Diagnóstico Diferencial , Femenino , Humanos , Modelos Logísticos , Neoplasias Ováricas/patología , Estudios Retrospectivos , Sensibilidad y Especificidad
11.
Clin Oral Implants Res ; 28(9): 1119-1126, 2017 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-27412687

RESUMEN

OBJECTIVES: The objective of this in vitro study was to investigate fracture loads of screw-retained zirconia-based molar restorations (hybrid abutment crown) fabricated with different restorative materials and designs. MATERIAL AND METHODS: Forty-four screw-retained zirconia-based molar restorations were fabricated on dental implants and divided into four groups (n = 11): porcelain-layered zirconia-based restorations (PLZ), indirect composite-layered zirconia-based restorations (ILZ), metal-ceramic restorations (MC), and monolithic zirconia restorations (MONO). The zirconia-based restorations in the PLZ, ILZ, and MONO groups were adhesively bonded on implant abutments with a dual-polymerized resin material. All restorations were tightened on implant bodies with titanium screws and were tested for fracture resistance. The Kruskal-Wallis test and Steel-Dwass test were used to evaluate differences in fracture loads (α = 0.05). RESULTS: As compared with the other groups, the MONO specimens had a significantly higher mean fracture resistance (7.54 kN); no significant differences were found among the PLZ (1.96 kN), ILZ (1.80 kN), and MC (1.45 kN) groups (P > 0.05). For the PLZ, ILZ, and MC groups, all specimens fractured within the layering materials. In contrast, the fracture mode for the MONO group was complete fracture of the restorations. CONCLUSIONS: All restorations withstood the masticatory forces. Fracture loads were significantly higher for screw-retained implant-supported monolithic zirconia restorations than for screw-retained bilayered restorations. For the screw-retained bilayered zirconia-based restorations, the fracture resistance of ILZ restorations was comparable to that of PLZ restorations and MC restorations.


Asunto(s)
Tornillos Óseos , Materiales Dentales , Prótesis Dental de Soporte Implantado , Análisis del Estrés Dental , Diente Molar , Circonio , Diseño de Implante Dental-Pilar , Ensayo de Materiales
12.
Kansenshogaku Zasshi ; Suppl 13: 8-14, 2015 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-26529982

RESUMEN

Limited use of linezolid for treating methicillin-resistant Staphylococcus aureus (MRSA) infection was approved in Japan in 2006. We report here the status of linezolid-resistant MRSAs in Japan. Eleven linezolid-resistant clinical isolates from 11 patients at six hospitals were collected from 2006 through 2008. The minimal inhibitory concentration (MIC) of linezolid in these strains varied from 8 to 64 µg/ml. All strains had at least one G2576T mutation in the chromosomal gene(s) encoding domain V of the 23S ribosomal RNA (rRNA). Chromosomal DNA encoding five copies of the domain V region was analyzed by polymerase chain reaction (PCR). Strains with the linezolid MICs of 64, 32, 16, and 8 µg/ml had the G2576T mutation(s) in four, three (or four), two, and one copy of the 23S rRNA genes, respectively. These results suggest that the level of linezolid resistance seems to be roughly correlated with the number of mutations in the genes encoding 23S rRNA. DNA samples from all 11 strains were subjected to pulsed-field gel electrophoresis and were classified into seven independent clones having >92% identity. Among the 11 patients, five had been treated with linezolid and the remainder, in two hospitals, had no history of prior linezolid use. The results suggested possible nosocomial infections by linezolid-resistant MRSA.


Asunto(s)
Antibacterianos/farmacología , Farmacorresistencia Bacteriana , Linezolid/farmacología , Infecciones Estafilocócicas , Staphylococcus aureus/aislamiento & purificación , ADN Bacteriano/genética , Genoma Bacteriano , Humanos , Japón , ARN Ribosómico 23S/genética , Infecciones Estafilocócicas/tratamiento farmacológico , Staphylococcus aureus/genética , Factores de Tiempo
13.
J Adhes Dent ; 17(3): 273-81, 2015 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-26159129

RESUMEN

PURPOSE: This study evaluated the long-term shear bond strength between an indirect composite material and a zirconia framework coated with silica-based ceramics, taking the effect of different primers into account. MATERIALS AND METHODS: A total of 165 airborne-particle abraded zirconia disks were subjected to one of three pretreatments: no pretreatment (ZR-AB), airborne-particle abrasion of zirconia coated with feldspathic porcelain (ZR-PO-AB), and 9.5% hydrofluoric acid etching of zirconia coated with feldspathic porcelain (ZR-PO-HF). An indirect composite material (Estenia C&B) was then bonded to the zirconia disks after they were treated with one of the following primers: Clearfil Photo Bond (CPB), Clearfil Photo Bond with Clearfil Porcelain Bond Activator (CPB + Activator), Estenia Opaque Primer (EOP), Porcelain Liner M Liquid B (PLB), or no priming (CON, control group). Shear bond strength was tested after 100,000 thermocycles, and the data were analyzed using the Steel-Dwass U-test (α = 0.05). RESULTS: For ZR-PO-AB and ZR-PO-HF specimens, bond strength was highest in the CPB+Activator group (25.8 MPa and 22.4 MPa, respectively). Bond strengths were significantly lower for ZR-AB specimens in the CON and PLB groups and for ZR-PO-AB specimens in the CON, CPB, and EOP groups. CONCLUSION: Combined application of a hydrophobic phosphate monomer (MDP) and silane coupling agent enhanced the long-term bond strength of indirect composite material to a zirconia coated with silica-based ceramics.


Asunto(s)
Cerámica/química , Materiales Biocompatibles Revestidos/química , Resinas Compuestas/química , Recubrimiento Dental Adhesivo , Materiales Dentales/química , Dióxido de Silicio/química , Circonio/química , Grabado Ácido Dental/métodos , Silicatos de Aluminio/química , Cementos Dentales/química , Grabado Dental/métodos , Porcelana Dental/química , Humanos , Ácido Fluorhídrico/química , Ensayo de Materiales , Metacrilatos/química , Poliuretanos/química , Compuestos de Potasio/química , Distribución Aleatoria , Cementos de Resina/química , Resistencia al Corte , Silanos/química , Estrés Mecánico , Temperatura , Tionas/química , Factores de Tiempo
14.
Minim Invasive Ther Allied Technol ; 24(2): 119-22, 2015 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-25140703

RESUMEN

OBJECTIVE: Superselective transcatheter arterial embolization (TAE) is important for lower gastrointestinal (GI) bleeding. A new 1.9-Fr. no-taper microcatheter has recently become available and can be inserted into a 2.7-Fr. microcatheter. We assessed the applicability of this new triple co-axial (triaxial) system to TAE for lower GI bleeding. MATERIAL AND METHODS: Five patients with lower GI bleeding underwent TAE with the triaxial system. The approach was via the femoral artery with a 4-Fr. sheath in all cases. The 4-Fr. catheter and triaxial system were inserted into the artery in which extravasation had occurred. Coil embolization was performed with 0.010-inch coils. We evaluated technical success rate, clinical success rate and complications. RESULTS: All five cases of bleeding occurred at the ascending colon, and were caused by diverticulosis in four cases, and an injury to the artery during polypectomy in one case. The 1.9-Fr. no-taper microcatheter could be inserted into the site of extravasation, the vasa recta, in all procedures and TAE was performed successfully. The disappearance of extravasation was confirmed in all cases following TAE. No patients exhibited any signs of recurrent bleeding or complication. CONCLUSION: The triaxial system appears to be effective and useful in superselective TAE for lower GI bleeding.


Asunto(s)
Cateterismo/instrumentación , Embolización Terapéutica/métodos , Hemorragia Gastrointestinal/terapia , Adulto , Anciano , Anciano de 80 o más Años , Colon , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos
15.
J Infect Chemother ; 20(9): 527-34, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-25066429

RESUMEN

We conducted an antibiotic susceptibility survey of 830 blood-borne methicillin resistant Staphylococcus aureus collected from nationwide hospitals in Japan over a three-year period from January 2008 through May 2011. Antibiotic susceptibility was judged according to the criteria recommended by the Clinical Laboratory Standard Institute. Over 99% of the MRSA showed to be susceptible to teicoplanin, linezolid, sulfamethoxazole/trimethoprim and vancomycin, and over 97% of them were susceptible to daptomycin, arbekacin and rifampin. The majority of the MRSA strains showed resistant to minocycline, meropenem, imipenem, clindamycin, ciprofloxacin, cefoxitin, and oxacillin in the rates of 56.6, 72.9, 73.7, 78.7, 89.0, 99.5, and 99.9%, respectively. Among the MRSA strains, 72 showed reduced susceptibility to vancomycin, including 8 strains (0.96%) of vancomycin-intermediate S. aureus (VISA), 54 (6.51%) of heterogeneous vancomycin-intermediate S. aureus (hVISA), and 55 (5.63%) of ß-lactam antibiotics-induced vancomycin resistant S. aureus (BIVR). Unexpectedly, among the 54 hVISA and 55 BIVR, 45 isolates (83.3% and 81.8%, respectively) showed both hVISA and BIVR phenotypes. A new trend of vancomycin resistance found in this study was that VISA strains were still prevalent among the bacteremic specimens. The high rates of the hVISA/BIVR two-phenotypic vancomycin resistance, and the prevalence of VISA in the bloodborne MRSA call attention in the MRSA epidemiology in Japan.


Asunto(s)
Antibacterianos/uso terapéutico , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Staphylococcus aureus Resistente a Meticilina/aislamiento & purificación , Infecciones Estafilocócicas/microbiología , Humanos , Japón , Pruebas de Sensibilidad Microbiana/métodos , Infecciones Estafilocócicas/sangre , Infecciones Estafilocócicas/tratamiento farmacológico , Resistencia a la Vancomicina/fisiología , beta-Lactamas/uso terapéutico
16.
Dev Comp Immunol ; 41(1): 68-76, 2013 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-23583523

RESUMEN

Both cellular and humoral immune responses are crucial to induce potent anti-tumor immunity, but most of currently conducted peptide-based cancer vaccines paid attention to cellular responses alone, and none of them are yet approved as a therapeutic modality against cancer patients. We investigated humoral immune responses to CTL epitope peptides derived from tumor-associated antigens in healthy donors and patients with various diseases to facilitate better understanding of their distribution patterns and potential roles. Bead-based multiplex assay, ELISA, and Western blotting were used to measure immunoglobulins reactive to each of 31 different CTL epitope peptides. Importantly, the sums of anti-peptide IgG levels specific to 31 CTL epitope peptides were well correlated with better overall survival (OS) in patients with malignant diseases. Our results suggested that humoral immune responses to CTL epitope peptides were widely detectable in humans. Measurement of immunoglobulins specific to CTL epitope peptides may provide a new biomarker for OS of patients with malignant diseases, although it still remains to be determined whether the correlations between humoral immune responses to epitope peptides and OS are observed only for the CTL epitopes used, or also for other panels of peptides. Quantity of circulating IgG reactive to these peptides was also discussed.


Asunto(s)
Antígenos de Neoplasias/inmunología , Biomarcadores de Tumor/inmunología , Carcinoma Hepatocelular/inmunología , Epítopos de Linfocito T/inmunología , Inmunidad Humoral , Inmunoglobulina G/inmunología , Neoplasias Hepáticas/inmunología , Linfocitos T Citotóxicos/inmunología , Adolescente , Adulto , Anciano , Antígenos de Neoplasias/genética , Artritis Reumatoide/genética , Artritis Reumatoide/inmunología , Biomarcadores de Tumor/genética , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/mortalidad , Epítopos de Linfocito T/genética , Femenino , Glomerulonefritis por IGA/genética , Glomerulonefritis por IGA/inmunología , Humanos , Inmunoglobulina G/genética , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/mortalidad , Masculino , Persona de Mediana Edad , Péptidos/genética , Péptidos/inmunología , Análisis de Supervivencia , Linfocitos T Citotóxicos/patología
17.
Case Rep Vasc Med ; 2013: 781698, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24383038

RESUMEN

We report a 74-year-old man with rebleeding following stent grafting for the celiac artery bleeding which developed after extended pancreaticoduodenectomy for cancers of the bile duct and stomach. The site of rebleeding seemed to be different from the site of the first bleeding, so it was considered not an endoleak but a new bleeding. It was successfully treated by placement of another stent graft.

18.
J Med Microbiol ; 61(Pt 7): 998-1002, 2012 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-22493282

RESUMEN

The aim of this study was to evaluate Etest for detectability of linezolid-resistant meticillin-resistant Staphylococcus aureus (MRSA). The MIC of linezolid obtained by the Etest method in 18 linezolid-resistant strains of MRSA was compared with that obtained using standard agar and broth dilution methods according to Clinical and Laboratory Standards Institute guidelines. The mean linezolid MIC obtained by Etest in 18 linezolid-resistant strains of MRSA using Mueller-Hinton (MH) agar was 12.6-fold lower than that obtained by the agar dilution method, with the result that 78 % of the linezolid-resistant strains were incorrectly classified as linezolid-susceptible. The MIC of linezolid by Etest on brain-heart infusion (BHI) agar had a mean value 2.5-fold lower than that obtained by the agar dilution method, suggesting that replacing MH agar with BHI agar considerably improved the detectability of linezolid-resistant MRSA. Use of blood agar (MH agar supplemented with 5 % sheep blood) and 48 h of incubation resulted in 100 % agreement with the agar and broth dilution methods. Thus, this study revealed that the Etest on MH agar and BHI agar yielded false-negative results in a significant fraction of the linezolid-resistant MRSA. Hence, the use of blood agar and prolonged incubation is highly recommended for the accurate detection of linezolid-resistant MRSA using Etest.


Asunto(s)
Acetamidas/farmacología , Antibacterianos/farmacología , Medios de Cultivo/química , Farmacorresistencia Bacteriana , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Oxazolidinonas/farmacología , Humanos , Linezolid , Pruebas de Sensibilidad Microbiana/métodos , Sensibilidad y Especificidad
19.
Antimicrob Agents Chemother ; 55(5): 2466-8, 2011 May.
Artículo en Inglés | MEDLINE | ID: mdl-21357291

RESUMEN

Methicillin-resistant Staphylococcus aureus with a MIC of linezolid of 4 µg/ml, isolated from a patient who had undergone unsuccessful linezolid therapy, yielded linezolid-resistant mutants in blood agar at 48 h of incubation. The resistant clones showed a MIC of linezolid ranging from 8 to 64 µg/ml and accumulated the T2500A mutation(s) of the rRNA genes. Emergence of these resistant clones appears to be facilitated by a cryptic mutation or mutations associated with chloramphenicol resistance.


Asunto(s)
Acetamidas/farmacología , Antibacterianos/farmacología , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Oxazolidinonas/farmacología , Farmacorresistencia Bacteriana/genética , Genes de ARNr/genética , Linezolid , Staphylococcus aureus Resistente a Meticilina/genética , Pruebas de Sensibilidad Microbiana , Mutación
20.
J Infect Chemother ; 17(1): 45-51, 2011 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-20607345

RESUMEN

Limited use of linezolid for treating methicillin-resistant Staphylococcus aureus (MRSA) infection was approved in Japan in 2006. We report here the status of linezolid-resistant MRSAs in Japan. Eleven linezolid-resistant clinical isolates from 11 patients at six hospitals were collected from 2006 through 2008. The minimal inhibitory concentration (MIC) of linezolid in these strains varied from 8 to 64 µg/ml. All strains had at least one G2576T mutation in the chromosomal gene(s) encoding domain V of the 23S ribosomal RNA (rRNA). Chromosomal DNA encoding five copies of the domain V region was analyzed by polymerase chain reaction (PCR). Strains with the linezolid MICs of 64, 32, 16, and 8 µg/ml had the G2576T mutation(s) in four, three (or four), two, and one copy of the 23S rRNA genes, respectively. These results suggest that the level of linezolid resistance seems to be roughly correlated with the number of mutations in the genes encoding 23S rRNA. DNA samples from all 11 strains were subjected to pulsed-field gel electrophoresis and were classified into seven independent clones having >92% identity. Among the 11 patients, five had been treated with linezolid and the remainder, in two hospitals, had no history of prior linezolid use. The results suggested possible nosocomial infections by linezolid-resistant MRSA.


Asunto(s)
Acetamidas/farmacología , Antibacterianos/farmacología , Staphylococcus aureus Resistente a Meticilina/efectos de los fármacos , Oxazolidinonas/farmacología , Infecciones Estafilocócicas/microbiología , Acetamidas/uso terapéutico , Antibacterianos/uso terapéutico , Infección Hospitalaria , ADN Bacteriano/genética , Farmacorresistencia Bacteriana Múltiple , Electroforesis en Gel de Campo Pulsado , Hospitales , Humanos , Japón , Linezolid , Staphylococcus aureus Resistente a Meticilina/genética , Pruebas de Sensibilidad Microbiana , Oxazolidinonas/uso terapéutico , Reacción en Cadena de la Polimerasa , ARN Ribosómico 23S/genética , Infecciones Estafilocócicas/tratamiento farmacológico
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