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1.
Artículo en Inglés | MEDLINE | ID: mdl-34389436

RESUMEN

BACKGROUND: The latest studies have considered the time-dependent structures in dynamic brain networks. However, the effect of periphery structures on the temporal flow of information remains unexplored in patients with major depressive disorder (MDD). In this work, we aimed to explore the pattern of interactions between brain regions in MDD across space and time. METHODS: We concentrated on the temporal reachability of nodes in temporal brain networks derived from the resting-state functional magnetic resonance imaging (rs-fMRI) of 55 MDD patients and 62 sex-, age-matched healthy controls. Specifically, temporal connectedness and temporal efficiency (TEF) were estimated based on the length of temporal paths between node pairs. Subsequently, the temporal clustering coefficient (TCC) and temporal distance were jointly employed to explore the patterns in which a node's periphery structure affects its reachability. RESULTS: Significantly higher TEF and lower TCC were found in temporal brain networks in MDD. Besides, significant between-group differences of nodal TCC were detected in regions of sensory perception systems. Considering the temporal paths that begin or end at these regions, MDD patients showed several altered temporal distances. CONCLUSION: Our results showed that the temporal reachability of specific brain regions in MDD could be affected as their periphery structures evolve, which may explain the dysfunction of sensory perception systems in the spatiotemporal domain.

2.
Chemosphere ; 286(Pt 1): 131648, 2022 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-34315079

RESUMEN

Aquatic macrophytes have been widely employed for in-situ phytoremediation of cadmium (Cd) polluted sediments. But, little is known about the responses of rhizosphere bacteria and their interspecific interactions to phytoremediation. In this study, the α-diversity, community composition, co-occurrence network and keystone species of sediment bacteria in rhizosphere zones of two typical macrophytes, Hydrilla verticillata and Elodea canadensis, were investigated using 16S rRNA gene high-throughput sequencing. The results showed that after fifty days of phytoremediation, a group of specialized sediment bacteria were assembled in the rhizosphere zones closely associated with different host macrophytes. Rhizosphere micro-environments, i.e., the increases of redox potential and organic matter and the decreases of pH, nitrogen and phosphorus, reduced bacterial α-diversity through niche-based species-sorting process, which in turn reduced interspecific mutualistic relationships. But meanwhile, benefiting from the nutrients supplied from macrophyte roots, more bacterial species survived in the highly Cd-contaminated sediments (50 mg kg-1). In addition, the co-occurrence network revealed that both macrophytes harbored two same keystone bacteria with the high betweenness centrality values, including the family Pedosphaeraceae (genus_unclassified) and genus Parasegetibacter. Their relative abundances were up to 28-fold and 25-fold higher than other keystone species, respectively. Furthermore, these two keystone bacteria were metabolic generalists with vital ecological functions, which posed significant potentials for promoting plant growth and tolerating Cd bio-toxicity. Therefore, the identified keystone rhizobacteria, Pedosphaeraceae and Parasegetibacter, would be potential microbial modulations applied for the future optimization of phytoremediation in Cd-contaminated sediment.


Asunto(s)
Hydrocharitaceae , Biodegradación Ambiental , Cadmio/análisis , ARN Ribosómico 16S/genética , Rizosfera
3.
J Affect Disord ; 296: 622-634, 2022 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-34688026

RESUMEN

RATIONALE/IMPORTANCE: Researches have highlighted communication deficits between resting-state brain networks in major depressive disorder (MDD), as reflected in abnormal functional connectivity (FC). However, it is unclear whether impaired FC is associated with MDD pathology or is simply incidental to MDD symptoms. Moreover, there is no generalized theory to analyze the impact of treatment modalities on MDD. OBJECTIVES: To address the issues, we conducted a systematic review of 49 eligible papers to provide insight into the pathological mechanisms of MDD patients by summarizing resting-state FC alterations involving mood and cognitive abnormalities and the effects of medications on them. RESULTS: Mood disorders in MDD were characterized by abnormal FC between the amygdala, insula, anterior cingulate cortex (ACC), and prefrontal cortex (PFC). Cognitive impairment manifests as deficits in executive function, attention, memory, and rumination, primarily modulated by dysfunction between the fronto-parietal network and default mode network. Especially, we proposed the set of core abnormal FC (CA-FC) contributing to mood and cognitive impairment in MDD, currently including ACC-left precuneus/amygdala, rostral ACC-left dorsolateral PFC, left subgenual ACC-left cerebellar, left PFC- anterior subcallosal, and left precuneus-left pulvinar. After treatment, patients with normalized CA-FC showed remission of depressive symptoms. CONCLUSIONS: We propose a CA-FC set for possible causative principle of MDD, which unifies the FC results from specific, difficult-to-analyze conditions into one outcome set for screening. Furthermore, CA-FC varies from person to person, and the low success rate of a single treatment may be due to the inability to cover too many CA-FC.

4.
Sci Total Environ ; 806(Pt 3): 150467, 2022 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-34592285

RESUMEN

Photochemical transformation of natural organic matter in aquatic environments strongly impacts the environmental behaviors of carbon, nutrients, and pollutants by affecting their solubility, toxicity, bioavailability, and mobility. However, the role of particulate organic matter (POM) in environmental photogeochemistry has received much less attention than that of dissolved organic matter (DOM). In this study, a systematic overview was conducted to summarize the photodissolution and photoflocculation of POM in aquatic systems. The photodissolution of various POM, such as resuspended sediments and algal detritus, could be a potential and important source of DOM in the overlying waters, and these photoreleased DOM were dominated by humic-like components. The photogeochemistry of POM is thought to proceed via direct photochemical reactions and reactive radical-dominated indirect processes. Photodissolution can modify the bioavailability of organic matter and influence the biogeochemical cycling of nutrients, heavy metals, and organic pollutants. In addition, the photo-induced flocculation of DOM to POM could also influence the transport and transformation of organic matter and its associated pollutants. The photochemistry of POM can be significantly influenced by several environmental factors, including irradiation wavelength and intensity, organic matter properties, and radical oxygen species. POM photogeochemistry is one of the most important components of the global cycling of natural organic matter. Further studies regarding photogeochemistry should be conducted to overcome the potential problems arising from the concurrent photodegradation of organic matter and to further develop more filed investigations and analytical methods.

5.
Clin Cancer Res ; 2021 Nov 05.
Artículo en Inglés | MEDLINE | ID: mdl-34740921

RESUMEN

PURPOSE: Immunotherapy offers a second-line option for patients with metastatic urothelial carcinoma (mUC) who failed standard therapy, but the biomarkers for predicting response remain to be explored. This study aims to evaluate the safety, efficacy, and correlative biomarker of toripalimab in patients with previously treated mUC. EXPERIMENTAL DESIGN: Patients with mUC received toripalimab 3 mg/kg Q2W. Clinical response was assessed every 8 weeks by an independent review committee per RECIST v1.1. Tumor PD-L1 expression, tumor mutational burden (TMB), and other biomarkers were evaluated. RESULTS: Among the intention-to-treat population (n = 151), 85% of the patients experienced treatment-related adverse event (TRAE) and 20% experienced grade 3 and above TRAE. The objective response rate (ORR) was 26% with a disease control rate (DCR) of 45%. The median duration of response, progression-free survival (PFS) and overall survival (OS) were 19.7 months (95% CI: 13.9 to NE), 2.3 months (95% CI: 1.8 to 3.6) and 14.4 months (95% CI: 9.3 to 23.1), respectively. Both PD-L1+ and TMB-high (10 mutations/Mb as the cut off) patients had better ORR than PD-L1- patients (42% versus 17%, p = 0.002) and TMB low patients (48% versus 22%, p = 0.014), respectively. The TMB-high group also showed better PFS (12.9 versus 1.8 months, p < 0.001) and OS (not reached versus 10.0 months, p = 0.018) than the TMB-low group. CONCLUSIONS: Toripalimab has demonstrated encouraging clinical activity in the second-line treatment of mUC with a manageable safety profile. PD-L1 expression and TMB were two independent biomarkers in the study.

6.
Sci China Life Sci ; 2021 Nov 10.
Artículo en Inglés | MEDLINE | ID: mdl-34767152

RESUMEN

Timely programmed cell death (PCD) of the tapetum supplying nutrients to microspores is a prerequisite for normal pollen development. Here we identified a unique mutant of rice (Oryza sativa L.), pollen sterility (post), which showed aborted pollens accompanied with extra-large husks. Due to failure of timely PCD of tapetal cells, post exhibited abnormal pollen wall patterning and defective pollen grains. By map-based cloning, we identified a causal gene, POST, encoding a novel protein which is ubiquitously localized in cells. RNA in situ hybridization showed that POST is highly detected in the tapetum and microspores at stages 8 and 9. Transcriptome analysis indicated that POST could function as an important regulator of the metabolic process involved in tapetal PCD. Compared with wild-type rice, post mutant has an increased cell number resulting from elevated expression of cell cycle associated genes in grain husks. Overexpression of POST inhibits grain size in wild type, while appropriate expression of POST in post mutant can recover the seed fertility but has little effect on the large grains, illustrating that fine-tuning of POST expression could be a potential strategy for rice yield improvement. The connection between cell division and cell death conferred by POST provides novel insights into the understanding of the tapetal PCD process.

7.
Anal Chim Acta ; 1186: 339134, 2021 Nov 22.
Artículo en Inglés | MEDLINE | ID: mdl-34756259

RESUMEN

In recent years, single particle inductively coupled plasma mass spectrometry (SP-ICP-MS) has become a powerful tool for biological quantitative analysis. Homogeneous analysis method requires no separation and washing steps, which is suited for the analysis of highly infectious pathogens, so as to reduce the risk of infection during the operation. SARS-CoV-2 spreads all over the world, and its early infection symptoms are similar to influenza, which brings inconvenience to triage. Therefore, developing novel analytical method for simultaneous detection of multiple viral nucleic acids is essential. Taking the advantages of SP-ICP-MS and homogeneous analysis strategy, a SP-ICP-MS homogeneous nucleic acid assay by using gold nanoparticles (Au NPs) and silver nanoparticles (Ag NPs) probes was established for simultaneous sensitive analysis of SARS-CoV-2 and influenza A (H3N2). In the present of target SARS-CoV-2 or H3N2 nucleic acids, corresponding Au NPs or Ag NPs probes form larger aggregates, resulting in increased pulse signal intensity and reduced pulse signal frequency of the corresponding NPs in SP-ICP-MS measurement. In this assay, the reaction system of Au NPs and Ag NPs probes does not interfere with each other, and there was no separation and washing procedure, which facilitates operation, saves the analysis time, and improves the analysis efficiency. The linear range of this method is 5-1000 pmol L-1, with low-level limits of quantification of target nucleic acid. The developed SP-ICP-MS simultaneous homogeneous detection method has a good potential for detecting nucleic acid, protein, cell and other biological samples by changing different modification sequences on the NPs probes.


Asunto(s)
COVID-19 , Gripe Humana , Nanopartículas del Metal , Ácidos Nucleicos , Oro , Humanos , Subtipo H3N2 del Virus de la Influenza A , Espectrometría de Masas , SARS-CoV-2 , Plata
8.
J Plant Physiol ; 267: 153556, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34737128

RESUMEN

The spring shoot leaves are important sites of nitrogen (N) metabolism in citrus trees. Understanding the physiological and metabolic response of the spring shoot leaves under varying N fertilization is fundamental to the fertilization management in citrus orchards. Thus, the processes affecting N composition, the activities of N metabolism related enzymes, and the expression of relevant genes were explored in spring shoot leaves under four N levels (0, 207, 275, 413 g N tree-1 y-1, as N0, N207, N275, N413). The results showed that, compared with N0, N275 significantly increased total N by 24.81%, which was mainly attributed to enhancement of structural N by 30.92%, free amino acid N by 40.91% and nitrate N by 41.33%. The relative expression of nitrate reductase (NR) and glutamate dehydrogenase (GDH) under N275 increased by 19.32% and 73.48%, respectively, compared with that under N0 treatment. Compared with N0 treatment, the NR transcription level under N275 treatment increased by 381%. The relative transcription levels of NADP-GDH and GDH1 also increased with increasing N fertilization. However, compared with that under N275, the relative transcription of GDH2 under N413 treatment was inhibited. Therefore, the transcript abundance of NR, NADP-GDH,GDH1 and GDH2 affected the activities of NR and GDH and thereby contributed to the regulation of N composition in the leaves. In addition, the activities of glutamine synthetase and nitrite reductase were largely unaffected or even declined in the N207, N275 and N413 treatments compared with the N0. This study elucidated the mechanism of primary N metabolism and partitioning in citrus leaves and provided a theoretical basis for N management in citrus orchards.

9.
NPJ Parkinsons Dis ; 7(1): 97, 2021 Nov 09.
Artículo en Inglés | MEDLINE | ID: mdl-34753948

RESUMEN

Here we introduce Local Topological Recurrence Analysis (LoTRA), a simple computational approach for analyzing time-series data. Its versatility is elucidated using simulated data, Parkinsonian gait, and in vivo brain dynamics. We also show that this algorithm can be used to build a remarkably simple machine-learning model capable of outperforming deep-learning models in detecting Parkinson's disease from a single digital handwriting test.

11.
J Chromatogr A ; 1660: 462665, 2021 Nov 02.
Artículo en Inglés | MEDLINE | ID: mdl-34798443

RESUMEN

Cyanuric chloride and 4,4'-diamino-p-terphenyl were adopted as monomers to synthesize poly (4,4'-diamino-p-terphenyl-triazine) (PDT) covalent organic polymer. PDT coated stir bar was prepared and evaluated for the extraction of five phthalate esters (PAEs) with relatively lower logP values (2.7-4.9), including diethyl phthalate, diallyl phthalate, dipropyl phthalate, benzylbutyl phthalate and dibutyl phthalate. It exhibited higher extraction recovery (> 65%) and faster extraction kinetics (50 min vs 240 min) for target PAEs over commercial polydimethylsiloxane coated stir bar. Based on the superior performance, PDT coated stir bar sorptive extraction was combined with high-performance liquid chromatography-diode array detection for trace analysis of five PAEs plasticizers. The limits of detection for target PAEs were 0.04-0.27 µg/L, with the enrichment factors of 54-80-fold. The potential of the method was demonstrated by detecting five target PAEs in Chinese liquor and mineral water samples. No target analytes were detected in Chinese liquor sample, and recoveries of 85.4-109% were obtained for target analytes in spiked liquor samples; trace diethyl phthalate (1.19-2.98 µg/L) and dibutyl phthalate (0.77-0.91 µg/L) were detected in two mineral water samples, with recoveries of 85.4-117% and 87.4-117% respectively in spiked mineral water samples.

12.
Environ Pollut ; 293: 118495, 2021 Nov 13.
Artículo en Inglés | MEDLINE | ID: mdl-34785289

RESUMEN

Black soldier fly, Hermetia illucens L. (Diptera: Stratiomyidae) larvae (BSF larvae or BSFL) offer an environmental-friendly method for degrading antibiotics, such as tylosin (TYL) and enrofloxacin (EF), in swine manure. This study examined the impact of temperature on this process, role of associated microbes, dynamics of resistant genes, and a description of the microbial community associated with the BSF larval gut, how microbes isolated from the BSF larval gut as inoculants impact the process as well as enhance antibiotic digestion, and finally a quantification of antibiotics in BSF larvae fed manure with TYL or EF. Antibiotic degradation in manure was optimized at 28 °C with at least 10% greater than 23 °C and 37 °C. More than 40% reduction in TYL and EF concentrations in the manure occurred when BSF larval gut associated microbes were present. Furthermore, DNA extracted from the gut of non-sterile BSF larvae fed manure with TYL or EF indicated at least two 2-△△Ct fold increase in antibiotic resistance genes for TYL and EF. We identified 250, 4, and 16 unique operational taxa for larvae fed control manure and manure with either TYL or EF. Intestinal microbes isolated from non-sterile larvae fed manure with TYL or EF, were identified, cultured, and examined for their ability to degrade TYL and EF in Luria-Bertani (LB) medium. Three strains (two strains of Enterococcus faecalis and one strain of Proteus mirabilis) resulted in at least 50% TYL or EF degradation within 96 h. Sterile BSF larvae inoculated with P. mirabilis recovered >60% of the degradation ability exhibited by non-sterile larvae. Finally, no TYL residuals were found in 14-d-old larvae, prepupae, or pupae of BSF immatures fed manure containing these antibiotics. While ∼65 µg/g and ∼20 µg/g of EF were found in larval contents and pupal exoskeleton, respectively.

13.
J Nematol ; 532021.
Artículo en Inglés | MEDLINE | ID: mdl-34820628

RESUMEN

Spodoptera litura is a notorious leaf feeding insect pest in the Asia-Pacific region and leads to a significant economic loss in vegetable and field crop production. Entomopathogenic nematodes (EPNs), lethal parasites of insects, are used as biocontrol agents. Yunnan Province in China is a well-known region due to its rich biodiversity. In the present study, a survey of EPNs using the Galleria-baiting technique was conducted in 2017 and 2018 throughout the entire Yunnan province. In total, 789 soil samples were collected from 232 sites, of which 75 samples were positive for EPNs. Phylogenetic analyses of ITS, D2D3 expansion region of the 28S rRNA gene, as well as mitochondrial cytochrome c oxidase subunit I (COI), were performed to identify isolated nematode species and evaluate their genetic diversity. In total, 13, 3, and 58 identified populations belong to Steinernema, Heterorhabditis, and Oscheius, respectively. The phylogenetic relationships of EPN species in the three genera were analyzed with the Neighbor-Joining method. The virulence of the trapped isolates in the genera of Steinernema, Heterorhabditis, and Oscheius against S. litura was evaluated. Ten new indigenous isolates from Steinernema and Heterorhabditis showed prominent virulence to S. litura within 48 hr which is equivalent to that of commercial EPNs populations. The present study provides background information on indigenous EPN resources for S. litura control in Asia-Pacific region.

15.
Front Oncol ; 11: 764065, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34804967

RESUMEN

Objective: Stage I and II cervical cancer with pelvic and/or para-aortic lymph node (LN) metastases are upstaged to stage IIIC under the new FIGO 2018 staging system, and radical chemoradiotherapy was recommended. But heterogeneity in outcome existed in this group of patients. We conducted this retrospective analysis to evaluate the heterogeneity of these patients and tried to provide a more detailed classification to reflect the prognosis and guide the treatment. We also evaluated the efficacy and toxicity of surgery followed by sequential chemoradiotherapy in this cohort. Methods: Early-stage cervical cancer with LN involvement that had radical hysterectomy followed by sequential chemoradiotherapy were retrospectively analyzed. Survival analyses were conducted to identify the prognostic factors. Results: A total of 242 patients were included in the study; 64 (26.4%) patients had treatment failure, and 51 (21.1%) died. Pathology, T stage, the number of pathologic LN (pLN), and neoadjuvant chemotherapy or not were independent prognostic factors for disease-free survival and overall survival (OS). Patients with T1N < 3 pLN had significantly better survival than T2N < 3 pLN/T1-2 N≥ 3 pLN, with failure rates of 11.6% and 35.8% in each group; and 5 year OS was 92% and 62%, respectively (P = 0.000). About 1.5% of the patients discontinued radiotherapy, and 14.1% had G3-4 hematological toxic effects during radiotherapy; 1.7% developed G2-3 lower limb edema, and 2.9% developed severe urinary toxicity. Conclusion: Nodal involvement alone is inadequate as the sole pathologic factor to predict survival in early-stage cervical cancer. The combination of tumor and node subcategory provides better prognostic discrimination.

16.
Ann Transl Med ; 9(19): 1492, 2021 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-34805354

RESUMEN

Background: Through previous studies and clinical practice, we have found that real-time ultrasound-guided (UG) spinal anesthesia (SA) and traditional landmark-guided (LG) SA each require a different minimum local anesthetic dose (MLAD) of ropivacaine. For this study, we used Dixon's up-and-down sequential method to analyze and compare the MLAD of different ropivacaine concentrations required for the UG and LG SA methods. Methods: A total of 120 patients undergoing knee surgery were consecutively recruited and randomly divided into four groups (30 patients per group). These groups were categorized as follows: Group I: high ropivacaine ultrasound-guided (HRUG), Group II: low ropivacaine ultrasound-guided (LRUG), Group III: high ropivacaine landmark-guided (HRLG), and Group IV: low ropivacaine landmark-guided (LRLG). SA was established by a bolus administration of up-and-down doses of 0.75% or 0.5% plain ropivacaine. Initial doses of 16, 18, 12, and 14 mg were administered to groups I-IV, and after that, increased or decreased by 1.5 mg according to dose effectiveness. Upon identifying the intervertebral puncture level, a lumbar X-ray was performed with metal markers, and actual radiographic findings were identified and compared to the initial markings. Results: For UG groups, the MLAD in the LRUG group was significantly higher than in the HRUG group [20.192 mg (95% CI, 19.256-21.174) versus 17.176 mg (95% CI, 16.276-18.124), respectively; P<0.001]. For LG groups, the MLAD in the LRLG group was significantly higher than in the HLRG group [14.478 mg (95% CI, 13.364-15.500) versus 13.201 mg (95% CI, 11.959-14.571), respectively; P=0.047]. When comparing both high ropivacaine groups (HRGs: I/III) to the low ropivacaine groups (LRGs: II/IV), we found that both UG subgroups (I/II) had a significantly higher MLAD than LG subgroups (III/IV) (P<0.001). US identified L4-5 in up to 90% of cases. Comparatively, palpation was successful in only 33.3% of patients. The rates of cephalad localization by US and palpation were 6.67% vs. 66.67%, respectively (P=0.002). Conclusions: We found a higher MLAD of ropivacaine was required for UG SA at the L4-5 level due to the method providing a more accurate (less cephalad) localization than traditional LG SA. Trial Registration: Chinese Clinical Trial Registry ChiCTR2000033158.

17.
Int J Mol Sci ; 22(21)2021 Oct 27.
Artículo en Inglés | MEDLINE | ID: mdl-34769051

RESUMEN

As a class of difurancoumarin compounds with similar structures, aflatoxins (AF) are commonly found in the environment, soil, and food crops. AF pose a serious threat to the health of humans, poultry, and livestock. This study aimed to investigate the neuroprotective effect and detailed mechanism of aloin on hepatic injury induced by subchronic AFB1 in rats. The result showed that aloin could significantly inhibit the decrease in food intake, body weight growth, immune organ index, and serum albumin content caused by long-term AFB1 exposure. Meanwhile, aloin reduced the level of serum liver function and improved renal swelling and pathological changes of liver tissue. Aloin could also inhibit liver lipid peroxidation and improve liver antioxidant capacity. Further investigation revealed that aloin inhibited the activity and expression of hepatic CYP1A2 and CYP3A4 and down-regulated IL-1ß expression in subchronic AFB1-induced liver injury rats. The above study demonstrated that aloin played an important role in blocking or delaying the development process of subchronic AFB1-induced hepatotoxicity. Therefore, aloin is considered to have a potential role as a protective agent against AFB1.

18.
Cell Rep ; 37(6): 109981, 2021 Nov 09.
Artículo en Inglés | MEDLINE | ID: mdl-34758299

RESUMEN

Memory T cells exhibit considerable diversity that determines their ability to be protective. Here, we examine whether changes in T cell heterogeneity contribute to the age-associated failure of immune memory. By screening for age-dependent T cell-surface markers, we identify CD4 and CD8 memory T cell subsets that are unrelated to previously defined subsets of central and effector memory cells. Memory T cells expressing the ecto-5'-nucleotidase CD73 constitute a functionally distinct subset of memory T cells that declines with age. They resemble long-lived, polyfunctional memory cells but are also poised to display effector functions and to develop into cells resembling tissue-resident memory T cells (TRMs). Upstream regulators of differential chromatin accessibility and transcriptomes include transcription factors that facilitate CD73 expression and regulate TRM differentiation. CD73 is not just a surrogate marker of these regulatory networks but is directly involved in T cell survival.

19.
Mol Cancer Ther ; 2021 Nov 23.
Artículo en Inglés | MEDLINE | ID: mdl-34815360

RESUMEN

Triple-negative breast cancer (TNBC) is a subtype of breast cancer lacking targetable biomarkers. TNBC is known to be most aggressive, and when metastatic is often drug resistant and uncurable. Biomarkers predicting response to therapy improve treatment decisions and allow personalized approaches for TNBC patients. This study explores sulfated glycosaminoglycan (sGAG) levels as a predictor of TNBC response to platinum therapy. sGAG levels were quantified in three distinct TNBC tumor models including cell line-derived, patient-derived xenograft (PDX) tumors, and isogenic models deficient in sGAG biosynthesis. The in vivo antitumor efficacy of Triplatin, a sGAG-directed platinum agent, was compared in these models to the clinical platinum agent, carboplatin. We determined that >40% of TNBC PDX tissue microarray samples have high levels of sGAGs. The in vivo accumulation of Triplatin in tumors as well as antitumor efficacy of Triplatin positively correlated with sGAG levels on tumor cells, whereas carboplatin followed the opposite trend. In carboplatin-resistant tumor models expressing high levels of sGAGs, Triplatin decreased primary tumor growth, reduced lung metastases, and inhibited metastatic growth in lungs, liver, and ovaries. sGAG levels served as a predictor of Triplatin sensitivity in TNBC. Triplatin may be particularly beneficial in treating patients with chemotherapy-resistant tumors who have evidence of residual disease after standard neoadjuvant chemotherapy. More effective neoadjuvant and adjuvant treatment will likely improve clinical outcome of TNBC.

20.
J Colloid Interface Sci ; 608(Pt 2): 1257-1267, 2021 Oct 24.
Artículo en Inglés | MEDLINE | ID: mdl-34739989

RESUMEN

In this study, a series of one-dimensional (1D)/two-dimensional (2D) heterostructure hybrids were fabricated through the in situ growth of a Co and Ni bimetallic zeolitic imidazolate framework (CoNi-ZIF) around N-doped carbon nanotubes (N-CNTs). The hybrids were further exploited as effective supercapacitor materials. The N-CNTs were prepared by carbonizing a mixture of glucose and the melamine-cyanuric acid complex at a high temperature (900 °C) under N2 atmosphere and applied as the template for the in situ synthesis of CoNi-ZIF nanosheets (NSs). The 1D N-CNTs in the hybrids can act as the high-way for charge transfer to boost the faradaic reactions. Changing the usage of metal precursors not only provided abundant redox reaction sites in 2D CoNi-ZIF NSs but also modulated the microstructures and chemical components of the hybrids. The integration of the features of N-CNTs and CoNi-ZIF NSs can result in a synergistic effect between N-CNTs and CoNi-ZIF NSs. Therefore, the obtained CoNi-ZIFs and N-CNTs hybrid (CoNi-ZIF@N-CNT) exhibited superior electrochemical capacitive performance. Comparison revealed that the CoNi-ZIF@N-CNT-2 hybrid, which was prepared with a 1:1 mass ratio of Co(NO3)2·6H2O and Ni(NO3)2·6H2O, displayed the largest specific capacitance of 1118F g-1 at 1 A g-1, which was higher than the capacitance of most reported metal-organic framework (MOF)-based supercapacitor electrodes. Moreover, the asymmetric supercapacitor based on the CoNi-ZIF@N-CNT-2 electrode exhibited a high energy density of 51.1 Wh kg-1 at the power density of 860.1 W kg-1 and good cycle stability. This work can provide a facile and effective way for the fabrication of heterostructured 1D/2D nanostructures based on 2D MOFs for advanced energy storage.

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