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5.
6.
Nat Biotechnol ; 17(8): 733, 1999 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-10429262
10.
Mol Gen Genet ; 246(4): 426-36, 1995 Feb 20.
Artículo en Inglés | MEDLINE | ID: mdl-7891656

RESUMEN

The PIF1 and MRS2 gene products have previously been shown to be essential for mitochondrial DNA maintenance at elevated temperatures and mitochondrial group II intron splicing, respectively, in the yeast Saccharomyces cerevisiae. A multicopy suppressor capable of rescuing the respiratory deficient phenotype associated with null alleles of either gene has been isolated. This suppressor is a nuclear gene that was called RIM2/MRS12. The RIM2/MRS12 gene encodes a predicted protein of 377 amino acids that is essential for mitochondrial DNA metabolism and proper cell growth. Inactivation of this gene causes the total loss of mitochondrial DNA and, compared to wild-type rhoo controls, a slow-growth phenotype on media containing glucose. Analysis of the RIM2/MRS12 protein sequence suggests that RIM2/MRS12 encodes a novel member of the mitochondrial carrier family. In particular, a typical triplicate structure, where each repeat consists of two putative transmembrane segments separated by a hydrophilic loop, can be deduced from amino acid sequence comparisons and the hydropathy profile of RIM2/MRS12. Antibodies directed against the aminoterminus of RIM2/MRS12 detect this protein in mitochondria. The function of the RIM2/MRS12 protein and the substrates it might transport are discussed.


Asunto(s)
Proteínas Portadoras/genética , ADN de Hongos/metabolismo , Mitocondrias/genética , Proteínas Nucleares/genética , ARN de Hongos/metabolismo , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Secuencia de Aminoácidos , Mapeo Cromosómico , Clonación Molecular , Proteínas Fúngicas/genética , Mitocondrias/metabolismo , Datos de Secuencia Molecular , Proteínas de Transporte de Nucleótidos , Saccharomyces cerevisiae/metabolismo , Homología de Secuencia de Aminoácido , Supresión Genética
11.
Trends Biotechnol ; 13(2): 42-4, 1995 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-7765992
13.
Curr Genet ; 24(4): 301-6, 1993 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-8252639

RESUMEN

Disruption of the nuclear MRS2 gene (mrs2-1 mutation) causes a strong pet- phenotype in strains with mitochondrial group II introns, and a leaky pet- phenotype in strains without group II introns. MRS3 and MRS4, the genes for two mitochondrial-solute carrier proteins, can suppress both phenotypes when present in high-copy-number plasmids. In order to search for further multicopy suppressors of the mrs2-1 mutant phenotype, an yeast genomic DNA library, MW90, was constructed in YEp351 from a strain deleted for the MRS2, MRS3 and MRS4 genes. Ten different Sau3A DNA fragments that act as multicopy suppressors of the mrs2-1 respiratory-deficient phenotype were isolated from this library. Some of the newly isolated genes suppress the pet- phenotypes of mrs2-1 cells in strains with and without mitochondrial group II introns. Other genes, however, are suppressors only for the mitochondrial intron-less strains. This supports the notion that the MRS2 gene product is bifunctional i.e., it is essential for the splicing of group II introns and is also involved in processes of mitochondrial biogenesis other than RNA splicing.


Asunto(s)
Genes Supresores , Intrones , Empalme del ARN/genética , Saccharomyces cerevisiae/genética , Genes Fúngicos , Prueba de Complementación Genética , Mutación , Fenotipo , Mapeo Restrictivo , Transformación Genética
14.
Curr Genet ; 23(1): 15-8, 1993 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-8428379

RESUMEN

This communication reports on a single-copy gene of Saccharomyces cerevisiae which is homologous to the rat ribosomal protein gene L21. The yeast and the rat genes show 59% identity in DNA sequences and in the predicted protein sequences. This yeast gene is, therefore, assumed to code for an as yet unassigned ribosomal protein (URP1). The URP1 open reading frame is 480 nucleotides long and can encode a protein of about M(r) 18,200. Like most of the other known ribosomal protein genes, URP1 is interrupted by an intron in its 5' terminal part and it is preceded by upstream sequence elements which usually regulate transcription of these genes. Northern blot analysis reveals that the URP1 gene is actually expressed in vivo.


Asunto(s)
Proteínas Fúngicas/genética , Genes Fúngicos , Proteínas Ribosómicas/genética , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Northern Blotting , ADN de Hongos , Datos de Secuencia Molecular , Ratas , Mapeo Restrictivo , Homología de Secuencia de Aminoácido
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