Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 25
Filtrar
1.
Artículo en Inglés | MEDLINE | ID: mdl-33731578

RESUMEN

BACKGROUND AND AIMS: Occult hepatitis C infection is defined as the presence of hepatitis C virus RNA in peripheral blood mononuclear cells (PBMC) ± hepatocytes in the absence of HCV-RNA in serum. It has been a lot of debate and controversy in recent years and not discussed well. This issue has not been discussed or investigated in Egypt, especially in patients on hemodialysis in Ismailia. This study is the first one to investigate the prevalence of occult HCV infection in large populations of chronic hemodialysis (CHD) patients in Ismailia, Egypt. METHODS: Our study is cross-sectional analytic and included 204 CHD patients; who are negative for HCV infection. Sensitive commercial real-time assay was used to detect HCV-RNA in PBMC. In our study, the presence of genomic HCV-RNA in PBMCs of all these patients was detected by real-time PCR. On the other hand, 22 patients on hemodialysis with an established diagnosis of chronic hepatitis C virus infection were included as a control group and examined by real-time PCR was used to evaluate HCV infection. RESULTS: Occult HCV infection is defined as the presence of HCV-RNA in PBMNCs in patients on chronic hemodialysis, and it was found in 14/204 (7%) of the patients. Patients who were on CHD for a longer time are susceptible to occult HCV infection, and their mean alanine aminotransferase levels are significantly higher during the last 3 months before study entry. In comparison, chronic HCV patients have elevated bilirubin, aspartate transferase and alanine transferase than occult HCV infection. CONCLUSIONS: The prevalence of occult HCV infection was 7% in our CHD patients. No available data are showing the virulence of this form of virus. However, further studies in other geographic populations with high HCV endemicity are needed to clarify the significance of occult HCV infection in these patient groups, in addition to test for the presence of negative antigenomic strand to confirm or disconfirm the reliability of occult HCV.

2.
J Basic Microbiol ; 61(4): 330-338, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33599337

RESUMEN

Microalgae have received continued attention as a potential source for biofuel production. However, the lack of suitable strains that provide a lipid-rich biomass and tolerate harsh condition inhibits their industrial application. This report describes an effort to transform Synechocystis sp. with genes encoding acetyl-CoA carboxylase (ACC), a key regulatory enzyme in the lipogenesis pathway, from the white mustard plant (Sinapis alba) and the bacterium Escherichia coli DH5α using chitosan nanoparticles. Although a recombinant plasmid encoding S. alba ACC failed to express, successful transformation was achieved with a recombinant plasmid encoding E. coli DH5α ACC. The successful transformant, Synechocystis sp. PAK13, exhibited increased ACC expression compared with its wild-type parent (11.8 vs. 7.2 ng), which significantly increased its lipid content (by 3.6-fold). Synechocystis sp. PAK13 also exhibited a significant (20%) reduction in photosynthetic pigments, a 1.52-fold higher glucose content and a 3.5-fold lower sucrose content than the wild-type. In conclusion, this report introduces a useful strategy to overexpress the ACC gene in microalgae, creating strains with improved lipid production that are suited to industrial applications.

3.
Environ Sci Technol ; 2020 Dec 08.
Artículo en Inglés | MEDLINE | ID: mdl-33291866

RESUMEN

The study investigates the spatial pattern of black carbon (BC) at a high spatial resolution in Augsburg, Germany. Sixty two walks were performed to assess the concentrations of equivalent black carbon (eBC), ultraviolet particulate matter (UVPM), and equivalent brown carbon (eBrC) in different seasons and at different times of the day with a mobile platform (i.e., trolley). Along with BC measurements, images of street microenvironments were recorded. Meteorological parameters, including temperature, relative humidity, and wind speed, were monitored. The BC concentrations showed significant spatial heterogeneity and diurnal variations peaking in the morning and at night. The highest BC concentrations were observed near dense traffic. The correlations between BC and street views (buildings, roads, cars, and vegetation) were weak but highly significant. Moreover, meteorological factors also influenced the BC concentration. A model based on street view images and meteorological data was developed to examine the driving factors of the spatial variability of BC concentrations at a higher spatial resolution as different microenvironments based on traffic density. The best results were obtained for UVPM and eBC (71 and 70% explained variability). eBrC (53%), to which other sources besides road traffic can also make significant contributions, is modeled less well.

4.
J Genet Eng Biotechnol ; 18(1): 80, 2020 Dec 02.
Artículo en Inglés | MEDLINE | ID: mdl-33263861

RESUMEN

BACKGROUND: Plasmid propagation in recombination strains such as Escherichia coli DH5α is regarded as a beneficial instrument for stable amplification of the DNA materials. Here, we show trans-conjugation of pGEM-T cloning vector (modified Promega PCR product cloning vector with tra genes, transposable element (Tn5)) and M13 sequence via α-complementation mechanism in order to activate ß-D-galactosidase gene in DH5α strain (non-lactose-fermenting host). RESULTS: Trans-conjugation with pGEM-T allows correction of LacZ gene deletion through Tn5, and successful trans-conjugants in DH5α host cells can be able to produce active enzyme, thus described as lactose fermenting strain. The intracellular ß-galactosidase was subjected to precipitation by ammonium sulfate and subsequently gel filtration, and the purified enzyme showed a molecular weight of approximately 72-kDa sodium dodecyl sulfate-polyacrylamid gel electrophoresis. The purified enzyme activity showed an optimal pH and temperature of 7.5 and 40 °C, respectively; it had a high stability within pH 6-8.5 and moderate thermal stability up to 50 °C. CONCLUSION: Trans-conjugant of E. coli DH5α- lacZ∆M15 was successfully implemented. UV mutagenesis of the potent trans-conjugant isolate provides an improvement of the enzyme productivity. The enzymatic competitive inhibition by D-galactose and hydrolysis of lactose at ambient temperatures could make this enzyme a promising candidate for use in the dairy industry.

5.
Life Sci ; 265: 118818, 2020 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-33275985

RESUMEN

AIM: Proton pump inhibitors (PPIs) are widely used drugs recently linked to chronic kidney disease. However, the invloved mechanisms remained elusive. Since defective autophagy is identified as a new culprit in the pathogenesis of diabetic nephropathy (DN), we aimed to trace the link of autophagy blockade by PPIs to the progression of DN with and without the standard therapy of metformin and enalapril. MAIN METHODS: Male CD1 albino mice (20-25 g) were randomly assigned to normal control or diabetic mice. Diabetes was induced by intraperitoneal streptozotocin (35 mg/kg) injection combined with high fat diet. DN mice were randomized to receive vehicle, lansoprazole (5 mg/kg), metformin (200 mg/kg), lansoprazole + metformin, metformin + enalapril (0.5 mg/kg) or the three drugs together, orally daily for four weeks. At the study end, albuminuria, fasting plasma glucose, HbA1c, renal functions and malondialdehyde were assessed. Renal tissues were examined microscopically, and autophagic changes were evaluated by immunohistochemical detection of LC3-II and p62. KEY FINDINGS: Consistent with autophagic blockade, lansoprazole increased both LC3II and p62 in the glomerular and tubular cells. This was associated with impaired creatinine clearance and renal functions, enhanced albuminuria, oxidative stress and augmented DN histopathological changes. Opposite effects on autophagy markers were observed by single or combined treatment of metformin with enalapril; which also ameliorated glycemic control and signs of DN. This improvement was mitigated by combination with lansoprazole. SIGNIFICANCE: Autophagy blockade by lansoprazole augmented diabetic nephropathy and opposed the reno-protective effects of metformin and enalapril. The use of PPIs in diabetes should be considered with great caution.

6.
Sci Rep ; 10(1): 21830, 2020 Dec 11.
Artículo en Inglés | MEDLINE | ID: mdl-33311527

RESUMEN

The phase stability and microstructure of ZrO2-5CaO and ZrO2-24MgO mixed coating (wt%) by air plasma spraying on 304 stainless steel substrates were investigated. A Ni-5Al (wt%) metallic bond coating was firstly sprayed between the substrate and the ceramic top layer. The results were compared with the individual coatings of ZrO2-5CaO and ZrO2-24MgO for a better understanding of the correlation between their microstructures and mechanical properties. Mixed zirconia coating was found to have a mixture of cubic and tetragonal phases that stabilized under different plasma spray conditions. Microscopic observations and elemental composition analysis of as-sprayed mixed coating showed that modified ceramic-matrix grains had been formed. Microsized ZrO2-5CaO particles were embedded in the matrix grain creating an intragranular microstructure. Results indicated that ceramic-matrix grains provided a diffusion barrier for the growth of oxides induced stress near and onto the bond layer that reduced cracks, thereby overcoming the top delamination of the ceramic coating. Moreover, disparity in wear resistance and microhardness behavior of the coatings was influenced by initial feedstock powder and matrix microstructures. Improvement in the wear resistance of the mixed zirconia coating was attributed to a decrease in oxide content, which resulted in an increase in intersplat cohesive strength.

7.
Exp Clin Transplant ; 18(6): 659-670, 2020 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-32552624

RESUMEN

The BK polyomavirus was isolated in 1971; it has been a significant risk factor for both graft dysfunction and failure in renal transplant recipients. So far, no specific treatment option has been available for effective treatment or prophylaxis for BK virus infections. Although the use of heavy immunosuppression has been the main risk factor for BK virus infection, other risk factors are equally important, including elderly recipients, prior rejection episodes, male sex, human leukocyte antigen mismatching, prolonged cold ischemia time, pretransplant BK virus serostatus, and ureteral stenting. Regular follow-up for BK virus infections according to each institution's policy has been, so far, effective in detecting patients with BK virus viremia and consequently preventing allograft loss. The mainstay of management continues to be reduction of immunosuppression. However, newer options are providing new insights, such as cellular immunotherapy. In this review, we will address the diagnosis, screening, new diagnostic tools, and updated management of BK virus infections.

8.
Sci Total Environ ; 722: 137632, 2020 Jun 20.
Artículo en Inglés | MEDLINE | ID: mdl-32199355

RESUMEN

Generally, there are only a few fixed air quality monitoring stations installed in villages or rural areas and only a few studies on small-scale variations in air pollution have been described in detail, which make it difficult to estimate human exposure in such environments and related adverse health effects. Moreover, biomass combustion can be an important source of air pollution in rural areas, comparable to vehicle and industrial emissions in urban planning. And their air pollutants are mainly affected by local sources. For this reason, a survey on rural air pollution was carried out in this study. Therefore, portable, battery-powered monitoring devices were used to measure particulate matter (PM10, PM2.5, PM1, particle number concentration, and black carbon) in order to study air quality in rural communities. The focus of the investigations was to explore the application of mobile monitoring equipment in small-scale environments, compare the differences in rural air pollutants between two neighboring villages in two countries, and the identification of pollution hotspots. The measurements were carried out in November 2018 in two villages on the German-Czech border. Over a period of four days, 21 mobile measurements along fixed routes were carried out simultaneously at both locations. The analysis of the data revealed significant differences in PN and PM concentrations in rural air pollutants between the two countries. The spatial and temporal distribution of air pollution hotspots in the Czech village was higher than that in the German village. The relationships between the measurement parameters were weak but highly significant and the meteorological parameters can effect air pollution. Overall, the results of this study show that mobile measurements are suitable for effectively recording and distinguishing spatial and temporal characteristics of air quality.

9.
Ann Plast Surg ; 84(5): 518-524, 2020 05.
Artículo en Inglés | MEDLINE | ID: mdl-31800559

RESUMEN

INTRODUCTION: Hypertrophic scars are fibroproliferative disorders, seen after burn, trauma, and/or surgery. We aimed to compare the clinical and histopathological results of 1064-nm Nd:YAG laser and combined intense pulsed light and radiofrequency in the treatment of hypertrophic scars. METHODS: Fifty patients with hypertrophic scars were included in this prospective, randomized study. Twenty-five patients were treated with Nd:YAG laser and 25 patients with combined intense pulsed light and radiofrequency (E-light). The scars were evaluated at baseline, during and at 3 months after the final treatment session using the Vancouver scar scale. Biopsy specimens from scars were obtained before, during, and 3 months after the final treatment session and were stained with hematoxylin and eosin stain, Masson's trichrome stain, and immunostaining procedures for collagen I, collagen III, and TGF-ß1. RESULTS: Significant improvements in the total Vancouver scar scale scores before and after the treatment in both groups (P < 0.001); however, a significant difference between both groups (P < 0.001), regarding the E-light, which showed better response than Nd:YAG laser. Hematoxylin and eosin and Masson's trichrome staining showed arrangement and thinning of collagen bundles and reduction in collagen density by in both groups, but the collagen bundles thinning and parallelism were more obvious in the E-light group. Significant decrease in the concentration of collagen I, collagen III, and TGF-ß1 in the E-light group as compared with the laser group (P = 0.005, P = 0.003 and P < 0.001, respectively). CONCLUSIONS: Both modalities were successful in the treatment of hypertrophic scars; however, a significant improvement in the clinical and histopathological findings was detected with the E-light method.

10.
Hepatol Int ; 13(6): 706-714, 2019 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-31515739

RESUMEN

BACKGROUND/PURPOSE OF THE STUDY: Worldwide and national efforts are directed against eradication of HCV. The introduction of direct-acting antivirals (DAAs) has changed dramatically the outcome of HCV treatment. In spite of the Food and Drug Administration approval of the oral drugs sofosbuvir (SOF) and ledipasvir (LED) for the treatment of HCV in adolescents more than or equal to 12 years old, sufficient real-world experience is still lacking. The aim of this study was to assess the safety and efficacy of the generic SOF/LED fixed-dose combination 400/90 (400 mg SOF + 90 mg LED) for the treatment of adolescents and children (9-12 years) with chronic hepatitis C (CHC). METHODS: In this prospective observational study, 100 cases of genotype 4 CHC were recruited consecutively from those fulfilling the inclusion and exclusion criteria. All cases received the generic fixed-dose combination SOF/LED (400/90), one tablet daily for 12 weeks. All clinical, laboratory, and virologic characteristics were evaluated at base line, and week (W) 2, 4, 8, and 12 of therapy and W12 post-treatment (SVR12). RESULTS: Recruited children (9-12) and adolescents weighed 28-83 and 31-90 kg, respectively. Eighty cases were naïve and 20 cases were pegylated interferon/ribavirin treatment-experienced. Very rapid virologic response (vRVR) at W2 was 96%, while at W4 response rate was 100% and maintained till the end of treatment and at W12 post-treatment (SVR12). All reported side effects were mild and did not lead to treatment termination and disappeared at W12 post-treatment. CONCLUSION: The generic SOF/LED fixed-dose combination is safe and effective in children, 9-12 years, and adolescents with vRVR rate of 96%, 100% EOT response and SVR12.


Asunto(s)
Antivirales/uso terapéutico , Bencimidazoles/uso terapéutico , Fluorenos/uso terapéutico , Hepatitis C Crónica/tratamiento farmacológico , Uridina Monofosfato/análogos & derivados , Administración Oral , Adolescente , Servicios de Salud del Adolescente , Antivirales/administración & dosificación , Bencimidazoles/administración & dosificación , Niño , Servicios de Salud del Niño , Esquema de Medicación , Egipto , Femenino , Fluorenos/administración & dosificación , Genotipo , Hepatitis C Crónica/genética , Humanos , Masculino , Estudios Prospectivos , Sofosbuvir , Resultado del Tratamiento , Uridina Monofosfato/administración & dosificación , Uridina Monofosfato/uso terapéutico
11.
Inorg Chem ; 58(12): 7989-7996, 2019 Jun 17.
Artículo en Inglés | MEDLINE | ID: mdl-31135144

RESUMEN

Layered titanates with one-dimensional (1D) shapes have been an important class of nanomaterials due to their combination of 1D and 2D fascinating properties. Among many layered titanates, lepidocrocite-type layered titanates have significant advantages such as superior intercalation and exfoliation properties, while the synthesis of the 1D-shape forms is still challenging. Here, we report on a facile one-pot hydrothermal conversion of a lepidocrocite-type layered titanate into the corresponding nanowire-shape form. The reaction mechanism involves the decomposition of the starting layered titanate into 1D small segments which assemble into the nanowire. This new nanowire shows properties resulting from the combination of 1D and 2D nanostructural features, excellent cation exchange ability, and high photoinduced charge separation and photocatalytic efficiency. As a demonstration, we evaluate the nanowire as a sequestrating material capable of collecting toxic cations, like Cd2+, from water and photoreducing them (immobilizing them tightly). We find that the nanowire shows an efficient and ultrafast photoimmobilization activity, whereas the starting layered titanate and a benchmark TiO2 photocatalyst (P25) show no activity under the identical conditions. The photoimmobilization rate (within 1 min) is considerably faster than the cation exchange rates reported for state-of-the-art cation exchangers (with no photoimmobilization ability). The nanowire used for photoimmobilization reactions is easily recovered from water by decantation, showing the possible practical use for safe disposal of toxic cations in the environment.

12.
Sensors (Basel) ; 19(7)2019 Apr 08.
Artículo en Inglés | MEDLINE | ID: mdl-30965651

RESUMEN

The smartphone market is rapidly spreading, coupled with several services and applications. Some of these services require the knowledge of the exact location of their handsets. The Global Positioning System (GPS) suffers from accuracy deterioration and outages in indoor environments. The Wi-Fi Fingerprinting approach has been widely used in indoor positioning systems. In this paper, Principal Component Analysis (PCA) is utilized to improve the performance and to reduce the computation complexity of the Wi-Fi indoor localization systems based on a machine learning approach. The experimental setup and performance of the proposed method were tested in real indoor environments at a large-scale environment of 960 m² to analyze the performance of different machine learning approaches. The results show that the performance of the proposed method outperforms conventional indoor localization techniques based on machine learning techniques.

13.
Nanoscale ; 10(26): 12398-12406, 2018 Jul 09.
Artículo en Inglés | MEDLINE | ID: mdl-29926045

RESUMEN

Ordered mesoporous nitrogen-doped carbon (OMNC) materials are considered as the most promising material for supercapacitors. In this study, a highly ordered two-dimensional (2D) hexagonal mesostructured polymer was synthesized through a facile assembly of triblock polymer micelles and phenol-melamine/formaldehyde resin via an organic-organic assembly process in aqueous solution. After calcination, the novel OMNC materials with 2D hexagonal mesostructures were obtained. By further KOH activation, the surface area and the porosity of the OMNC significantly improved, and the internal mesoporous structures were maintained. The activated OMNC-800A displayed a specific capacitance as high as 475.75 F g-1 at 0.5 A g-1 with an outstanding cycling stability (over 100% capacitance retention during 2000 cycling tests at 100 mV s-1). These results confirm that the tubular mesochannels inside the OMNC are very beneficial in providing an accessible path for diffusion of the electrolyte, thereby improving the specific capacitance of OMNC at a high current density.

14.
Am J Orthod Dentofacial Orthop ; 152(3): 336-347, 2017 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-28863914

RESUMEN

INTRODUCTION: The aim of this study was to assess the 3-dimensional soft tissue changes in growing Class III patients with maxillary deficiency associated with 2 bone-anchored maxillary protraction protocols in relation to an untreated control group of Class III patients. METHODS: Growing skeletal Class III patients between the ages of 10 and 14 years participated in this study. In group 1 (n = 10), skeletally anchored facemasks were used with miniplates placed at the zygomatic buttress. In group 2 (n = 10), the patients were treated with Class III elastics extending from infrazygomatic miniplates in the maxilla to symphyseal miniplates in the mandible. Group 3 (n = 10) was an untreated control group. Three-dimensional stereophotogrammetry images were acquired before and after treatment, and then superimposed and analyzed. In addition, lateral cephalometric radiographs were analyzed. RESULTS: The maxilla moved forward significantly in groups 1 and 2 compared with the untreated control group (group 1, 4.87 mm; group 2, 5.81 mm). The 3-dimensional soft tissue analysis showed significant treatment effects; the major changes were observed in the upper lips, cheeks, and middle of the face, which had a significant positive sagittal displacement in both treatment groups. The lower lip and chin area showed significant negative sagittal changes that indicated that the soft tissue growth in this area was restrained with backward displacement especially in group 1 more than in group 2. CONCLUSIONS: The 2 bone-anchored maxillary protraction protocols effectively improved the Class III concave soft tissue profile.


Asunto(s)
Cara/patología , Maloclusión de Angle Clase III/terapia , Métodos de Anclaje en Ortodoncia/métodos , Técnica de Expansión Palatina , Mejilla/diagnóstico por imagen , Mejilla/patología , Niño , Protocolos Clínicos , Tomografía Computarizada de Haz Cónico , Cara/diagnóstico por imagen , Femenino , Humanos , Imagenología Tridimensional/métodos , Labio/diagnóstico por imagen , Labio/patología , Masculino , Maloclusión de Angle Clase III/diagnóstico por imagen , Maloclusión de Angle Clase III/patología , Resultado del Tratamiento
15.
Gastroenterol Res Pract ; 2017: 9089068, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28588613

RESUMEN

The standard-of-care treatment for biliary atresia (BA) is surgical restoration of bile flow by Kasai portoenterostomy. We aimed to study serum interleukin- (IL-) 12p40, a natural antagonist for the proinflammatory IL-12p70, and its relation to surgical outcomes of BA. The study included 75 infants with neonatal cholestasis: BA group (n = 25), non-BA cholestasis group (n = 30), and neglected BA group (n = 20), in addition to thirty healthy neonates serving as controls. IL-12p40 was measured by ELISA in all individuals and a second assessment was performed 3 months postoperatively in the BA group. The surgical outcomes were classified as successful (bilirubin ≤ 2 mg/dl) or failed (bilirubin > 2 mg/dl). IL-12p40 was higher in BA compared to that in the non-BA and control groups (P values were 0.036 and <0.0001, resp.) but comparable to that in the neglected BA group. Preoperative IL-12p40 levels in BA patients were significantly higher in successful Kasai compared with failed Kasai and a cutoff level of 547.47 pg/ml could predict the successful outcome with 87.5% sensitivity and 82.4% specificity. Three-month postoperative IL-12p40 tended to decrease in both the successful and failed groups. In conclusion, preoperative serum IL-12p40 is a potential predictor of Kasai outcome. Serial postoperative measurements may anticipate the failure of an initially successful operation, hence the need for liver transplantation.

16.
Am J Orthod Dentofacial Orthop ; 151(6): 1092-1106, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-28554455

RESUMEN

INTRODUCTION: The aim of this study was to evaluate dentoalveolar and arch dimension changes in 2 miniplate-anchored maxillary protraction protocols in relation to an untreated control group using 3-dimensional digital models. METHODS: Thirty growing Class III subjects with maxillary deficiency in the late mixed or early permanent dentition phase were randomly divided into 3 groups. In group 1 (n = 10), patients were treated with skeletally anchored facemasks anchored with miniplates placed at the zygomatic buttress. In group 2 (n = 10), patients were treated with Class III elastics extending from infrazygomatic miniplates in the maxilla to symphyseal miniplates in the mandible. Group 3 (n = 10) was an untreated control group. The decision to discontinue orthopedic treatment was made when the patients had 3 to 4 mm of positive anterior overjet. Pretreatment, posttreatment, and observation 3-dimensional digital models were analyzed, superimposed, 3 dimensionally mapped, and sectioned. RESULTS: In this study, there were no significant changes in maxillary arch depth and maxillary or mandibular intermolar width before and after maxillary protraction or after the observation period in the control group. The mandibular arch depth decreased by a small but statistically significant amount only in groups 1 and 3. Superimposition of the pretreatment and posttreatment or observation maxillary 3-dimensional digital models showed minimal clinically significant dentoalveolar changes. CONCLUSIONS: Miniplate-anchored maxillary protraction protocols can accomplish maxillary advancement by eliminating movements of teeth and dentoalveolar changes. No spontaneous improvement in transverse deficiency was detected after correction of the anteroposterior deficiency at this age. Consequently, patients with transverse maxillary deficiency should have rapid maxillary expansion before or during the miniplate-anchored protraction period to improve the transverse deficiency.


Asunto(s)
Maloclusión de Angle Clase III/terapia , Técnica de Expansión Palatina/instrumentación , Placas Óseas , Cefalometría , Niño , Femenino , Humanos , Imagenología Tridimensional , Masculino , Métodos de Anclaje en Ortodoncia , Reproducibilidad de los Resultados , Resultado del Tratamiento
17.
Int J Biol Macromol ; 102: 272-283, 2017 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-28392380

RESUMEN

Cobalt ferrite nanoparticles (CF), titanate nanotubes (T), alginate (G) and their nanocomposite microparticles (CF/G and T/G) were prepared and used for efficient removal of Cu2+, Fe3+ and As3+ ions from water. The nanocomposites were characterized using field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), high resolution transmission electron microscopy (HRTEM), FTIR and vibrating sample magnetometer (VSM). In addition, the effects of pH, contact time, adsorbent weight and heavy metal ion concentration on the removal efficiency were investigated. Our results revealed a successful preparation of the nanocomposite particles. The optimized batch experiment conditions were found to be pH of 6.5, contact time of 2h and adsorbent weight of 0.15g. The removal efficiencies for Cu2+ using G, CF, T, CF/G and T/G were found to be 91%, 100%, 99.9%, 95% and 98%, respectively. While that of Fe3+ removal was 60%, 100%, 100%, 60% and 82%, respectively. Efficient removal of As3+ ions was also attained (98% upon using T nanoadsorbents). The current study demonstrated that the developed nanomaterials (CF and T) and their corresponding alginate-based nanocomposite microparticles could be further tailored and used as efficient adsorbents for the uptake of different heavy metal ions from wastewater.


Asunto(s)
Alginatos/química , Metales Pesados/química , Metales Pesados/aislamiento & purificación , Nanocompuestos/química , Contaminantes Químicos del Agua/química , Contaminantes Químicos del Agua/aislamiento & purificación , Adsorción , Cobalto/química , Compuestos Férricos/química , Ácido Glucurónico/química , Ácidos Hexurónicos/química , Concentración de Iones de Hidrógeno , Nanotubos/química , Titanio/química , Agua/química
18.
Am J Orthod Dentofacial Orthop ; 150(5): 751-762, 2016 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-27871701

RESUMEN

INTRODUCTION: The aim of this study was to evaluate and compare the effects of 2 protocols of bone-anchored maxillary protraction with an untreated control group. METHODS: Thirty growing Class III subjects with maxillary deficiency in the late mixed or early permanent dentition were included in the study. In group 1 (n = 10), skeletally anchored facemasks were used with miniplates placed at the zygomatic buttress. In group 2 (n = 10), the patients were treated with Class III elastics extending from infrazygomatic miniplates in the maxilla to symphyseal miniplates in the mandible. Group 3 (n = 10) was an untreated control group. Pretreatment and posttreatment cephalometric radiographs were analyzed. RESULTS: The treatment periods were 8 and 8.9 months in groups 1 and 2, respectively, and the untreated control group was observed for 9.4 months. The maxilla moved forward significantly in groups 1 and 2 compared with the untreated control group (4.87 mm in group 1, 5.81 mm in group 2); overjet and maxillary incisor display were improved without proclination or mesialization of the maxillary teeth relative to the maxillary base. Soft tissue harmony demonstrated the great improvement. However, group 1 showed more opening rotation of the mandible and lingual inclination of the mandibular incisors than did group 2. CONCLUSIONS: The 2 skeletal anchorage protocols for maxillary protraction effectively resolved the severe maxillary deficiently in growing Class III patients. However, vertical changes and retroclination of the mandibular incisors were better controlled by Class III elastics extending from the infrazygomatic miniplates in the maxilla to the symphyseal miniplates in the mandible (group 2).


Asunto(s)
Maloclusión de Angle Clase III/terapia , Métodos de Anclaje en Ortodoncia/métodos , Técnica de Expansión Palatina , Cefalometría , Niño , Femenino , Humanos , Masculino , Maloclusión de Angle Clase III/diagnóstico por imagen , Métodos de Anclaje en Ortodoncia/instrumentación , Radiografía Dental , Cigoma
19.
Life Sci ; 163: 1-10, 2016 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-27575704

RESUMEN

AIMS: Cyclosporine (CSA) elevates blood pressure (BP) and alters arterial baroreflex sensitivity (BRS) and vasoreactivity. In this study we determined whether the renin-angiotensin system (RAS) interplays with other vasopressor pathways in mediating the CSA actions. MATERIALS AND METHODS: Whole animal and isolated vascular preparations were employed to determine the effects of pharmacologic interruption of angiotensin II (Ang II), endothelin (ET), or thromboxane (TXA2) signaling on the adverse cardiovascular effects of CSA. KEY FINDINGS: CSA (25mg/kg/day i.p. for 7days) caused significant increases in BP that were paralleled with (i) reduced BRS measured by phenylephrine (BRSPE) or sodium nitroprusside (BRSSNP), (ii) enhanced aortic contractile responses to Ang II and U-46619 (thromboxane analogue), and (iii) reduced aortic eNOS expression and acetylcholine, but not SNP, vasorelaxations. Except for the reduced BRSSNP, the CSA effects disappeared upon concurrent administration of losartan (angiotensin AT1 receptor antagonist), captopril (angiotensin converting enzyme inhibitor), or their combination. Moreover, CSA augmentation of Ang II contractions was abolished after cyclooxygenase inhibition (indomethacin) or endothelin ETA/ETB receptor blockade (atrasentan/BQ788). By contrast, the blockade of thromboxane receptors (terutroban) failed to alter the CSA-evoked facilitation of Ang II responsiveness. SIGNIFICANCE: The facilitation of baroreflex control and inhibition of vascular responsiveness to Ang II and thromboxane contribute to the BP lowering effect of RAS inhibitors in CSA-treated rats. Further, endothelin receptors and vasoconstrictor prostanoids contribute to the CSA-evoked exaggeration of Ang II vascular responsiveness and hypertension.


Asunto(s)
Barorreflejo/efectos de los fármacos , Ciclosporina/farmacología , Hipertensión/fisiopatología , Sistema Renina-Angiotensina/fisiología , Vasoconstricción/efectos de los fármacos , Vasodilatación/efectos de los fármacos , Ácido 15-Hidroxi-11 alfa,9 alfa-(epoximetano)prosta-5,13-dienoico/farmacología , Acetilcolina/farmacología , Angiotensina II/farmacología , Animales , Aorta/efectos de los fármacos , Aorta/metabolismo , Atrasentán , Presión Sanguínea/efectos de los fármacos , Captopril/farmacología , Ciclosporina/antagonistas & inhibidores , Hipertensión/inducido químicamente , Indometacina/farmacología , Losartán/farmacología , Masculino , Naftalenos/farmacología , Óxido Nítrico Sintasa de Tipo III/biosíntesis , Nitroprusiato/farmacología , Oligopéptidos/farmacología , Fenilefrina/farmacología , Piperidinas/farmacología , Propionatos/farmacología , Pirrolidinas/farmacología , Ratas , Sistema Renina-Angiotensina/efectos de los fármacos
20.
Int J Hepatol ; 2015: 617623, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26640716

RESUMEN

Background & Aims. The currently available treatment for chronic hepatitis C (CHC) in children is costly and with much toxicity. So, predicting the likelihood of response before starting therapy is important. Methods. Serum adiponectin, vitamin D, and alpha-fetoprotein (AFP) were measured before starting pegylated-interferon/ribavirin therapy for 50 children with CHC. Another 21 healthy children were recruited as controls. Results. Serum adiponectin, vitamin D, and AFP were higher in the CHC group than healthy controls (p < 0.0001, p = 0.071, and p = 0.87, resp.). In univariate analysis, serum adiponectin was significantly higher in responders than nonresponders (p < 0.0001) and at a cutoff value ≥8.04 ng/mL it can predict treatment response by 77.8% sensitivity and 92.9% specificity, while both AFP and viremia were significantly lower in responders than nonresponders, p < 0.0001 and p = 0.0003, respectively, and at cutoff values ≤3.265 ng/mL and ≤235,384 IU/mL, respectively, they can predict treatment response with a sensitivity of 83.3% for both and specificity of 85.7% and 78.6%, respectively. In multivariate analysis, adiponectin was found to be the only independent predictor of treatment response (p = 0.044). Conclusions. The pretreatment serum level of adiponectin can predict the likelihood of treatment response, thus avoiding toxicities for those unlikely to respond to therapy.

SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...