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1.
Adv Exp Med Biol ; 1232: 77-83, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31893397

RESUMEN

Instantaneous orthostatic hypotension (INOH) is one of the main types of orthostatic dysregulation in children and adolescents. In patients with INOH arterial pressure drops considerably after active standing and is slow to recover. We investigated changes in cerebral oxygenation in the bilateral prefrontal cortex during an active standing test in juvenile INOH patients to evaluate changes in cerebral oxygen metabolism. We enrolled 82 INOH patients (mean age 13.8 ± 2.2 years, 52 mild and 30 severe patients) at Nihon University Itabashi Hospital from October 2013 to April 2018. We measured cerebral oxygenated hemoglobin, deoxygenated hemoglobin, and total hemoglobin levels in the bilateral prefrontal cortex using near-infrared spectroscopy during an active standing test. In severe INOH patients, cerebral oxygenation of the right prefrontal cortex remained constant when blood pressure dropped; however, de-oxy-Hb significantly increased. These findings confirm that there is asymmetrical autoregulation between the right and left prefrontal cortex.


Asunto(s)
Circulación Cerebrovascular , Hipotensión Ortostática , Adolescente , Circulación Cerebrovascular/fisiología , Niño , Homeostasis , Humanos , Hipotensión Ortostática/fisiopatología , Oxihemoglobinas , Espectroscopía Infrarroja Corta
2.
Cureus ; 11(9): e5608, 2019 Sep 09.
Artículo en Inglés | MEDLINE | ID: mdl-31700721

RESUMEN

So far, little attention has been paid to the increase in acute phase reactants (APRs) in patients with scurvy. We report that elevated levels of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and serum amyloid A were shown in a pediatric patient with scurvy despite the absence of inflammation. These peculiar findings are important to discriminate scurvy from other rheumatic diseases.

3.
Brain Dev ; 41(6): 559-562, 2019 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-30772096

RESUMEN

BACKGROUND: Confusional migraine is a rare type of migraine presenting as an acute confusional state. However, the mechanism of this confusional state remains unclear. SUBJECT AND METHODS: We examined an 11-year-old girl with confusional migraine, using electroencephalography, brain magnetic resonance imaging, cerebrovascular magnetic resonance angiography, and single-photon emission computed tomography to investigate cerebral blood flow changes. RESULTS: Our findings revealed vessel narrowing in the left middle and posterior cerebral artery territory, indicating vasospasm and suggesting that the confusion was caused by hypoperfusion. However, abnormal increased cerebral blood flow in the left middle and posterior cerebral artery territory was observed during the non-confusional state. CONCLUSION: The recorded cerebral blood flow changes are similar to those associated with migraine attacks, gradually changing from abnormally low to abnormally high during the confusional and post-confusional state.


Asunto(s)
Circulación Cerebrovascular/fisiología , Trastornos Migrañosos/fisiopatología , Niño , Confusión/fisiopatología , Electroencefalografía/métodos , Femenino , Humanos , Angiografía por Resonancia Magnética/métodos , Imagen por Resonancia Magnética/métodos , Trastornos Migrañosos/sangre , Trastornos Migrañosos/complicaciones , Tomografía Computarizada de Emisión de Fotón Único/métodos
4.
Naunyn Schmiedebergs Arch Pharmacol ; 387(4): 377-88, 2014 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-24322587

RESUMEN

To elucidate the roles played by the interstitial cells of Cajal in the myenteric layer (ICC-MY) in cholinergic neuromuscular transmission, we recorded mechanical and electrical activities in response to electrical field stimulation (EFS) of the ileal longitudinal muscle strips from WBB6F1-W/W(V) (W/W(V)) mutant mice, that lacked ICC-MY and compared with those in WBB6F1-+/+ (+/+) control mice. In +/+ muscle strips, EFS induced phasic contractions, which were abolished or strongly attenuated by atropine or tetrodotoxin. In W/W(V) preparations, EFS induced similar phasic contractions, but the cholinergic component was smaller than that in +/+ strips. This was despite of the fact that the contractions because of exogenous applications of carbachol and high K(+) solution in W/W(V) strips were comparable to or rather greater than those in the +/+ preparations. EFS induced atropine-sensitive excitatory junction potentials (EJPs) in the +/+ longitudinal smooth muscle cells but not in W/W(V) cells. In the presence of eserine, EFS induced atropine-sensitive EJPs in W/W(V) cells. These results suggest that ICC-MY mediate the cholinergic neuromuscular transmission in mouse ileal longitudinal smooth muscles. In addition, the other pathway in which ICC-MY are not involved can operate concomitantly.


Asunto(s)
Íleon/fisiología , Células Intersticiales de Cajal/fisiología , Músculo Liso/fisiología , Plexo Mientérico/fisiología , Adrenérgicos/farmacología , Animales , Atropina/farmacología , Colinérgicos/farmacología , Estimulación Eléctrica , Guanetidina/farmacología , Íleon/inervación , Técnicas In Vitro , Masculino , Potenciales de la Membrana , Ratones , Ratones Mutantes , Contracción Muscular , Músculo Liso/inervación , NG-Nitroarginina Metil Éster/farmacología , Unión Neuromuscular/fisiología , Fisostigmina/farmacología , Transmisión Sináptica
5.
Proc Natl Acad Sci U S A ; 108(42): 17533-7, 2011 Oct 18.
Artículo en Inglés | MEDLINE | ID: mdl-21972415

RESUMEN

A lipid extract of Perna canaliculus (New Zealand green-lipped mussel) has reportedly displayed anti-inflammatory effects in animal models and in human controlled studies. However, the anti-inflammatory lipid components have not been investigated in detail due to the instability of the lipid extract, which has made the identification of the distinct active components a formidable task. Considering the instability of the active component, we carefully fractionated a lipid extract of Perna canaliculus (Lyprinol) and detected furan fatty acids (F-acids). These naturally but rarely detected fatty acids show potent radical-scavenging ability and are essential constituents of plants and algae. Based on these data, it has been proposed that F-acids could be potential antioxidants, which may contribute to the protective properties of fish and fish oil diets against chronic inflammatory diseases. However, to date, in vivo data to support the hypothesis have not been obtained, presumably due to the limited availability of F-acids. To confirm the in vivo anti-inflammatory effect of F-acids in comparison with that of eicosapentaenoic acid (EPA), we developed a semisynthetic preparation and examined its anti-inflammatory activity in a rat model of adjuvant-induced arthritis. Indeed, the F-acid ethyl ester exhibited more potent anti-inflammatory activity than that of the EPA ethyl ester. We report on the in vivo activity of F-acids, confirming that the lipid extract of the green-lipped mussel includes an unstable fatty acid that is more effective than EPA.


Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Ácidos Grasos/farmacología , Perna/química , Animales , Antiinflamatorios no Esteroideos/química , Antiinflamatorios no Esteroideos/aislamiento & purificación , Artritis Experimental/tratamiento farmacológico , Ácidos Grasos/química , Ácidos Grasos/aislamiento & purificación , Femenino , Furanos/química , Furanos/aislamiento & purificación , Furanos/farmacología , Humanos , Lípidos/química , Masculino , Estructura Molecular , Oncorhynchus keta/metabolismo , Ratas , Ratas Endogámicas Lew , Ratas Wistar , Testículo/química
6.
Toxicol In Vitro ; 24(6): 1720-6, 2010 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-20510349

RESUMEN

Many effects of sesamol, an antioxidative constituent of sesame oil, have been reported for human health benefits due to its antioxidative action. However, we recently isolated two cytotoxic products, trimer and tetramer of sesamol, from oxidation of sesamol by an assay-guided purification. In this study, we have revealed some cytotoxic characteristics of these products in rat thymocytes and human leukemia K562 cells. Incubation of cells with trimer or tetramer at 10-30 microM for 24h significantly increased cell lethality and population of rat thymocytes containing hypodiploid DNA, suggesting cell death with DNA fragmentation, while it was not the case for 30 microM sesamol. The cytotoxic action of tetramer was more potent than that of trimer in rat thymocytes when their concentrations were 10-30 microM. The incubation of cells with 10 microM tetramer for 24h increased the population of cells with exposed phosphatidylserine, the activity of caspases, and the nick of DNA. These results indicate tetramer-induced apoptosis. In K562 cells, the incubation with tetramer at 10 microM for 72 h significantly inhibited the growth without affecting the lethality. However, tetramer at 30 microM significantly increased cell lethality. It is likely that tetramer exerts more cytotoxic action on normal non-proliferative cells (rat thymocytes) rather than proliferative cancer cells (human leukemia K562 cells). It may be necessary to consider the condition for preservation of sesamol and the safety of products from in vivo oxidation of sesamol for human health.


Asunto(s)
Antioxidantes/toxicidad , Apoptosis/efectos de los fármacos , Benzodioxoles/toxicidad , Linfocitos/efectos de los fármacos , Fenoles/toxicidad , Aceite de Sésamo/química , Animales , Antioxidantes/química , Benzodioxoles/química , Supervivencia Celular/efectos de los fármacos , Fraccionamiento Químico , Humanos , Células K562 , Linfocitos/patología , Oxidación-Reducción , Fenoles/química , Ratas , Ratas Wistar , Timo/citología
7.
Masui ; 58(7): 913-6, 2009 Jul.
Artículo en Japonés | MEDLINE | ID: mdl-19618836

RESUMEN

The low femoral approach for catheterization of the central venous line is a difficult procedure because the surface landmark technique is not helpful. We report a case of patient who required the distal femoral cannulation. Re-catheter cannulation was planned for the patient after the major cardiovascular surgery. The right internal jugular vein showed the venous thrombus attributed to the previous catheter placement for the operation. The left internal, jugular vein had anatomical anomaly at the level of cricoid cartilage and the last approach for cannulation was not feasible. The right subclavian and right femoral regions were scarred with the surgical procedure. Finally, we performed the distal femoral cannulation using real time ultrasound guidance. When multiple difficulties for venous access are present, ultrasound-guided low approach of femoral vein might be one of the solutions.


Asunto(s)
Cateterismo Venoso Central/métodos , Vena Femoral , Anciano de 80 o más Años , Aneurisma Disecante/cirugía , Aneurisma de la Aorta Torácica/cirugía , Procedimientos Quirúrgicos Cardiovasculares , Vena Femoral/diagnóstico por imagen , Humanos , Masculino , Ultrasonografía Intervencional/métodos
8.
Nanotechnology ; 17(22): 5539-43, 2006 Nov 28.
Artículo en Inglés | MEDLINE | ID: mdl-21727321

RESUMEN

Magnetic nanoparticle arrays have been fabricated by combining chemically synthesized Fe(3)O(4) nanoparticles with a diblock copolymer template substrate consisting of self-assembled polystyrene (PS) dots in a polymethylmethacrylate (PMMA) matrix. The influence of the volume fraction of the Fe(3)O(4) suspending solution and the withdrawal speed of the template on the formation of array structures was investigated. A small volume fraction of the nanoparticles and low withdrawal speed play an important role in the fabrication of the patterned arrays of nanoparticles via template assisted self-assembly. Below a withdrawal speed of 0.5 mm s(-1) and a nanoparticle volume fraction below 0.05 vol% (in particular, at extremely high dilutions of less than 0.01 vol%), the selective deposition of one to several nanoparticles on every single PS dot becomes possible.

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