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1.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(2): 515-519, 2021 Apr.
Artículo en Chino | MEDLINE | ID: mdl-33812423

RESUMEN

OBJECTIVE: To study the effect of PX-12 on apoptosis of multiple myeloma (MM) cell line induced by bortezomib. METHODS: MM cell line H929 cells were divided into PX-12 group, bortezomib group, combination group, and control group. 5.0 µmol/L PX-12, 20 nmol/L bortezomib, combination of the two drugs, and DMSO were given to the above mentioned group, respectively. After culture for 24, 48, and 72 hours, the changes of cell viability were observed, the MM cell activity was detected by MTT method, and the cell cycle distribution and apoptosis of each group was detected by flow cytometry. The intracellular ROS level was measured by H2DCFDA probe labeling. RESULTS: MTT assay showed that after culture for 72 hours, the activity of H929 cells in PX-12 group (P<0.05) and bortezomib group (P<0.01) was significantly lower than that in the control group, while that in the combination group was decreased most significantly (P<0.01). After culture for 48 hours, cells in G1 phase in PX-12 group was decreased to 40%, while cells in S phase and G2/M phase was increased to 28% and 40%, respectively. The cells in bortezomib group also showed a similar distribution after being treated. After treated with PX-12 and bortezomib, the cells in G1 phase were decreased significantly to 19% and 12% in S phase, but increased significantly to 68% in G2/M phase, which was significantly different from PX-12 group and bortezomib group (P<0.01). After culture for 72 hours, the apoptosis rate was 71.3% in the combination group, which was significantly higher than that in PX-12 group, bortezomib group, and control group (20.6%, 33.3%, 10.6%)(P<0.01). After culture for 24 hours, the intracellular ROS level in the combination group was 12015±430.2, which was higher than that in the PX-12 group, bortezomib group, and control group (6729±352.8, 2651±228.3, 1098±164.6, respectively) (P<0.01). CONCLUSION: PX-12 can increase the apoptosis of MM cell line H929 induced by bortezomib, which may be caused by increasing of ROS level.


Asunto(s)
Mieloma Múltiple , Apoptosis , Bortezomib/farmacología , Línea Celular Tumoral , Proliferación Celular , Humanos
2.
Med Image Anal ; 71: 102035, 2021 Mar 19.
Artículo en Inglés | MEDLINE | ID: mdl-33813286

RESUMEN

The loss function is an important component in deep learning-based segmentation methods. Over the past five years, many loss functions have been proposed for various segmentation tasks. However, a systematic study of the utility of these loss functions is missing. In this paper, we present a comprehensive review of segmentation loss functions in an organized manner. We also conduct the first large-scale analysis of 20 general loss functions on four typical 3D segmentation tasks involving six public datasets from 10+ medical centers. The results show that none of the losses can consistently achieve the best performance on the four segmentation tasks, but compound loss functions (e.g. Dice with TopK loss, focal loss, Hausdorff distance loss, and boundary loss) are the most robust losses. Our code and segmentation results are publicly available and can serve as a loss function benchmark. We hope this work will also provide insights on new loss function development for the community.

3.
Antioxidants (Basel) ; 10(3)2021 Mar 10.
Artículo en Inglés | MEDLINE | ID: mdl-33801821

RESUMEN

To expand the applications and enhance the stability and bioactivity of resveratrol (RE), and to simultaneously include the potential health benefits of short chain fatty acids (SCFA) esters of RE were prepared by Steglich reactions with acetic, propionic, and butyric acids, respectively. RE and the esterified RE-SCFA products (including RAE, RPE, and RBE) were analyzed using nuclear magnetic resonance (NMR), Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), differential thermal analysis (DTA), and liquid chromatography-mass spectrometry (LC-MS). The FTIR and 13C NMR spectra of the esterified products included ester-characteristic peaks at 1751 cm-1 and 171 ppm, respectively. Moreover, the peaks in the range of 1700 to 1600 cm-1 in the FTIR spectra of the esterified products indicated that the esterification of RE-SCFA was successful. The TGA results revealed that the RE-SCFA esters decomposed at lower temperatures than RE. The peaks in the LC-MS profiles of the esterified products indicated the formation of mono- and diesters, and the calculated monoester synthesis rates ranged between 45.81 and 49.64%. The RE esters inhibited the Cu2+-induced low-density lipoprotein oxidation reaction, exhibited antioxidant activity in bulk oil, and effectively inhibited the hydroxyl radical-induced DNA scission. Moreover, the RE-SCFA esters had better hydrogen peroxide scavenging activity than RE. Our results are the first in the literature to successfully including short chain fatty acids in the esters of resveratrol, and the products could be used as a functional food ingredient in processed foods or can be used as dietary supplements to promote health.

4.
Sensors (Basel) ; 21(7)2021 Mar 24.
Artículo en Inglés | MEDLINE | ID: mdl-33805211

RESUMEN

In this paper, a manipulation planning method for object re-orientation based on semantic segmentation keypoint detection is proposed for robot manipulator which is able to detect and re-orientate the randomly placed objects to a specified position and pose. There are two main parts: (1) 3D keypoint detection system; and (2) manipulation planning system for object re-orientation. In the 3D keypoint detection system, an RGB-D camera is used to obtain the information of the environment and can generate 3D keypoints of the target object as inputs to represent its corresponding position and pose. This process simplifies the 3D model representation so that the manipulation planning for object re-orientation can be executed in a category-level manner by adding various training data of the object in the training phase. In addition, 3D suction points in both the object's current and expected poses are also generated as the inputs of the next operation stage. During the next stage, Mask Region-Convolutional Neural Network (Mask R-CNN) algorithm is used for preliminary object detection and object image. The highest confidence index image is selected as the input of the semantic segmentation system in order to classify each pixel in the picture for the corresponding pack unit of the object. In addition, after using a convolutional neural network for semantic segmentation, the Conditional Random Fields (CRFs) method is used to perform several iterations to obtain a more accurate result of object recognition. When the target object is segmented into the pack units of image process, the center position of each pack unit can be obtained. Then, a normal vector of each pack unit's center points is generated by the depth image information and pose of the object, which can be obtained by connecting the center points of each pack unit. In the manipulation planning system for object re-orientation, the pose of the object and the normal vector of each pack unit are first converted into the working coordinate system of the robot manipulator. Then, according to the current and expected pose of the object, the spherical linear interpolation (Slerp) algorithm is used to generate a series of movements in the workspace for object re-orientation on the robot manipulator. In addition, the pose of the object is adjusted on the z-axis of the object's geodetic coordinate system based on the image features on the surface of the object, so that the pose of the placed object can approach the desired pose. Finally, a robot manipulator and a vacuum suction cup made by the laboratory are used to verify that the proposed system can indeed complete the planned task of object re-orientation.

5.
Life (Basel) ; 11(3)2021 Mar 05.
Artículo en Inglés | MEDLINE | ID: mdl-33807545

RESUMEN

Deep learning-based software is developed to assist physicians in terms of diagnosis; however, its clinical application is still under investigation. We integrated deep-learning-based software for diabetic retinopathy (DR) grading into the clinical workflow of an endocrinology department where endocrinologists grade for retinal images and evaluated the influence of its implementation. A total of 1432 images from 716 patients and 1400 images from 700 patients were collected before and after implementation, respectively. Using the grading by ophthalmologists as the reference standard, the sensitivity, specificity, and area under the receiver operating characteristic curve (AUC) to detect referable DR (RDR) were 0.91 (0.87-0.96), 0.90 (0.87-0.92), and 0.90 (0.87-0.93) at the image level; and 0.91 (0.81-0.97), 0.84 (0.80-0.87), and 0.87 (0.83-0.91) at the patient level. The monthly RDR rate dropped from 55.1% to 43.0% after implementation. The monthly percentage of finishing grading within the allotted time increased from 66.8% to 77.6%. There was a wide range of agreement values between the software and endocrinologists after implementation (kappa values of 0.17-0.65). In conclusion, we observed the clinical influence of deep-learning-based software on graders without the retinal subspecialty. However, the validation using images from local datasets is recommended before clinical implementation.

6.
Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi ; 35(4): 293-296;301, 2021 Apr.
Artículo en Chino | MEDLINE | ID: mdl-33794623

RESUMEN

Objective:The result consistency between the new remote radar monitoring equipment and PSG detection is compared. Methods:The monitoring results of 71 patients were randomly selected to observe and compare the difference of monitoring data of the same patient with two kinds of detection equipment. Results:Using AHI≥5 as the diagnostic criteria, compared with PSG monitoring results, the sensitivity, specificity and Youden index of the new remote radar monitoring equipment were 96.2%, 89.5% and 0.857(P<0.01) respectively. Compared with PSG monitoring results, when LSaO2≤90% in PSG monitoring, the sensitivity, specificity and Youden index of the new remote radar monitoring equipment were 94.3%, 88.9% and 0.832(P<0.01) respectively. Conclusion: There the new remote radar monitoring device can be used to monitor OSA.


Asunto(s)
Radar , Apnea Obstructiva del Sueño , Humanos , Polisomnografía , Sensibilidad y Especificidad , Apnea Obstructiva del Sueño/diagnóstico
7.
J Mol Cell Cardiol ; 2021 Apr 02.
Artículo en Inglés | MEDLINE | ID: mdl-33819456

RESUMEN

AIM: Pulmonary hypertension (PH) is a pathophysiological syndrome with functional abnormalities of the pulmonary artery and heart, eventually becoming life threatening to the patients. Autophagy-related gene 7 (ATG)-7 is involved in many cardiovascular diseases, but little is known about the specific role of ATG-7 in the development of PH. We aimed to examine the expression of ATG-7 in PH patients and PH mice, specifically investigate pulmonary physiological responses in a mouse model with conditional deletion of ATG-7 in smooth muscle cells (SMCs) and further clarify the mechanism of PH caused by ATG-7 deficiency. METHODS AND RESULTS: SMC-ATG-7-/- mice underwent echocardiography and subsequent pulmonary arterial pressure (PAP) checks. The PAP was lower in wild-type (WT) mice (22.6 ±â€¯2.0 mmHg) than knockout (KO) mice (34.0 ±â€¯2.5 mmHg; P < 0.001). Pulmonary artery resistance was increased in KO (17.61 ±â€¯2.03 mm2·s-1) versus WT mice (8.91 ±â€¯1.62 mm2·s-1; P < 0.005). Combined with these statistics, SMC-ATG7-/- mice were diagnosed with PH. The increase of ATG-7 expression in vessels from PH patients and PH mice were assessed and the effects of ATG-7 on vascular remodeling were investigated in SMCs using relevant methods. We also identified silencing ATG-7 in SMCs induced the increased level of Ca2+ and abnormal proliferation through PP2A/ 4EBP-1/ elf-4E pathway. CONCLUSIONS: ATG-7 affects vascular remodeling and exerts a protective function during the pathogenesis of PH. Our study revealed a novel mechanism ATG-7 deficiency promotes cell proliferation via the interaction between PP2A, 4EBP1 and elf-4E.

8.
Ann Thorac Surg ; 2021 Apr 02.
Artículo en Inglés | MEDLINE | ID: mdl-33819473

RESUMEN

BACKGROUND: The study aimed to compare the safety and effectiveness between fenestrated technique with or without chimney stent and single-branched stent-graft for isolated left subclavian artery (LSA) revascularization during endovascular repair of acute type B aortic dissection (ATBAD) with unfavorable proximal landing zone. METHODS: From January 2016 to December 2019, 65 ATBAD patients with unfavorable proximal landing zone were treated with either the fenestrated technique (n=34, group A) or single-branched stent-graft (n=31, group B). Type I endoleak, retrograde type A dissection, stroke, LSA patency, 30-day mortality, and aortic remodeling were systematically recorded and retrospectively analyzed. RESULTS: Technical success rates for groups A and group B were 94.12% and 100%, respectively. LSA primary patency was achieved for all enrolled patients. The incidence of type I endoleak, retrograde type A dissection, stroke, and 30-day mortality was 5.9%, 5.9%, 2.9%, and 2.9% in group A, respectively, but none were encountered in group B. LSA Occlusion was observed in three and two patients during a mean follow-up of 16.18±2.08 and 15.19±2.68 months in groups A and B, respectively. After the procedure, significant aortic remodeling was detected in both groups during follow-up. CONCLUSIONS: Both techniques are feasible and safe for isolated LSA revascularization during endovascular repair for ATBAD. Apart from the associated perioperative risks of complications and mortality for the fenestrated technique, both procedures contributed to favorable aortic remodeling.

9.
Mol Reprod Dev ; 2021 Apr 06.
Artículo en Inglés | MEDLINE | ID: mdl-33821543

RESUMEN

Intraflagellar transport 27 (IFT27) is a key regulator for spermiogenesis and male fertility in mice. ATP8a1, a protein involved in the translocation of phosphatidylserine and phosphatidylethanolamine across lipid bilayers, is the strongest binding partner of IFT27. To investigate the role of ATP8a1 in spermatogenesis and male fertility, the global Atp8a1 knockout mice were analyzed. All mutant mice were fertile, and sperm count and motility were comparable to the control mice. Examination of testis and epididymis by hematoxylin and eosin staining did not reveal major histologic defects. These observations demonstrate that ATP8a1 is not a major spermatogenesis regulator. Given that a tissue-specific paralogue of ATP8a1, ATP8a2, is present, further studies with double-knockout models are warranted to delineate any compensatory functions of the two proteins.

10.
Environ Pollut ; 281: 116994, 2021 Mar 29.
Artículo en Inglés | MEDLINE | ID: mdl-33819668

RESUMEN

The Fuyang River system (FRS) in north China, for a long time, is seriously polluted with organic compounds and heavy metals due to industrialization. However, the information on heavy metal pollution in this area is still limited, and health risks raised by trace elements are neglected up to now. To characterize the heavy metal pollutants and assess their potential ecological risks scientifically in FRS, surface sediments were collected from 66 sampling sites selected according to the hydrological and anthropogenic conditions along the river. A total of twelve metal pollutants (e.g., Cr, As, and Hg) in the sediments were detected among the distributaries. A combining application of geoaccumulation index (Igeo), ratio of secondary phase and primary phase (RSP), and the ecological risk factor (Eri) in this study gave systematic assessment results of single or combined pollution status raised by heavy metals in this area. The results show that Cr, Ni, Cu, As, Cd, Co, and Sn are mainly dispersed in the river reaches of Xingtai City and pose potential health risks in midstream, as per the geoaccumulation index and Pearson's correlation analyses. In particular, Cd accumulates strongly in sediments of Ming River and Aixinzhuang dam from Xingtai City. In upstream and downstream of FRS, the potential ecological risk is low, except in Yongnian County where high ecological risk was caused by Cd and Hg. These findings provide new insights into the pollution characteristics and assessment of the potential ecological risks induced by heavy metals along FRS, which suggest new directions should strategically tend to typical pollutants control by policy formulation and taking effective measures to prevent and manage heavy metal pollution in North China.

11.
Artículo en Inglés | MEDLINE | ID: mdl-33823584

RESUMEN

The continuous degradation of carbonate electrolytes and the dissolution of transition metal cations due to parasitic reactions on the cathode-electrolyte interphase (CEI) block the practical application of LiNi0.5Mn1.5O4-based lithium-ion batteries (LNMO-based LIBs) at a high voltage. cis-1,2,3,6-Tetrahydrophthalic anhydride (CTA) has been used as a functional additive in a carbonate baseline electrolyte (BE) for constructing the CEI film to enhance the cyclic stability of LNMO-based LIBs. The LNMO/Li cell with CTA exhibits a preponderant capacity retention of 83.3% compared with those of propionic anhydride (PA) (46.5%) and BE (13.6%) after 500 cycles at the current density of 1 C from 3.5 to 4.9 V. Additionally, the LNMO/graphite full cell with CTA still has a higher capacity retention of 95.46% even after 300 cycles at 1 C. By characterizations, it is reasonably demonstrated that CTA was oxidated to participate in the construction of a CEI film. An unsaturated aromatic group was introduced into the composition of the CEI film along with CTA in the formation process of the CEI film, which further improved the antioxidative activity of the CEI film under the influence of field-effect. Specifically, the CEI film obtains appreciable stability because of its higher antioxidative activity under the influence of field-effect. The stabilized CEI can significantly suppress the parasitic reactions of electrolytes, decrease the consumption of active-Li+, and protect the LNMO cathode structure, thereby enhancing the cyclic compatibility of LNMO-based LIBs with the carbonate electrolytes from 3.5 to 4.9 V.

12.
J Tradit Chin Med ; 41(2): 227-235, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33825402

RESUMEN

OBJECTIVE: To evaluate the efficacy of Liuwei Dihuang formula ( LWDHF) on endothelial cells, and to study the mechanism behind the action of modulating expression of estrogen receptors. METHODS: Hydrogen peroxide (H2O2) was applied to induce the apoptosis of human umbilical vein endothelial cells (HUVECs). The concentration of nitric oxide (NO), endothelial nitric oxide synthase (eNOS) and inducible NOS (iNOS) were measured by assay kits. Western blot and real-time polymerase chain reaction (RT-PCR) were used to detect the expression of iNOS, eNOS, b-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), estrogen receptor (ER) α and ERß. Also, small interfering RNA (siRNA) was involved to confirm whether the protective effects of LWDHF was medicated by ERs. In vivo, the female rats were ovariectomized to establish postmenopausal vascular injury model. Then the model rats were divided into three groups and treated with saline, estradiol and LWDHF respectively. The concentration of NO and NOS in serum were measured by assay kits, and the expression of Bax, Bcl-2, ERα and ERß were detected by western blot and immunohistochemistry. RESULTS: In vitro study, LWDHF significantly protected HUVECs from H2O2-induced apoptosis, with the increase of Bcl-2 and the decrease of Bax. The treatment with LWDHF inhibited concentration of NO and iNOS, and upregulated the expression of eNOS, ERα and ERß. In addition, ERα siRNA could block the protective effects of LWDHF, while ERß siRNA showed little influence. In vivo, the treatment with LWDHF suppressed the vascular injury and reduced the level of NO and NOS. LWDHF increased the expression of Bcl-2, ERα and ERß, as well as inhibiting the Bax expression. CONCLUSION: LWDHF could improve endothelial function and protect HUVECs from apoptosis via increasing the expression of ERα.

13.
Eur J Neurosci ; 2021 Apr 05.
Artículo en Inglés | MEDLINE | ID: mdl-33818849

RESUMEN

Since the depressive disorder manifests complex and diverse symptoms clinically, its pathological mechanism and therapeutic options are difficult to determine. In recent years, the advent of optogenetics, chemogenetics and viral tracing techniques, along with the well-established rodent model of depression, has led to a shift in the focus of depression research from single molecules to neural circuits. In virtue of the powerful tools above, psychiatric disorder such as depression could be well related to the disfunction of brain's connection. Moreover, compelling studies also support that the diversity of depressive behaviour could be involved with the discrete changes in a distinct circuit of the brain. Therefore, summarising the differential changes of the neural circuits in mice with depression-like behaviour may provide a better understanding of the causal relationships between neural circuit and depressive behaviour. Here, we focus on the changes in the neural circuitry underlying various depression-like phenotypes, including motivation, despair, social avoidance and comorbid sequelae, which may provide an explanation to circuit-specific discrepancy in depression-like behaviour.

14.
J Diabetes Res ; 2021: 5398645, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33791389

RESUMEN

Objective: Accumulating evidence suggests the critical role of autophagy in the pathogenesis of diabetic retinopathy (DR). In the current study, we aim to identify autophagy genes involved in DR via microarray analyses. Methods: Gene microarrays were performed to identify differentially expressed lncRNAs/mRNAs between normal and DR retinas. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses of lncRNA-coexpressed mRNAs were used to determine the related pathological pathways and biological modules. Real-time polymerase chain reactions (PCR) were conducted to validate the microarray analyses. Results: A total of 2474 significantly dysregulated lncRNAs and 959 differentially expressed mRNAs were identified in the retina of DR. Based upon Signalnet analysis, Bcl2, Gabarapl2, Atg4c, and Atg16L1 participated the process of cell death in DR. Moreover, real-time PCR revealed significant upregulation of Atg16L1. Conclusion: This study indicated the importance and potential role of Atg16L1, one of the autophagy genes, as a biomarker in DR development and progression.

15.
Cancer Sci ; 2021 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-33793038

RESUMEN

Levothyroxine is a widely prescribed medication for the treatment of an underactive thyroid. The relationship between levothyroxine use and cancer risk is largely underdetermined. To investigate the magnitude of the possible association between levothyroxine use and cancer risk, this retrospective case-control study was conducted using Taiwan's Health and Welfare Data Science Center (HWDC) database. Cases were defined as all patients who were aged ≥20 years and had a first-time diagnosis of any site cancer for the period between 2001 and 2011. Multivariable conditional logistic regression models were used to calculate an adjusted odds ratio (AORs) to reduce potential confounding factors. A total of 601,733 cases and 2,406,932 controls were included in this current study. Levothyroxine users showed a 50% higher risk of any site cancer (AOR: 1.50, 95% CI: 1.46-1.54; p<0.0001) compared with non-users. Additionally, significant increased risks were also observed in brain cancer (AOR: 1.90, 95% CI: 1.48-2.44; p<0.0001), skin cancer (AOR: 1.42, 95% CI: 1.17-1.72; p<0.0001), pancreatic cancer (AOR: 1.27, 95% CI: 1.01-1.60; p=0.03), and female breast cancer (AOR: 1.24, 95% CI: 1.15-1.33; p<0.0001). Our study results showed that levothyroxine use was significantly associated with an increased risk of cancer, particularly brain, skin, pancreatic, and female breast cancers. Since levothyroxine still remains a highly effective therapy for hypothyroidism; therefore, physicians should carefully consider levothyroxine therapy, and monitor patients' condition timely for avoiding the unexpected consequence. Additional studies are needed to confirm these findings and to evaluate the potential biological mechanism.

16.
Nat Commun ; 12(1): 1383, 2021 03 02.
Artículo en Inglés | MEDLINE | ID: mdl-33654063

RESUMEN

In this study, we investigate the seroprevalence of SARS-CoV-2 antibodies among blood donors in the cities of Wuhan, Shenzhen, and Shijiazhuang in China. From January to April 2020, 38,144 healthy blood donors in the three cities were tested for total antibody against SARS-CoV-2 followed by pseudotype SARS-CoV-2 neutralization tests, IgG, and IgM antibody testing. Finally, a total of 398 donors were confirmed positive. The age- and sex-standardized SARS-CoV-2 seroprevalence among 18-60 year-old adults (18-65 year-old in Shenzhen) was 2.66% (95% CI: 2.24%-3.07%) in Wuhan, 0.033% (95% CI: 0.0029%-0.267%) in Shenzhen, and 0.0028% (95% CI: 0.0001%-0.158%) in Shijiazhuang, respectively. Female sex and older-age were identified to be independent risk factors for SARS-CoV-2 seropositivity among blood donors in Wuhan. As most of the population of China remained uninfected during the early wave of the COVID-19 pandemic, effective public health measures are still certainly required to block viral spread before a vaccine is widely available.


Asunto(s)
/patogenicidad , Anticuerpos Antivirales/sangre , Donantes de Sangre/estadística & datos numéricos , /epidemiología , China/epidemiología , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Pruebas de Neutralización , Prevalencia , Factores de Riesgo , /inmunología
17.
Water Environ Res ; 2021 Mar 03.
Artículo en Inglés | MEDLINE | ID: mdl-33657245

RESUMEN

Fe(II) is one of the commonly used additives in wastewater treatment and proved to be beneficial for promoting microbial activity. In this study, the effects of Fe(II) on the specific anammox activity (SAA) and reactor performance were proved to be concentration-dependent. In the short-term experiment, low concentration of Fe(II) (5-80 mg/L) significantly enhanced the SAA, while high concentration of Fe(II) (120-300 mg/L) inhibited the SAA. It was confirmed that anammox can be domesticated after long-term exposure to low Fe(II) concentration, and the SAA could be further enhanced by higher Fe(II) concentration in the following phases. In addition, as an important factor for anammox granulation and maintaining the SAA, the extracellular polymeric substance (EPS) was also affected by Fe(II) addition. In spite of the effects on SAA and EPS, Fe(II) was proved to be the key factor that enhances the N2 O emission via abiotic pathway in the anammox reactor. PRACTITIONER POINTS: Low Fe(II) concentrations enhanced SAA, while high concentrations inhibited SAA. Long-term acclimatization by Fe(II) improved the tolerance of anammox to Fe(II). Fe(II) affects the amount and constituent of EPS and the performance of anammox granules. Accumulation of Fe(II) in the AAFEB reactor promoted the N2 O emission.

18.
PLoS Negl Trop Dis ; 15(3): e0009274, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33780454

RESUMEN

In 2015, China and other member states of the United Nations adopted the goal of eliminating dog-mediated rabies by 2030. China has made substantial progress in reducing dog-mediated human rabies since peaking with more than 3,300 reported cases in 2007. To further improve coordination and planning, the Chinese Center for Disease Control and Prevention, in collaboration with the United States Centers for Disease Control and Prevention, conducted a Stepwise Approach towards Rabies Elimination (SARE) assessment in March 2019. Assessment goals included outlining progress and identifying activities critical for eliminating dog-mediated rabies. Participants representing national, provincial and local human and animal health sectors in China used the SARE assessment tool to answer 115 questions about the current dog-mediated rabies control and prevention programs in China. The established surveillance system for human rabies cases and availability of post-exposure prophylaxis were identified as strengths. Low dog vaccination coverage and limited laboratory confirmation of rabid dogs were identified gaps, resulting in an overall score of 1.5 on a scale of 0 to 5. Participants outlined steps to increase cross-sectoral information sharing, improve surveillance for dog rabies, increase dog vaccination coverage, and increase laboratory capacity to diagnose rabies at the provincial level. All assessment participants committed to strengthening cross-sector collaboration using a One Health approach to achieve dog-mediated human rabies elimination by 2030.

19.
J Colloid Interface Sci ; 594: 531-539, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33774409

RESUMEN

Herein we develop a novel and effective alkoxide hydrolysis approach to in-situ construct the trimanganese tetraoxide (Mn3O4)/graphene nanostructured composite as high-performance anode material for lithium-ion batteries (LIBs). This is the first report on the synthesis of Mn3O4/graphene composite via a facile hydrolysis of the manganese alkoxide (Mn-alkoxide)/graphene precursor. Before hydrolysis, two dimensional (2D) Mn-alkoxide nanoplates are closely adhered to 2D graphene nanosheets via Mn-O chemical bonding. After hydrolysis, the Mn-alkoxide in-situ converts to Mn3O4, while the Mn-O bond is preserved. This leads to a robust Mn3O4/graphene hybrid architecture with 15 nm Mn3O4 nanocrystals homogeneously anchoring on graphene nanosheets. This not only prevents the Mn3O4 nanocrystals agglomeration but also inversely mitigates the graphene nanosheets restacking. Moreover, the flexible and conductive graphene nanosheets can accommodate the volume change. This maintains the structural and electrical integrity of the Mn3O4/graphene electrode during the cycling process. As a result, the Mn3O4/graphene composite displays superior lithium storage performance with high reversible capacity (741 mAh g-1 at 100 mA g-1), excellent rate capability (403 mAh g-1 at 1000 mA g-1) and long cycle life (527 mAg g-1 after 300 cycles at 500 mA g-1). The electrochemical performance highlights the importance of rational design nanocrystals anchoring on graphene nanosheets for high-performance LIBs application.

20.
Bioorg Chem ; 110: 104813, 2021 Mar 10.
Artículo en Inglés | MEDLINE | ID: mdl-33774493

RESUMEN

MutT Homolog 1 (MTH1) has been proven to hydrolyze oxidized nucleotide triphosphates during DNA repair. It can prevent the incorporation of wrong nucleotides during DNA replication and mitigate cell apoptosis. In a cancer cell, abundant reactive oxygen species can lead to substantial DNA damage and DNA mutations by base-pairing mismatch. MTH1 could eliminate oxidized dNTP and prevent cancer cells from entering cell death. Therefore, inhibition of MTH1 activity is considered to be an anti-cancer therapeutic target. In this study, high-throughput screening techniques were combined with a fragment-based library containing 2,313 compounds, which were used to screen for lead compounds with MTH1 inhibitor activity. Four compounds with MTH1 inhibitor ability were selected, and compound MI0639 was found to have the highest effective inhibition. To discover the selectivity and specificity of this action, several derivatives based on the MTH1 and MI0639 complex structure were synthesized. We compared 14 complex structures of MTH1 and the various compounds in combination with enzymatic inhibition and thermodynamic analysis. Nanomolar-range IC50 inhibition abilities by enzyme kinetics and Kd values by thermodynamic analysis were obtained for two compounds, named MI1020 and MI1024. Based on structural information and compound optimization, we aim to provide a strategy for the development of MTH1 inhibitors with high selectivity and specificity.

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