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1.
Food Chem ; 334: 127560, 2021 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-32711271

RESUMEN

Post-fermented Pu-erh tea (PFPT) is a microbially-fermented tea with distinct sensory qualities and multiple health benefits. Aspergillus are the dominant fungi in the fermentation and the main contributors to the characteristics of PFPT, so their underlying functions warrant detailed study. Here, tea leaves were fermented by Aspergillus niger, Aspergillus tamarii and Aspergillus fumigatus, and resulting samples (designated as Asn, Ast and Asf, respectively) were analyzed by proteomic and metabolomic methods. Changes to the composition of flavonoids, glycerophospholipids, organo-oxygen compounds and fatty acids resulting from Aspergillus fermentation were observed. Carbohydrate-active enzymes, e.g., endoglucanases and cellulases, for degradation of cellulose, starch, lignin, pectin, xylan and xyloglucan were identified. Glycoside hydrolase, glycosyltransferases, tannase, laccases, vanillyl-alcohol oxidases and benzoquinone reductase were identified and hypothesized to catalyze hydrolysis, oxidation, polymerization and degradation of phenolic compounds. Together, functions of Aspergillius were demonstrated as production of enzymes to change concentrations and compositions of metabolites in tea leaves.


Asunto(s)
Aspergillus/fisiología , Camellia sinensis/microbiología , Enzimas/metabolismo , Hojas de la Planta/microbiología , , Aspergillus/enzimología , Aspergillus fumigatus/enzimología , Aspergillus fumigatus/fisiología , Aspergillus niger/enzimología , Aspergillus niger/fisiología , Metabolismo de los Hidratos de Carbono , Fermentación , Flavonoides/análisis , Flavonoides/metabolismo , Microbiología de Alimentos/métodos , Proteínas Fúngicas/metabolismo , Glicerofosfolípidos/metabolismo , Metabolómica/métodos , Fenoles/análisis , Fenoles/metabolismo , Hojas de la Planta/química , Hojas de la Planta/metabolismo , Proteínas de Plantas/análisis , Proteínas de Plantas/metabolismo , Proteómica/métodos , Té/química , Té/metabolismo , Té/microbiología
2.
J Diabetes Investig ; 2020 Nov 27.
Artículo en Inglés | MEDLINE | ID: mdl-33244871

RESUMEN

AIMS: To explore relationships between polyunsaturated fatty acids (PUFA) and nonalcoholic fatty liver disease (NAFLD) in patients with type 2 diabetes (T2D) and whether insulin action had an interactive effect with PUFA on NAFLD progression. MATERIALS AND METHODS: We extracted clinical and omics data of 482 T2D patients from tertiary hospital consecutively from April 2018 to April 2019. NAFLD was estimated by ultrasound at admission. Plasma fasting n3 and n6 fatty acids were quantified by liquid chromatography-tandem mass spectrometry analysis. Restricted cubic spline nested in binary logistic regression was used to select cutoff point and estimate odds ratios (OR) and 95% confidence intervals (CI). Additive interactions of n6/n3 ratio with insulin action for NAFLD were estimated using relative excess risk due to interaction (RERI), attributable proportion due to interaction (AP), and synergy index (S). RERI > 0, AP > 0, or S >1 indicates biological interaction. Spearman correlation analysis was used to obtain partial correlation coefficients between PUFA and hallmarks of NAFLD. RESULTS: Of 482 patients, 313 were with and 169 were without NAFLD. N3 ≥ 800 and n6 PUFA ≥ 8100 µmol/L were independently associated with increased NAFLD risk; n6/n3 ratio ≤10 was associated with NAFLD (1.80, 1.20-2.71) and the effect size was amplified by high C-peptide (8.89, 4.48-17.7) with significant interaction. The additive interaction of n6/n3 ratio and fasting insulin was not significant. CONCLUSION: Decreased n6/n3 ratio was associated with increased NAFLD risk in T2D, and the effect was only significant and amplified when copresence with high C-peptide.

3.
Huan Jing Ke Xue ; 40(7): 3369-3377, 2019 Jul 08.
Artículo en Chino | MEDLINE | ID: mdl-31854740

RESUMEN

To study the vertical distribution characteristics of polycyclic aromatic hydrocarbons (PAHs) in the soils from different land use types during urbanization, three land use types (urban land, cultivated land, and woodland) were selected in the eastern part of Shenyang, where urbanization is occurring rapidly. In each case, five soil samples were obtained from different depths (0-1 m). Change in the concentrations of PAHs, vertical migration factors, and the distribution and sources of PAHs were analyzed in the vertical soil profiles. Total concentrations of PAHs in the different soil type were ordered as follows:city 1 (513.19-12689.04 µg·kg-1); dry field (36.18-7196.10 µg·kg-1); paddy field (70.92-747.53 µg·kg-1); city 2 (19.39-636.47 µg·kg-1); and woodland (4.79-349.24 µg·kg-1). PAHs were mainly trapped in shallow soils in urban and forest land, but can migrate deeper into the soil profile in cultivated land. High-ring PAHs were abundant at depths of 0-30 cm, while low-ring PAHs were abundant deeper in the soil profiles. SOM had a significant effect on the vertical distribution of PAHs, and the physical and chemical properties of PAHs had a significant influence on their migration ability. However, combustion sources from industrial activities and transportation in the region are still considered the main sources of PAHs despite the fact that some low-ring PAHs derive from petroleum product inputs.

4.
Huan Jing Ke Xue ; 40(1): 248-255, 2019 Jan 08.
Artículo en Chino | MEDLINE | ID: mdl-30628281

RESUMEN

To protect the safety of water used by the residents in Shen-Fu New City, which is undergoing the process of urbanization, 49 groundwater samples were collected along the Hunhe River Basin and 16 US EPA priority control polycyclic aromatic hydrocarbons (PAHs) were analyzed. The occurrence, distribution characteristics, sources, and potential health risk of drinking the groundwater were also assessed in this study. The results show that PAHs were detected in all samples. The concentration of PAHs ranges from 4.38 to 2005.02 ng·L-1, with an average value of (414.64±526.13) ng·L-1. Based on the comparison of the concentration level with that of other regions, the results in this study indicate a higher pollution level. The 3-4 ring PAHs are dominant; the average value was (190.93±238.96) ng·L-1 and (140.01±234.69) ng·L-1, respectively, accounting for 80% of the total PAHs. The distribution of PAHs in the groundwater is affected by the land use types. The concentration of PAHs is higher when the land use type is cultivated land, while it is lower when it is forest land. The source of PAHs was identified using Principal Component Analysis-Multiple Linear Regression (PCA-MLR). It was revealed that 36.26% of the PAHs are due to incomplete combustion of petroleum and gas, 32.72% are due to coal combustion, 28.17% originate from petroleum spills, and 2.87% are due to traffic emissions. The cancer risk levels releated to drinking the groundwater range from 5.55×10-10 to 5.65×10-6 and 13.60% of the values is in the range of 10-6-10-4. The levels are higher than the baseline of the acceptable risk, indicating the potential cancer risk. More attention should be paid to the quality of the groundwater.

5.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 26(4): 1027-1032, 2018 Aug.
Artículo en Chino | MEDLINE | ID: mdl-30111402

RESUMEN

OBJECTIVE: To explore the biological function of BMAL1 in human acute myeloid leukemia by means of the HL-60 cell line in whica circadian gene BMAL1 was konocked-out by the CRISPR/Cas9 technology. METHODS: Two sgRNAs for BMAL1 were designed and the PX459 knockout vectors containing the sgRNA were constructed. The activity of 2 sgRNAs was detected by T7 endonuclease I. the BMAL1 knocked out HL-60 cells were prepared by transient transfection of the target vectors into the cells. Western blot was used to detect the expression of BMAL1 protein. The apoptosis of the targeted cells was detected by flow cytometry. The proliferation status of the cells was assessed by the CCK-8 assay. RESULTS: The PX459-sgRNA vectors were successfully constructed and screened to assure the activity of the targeting vector. It was found that the expression of BMAL1 protein was not detected in BMAL1-knocked out HL- 60 cells. Further, it was shown that BMAL1 knockdout could promote the apoptosis of HL-60 cells and inhibit the cell proliferation ability. CONCLUSION: BMAL1 knocked out HL-60 cells have bean successfully established using the CRISPR/Cas9 gene editing technique, and BMAL1 knockout can promote the HL-60 cell apoptosis and inhibit its proliferation.These result reveal the biological role of the BMAL1 circadian gene in acute myeloid leukemia.


Asunto(s)
Apoptosis , Proliferación Celular , Células HL-60 , Humanos , Leucemia Mieloide Aguda , Transfección
6.
Huan Jing Ke Xue ; 39(2): 889-898, 2018 Feb 08.
Artículo en Chino | MEDLINE | ID: mdl-29964855

RESUMEN

To study the effects of urbanization on concentration, sources, and health risks of polycyclic aromatic hydrocarbons (PAHs), 95 topsoil samples were collected from Liaoning Province. For this assessment, 21 PAHs were analyzed via gas chromatography-mass spectrometry and discussed. The results show the total concentrations of the 21 PAHs in background, Shen-Fu, Shen-Yang, and Fu-Shun were 1496.76 µg·kg-1, 3000.50 µg·kg-1, 8705.11 µg·kg-1, and 8178.90 µg·kg-1, respectively, which is positively correlated with the urbanization levels. Diagnostic ratios and PMF modal analysis indicate that the sources of the PAHs in soils were most likely coal combustion and traffic combustion (petroleum combustion). The main sources of PAHs in the four areas were petrochemical combustion (41.0%), coal combustion (64.4%), traffic combustion (67.5%), and traffic combustion (62.0%), that is, with the urbanization process, human activity (mainly traffic combustion) gradually become the major contributor of PAHs to the environment. Health risk assessment showed that the risk of cancer exposure through soil digestion and skin exposure was higher. There is a higher health risk from PAHs in urban soils, and the health risks associated with children and adolescents are greater than with other residents, requiring more attention.


Asunto(s)
Hidrocarburos Policíclicos Aromáticos/análisis , Contaminantes del Suelo/análisis , Urbanización , Adolescente , Niño , China , Carbón Mineral , Exposición a Riesgos Ambientales/efectos adversos , Monitoreo del Ambiente , Humanos , Medición de Riesgo , Suelo , Emisiones de Vehículos
7.
Huan Jing Ke Xue ; 38(2): 703-710, 2017 Feb 08.
Artículo en Chino | MEDLINE | ID: mdl-29964529

RESUMEN

The concentration level of polycyclic aromatic hydrocarbon (PAHs) in topsoil from Shenfu New City in urbanization was studied. 54 topsoil samples were collected from Shenfu New City and 4 different land uses (urban land, rural land, forest land, cultivated land) were included. The concentration of PAHs in different land uses was analyzed, the sources of soil PAHs were analyzed by using diagnostic ratios and principal component analysis, and the ecological risks were calculated using toxic equivalency concentrations of BaP and the incremental lifetime cancer risk(ILCR)model. Results indicated that the concentrations of ∑PAHs in urban land, cultivated land, rural land and forest land were in the range of 184-18276, 230-14102, 151-3205, 303-2980 µg·kg-1, respectively. Four land uses had similar sources, the main sources were coal combustion and traffic emission. According to the calculation of ecological risks, there were potential health risks for residents in Shenfu New City, especially urban land, meanwhile, residents were easily exposed to soil PAHs through dermal contact.


Asunto(s)
Exposición a Riesgos Ambientales/análisis , Hidrocarburos Policíclicos Aromáticos/análisis , Medición de Riesgo , Contaminantes del Suelo/análisis , China , Ciudades , Monitoreo del Ambiente , Humanos , Suelo
8.
Biomed Res Int ; 2015: 256365, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26273601

RESUMEN

The measurement of lower back mobility is essential in the assessment of lower back pain including ankylosing spondylitis. Original Schober's test (OST) and modified Schober's test (MST) are popularly conducted in daily rheumatology and orthopedics clinical practices. To our knowledge, this report is the only anthropometric reference study in a normal oriental population. The OST declined with age from 5.0 cm in the youngest (20-30 years old) to 3.1 cm in the aged (70-80 years old) male subjects and from 3.6 cm to 2.4 cm in the female subjects. The male OST was significantly more than the female OST. There was a good correlation between OST and MST in each of the three age groups of both sexes.


Asunto(s)
Envejecimiento/fisiología , Antropometría/métodos , Región Lumbosacra/fisiología , Examen Físico/métodos , Rango del Movimiento Articular/fisiología , Adulto , Distribución por Edad , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Valores de Referencia , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Distribución por Sexo , Factores Sexuales , Taiwán/epidemiología , Adulto Joven
9.
Fitoterapia ; 100: 195-200, 2015 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-25481372

RESUMEN

Four new nardosinane-type sesquiterpenoids nardosinanones F-I (1-2, 4-5), along with eight known sesquiterpenoids (3, 6-12) were isolated from the underground parts of Nardostachys chinensis Batal. Their structures were elucidated on the basis of extensive spectroscopic analysis. Compounds 1 and 2 were new nardosinane sesquiterpenoids processing a rare 4,11-epoxy group in nature. In addition, compounds 1, 5-7, 11 and 12 showed protective effects on neonatal rat cardiomyocyte injury induced by hydrogen peroxide.


Asunto(s)
Miocitos Cardíacos/efectos de los fármacos , Nardostachys/química , Sesquiterpenos/química , Animales , Células Cultivadas , Peróxido de Hidrógeno/efectos adversos , Estructura Molecular , Raíces de Plantas/química , Ratas Wistar , Sesquiterpenos/aislamiento & purificación
10.
CNS Neurosci Ther ; 21(3): 252-61, 2015 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-25438992

RESUMEN

BACKGROUND: Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor with a dismal prognosis. Despite intensive study on tumor biology, the underlying mechanisms of the unlimited proliferation and progressive local invasion are still poorly understood, and no effective treatment has been developed for GBM patients. AIMS: We determine the role of TRPM7 channels in the growth, migration, and infiltration of malignant glioma cells. METHODS: Using a combination of RT-PCR, Western blot, and patch-clamp techniques, we demonstrated the expression of functional TRPM7 channels of A172 cells, a human glioma cell line, as well as in human glioma tissues. Furthermore, we evaluated the role of TRPM7 in growth, migration, and infiltration of A172 cells with MTT and transwell migration and invasion assays. RESULTS: We showed the expression of functional TRPM7 channels in both A172 cells and human glioma tissues. Suppression of TRPM7 expression with TRPM7-siRNA dramatically reduced the proliferation, migration, and invasion of A172 cells. Pharmacological inhibition of TRPM7 channel with 2-aminoethoxydiphenyl borate (2-APB) showed a similar effect as TRPM7-siRNA. CONCLUSION: We demonstrate that human glioma cells express functional TRPM7 channel and that activation of this channel plays an important role in the proliferation, migration, and invasion of malignant glioma cells. TRPM7 channel may represent a novel and promising target for therapeutic intervention of malignant glioma.


Asunto(s)
Neoplasias Encefálicas/fisiopatología , Movimiento Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Glioblastoma/fisiopatología , Invasividad Neoplásica/fisiopatología , Proteínas Serina-Treonina Quinasas/antagonistas & inhibidores , Canales Catiónicos TRPM/antagonistas & inhibidores , Western Blotting , Compuestos de Boro/farmacología , Neoplasias Encefálicas/tratamiento farmacológico , Línea Celular Tumoral , Movimiento Celular/fisiología , Proliferación Celular/fisiología , Células Cultivadas , Glioblastoma/tratamiento farmacológico , Humanos , Técnicas de Placa-Clamp , Reacción en Cadena de la Polimerasa , Proteínas Serina-Treonina Quinasas/genética , Proteínas Serina-Treonina Quinasas/metabolismo , ARN Mensajero/metabolismo , ARN Interferente Pequeño , Canales Catiónicos TRPM/genética , Canales Catiónicos TRPM/metabolismo
11.
Chin J Nat Med ; 12(9): 697-9, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-25263983

RESUMEN

Phytochemical investigation on Forsythia suspensa (Thunb.) Vahl afforded ten compounds, including five lignan glycosides and five phenylethanoid glycosides. The compounds were isolated by using HP-20 macroporous resin, silica gel, octadecyl silica gel (ODS), size exclusion chromatography resin HW-40 chromatography, and preparative HPLC. The structures were established through application of extensive spectroscopic methods, including ESI-MS, 1D-and 2D-NMR spectroscopy. They were identified as forsythialanside E (1), 8'-hydroxypinoresinol-4'-O-ß-D-glucoside (2), 8'-hydroxypinoresinol (3), lariciresinol-4'-O-ß-D-glucoside (4), lariciresinol-4-O-ß-D-glucoside (5), forsythoside H (6), forsythoside I (7), forsythoside F (8), plantainoside B (9), and plantainoside A (10). Compound 1 was a new lignan glycoside.


Asunto(s)
Forsythia/química , Glicósidos/aislamiento & purificación , Lignanos/aislamiento & purificación , Extractos Vegetales/química , Glicósidos/química , Lignanos/química , Estructura Molecular
12.
Phytochemistry ; 104: 105-13, 2014 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-24833035

RESUMEN

Phytochemical investigation on Forsythia suspensa (Thunb.) Vahl afforded 10 compounds, including quinoid glycosides, lignan glycosides, phenylethanoid glycoside and allylbenzene glycoside together with 13 known ones. Their structures were established based on extensive spectroscopic data analyses, including IR, UV, HRESIMS, 1D NMR and 2D NMR. Absolute configurations were determined by ECD calculation method and chemical degradation. In addition, all compounds were evaluated for their antiviral activity against influenza A (H1N1) virus and several were further evaluated against respiratory syncytial virus (RSV) in vitro. Among them, two previously known compounds showed significant activities against RSV with EC50 values of 3.43 and 6.72 µM.


Asunto(s)
Antivirales/farmacología , Forsythia/química , Glicósidos/farmacología , Subtipo H1N1 del Virus de la Influenza A/efectos de los fármacos , Lignanos/farmacología , Alcohol Feniletílico/farmacología , Animales , Antivirales/química , Antivirales/aislamiento & purificación , Línea Celular , Perros , Frutas/química , Glicósidos/química , Glicósidos/aislamiento & purificación , Humanos , Lignanos/química , Lignanos/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Estructura Molecular , Alcohol Feniletílico/química , Alcohol Feniletílico/aislamiento & purificación , Virus Sincitiales Respiratorios/efectos de los fármacos
13.
Org Lett ; 15(5): 1000-3, 2013 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-23394111

RESUMEN

Nardoaristolones A and B, two novel terpenoids derived from the aristolane-type sesquiterpenoid, were isolated from the underground parts of Nardostachys chinensis Batal. Nardoaristolone A is the first reported aristolane-chalcone derivative, while nardoaristolone B possesses a nor-aristolane sesquiterpenoid skeleton with an unusual 3/5/6 tricyclic ring system. Their structures were elucidated by spectroscopic measurements, and the absolute configurations were established by single-crystal X-ray diffraction experiments.


Asunto(s)
Nardostachys/química , Sesquiterpenos/química , Sesquiterpenos/aislamiento & purificación , Animales , Cristalografía por Rayos X , Peróxido de Hidrógeno/farmacología , Conformación Molecular , Estructura Molecular , Miocitos Cardíacos/efectos de los fármacos , Raíces de Plantas/química , Ratas
14.
J Fluoresc ; 23(1): 7-12, 2013 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-22926430

RESUMEN

A rare earth ytterbium complex with 2,4,6-pyridinetricarboxylic acid (H(3)pta) has been synthesized by hydrothermal method, the formula is {[Yb(2)(pta)(2)(H(2)O)(3)]·H(2)O}(n). The complex crystallized in monoclinic system, P2(1)/c space group with lattice parameters a = 11.6556(19)Å, b = 7.8364(12), c = 22.020(4), α = γ = 90º, ß = 92.120(3), Z = 4, GOF = 1.026, R1 = 0.0334, wR2 = 0.0660. The pta anions connect four rare earth Yb(III) ions with two different coordination modes. The complex exhibit intense characteristic near-infrared luminescence of Yb(III) ions at 990 nm with excitation of UV-rays.

15.
Fertil Steril ; 99(1): 86-91, 2013 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-22985948

RESUMEN

OBJECTIVE: To determine association between defective protein kinases C (PKC) and A (PKA) and disordered zona pellucida (ZP)-induced acrosome reaction (DZPIAR) in normozoospermic infertile men with normal sperm-ZP binding. DESIGN: Sperm from DZPIAR infertile men were treated without (control) or with (test) phorbol myristate acetate (PMA, PKC activator) or dibutyryl cyclic AMP (dbcAMP, PKA activator) under in vitro standard culture condition. The ZP-induced AR was assessed and compared between control and test. SETTING: Public and private hospital-based clinical assisted reproduction technology (ART) centers. PATIENT(S): A total of 51 DZPIAR infertile men were involved in this study. INTERVENTION(S): None. MAIN OUTCOMES MEASURE(S): Sperm-ZP binding and the ZP-induced IAR. RESULT(S): Both PMA and dbcAMP enhanced ZP-induced AR up to a normal level (≥25%) in some subjects with DZPIAR: 29 (57%) with PMA and 27 (53%) with dbcAMP. Overall 35 (69%) had the ZP-induced AR enhanced to normal by PMA or dbcAMP but 16 (31%) had little or no response to either agent. Fourteen men responded to the two activators differently: 8 effective only with PMA and 6 effective only with dbcAMP. CONCLUSION(S): Defective upstream of PKC and PKA pathways are highly associated with disordered ZPIAR in normozoospermic infertile men with normal sperm-ZP binding.


Asunto(s)
Reacción Acrosómica/fisiología , Proteínas Quinasas Dependientes de AMP Cíclico/fisiología , Infertilidad Masculina/fisiopatología , Proteína Quinasa C/fisiología , Transducción de Señal/fisiología , Interacciones Espermatozoide-Óvulo/fisiología , Zona Pelúcida/fisiología , Reacción Acrosómica/efectos de los fármacos , Bucladesina/farmacología , Humanos , Técnicas In Vitro , Masculino , Interacciones Espermatozoide-Óvulo/efectos de los fármacos , Espermatozoides/efectos de los fármacos , Espermatozoides/fisiología , Acetato de Tetradecanoilforbol/farmacología
16.
Fertil Steril ; 99(2): 367-71, 2013 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-23089238

RESUMEN

OBJECTIVE: To determine differences and frequency of excessive (≥50%) sperm DNA damage between ejaculated and motile sperm. DESIGN: Sperm DNA damage was assessed by acridine orange fluorescence and the results of ejaculated and motile sperm were compared. SETTING: Public and private clinical assisted reproduction centers. PATIENT(S): A total of 272 subfertile men were studied. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Semen analysis and sperm DNA damage. RESULT(S): Sperm DNA damage was negatively correlated with sperm motility and normal morphology. Overall, 39.7% (108 of 272) of semen samples had excessive sperm DNA damage. In contrast, only 15% (41 of 272) of motile sperm fractions had excessive DNA damage. Based on DNA results of motile sperm and semen characteristics, the proportion of men with excessive sperm DNA damage was 26% in severe teratozoospermia, 17.5% in oligozoospermia, 12.5% in moderate teratozoospermia, and 4.6% in normozoospermia. Severe teratozoospermia had five times more frequent excessive DNA damage than normozoospermia. CONCLUSION(S): Abnormal sperm morphology is highly associated with sperm DNA damage. Results of DNA damage of ejaculated sperm do not accurately reflect DNA status of motile sperm. Therefore, sperm DNA damage should be assessed in motile sperm fraction rather than whole ejaculated sperm.


Asunto(s)
Daño del ADN/genética , Análisis Mutacional de ADN/métodos , ADN/genética , Eyaculación/genética , Oligospermia/genética , Análisis de Semen/métodos , Motilidad Espermática/genética , Adulto , ADN/análisis , Humanos , Masculino , Persona de Mediana Edad , Oligospermia/diagnóstico , Reproducibilidad de los Resultados , Sensibilidad y Especificidad
17.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 47(3): 186-9, 2012 Mar.
Artículo en Chino | MEDLINE | ID: mdl-22800675

RESUMEN

OBJECTIVE: To explore the computer aided design (CAD) method of veneer restoration, and to assess if the solution can help prosthesis meet morphology esthetics standard. METHODS: A volunteer's upper right central incisor needed to be restored with veneer. Super hard stone models of patient's dentition (before and after tooth preparation) were scanned with the three-dimensional laser scanner. The veneer margin was designed as butt-to-butt type. The veneer was constructed using reverse engineering (RE) software. RESULTS: The technique guideline of veneers CAD was explore based on RE software, and the veneers was smooth, continuous and symmetrical, which met esthetics construction needs. CONCLUSIONS: It was a feasible method to reconstruct veneer restoration based on RE technology.


Asunto(s)
Ingeniería Biomédica/métodos , Diseño Asistido por Computadora , Coronas con Frente Estético , Incisivo , Diseño de Prótesis Dental/métodos , Humanos
18.
Reprod Biomed Online ; 23(6): 735-9, 2011 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-22036189

RESUMEN

The aim of this study was to compare sperm velocity, hyperactivation, zona pellucida (ZP) binding and ZP-induced acrosome reaction (AR) between Quinn's advantage fertilization (QAF), human tubal fluid (HTF) and Ham's F10 media. Semen samples were obtained from normozoospermic men and motile spermatozoa were prepared by gradient centrifugation (PureSperm). Unfertilized oocytes from clinical IVF were used for spermatozoa-oocyte interaction tests. Sperm velocity and hyperactivation were assessed using a Hamilton-Thorn motility analyser. When media were supplemented with human albumin, sperm motility and velocity and sperm binding were not significantly different between QAF and HTF. However, ZP-induced AR was significantly higher with QAF than HTF (42±22 versus 21±18, P<0.th001). Sperm velocity, hyperactivation and sperm binding were also significantly higher in QAF than Ham's F10 media. Supplementation of media with either human serum or human albumin showed no difference in effect on all sperm test results. In conclusion, QAF medium significantly enhances ZP-induced AR which is essential for sperm penetration. Thus QAF appears to be a better medium than HTF for sperm fertilizing ability in conventional IVF.


Asunto(s)
Reacción Acrosómica/efectos de los fármacos , Medios de Cultivo/farmacología , Fertilización In Vitro/métodos , Análisis de Semen , Zona Pelúcida/efectos de los fármacos , Albúminas , Líquidos Corporales , Técnicas de Cultivo de Célula , Técnicas de Cultivo de Embriones , Trompas Uterinas , Femenino , Humanos , Masculino , Suero , Capacitación Espermática/efectos de los fármacos , Espermatozoides/efectos de los fármacos , Espermatozoides/fisiología , Zona Pelúcida/fisiología
19.
Asian J Androl ; 11(4): 499-507, 2009 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-19434054

RESUMEN

The aim of this study was to determine the relationship between seminal zinc concentration and spermatozoa-zona pellucida (ZP) binding and the ZP-induced acrosome reaction (ZPIAR) in subfertile men. Semen analyses and seminal zinc concentration assessments were carried out according to the World Health Organization manual for 458 subfertile men. A spermatozoa-ZP interaction test was carried out by incubating 2 x 10(6) motile spermatozoa with a group of four unfertilized oocytes obtained from a clinical in vitro fertilization programme. After 2 h of incubation, the number of spermatozoa bound per ZP and the ZPIAR of ZP-bound spermatozoa were examined. The effect of adding 0.5 mmol L(-1) zinc to the media on the ZPIAR of spermatozoa from normozoospermic men was also tested in vitro. Seminal zinc concentration positively correlated with sperm count and duration of abstinence, but negatively correlated with semen volume. On analysis of data from all participants, both spermatozoa-ZP binding and the ZPIAR were significantly correlated with sperm motility and normal morphology, but not with seminal zinc concentration. However, in men with normozoospermic semen, the seminal zinc concentration was significantly higher in men with defective ZPIAR (< 16%) than in those with normal ZPIAR (>or= 16%) (P < 0.01). The addition of 0.5 mmol L(-1) zinc to the culture media had no effect on spermatozoa-ZP binding, but significantly reduced the ZPIAR in vitro (P < 0.001). In conclusion, seminal zinc concentration is correlated with sperm count and the duration of abstinence in subfertile men. In men with normozoospermic semen, high seminal zinc concentration may have an adverse effect on the ZPIAR.


Asunto(s)
Reacción Acrosómica , Infertilidad Masculina/metabolismo , Semen/metabolismo , Espermatozoides/metabolismo , Zinc/metabolismo , Zona Pelúcida/metabolismo , Humanos , Masculino , Motilidad Espermática , Interacciones Espermatozoide-Óvulo
20.
Dalton Trans ; (35): 4711-3, 2008 Sep 21.
Artículo en Inglés | MEDLINE | ID: mdl-18728877

RESUMEN

Using a series flexible thioether ligands, 4-(2-pyridylmethylthio)benzoic acid (HL(1)), 4-(4-pyridylmethylthio)benzoic acid (HL(2)) and 4-(3-pyridylmethylthio)benzoic acid (HL(3)), a 1D infinite chain [Zn(3)(L(1))(6)](n) (1), a 2D interpenetrating sheet [Zn(L(2))(2)](n) (2), and a chiral 3D framework [Zn(L(3))(2)H(2)O](n) (3) were obtained. Luminescent properties of these compounds were also studied.

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