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1.
Psychiatry Res ; 300: 113899, 2021 Mar 23.
Artículo en Inglés | MEDLINE | ID: mdl-33812218

RESUMEN

Previous studies have shown that berberine can improve metabolic disturbances in non-psychiatric patients, but no clinical research has been conducted in schizophrenia. This study was a randomized, double-blind, placebo-controlled clinical trial. Eligible patients diagnosed with schizophrenia were randomized to receive placebo or berberine (900mg/day) as an adjunctive treatment for eight weeks. Peripheral glycolipid metabolism parameters were measured at baseline, week 4, and week 8. Sixty-five patients were included, and forty-nine patients completed the 8-week trial. Berberine led to significant declines in total cholesterol, low-density lipoprotein cholesterol, fasting serum insulin, and insulin resistance(all p<0.05) compared with placebo. Baseline body mass index and serum prolactin concentration could predict the effect of berberine on insulin resistance. Berberine adjunctive treatment may reduce the risk of glycolipid metabolic disturbances in patients with schizophrenia.

2.
J Control Release ; 2021 Mar 30.
Artículo en Inglés | MEDLINE | ID: mdl-33798666

RESUMEN

Breast cancer metastasis and recurrence accounts for vast majority of breast cancer-induced mortality. Tumor microenvironment (TME) plays an important role at each step of metastasis, evasion of immunosurveillance, and therapeutic resistance. Consequently, TME-targeting alternatives to traditional therapies focused on breast cancer cells are gaining increasing attention. These new therapies involve the use of tumor cells, and key TME components or secreted bioactive molecules as therapeutic targets, alone or in combination. Recently, TME-related nanoparticles have been developed to deliver various agents, such as bioactive ingredients extracted from natural sources or chemotherapeutic agents, genes, proteins, small interfering RNAs, and vaccines; they have shown great therapeutic potential against breast cancer metastasis. Among various types of nanoparticles, biomimetic nanovesicles are a promising means of addressing the limitations of conventional nanocarriers. This review highlights various nanoparticles related to or mediated by TME according to the key TME components responsible for metastasis. Furthermore, TME-related biomimetic nanoparticles against breast cancer metastasis have garnered attention owing to their promising efficiency, especially in payload delivery and therapeutic action. Here, we summarize recent representative studies on nanoparticles related to cancer-associated fibroblasts, extracellular matrix, endothelial cells, angiogenesis, and immune cells, as well as advanced biomimetic nanoparticles. Future challenges and opportunities in the field are also discussed.

3.
Sensors (Basel) ; 21(6)2021 Mar 14.
Artículo en Inglés | MEDLINE | ID: mdl-33799464

RESUMEN

Precise localization is critical to safety for connected and automated vehicles (CAV). The global navigation satellite system is the most common vehicle positioning method and has been widely studied to improve localization accuracy. In addition to single-vehicle localization, some recently developed CAV applications require accurate measurement of the inter-vehicle distance (IVD). Thus, this paper proposes a cooperative localization framework that shares the absolute position or pseudorange by using V2X communication devices to estimate the IVD. Four IVD estimation methods are presented: Absolute Position Differencing (APD), Pseudorange Differencing (PD), Single Differencing (SD) and Double Differencing (DD). Several static and dynamic experiments are conducted to evaluate and compare their measurement accuracy. The results show that the proposed methods may have different performances under different conditions. The DD shows the superior performance among the four methods if the uncorrelated errors are small or negligible (static experiment or dynamic experiment with open-sky conditions). When multi-path errors emerge due to the blocked GPS signal, the PD method using the original pseudorange is more effective because the uncorrelated errors cannot be eliminated by the differential technique.

4.
Entropy (Basel) ; 23(4)2021 Mar 28.
Artículo en Inglés | MEDLINE | ID: mdl-33800642

RESUMEN

The problem of extracting meaningful data through graph analysis spans a range of different fields, such as social networks, knowledge graphs, citation networks, the World Wide Web, and so on. As increasingly structured data become available, the importance of being able to effectively mine and learn from such data continues to grow. In this paper, we propose the multi-scale aggregation graph neural network based on feature similarity (MAGN), a novel graph neural network defined in the vertex domain. Our model provides a simple and general semi-supervised learning method for graph-structured data, in which only a very small part of the data is labeled as the training set. We first construct a similarity matrix by calculating the similarity of original features between all adjacent node pairs, and then generate a set of feature extractors utilizing the similarity matrix to perform multi-scale feature propagation on graphs. The output of multi-scale feature propagation is finally aggregated by using the mean-pooling operation. Our method aims to improve the model representation ability via multi-scale neighborhood aggregation based on feature similarity. Extensive experimental evaluation on various open benchmarks shows the competitive performance of our method compared to a variety of popular architectures.

5.
Vaccines (Basel) ; 9(4)2021 Mar 25.
Artículo en Inglés | MEDLINE | ID: mdl-33805985

RESUMEN

We sought to analyze the evolutionary characteristics and neutralization sensitivity of viruses in a human immunodeficiency virus type 1 (HIV-1) subtype B' infected plasma donor with broadly neutralizing activity, which may provide information for new broadly neutralizing antibodies (bNAbs) isolation and immunogen design. A total of 83 full-length envelope genes were obtained by single-genome amplification (SGA) from the patient's plasma at three consecutive time points (2005, 2006, and 2008) spanning four years. In addition, 28 Env-pseudotyped viruses were constructed and their neutralization sensitivity to autologous plasma and several representative bNAbs were measured. Phylogenetic analysis showed that these env sequences formed two evolutionary clusters (Cluster I and II). Cluster I viruses vanished in 2006 and then appeared as recombinants two years later. In Cluster II viruses, the V1 length and N-glycosylation sites increased over the four years of the study period. Most viruses were sensitive to concurrent and subsequent autologous plasma, and to bNAbs, including 10E8, PGT121, VRC01, and 12A21, but all viruses were resistant to PGT135. Overall, 90% of Cluster I viruses were resistant to 2G12, while 94% of Cluster II viruses were sensitive to 2G12. We confirmed that HIV-1 continued to evolve even in the presence of bNAbs, and two virus clusters in this donor adopted different escape mechanisms under the same humoral immune pressure.

6.
Polymers (Basel) ; 13(6)2021 Mar 23.
Artículo en Inglés | MEDLINE | ID: mdl-33806971

RESUMEN

Fire-protection coatings with a self-monitoring ability play a critical role in safety and security. An intelligent fire-protection coating can protect humans from personal and property damage. In this work, we report the fabrication of a low-cost and facile intelligent fire coating based on a composite of ammonium polyphosphate and epoxy (APP/EP). The composite was processed using laser scribing, which led to a laser-induced graphene (LIG) layer on the APP/EP surface via a photothermal effect. The C-O, C=O, P-O, and N-C bonds in the flame-retardant APP/EP composite were broken during the laser scribing, while the remaining carbon atoms recombined to generate the graphene layer. A proof-of-concept was achieved by demonstrating the use of LIG in supercapacitors, as a temperature sensor, and as a hazard detection device based on the shape memory effect of the APP/EP composite. The intelligent flame protection coating had a high flame retardancy, which increased the time to ignition (TTI) from 21 s to 57 s, and the limiting oxygen index (LOI) value increased to 37%. The total amount of heat and smoke released during combustion was effectively suppressed by ≈ 71.1% and ≈ 74.1%, respectively. The maximum mass-specific supercapacitance could reach 245.6 F·g-1. The additional LIG layer enables applications of the device as a LIG-APP/EP temperature sensor and allows for monitoring of the deformation according to its shape memory effect. The direct laser scribing of graphene from APP/EP in an air atmosphere provides a convenient and practical approach for the fabrication of flame-retardant electronics.

7.
Artículo en Inglés | MEDLINE | ID: mdl-33811024

RESUMEN

Selenium (Se) deficiency affects many millions of people worldwide, and the volatilization of methylated Se species to the atmosphere may prevent Se from entering the food chain. Despite the extent of Se deficiency, little is known about fluxes in volatile Se species and their temporal and spatial variation in the environment, giving rise to uncertainty in atmospheric transport models. To systematically determine fluxes, one can rely on laboratory microcosm experiments to quantify Se volatilization in different conditions. Here, it is demonstrated that the sulfur (S) status of bacteria crucially determines the amount of Se volatilized. Solid phase microextraction gas chromatography mass spectrometry showed that Pseudomonas tolaasii efficiently and rapidly (92% in 18h) volatilized Se to dimethyldiselenide and dimethylselenylsulfide through promiscuous enzymatic reactions with the S metabolism. However, when the cells were supplemented with cystine (but not methionine), a major proportion of the Se (∼48%) was channelled to thus far unknown, non-volatile Se compounds at the expense of the previously formed dimethyldiselenide and dimethylselenylsulfide (accounting for < 4% of total Se). Ion chromatography and solid phase extraction were used to isolate unknowns, and electrospray ionization ion trap mass spectrometry, electrospray ionization quadrupole time-of-flight mass spectrometry and microprobe nuclear magnetic resonance spectrometry were used to identify the major unknown as a novel Se metabolite, 2-hydroxy-3-(methylselanyl)propanoic acid. Environmental S concentrations often exceed Se concentrations by orders of magnitude. This suggests that in fact S status may be a major control on selenium fluxes to the atmosphere.ImportanceVolatilization from soil to the atmosphere is a major driver for Se deficiency. "Bottom up" models for atmospheric Se transport are based on laboratory experiments quantifying volatile Se compounds. The high Se and low S concentrations in such studies poorly represent the environment. Here, we show that S amino acid status has in fact a decisive effect on the production of volatile Se species in Pseudomonas tolaasii When the strain was supplemented with S amino acids, a major proportion of the Se was channelled to thus far unknown, non-volatile Se compounds at the expense of volatile compounds. This hierarchical control of the microbial S amino acid status on Se cycling has been thus far neglected. Understanding these interactions - if occurring in the environment- will help to improve atmospheric Se models and thus predict drivers of Se deficiency.

8.
Molecules ; 26(5)2021 Mar 09.
Artículo en Inglés | MEDLINE | ID: mdl-33803244

RESUMEN

Cellulose, as a natural polymer with an abundant source, has been widely used in many fields including the electric field responsive medium that we are interested in. In this work, cellulose micron particles were applied as an electrorheological (ER) material. Because of the low ER effect of the raw cellulose, a composite particle of cellulose and Laponite was prepared via a dissolution-regeneration process. Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) were used to observe the morphologies and structures of the composite particles, which were different from pristine cellulose and Laponite, respectively. The ER performances of raw cellulose and the prepared composite were measured by an Anton Paar rotational rheometer. It was found that the ER properties of the composite were more superior to those of raw cellulose due to the flake-like shapes of the composite particles with rough surface. Moreover, the sedimentation stability of composite improves drastically, which means better suspension stability.

9.
Front Immunol ; 12: 631609, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33815381

RESUMEN

Although the abilities of the omentum to alleviate inflammation and prevent infection have been revealed over the past decades, the underlying mechanisms remain largely unelucidated. Here, we demonstrated that the mortality of mice exposed to cecal ligation and puncture (CLP) and omentectomy was remarkably increased compared to those treated with CLP alone. Moreover, the efficacy of the omentum was associated with an impairment in intraperitoneal bacterial clearance together with an increase in the expression of proinflammatory cytokines. Besides, in response to peritoneal infections, the size and quantity of the omental milky spots (MSs) were increased tremendously and they also support innate-like B1 cell responses and local IgM production in the peritoneal cavity. Furthermore, not only the migration but also the functional activities of neutrophils were diminished in the absence of the omentum. These data collectively show that the omentum contributes more to peritoneal immune responses during septic peritonitis than has heretofore been recognized. Thus, harnessing the function of MS-containing omentum to increase its protective effectiveness may exert important biological and therapeutic implications for the control of intra-abdominal infections.

10.
Infect Dis Poverty ; 10(1): 45, 2021 Mar 31.
Artículo en Inglés | MEDLINE | ID: mdl-33789752

RESUMEN

BACKGROUND: The management of discharge COVID-19 patients with recurrent positive SARS-CoV-2 RNA is challenging. However, there are fewer scientific dissertations about the risk of recurrent positive. The aim of this study was to explore the relationship between SARS-COV-2 RNA positive duration (SPD) and the risk of recurrent positive. METHODS: This case-control multi-center study enrolled participants from 8 Chinese hospital including 411 participants (recurrent positive 241). Using unadjusted and multivariate-adjusted logistic regression analyses, generalized additive model with a smooth curve fitting, we evaluated the associations between SPD and risk of recurrent positive. Besides, subgroup analyses were performed to explore the potential interactions. RESULTS: Among recurrent positive patients, there were 121 females (50.2%), median age was 50 years old [interquartile range (IQR): 38-63]. In non-adjusted model and adjusted model, SPD was associated with an increased risk of recurrent positive (fully-adjusted model: OR = 1.05, 95% CI: 1.02-1.08, P = 0.001); the curve fitting was not significant (P = 0.286). Comparing with SPD < 14 days, the risk of recurrent positive in SPD > 28 days was risen substantially (OR = 3.09, 95% CI: 1.44-6.63, P = 0.004). Interaction and stratified analyses showed greater effect estimates of SPD and risk of recurrent positive in the hypertension, low monocyte count and percentage patients (P for interaction = 0.008, 0.002, 0.036, respectively). CONCLUSION: SPD was associated with a higher risk of recurrent positive and especially SPD > 28 day had a two-fold increase in the relative risk of re-positive as compared with SPD < 14 day. What's more, the risk may be higher among those with hypertension and lower monocyte count or percentage.

11.
Chemosphere ; 278: 130407, 2021 Mar 29.
Artículo en Inglés | MEDLINE | ID: mdl-33823346

RESUMEN

In this study, according to the classification of biological "classes" and the different trophic levels of the food web, the distribution characteristics, bioaccumulation of heavy metals (HMs) and their trophic transfer in the food web of typical grassland ecosystems were studied and predicted. The results indicated that the accumulation of toxic As was the highest in small mammals and reptiles, Cu was the highest in insects, and the micronutrient Zn in large mammals was higher than that in plants. The metal transfer factor (MTF) by plants at the first trophic level showed that Leymus chinensis had the best ability to absorb HMs from soil. The trophic transfer factor (TTF) of HMs in the second-trophic level insects, birds and some mammals were Zn > As > Cu > Ni > Pb > Co = Cr > Mn > V, in which, biomagnified on Zn, As, and Cu. Organisms at the third trophic level including birds, reptiles and some mammals had the strongest accumulation ability for Pb, V and As, and all were biomagnified. The biomagnification on As and Co of the fourth trophic level Siberian weasel was obviously higher than that of Dione's rat-snake, which had significant biomagnification effect on As by preying on Steppe toad-headed agama. The study showed that the bioaccumulation levels of HMs in organisms at different trophic levels varied significantly with species, prey, and organ type, but they all showed strong bioaccumulation capacity to toxic As, which indicated that As could produce certain toxic effects on animals in the food web through trophic transfer. In addition, organisms at low-trophic levels were more likely to biomagnify Zn, while organisms at high-trophic levels were more likely to biodilute Pb.

12.
Sci Rep ; 11(1): 7459, 2021 Apr 02.
Artículo en Inglés | MEDLINE | ID: mdl-33811216

RESUMEN

Although sex determination and differentiation are key developmental processes in animals, the involvement of non-coding RNA in the regulation of this process is still not clarified. The tiger pufferfish (Takifugu rubripes) is one of the most economically important marine cultured species in Asia, but analyses of miRNA and long non-coding RNA (lncRNA) at early sex differentiation stages have not been conducted yet. In our study, high-throughput sequencing technology was used to sequence transcriptome libraries from undifferentiated gonads of T. rubripes. In total, 231 (107 conserved, and 124 novel) miRNAs were obtained, while 2774 (523 conserved, and 2251 novel) lncRNAs were identified. Of these, several miRNAs and lncRNAs were predicted to be the regulators of the expression of sex-related genes (including fru-miR-15b/foxl2, novel-167, novel-318, and novel-538/dmrt1, novel-548/amh, lnc_000338, lnc_000690, lnc_000370, XLOC_021951, and XR_965485.1/gsdf). Analysis of differentially expressed miRNAs and lncRNAs showed that three mature miRNAs up-regulated and five mature miRNAs were down-regulated in male gonads compared to female gonads, while 79 lncRNAs were up-regulated and 51 were down-regulated. These findings could highlight a group of interesting miRNAs and lncRNAs for future studies and may reveal new insights into the function of miRNAs and lncRNAs in sex determination and differentiation.

13.
Chin Med J (Engl) ; 2021 Mar 31.
Artículo en Inglés | MEDLINE | ID: mdl-33813517

RESUMEN

BACKGROUND: Compared to adult studies, studies which involve the treatment of pediatric congenital hypogonadotropic hypogonadism (CHH) are limited and no universal treatment regimen is available. The aim of this study was to evaluate the feasibility of human chorionic gonadotropin (hCG)/human menopausal gonadotropin (hMG) therapy for treating male adolescents with CHH. METHODS: Male adolescent CHH patients were treated with hCG/hMG (n = 20) or a gonadotropin-releasing hormone (GnRH) pump (n = 21). The treatment was divided into a study phase (0-3 months) and a follow-up phase (3-12 months). The testicular volume (TV), penile length (PL), penis diameter (PD), and sex hormone levels were compared between the two groups. The TV and other indicators between the groups were analyzed using a t-test (equal variance) or a rank sum test (unequal variance). RESULTS: Before treatment, there was no statistical difference between the two groups in terms of the biochemistry, hormones, and other demographic indicators. After 3 months of treatment, the TV of the hCG/hMG and GnRH groups increased to 5.1 ±â€Š2.3 mL and 4.1 ±â€Š1.8 mL, respectively; however, the difference was not statistically significant (P > 0.05, t = 1.394). The PL reached 6.9 ±â€Š1.8 cm and 5.1 ±â€Š1.6 cm (P < 0.05, t = 3.083), the PD reached 2.4 ±â€Š0.5 cm and 2.0 ±â€Š0.6 cm (P < 0.05, t = 2.224), respectively, in the two groups. At the end of 6 months of treatment, biomarkers were in normal range in the two groups. Compared with the GnRH group, the testosterone (T) level and growth of PL and PD were significantly greater in the hCG/hMG group (all P < 0.05). While the TV of both groups increased, the difference was not statistically significant (P > 0.05, t = 0.314). After 9 to 12 months of treatment, the T level was higher in the hCG/hMG group. Other parameters did not exhibit a statistical difference. CONCLUSIONS: The hCG/hMG regimen is feasible and effective for treating male adolescents with CHH. The initial 3 months of treatment may be a window to optimally observe the strongest effects of therapy. Furthermore, results from the extended time-period showed positive outcomes at the 1-year mark; however, the long-term effectiveness, strengths, and weaknesses of the hCG/hMG regimen require further research. TRIAL REGISTRATION: ClinicalTrials.gov, NCT02880280; https://clinicaltrials.gov/ct2/show/NCT02880280.

14.
Int J Nurs Pract ; : e12936, 2021 Apr 05.
Artículo en Inglés | MEDLINE | ID: mdl-33817904

RESUMEN

AIM: This study aimed to develop and psychometrically test a competence-based human resource management scale to improve managerial competence of first-line nurse managers in public hospitals. BACKGROUND: To ensure the high competence of the first-line nurse managers, the competence-based human resource management should be evaluated using the best-fit instrument. However, no instrument has been identified in the hospital setting. METHODS: This methodological study consists of three main steps: (1) generating scale items based on literature review and expert interviews, (2) pilot testing of face and content validity and (3) evaluating psychometric properties with 300 first-line nurse managers from 16 public hospitals in Indonesia selected using a multistage sampling. Data were collected from May to December 2017. Principal component analysis and Cronbach's α were used for construct validity and internal consistency reliability. RESULTS: The final scale consists of 30 items in five dimensions, which explained 65.48% of the total variance. The dimensions include 'training and evaluation, career planning and development, rewards scheme, recruitment and selection, and appraisal system'. Cronbach's α for the scale was .89, with a range of .85 to .88. CONCLUSION: The competence-based human resource management scale is reliable and valid to use in public hospital settings. SUMMARY STATEMENT: What is already known about this topic? The concept of competence-based human resource management has been applied worldwide in every organization, and it is applicable to use for developing managerial competence of first-line nurse managers. Therefore, the effectiveness of this management system should be assessed via a valid and reliable instrument. Several instruments to measure competence-based human resource management have been developed, but no instrument has been identified to measure competence-based human resource management specifically in hospital settings. What this paper adds? This study developed a scale designed to measure competence-based human resource management to support improvement of first-line nurse managers' managerial competence in public hospitals. The scale demonstrated sound psychometric properties with acceptable internal consistency reliability, content validity and construct validity. The scale consists of 30 items in five dimensions: training and evaluation, career planning and development, rewards scheme, recruitment and selection, and appraisal system. The implications of this paper: This study expands the basic knowledge of competence-based human resource management as a concept with five dimensions that can be used in nursing organizations, and it can be used in quality assurance systems in public hospitals settings in Indonesia. The scale can be used as a feedback for first-line nurse managers to see the impact of competence-based human resource management framework on their competence and helps to identify which dimension of human resource management needs further improvement. With further research, the scale may be of use in other countries and settings.

15.
Environ Sci Technol ; 2021 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-33793220

RESUMEN

Nitrated phenols (NPs) are important atmospheric pollutants that affect air quality, radiation, and health. The recent development of the time-of-flight chemical ionization mass spectrometer (ToF-CIMS) allows quantitative online measurements of NPs for a better understanding of their sources and environmental impacts. Herein, we deployed nitrate ions as reagent ions in the ToF-CIMS and quantified six classes of gaseous NPs in Beijing. The concentrations of NPs are in the range of 1 to 520 ng m-3. Nitrophenol (NPh) has the greatest mean concentration. Dinitrophenol (DNP) shows the greatest haze-to-clean concentration ratio, which may be associated with aqueous production. The high concentrations and distinct diurnal profiles of NPs indicate a strong secondary formation to overweigh losses, driven by high emissions of precursors, strong oxidative capacity, and high NOx levels. The budget analysis on the basis of our measurements and box-model calculations suggest a minor role of the photolysis of NPs (<1 ppb h-1) in producing OH radicals. NPs therefore cannot explain the underestimated OH production in urban environments. Discrepancies between these results and the laboratory measurements of the NP photolysis rates indicate the need for further studies aimed at understanding the production and losses of NPs in polluted urban environments.

16.
Pediatr Res ; 2021 Mar 31.
Artículo en Inglés | MEDLINE | ID: mdl-33790413

RESUMEN

BACKGROUND: Lethal neonatal rigidity and multifocal seizure syndrome (RMFSL) is caused by variants in BRAT1 (BRCA1-associated protein required for ATM activation-1). However, the molecular mechanism of RMFSL is still unclear. METHODS: An RMFSL infant was recruited and the peripheral blood samples from his trio-family were collected. The genomic DNA was extracted, and then the whole-exome sequencing was performed. The expression of BRAT1 was analyzed by Western blotting. The subcellular localization of BRAT1 and MitoSOX (mitochondrial superoxide level) was investigated by confocal microscopy. The RNA samples were obtained from transfected cells, and then the RNA sequencing was performed. RESULTS: In this study, a novel homozygous BRAT1 variant c.233G > C with amino acid change of R with P at residue 78 (R78P) was identified. This variant altered the peptide structure and subcellular localization, as well as the expression in vitro. However, R78P did not alter the ability of BRAT1 to downregulate MitoSOX in mitochondria. Meanwhile, R78P BRAT1 was positively correlated with temporal lobe epilepsy, autosomal recessive primary microcephaly, defective/absent horizontal voluntary eye movements, and neuron apoptotic process as indicated by gene set enrichment analysis (GSEA). CONCLUSIONS: The BRAT1 variant spectrum has been expanded, which will be helpful for genetic counseling. We also explored the molecular mechanism altered by R78P, which will provide a better understanding of the pathogenesis of RMFSL. IMPACT: The detailed course of an infant with lethal neonatal RMFSL was depicted. A novel disease-causing variant R78P in BRAT1 for lethal neonatal RMFSL was identified. R78P led to reduced BRAT1 expression and nuclear localization in vitro. R78P did not alter the ability of BRAT1 to downregulate MitoSOX in the mitochondria. The variant R78P in BRAT1 was positively correlated with temporal lobe epilepsy, autosomal recessive primary microcephaly, defective/absent horizontal voluntary eye movements, and neuron apoptotic process as indicated by GSEA.

17.
Physiol Plant ; 2021 Mar 04.
Artículo en Inglés | MEDLINE | ID: mdl-33665820

RESUMEN

With the rapid development of facility agriculture, it has become popular to study the influences of different light qualities on the growth, material metabolism, and morphology of horticultural crops. Last several years, green onions cultivation models have undergone major changes, and facility cultivation has developed rapidly. To determine the impact of light quality on the green onions, we studied the parameters connected to photosynthesis, incorporating growth, and development, photosynthetic rate (Pn ), chlorophyll fluorescence, light response curve, photosynthetic electron transfer, and chloroplast ultrastructure. We roundly analyzed the influences of different LED light combination (white: W, white-blue combination 3:1:WB, white-green combination 3:1:WG, white-yellow combination 3:1:WY, and white-red combination 3:1:WR, light intensity: 500 ± 10 µmol photons m-2 s-1 ) on the photosynthetic performance of green onions. The WB light led to better results than those of the WR, WG, and WY. There were significant performance improvements in leaf area, plant height, stem thickness, relative growth rate (RGR), pigment content, photosynthetic capacity, photosynthetic electron transfer efficiency, and chloroplast ultrastructure integrity. In contrast, plants treated with WG and WY were exposed to appreciably blocked light, but they effectively formed a light protection mechanism. The results of this research not only provided insight into the response mechanism of crop photosynthesis to different light qualities, but they also provided a scientific foundation for better planting green onions.

18.
J Bioenerg Biomembr ; 53(2): 223-233, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33687619

RESUMEN

Non-small cell lung cancer (NSCLC) is a common cancer with an unfavorable 5-year survival rate. We intended to explore the role of circular RNA_0074027 (circ_0074027) in NSCLC progression. The levels of circ_0074027, messenger RNA (mRNA) of its linear form paired like homeodomain 1 (PITX1), microRNA-2467-3p (miR-2467-3p) and ras homolog family member A (RHOA) mRNA were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Cell Counting Kit-8 (CCK8) assay and plate colony assay were conducted to measure the proliferation ability of NSCLC cells. Transwell assays were used to assess cell migration and invasion abilities. Flow cytometry was utilized to analyze cell apoptosis rate. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were used to test the interaction between miR-2467-3p and circ_0074027 or RHOA. Western blot assay was performed to evaluate the protein level of RHOA in NSCLC cells. Murine xenograft model was built to evaluate the role of circ_0074027 in tumor growth in vivo. Circ_0074027 expression was prominently elevated in NSCLC tissues and cell lines. Circ_0074027 knockdown or miR-2467-3p overexpression suppressed cell proliferation, migration and invasion and facilitated cell apoptosis of NSCLC cells. Circ_0074027 interacted with miR-2467-3p, and RHOA was a target of miR-2467-3p in NSCLC cells. RHOA silencing blocked the malignant potential of NSCLC cells. Circ_0074027 silencing restrained the malignant phenotypes of NSCLC cells largely through up-regulating miR-2467-3p. Circ_0074027 knockdown notably blocked xenograft tumor growth in vivo. In conclusion, circ_0074027 accelerated NSCLC progression by binding to miR-2467-3p to induce RHOA expression.

19.
PLoS One ; 16(3): e0247682, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33780473

RESUMEN

With the development of a large number of tall dams and large cascade reservoir projects in the Lantsang River Basin, a large water level fluctuating zone (WLFZ) containing cascading reservoirs has formed. This newborn ecosystem is related to the sustainable development of hydropower projects, and has become a new problem to be studied urgently. Taking WLFZs in the Huangdeng, Xiaowan and Nuozhadu Reservoirs in the Lantsang River Basin as study areas, this study used multi-spectral remote-sensing field data obtained with unmanned aerial vehicles (UAVs) to ascertain the species types, coverage, distribution characteristics, dominant species and pioneer species of naturally restored vegetation. The considered data were subjected to UAV data processing, vegetation classification using multi-spectral images and a geographic information system (GIS) terrain-distribution analysis. Results show that: Polygonum Plebeium, Cynodon dactylon, Xanthium sibiricum, Ageratum conyzoides, Eleusine indica, Digitaria sanguinalis and Verbena officinalis are the dominant species of vegetation that could be naturally restored in the WLFZ; the vegetation coverage and the number of species are significantly positively correlated with the age and restoration periods of the WLFZ; the vegetation coverage of each study area increases at first, and then decreases, as a function of elevation; gentle slopes about 0-25°are more suitable for vegetation restoration. This study provides first-hand data on the natural restoration of vegetation in WLFZs, and gives a useful reference for its ecological restoration as a consequence of hydropower cascade development in the Lantsang River Basin. Finally, the study demonstrates that light UAV remote sensing is an attractive choice for investigating vegetation in reservoir WLFZs.

20.
Chin J Nat Med ; 19(3): 195-204, 2021 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33781453

RESUMEN

Paris saponin VII (PSVII), a bioactive constituent extracted from Trillium tschonoskii Maxim., is cytotoxic to several cancer types. This study was designed to explore whether PSVII prevents non-small-cell lung cancer (NSCLC) proliferation and to investigate its molecular target. AMP-activated protein kinase (AMPK) has been implicated in the activation of autophagy in distinct tissues. In cultured human NSCLC cell lines, PSVII induces autophagy by activating AMPK and inhibiting mTOR signaling. Furthermore, PSVII-induced autophagy activation was reversed by the AMPK inhibitor compound C. Computational docking analysis showed that PSVII directly interacted with the allosteric drug and metabolite site of AMPK to stabilize its activation. Microscale thermophoresis assay and drug affinity responsive target stability assay further confirmed the high affinity between PSVII and AMPK. In summary, PSVII acts as a direct AMPK activator to induce cell autophagy, which inhibits the growth of NSCLC cells. In the future, PSVII therapy should be applied to treat patients with NSCLC.

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