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1.
Biomed Pharmacother ; 134: 111149, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33385683

RESUMEN

E. coli is associated with high rates of infection and resistance to drugs not only in China but also the rest of the world. In addition, the number of E. coli biofilm infections continue to increase with time. Notably, biofilms are attractive targets for the prevention of infections caused by multidrug-resistant bacteria. Moreover, the pgaABCD-encoded Poly-ß-1,6-N-acetyl-d-glucosamine (PNAG) plays an important role in biofilm formation. Therefore, this study aimed to explore the specific effect of the (R)-(+)-pulegone (PU) on growth and biofilm formation in multi-drug resistant E. coli. The molecular mechanisms involved were also examined. The results showed that PU had significant antibacterial and antibiofilm formation activity against E. coli K1, with MIC and MBC values of 23.68 and 47.35 mg/mL, respectively. On the other hand, the maximum inhibition rate for biofilm formation in the bacterium was 52.36 % at 94.70 mg/mL of PU. qRT-PCR data showed that PU significantly down-regulated expression of the pgaABCD genes (P < 0.05). PU was also broadly effective against biofilm formation in MG1655 and MG1655/ΔpgaABCD, exhibiting the maximum inhibition rates were 98.23 % and 93.35 %, respectively. In addition, PU destroyed pre-formed mature biofilm in both MG1655 and MG1655/ΔpgaABCD about 95.03 % and 92.4 %, respectively. The study therefore verified that pgaA was a potential and key target for PU in E. coli although it was not the only one. Overall, the findings indicated that PU is a potential and novel inhibitor of drug resistance, This therefore gives insights on new ways of preventing and treating biofilm-associated infections in the food industry as well as in clinical practice.


Asunto(s)
Antibacterianos/farmacología , Proteínas de la Membrana Bacteriana Externa/genética , Biopelículas/efectos de los fármacos , Monoterpenos Ciclohexánicos/farmacología , Farmacorresistencia Bacteriana Múltiple/genética , Escherichia coli K12/efectos de los fármacos , Proteínas de Escherichia coli/genética , Amidohidrolasas/genética , Biopelículas/crecimiento & desarrollo , Escherichia coli K12/genética , Escherichia coli K12/crecimiento & desarrollo , Regulación Bacteriana de la Expresión Génica , Pruebas de Sensibilidad Microbiana
2.
Biomed Pharmacother ; 131: 110684, 2020 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-33152903

RESUMEN

Marein, an active component of the Coreopsis tinctoria Nutt. plant, is known to improve diabetic nephropathy (DN). However, its anti-diabetic functions in DN and potential mechanisms remain unclear. The aim of this study was to elucidate the effects and mechanisms of Marein in diabetic db/db mice with DN, and in high glucose-treated HK-2 cells. In vivo, treating diabetic db/db mice with Marein for 12 consecutive weeks restored diabetes-induced hyperglycemia and dyslipidemia, and ameliorated renal function deterioration, glomerulosclerosis, and renal ectopic lipid deposition. Marein exerted renoprotective effects by directly inhibiting renal tubule sodium glucose transporter 2 (SGLT2) expression, and then activating the AMP-activated protein kinase (AMPK)/acetyl CoA carboxylase (ACC)/peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α) pathway in db/db mice. Meanwhile, Marein ameliorated fibrosis and inflammation by suppressing the pro-inflammatory factors interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1), and expression of the extracellular matrix proteins, fibronectin (FN) and collagen 1 (COL1) in diabetic mice. In vitro, MDCK monolayer cells were established to explore the characteristics of Marein transmembrane transport. Marein was found to be absorbed across the membrane at a medium level that involved active transport and this was mediated by SGLTs. In HK-2 cells, Marein decreased uptake of the fluorescent glucose analog, 2-NBDG, by 22 % by inhibiting SGLT2 expression. In high glucose-treated HK-2 cells, Marein decreased SGLT2 expression and increased phosphorylated (p)-AMPK/p-ACC to improve high glucose-induced cellular dysfunction. Furthermore, Marein treatment decreased SGLT2 expression in SGLT2-overexpressing HK-2 cells. In addition, molecular docking and dynamics analysis revealed that SGLT2 was a direct target of Marein. Collectively, our results demonstrated that Marein ameliorates DN by inhibiting renal SGLT2 and activating p-AMPK, suggesting Marein can potentially prevent DN by suppressing renal SGLT2 expression directly.

3.
Opt Express ; 28(10): 15081-15089, 2020 May 11.
Artículo en Inglés | MEDLINE | ID: mdl-32403541

RESUMEN

An all-optical self-oscillating 4He atomic magnetometer with a large dynamic range of the magnetic field is demonstrated. This device has the advantage of the fast response of the self-oscillating magnetometer and is not affected by the systematic errors originated from the radio-frequency field. It is also free from the nonlinear Zeeman effect in large magnetic fields. We use a liquid crystal to adjust the phase shift, which is independent of frequency. Results show that our self-oscillating 4He magnetometer exhibits a response time of 0.2 ms for a step signal of 3600 nT, and the noise floor reaches 1.7 pT / Hz1/2 for frequencies from 2 Hz to 500 Hz. It can work stably in magnetic fields ranging from 2500 nT to 103000 nT. Compared with the commercial self-oscillating cesium atomic magnetometer (Scintrex, CS-3), the self-oscillating 4He atomic magnetometer has shown a better gradient tolerance in larger magnetic field. This magnetometer is ideally suited in magnetic observatories to monitor geomagnetic field requiring large dynamic range and high bandwidth.

4.
Sci Rep ; 10(1): 439, 2020 01 16.
Artículo en Inglés | MEDLINE | ID: mdl-31949205

RESUMEN

Flavanomarein (FM) is a major natural compound of Coreopsis tinctoria Nutt with protective effects against diabetic nephropathy (DN). In this study, we investigated the effects of FM on epithelial-mesenchymal transition (EMT) in high glucose (HG)-stimulated human proximal tubular epithelial cells (HK-2) and the underlying mechanisms, including both direct targets and downstream signal-related proteins. The influence of FM on EMT marker proteins was evaluated via western blot. Potential target proteins of FM were searched using Discovery Studio 2017 R2. Gene Ontology (GO) analysis was conducted to enrich the proteins within the protein-protein interaction (PPI) network for biological processes. Specific binding of FM to target proteins was examined via molecular dynamics and surface plasmon resonance analyses (SPR). FM promoted the proliferation of HK-2 cells stimulated with HG and inhibited EMT through the Syk/TGF-ß1/Smad signaling pathway. Spleen tyrosine kinase (Syk) was predicted to be the most likely directly interacting protein with FM. Combined therapy with a Syk inhibitor and FM presents significant potential as an effective novel therapeutic strategy for DN.


Asunto(s)
Transición Epitelial-Mesenquimal/efectos de los fármacos , Glucosa/farmacología , Quinasa Syk/metabolismo , Actinas/metabolismo , Cadherinas/metabolismo , Línea Celular , Proliferación Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Fibronectinas/metabolismo , Regulación de la Expresión Génica/efectos de los fármacos , Humanos , Riñón/citología , Simulación del Acoplamiento Molecular , Conformación Proteica , Transducción de Señal/efectos de los fármacos , Proteínas Smad/metabolismo , Quinasa Syk/química , Factor de Crecimiento Transformador beta1/metabolismo , Vimentina/metabolismo
5.
J Ethnopharmacol ; 250: 112479, 2020 Mar 25.
Artículo en Inglés | MEDLINE | ID: mdl-31846746

RESUMEN

ETHNOPHARMACOLOGICAL RELEVANCE: Urolithin A (UroA), the main intestinal microflora metabolite of ellagic acid of berries, pomegranate,and some other traditional chinese herbals such as emblica officinalis,etc,has been reported to exhibit anti-inflammatory, anti-oxidative, anti-tumor and pro-autophagy effects. AIM OF THE STUDY: This study evaluated the anti-diabetic and pancreas-protective effects of UroA using a mice model of type 2 diabetes and preliminarily explored its effect on autophagy as well as the mechanism involved. MATERIALS AND METHODS: Type 2 diabetes model was induced by high-fat diet (HFD; 60% energy as fat) and low-dose streptozotocin (85 mg/kg) injection. Mice were administered with UroA (50 mg/kg/d) alone or UroA-chloroquine (autophagy inhibitor) combination for 8 weeks. RESULTS: UroA improved symptoms of diabetic mice such as high water intake volume, high urine volume, significantly decreased fasting blood glucose (FBG), after-glucose-loading glucose, glycated hemoglobin (GHb) levels, plasma C-peptide, malondialdehyde (MDA) and interleukin-1 ß level, increased reduced glutathione (GSH), interleukin-10 content, and glucose tolerance. UroA also improved pancreatic function indexes such as HOMA-ß as evidenced by improved pathological and ultrastructural features of the pancreas assessed by light microscopy and transmission electron microscopy (TEM). Accordingly, UroA decreased mitochondrial swelling and myelin-like cytoplasmic inclusions. UroA significantly upregulated the protein levels of microtubule-associated protein 1 light chain 3-II (LC3II) and beclin1, downregulated sequestosome 1 (p62) accompanied by decreased expression of apoptotic protein cleaved caspase3 in pancreas of diabetic mice. In addition, it increased the phosphorylation level of protein kinase B (p-Akt) and mammalian target of rapamycin (p-mTOR). Most of these effects of UroA were reversed by treatment with autophagy inhibitor chloroquine. CONCLUSIONS: Our findings reveal that the pancreas protective effects of UroA against diabetes were partially mediated by its regulation of autophagy and AKT/mTOR signal pathway.


Asunto(s)
Cumarinas/farmacología , Diabetes Mellitus Experimental/tratamiento farmacológico , Diabetes Mellitus Tipo 2/tratamiento farmacológico , Páncreas/efectos de los fármacos , Animales , Autofagia/efectos de los fármacos , Glucemia/efectos de los fármacos , Cloroquina/farmacología , Diabetes Mellitus Experimental/fisiopatología , Diabetes Mellitus Tipo 2/fisiopatología , Dieta Alta en Grasa/efectos adversos , Masculino , Ratones , Ratones Endogámicos C57BL , Páncreas/patología , Proteínas Proto-Oncogénicas c-akt/metabolismo , Transducción de Señal/efectos de los fármacos , Estreptozocina , Serina-Treonina Quinasas TOR/metabolismo
6.
Biomed Res Int ; 2019: 6159793, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31346521

RESUMEN

Objective: The use of cumulative live birth rate (CLBR) per ovarian stimulation cycle is proving to be an accurate method to calculate the success of IVF; however, the cycle outcome is closely associated with the number of embryos transferred (ET). Our aim was to report CLBR after IVF according to the number of embryos required to achieve a live birth in women aged ≥35 years, considering age, body mass index (BMI), and ethnicity. Methods: We conducted a retrospective cohort study including 1344 patients who underwent IVF between January 2013 and June 2016 at the First Affiliated Hospital of Xinjiang Medical University. The cumulative probability of live birth for each couple was estimated using the Kaplan-Meier method, and survival curves were compared according to age, BMI, and ethnicity using the log-rank test. Results: CLBR increased rapidly from 1 to 5 ETs, moderately from 6 to 10 ETs, and slowly thereafter. CLBR was significantly different across 4 categories based on BMI as well as across those based on age; low CLBR was significantly associated with the age of ≥42 years and obesity. Conclusion: The association between CLBR and number of ET provides realistic and precise information regarding the success of IVF and can be applied to guide clinicians and patients.


Asunto(s)
Transferencia de Embrión , Fertilización In Vitro , Nacimiento Vivo , Inducción de la Ovulación , Adulto , Factores de Edad , Femenino , Humanos , Persona de Mediana Edad , Estudios Retrospectivos
7.
Biomed Res Int ; 2019: 5280514, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31032350

RESUMEN

The study aims to investigate the effects of the alcohol extract of Coreopsis tinctoria Nutt (AC) on diabetic nephropathy (DN) mice. A total of 30 db/db (DN) mice were divided into 3 groups, which were treated with AC (300 mg/kg/day), metformin (180 mg/kg/day), or saline by gavage for 10 weeks. Ten db/m mice treated with saline were used as normal control (NC group). Body weight (BW) and fasting blood glucose (FBG), HbA1c, 24 h urinary albumin excretion (UAE), and renal pathological fibrosis were analyzed. Expression of miR-192, miR-200b, and proteins in the PTEN/PI3K/AKT pathway was analyzed by qPCR or western blot. The DN mice had significantly higher BW, FBG, and 24 h UAE, as well as more severe pathological fibrosis when compared with NC. Treatment of AC could decrease BW, FBG, and 24 h UAE and alleviated kidney damage. Compared with the NC group, expressions of miR-192 and miR-200b were increased, whereas their target proteins (ZEB2 and PTEN) were reduced in the kidneys of DN mice, which further modulated the expression of their downstream proteins PI3K p85α, P-AKT, P-smad3, and COL4 α1; these proteins were increased in the kidneys of DN mice. In contrast, AC treatment reversed the expression changes of these proteins. These findings demonstrate that AC may protect the kidneys of DN mice by decreasing miR-192 and miR-200b, which could further regulate their target gene expression and modulate the activity of the PTEN/PI3K/AKT pathway to reduce the degree of renal fibrosis.


Asunto(s)
Coreopsis/química , Diabetes Mellitus/tratamiento farmacológico , Nefropatías Diabéticas/tratamiento farmacológico , MicroARNs/genética , Albuminuria/orina , Alcoholes/química , Animales , Glucemia/aislamiento & purificación , Diabetes Mellitus/genética , Diabetes Mellitus/patología , Diabetes Mellitus/orina , Nefropatías Diabéticas/genética , Nefropatías Diabéticas/patología , Nefropatías Diabéticas/orina , Modelos Animales de Enfermedad , Regulación de la Expresión Génica/efectos de los fármacos , Hemoglobina A Glucada/aislamiento & purificación , Humanos , Riñón/efectos de los fármacos , Riñón/fisiología , Ratones , Ratones Endogámicos NOD , Fosfohidrolasa PTEN/genética , Extractos Vegetales/administración & dosificación , Extractos Vegetales/química , Proteínas Proto-Oncogénicas c-akt/genética , Transducción de Señal/efectos de los fármacos , Caja Homeótica 2 de Unión a E-Box con Dedos de Zinc/genética
8.
BMC Complement Altern Med ; 19(1): 14, 2019 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-30630477

RESUMEN

BACKGROUND: Coreopsis tinctoria Nutt is an ethnomedicine widely used in Xinjiang, China. It is consumed as a herbal tea by local Uyghur people to treat high blood pressure and diarrhea. Our previous study confirmed that the ethyl acetate extract of Coreopsis tinctoria (AC) had a protective effect on diabetic nephropathy (DN) in an in vivo experiment. Here we aim to elucidate the protective mechanism of AC and marein, the main ingredient in Coreopsis tinctoria on renal fibrosis and inflammation in vitro under high glucose (HG) conditions. METHODS: A HG-induced barrier dysfunction model in rat mesangial cells (HBZY-1) was established. The cells were exposed to AC and marein and/or HG for 24 h. Then, the renal protective effects of AC and marein via transforming growth factor-ß1 (TGF-ß1)/Smads, AMP-activated kinase protein (AMPK), and nuclear factor kappa beta (NF-κB) signaling were assessed. RESULTS: Both AC and marein suppressed rat mesangial cell hyperplasia and significantly attenuated the expression of HG-disrupted fibrotic and inflammatory proteins in HBZY-1 cells. It was also confirmed that AC and marein remarkably attenuated HG-induced renal inflammation and fibrosis by regulating the AMPK, TGF-ß1/Smads, and NF-κB signaling pathways. CONCLUSION: These results indicated that AC and marein may delay the progression of DN, at least in part, by suppressing HG-induced renal inflammation and fibrosis. Marein may be one of the bioactive compounds in AC.


Asunto(s)
Coreopsis/química , Medicamentos Herbarios Chinos/farmacología , Glucosa/efectos adversos , Enfermedades Renales/inmunología , FN-kappa B/inmunología , Proteínas Quinasas/inmunología , Proteínas Smad/inmunología , Factor de Crecimiento Transformador beta1/inmunología , Animales , Chalconas/farmacología , Fibrosis/tratamiento farmacológico , Fibrosis/genética , Fibrosis/inmunología , Humanos , Enfermedades Renales/inducido químicamente , Enfermedades Renales/tratamiento farmacológico , Células Mesangiales/efectos de los fármacos , Células Mesangiales/inmunología , FN-kappa B/genética , Proteínas Quinasas/genética , Ratas , Transducción de Señal/efectos de los fármacos , Proteínas Smad/genética , Factor de Crecimiento Transformador beta1/genética
9.
PeerJ ; 6: e4335, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29404223

RESUMEN

Background: Studies on postpartum depression (PPD) in China have focused primarily on women of Han ethnicity, whereas work on other ethnic groups has proven limited. This study explored the ethnic differences of associated social-demographic and obstetric factors for PPD between Han-majority and Kazak-minority women in northwestern China. Methods: Han and Kazak women who received routine examinations at four hospitals in a multi-ethnic area of China six weeks after childbirth between March 2016 and December 2016 were included in the study. Data on the women's socio-demographic characteristics, obstetric factors, and possible depression at six weeks after childbirth were collected. We examined the associated factors of PPD using multivariable logistic regression analyses by ethnic group. Results: The overall incidence of PPD was 14.6% (184/1,263) at six weeks after childbirth. PPD was detected more frequently among Kazak (16.1%) than Han women (13.1%). Kazak women exhibited a higher risk of PPD (adjusted OR = 1.561, 95% CI [1.108-2.198], P = 0.011). Urinary incontinence (UI) represented a significant risk factor of PPD for Kazak compared with Han women (OR = 1.720, 95% CI [1.056-2.804], P = 0.003). In contrast, the presence of the mother-in-law as a caregiver after childbirth demonstrated a positive association with PPD among Han (OR = 2.600, 95% CI [1.499-4.512], P = 0.001), but not with Kazak women. Conclusions: Kazak women were more likely to develop PPD than Han women, even after controlling for confounders. Moreover, distinct risk factors for PPD existed for Han and Kazak women. Future research that explores the relationships between Han women and their mothers-in-law as well as Kazak women's attitudes toward UI could help us further understand PPD in these populations.

10.
Exp Clin Endocrinol Diabetes ; 126(10): 604-611, 2018 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-29117617

RESUMEN

Free fatty acids (FFAs) participate in a variety of physiological functions. FFAs are associated with the development of type 2 diabetes mellitus (T2DM). Uyghurs and Kazaks have a different prevalence of T2DM, which cannot be explained by traditional risk factors. This study aimed to examine FFAs as potential biomarkers to distinguish between healthy and T2DM Uyghurs and Kazaks. This was a prospective study conducted at the Xianjiang Medical University from 01/2007 to 06/2010 in Uyghurs and Kazaks. The subjects were grouped as T2DM patients (Uyghurs: n=39; Kazaks, n=21) and controls (Uyghurs: n=35; Kazaks, n=40). Gas chromatography-mass spectrometry (GC-MS) and partial least squares discriminant analysis (PLS-DA) models were used to study the FFA profiles between Uyghurs and Kazaks with T2DM. PLS-DA analysis showed that among Kazaks, T2DM patients had lower C22:6, C18:3 n-6, and C20:3 n-6, but higher C22:0 levels compared with controls. Among Uyghurs, the most important variables to discriminate T2DM patients from controls were higher C22:6 and C20:4 n-6, and lower C22:0, C14:1, C18:3 n6, and C20:3 n6. Kazaks and Uyghurs displayed different FFA profiles between patients with T2DM and controls. These results suggest different risk factors and pathogenesis of T2DM between Kazaks and Uyghurs.


Asunto(s)
Diabetes Mellitus Tipo 2 , Ácidos Grasos no Esterificados/sangre , Modelos Biológicos , Adulto , Anciano , Biomarcadores , China/epidemiología , China/etnología , Cromatografía , Diabetes Mellitus Tipo 2/sangre , Diabetes Mellitus Tipo 2/epidemiología , Diabetes Mellitus Tipo 2/etnología , Femenino , Humanos , Masculino , Espectrometría de Masas , Persona de Mediana Edad , Factores de Riesgo
11.
Zhongguo Zhong Yao Za Zhi ; 42(4): 772-776, 2017 Feb.
Artículo en Chino | MEDLINE | ID: mdl-28959851

RESUMEN

To compare the amino acid metabolic profiling in urine of spontaneously hypertensive rats (SHR) and normal Wistar rats, and investigate the regulatory effect of extract from Coreopsis tinctoria on blood pressure and amino acid metabolic profiling in SHR. Right aged SHR and Wistar rats were housed to fit the new environment for 2 weeks. After that, their systolic pressure(SBP), diastolic pressure(DBP) were measured and urine was collected. Amino acids profiles for SHR and Wistar rats were acquired by using AQC precolumn derivatization HPLC-fluorescence method, and then partial least squares discriminant analysis(PLS-DA) was applied to facilitate differentiation and determine metabolic differences between collected samples from two groups of rats. Consequently, 40 SHR were randomly divided into 5 groups: model group, high, middle, low dosage groups of C. tinctoria extract (3.2, 1.6,0.8 g•kg⁻¹), and captopril group (4 mg•kg⁻¹). They were treated for 4 weeks by ig administration, and then their urine samples were collected to determine the amino acid metabolic profiling in various groups. After treatment for 4 weeks, as compared with Wistar group, serine, alanine, tyrosine, and cystine in the amino acid metabolic profiling were significantly increased in SHR group. As compared with SHR model group, threonine and methionine were decreased significantly in captopril group (P<0.01); amino acid metabolism was changed to different degrees in high, middle, and low dosage groups of C. tinctoria extract, and the threonine in low dose group was significantly decreased (P<0.01); serine and threonine were decreased (P<0.05), and valine, methionine and lysine were significantly decreased (P<0.01) in middle dose group; threonine, valine, methionine and lysine were significantly decreased in large dose group (P<0.01). The results showed that middle and high doses of extract from C. tinctoria could significantly improve disturbance of amino acid metabolism, help to further clarify the drug property research of C. tinctoria, and provide data support for amino acid metabolic pathway abnormalities in hypertension patients.


Asunto(s)
Aminoácidos/metabolismo , Presión Sanguínea/efectos de los fármacos , Coreopsis/química , Extractos Vegetales/farmacología , Animales , Hipertensión , Metaboloma , Ratas , Ratas Endogámicas SHR , Ratas Wistar
12.
PLoS One ; 12(3): e0172774, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28328990

RESUMEN

The gut microbiome may have an important influence on the development of diabetes mellitus type 2 (DM2). To better understand the DM2 pandemic in ethnic minority groups in China, we investigated and compared the composition and richness of the gut microbiota of healthy, normal glucose tolerant (NGT) individuals and DM2 patients from two ethnic minority groups in Xinjiang, northwest China, the Uygurs and Kazaks. The conserved V6 region of the 16S rRNA gene was amplified by PCR from the isolated DNA. The amplified DNA was sequenced and analyzed. An average of 4047 high quality reads of unique tag sequences were obtained from the 40 Uygurs and Kazaks. The 3 most dominant bacterial families among all participants, both healthy and DM2 patients, were the Ruminococcaceae, Lachnospiraceae, and Enterobacteriaceae. Significant differences in intestinal microbiota were found between the NGT individuals and DM2 patients, as well as between the two ethnic groups. Our findings shed new light on the gut microbiome in relation to DM2. The differentiated microbiota data may be used for potential biomarkers for DM2 diagnosis and prevention.


Asunto(s)
Diabetes Mellitus Tipo 2/microbiología , Microbioma Gastrointestinal/genética , Adulto , Grupo de Ascendencia Continental Asiática , China , ADN Bacteriano/genética , Grupos Étnicos , Heces/microbiología , Humanos , Microbiota/genética , Persona de Mediana Edad , Grupos Minoritarios , ARN Ribosómico 16S/genética
13.
J Ethnopharmacol ; 186: 73-83, 2016 Jun 20.
Artículo en Inglés | MEDLINE | ID: mdl-27045866

RESUMEN

ETHNOPHARMACOLOGICAL RELEVANCE: Coreopsis tinctoria Nutt mainly distributed in Hetian region of Xinjiang at an altitude of 3000m, which is used as Uyghur traditional medicine because of its clearing heat, promoting circulation and removing toxicity and antihypertension, ect. effect. AIM OF THE STUDY: This research was to study the four ingredients in the extracts of Coreopsis tinctoria Nutt. that are absorbed in different intestinal segments in rats to lay the foundation for further study on the effective constituents, tissue distribution, metabolism, and spectrum-effect relationships of these extracts. MATERIALS AND METHODS: High, medium, and low concentrations were prepared according to their pharmacological effects. Quantitative analysis multi-components by single marker was used to test the cumulative absorption volume Q, absorption rate constant Ka, and apparent permeability coefficient Papp of the four main ingredients in C. tinctoria Nutt. extract in different intestinal segments in rats using a Ussing chamber model and high-performance liquid chromatography. RESULTS: The Papp of chlorogenic acid and flavanomarein in the duodenum, jejunum, ileum, and colon were 1.0×10(-6) to 10×10(-6)cms(-1). Papp of marein in the duodenum and jejunum was <1.0×10(-6), and was 1.0×10(-6) to 10×10(-6)cms(-1) in the ileum and colon. Papp of 3,5-O-dicaffeoylquinic acid in the duodenum was <1.0×10(-6)cms(-1), while it was 1.0×(1)0(-6) to 10×10(-6)cms(-1) in the jejunum, ileum, and colon. CONCLUSIONS: All four chemical components of the plant extract can be absorbed by the intestinal canal of rats, which conforms to zero-order absorption; the ileum presented the best absorption.


Asunto(s)
Coreopsis/química , Mucosa Intestinal/metabolismo , Extractos Vegetales/farmacocinética , Animales , Límite de Detección , Permeabilidad , Extractos Vegetales/química , Ratas , Reproducibilidad de los Resultados
14.
Zhongguo Zhong Yao Za Zhi ; 41(22): 4226-4233, 2016 Nov.
Artículo en Chino | MEDLINE | ID: mdl-28933093

RESUMEN

To study the effect of plant protein and animal protein on amino acid metabolism spectrum of Qi and Yin deficiency type 2 diabetic rats. 110 male SD rats were randomly divided into blank group (n=10), diabetic model group (n=20), disease-symptoms group (n=80). The rats of blank group received ordinary feeding, while other groups were fed with high sugar and fat diets. During the whole process of feeding, rats of disease-symptoms group were given with Qingpi-Fuzi (15.75 g•kg⁻¹) once a day through oral administration. Five weeks later, the rats were given with a low dose of STZ (40 mg•kg⁻¹) by intraperitoneal injection to establish experimental diabetic models. Then the models were randomly divided into disease-symptoms group 1 (Qi and Yin deficiency diabetic group, 15.75 g•kg⁻¹), disease-symptoms group 2 (plant protein group, 0.5 g•kg⁻¹), disease-symptoms group 3 (animal protein group, 0.5 g•kg⁻¹), disease-symptoms group 4 (berberine group, 0.1 g•kg⁻¹). The drugs were given for 4 weeks by gavage administration. After 4 weeks of protein intervention, the abdominal aortic blood was collected and serum was isolated to analyze its free amino acid by using AQC pre-column derivatization HPLC and fluorescence detector. Four weeks after the protein intervention, plant protein, animal protein and berberine had no obvious effect on body weight and blood sugar in type 2 diabetic rats. As compared with animal protein group, histidine and proline(P<0.01), serine, glycine, threonine, alanine, tyrosine, valine, methionine, bright+isoleucine, phenylalanine and lysine(P<0.05)changed a lot in rats serum of plant protein group.The results showed that gavage administration of protein would produce effects on amino acid metabolism of Qi and Yin deficiency type 2 diabetic SD rats. Symbolic differential compounds could be found through metabonomics technology, providing experimental basis for early warning of type 2 diabetes and diagnosis of Qi and Yin deficiency syndrome.


Asunto(s)
Aminoácidos/metabolismo , Diabetes Mellitus Experimental/tratamiento farmacológico , Dieta , Medicamentos Herbarios Chinos/farmacología , Deficiencia Yin/tratamiento farmacológico , Animales , Diabetes Mellitus Tipo 2/tratamiento farmacológico , Masculino , Medicina China Tradicional , Qi , Ratas , Ratas Sprague-Dawley
15.
Am J Transl Res ; 7(10): 1984-91, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26692941

RESUMEN

AIMS: MicroRNAs play important roles in energy metabolism, insulin synthesis, insulin transport and the development of diabetes. This study aims to investigate the expression and effect of microRNA-130a in Uygur patients with type 2 diabetes mellitus (T2DM). MATERIALS AND METHODS: Peripheral blood and omental adipose tissues were collected from individuals with normal glucose tolerance and patients with T2DM. The microRNA expression profile of peripheral blood was established by microarray analysis. The differentially expressed microRNAs and possible target genes were identified by bioinformatics analysis. MicroRNA-130a mimics and inhibitors were transfected into 3T3-L1 preadipocytes. RESULTS: Our results showed that microRNA-130a expression level was significantly decreased in peripheral blood and omental adipose tissues of T2DM patients (P < 0.01). Peroxisome proliferator-activated receptors γ (PPARγ) were predicted as target genes of microRNA-130a. This prediction was verified by the results that PPARγ mRNA expression in omental adipose tissues of T2DM patients were significantly increased (P < 0.01). The glucose consumption level after microRNA-130a transfection was significantly decreased (P < 0.05). And, microRNA-130a mimics inhibited PPARγ expression at both mRNA and protein level, further suggesting that PPARγ is a target gene of microRNA-130a. Additionally, adiponectin, lipoprotein lipase, CCAAT enhancer binding protein α, and the downstream genes of PPARγ, were significantly decreased after microRNA-130a mimics transfection. CONCLUSIONS: In conclusion, microRNA-130a is decreased in Uygur patients with T2DM and it may play a role in T2DM through targeting PPARγ.

16.
BMC Complement Altern Med ; 15: 314, 2015 Sep 07.
Artículo en Inglés | MEDLINE | ID: mdl-26346939

RESUMEN

BACKGROUND: Diabetic nephropathy is a serious complication of diabetes whose development process is associated with inflammation, renal hypertrophy, and fibrosis. Coreopsis tinctoria Nutt, traditionally used as a healthcare tea, has anti-inflammatory, anti-hyperlipidemia, and glycemic regulation activities. The aim of our study was to investigate the renal protective effect of ethyl acetate extract of C. tinctoria Nutt (AC) on high-glucose-fat diet and streptozotocin (STZ)-induced diabetic rats. METHODS: A diabetic rat model was induced by high-glucose-fat diet and intraperitoneal injection of 35 mg/kg STZ. After treatment with AC at a daily dose of 150, 300 or, 600 mg/kg for 4 weeks, metabolic and renal function parameters of serum and urine were examined. Degree of renal damage, renal proinflammatory cytokines, and fibrotic protein expression were analyzed by histopathology and immunohistochemistry. Renal AMP-activated protein kinase (AMPK) and transforming growth factor (TGF)-ß1/Smad signaling pathway were determined by western blotting. RESULTS: Diabetic rats showed obvious renal dysfunction, inflammation and fibrosis. However, AC significantly reduced levels of blood glucose, total cholesterol, triglyceride, blood urea nitrogen, serum creatinine and urinary albumin, as well as expression of kidney proinflammatory cytokines of monocyte chemoattractant protein-1 and intercellular adhesion molecule-1. AC also ameliorated renal hypertrophy and fibrosis by reducing fibronectin and collagen IV and suppressing the TGF-ß1/Smad signaling pathway. Meanwhile, AMPKα as a protective cytokine was markedly stimulated by AC. CONCLUSION: In summary, AC controls blood glucose, inhibits inflammatory and fibrotic processes, suppresses the TGF-ß1/Smad signaling pathway, and activates phosphorylation of AMPKα in the kidneys, which confirms the protective effects of AC in the early stage of diabetic kidney disease.


Asunto(s)
Antiinflamatorios/farmacología , Glucemia/efectos de los fármacos , Coreopsis/química , Diabetes Mellitus Experimental/metabolismo , Dieta Alta en Grasa , Riñón/efectos de los fármacos , Extractos Vegetales/farmacología , Animales , Ratas , Estreptozocina
17.
Zhongguo Zhong Yao Za Zhi ; 40(19): 3737-41, 2015 Oct.
Artículo en Chino | MEDLINE | ID: mdl-26975094

RESUMEN

With the application of monoclonal antibody technology more and more widely, its production technology is becoming more and more perfect. Small molecule monoclonal antibody technology is becoming a hot research topic for people. The application of traditional Chinese medicine small molecule monoclonal antibody technology has been more and more widely, the technology for effective Chinese medicine component knockout provide strong technical support. The preparation of monoclonal antibodies and small molecule knockout technology are reviewed in this paper. The preparation of several steps, such as: in the process of preparation of antigen, hapten carrier coupling, coupling ratio determination and identification of artificial antigen and establishment of animal immunization and hybridoma cell lines of monoclonal antibody, the large-scale preparation; small molecule monoclonal antibody on Immune in affinity chromatography column method is discussed in detail. The author believes that this technology will make the traditional Chinese medicine research on a higher level, and improve the level of internationalization of Chinese medicine research.


Asunto(s)
Anticuerpos Monoclonales/inmunología , Técnicas Inmunológicas/métodos , Animales , Anticuerpos Monoclonales/química , Anticuerpos Monoclonales/genética , Humanos , Hibridomas/metabolismo , Técnicas Inmunológicas/tendencias
18.
Diagn Pathol ; 9: 83, 2014 Apr 16.
Artículo en Inglés | MEDLINE | ID: mdl-24739504

RESUMEN

OBJECTIVE: This study is to determine if Adenovirus type 36 (Ad36) infection is related to macrophage infiltration in the obese group and non-obese group and the related molecular mechanisms. METHODS: Ninety obesity patients and 95 non-obesity Uygur individuals were enrolled in this study. CD68 levels in abdominal subcutaneous and omental adipose tissues were detected by immunohistochemistry. The cytokine expression levels of adiponectin (APMI) and visfatin in serum were measured by enzyme-linked immunosorbent assay. Infection of 3T3-L1 cells with Ad36 was performed. Real-time PCR was performed to determine expression levels of APMI and Visfatin genes in the 3T3-L1 preadipocytes infected with Ad36. RESULTS: In the obese individuals infected with Ad36, the expression levels of adiponectin and visfatin in serum was elevated. For the individuals infected with Ad36, the macrophage infiltration (as indicated by CD68 level) in the obese group was also significantly higher than that in the non-obese group (P < 0.05) in both abdominal subcutaneous and omental adipose tissues. The real-time PCR results indicated that APMI mRNA levels and Visfatin mRNA levels in Ad36 infected cells were significantly increased. CONCLUSIONS: Ad36 infection may be a factor related with macrophage infiltration in adipose tissues of the obese patients. The APMI and Visfatin genes may be involved in the mechanism underlying the effect of Ad36 infection on the obese patients. VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1849614638119816.


Asunto(s)
Infecciones por Adenovirus Humanos/sangre , Adiponectina/sangre , Citocinas/sangre , Nicotinamida Fosforribosiltransferasa/sangre , Sobrepeso/sangre , Células 3T3-L1 , Grasa Abdominal/metabolismo , Infecciones por Adenovirus Humanos/epidemiología , Infecciones por Adenovirus Humanos/genética , Adipocitos/metabolismo , Adipocitos/virología , Adiponectina/genética , Adiponectina/metabolismo , Adulto , Anciano , Animales , Antígenos CD/metabolismo , Antígenos de Diferenciación Mielomonocítica/metabolismo , Estudios de Casos y Controles , China/epidemiología , Citocinas/genética , Citocinas/metabolismo , Regulación de la Expresión Génica , Humanos , Macrófagos/metabolismo , Ratones , Persona de Mediana Edad , Nicotinamida Fosforribosiltransferasa/genética , Nicotinamida Fosforribosiltransferasa/metabolismo , Sobrepeso/epidemiología , Sobrepeso/genética , Factores de Tiempo
19.
Artículo en Inglés | MEDLINE | ID: mdl-24302962

RESUMEN

Ellagic acid (EA) present in many fruits and nuts serves as antiproliferation, anti-inflammatory, and antitumorigenic properties. However, the effect of EA on preadipocytes adipogenesis and its mechanism(s) have not been elucidated. The present study was designed to examine the effect of EA on adipogenesis in 3T3-L1 preadipocytes and underlying mechanism(s) of action involved. Data show that EA administration decreased the accumulation of lipid droplets. The inhibition was diminished when the addition of EA was delayed to days 2-4 of differentiation. Clonal expansion was reduced in the presence of EA. FACS analysis showed that EA blocked the cell cycle at the G1/S transition. EdU incorporation also confirmed that EA refrained cell from entering S phase. Our data also revealed that the differentiation-induced protein expression of Cyclin A and phosphorylation of the retinoblastoma protein (Rb) were impaired by EA. Differentiation-dependent expression and DNA-binding ability of C/EBP α were also inhibited by EA. Alterations in cell cycle-associated proteins may be important with respect to the antiadipogenic action of EA. In conclusion, EA is capable of inhibiting adipogenesis in 3T3-L1 adipocytes possibly through reduction of Cyclin A protein expression and Rb phosphorylation. With the blocking of G1/S phase transition, EA suppresses terminal differentiation and lipid accumulation in 3T3-L1 adipocytes.

20.
Exp Ther Med ; 6(3): 635-640, 2013 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-24137239

RESUMEN

Insulin resistance in obesity is associated with chronic systemic low-grade inflammation. Although it has been shown that Toll-like receptor 4 (TLR4) in the liver, muscle and adipose tissue plays an important role in obesity-associated inflammation and insulin resistance, the effect of TLR4 activation in the intestine has not been investigated. The aim of this study was to explore the activation of the mouse intestinal TLR4/NF-κB signaling pathway following the administration of a short-term high-fat diet, as well as the function of the signaling pathway in the local enteric inflammatory response. The effect of the high-fat diet on TLR4 activation, NF-κB and phosphorylated IκB (PIκB) activity, and tumor necrosis factor (TNF)-α and IL-6 expression in the intestinal tissues of diet-induced obese C57BL/6 mice was investigated. The results demonstrated that the high-fat diet induced TLR4 mRNA and protein expression in intestinal tissues. TLR4/NF-κB signaling pathway activation gradually increased as the number of days of high-fat diet administration increased, and peaked on day 7. Additionally, activation of the signaling pathway reduced PIκB expression levels and increased TNF-α and IL-6 expression levels in intestinal tissues. Our results demonstrated that a short-term high-fat diet induces activation of the TLR4/NF-κB signaling pathway in intestinal tissues, which causes local intestinal low-grade inflammation. These data improve our understanding of the molecular events involved in intestinal low-grade inflammation, which may be the triggering factor for chronic systemic low-grade inflammation.

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