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1.
Clin Neurol Neurosurg ; 197: 106078, 2020 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-32673897

RESUMEN

OBJECTIVES: Sporadic endolymphatic sac tumor (ELST) is rare. The purpose of this study was to analyze the clinical feature and surgical outcome of a single-center series of sporadic ELSTs. PATIENTS AND METHODS: We retrospectively reviewed all cases of sporadic ELSTs operated at the Tiantan hospital between 2010 and 2020. RESULTS: Retrospective record revealed 14 patients with sporadic ELSTs who underwent surgical management. Serum VEGF(vascular endothelial growth factor)values of 6 patients were measured on admission, and the levels were all higher than normal(0-160 ng/L, Enzyme-Linked ImmunoSorbent Assay). All patients underwent surgical treatments, and there was no significant difference in KPS before and after the operation(KPS = 88:88). The average maximum diameter of the tumor was 41 mm (range28-56 mm). After surgery, Two patients received radiation therapy, We did not encounter any case with metastatic dissemination. No deaths occurred during follow-up. CONCLUSION: ELST is defined as an aggressive or low-grade malignant tumor, with the characteristics of local recurrence and invasion of bone growth. Sporadic cases are common, and this rare tumor provides insight into the relationship between ELST and VHL syndrome. Early surgical resection can prevent or reduce the disability of auditory vestibular symptoms, and improve the chance of complete resection and retention of hearing. As far as possible complete resection of the tumor can prolong the disease-free survival of patients. Preoperative radiotherapy or stereotactic therapy cannot control tumor growth. Postoperative radiotherapy may have a positive effect on tumor control. The high expression of VEGF in patients may be related to tumor occurrence and development.

2.
Med Sci Monit ; 25: 3716-3727, 2019 May 19.
Artículo en Inglés | MEDLINE | ID: mdl-31104065

RESUMEN

BACKGROUND The aim of this study was to identify gene signals for lower-grade glioma (LGG) and to assess their potential as recurrence biomarkers. MATERIAL AND METHODS An LGG-related mRNA sequencing dataset was downloaded from The Cancer Genome Atlas (TCGA) Informix. Multiple bioinformatics analysis methods were used to identify key genes and potential molecular mechanisms in recurrence of LGG. RESULTS A total of 326 differentially-expressed genes (DEGs), were identified from 511 primary LGG tumor and 18 recurrent samples. Gene ontology (GO) analysis revealed that the DEGs were implicated in cell differentiation, neuron differentiation, negative regulation of neuron differentiation, and cell proliferation in the forebrain. The Kyoto Encyclopedia of Genes and Genomes (KEGG) database suggests that DEGs are associated with proteoglycans in cancer, the Wnt signaling pathway, ECM-receptor interaction, the PI3K-Akt signaling pathway, transcriptional deregulation in cancer, and the Hippo signaling pathway. The hub DEGs in the protein-protein interaction network are apolipoprotein A2 (APOA2), collagen type III alpha 1 chain (COL3A1), collagen type I alpha 1 chain (COL1A1), tyrosinase (TYR), collagen type I alpha 2 chain (COL1A2), neurotensin (NTS), collagen type V alpha 1 chain (COL5A1), poly(A) polymerase beta (PAPOLB), insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1), and anomalous homeobox (ANHX). GSEA revealed that the following biological processes may associated with LGG recurrence: cell cycle, DNA replication and repair, regulation of apoptosis, neuronal differentiation, and Wnt signaling pathway. CONCLUSIONS Our study demonstrated that hub DEGs may assist in the molecular understanding of LGG recurrence. These findings still need further molecular studies to identify the assignment of DEGs in LGG.


Asunto(s)
Neoplasias Encefálicas/genética , Neoplasias Encefálicas/metabolismo , Glioma/genética , Glioma/metabolismo , Biomarcadores , Neoplasias Encefálicas/patología , Biología Computacional/métodos , Bases de Datos Genéticas , Perfilación de la Expresión Génica/métodos , Ontología de Genes , Redes Reguladoras de Genes , Glioma/patología , Humanos , Clasificación del Tumor , Mapeo de Interacción de Proteínas/métodos , Mapas de Interacción de Proteínas , Transcriptoma
3.
FEBS Open Bio ; 9(4): 653-667, 2019 04.
Artículo en Inglés | MEDLINE | ID: mdl-30984540

RESUMEN

Glioma is one of the most common types of malignant primary central nervous system tumor, and prognosis for this disease is poor. As autophagic drugs have been reported to induce glioma cell death, we investigated the potential prognostic role of autophagy-associated long non-coding RNA (lncRNA) in glioma patients. In this study, we obtained 879 lncRNAs and 216 autophagy genes from the Chinese Glioma Genome Atlas microarray, and found that 402 lncRNAs are correlated with the autophagy genes. Subsequently, 10 autophagy-associated lncRNAs with prognostic value (PCBP1-AS1, TP53TG1, DHRS4-AS1, ZNF674-AS1, GABPB1-AS1, DDX11-AS1, SBF2-AS1, MIR4453HG, MAPKAPK5-AS1 and COX10-AS1) were identified in glioma patients using multivariate Cox regression analyses. A prognostic signature was then established based on these prognostic lncRNAs, dividing patients into low-risk and high-risk groups. The overall survival time was shorter in the high-risk group than that in the low-risk group [hazard ratio (HR) = 5.307, 95% CI: 4.195-8.305; P < 0.0001]. Gene set enrichment analysis revealed that the gene sets were significantly enriched in cancer-related pathways, including interleukin (IL) 6/Janus kinase/signal transducer and activator of transcription (STAT) 3 signaling, tumor necrosis factor α signaling via nuclear factor κB, IL2/STAT5 signaling, the p53 pathway and the KRAS signaling pathway. The Cancer Genome Atlas dataset was used to validate that high-risk patients have worse survival outcomes than low-risk patients (HR = 1.544, 95% CI: 1.110-2.231; P = 0.031). In summary, our signature of 10 autophagy-related lncRNAs has prognostic potential for glioma, and these autophagy-related lncRNAs may play a key role in glioma biology.


Asunto(s)
Autofagia/genética , Glioma/genética , ARN Largo no Codificante/análisis , China , Femenino , Regulación Neoplásica de la Expresión Génica , Glioma/diagnóstico , Humanos , Masculino , MicroARNs/genética , Pronóstico
4.
Mol Med Rep ; 17(3): 3658-3666, 2018 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-29257326

RESUMEN

Anoikis resistance is a crucial step in the process of tumor metastasis. This step determines whether the tumor cells will survive when they become detached from the extracellular matrix. However, the specific mechanism of tumor cells to bypass anoikis and become resistant remains to be elucidated. The present study aimed to determine the internal mechanism of bypassing anoikis through comparison of human osteosarcoma cell lines with human normal cell lines. High activating transcription factor 4 (ATF4) and myelocytomatosis oncogene (MYC) expression levels were observed in MG­63 and U­2 OS human osteosarcoma cell lines. It is possible that ATF4 and MYC contribute to tumor progression. Subsequently, the expression levels of ATF4 and MYC in HUVEC and CHON­001 human normal cell lines were upregulated and their adhesion abilities were reduced; whereas their ability to bypass anoikis increased significantly. Simultaneously, after we Following a knock­down ofATF4 and MYC expression levels in MG­63 and U­2 OS human osteosarcoma cell lines, their adhesion ability increased and their ability to bypassing anoikis was significantly reduced. Upregulation of MYC resulted in an upregulation of ATF4, and chromatin immunoprecipitation and luciferase reporter gene technology demonstrated that MYC binds to the promoter of ATF4. These findings suggest that ATF4 regulated by MYC might contribute to resistance to anoikis in human osteosarcoma cells.


Asunto(s)
Factor de Transcripción Activador 4/metabolismo , Anoicis , Proteínas Proto-Oncogénicas c-myc/metabolismo , Factor de Transcripción Activador 4/antagonistas & inhibidores , Factor de Transcripción Activador 4/genética , Neoplasias Óseas/metabolismo , Neoplasias Óseas/patología , Adhesión Celular , Línea Celular Tumoral , Células Endoteliales de la Vena Umbilical Humana , Humanos , Osteosarcoma/metabolismo , Osteosarcoma/patología , Regiones Promotoras Genéticas , Unión Proteica , Interferencia de ARN , ARN Interferente Pequeño/metabolismo , Regulación hacia Arriba
5.
Exp Ther Med ; 13(6): 3417-3423, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-28587420

RESUMEN

MicroRNAs (miRs), a class of small non-coding RNAs, function as key regulators in gene expression through binding to the 3'-untranslated region (UTR) of their target mRNA, which further leads to translational repression or mRNA degradation. Recently, miR-138 has been found to have a tumor suppressive role in a variety of human malignancies. However, the exact role of miR-138 in regulating the malignant phenotypes of osteosarcoma (OS) has remained to be elucidated. In the present study, reverse-transcription PCR analysis showed that the expression of miR-138 was markedly reduced in OS tissues compared to that in matched adjacent non-tumorous tissues. Furthermore, it was also downregulated in several common OS cell lines, when compared with that in a normal human osteoblast cell line. Overexpression of miR-138 suppressed cell proliferation and invasion and led to a significant decrease in the protein expression of sirtuin 1 (SIRT1), which was further identified as a direct target gene of miR-138 in MG63 cells. Moreover, restoration of SIRT1 expression reversed the suppressive effects of miR-138 on MG63 cell proliferation and invasion. Finally, the expression of SIRT1 was found to be significantly upregulated in OS tissues compared to that in matched adjacent tissues, and SIRT1 levels were inversely correlated with the miR-138 levels in OS tissues. Therefore, the present study demonstrated that miR-138 has a role in inhibiting OS cell proliferation and invasion via directly targeting SIRT1, and suggested that the miR-138/SIRT1 axis may become a promising therapeutic target for OS.

6.
J Craniofac Surg ; 28(4): 985-987, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-28328602

RESUMEN

OBJECTIVES: To help surgeons locating anatomic landmarks when performing endoscopic endonasal transcribriform approach to anterior skull base. METHODS: High-resolution axial computed tomography (CT) images at thickness of 0.6 mm, and reconstructed 0.41-mm thick gapless sagittal and coronal CT images were taken from 123 subjects. Using mimics software, first located elementary points and line: nasal spine, midpoint of posterior hard palate and the line between them; then located measured points right/left posterior and anterior points; measured distances between measured points and from measured points to nasal spine and angles between lines connecting measured points to nasal spine and the basic line. RESULTS: The distances from nasal spine to right/left posterior and anterior points of anterior skull base are 68.67 ± 6.04 and 61.71 ± 5.09 mm, corresponding angles are 45.89 ± 4.20° and 72.07 ± 4.06°, respectively. The width and length of defect of anterior skull base are 24.45 ± 2.62 and 31.03 ± 4.96 mm; its area ranges from 373.75 ± 94.08 to 800.91 ± 195.07 mm. CONCLUSIONS: The study provides information about anterior skull base anatomic landmarks, which can help surgeons to locate them and avoid relative complications during endoscopic endonasal transcribriform approach to anterior skull base. The measurements can be used as surgical indicators to investigate the landmarks.


Asunto(s)
Puntos Anatómicos de Referencia/diagnóstico por imagen , Nariz/anatomía & histología , Base del Cráneo/anatomía & histología , Adolescente , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Cirugía Endoscópica por Orificios Naturales , Nariz/diagnóstico por imagen , Paladar Duro/anatomía & histología , Paladar Duro/diagnóstico por imagen , Base del Cráneo/diagnóstico por imagen , Base del Cráneo/cirugía , Tomografía Computarizada por Rayos X , Adulto Joven
7.
Exp Ther Med ; 12(5): 2815-2822, 2016 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-27882079

RESUMEN

Caveolin-1 (CAV-1), which is an oncoprotein and a tumor suppressor, is highly expressed in normal osteoblasts. Although researchers have investigated its role in human osteosarcoma, the mechanism of caveolin-1 action in osteosarcoma remains unknown. In the present study, Saos-2 and U-2 OS cells were cultured with a continuous induction protocol of gradually increasing Taxol concentration for 6 months to establish drug-resistant cell lines. CAV-1 expression levels in osteosarcoma cells were detected via western blotting and quantitative polymerase chain reaction. CAV-1 knockdown was achieved using a short hair-pin RNA lentivirus vector, and cell viability was analyzed by MTT assay. The effect of caveolin-1 on autophagy was investigated, and the downregulation of caveolin-1 and increased autophagy was identified in Taxol-resistant osteosarcoma cells. In addition, the results of the present study demonstrated that downregulation of caveolin-1 promotes autophagy and induces osteosarcoma cell resistance to Taxol. Notably, overexpression of CAV-1 resensitized drug-resistant cells to Taxol via declined autophagy. In conclusion, CAV-1 was demonstrated to be downregulated in Taxol-resistant osteosarcoma cells, and overexpression of CAV-1 in human osteosarcoma cells suppressed Taxol resistance by attenuating PI3K-Akt-JNK-dependent autophagy. The present findings suggest that further investigation into CAV-1's role in Taxol resistance is warranted. In the future, detection of CAV-1 may be used as an indicator to evaluate the treatment and prognosis of patients with osteosarcoma.

8.
Biochem Biophys Res Commun ; 478(1): 74-80, 2016 09 09.
Artículo en Inglés | MEDLINE | ID: mdl-27453337

RESUMEN

Macroautophagy (herein termed autophagy) works as a protective mechanism in tumorigenesis and development under metabolic stress condition. Multitudes of genes have been found involved in this process during past decades. In the present study, we report that Wilm's tumor suppressor1 (WT1) is involved in autophagy in osteosarcoma (OS) cells. WT1, a transcription factor with multitude of target genes, expresses in a majority of cancer types. Though wide-ranging effect of WT1 is now well documented, the function of WT1 in tumors remains poorly defined. In this chapter, it is found that high expression of WT1 positively correlates with active autophagy in human osteosarcoma cells. And further study on cell signaling pathway illustrates that Akt/JNK pathway acts as a positive regulator of autophagy induced by WT1. Here, we present evidence that WT1 modulates Akt/JNK signaling pathway mediated autophagy by controlling the expression of growth arrest-specific 1 (Gas1). We show that WT1 is required for Gas1 transcription in osteosarcoma cells. And Gas1 is upregulated followed WT1 overexpression in a time-dependent manner. Loss of Gas1 results in a reduction of WT1-induced autophagy.


Asunto(s)
Autofagia , Neoplasias Óseas/metabolismo , Proteínas de Ciclo Celular/genética , Sistema de Señalización de MAP Quinasas , Osteosarcoma/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Proteínas WT1/metabolismo , Neoplasias Óseas/genética , Neoplasias Óseas/patología , Huesos/metabolismo , Huesos/patología , Proteínas de Ciclo Celular/metabolismo , Línea Celular Tumoral , Proteínas Ligadas a GPI/genética , Proteínas Ligadas a GPI/metabolismo , Regulación Neoplásica de la Expresión Génica , Humanos , Osteosarcoma/genética , Osteosarcoma/patología , Transducción de Señal , Proteínas WT1/genética
9.
Eur Neurol ; 70(1-2): 124-32, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23859844

RESUMEN

The efficacy and safety of intra-arterial (IA) chemotherapy versus intravenous (IV) chemotherapy for malignant gliomas were studied. We searched eight electronic databases to identify relevant randomized controlled trials that compared IA chemotherapy with IV chemotherapy in patients with malignant gliomas. This study was conducted in compliance with the Quality of Reporting of Meta-analysis (QUORUM) guidelines. The quality of data was assessed using the Grades of Recommendation, Assessment, Development, and Evaluation (GRADE) approach. Four eligible randomized controlled trials including 460 patients were retrieved. Comparing IA chemotherapy and IV chemotherapy for malignant gliomas, disease control rate, efficacy rate, 1-year, 2-year, and 3-year overall survival as well as grade 3/4 leukocytopenia, thrombocytopenia, and anemia were not statistically different. In conclusion, IA chemotherapy is not superior to IV chemotherapy in terms of efficacy and overall survival as a treatment for malignant gliomas.


Asunto(s)
Antineoplásicos/administración & dosificación , Neoplasias Encefálicas/tratamiento farmacológico , Glioma/tratamiento farmacológico , Ensayos Clínicos Controlados Aleatorios como Asunto , Neoplasias Encefálicas/mortalidad , Glioma/mortalidad , Humanos , Infusiones Intraarteriales , Infusiones Intravenosas , Resultado del Tratamiento
10.
Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi ; 24(12): 554-5, 558, 2010 Jun.
Artículo en Chino | MEDLINE | ID: mdl-20806861

RESUMEN

OBJECTIVE: To explore the relationship between extend types and distant metastasis of nasopharyngeal carcinoma (NPC). METHOD: Retrospective analyze 260 patients with nasopharyngeal carcinoma, among which 162 cases were distant metastasis (metastasis group) and 98 cases were neither distant metastasis nor recurrence (disease-free group) over 5 years after radiotherapy. All these patients were staged depending on CT or MRI image before treatment and divided into local-regional type(T(1-2)N(0-1)) for 36 cases and upward invasion type (T(3-4)N(0-1)) for 68 cases and downward invasion type (T(1-2)N(2-3)) for 75 cases and mixed type (T(3-4)N(2-3)) for 81 cases. The differences between two groups was analyzed using Chi-square test. RESULT: The local-regional type and upward invasion type was 25.3% for the metastasis group and 64.3% for the disease-free group. The downward and mixed invasion was 74.7% for the metastasis group and 35.7% for the disease-free group. The rate(proportion) of N(2-3) was significantly higher in metastasis group than in disease-free group with limited extension (84.4% vs. 33.3%, P < 0.01). The rates(proportion) of N(0-1) and N(2-3) were also significantly higher in metastasis group than in disease-free group with severity extension (T(3-4) (60% vs. 36.1% and 68.4% vs. 40%, P < 0.01). CONCLUSION: The extent of cervical lymph node metastases is one of the most important factors of NPC with distant metastasis, severity extension of primary disease should also be in consideration. Even the limitations of primary disease, once cervical lymph node metastasis occurs, the risk of distant metastasis is significantly increased.


Asunto(s)
Metástasis Linfática , Neoplasias Nasofaríngeas , Adolescente , Adulto , Anciano , Carcinoma , Femenino , Humanos , Ganglios Linfáticos/patología , Masculino , Persona de Mediana Edad , Carcinoma Nasofaríngeo , Neoplasias Nasofaríngeas/patología , Cuello , Metástasis de la Neoplasia , Estadificación de Neoplasias , Pronóstico , Estudios Retrospectivos , Adulto Joven
11.
Artículo en Chino | MEDLINE | ID: mdl-19670613

RESUMEN

OBJECTIVE: To summarize the experience of cervical trachea sleeve resection. METHOD: Twelve cases of sleeve resection between January 1985 and December 2006 were retrospectively analyzed. Six cases were adenoid cystic carcinoma, four cases were squamous cell carcinoma, one case was adenocarcinoma and one case was cervical tracheomalacia. Length of cervical trachea resection is three to six cm with primary anastomosis, among which five cases were over five cm, seven cases underwent laryngeal release and three cases underwent subtotal thyroidectomy. Nine cases were tracheal anastomotic stoma enwrapped with normal thyroid tissue and three cases were not. RESULT: Laryngeal function and normal phonation were saved, anastomotic stoma healed up, and the tracheal mucosa appear normal in all the patients . The three years survival rate and five years' survival rate were 85.37% and 56.61% for the patients respectively. CONCLUSION: Cervical trachea sleeve resection accorded with the principle of tracheal tumors therapy. It was conducive to healing of the tracheal anastomosis.


Asunto(s)
Carcinoma Adenoide Quístico/cirugía , Tráquea/cirugía , Neoplasias de la Tráquea/cirugía , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos
12.
Artículo en Chino | MEDLINE | ID: mdl-18330260

RESUMEN

OBJECTIVE: To investigate the effects of situation of misdiagnosis of nasopharyngeal carcinoma (NPC) on distant metastasis. METHOD: The history of diagnosis and treatment of 85 newly diagnosed cases with nasopharyngeal carcinoma were studied by using itemized questionnaire purposely; 433 patients with different prognosis were analyzed retrospectively for the misdiagnoses and mistreatment, including surgical biopsy in the neck. RESULT: (1) The rate of misdiagnosis of 85 patients was 72.64%, and the percentage decreased as the level of the hospitals increased; the majority of the patients (77.36%) were diagnoses within 1 month after the first symptom had appeared; the number of diseases misdiagnosed was 20, most common of which were lymphnoditis, tuberculosis of lymph node and secretory tympanitis; (2) Our data showed that among 433 patients analysed retrospectively, 60 cases had undergone surgical biopsy in the neck, 75% of whom had never received nasopharyngeal biopsy; 43 cases had underwent nasopharyngeal biopsy after the pathological diagnosis as metastatic carcinoma of neck biopsy (71.67%) and the rest (20.0%) received radiotherapy directly or after negative nasopharyngeal biopsy for merely 1 to 4 times; of those 43 cases who were diagnosed as NPC by nasopharyngeal biopsy, 79.17% got positive results at first sampling. (3) Rate of misdiagnosis and mistreatment including surgical biopsy in the neck of patients who had been tumor-free for 5 years or above was significantly lower than that of those who experienced distant metastasis after or before treatment (P < 0.05). CONCLUSIONS: Misdiagnosis and mistreatment including biopsy by surgery of neck is common even in high-grade hospitals; it is doctor that is responsible for this situation; the high occurrence rate of misdiagnosis and mistreatment, biopsy by neck surgery, especially the delayed treatment after the neck biopsy are the factors that contribute to distant metastasis of NPC.


Asunto(s)
Errores Diagnósticos , Neoplasias Nasofaríngeas/diagnóstico , Neoplasias Nasofaríngeas/patología , Humanos , Neoplasias Nasofaríngeas/terapia , Metástasis de la Neoplasia , Estadificación de Neoplasias , Pronóstico , Estudios Retrospectivos , Resultado del Tratamiento
13.
Lin Chuang Er Bi Yan Hou Ke Za Zhi ; 20(11): 494-5, 2006 Jun.
Artículo en Chino | MEDLINE | ID: mdl-16929828

RESUMEN

OBJECTIVE: To explore the relationship between the expression of estrogen and progestogen receptors (ER/PR) in distant metastasis of nasopharyngeal carcinoma (NPC). METHOD: The expression of ER/PR were detected with immunohistochemical SP method in 81 nasopharyngeal carcinoma who had been treated and followed-up. RESULT: (1) The positive rates of ER and PR in the tissue of NPC were 35.8% and 53.1%. (2) The expression intensity of PR in T stage has obvious statistically significance (P < 0.01), the expression intensity and positive rates of ER in IVb stage were obviously higher than the other stage (P < 0.01). (3) The positive rates of ER and PR in patients of the distant metastasis group (including the distant metastasis found before and after radiotherapy) were obviously higher than the group who have been alive with no evidence of disease for five or more than five years after radiotherapy (P < 0.01) and P < 0.05). CONCLUSION: The positive expression of ER and PR in NPC were well correlated with the distant metastasis, and it increased gradually. Positive expression, especially the strong positive expression points out the bad prognosis. Endocrine treat may reduce and postpone the distant metastasis.


Asunto(s)
Neoplasias Nasofaríngeas/metabolismo , Neoplasias Nasofaríngeas/patología , Receptores Estrogénicos/metabolismo , Receptores de Progesterona/metabolismo , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Persona de Mediana Edad , Metástasis de la Neoplasia , Estadificación de Neoplasias , Adulto Joven
14.
Lin Chuang Er Bi Yan Hou Ke Za Zhi ; 20(3): 113-5, 2006 Feb.
Artículo en Chino | MEDLINE | ID: mdl-16646402

RESUMEN

OBJECTIVE: To study the relation between Mta1 gene (metastasis associated gene, meta gene) and lymph node metastasis of laryngeal carcinomas. METHOD: Reverse transcription polymerase chain reaction(RT-PCR) technique was used to detect the expression of Mta1 mRNA in 30 carcinoma of larynx tissue cases, 12 lymph nodes and 10 normal larynx tissue cases. RESULT: The frequency of MTA1 overexpression was 100% (8/8) in primary carcinomas of larynx with metastasis but only 22.7% (5/22) in toes without metastasis (P<0.01). Expression of MTA1 was 87.5%(7/8) in lymph nodes with metastasis but only 0(0/4) in toes without metastasis(P<0.05). The frequency of Mta1 overexpression was 43.3% (13/30) in primary carcinomas of larynx. CONCLUSION: Our data suggest that the overexpression of the MTA1 gene correlates with lymph node metastasis of carcinomas of larynx. Mta1 gene will probably become a valuable gene for judging metastasis of laryngeal carcinoma.


Asunto(s)
Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patología , Histona Desacetilasas/genética , Neoplasias Laríngeas/genética , Neoplasias Laríngeas/patología , Proteínas Represoras/genética , Adulto , Anciano , Expresión Génica , Regulación Neoplásica de la Expresión Génica , Humanos , Metástasis Linfática , Masculino , Persona de Mediana Edad , ARN Mensajero/genética , Transactivadores
15.
Lin Chuang Er Bi Yan Hou Ke Za Zhi ; 20(4): 164-5, 2006 Feb.
Artículo en Chino | MEDLINE | ID: mdl-16711441

RESUMEN

OBJECTIVE: To explore the duration related to distant metastases of nasopharyngeal carcinoma (NPC). METHOD: One hundred and three cases with confirmative diagnosis of NPC, in which the distant metastasis had happened before radiotherapy, were called the natural metastasis group. Another one hundred and thirty-eight cases, in which the distant metastasis had happened after radiotherapy, were called the metastasis after radiotherapy group. The clinical data and appearance time on distant metastasis from the two groups were analyzed. RESULT: It took 10.78 months (95% CI: 8.68-12.88) on average in the natural metastasis group from the primary symptom to the distant metastasis, and 20.77 months on average in the metastasis after radiotherapy group. Comparing the two groups, there was highly remarkable difference (P < 0.01). The distant metastasis happened in 91.26% of the cases in the natural metastasis group in 2 years. And in 1 year and in 2 years, the distal metastasis incidences of the natural metastasis group was higher than those of the metastasis after radiotherapy group by 34.41% and 28.94%, respectively. The distant metastasis happened in all cases in the natural metastasis group in 5 years, while in 2.9% of the cases in the metastasis after radiotherapy group beyond 5 years. There wasn't any obvious difference in the patients' sex and the appearance rate of the primary symptom (P > 0.05). But in the metastasis after radiotherapy group, the patients were younger and the T and N stages were earlier. CONCLUSION: The distal metastasis in the metastasis after radiotherapy group was at least 1-2 years later than that in the natural metastasis group. It was perhaps relative to the younger patients and the earlier stages.


Asunto(s)
Neoplasias Nasofaríngeas/patología , Neoplasias Nasofaríngeas/radioterapia , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Persona de Mediana Edad , Metástasis de la Neoplasia , Adulto Joven
16.
Lin Chuang Er Bi Yan Hou Ke Za Zhi ; 19(17): 785-7, 2005 Sep.
Artículo en Chino | MEDLINE | ID: mdl-16334253

RESUMEN

OBJECTIVE: To study the relation between T lymphoma invasion/metastasis1 (Tiam1) gene and nasopharyngeal carcinoma progression. METHOD: Using of reverse transcription polymerase chain (RT-PCR) technique on 41 nasopharyngeal carcinoma tissue and 20 normal nasopharyngeal tissue to proceed the test of mRNA overexpression. RESULT: Average expression of the Tiam1 gene in nasopharyngeal carcinoma tissue (1.183 +/- 0.73) is higher than in normal nasopharyngeal tissue (0.87 +/- 0.45) (P < 0.01). The positive relationship between the frequency of Tiam1 overexpression and clinical staging and T staging was very significant (P < 0.05) and increased gradually; The frequency of Tiam1 overexpression was 59.3% (16/27) in patients with lymph node metastasis, 21.4% (3/ 14) in patients without lymph node metastasis, overexpression between them was very significant (P < 0.05). CONCLUSION: Average expression of the Tiam1 gene in nasopharyngeal carcinoma tissue is higher than in normal nasopharyngeal tissue. Our data suggest that the overexpression of the Tiam1 gene correlates invasion and metastasis of nasopharyngeal carcinoma.


Asunto(s)
Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patología , Factores de Intercambio de Guanina Nucleótido/metabolismo , Neoplasias Nasofaríngeas/genética , Neoplasias Nasofaríngeas/patología , Adulto , Anciano , Carcinoma de Células Escamosas/metabolismo , Estudios de Casos y Controles , Femenino , Expresión Génica , Factores de Intercambio de Guanina Nucleótido/genética , Humanos , Metástasis Linfática , Masculino , Persona de Mediana Edad , Neoplasias Nasofaríngeas/metabolismo , Invasividad Neoplásica , Estadificación de Neoplasias , ARN Mensajero/genética , Proteína 1 de Invasión e Inducción de Metástasis del Linfoma-T , Adulto Joven
17.
Zhonghua Zhong Liu Za Zhi ; 27(8): 505-6, 2005 Aug.
Artículo en Chino | MEDLINE | ID: mdl-16188155

RESUMEN

OBJECTIVE: To investigate the prevalent area where the nasopharyngeal carcinoma (NPC) initially would develop most frequently in the nasopharynx. METHODS: From March, 1994 to June, 2003, the data of original micro-focus of 32 pathologically confirmed NPC were retrospectively reviewed, All cases were shown by CT to have stages T(0) or T(1) lesions. RESULTS: On clinical examination, only 3 cases (9.4%) were found to have original micro-tumor in the recess, the other 29 cases (90.6%) had developed from the other regions including 20 (69.0%) from the roof and 9 cases (31.0%) from the posterior wall, all with the mucosa in the recess smooth and symmetrical. However, CT images showed that membrane of all the nasopharyngeal walls, including the recess, were normal in 24 cases (75.0%); except the micro-foci were observed on one side of the posterior wall in 5 cases (15.6%) but still with the recess normal, One lateral wall and/or the recess were involved with abnormal appearance in 3 cases (9.4%) who were clinically found to have NPC focus originated from the recess. CONCLUSION: Our data suggest that the roof of nasopharyngeal cavity may be the area likely to develop the original NPC micro-focus, followed in frequency by the posterior wall, with the recess the least likely. The recess involvement observed in advanced lesions may be the extension of the NPC focus from the original site on the roof or the posterior wall of nasopharynx.


Asunto(s)
Neoplasias Nasofaríngeas/diagnóstico por imagen , Nasofaringe/diagnóstico por imagen , Nasofaringe/patología , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Neoplasias Nasofaríngeas/patología , Estudios Retrospectivos , Tomografía Computarizada por Rayos X
18.
Lin Chuang Er Bi Yan Hou Ke Za Zhi ; 19(20): 926-8, 2005 Oct.
Artículo en Chino | MEDLINE | ID: mdl-16398047

RESUMEN

OBJECTIVE: The aim of this study was to study the correlation between local and regional infiltration and distant metastasis of nasopharyngeal carcinoma(NPC). METHOD: Materials of 204 NPC cases primarily treated (including 101 cases with tumor free and a period of survived time for 5 or more years after radiotherapy and 103 cases with distant metastasis after radiotherapy ). The extent and the way of local aggressiveness were made sure by studying their CT. Then the single-factor and COX analysis were adopted to analyze. RESULT: Single-factor analysis showed the extent of aggressiveness of nasal sinuses, base of skull, cranial nerves, parapharyngeal space, laterals and number, size, fixation of neck lymph nodes of metastasis, clinical stage, T and N stage had statistical significance (P <0.05); Multi-factor analysis merely showed aggressiveness of nasal sinuses, cranial nerves, laterals and number, size of neck lymph nodes, clinical stage had statistical significance; Analysis of correlation between the situations of local aggressiveness and regional infiltration showed that the occurrence of metastasis of lymph nodes and its degree in metastasis group of post-radiotherapy was obviously higher than that in non-metastasis group (P < 0.05, P < 0.01), while the degree of local aggressiveness, the involvement of base of skull and the parapharyngeal space were not. CONCLUSION: Regional infiltration and its degree are the most important and stable factors that affect the distant metastasis in NPC patients after radiotherapy, and primary lesion intrigues distant metastasis mainly through occurrence of metastasis of regional lymph nodes.


Asunto(s)
Neoplasias Nasofaríngeas/patología , Adulto , Supervivencia sin Enfermedad , Femenino , Estudios de Seguimiento , Humanos , Masculino , Persona de Mediana Edad , Invasividad Neoplásica , Metástasis de la Neoplasia , Estadificación de Neoplasias , Pronóstico , Modelos de Riesgos Proporcionales
19.
Zhonghua Zhong Liu Za Zhi ; 25(5): 501-3, 2003 Sep.
Artículo en Chino | MEDLINE | ID: mdl-14575581

RESUMEN

OBJECTIVE: To investigate the management and prognosis of thyroid well-differentiated carcinoma invading the upper aerodigestive tract. METHODS: A retrospective analysis of the management was performed done 62 patients with thyroid well-differentiated carcinoma invading the upper aerodigestive tract. The main method of surgery was shaving excision, and the other means including partial thyrochondrectomy, total laryngectomy, sleeve tracheal resection, sternocleidomastoid myoperiosteal flap and myodermal flap reconstruction, or simply palliative excision. Some patients received postoperative radioactive isotope therapy and radiotherapy. All patients were followed-up for 2 to 15 years with an average of 6.5 years. RESULTS: The best curative effect was proved in the patients with local invasion, with the lumen uninvolved and their locoregional control rate was 100.0% (17/17). And the second choice was in patients with more extensive involvement of the upper aerodigestive tract structures. For them, extensive surgical management was done attempting to remove all gross disease followed by reconstruction, their locoregional control rate was 87.5% (7/8). And the third place was designated to patients with local invasion for which shaving excision was performed even though minor residual disease was left, their locoregional control rate was 55.6% (5/9). The poorest result went to simple palliative excision. For 17 patients with minor residual tumor, the locoregional control rate of those who were given postoperative radioactive isotope therapy was significantly higher than those without. CONCLUSION: According to the limits and degree of invasion in the upper aerodigestive tract by thyroid well-differentiated carcinoma, different ways of surgery is indicated. For patients with residual disease, radioactive isotope therapy should be used to improve the result and life quality. Advanced lesions should be given postoperative radio therapy.


Asunto(s)
Sistema Digestivo/patología , Sistema Respiratorio/patología , Neoplasias de la Tiroides/terapia , Adolescente , Adulto , Anciano , Terapia Combinada , Femenino , Humanos , Masculino , Persona de Mediana Edad , Invasividad Neoplásica , Estudios Retrospectivos , Tasa de Supervivencia , Neoplasias de la Tiroides/mortalidad , Neoplasias de la Tiroides/patología
20.
Lin Chuang Er Bi Yan Hou Ke Za Zhi ; 16(10): 537-8, 2002 Oct.
Artículo en Chino | MEDLINE | ID: mdl-15515564

RESUMEN

OBJECTIVE: To explore the clinical feature of benign symmetric lipomatosis (BSL) so as to improve its diagnosis and treatment. METHOD: 28 patients of BSL treated in our hospital were analyzed and literature was reviewed. Surgical management is used in all patients. RESULT: There were no patients died. All patients were satisfactory with their appearance and function improved. 28 patients were followed up for 2-15 years (average 6 years and 5 months), only 5 cases recurred. CONCLUSION: BSL usually occurs in middle age men. Patients have a striking appearance "hump back" because of a diffuse, symmetric distribution of the lipomatous tissue in the cervical region. The etiology is related to alcohol abuse. Operation is the most effective treatment. The radical surgical therapy should not be emphasized because the important anatomic structures may be damaged. Abstaining from alcohol may help to reduce the rate of recurrence.


Asunto(s)
Lipomatosis Simétrica Múltiple/cirugía , Adulto , Anciano , Femenino , Estudios de Seguimiento , Humanos , Lipomatosis Simétrica Múltiple/diagnóstico , Lipomatosis Simétrica Múltiple/etiología , Masculino , Persona de Mediana Edad
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