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1.
Proc Natl Acad Sci U S A ; 118(11)2021 03 16.
Artículo en Inglés | MEDLINE | ID: mdl-33707213

RESUMEN

Marine picocyanobacteria of the genus Prochlorococcus are the most abundant photosynthetic organisms in the modern ocean, where they exert a profound influence on elemental cycling and energy flow. The use of transmembrane chlorophyll complexes instead of phycobilisomes as light-harvesting antennae is considered a defining attribute of Prochlorococcus Its ecology and evolution are understood in terms of light, temperature, and nutrients. Here, we report single-cell genomic information on previously uncharacterized phylogenetic lineages of this genus from nutrient-rich anoxic waters of the eastern tropical North and South Pacific Ocean. The most basal lineages exhibit optical and genotypic properties of phycobilisome-containing cyanobacteria, indicating that the characteristic light-harvesting antenna of the group is not an ancestral attribute. Additionally, we found that all the indigenous lineages analyzed encode genes for pigment biosynthesis under oxygen-limited conditions, a trait shared with other freshwater and coastal marine cyanobacteria. Our findings thus suggest that Prochlorococcus diverged from other cyanobacteria under low-oxygen conditions before transitioning from phycobilisomes to transmembrane chlorophyll complexes and may have contributed to the oxidation of the ancient ocean.


Asunto(s)
Complejos de Proteína Captadores de Luz/genética , Oxígeno/análisis , Prochlorococcus/genética , Agua de Mar/microbiología , Clorofila/genética , Cianobacterias/clasificación , Cianobacterias/genética , Evolución Molecular , Genes Bacterianos/genética , Genoma Bacteriano/genética , Nutrientes/análisis , Océano Pacífico , Ficobilisomas/genética , Filogenia , Pigmentos Biológicos/genética , Prochlorococcus/clasificación , Agua de Mar/química
3.
Nat Commun ; 10(1): 5742, 2019 12 17.
Artículo en Inglés | MEDLINE | ID: mdl-31848334

RESUMEN

Small animals support a wide range of pathological phenotypes and genotypes as versatile, affordable models for pathogenesis of cardiovascular diseases and for exploration of strategies in electrotherapy, gene therapy, and optogenetics. Pacing tools in such contexts are currently limited to tethered embodiments that constrain animal behaviors and experimental designs. Here, we introduce a highly miniaturized wireless energy-harvesting and digital communication electronics for thin, miniaturized pacing platforms weighing 110 mg with capabilities for subdermal implantation and tolerance to over 200,000 multiaxial cycles of strain without degradation in electrical or optical performance. Multimodal and multisite pacing in ex vivo and in vivo studies over many days demonstrate chronic stability and excellent biocompatibility. Optogenetic stimulation of cardiac cycles with in-animal control and induction of heart failure through chronic pacing serve as examples of modes of operation relevant to fundamental and applied cardiovascular research and biomedical technology.


Asunto(s)
Ingeniería Biomédica/métodos , Dispositivos de Terapia de Resincronización Cardíaca , Insuficiencia Cardíaca/etiología , Miniaturización , Optogenética/métodos , Animales , Modelos Animales de Enfermedad , Suministros de Energía Eléctrica , Femenino , Humanos , Preparación de Corazón Aislado , Masculino , Ratones , Ratones Transgénicos , Tecnología Inalámbrica
4.
Elife ; 62017 10 20.
Artículo en Inglés | MEDLINE | ID: mdl-29052541

RESUMEN

The temporal regulation of protein abundance and post-translational modifications is a key feature of cell division. Recently, we analysed gene expression and protein abundance changes during interphase under minimally perturbed conditions (Ly et al., 2014, 2015). Here, we show that by using specific intracellular immunolabelling protocols, FACS separation of interphase and mitotic cells, including mitotic subphases, can be combined with proteomic analysis by mass spectrometry. Using this PRIMMUS (PRoteomic analysis of Intracellular iMMUnolabelled cell Subsets) approach, we now compare protein abundance and phosphorylation changes in interphase and mitotic fractions from asynchronously growing human cells. We identify a set of 115 phosphorylation sites increased during G2, termed 'early risers'. This set includes phosphorylation of S738 on TPX2, which we show is important for TPX2 function and mitotic progression. Further, we use PRIMMUS to provide the first a proteome-wide analysis of protein abundance remodeling between prophase, prometaphase and anaphase.


Asunto(s)
Ciclo Celular , Proteoma/análisis , Proteómica/métodos , Línea Celular , Citometría de Flujo , Humanos , Inmunohistoquímica , Espectrometría de Masas
5.
Cell Syst ; 5(1): 38-52.e4, 2017 07 26.
Artículo en Inglés | MEDLINE | ID: mdl-28734827

RESUMEN

Starvation causes comprehensive metabolic changes, which are still not fully understood. Here, we used quantitative proteomics and RNA sequencing to examine the temporal starvation responses in wild-type Caenorhabditis elegans and animals lacking the transcription factor HLH-30. Our findings show that starvation alters the abundance of hundreds of proteins and mRNAs in a temporal manner, many of which are involved in central metabolic pathways, including lipoprotein metabolism. We demonstrate that premature death of hlh-30 animals under starvation can be prevented by knockdown of either vit-1 or vit-5, encoding two different lipoproteins. We further show that the size and number of intestinal lipid droplets under starvation are altered in hlh-30 animals, which can be rescued by knockdown of vit-1. Taken together, this indicates that survival of hlh-30 animals under starvation is closely linked to regulation of intestinal lipid stores. We provide the most detailed poly-omic analysis of starvation responses to date, which serves as a resource for further mechanistic studies of starvation.


Asunto(s)
Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/genética , Proteínas de Caenorhabditis elegans/genética , Caenorhabditis elegans/fisiología , Lipoproteínas/metabolismo , Inanición/metabolismo , Animales , Técnicas de Inactivación de Genes , Gotas Lipídicas , Tamaño de la Partícula , Proteómica , Interferencia de ARN , Análisis de Secuencia de ARN , Vitelogeninas/genética
6.
Sci Rep ; 7(1): 4314, 2017 06 28.
Artículo en Inglés | MEDLINE | ID: mdl-28659600

RESUMEN

Asymmetric cell divisions are required for cellular diversity and defects can lead to altered daughter cell fates and numbers. In a genetic screen for C. elegans mutants with defects in dopaminergic head neuron specification or differentiation, we isolated a new allele of the transcription factor HAM-1 [HSN (Hermaphrodite-Specific Neurons) Abnormal Migration]. Loss of both HAM-1 and its target, the kinase PIG-1 [PAR-1(I)-like Gene], leads to abnormal dopaminergic head neuron numbers. We identified discrete genetic relationships between ham-1, pig-1 and apoptosis pathway genes in dopaminergic head neurons. We used an unbiased, quantitative mass spectrometry-based proteomics approach to characterise direct and indirect protein targets and pathways that mediate the effects of PIG-1 kinase loss in C. elegans embryos. Proteins showing changes in either abundance, or phosphorylation levels, between wild-type and pig-1 mutant embryos are predominantly connected with processes including cell cycle, asymmetric cell division, apoptosis and actomyosin-regulation. Several of these proteins play important roles in C. elegans development. Our data provide an in-depth characterisation of the C. elegans wild-type embryo proteome and phosphoproteome and can be explored via the Encyclopedia of Proteome Dynamics (EPD) - an open access, searchable online database.


Asunto(s)
Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Neuronas Dopaminérgicas/metabolismo , Genómica , Fosfoproteínas/genética , Fosfoproteínas/metabolismo , Proteómica , Animales , Caenorhabditis elegans/embriología , Proteínas de Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/metabolismo , Cromatografía Liquida , Neuronas Dopaminérgicas/citología , Epistasis Genética , Regulación del Desarrollo de la Expresión Génica , Genómica/métodos , Espectrometría de Masas , Mutación , Proteínas del Tejido Nervioso/genética , Proteínas del Tejido Nervioso/metabolismo , Proteínas Serina-Treonina Quinasas/genética , Proteínas Serina-Treonina Quinasas/metabolismo , Proteómica/métodos , Transducción de Señal
7.
Cell Syst ; 3(2): 144-159, 2016 08.
Artículo en Inglés | MEDLINE | ID: mdl-27453442

RESUMEN

Effective network analysis of protein data requires high-quality proteomic datasets. Here, we report a near doubling in coverage of the C. elegans adult proteome, identifying >11,000 proteins in total with ∼9,400 proteins reproducibly detected in three biological replicates. Using quantitative mass spectrometry, we identify proteins whose abundances vary with age, revealing a concerted downregulation of proteins involved in specific metabolic pathways and upregulation of cellular stress responses with advancing age. Among these are ∼30 peroxisomal proteins, including the PRX-5/PEX5 import protein. Functional experiments confirm that protein import into the peroxisome is compromised in vivo in old animals. We also studied the behavior of the set of age-variant proteins in chronologically age-matched, long-lived daf-2 insulin/IGF-1-pathway mutants. Unexpectedly, the levels of many of these age-variant proteins did not scale with extended lifespan. This indicates that, despite their youthful appearance and extended lifespans, not all aspects of aging are reset in these long-lived mutants.


Asunto(s)
Proteoma , Animales , Caenorhabditis elegans , Proteínas de Caenorhabditis elegans , Proteómica , Transducción de Señal
8.
Mol Cell Proteomics ; 15(7): 2476-90, 2016 07.
Artículo en Inglés | MEDLINE | ID: mdl-27114452

RESUMEN

We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility during chromatographic separation. We show this efficiently detects both integral membrane and membrane-associated protein complexes,in addition to soluble complexes, allowing identification and analysis of complexes not accessible in native extracts. We compare the protein complexes detected by HPLC-MS protein correlation profiling in both native and formaldehyde crosslinked U2OS cell extracts. These proteome-wide data sets of both in vivo crosslinked and native protein complexes from U2OS cells are freely available via a searchable online database (www.peptracker.com/epd). Raw data are also available via ProteomeXchange (identifier PXD003754).


Asunto(s)
Reactivos de Enlaces Cruzados/química , Proteínas de la Membrana/metabolismo , Proteómica/métodos , Línea Celular Tumoral , Cromatografía Líquida de Alta Presión/métodos , Humanos , Proteínas de la Membrana/química , Mapas de Interacción de Proteínas , Espectrometría de Masas en Tándem/métodos
9.
Nat Immunol ; 17(1): 104-12, 2016 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-26551880

RESUMEN

We used high-resolution mass spectrometry to map the cytotoxic T lymphocyte (CTL) proteome and the effect of the metabolic checkpoint kinase mTORC1 on CTLs. The CTL proteome was dominated by metabolic regulators and granzymes, and mTORC1 selectively repressed and promoted expression of a subset of CTL proteins (~10%). These included key CTL effector molecules, signaling proteins and a subset of metabolic enzymes. Proteomic data highlighted the potential for negative control of the production of phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3) by mTORC1 in CTLs. mTORC1 repressed PtdIns(3,4,5)P3 production and determined the requirement for mTORC2 in activation of the kinase Akt. Our unbiased proteomic analysis thus provides comprehensive understanding of CTL identity and the control of CTL function by mTORC1.


Asunto(s)
Complejos Multiproteicos/metabolismo , Proteoma/inmunología , Linfocitos T Citotóxicos/metabolismo , Serina-Treonina Quinasas TOR/metabolismo , Animales , Células Cultivadas , Cromatografía , Ensayo de Inmunoadsorción Enzimática , Femenino , Immunoblotting , Masculino , Espectrometría de Masas , Diana Mecanicista del Complejo 1 de la Rapamicina , Ratones , Complejos Multiproteicos/inmunología , Análisis de Secuencia por Matrices de Oligonucleótidos , Linfocitos T Citotóxicos/inmunología , Serina-Treonina Quinasas TOR/inmunología
10.
Proteomics ; 16(3): 381-92, 2016 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-26552604

RESUMEN

Proteomics studies typically analyze proteins at a population level, using extracts prepared from tens of thousands to millions of cells. The resulting measurements correspond to average values across the cell population and can mask considerable variation in protein expression and function between individual cells or organisms. Here, we report the development of micro-proteomics for the analysis of Caenorhabditis elegans, a eukaryote composed of 959 somatic cells and ∼1500 germ cells, measuring the worm proteome at a single organism level to a depth of ∼3000 proteins. This includes detection of proteins across a wide dynamic range of expression levels (>6 orders of magnitude), including many chromatin-associated factors involved in chromosome structure and gene regulation. We apply the micro-proteomics workflow to measure the global proteome response to heat-shock in individual nematodes. This shows variation between individual animals in the magnitude of proteome response following heat-shock, including variable induction of heat-shock proteins. The micro-proteomics pipeline thus facilitates the investigation of stochastic variation in protein expression between individuals within an isogenic population of C. elegans. All data described in this study are available online via the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd), an open access, searchable database resource.


Asunto(s)
Proteínas de Caenorhabditis elegans/análisis , Caenorhabditis elegans/genética , Cromatina/metabolismo , Proteínas de Choque Térmico/análisis , Proteoma/análisis , Proteómica/métodos , Animales , Caenorhabditis elegans/metabolismo , Proteínas de Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/metabolismo , Cromatina/química , Cromatografía de Fase Inversa , Perfilación de la Expresión Génica , Regulación de la Expresión Génica , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/metabolismo , Espectrometría de Masas , Proteoma/genética , Proteoma/metabolismo , Proteómica/estadística & datos numéricos
11.
Mol Cell Proteomics ; 14(7): 1989-2001, 2015 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-25963834

RESUMEN

Periodic starvation of animals induces large shifts in metabolism but may also influence many other cellular systems and can lead to adaption to prolonged starvation conditions. To date, there is limited understanding of how starvation affects gene expression, particularly at the protein level. Here, we have used mass-spectrometry-based quantitative proteomics to identify global changes in the Caenorhabditis elegans proteome due to acute starvation of young adult animals. Measuring changes in the abundance of over 5,000 proteins, we show that acute starvation rapidly alters the levels of hundreds of proteins, many involved in central metabolic pathways, highlighting key regulatory responses. Surprisingly, we also detect changes in the abundance of chromatin-associated proteins, including specific linker histones, histone variants, and histone posttranslational modifications associated with the epigenetic control of gene expression. To maximize community access to these data, they are presented in an online searchable database, the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/).


Asunto(s)
Caenorhabditis elegans/metabolismo , Proteómica/métodos , Estrés Fisiológico , Animales , Proteínas de Caenorhabditis elegans/metabolismo , Cromatina/metabolismo , Histonas/metabolismo , Difusión de la Información , Internet , Metabolismo de los Lípidos , Proteoma/metabolismo
12.
Toxicon ; 56(8): 1350-61, 2010 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-20692275

RESUMEN

The toxigenic freshwater cyanobacterium Cylindrospermopsis raciborskii T3 has been used as a model to study and elucidate the biosynthetic pathway of tetrahydropurine neurotoxins associated with paralytic shellfish poisoning (PSP). There are nevertheless several inconsistencies and contradictions in the toxin profile of this strain as published by different research groups, and claimed to include carbamoyl (STX, NEO, GTX2/3), decarbamoyl (dcSTX), and N-sulfocarbamoyl (C1/2, B1) derivatives. Our analysis of the complete genome of another PSP toxin-producing cyanobacterium, Raphidiopsis brookii D9, which is closely related to C. raciborskii T3, resolved many issues regarding the correlation between biosynthetic pathways, corresponding genes and the T3 toxin profile. The putative sxt gene cluster in R. brookii D9 has a high synteny with the T3 sxt cluster, with 100% nucleotide identity among the shared genes. We also compared the PSP toxin profile of the strains by liquid chromatography coupled to mass spectrometry (LC-MS/MS). In contrast to published reports, our reassessment of the PSP toxin profile of T3 confirmed production of only STX, NEO and dcNEO. We gained significant insights via correlation between specific sxt genes and their role in PSP toxin synthesis in both D9 and T3 strains. In particular, analysis of sulfotransferase functions for SxtN (N-sulfotransferase) and SxtSUL (O-sulfotransferase) enzymes allowed us to propose an extension of the PSP toxin biosynthetic pathway from STX to the production of the derivatives GTX2/3, C1/2 and B1. This is a significantly revised view of the genetic mechanisms underlying synthesis of sulfated and sulfonated STX analogues in toxigenic cyanobacteria.


Asunto(s)
Toxinas Bacterianas/química , Cylindrospermopsis/química , Sulfotransferasas/fisiología , Toxinas Bacterianas/biosíntesis , Toxinas Bacterianas/aislamiento & purificación , Cromatografía Liquida , Cylindrospermopsis/genética , Genes Bacterianos , Genoma Bacteriano , Datos de Secuencia Molecular , Familia de Multigenes , Análisis de Secuencia de ADN , Sulfotransferasas/genética , Espectrometría de Masas en Tándem
13.
PLoS One ; 5(2): e9235, 2010 Feb 16.
Artículo en Inglés | MEDLINE | ID: mdl-20169071

RESUMEN

Cyanobacterial morphology is diverse, ranging from unicellular spheres or rods to multicellular structures such as colonies and filaments. Multicellular species represent an evolutionary strategy to differentiate and compartmentalize certain metabolic functions for reproduction and nitrogen (N(2)) fixation into specialized cell types (e.g. akinetes, heterocysts and diazocytes). Only a few filamentous, differentiated cyanobacterial species, with genome sizes over 5 Mb, have been sequenced. We sequenced the genomes of two strains of closely related filamentous cyanobacterial species to yield further insights into the molecular basis of the traits of N(2) fixation, filament formation and cell differentiation. Cylindrospermopsis raciborskii CS-505 is a cylindrospermopsin-producing strain from Australia, whereas Raphidiopsis brookii D9 from Brazil synthesizes neurotoxins associated with paralytic shellfish poisoning (PSP). Despite their different morphology, toxin composition and disjunct geographical distribution, these strains form a monophyletic group. With genome sizes of approximately 3.9 (CS-505) and 3.2 (D9) Mb, these are the smallest genomes described for free-living filamentous cyanobacteria. We observed remarkable gene order conservation (synteny) between these genomes despite the difference in repetitive element content, which accounts for most of the genome size difference between them. We show here that the strains share a specific set of 2539 genes with >90% average nucleotide identity. The fact that the CS-505 and D9 genomes are small and streamlined compared to those of other filamentous cyanobacterial species and the lack of the ability for heterocyst formation in strain D9 allowed us to define a core set of genes responsible for each trait in filamentous species. We presume that in strain D9 the ability to form proper heterocysts was secondarily lost together with N(2) fixation capacity. Further comparisons to all available cyanobacterial genomes covering almost the entire evolutionary branch revealed a common minimal gene set for each of these cyanobacterial traits.


Asunto(s)
Proteínas Bacterianas/genética , Cianobacterias/genética , Cylindrospermopsis/genética , Genoma Bacteriano/genética , Toxinas Bacterianas/metabolismo , Cianobacterias/clasificación , Cianobacterias/metabolismo , Cylindrospermopsis/citología , Cylindrospermopsis/ultraestructura , Evolución Molecular , Microscopía Electrónica de Transmisión , Familia de Multigenes/genética , Fijación del Nitrógeno/genética , Filogenia , Secuencias Repetitivas de Ácidos Nucleicos/genética , Análisis de Secuencia de ADN , Especificidad de la Especie , Sintenía
14.
Syst Appl Microbiol ; 32(1): 37-48, 2009 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-19118969

RESUMEN

Cylindrospermopsis raciborskii is a species of freshwater, bloom-forming cyanobacterium. C. raciborskii produces toxins, including cylindrospermopsin (hepatotoxin) and saxitoxin (neurotoxin), although non toxin-producing strains are also observed. In spite of differences in toxicity, C. raciborskii strains comprise a monophyletic group, based upon 16S rRNA gene sequence identities (greater than 99%). We performed phylogenetic analyses; 16S rRNA gene and 16S-23S rRNA gene internally transcribed spacer (ITS-1) sequence comparisons, and genomic DNA restriction fragment length polymorphism (RFLP), resolved by pulsed-field gel electrophoresis (PFGE), of strains of C. raciborskii, obtained mainly from the Australian phylogeographic cluster. Our results showed no correlation between toxic phenotype and phylogenetic association in the Australian strains. Analyses of the 16S rRNA gene and the respective ITS-1 sequences (long L, and short S) showed an independent evolution of each ribosomal operon. The genes putatively involved in the cylindrospermopsin biosynthetic pathway were present in one locus and only in the hepatotoxic strains, demonstrating a common genomic organization for these genes and the absence of mutated or inactivated biosynthetic genes in the non toxic strains. In summary, our results support the hypothesis that the genes involved in toxicity may have been transferred as an island by processes of gene lateral transfer, rather than convergent evolution.


Asunto(s)
Cylindrospermopsis/clasificación , Cylindrospermopsis/patogenicidad , Filogenia , Saxitoxina/metabolismo , Uracilo/análogos & derivados , Alcaloides , Toxinas Bacterianas , Cylindrospermopsis/genética , Cylindrospermopsis/fisiología , ADN Bacteriano/análisis , ADN Bacteriano/genética , ADN Espaciador Ribosómico/análisis , ADN Espaciador Ribosómico/genética , Transferencia de Gen Horizontal , Datos de Secuencia Molecular , Péptido Sintasas/genética , Péptido Sintasas/metabolismo , Fenotipo , Sintasas Poliquetidas/genética , Sintasas Poliquetidas/metabolismo , ARN Ribosómico 16S/genética , ARN Ribosómico 23S/genética , Saxitoxina/genética , Análisis de Secuencia de ADN , Especificidad de la Especie , Uracilo/metabolismo
15.
J Bacteriol ; 186(10): 3202-13, 2004 May.
Artículo en Inglés | MEDLINE | ID: mdl-15126483

RESUMEN

The large pathogenicity island (SPI7) of Salmonella enterica serovar Typhi is a 133,477-bp segment of DNA flanked by two 52-bp direct repeats overlapping the pheU (phenylalanyl-tRNA) gene, contains 151 potential open reading frames, and includes the viaB operon involved in the synthesis of Vi antigen. Some clinical isolates of S. enterica serovar Typhi are missing the entire SPI7, due to its precise excision; these strains have lost the ability to produce Vi antigen, are resistant to phage Vi-II, and invade a human epithelial cell line more rapidly. Excision of SPI7 occurs spontaneously in a clinical isolate of S. enterica serovar Typhi when it is grown in the laboratory, leaves an intact copy of the pheU gene at its novel join point, and results in the same three phenotypic consequences. SPI7 is an unstable genetic element, probably an intermediate in the pathway of lateral transfer of such pathogenicity islands among enteric gram-negative bacteria.


Asunto(s)
Antígenos Bacterianos/genética , Polisacáridos Bacterianos/genética , Aminoacil-ARN de Transferencia/genética , Salmonella typhi/genética , Salmonella typhi/patogenicidad , Antígenos Bacterianos/análisis , Secuencia de Bases , Línea Celular , Humanos , Datos de Secuencia Molecular , Operón , Fenotipo , Polisacáridos Bacterianos/análisis , Recombinación Genética
16.
Innovación (Huancayo) ; (3): 109-113, 2002. ilus, tab
Artículo en Español | LIPECS | ID: biblio-1108852

RESUMEN

El objetivo de presentar estos casos es demostrar que en el Hospital El Carmen de Huancayo (3272 m.s.n.m) se realizan técnicas anestésicas diferentes a las tradicionales. Se demuestra que es una técnica sencilla de realizar y segura para los pacientes porque no tiene repercusiones hemodinámicas. Se comentan casos quirúrgicos en los cuales se aplicó con éxito esta técnica. Se sustenta con fotos. Se recomienda a los médicos Anestesiológos emplear esta técnica cuando tengamos la sospecha que acto quirúrgico demorará más de 02 horas y tiene la ventaja de usar el catéter epidural para analgesia postoperatoria en las primeras 24 horas.


Asunto(s)
Femenino , Niño , Humanos , Altitud , Anestesia Caudal
17.
Innovación (Huancayo) ; (3): 103-107, 2002. tab
Artículo en Español | LIPECS | ID: biblio-1108853

RESUMEN

Se reporta una revisión de casos de pacientes operados con Bloqueo de Plexo Braquial realizados en el Hospital El Carmen-Hancayo (3272 m.s.n.m). Entre Junio 1997 y Junio 2001 ingresaron al quirófano 79 pacientes, de los cuales el 86 por ciento fueron del sexo masculino y el 13.92 por ciento del sexo femenino. La edad promedio más frecuentes correspondieron a fracturas de húmero 25.32 por ciento y fracturas de clavícula 21.52 por ciento. Los pacientes fueron intervenidos con Bloqueo del Plexo Braquial técnica Interescalénica. El 59.49 por ciento de bloqueos realizados correspondieron al Plexo Braquial Izquierdo. En el 80 por ciento de casos, no se necesitó sedación complementaria. Se concluye que es una buena alternativa que tienen los Anestesiólogos y es una técnica más barata que la anestesia general.


Asunto(s)
Masculino , Femenino , Niño , Adolescente , Adulto , Persona de Mediana Edad , Anciano , Humanos , Altitud , Anestesia , Bloqueo Nervioso , Plexo Braquial , Epidemiología Descriptiva , Estudios Retrospectivos
18.
Innovación (Huancayo) ; (3): 97-101, 2002. ilus, tab
Artículo en Español | LIPECS | ID: biblio-1108854

RESUMEN

Se revisan casos de pacientes pediátricos operados por el servicio de Traumatología y Ortopedia en el Hospital El Carmen-Huancayo (3272 m.s.n.m). Entre Junio 1997 y Junio 2001, se operaron 23 pacientes pediátricos bajo efectos de Bloqueo Interescalénico del Plexo Braquial, correspondiendo al 14.47 por ciento de los operados. La edad promedio fue 13.47 años, siendo el de menor edad 07 años. El 86.96 por ciento de los pacientes fueron del sexo masculino. Las patologías más frecuentes fueron: fractura de cúbito y radio 26.09 por ciento y fractura de paleta humeral 21.74 por ciento. El 60.87 por ciento de los pacientes necesitó Bloqueo del Plexo Braquial Izquierdo. Se concluye que es una técnica útil y barata.


Asunto(s)
Masculino , Femenino , Niño , Adolescente , Humanos , Altitud , Anestesia , Bloqueo Nervioso , Plexo Braquial , Epidemiología Descriptiva , Estudios Retrospectivos
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