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1.
Med Oral Patol Oral Cir Bucal ; 25(6): e784-e790, 2020 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-33037809

RESUMEN

BACKGROUND: This study investigated the causes of dental implant removal due to complications, and examined whether patients who had dental implant removal desired re-implant prosthesis treatments. MATERIAL AND METHODS: A retrospective case-control study was conducted on patients who had their dental implants removed. We investigated whether the removed dental implant was replaced with other implant prostheses. Age, sex, diabetes, smoking, implant site distribution, reason for implant removal, and blade and root-form implants were categorized as predictive variables. The outcome variable was desire for re-implantation or use of other prosthetic methods after implant removal. A logistic regression model was created to identify patient factors that could predict the re-implantation of dental prostheses after implant removal. RESULTS: A total of 215 dental implants were removed from 143 patients. The most common reason for implant removal was peri-implantitis that was identified in 165 implants. After implant removal, re-implantation was performed in 98 implants (45.6%). Bivariate analyses showed that age, diabetes, implant type, and reason for implant removal were associated with the desire for re-implanted prostheses. The multiple regression model revealed that age, implant type, and reason for implant removal were associated with an increased desire for re-implant prostheses after implant removal. CONCLUSIONS: Re-implantation of prostheses after the removal of dental implants was desired by patients who were younger, had implants placed in the root form, and had implants removed due to prosthetic-related complications.


Asunto(s)
Implantes Dentales , Estudios de Casos y Controles , Implantación Dental Endoósea , Diseño de Prótesis Dental , Prótesis Dental de Soporte Implantado , Fracaso de la Restauración Dental , Estudios de Seguimiento , Humanos , Estudios Retrospectivos
2.
J Laryngol Otol ; 128(10): 926-31, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-25230256

RESUMEN

OBJECTIVE: This study examined whether the occurrence of late neck metastasis in early tongue squamous cell carcinoma can be predicted by evaluating HMGB1 (high mobility group box 1) expression in the primary lesion. METHODS: A case-control study was conducted. The cases comprised 10 patients with late neck metastasis. The controls consisted of 16 patients without recurrence. All were examined immunohistochemically for HMGB1 protein expression. The odds ratio for late neck metastasis in relation to HMGB1 was estimated. RESULTS: RESULTS for HMGB1 were dichotomised into positive staining scores (score, 5-7) and negative scores (0-4). Six cases (60 per cent) and four controls (25 per cent) were HMGB1-positive. Although no significant result was seen, compared with HMGB1-negative patients the odds ratio for late neck metastasis in HMGB1-positive patients was 3.8 (95 per cent confidence interval, 0.6-26.5) after adjusting for other factors. CONCLUSION: In the present study, immunohistochemical study of HMGB1 in early tongue squamous cell carcinoma did not appear to be very useful for predicting occult neck metastasis. Further study is necessary to clarify the relationship between HMGB1 expression and late neck metastasis in early tongue squamous cell carcinoma.


Asunto(s)
Carcinoma de Células Escamosas/metabolismo , Proteína HMGB1/biosíntesis , Neoplasias de la Lengua/metabolismo , Adulto , Anciano , Anciano de 80 o más Años , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patología , Estudios de Casos y Controles , Femenino , Proteína HMGB1/genética , Humanos , Inmunohistoquímica , Ganglios Linfáticos/patología , Metástasis Linfática , Masculino , Persona de Mediana Edad , Neoplasias de la Lengua/genética , Neoplasias de la Lengua/patología
3.
Dentomaxillofac Radiol ; 42(5): 20120265, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23468124

RESUMEN

OBJECTIVES: To evaluate the diagnostic value of MRI for odontogenic tumours. MATERIALS AND METHODS: 51 patients with odontogenic tumours were subjected to pre-operative MRI examinations. For tumours with liquid components, i.e. ameloblastomas and keratocystic odontogenic tumours (KCOTs), the signal intensity (SI) uniformity of their cystic components (UΣ) was calculated and then their UΣ values were compared. For tumours with solid components that had been examined using dynamic contrast-enhanced MRI (DCE-MRI), their CImax (maximum contrast index), Tmax (the time when CImax occurred), CIpeak (CImax × 0.90), Tpeak (the time when CIpeak occurred) and CI300 (i.e. the CI observed at 300 s after contrast medium injection) values were determined from CI curves. We then classified the odontogenic tumours according to their DCE-MRI parameters. RESULTS: Significant differences between the UΣ values of the ameloblastomas and KCOT were observed on T1 weighted images, T2 weighted images and short TI inversion recovery images. Depending on their DCE-MRI parameters, we classified the odontogenic tumours into the following five types: Type A, CIpeak > 2.0 and Tpeak < 200 s; Type B, CIpeak < 2.0 and Tpeak < 200 s; Type C, CI300 > 2.0 and Tmax < 600 s; Type D, CI300 > 2.0 and Tmax > 600 s; Type E, CI300 < 2.0 and Tmax > 600 s. CONCLUSION: Cystic component SI uniformity was found to be useful for differentiating between ameloblastomas and KCOT. However, the DCE-MRI parameters of odontogenic tumours, except for odontogenic fibromas and odontogenic myxomas, contributed little to their differential diagnosis.


Asunto(s)
Neoplasias Maxilomandibulares/patología , Imagen por Resonancia Magnética , Tumores Odontogénicos/patología , Adolescente , Adulto , Anciano , Ameloblastoma/patología , Niño , Medios de Contraste , Líquido Quístico , Diagnóstico Diferencial , Femenino , Fibroma/patología , Humanos , Neoplasias Maxilomandibulares/clasificación , Imagen por Resonancia Magnética/métodos , Imagen por Resonancia Magnética/estadística & datos numéricos , Masculino , Persona de Mediana Edad , Mixoma/patología , Tumores Odontogénicos/clasificación , Curva ROC , Estadísticas no Paramétricas , Adulto Joven
4.
Eur J Med Res ; 16(11): 501-6, 2011 Nov 10.
Artículo en Inglés | MEDLINE | ID: mdl-22027644

RESUMEN

Notch signaling is an evolutionarily conserved mechanism that enables adjacent cells to adopt different fates. Ghost cells (GCs) are anucleate cells with homogeneous pale eosinophilic cytoplasm and very pale to clear central areas (previous nucleus sites). Although GCs are present in a variety of odontogenic lesions notably the calcifying cystic odontogenic tumor (CCOT), their nature and process of formation remains elusive. The aim of this study was to investigate the role of Notch signaling in the cell fate specification of GCs in CCOT. Immunohistochemical staining for four Notch receptors (Notch1, Notch2, Notch3 and Notch4) and three ligands (Jagged1, Jagged2 and Delta1) was performed on archival tissues of five CCOT cases. Level of positivity was quantified as negative (0), mild (+), moderate (2+) and strong (3+). Results revealed that GCs demonstrated overexpression for Notch1 and Jagged1 suggesting that Notch1-Jagged1 signaling might serve as the main transduction mechanism in cell fate decision for GCs in CCOT. Protein localizations were largely membranous and/or cytoplasmic. Mineralized GCs also stained positive implicating that the calcification process might be associated with upregulation of these molecules. The other Notch receptors and ligands were weak to absent in GCs and tumoral epithelium. Stromal endothelium and fibroblasts were stained variably positive.


Asunto(s)
Linaje de la Célula , Quiste Odontogénico Calcificado/metabolismo , Quiste Odontogénico Calcificado/patología , Tumores Odontogénicos/metabolismo , Tumores Odontogénicos/patología , Receptores Notch/metabolismo , Transducción de Señal , Adolescente , Adulto , Femenino , Humanos , Inmunohistoquímica , Ligandos , Masculino , Persona de Mediana Edad , Adulto Joven
5.
Eur J Med Res ; 16(7): 331-4, 2011 Jul 25.
Artículo en Inglés | MEDLINE | ID: mdl-21813375

RESUMEN

Wegener's granulomatosis is a rare multi-system disease characterized by the classic triad of necrotizing granulomas affecting the upper and lower respiratory tracts, disseminated vasculitis and glomerulonephritis. Oral lesions as a presenting feature are only encountered in 2% of these cases. Hyperplastic gingival lesions or strawberry gingivitis, is a characteristic sign of Wegener's granulomatosis. The latter consists of reddish-purple exophytic gingival swellings with petechial haemorrhages thus resembling strawberries. Recognition of this feature is of utmost importance for timely diagnosis and definitive management of this potentially fatal disease. A case of strawberry gingivitis as the first presenting sign of Wegener's granulomatosis affecting a 50-year-old Malay male is reported here. The differential diagnosis of red lesions that may present in the gingiva is discussed.


Asunto(s)
Gingivitis/etiología , Granulomatosis con Poliangitis/diagnóstico , Diagnóstico Diferencial , Granulomatosis con Poliangitis/complicaciones , Granulomatosis con Poliangitis/tratamiento farmacológico , Granulomatosis con Poliangitis/patología , Humanos , Masculino , Persona de Mediana Edad
6.
Eur J Med Res ; 15(10): 456-60, 2010 Oct 25.
Artículo en Inglés | MEDLINE | ID: mdl-21156405

RESUMEN

BACKGROUND: notch receptors are critical determinants of cell fate in a variety of organisms. Notch signaling is involved in the chondrogenic specification of neural crest cells. Aberrant Notch activity has been implicated in numerous human diseases including cancers; however its role in chondrogenic tumors has not been clarified. METHOD: tissue samples from a case of primary chondrosarcoma of the maxilla and its recurrent tumor were examined immunohistochemically for Notch1-4 and their ligands (Jagged1, Jagged2 and Delta1) expression. RESULTS: both primary and recurrent tumors were histopathologically diagnosed as conventional hyaline chondrosarcoma (WHO Grade I). Hypercellular tumor areas strongly expressed Notch3 and Jagged1 in spindle and pleomorphic cells suggesting up-regulation of these protein molecules at sites of tumor proliferation. Expression patterns were distinct with some overlap. Differentiated malignant and atypical chondrocytes demonstrated variable expression levels of Jagged1, and weak to absent staining for Notch1, 4 and Delta1. Protein immunolocalization was largely membranous and cytoplasmic, sometimes outlining the lacunae of malignant chondrocytes. Hyaline cartilage demonstrated a diffuse or granular precipitation of Jagged1 suggesting presence of soluble Jagged1 activity at sites of abnormal chondrogenesis. No immunoreactivity for the other Notch members was observed. Calcified cartilage was consistently Notch-negative indicating down-regulation of Notch with cartilage maturation. Stromal components namely endothelial cells and fibroblasts variably expressed Notch1, 3 and Jagged1 but were mildly or non-reactive for the other members. CONCLUSIONS: Results indicate that Notch signaling pathway may participate in cellular differentiation and proliferation in chondrosarcoma. Findings implicate Notch3 and Jagged1 as key molecules that influence the differentiation and maturation of cells of chondrogenic lineage.


Asunto(s)
Condrosarcoma/metabolismo , Receptores Notch/genética , Cartílago/metabolismo , Cartílago/patología , Condrosarcoma/patología , Femenino , Humanos , Inmunohistoquímica , Neoplasias Maxilares/metabolismo , Neoplasias Maxilares/patología , Persona de Mediana Edad , Neoplasias Nasofaríngeas/metabolismo , Neoplasias Nasofaríngeas/radioterapia , Recurrencia Local de Neoplasia/metabolismo , Proteínas Proto-Oncogénicas/metabolismo , Receptor Notch1/metabolismo , Receptor Notch2/metabolismo , Receptor Notch3 , Receptor Notch4 , Receptores Notch/metabolismo
7.
Eur J Med Res ; 15(4): 180-4, 2010 Apr 08.
Artículo en Inglés | MEDLINE | ID: mdl-20554499

RESUMEN

BACKGROUND: Squamous odontogenic tumor (SOT) is a rare benign odontogenic epithelial neoplasm. A slow-growing painless expansive swelling is the common presenting symptom. Histopathologically, SOT can be easily misdiagnosed as an acanthomatous ameloblastoma. Although Notch receptors and ligands have been shown to play a role in cell fate decisions in ameloblastomas, the role of these cell signaling molecules in SOT is unknown. CASE REPORT: This paper describes a case of SOT affecting the anterior mandible of a 10-year-old Indian female. The patient was treated by local surgical excision and there has been no follow-up clinical record of recurrence 5 years after primary treatment. Histo?pathological examination revealed a solid, locally-infiltrative neoplasm composed of bland-looking squamatoid islands scattered in a mature fibrous connective tissue stroma and the diagnosis was SOT. Immunohistochemical evaluation showed positive reactivity of varying intensity in the neoplastic epithelial cells for Notch1, Notch3, Notch4, and their ligands Jagged1 and Delta1. Expression patterns showed considerable overlap. No immunoreactivity was detected for Notch2 and Jagged2. CONCLUSIONS: Present findings suggest that Notch receptors and their ligands play differential roles in the cytodifferentiation of SOT.


Asunto(s)
Proteínas de Unión al Calcio/metabolismo , Péptidos y Proteínas de Señalización Intercelular/metabolismo , Neoplasias Mandibulares/genética , Proteínas de la Membrana/metabolismo , Tumor Odontogénico Escamoso/genética , Proteínas Proto-Oncogénicas/metabolismo , Receptor Notch1/metabolismo , Receptores Notch/metabolismo , Niño , Tejido Conectivo/patología , Femenino , Humanos , Péptidos y Proteínas de Señalización Intracelular , Proteína Jagged-1 , Neoplasias Mandibulares/metabolismo , Neoplasias Mandibulares/patología , Neoplasias Mandibulares/cirugía , Tumor Odontogénico Escamoso/metabolismo , Tumor Odontogénico Escamoso/patología , Tumor Odontogénico Escamoso/cirugía , Receptor Notch3 , Receptor Notch4 , Proteínas Serrate-Jagged
8.
Eur J Med Res ; 15(3): 135-8, 2010 Mar 30.
Artículo en Inglés | MEDLINE | ID: mdl-20452900

RESUMEN

The purpose of this report is to document a case of unsuspected ameloblastoma involving the right man dibular subpontic region in a 38-year-old Cambodian female patient. This lesion was purportedly preceded by multiple radiolucencies which were diagnosed as radicular cysts and treated a few times in the past years by enucleation followed by endodontic therapy of the affected teeth. Bridgework restoration of the partially edentulous area was performed. This case report demonstrates radiographic changes that occurred in the periods before and after the diagnosis of ameloblastoma. The case may represent an example of radicular cysts and ameloblastoma occurring as a collision phenomenon, or the ameloblastoma may have arisen as a result of neoplastic transformation of the lining epithelium in an inflammatory odontogenic epithelial cyst.


Asunto(s)
Ameloblastoma/patología , Neoplasias Mandibulares/patología , Quiste Radicular/diagnóstico , Radiografía Dental/efectos adversos , Adulto , Ameloblastoma/etiología , Ameloblastoma/cirugía , Femenino , Estudios de Seguimiento , Humanos , Neoplasias Mandibulares/etiología , Neoplasias Mandibulares/cirugía , Quiste Radicular/terapia , Resultado del Tratamiento
9.
Eur J Med Res ; 14(7): 315-9, 2009 Jul 22.
Artículo en Inglés | MEDLINE | ID: mdl-19661015

RESUMEN

STATEMENT OF THE PROBLEM: Dysplasia, the morphological yardstick of epithelial precursor lesions, is the collective term for a variety of architectural and cytological changes within the altered oral epithelium. Angiogenic squamous dysplasia (ASD), a distinct morphological characteristic in pre-invasive bronchial lesions, describes the presence of capillary tufts that are closely juxtaposed to and projecting into the dysplastic bronchial epithelium. OBJECTIVE: To determine whether ASD-like phenomenon occurs in oral epithelial precursor lesions, and to speculate on its relevance. METHODS: Twenty cases each of mild, moderate and severe oral dysplasia (inclusive of carcinoma-in-situ), and 10 normal oral mucosa (normal controls) were serial sectioned for H and E staining, and for microvessel density (MVD) scoring with CD31, CD34 and CD105. Microcapillary pattern images were digitally captured for 3-D reconstruction. RESULTS: Oral ASD foci consisting of CD31- and CD34-positive capillary loops abutting onto the overlying dysplastic oral epithelium (and causing it to assume an irregular or papillary surface configuration) were identified in moderate (3/20; 15%) and severe dysplasia (13/20; 65%), but not in normal oral mucosa and mild dysplasia. MVD score demonstrated increasing vascularity as epithelium progressed from normal to severe dysplasia (p<0.05). CD105 demonstrated increase neovascularization in all dysplasia grades (p<0.05). CONCLUSIONS: These preliminary findings taken together suggest that: 1. ASD-like phenomenon may be an important intermediary biomarker in oral precursor lesions; and 2. architectural alterations of the entire disturbed mucosa may be a more useful pre-malignancy index.


Asunto(s)
Carcinoma de Células Escamosas/irrigación sanguínea , Mucosa Bucal/irrigación sanguínea , Lesiones Precancerosas/irrigación sanguínea , Antígenos CD/análisis , Antígenos CD34/análisis , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patología , Endoglina , Humanos , Inmunohistoquímica , Mucosa Bucal/metabolismo , Mucosa Bucal/patología , Neovascularización Patológica , Molécula-1 de Adhesión Celular Endotelial de Plaqueta/análisis , Lesiones Precancerosas/metabolismo , Lesiones Precancerosas/patología , Receptores de Superficie Celular/análisis
10.
Biocell ; 32(3): 251-258, Dec. 2008. ilus
Artículo en Inglés | LILACS | ID: lil-541107

RESUMEN

The Notch signaling pathway is an evolutionary conserved mechanism that plays an important role in cell-cell communication and cell fate in a wide range of tissues. The mammalian family of Notch receptors consists of 4 members: Notch1/2/3/4. The Notch ligand family consists of 5 members: Delta1/3/4 and Jagged1/2. Math1 encodes a murine Basic helix-loop-helix (bHLH) transcription factor that acts as positive regulator of cell differentiation. Recently, links between Notch and Math1 pathways were demonstrated in various tissues. Expression of Notch1, Jagged2 and Math1 were analyzed in the mouse molar tooth germ during embryonic stage (E) 13 and E15 and during postnatal stage (PN) 1, PN3, PN5, PN10 and PN14 by using in situ hybridization. Positive Notch1 expression was found at the tooth bud during embryonic stages, but its expression was absent from the basal cells in contact with the dental mesenchyme. Jagged2 and Math1 were strongly expressed in differentiated ameloblasts and odontoblasts and Math1 strong expression was even maintained until PN14 stage. Math1 showed the strongest expression. Our results suggest that the Notch1 signaling pathway through Jagged2 could be importantly related to Math1, directing the process of odontogenesis toward cell differentiation.


Asunto(s)
Animales , Ratas , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico , Regulación del Desarrollo de la Expresión Génica , Germen Dentario/citología , Germen Dentario/fisiología , Ratones Endogámicos BALB C , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Transducción de Señal/fisiología , Diente Molar/anatomía & histología , Diente Molar/fisiología , Odontogénesis/fisiología , Receptor Notch1/genética , Receptor Notch1/metabolismo
11.
Biocell ; 32(3): 251-258, Dec. 2008. ilus
Artículo en Inglés | BINACIS | ID: bin-127199

RESUMEN

The Notch signaling pathway is an evolutionary conserved mechanism that plays an important role in cell-cell communication and cell fate in a wide range of tissues. The mammalian family of Notch receptors consists of 4 members: Notch1/2/3/4. The Notch ligand family consists of 5 members: Delta1/3/4 and Jagged1/2. Math1 encodes a murine Basic helix-loop-helix (bHLH) transcription factor that acts as positive regulator of cell differentiation. Recently, links between Notch and Math1 pathways were demonstrated in various tissues. Expression of Notch1, Jagged2 and Math1 were analyzed in the mouse molar tooth germ during embryonic stage (E) 13 and E15 and during postnatal stage (PN) 1, PN3, PN5, PN10 and PN14 by using in situ hybridization. Positive Notch1 expression was found at the tooth bud during embryonic stages, but its expression was absent from the basal cells in contact with the dental mesenchyme. Jagged2 and Math1 were strongly expressed in differentiated ameloblasts and odontoblasts and Math1 strong expression was even maintained until PN14 stage. Math1 showed the strongest expression. Our results suggest that the Notch1 signaling pathway through Jagged2 could be importantly related to Math1, directing the process of odontogenesis toward cell differentiation.(AU)


Asunto(s)
Animales , Ratas , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/genética , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/metabolismo , Regulación del Desarrollo de la Expresión Génica , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Transducción de Señal/fisiología , Germen Dentario/citología , Germen Dentario/fisiología , Ratones Endogámicos BALB C , Diente Molar/anatomía & histología , Diente Molar/fisiología , Odontogénesis/fisiología , Receptor Notch1/genética , Receptor Notch1/metabolismo
12.
Eur J Med Res ; 13(10): 476-80, 2008 Oct 27.
Artículo en Inglés | MEDLINE | ID: mdl-19008176

RESUMEN

BACKGROUND: In general, Notch is a representative signal which controls morphosis and differentiation of cells, but its role in human odontogenic neoplasms, especially in ameloblastoma and its malignant counterpart, ameloblastic carcinoma, is not known. METHODS: We examined Notch1 peptide and its gene (mRNA) in an ameloblastoma (case 1: 27-year-old female, right mandibular tumor) and an ameloblastic carcinoma (case 2: 93-year-old female, right mandibular tumor), using immunohistochemistry (IHC) and in situ hybridization (ISH) techniques. RESULTS: Notch1 intracellular domain (NICD) positive products were observed in the cells at the peripheral layer of most proliferating epithelial tumor nests in case 1. In case 2, positive products were similarly detected. In particular, small numbers of mitoses were identified in the nuclear region with intense NICD positive reaction. CONCLUSIONS: Notch signaling plays some role in cytological differentiation or acquisition of tissue specific characteristics in neoplastic cells of odontogenic neoplasms, including ameloblastoma and ameloblastic carcinoma. Notch1 may also contribute to cell cycle arrest induced by Notch1 activation in ameloblastic carcinoma.


Asunto(s)
Ameloblastoma/genética , Ameloblastoma/metabolismo , Neoplasias Mandibulares/genética , Neoplasias Mandibulares/metabolismo , Receptor Notch1/genética , Receptor Notch1/metabolismo , Adulto , Anciano , Anciano de 80 o más Años , Ameloblastoma/patología , Femenino , Expresión Génica , Humanos , Inmunohistoquímica , Hibridación in Situ , Neoplasias Mandibulares/patología , Estructura Terciaria de Proteína , ARN Mensajero/genética , ARN Mensajero/metabolismo , ARN Neoplásico/genética , ARN Neoplásico/metabolismo , Receptor Notch1/química
13.
Histopathology ; 53(4): 458-67, 2008 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-18983611

RESUMEN

AIMS: To study the stromal variation and role of stromal-tumour cell interaction in impaired bone formation as well as enhanced bone resorption in ameloblastoma. METHODS AND RESULTS: Four types of stroma were observed histologically; fibrous, desmoplastic, myxoid and myxoid with hyalinization. Osteoblast and osteoclast were counted using haematoxylin and eosin sections and immunohistochemistry with CD68. After histomorphometric analysis, only fibrous and myxoid types of stroma were distinctly identified. Secreted frizzled-related peptide (sFRP)-2, transforming growth factor-beta 1 and receptor activator of nuclear factor-kappaB ligand (RANKL) revealed strong expression in myxoid type compared with the normal stroma. Bone morphogenetic protein (BMP)-2 was negative in myxoid type, but positive in normal stroma. Fibrous-type stroma showed weak expression of all antigens except RANKL compared with myxoid type. CONCLUSIONS: The results suggest that stroma does not act only in bone resorption, but also in the suppression of new bone formation. sFRP-2 is the main factor for impaired bone formation. The expression of markers related to osteoclastogenesis and suppression of osteoblast formation is higher in myxoid-type than in fibrous-type stroma. Tumour cells create a favourable environment for impaired bone formation by secreting sFRP-2 as well as bone resorption by secreting RANKL and interleukin-6.


Asunto(s)
Ameloblastoma/patología , Huesos/metabolismo , Neoplasias Maxilomandibulares/patología , Osteogénesis/fisiología , Células del Estroma/metabolismo , Adulto , Ameloblastoma/metabolismo , Antígenos CD/metabolismo , Antígenos de Diferenciación Mielomonocítica/metabolismo , Neoplasias Óseas/metabolismo , Neoplasias Óseas/patología , Resorción Ósea/metabolismo , Resorción Ósea/patología , Huesos/patología , Femenino , Humanos , Interleucina-6/metabolismo , Neoplasias Maxilomandibulares/metabolismo , Masculino , Proteínas de la Membrana/metabolismo , Persona de Mediana Edad , Invasividad Neoplásica , Osteoblastos/metabolismo , Osteoclastos/metabolismo , Osteoclastos/patología , Ligando RANK/metabolismo , Células del Estroma/patología , Células Tumorales Cultivadas
14.
Oral Dis ; 14(7): 652-7, 2008 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-18627502

RESUMEN

OBJECTIVES AND DESIGN: The expressions of human beta defensin-1 (HBD-1), -2 (HBD-2) and -3 (HBD-3) in non-inflamed pseudocysts such as mucoceles were investigated immunohistochemically in this study. MATERIALS AND METHODS: Mucocele specimens were obtained from 21 patients. The expression of HBDs was studied immunohistochemically by using antibodies directed against HBD-1, -2, and -3. Statistical analyses were carried out on serial sections stained with antibodies. RESULTS: Cells expressing HBDs were found in mucoceles. The expression of HBD-2 was observed in floating cells in all the specimens, whereas HBD-1 and HBD-3-expressing cells were detected in 93% and 73% of the mucoceles, respectively. The HBD-2 signal was the most intense and the HBD-3 signal intensity was weaker than that of HBD-1. HBDs were expressed in neutrophils and in other floating cells. Interestingly, the signal intensity and the population of positive cells located close to the centers of cysts were higher than those located in the peripheral areas of cysts. CONCLUSION: The expression of HBDs was found even in non-inflamed pseudocysts such as mucoceles. These results suggest that an unknown mechanism not involved in biophylaxis for the expression of HBDs may exist.


Asunto(s)
Enfermedades de los Labios/metabolismo , Mucocele/metabolismo , beta-Defensinas/biosíntesis , Adolescente , Adulto , Niño , Preescolar , Femenino , Expresión Génica , Humanos , Inmunohistoquímica , Masculino , Adulto Joven
15.
Neoplasma ; 55(4): 299-304, 2008.
Artículo en Inglés | MEDLINE | ID: mdl-18505340

RESUMEN

Head and neck squamous cell carcinoma (HNSCC) is a diverse group of cancers that are frequently aggressive in their biologic behavior. Inactivation of tumor suppressor gene (TSG) is one of the most critical steps leading to HNSCC. Loss of heterozygosity analysis is very sensitive method for the detection of frequent allelic loss in a chromosomal locus. This method has been considered as an important evidence for the localization of TSGs. We analyzed loss of heterozygosity (LOH) at chromosome 4q22-35 region by using 14 polymorphic microsatellite markers in 83 matched normal and HNSCC tissues. LOH was detected at least in one location in 71 of 83 (86%) tumor tissues. Frequent deletions were detected at the location of microsatellite markers, D4S2909 (46%), D4S2623 (51%), D4S406 (48%), D4S1644 (45%) and D4S2979 (40%). Four different frequently deleted regions at 4q22, 4q25, 4q31 and 4q34-35 were observed. These regions include several putative TSGs such as Caspase-6, SMARCAD1, SMARCA5, SAP30 and ING2. Further molecular analysis of each gene should be performed to clarify their roles in head and neck squamous cell carcinogenesis.


Asunto(s)
Carcinoma de Células Escamosas/genética , Deleción Cromosómica , Cromosomas Humanos Par 4 , Neoplasias de Cabeza y Cuello/genética , Pérdida de Heterocigocidad , Mapeo Cromosómico , Humanos , Repeticiones de Microsatélite
16.
Eur J Med Res ; 13(1): 1-3, 2008 Jan 23.
Artículo en Inglés | MEDLINE | ID: mdl-18226989

RESUMEN

Expression pattern of Jagged2 gene in mandibular condylar cartilage was examined by means of in situ hybridization (ISH) technique. At E14, Jagged2 mRNA signals appeared in cytoplasm of proliferating chondrocytes. From E15 to E19, Jagged2 mRNA was detected throughout almost all cytoplasm in all layers. However, the distribution pattern was not uniform. These results suggest that Jagged2 plays an essential role for mandibular condylar cartilage morphogenesis and development.


Asunto(s)
Cartílago/embriología , Expresión Génica , Cóndilo Mandibular/embriología , Proteínas de la Membrana/genética , Animales , Cartílago/metabolismo , Condrocitos/citología , Condrocitos/metabolismo , Citoplasma/metabolismo , Hibridación in Situ , Proteína Jagged-2 , Cóndilo Mandibular/metabolismo , Ratones , Ratones Endogámicos , Osteopontina/genética
17.
Folia Biol (Praha) ; 54(5): 157-61, 2008.
Artículo en Inglés | MEDLINE | ID: mdl-19178815

RESUMEN

B-RAF is one of the most commonly mutated oncogenes in human cancer. However, the mutation status of B-RAF has not been established completely in HNSCC. We have analysed the mutation status of the kinase domain of the B-RAF gene (exons 11 and 15) in 91 Japanese HNSCC patients as well as 12 HNSCC cell lines. DNA was extracted and amplified by PCR. Mutations were then analysed by SSCP mutation detection method. Since V600EB-RAF constitutes 90 % of the mutations identified in B-RAF in human cancers, we also used MASA analysis to specifically detect this mutation in exon 15 of B-RAF. Using both methods, no mutation was found in both exon 11 and 15 in all patients and cell lines. Mu tations are absent or rare in the kinase domain of B-RAF in Japanese HNSCC. However, more studies are still needed to determine its usefulness as a target for molecular therapy in these patients.


Asunto(s)
Carcinoma de Células Escamosas/genética , Neoplasias de Cabeza y Cuello/genética , Mutación/genética , Proteínas Proto-Oncogénicas B-raf/genética , Alelos , Línea Celular Tumoral , Análisis Mutacional de ADN , Exones/genética , Humanos , Reacción en Cadena de la Polimerasa , Polimorfismo Conformacional Retorcido-Simple
18.
Eur J Med Res ; 12(10): 515-9, 2007 Oct 30.
Artículo en Inglés | MEDLINE | ID: mdl-18024259

RESUMEN

OBJECTIVE: The purpose of this study was to investigate the expression pattern of Notch signaling in mandibular condylar cartilage, as a type of secondary cartilage. METHODS: Mandibular condyle of ddY mice were fixed from embryonic day 14 (E14) through just after birth (equivalent to E19). Samples were cut into 4 mum serial sections through the central area of the mandibular condyle at the sagittal plane. Serial sections were examined using histological, immunohistochemical (IHC) and in situ hybridization (ISH) techniques. RESULTS: At E14, there were no developmental features of mandibular condyle. At the distal upper portion of developmental mandibular bone, mesenchymal cell proliferation and condensation without metacholomatic reaction to toluidine blue (TB) were seen. At E15, mandibular condylar cartilage was clearly evident, as TB metacholomasia. In IHC specimens at E14, expression of Notch1 intracellular domain (NICD) was observed in the nuclei of coagulating mesenchymal cells. After E15, NICD appeared in the nuclei and the cytoplasms of cells. In ISH examination at E14, expressions of Notch1 mRNA appeared in cytoplasm of proliferating chondrocytes. From E15 to E19, Notch1 mRNA was detected throughout almost all cytoplasm in all layers. CONCLUSION: These IHC and ISH results suggest that Notch signaling plays an essential role for mandibular condylar cartilage morphogenesis and development.


Asunto(s)
Cartílago Articular , Cóndilo Mandibular , Receptor Notch1/metabolismo , Transducción de Señal/fisiología , Animales , Cartílago Articular/citología , Cartílago Articular/embriología , Cartílago Articular/metabolismo , Femenino , Hibridación in Situ , Cóndilo Mandibular/anatomía & histología , Cóndilo Mandibular/embriología , Ratones , Embarazo , Receptor Notch1/genética
19.
Int J Oral Maxillofac Surg ; 35(4): 378-82, 2006 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-16360304

RESUMEN

Renal osteodystrophy (ROD) is one of the most common complications affecting patients with chronic renal failure both before and after the initiation of maintenance dialysis, but macrognathia secondary to ROD is rare. Usually, enlarged jaws due to ROD do not return to their normal contours after the treatment of hyperparathyroidism. To the authors' knowledge, this article describes the second case of macrognathia secondary to dialysis-related ROD treated successfully by parathyroidectomy. Immunohistochemical study of the maxilla confirmed that parathyroidectomy could stop maladaptive parathyroid hormone stimulation, which leads not only to the formation of osteoblastic progenitors that become fibroblast-like cells but also to osteoclast formation.


Asunto(s)
Trastorno Mineral y Óseo Asociado a la Enfermedad Renal Crónica/complicaciones , Enfermedades Maxilomandibulares/cirugía , Fallo Renal Crónico/complicaciones , Paratiroidectomía , Femenino , Humanos , Enfermedades Maxilomandibulares/diagnóstico por imagen , Enfermedades Maxilomandibulares/etiología , Fallo Renal Crónico/terapia , Maxilar/diagnóstico por imagen , Maxilar/patología , Maxilar/cirugía , Enfermedades Maxilares/diagnóstico por imagen , Enfermedades Maxilares/cirugía , Persona de Mediana Edad , Radiografía , Diálisis Renal/efectos adversos
20.
Eur J Med Res ; 10(11): 475-9, 2005 Nov 16.
Artículo en Inglés | MEDLINE | ID: mdl-16354601

RESUMEN

In this immunohistochemical examination, the expression of Runx2, Notch1, Delta and Osteopontin peptides were detected in neoplastic cells in 10 Japanese cases of osteosarcoma. Immunohistochemically, Runx2 peptide expression appeared in the cytoplasm of almost all neoplastic cells of the 10 cases examined. However, Notch1 peptide expression appeared in the cytoplasm of neoplastic cells in the localized and comparatively well-differentiated area of osteosarcoma, which osteoblastic and chondroblastic containing osteoid and/or chondroid tissues. No expression of Notch1 peptide was detected in the fibroblastic and poorly differentiated areas. Delta peptide appearance was nearly the same pattern of Notch1 peptide. Expression of Osteopontin peptide appeared in almost all cells and the strength expression was shown in the area of comparatively well-differentiated tissues. Therefore, these results suggest that Runx2, Notch1, and Delta peptides are closely related to cytological differentiation or acquisition of tissue specific characteristics in neoplastic cells in osteosarcomas.


Asunto(s)
Neoplasias Óseas/patología , Diferenciación Celular , Inmunohistoquímica , Osteosarcoma/patología , Adolescente , Neoplasias Óseas/metabolismo , Niño , Subunidad alfa 1 del Factor de Unión al Sitio Principal/metabolismo , Citoplasma/metabolismo , Femenino , Humanos , Lactante , Ligandos , Masculino , Persona de Mediana Edad , Osteopontina , Osteosarcoma/metabolismo , Receptor Notch1/metabolismo , Sialoglicoproteínas/metabolismo
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