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1.
Nature ; 590(7847): 561-565, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33627814

RESUMEN

The fundamental building blocks of the proton-quarks and gluons-have been known for decades. However, we still have an incomplete theoretical and experimental understanding of how these particles and their dynamics give rise to the quantum bound state of the proton and its physical properties, such as its spin1. The two up quarks and the single down quark that comprise the proton in the simplest picture account only for a few per cent of the proton mass, the bulk of which is in the form of quark kinetic and potential energy and gluon energy from the strong force2. An essential feature of this force, as described by quantum chromodynamics, is its ability to create matter-antimatter quark pairs inside the proton that exist only for a very short time. Their fleeting existence makes the antimatter quarks within protons difficult to study, but their existence is discernible in reactions in which a matter-antimatter quark pair annihilates. In this picture of quark-antiquark creation by the strong force, the probability distributions as a function of momentum for the presence of up and down antimatter quarks should be nearly identical, given that their masses are very similar and small compared to the mass of the proton3. Here we provide evidence from muon pair production measurements that these distributions are considerably different, with more abundant down antimatter quarks than up antimatter quarks over a wide range of momenta. These results are expected to revive interest in several proposed mechanisms for the origin of this antimatter asymmetry in the proton that had been disfavoured by previous results4, and point to future measurements that can distinguish between these mechanisms.

2.
Med Oral Patol Oral Cir Bucal ; 25(6): e784-e790, 2020 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-33037809

RESUMEN

BACKGROUND: This study investigated the causes of dental implant removal due to complications, and examined whether patients who had dental implant removal desired re-implant prosthesis treatments. MATERIAL AND METHODS: A retrospective case-control study was conducted on patients who had their dental implants removed. We investigated whether the removed dental implant was replaced with other implant prostheses. Age, sex, diabetes, smoking, implant site distribution, reason for implant removal, and blade and root-form implants were categorized as predictive variables. The outcome variable was desire for re-implantation or use of other prosthetic methods after implant removal. A logistic regression model was created to identify patient factors that could predict the re-implantation of dental prostheses after implant removal. RESULTS: A total of 215 dental implants were removed from 143 patients. The most common reason for implant removal was peri-implantitis that was identified in 165 implants. After implant removal, re-implantation was performed in 98 implants (45.6%). Bivariate analyses showed that age, diabetes, implant type, and reason for implant removal were associated with the desire for re-implanted prostheses. The multiple regression model revealed that age, implant type, and reason for implant removal were associated with an increased desire for re-implant prostheses after implant removal. CONCLUSIONS: Re-implantation of prostheses after the removal of dental implants was desired by patients who were younger, had implants placed in the root form, and had implants removed due to prosthetic-related complications.


Asunto(s)
Implantes Dentales , Estudios de Casos y Controles , Implantación Dental Endoósea , Diseño de Prótesis Dental , Prótesis Dental de Soporte Implantado , Fracaso de la Restauración Dental , Estudios de Seguimiento , Humanos , Estudios Retrospectivos
3.
Inorg Chem ; 59(17): 12913-12919, 2020 Sep 08.
Artículo en Inglés | MEDLINE | ID: mdl-32811139

RESUMEN

Low-temperature reaction of A-site-ordered layered perovskite Eu2SrFe2O7 (T structure) with CaH2 induces a shift in the Eu2O2 slabs to form Eu2SrFe2O6 with a T' structure (I4/mmm space group) in which only the Fe cation is reduced. Contrary to the previously reported T' structures with Jahn-Teller-active d9 cations (Cu2+ and Ni+), stabilization of Eu2SrFe2O6 with the Fe2+ (d6) cation reflects the stability of the FeO4 square-planar unit. The stability of T'-type Eu2SrFe2O6 over a T-type polymorph is confirmed by density functional theory calculations, revealing the dz2 occupancy for the T' structure. Eu2SrFe2O6 has a bilayer magnetic framework with an Fe-O-Fe superexchange J∥ and an Fe-Fe direct exchange J⊥ (where J∥ > J⊥), which broadly explains the observed TN of 390-404 K. Interestingly, the magnetic moments of Eu2SrFe2O6 lie in the ab plane, in contrast to the structurally similar Sr3Fe2O4Cl2 having an out-of-plane spin alignment.

4.
Br J Surg ; 106(5): 616-625, 2019 04.
Artículo en Inglés | MEDLINE | ID: mdl-30725479

RESUMEN

BACKGROUND: Visceral obesity is one of the risk factors for clinically relevant pancreatic fistula after pancreatic resection. The objective of this study was to evaluate the impact of intraperitoneal lipolysis on postoperative pancreatic fistula. METHODS: The degree of intraperitoneal lipolysis was investigated by measuring the free fatty acid concentration in drain discharge in patients after pancreatic resection. An experimental pancreatic fistula model was prepared by pancreatic transection, and the impact of intraperitoneal lipolysis was evaluated by intraperitoneal administration of triolein (triglyceride) with, or without orlistat (lipase inhibitor). RESULTS: Thirty-three patients were included in the analysis. The free fatty acid concentration in drain discharge on postoperative day 1 was significantly associated with the development of a clinically relevant pancreatic fistula (P = 0·004). A higher free fatty acid concentration in drain discharge was associated with more visceral adipose tissue (P = 0·009). In the experimental model that included 98 rats, intraperitoneal lipolysis caused an increased amount of pancreatic juice leakage and multiple organ dysfunction. Intraperitoneal administration of a lipase inhibitor reduced lipolysis and prevented deterioration of the fistula. CONCLUSION: Intraperitoneal lipolysis significantly exacerbates pancreatic fistula after pancreatic resection. Inhibition of lipolysis by intraperitoneal administration of a lipase inhibitor could be a promising therapy to reduce clinically relevant postoperative pancreatic fistula. Surgical relevance Clinically, there are two types of pancreatic fistula after pancreatic resections: harmless biochemical leak and harmful clinically relevant pancreatic fistula. Visceral obesity is one of the known risk factors for clinically relevant pancreatic fistula; however, the underlying mechanisms remained to be elucidated. Patients with clinically relevant pancreatic fistula had a higher free fatty acid concentration in the drain discharge, suggesting a relationship between intraperitoneal lipolysis and pancreatic fistula. The experimental model of pancreatic fistula demonstrated that intraperitoneal lipolysis caused deterioration in pancreatic fistula, suggesting that intraperitoneal lipolysis is one of the mechanisms that drives biochemical leakage to clinically relevant pancreatic fistula. Intraperitoneal administration of a lipase inhibitor prevented lipolysis as well as pancreatic fistula deterioration in the experimental model, suggesting a future clinical application for lipase inhibitors in prevention of clinically relevant pancreatic fistula.


Asunto(s)
Grasa Intraabdominal/fisiopatología , Lipólisis/fisiología , Pancreatectomía/efectos adversos , Fístula Pancreática/etiología , Pancreaticoduodenectomía/efectos adversos , Anciano , Animales , Modelos Animales de Enfermedad , Ácidos Grasos no Esterificados/análisis , Femenino , Humanos , Lipasa/antagonistas & inhibidores , Lipólisis/efectos de los fármacos , Masculino , Persona de Mediana Edad , Obesidad Abdominal/complicaciones , Obesidad Abdominal/fisiopatología , Fístula Pancreática/prevención & control , Jugo Pancreático/fisiología , Complicaciones Posoperatorias/fisiopatología , Ratas Sprague-Dawley , Factores de Riesgo
5.
Lupus ; 27(7): 1202-1206, 2018 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-29523055

RESUMEN

It has been reported that T helper 17 cells are involved in the pathogenesis of systemic lupus erythematosus, but there is no report on interleukin-17-targeted therapy. We report a case of a 62-year-old female who presented with psoriasis vulgaris and refractory lupus nephritis. Because her conditions were resistant to conventional treatment, and flow cytometry confirmed the proliferation of activated T helper 17 cells in peripheral blood, and examination of a renal biopsy tissue sample confirmed infiltration of numerous interleukin-17-positive lymphocytes to the renal interstitium, administration of the anti-interleukin-17A antibody secukinumab was initiated. After starting secukinumab the clinical and biological features were improved.


Asunto(s)
Anticuerpos Monoclonales/uso terapéutico , Interleucina-17/antagonistas & inhibidores , Nefritis Lúpica/complicaciones , Psoriasis/tratamiento farmacológico , Anticuerpos Monoclonales Humanizados , Femenino , Humanos , Interleucina-17/sangre , Persona de Mediana Edad
6.
Lupus ; 27(5): 802-811, 2018 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-29308726

RESUMEN

Objective We examined the efficacy and safety of rituximab in patients with refractory systemic lupus erythematosus (SLE). Methods The study enrolled 63 SLE patients who were treated with rituximab between 2002 and 2015. The participants underwent a battery of tests before treatment and at one year. Treatment ranged from two to four times at 500 or 1000 mg. Results Baseline characteristics were males:females = 6:57, age 33.9 years, and disease duration 87.2 months. The primary endpoint: The rate of major clinical response (MCR) was 60% while the partial clinical response (PCR) was 25%. Thirty of 36 (83%) patients with lupus nephritis (WHO II: 2, III: 5, IV: 22, V: 4, IV+V: 2, not assessed: 1) and 22 of 24 patients (92%) with neuropsychiatric SLE, who could be followed at one year, showed changes from BILAG A or B score to C or D score at one year. Multivariate analysis identified high anti-dsDNA antibody and shorter disease duration as significant determinants of MCR at one year. Repeat examination was conducted at five years. Primary failure was recorded in 8.8% and secondary failure in 32.4% (time to relapse: 24.4 months). Rituximab was well tolerated although 65 adverse events, mostly infections, were recorded within one year. Conclusion Rituximab is potentially efficacious for the treatment of patients with refractory SLE.


Asunto(s)
Inmunosupresores/uso terapéutico , Lupus Eritematoso Sistémico/tratamiento farmacológico , Nefritis Lúpica/tratamiento farmacológico , Vasculitis por Lupus del Sistema Nervioso Central/tratamiento farmacológico , Rituximab/uso terapéutico , Adolescente , Adulto , Anciano , Distribución de Chi-Cuadrado , Femenino , Humanos , Inmunosupresores/efectos adversos , Japón , Lupus Eritematoso Sistémico/diagnóstico , Lupus Eritematoso Sistémico/inmunología , Nefritis Lúpica/diagnóstico , Nefritis Lúpica/inmunología , Vasculitis por Lupus del Sistema Nervioso Central/diagnóstico , Vasculitis por Lupus del Sistema Nervioso Central/inmunología , Masculino , Persona de Mediana Edad , Análisis Multivariante , Recurrencia , Inducción de Remisión , Estudios Retrospectivos , Factores de Riesgo , Rituximab/efectos adversos , Factores de Tiempo , Resultado del Tratamiento , Adulto Joven
7.
Mucosal Immunol ; 11(3): 796-810, 2018 05.
Artículo en Inglés | MEDLINE | ID: mdl-29067999

RESUMEN

Sensitization to inhaled allergens is dependent on activation of conventional dendritic cells (cDCs) and on the adaptor molecule, MyD88. However, many cell types in the lung express Myd88, and it is unclear how signaling in these different cell types reprograms cDCs and leads to allergic inflammation of the airway. By combining ATAC-seq with RNA profiling, we found that MyD88 signaling in cDCs maintained open chromatin at select loci even at steady state, allowing genes to be rapidly induced during allergic sensitization. A distinct set of genes related to metabolism was indirectly controlled in cDCs through MyD88 signaling in airway epithelial cells (ECs). In mouse models of asthma, Myd88 expression in ECs was critical for eosinophilic inflammation, whereas Myd88 expression in cDCs was required for Th17 cell differentiation and consequent airway neutrophilia. Thus, both cell-intrinsic and cell-extrinsic MyD88 signaling controls gene expression in cDCs and orchestrates immune responses to inhaled allergens.


Asunto(s)
Asma/inmunología , Células Dendríticas/inmunología , Eosinófilos/inmunología , Hipersensibilidad/inmunología , Factor 88 de Diferenciación Mieloide/metabolismo , Mucosa Respiratoria/fisiología , Células Th17/inmunología , Administración por Inhalación , Alérgenos/inmunología , Animales , Comunicación Celular , Diferenciación Celular , Modelos Animales de Enfermedad , Regulación de la Expresión Génica , Humanos , Inmunización , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Factor 88 de Diferenciación Mieloide/genética , Transducción de Señal
8.
Phys Chem Chem Phys ; 20(5): 3286-3295, 2018 Jan 31.
Artículo en Inglés | MEDLINE | ID: mdl-29143836

RESUMEN

A series of [7]helicene and [7]helicene-like compounds composed of a cyclopenta[1,2-b:4,3-b']dithiophene or dithieno[2,3-b:3',2'-d]heterole moiety and two naphthalene moieties were successfully synthesized from a common synthetic intermediate, 1,1'-binaphtho[2,1-b]thiophene. Their helical structures were confirmed by X-ray crystallographic analysis. The photophysical properties of them and their benzene analogues were investigated via absorption and fluorescence spectroscopy and theoretical calculations to correlate the effect of the five-membered rings in their π-conjugated skeleton. Through these investigations, the photophysical properties were found to largely depend on a combination of the central five-membered ring and the neighboring two aromatic rings. In particular, a combination of the central five-membered ring with electron-withdrawing character and the two neighboring thiophene rings was revealed to induce red-shifted emission.

9.
Phys Rev Lett ; 119(22): 222501, 2017 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-29286806

RESUMEN

Fission-fragment mass distributions were measured for ^{237-240}U, ^{239-242}Np, and ^{241-244}Pu populated in the excitation-energy range from 10 to 60 MeV by multinucleon transfer channels in the reaction ^{18}O+^{238}U at the Japan Atomic Energy Agency tandem facility. Among them, the data for ^{240}U and ^{240,241,242}Np were observed for the first time. It was found that the mass distributions for all the studied nuclides maintain a double-humped shape up to the highest measured energy in contrast to expectations of predominantly symmetric fission due to the washing out of nuclear shell effects. From a comparison with the dynamical calculation based on the fluctuation-dissipation model, this behavior of the mass distributions was unambiguously attributed to the effect of multichance fission.

10.
Transplant Proc ; 49(1): 73-77, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28104163

RESUMEN

BACKGROUND: Hyperuricemia is a common adverse event frequently found in renal transplant recipients with mizoribine (MZ). Hyperuricemia itself will be a cause of renal dysfunction, and renal dysfunction also will be a cause of hyperuricemia simultaneously. This study investigates frequency of hyperuricemia and renal failure in renal transplant recipients treated with high-dose MZ. PATIENTS AND METHODS: From December 2007 to October 2015, there was a total of 32 living related renal transplant recipients treated with high-dose MZ. Of the 32 patients, 28 were treated with urate-lowering medications. RESULTS: One patient received allopurinol (AP) and 13 patients received benzbromarone (BB). For 6 of them, their urate-lowering medications were converted to febuxostat (FX) form AP or BB. In the remaining 14 patients, FX was administered from the beginning. In 2 cases of ABO-incompatible living related renal transplant recipients who were maintained with high-dose MZ and BB, severe hyperuricemia and acute renal failure occurred. One patient was a 48-year-old man, and his creatinine (Cr) level increased to 8.14 mg/dL and his serum uric acid (UA) was 24.6 mg/dL. Another patient was a 57-year-old man, and his Cr level increased to 3.59 mg/dL and his UA was 13.2 mg/dL. In both cases Cr and UA were improved, and no finding of acute rejection and drug toxicity was observed in graft biopsy specimens. BB was switched to FX and discontinuance or reduction of MZ was done. CONCLUSION: Combination of MZ and BB has the risk of acute renal dysfunction after renal transplantation. Latent renal dysfunction should be watched for in renal transplant recipients receiving high-dose MZ.


Asunto(s)
Lesión Renal Aguda/epidemiología , Lesión Renal Aguda/etiología , Hiperuricemia/epidemiología , Hiperuricemia/etiología , Trasplante de Riñón/efectos adversos , Adulto , Alopurinol/uso terapéutico , Benzbromarona/efectos adversos , Febuxostat/uso terapéutico , Humanos , Inmunosupresores/efectos adversos , Masculino , Persona de Mediana Edad , Ribonucleósidos/efectos adversos , Ribonucleósidos/uso terapéutico , Receptores de Trasplantes , Ácido Úrico/sangre , Uricosúricos/efectos adversos
11.
J Periodontal Res ; 52(3): 471-478, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-27561677

RESUMEN

BACKGROUND AND OBJECTIVE: Hypophosphatasia is a rare inherited skeletal disorder characterized by defective bone mineralization and deficiency of tissue non-specific alkaline phosphatase (TNSALP) activity. The disease is caused by mutations in the liver/bone/kidney alkaline phosphatase gene (ALPL) encoding TNSALP. Early exfoliation of primary teeth owing to disturbed cementum formation, periodontal ligament weakness and alveolar bone resorption are major complications encountered in oral findings, and discovery of early loss of primary teeth in a dental examination often leads to early diagnosis of hypophosphatasia. Although there are no known fundamental treatments or effective dental approaches to prevent early exfoliation of primary teeth in affected patients, several possible treatments have recently been described, including gene therapy. Gene therapy has also been applied to TNSALP knockout mice (Alpl-/- ), which phenocopy the infantile form of hypophosphatasia, and improved their systemic condition. In the present study, we investigated whether gene therapy improved the dental condition of Alpl-/- mice. MATERIAL AND METHODS: Following sublethal irradiation (4 Gy) at the age of 2 d, Alpl-/- mice underwent gene therapy using bone marrow cells transduced with a lentiviral vector expressing a bone-targeted form of TNSALP injected into the jugular vein (n = 3). Wild-type (Alpl+/+ ), heterozygous mice (Alpl+/- ) and Alpl-/- mice were analyzed at 9 d of age (n = 3 of each), while Alpl+/+ mice and treated or untreated Alpl-/- mice were analyzed at 1 mo of age (n = 3 of each), and Alpl+/- mice and Alpl-/- mice with gene therapy were analyzed at 3 mo of age (n = 3 of each). A single mandibular hemi-section obtained at 1 mo of age was analyzed using a small animal computed tomography machine to assess alveolar bone formation. Other mandibular hemi-sections obtained at 9 d, 1 mo and 3 mo of age were subjected to hematoxylin and eosin staining and immunohistochemical analysis of osteopontin, a marker of cementum. RESULTS: Immunohistochemical analysis of osteopontin, a marker of acellular cementum, revealed that Alpl-/- mice displayed impaired formation of cementum and alveolar bone, similar to the human dental phenotype. Cementum formation was clearly present in Alpl-/- mice that underwent gene therapy, but did not recover to the same level as that in wild-type (Alpl+/+ ) mice. Micro-computed tomography examination showed that gene therapy improved alveolar bone mineral density in Alpl-/- mice to a similar level to that in Alpl+/+ mice. CONCLUSIONS: Our results suggest that gene therapy can improve the general condition of Alpl-/- mice, and induce significant alveolar bone formation and moderate improvement of cementum formation, which may contribute to inhibition of early spontaneous tooth exfoliation.


Asunto(s)
Terapia Genética/métodos , Hipofosfatemia/terapia , Exfoliación Dental/etiología , Fosfatasa Alcalina/genética , Proceso Alveolar/patología , Animales , Densidad Ósea , Cemento Dental/patología , Modelos Animales de Enfermedad , Hipofosfatemia/complicaciones , Ratones , Ratones Noqueados , Exfoliación Dental/terapia , Resultado del Tratamiento
12.
Horm Metab Res ; 48(8): 540-9, 2016 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-27341475

RESUMEN

The present study aimed at establishing a new cryopreservation method for mouse pancreatic islets by vitrification using hollow fibers as a container. A unique feature of the hollow fiber vitrification (HFV) method is that this method achieves stable vitrification using a minimum volume of cryoprotectant (CPA) solution, thereby ensuring high viability of the islets. The cytotoxicity, optimum composition, and concentration of the CPAs for vitrifying islets were examined. The viability, functional-integrity of vitrified islets were evaluated in comparison with those vitrified by conventional methods. Insulin secretion was measured in vitro by a static incubation assay and the metabolic functions was tested after transplantation into Streptozotocin-induced diabetic mice. The combination of 15% dimethyl sulfoxide+15% ethylene glycol resulted in the best CPA solution for the HFV of islets. HFV showed the highest viability in comparison to 2 vitrification methods, open pulled straws and vitrification with EDT324 solution. The vitrified islets stably expressed ß-cells markers NeuroD, Pancreatic and duodenal homeobox-1, and MafA. Transplantation of the vitrified islets achieved euglycemia of the host diabetic mice and response to an intraperitoneal glucose tolerance test to a similar extent as non-vitrified transplanted islets. The HFV method allows for efficient long-term cryopreservation of islets.


Asunto(s)
Criopreservación/métodos , Islotes Pancreáticos/fisiología , Vitrificación , Animales , Crioprotectores/farmacología , Técnica del Anticuerpo Fluorescente , Islotes Pancreáticos/efectos de los fármacos , Trasplante de Islotes Pancreáticos , Masculino , Ratones Endogámicos ICR , Ratones SCID , Concentración Osmolar , Soluciones , Temperatura , Supervivencia Tisular/efectos de los fármacos , Vitrificación/efectos de los fármacos
15.
J Appl Microbiol ; 119(4): 1148-57, 2015 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-26176557

RESUMEN

AIMS: Streptococcus mutans produces multiple glucan-binding proteins (Gbps), among which GbpC encoded by the gbpC gene is known to be a cell-surface-associated protein involved in dextran-induced aggregation. The purpose of the present study was to characterize the dextran-binding domain of GbpC using bioinformatics analysis and molecular techniques. METHODS AND RESULTS: Bioinformatics analysis specified five possible regions containing molecular binding sites termed GB1 through GB5. Next, truncated recombinant GbpC (rGbpC) encoding each region was produced using a protein expression vector and five deletion mutant strains were generated, termed CDGB1 through CDGB5 respectively. The dextran-binding rates of truncated rGbpC that included the GB1, GB3, GB4 and GB5 regions in the upstream sequences were higher than that of the construct containing GB2 in the downstream region. In addition, the rates of dextran-binding for strains CDGB4 and CD1, which was entire gbpC deletion mutant, were significantly lower than for the other strains, while those of all other deletion mutants were quite similar to that of the parental strain MT8148. Biofilm structures formed by CDGB4 and CD1 were not as pronounced as that of MT8148, while those formed by other strains had greater density as compared to that of CD1. CONCLUSION: Our results suggest that the dextran-binding domain may be located in the GB4 region in the interior of the gbpC gene. SIGNIFICANCE AND IMPACT OF THE STUDY: Bioinformatics analysis is useful for determination of functional domains in many bacterial species.


Asunto(s)
Proteínas Bacterianas/metabolismo , Proteínas Portadoras/metabolismo , Dextranos/metabolismo , Lectinas/metabolismo , Streptococcus mutans/metabolismo , Secuencia de Aminoácidos , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Proteínas Portadoras/química , Proteínas Portadoras/genética , Lectinas/química , Lectinas/genética , Datos de Secuencia Molecular , Estructura Terciaria de Proteína , Streptococcus mutans/química , Streptococcus mutans/genética
16.
Oral Dis ; 21(7): 886-93, 2015 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-26205098

RESUMEN

OBJECTIVE: Cerebral hemorrhage has been shown to occur in animals experimentally infected with Streptococcus mutans carrying the collagen-binding Cnm gene. However, the relationship between cerebral microbleeds and oral hygiene, with a focus on Cnm gene-positive S. mutans infection, remains unclear. MATERIAL AND METHODS: One hundred and thirty-nine subjects participated. The presence or absence of Cnm-positive S. mutans and its collagen-binding activity were investigated using saliva samples, and relationship with cerebral microbleeds detected on MRI investigated, including clinical information and oral parameters. RESULTS: Fifty-one subjects were identified as Cnm-positive S. mutans carriers (36.7%), with cerebral microbleeds being detected in 43 (30.9%). A significantly larger number of subjects carried Cnm-positive S. mutans in the cerebral microbleeds (+) group. S. mutans with Cnm collagen-binding ability was detected in 39 (28.1%) of all subjects, and the adjusted odds ratio for cerebral microbleeds in the Cnm-positive group was 14.4. Regarding the presence of cerebral microbleeds, no significant differences were noted in the number of remaining teeth, dental caries, or in classic arteriosclerosis risk factors. CONCLUSIONS: The occurrence of cerebral microbleeds was higher in subjects carrying Cnm-positive S. mutans, indicating that the presence of Cnm-positive S. mutans increases cerebral microbleeds, and is an independent risk for the development of cerebrovascular disorders.


Asunto(s)
Adhesinas Bacterianas/genética , Proteínas Portadoras/genética , Portador Sano/microbiología , Hemorragia Cerebral/epidemiología , Saliva/microbiología , Infecciones Estreptocócicas/microbiología , Streptococcus mutans/genética , Anciano , Portador Sano/diagnóstico , Hemorragia Cerebral/diagnóstico por imagen , Colágeno/metabolismo , Femenino , Humanos , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Higiene Bucal , Saliva/metabolismo , Infecciones Estreptocócicas/diagnóstico , Streptococcus mutans/metabolismo
17.
Mucosal Immunol ; 8(1): 186-97, 2015 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-24985082

RESUMEN

Allergic asthma is thought to stem largely from maladaptive T helper 2 (Th2) responses to inhaled allergens, which in turn lead to airway eosinophilia and airway hyperresponsiveness (AHR). However, many individuals with asthma have airway inflammation that is predominantly neutrophilic and resistant to treatment with inhaled glucocorticoids. An improved understanding of the molecular basis of this form of asthma might lead to improved strategies for its treatment. Here, we identify novel roles of the adaptor protein, TRIF (TIR-domain-containing adapter-inducing interferon-ß), in neutrophilic responses to inhaled allergens. In different mouse models of asthma, Trif-deficient animals had marked reductions in interleukin (IL)-17, airway neutrophils, and AHR compared with wild-type (WT) mice, whereas airway eosinophils were generally similar in these two strains. Compared with lung dendritic cells (DCs) from WT mice, lung DCs from Trif-deficient mice displayed impaired lipopolysaccharide (LPS)-induced migration to regional lymph nodes, lower levels of the costimulatory molecule, CD40, and produced smaller amounts of the T helper 17 (Th17)-promoting cytokines, IL-6, and IL-1ß. When cultured with allergen-specific, naive T cells, Trif-deficient lung DCs stimulated robust Th2 cell differentiation but very weak Th1 and Th17 cell differentiation. Together, these findings reveal a TRIF-CD40-Th17 axis in the development of IL-17-associated neutrophilic asthma.


Asunto(s)
Proteínas Adaptadoras del Transporte Vesicular/metabolismo , Asma/inmunología , Hiperreactividad Bronquial/inmunología , Células Dendríticas/fisiología , Eosinófilos/fisiología , Neutrófilos/fisiología , Subgrupos de Linfocitos T/inmunología , Células Th17/inmunología , Proteínas Adaptadoras del Transporte Vesicular/genética , Animales , Antígenos Dermatofagoides/inmunología , Antígenos CD40/metabolismo , Movimiento Celular/genética , Células Cultivadas , Citocinas/metabolismo , Modelos Animales de Enfermedad , Humanos , Pulmón/inmunología , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Material Particulado/inmunología , Balance Th1 - Th2
18.
Oral Dis ; 20(7): 700-6, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-25360469

RESUMEN

OBJECTIVE: Streptococcus mutans, a major dental caries pathogen, has shown to be associated with the aggravation of cerebral hemorrhage and inflammatory bowel diseases. In this study, we evaluated the effects ofS. mutans on the development of non-alcoholic steatohepatitis (NASH) in a mouse model. MATERIALS AND METHODS: Streptococcus mutans oral strain MT8148 (serotype c) and a blood isolate TW871 (k) were used. C57BL/6J mice (6 weeks old)were fed a high-fat diet for 4 weeks; the test strains or phosphate-buffered saline was then intravenously administered. Mice were euthanized after 8 or 12 weeks. Whole body, extirpated liver, and visceral fat weights were determined, and histopathological evaluations of the liver specimens were performed. RESULTS: Mice infected with TW871 showed significantly greater body and liver weights than those administered MT8148 or phosphate-buffered saline. Histopathological analyses revealed prominent infiltration of inflammatory cells and adipocellular deposition in livers extirpated 8 weeks after an infection with TW871; fibrosis was also observed in livers extirpated after 12 weeks. CONCLUSION: These results suggest that a specific strain of S. mutans could induce NASH.


Asunto(s)
Enfermedad del Hígado Graso no Alcohólico/microbiología , Infecciones Estreptocócicas/complicaciones , Streptococcus mutans , Animales , Modelos Animales de Enfermedad , Masculino , Ratones , Ratones Endogámicos C57BL , Índice de Severidad de la Enfermedad
19.
Transplant Proc ; 46(2): 626-9, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24656030

RESUMEN

BACKGROUND: Renal cancers commonly occur in the native kidneys of renal transplant recipients, whereas renal cancer in the grafted kidney has been reported occasionally. Renal cancer in the grafted kidney occurred 16 years after graft loss in this case, which would be a more rare case. CASE REPORT: A 60-year-old man who had a kidney transplant from his mother at the age of 31 years and had hemodialysis again because of chronic rejection from the age of 44 years had right lower abdominal pain. Computerized tomography (CT) showed tumor involvement in the grafted kidney. Positron-emission tomography-CT also showed hot spots in the liver, cervical vertebra, and costal bone. Needle biopsy for grafted kidney and liver tumors were done, and pathologic findings revealed renal cancer of grafted kidney and metastatic liver tumor. Graftectomy was done, and renal cancer was diagnosed as spindle cell carcinoma. Irradiation for cervical bone metastasis was done after the surgery. He complained of abdominal pain and eating disturbance 2 months after the surgery. CT showed a huge recurrence tumor and multiple tumor dissemination. Small intestine was involved and obstructed by the main tumor. He died of recurrence of renal cancer 3 months after the surgery. CONCLUSIONS: It is reported that the rate of renal cell carcinoma in the grafted kidney was 0.19%-0.5% and it occurred at a mean of 12.6 years after renal transplantation. Herein, we report a rare case of renal cancer that occurred 29 years after renal transplantation. Long-term observation should be required for recipients who had rehemodialysis.


Asunto(s)
Rechazo de Injerto , Trasplante de Riñón , Resultado Fatal , Humanos , Neoplasias Renales/diagnóstico por imagen , Neoplasias Renales/patología , Masculino , Persona de Mediana Edad , Metástasis de la Neoplasia , Tomografía de Emisión de Positrones , Tomografía Computarizada por Rayos X
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