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J Matern Fetal Neonatal Med ; 29(21): 3496-505, 2016 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-26742485


OBJECTIVE: Tocolytic drugs are used widely in order to prevent preterm birth. Ritodrine, is the only food and drug administration (FDA) approved drug for tocolytic use. We estimated the cytogenetic effect of ritodrine administered as maternal therapy, alone or in combination with smoking, in women and their neonates. METHODS: Lymphocyte and fibroblasts cultures were evaluated and three indices were analyzed; sister chromatid exchanges (SCEs), proliferation rate index (PRI) and mitotic index (MI) as well as average generation time (AGT) and population doubling time (PDT). Campothacin (CPT-11) was used as a positive control. RESULTS: Administration of ritodrine up to a month revealed significant reduction of SCEs/cell in neonates in the presence or absence of the mutagenic agent. A statistical significant increase on SCEs, for mothers and neonates, was noticed in neonate's lymphocytes when tocolytic therapy was over a month. Ritodrine revealed a cytoprotective action against smoking when the two factors were combined, but the synergistic action of ritodrine with smoking increased genotoxicity, cytostaticity and cytotoxicity of neonates after long administration (1-3 months). CONCLUSIONS: The time-depended genotoxic, cytostatic and cytotoxic action of ritodrine alone or in combination with smoking suggests that its administration should not exceed the time period of a month.

Fibroblastos/efectos de los fármacos , Linfocitos/efectos de los fármacos , Trabajo de Parto Prematuro/tratamiento farmacológico , Nacimiento Prematuro/tratamiento farmacológico , Ritodrina/efectos adversos , Fumar/efectos adversos , Tocolíticos/efectos adversos , Adulto , Análisis de Varianza , Estudios de Casos y Controles , Proliferación Celular , Femenino , Edad Gestacional , Humanos , Recién Nacido , Masculino , Índice Mitótico , Embarazo , Nacimiento Prematuro/prevención & control , Ritodrina/administración & dosificación , Intercambio de Cromátides Hermanas , Factores de Tiempo , Tocolíticos/administración & dosificación
J Matern Fetal Neonatal Med ; 27(2): 127-31, 2014 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-23682763


OBJECTIVE: The examination of the genotoxic, cytostatic and cytotoxic effects of smoking during pregnancy. METHOD: Lymphocyte cultures of peripheral blood were received from 20 women who smoked during pregnancy as well as umbilical cord blood of their newborns. Fluorescence Plus Giemsa staining technique was used in order to perform cytogenetic analyses for three indices, Sister Chromatid Exchanges (SCEs), Proliferation Rate Index (PRI) and Mitotic Index (MI). To reveal any underlying chromosome instability, CPT-11 was used as a positive control. RESULTS: Newborns whose mothers smoke during pregnancy had increased SCEs levels on their lymphocytes when they were exposed to the mutagenic agent CPT-11 (p < 0.01) compared with newborns lymphocytes exposed to the same agent with non-smoking mothers. Also, mothers smoking during pregnancy had increased SCE levels when their lymphocytes were exposed to CPT-11 (p < 0.01) compared with non smoking mothers whose lymphocytes were exposed to the same agent. In both groups newborns appeared as having decreased (p < 0.01) spontaneous SCEs levels compared with the corresponding SCE rates of their mothers. Decreases of PRIs and MIs are observed in mothers compared to their newborns. CONCLUSION: Smoking during pregnancy can promote cytogenetic damage in newborn's DNA, causing chromosome instability. The clinical importance of this indirect damage lies in the fact that this type of damage can act synergistically with other environmental and/or chemical mutagenic substances possibly leading to carcinogenicity.

Análisis Citogenético , Intercambio Materno-Fetal , Fumar/efectos adversos , Adolescente , Adulto , Camptotecina/análogos & derivados , Camptotecina/farmacología , Proliferación Celular , Células Cultivadas , Daño del ADN/efectos de los fármacos , Daño del ADN/genética , Femenino , Sangre Fetal/citología , Humanos , Recién Nacido , Irinotecán , Linfocitos , Índice Mitótico , Mutágenos/administración & dosificación , Embarazo , Intercambio de Cromátides Hermanas , Fumar/sangre , Adulto Joven
Eur J Obstet Gynecol Reprod Biol ; 165(2): 205-9, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-22921575


OBJECTIVE: To study cytogenetic damage in order to estimate the effect of pre-pregnancy smoking on pregnant women and their foetuses. STUDY DESIGN: Lymphocyte cultures were obtained from peripheral blood of 20 women who quit smoking during pregnancy, and umbilical cord blood of their newborns at delivery. Cytogenetic analyses were performed for sister chromatid exchanges (SCEs), proliferation rate index (PRI) and mitotic index (MI) using the Fluorescence Plus Giemsa staining technique. Twenty non-smoking women and their newborns were evaluated as controls. CPT-11, a known antineoplastic, was used as a positive genotoxic agent in order to correlate non-smoking women with smoking women and reveal any underlying chromosome instability. Statistical evaluation of SCE frequencies, PRI and MI was based on independent samples t-test in order to estimate the effect of pre-pregnancy smoking on mothers and their newborns. RESULTS: SCEs were induced in the cord blood lymphocytes of newborns whose mothers smoked before pregnancy when they were exposed to the mutagenic agent CPT-11 (p<0.01). A similar increase in SCEs was observed in both non-smoking and smoking mothers exposed to CPT-11. Newborns in both groups had significantly lower SCE levels than their mothers (p<0.01). CONCLUSION: Pre-pregnancy smoking results in cytogenetic damage for both mothers and newborns, and is an important risk factor for cancer and/or other genetic-related diseases. Smoking cessation needs to occur well before conception in order to avoid the strong cytogenetic association between pre-pregnancy smoking by mothers and their newborns.

Sangre Fetal/citología , Linfocitos/citología , Exposición Materna , Fumar/sangre , Adulto , Citogenética , Femenino , Humanos , Recién Nacido , Embarazo , Intercambio de Cromátides Hermanas , Cese del Hábito de Fumar
Minim Invasive Ther Allied Technol ; 20(3): 155-9, 2011 May.
Artículo en Inglés | MEDLINE | ID: mdl-21082900


We performed an evaluation of ultrasound-guided transvaginal aspiration of ovarian cysts as a viable alternative to surgery in 104 reproductive and 17 postmenopausal women. One-hundred and twenty-one patients with a simple >4 cm diameter ovarian cyst, with a benign appearance on ultrasound as well as on clinical and blood examination, underwent transvaginal fine needle aspiration of the cyst under ultrasonographic control. One-hundred and four patients were of reproductive age and 12 were postmenopausal. Sixty women who were of reproductive age and in which OCP treatment was not contraindicated followed a six-month therapy with oral contraceptives after the intervention. In the group of patients of reproductive age under OCP treatment the cyst persisted in nine of the 60 women (recurrence rate 15%). In the other group of patients of reproductive age,under no OCP treatment, the recurrence rate was 47% (21 of the 44 women). In the group of postmenopausal patients, the cyst persisted in ten out of 17 cases (recurrence rate 58,9%). Transvaginal aspiration of ovarian cysts is a reliable alternative to surgery with many advantages such as excellent tolerance, low risk and cost of complications and recurrence. The OCP treatment after aspiration seems to increase the success rate of expectant management.

Biopsia con Aguja/métodos , Anticonceptivos Orales/uso terapéutico , Quistes Ováricos/terapia , Adolescente , Adulto , Factores de Edad , Anciano , Biopsia con Aguja/efectos adversos , Terapia Combinada , Femenino , Estudios de Seguimiento , Humanos , Persona de Mediana Edad , Quistes Ováricos/diagnóstico por imagen , Quistes Ováricos/patología , Recurrencia , Estudios Retrospectivos , Resultado del Tratamiento , Ultrasonografía Intervencional/métodos , Vagina , Adulto Joven