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1.
Phys Rev Lett ; 124(6): 067701, 2020 Feb 14.
Artículo en Inglés | MEDLINE | ID: mdl-32109120

RESUMEN

Spins in silicon quantum devices are promising candidates for large-scale quantum computing. Gate-based sensing of spin qubits offers a compact and scalable readout with high fidelity, however, further improvements in sensitivity are required to meet the fidelity thresholds and measurement timescales needed for the implementation of fast feedback in error correction protocols. Here, we combine radio-frequency gate-based sensing at 622 MHz with a Josephson parametric amplifier, that operates in the 500-800 MHz band, to reduce the integration time required to read the state of a silicon double quantum dot formed in a nanowire transistor. Based on our achieved signal-to-noise ratio, we estimate that singlet-triplet single-shot readout with an average fidelity of 99.7% could be performed in 1 µs, well below the requirements for fault-tolerant readout and 30 times faster than without the Josephson parametric amplifier. Additionally, the Josephson parametric amplifier allows operation at a lower radio-frequency power while maintaining identical signal-to-noise ratio. We determine a noise temperature of 200 mK with a contribution from the Josephson parametric amplifier (25%), cryogenic amplifier (25%) and the resonator (50%), showing routes to further increase the readout speed.

3.
Genet Mol Res ; 15(3)2016 Sep 23.
Artículo en Inglés | MEDLINE | ID: mdl-27706786

RESUMEN

Nontuberculous mycobacteria are ubiquitous in outside environment and animals. As for nontuberculous mycobacteria infection, there is only limited information in humans regarding infection and the subsequent immune response, especially for Mycobacterium neoaurum. Here, haematoxylin-eosin and Ziehl-Neelsen staining were used to observe pathological changes and detect acid-fast bacilli in organ samples in mouse model. Flow cytometry and quantitative real-time polymerase chain reaction were performed to analyze the contribution of Th1, Th17 and Tregs to the host immune response. M. neoaurum caused chronic infection in mice, resulting in infiltrates with large aggregates of inflammatory cells, especially macrophages, in lung tissues. Our results indicated that 72% of CD4+ T cells appeared in the early days of infection, which was followed by a decrease to 47% by day 32, and then a rise to 76% by day 56. Moreover, we found higher frequency of IFN-g-producing CD4+ T cells and elevated mRNA expression of the transcription factor T-bet in the lungs; however, we observed lower mRNA expression of the transcription factor RORgt and lower frequency of IL-17-producing CD4+ T cells. A transient relative decrease in the number of Treg cells was observed in the lungs; however, the number of Tregs did not change significantly between the first and last day following infection. Thus, M. neoaurum causes chronic infection in C57BL/6 mice, with Th1, Th17, and Tregs playing a prominent role in the host response. The present study may lay the basis for further studies on the mechanisms underlying infection with nontuberculous mycobacteria.


Asunto(s)
Interacciones Huésped-Patógeno/inmunología , Infecciones por Mycobacterium/inmunología , Infecciones por Mycobacterium/microbiología , Mycobacterium/inmunología , Linfocitos T Reguladores/inmunología , Células TH1/inmunología , Células Th17/inmunología , Inmunidad Adaptativa , Animales , Carga Bacteriana/inmunología , Recuento de Colonia Microbiana , Femenino , Factores de Transcripción Forkhead/metabolismo , Regulación de la Expresión Génica , Pulmón/metabolismo , Pulmón/microbiología , Pulmón/patología , Subgrupos Linfocitarios/inmunología , Ratones Endogámicos C57BL , Mycobacterium/crecimiento & desarrollo , Infecciones por Mycobacterium/patología , ARN Mensajero/genética , ARN Mensajero/metabolismo
4.
Poult Sci ; 95(12): 2889-2894, 2016 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-27287385

RESUMEN

The incorporation of carbonate has been recognized as an evident change in bone mineral (bioapatite) during aging. Laying hens (Gushi layer) at 4 stages of age (8 hens each stage) were studied by Raman spectroscopy and X-ray radiography to investigate the mineralogical changes and bone density, respectively. Cortical bones of the humerus and femur show a rapid increase of carbonate (∼1.9 wt.%) from sexual maturity to the peak of hens' laying period, while the densities of the cortical bones are relatively stable. Before sexual maturity, the density of the cortical bones increases considerably during aging. However, after the peak of the laying period, only femoral density continues elevating. Carbonate contents in the cortical bones reach the maximum at the peak of the laying period. Two pathways (halted growth of density and Ca-release due to the CO3 incorporation) could both contribute to the intense Ca requirement for egg laying. Crystallization, however, has no significant changes during aging and the laying period. This study could shed light on the mechanism of mineral losses due to CO3 incorporation, and also shows the advantages of Raman spectroscopy in tracking mineral loss in poultry bone.


Asunto(s)
Carbonatos/análisis , Pollos/metabolismo , Fémur/química , Húmero/química , Oviposición/fisiología , Animales , Densidad Ósea , Calcificación Fisiológica/fisiología , Pollos/fisiología , Femenino , Fémur/diagnóstico por imagen , Fémur/metabolismo , Fémur/fisiología , Húmero/diagnóstico por imagen , Húmero/metabolismo , Húmero/fisiología , Radiografía , Espectrometría Raman
5.
Zhonghua Xin Xue Guan Bing Za Zhi ; 44(12): 1036-1039, 2016 Dec 24.
Artículo en Chino | MEDLINE | ID: mdl-28056235

RESUMEN

Objective: Right heart catheterization is the gold-standard for measurement of right ventricular hemodynamics. However, it is difficult to perform in mice. We thus aimed to establish a new open chest method to record and analyze the right ventricular hemodynamic parameters in intubated mice connected to a respirator under anesthesia. Methods: Eight-week old male C57BL/6 mice were divided into the control group, and the hypoxia group using completely random method. Mice in the hypoxia group were exposed in low pressure oxygen chamber, which simulated the environment of plateau (5 500 m altitude, 10% oxygen) for 3 weeks. Mice in the control group were exposed in normal pressure and oxygen environment. The mice were intratracheally intubated and connected to a respirator, chest was opened, right ventricular hemodynamics were measured with a needle (0.7 mm×19 mm) punctured into right ventricle (5 mm in death), which was connected to the measuring equipment. Results: The open chest procedure could be finished within 5-10 minutes. The successful rate was 100%. There was no significant difference in heart rate between control group and hypoxia group under anesthetia ((306.4±11.5) bpm vs.(320.4±16.0) bpm, P>0.05). Compared with the control group, both the right ventricular systolic pressure (RVSP) and the mean right ventricular pressure(mRVP)were increased in hypoxia group ((17.1±1.0)mmHg(1 mmHg=0.133 kPa) vs. (22.6±1.0) mmHg, P<0.01; (9.6±0.8) mmHg vs.(12.4±0.3) mmHg, P<0.01). Moreover, the absolute value of the maximal rate of increase in right ventricular pressure (dp/dtmax) and the maximal rate of decrease in right ventricular pressure (dp/dtmin) were higher in the hypoxia group ((421.3±30.6)mmHg/s vs.(639.7±47.7)mmHg/s, P<0.01; (-324.3±24.0)mmHg/s vs. (-496.5±40.6) mmHg/s, P<0.01), indicating the pathological status after hypoxia treatment. Conclusions: Right ventricular hemodynamics measurement with this open chest technique is easy to handle and can accurately reflect the right ventricular pressure in mice under physiological and pathological conditions.


Asunto(s)
Ventrículos Cardíacos , Animales , Cateterismo Cardíaco , Frecuencia Cardíaca , Hemodinámica , Hipertensión Pulmonar , Masculino , Ratones , Ratones Endogámicos C57BL
6.
Zhongguo Zhong Yao Za Zhi ; 26(12): 831-4, 2001 Dec.
Artículo en Chino | MEDLINE | ID: mdl-12776329

RESUMEN

OBJECTIVE: To study the pharmaceutic chemical basis of the different medicinal effects, and to compare and analyze the sterols in Hericium erinaceus mycelia and its ethanol extract, water extract derived from solid fermented mycelia. METHOD: The components of Hericium erinaceus mycelia and two kinds of extract were compared with some biochemical methods such as GC, RP-HPLC, etc. Sterol composition in Hericium erinaceus mycelia was determined by using GC-mass spectrometry. RESULTS: The content of crude polysaccharide, water-soluble protein and fatty acid of ethanol extract showed no obvious advantage over its water extract. However, there was significant difference between two kinds of extract in the content of sterols. And ergostane derivatives, beta-sitosterol and C28 sterol with four bonds were detected as the major sterols in Hericium erinaceus mycelia, among which ergosterol was the principal sterol. CONCLUSION: Sterols in Hericium erinaceus exist mainly in ethanol extract. And three sterols are found for the first time from Hericium erinaceus, which are ergostane derivatives, beta-sitosterol and C28 sterol with four bonds.


Asunto(s)
Basidiomycota/química , Ergosterol/análisis , Etanol , Micelio/química , Agua
7.
Zhongguo Zhong Yao Za Zhi ; 25(12): 710-3, 2000 Dec.
Artículo en Chino | MEDLINE | ID: mdl-12525056

RESUMEN

OBJECTIVE: To evaluate the wind-damage effects on quality of heartwood of Lignum Santali Albi. METHOD: GC-MS, TLC and pharmacodynamic test. RESULTS: The content of volatile oil from heartwood of Wind-damaged Lignum Santali Albi is 1.42%; the content of various components in the oil and the chromatography of different extracts are similar to those of reference drug and 25 years old trees. CONCLUSION: Wind-damage should accelerate the formation of heartwood of Lignum Santali Albi without influence on its quality.


Asunto(s)
Medicamentos Herbarios Chinos/análisis , Aceites Volátiles/análisis , Plantas Medicinales/química , Santalaceae/química , Animales , Medicamentos Herbarios Chinos/farmacología , Femenino , Íleon/efectos de los fármacos , Masculino , Ratones , Contracción Muscular/efectos de los fármacos , Músculo Liso/efectos de los fármacos , Aceites Volátiles/farmacología , Control de Calidad , Conejos , Viento
8.
Br J Haematol ; 83(3): 399-403, 1993 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-8485045

RESUMEN

The objective of this study was to investigate the prognostic impact of the reduction of Philadelphia chromosome (Ph) positive metaphases by treatment of chronic myelogenous leukaemia (CML) with interferon (IFN) alpha. Therefore, we evaluated the outcome of patients with previously untreated chronic phase Ph-positive CML, enrolled from 1984 to 1990 into two consecutive IFN trials at our institution. Of a total of 71 patients, 62 (87%) were evaluable for cytogenetic response. No cytogenetic improvement was seen in 16 patients (23%), 28 patients (38%) had a decrease in Ph-positive bone marrow metaphases to levels ranging from 35% to 95%, and nine patients (13%) to levels between 5% and 34%. In nine patients (13%), Ph-positive metaphases were no longer detectable. After a median follow-up period of 33 months, the projected 5-year survival is 55% for the 62 patients evaluable for cytogenetic response. In this patient population there was no significant difference in the survival probability according to patients' risk status as defined by the Sokal score. Categorization according to the extent of Ph reduction, however, allowed three groups with significantly different prognoses to be identified. Patients achieving a Ph reduction to less than 35% were found to constitute a low risk group with a median survival not yet known and a projected 5-year survival of 90%. The 5-year survival rate was 55% for patients with a Ph reduction to levels between 35% and 95%, and less than 10% for those without any cytogenetic improvement. Thus, this study demonstrates that cytogenetic improvement on IFN treatment is an important prognostic factor for survival.


Asunto(s)
Interferón-alfa/uso terapéutico , Leucemia Mielógena Crónica BCR-ABL Positiva/terapia , Adolescente , Adulto , Anciano , Femenino , Alemania/epidemiología , Humanos , Interferón alfa-2 , Leucemia Mielógena Crónica BCR-ABL Positiva/genética , Leucemia Mielógena Crónica BCR-ABL Positiva/mortalidad , Masculino , Persona de Mediana Edad , Probabilidad , Pronóstico , Proteínas Recombinantes , Inducción de Remisión , Factores de Riesgo , Análisis de Supervivencia
9.
Cancer Genet Cytogenet ; 57(1): 103-7, 1991 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-1756476

RESUMEN

In situ suppression hybridization with recombinant bacteriophage DNA libraries for chromosomes 8 and 21 was performed in two cases of acute nonlymphocytic leukemia, type FAB M2. In both cases, cytogenetic analysis by conventional G-banding revealed t(8;21)(q22;q22). In situ suppression hybridization was able to prove the reciprocal nature of the translocation in both cases by identifying the terminal end of chromosome 21 translocated to the derivative chromosome 8q-.


Asunto(s)
Aberraciones Cromosómicas/diagnóstico , Cromosomas Humanos Par 21/ultraestructura , Cromosomas Humanos Par 8/ultraestructura , Leucemia Mieloide Aguda/patología , Bandeo Cromosómico , Trastornos de los Cromosomas , Sondas de ADN , Humanos , Leucemia Mieloide Aguda/genética , Hibridación de Ácido Nucleico , Translocación Genética
10.
Cancer Genet Cytogenet ; 44(2): 181-6, 1990 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-2297676

RESUMEN

This paper reports on seven atypical Philadelphia chromosome translocations in chronic myelocytic leukemia. Three of them, a t(16;22), t(17;22), and t(9;14;22) have already been observed before, while the t(X;9;11;22), t(X;22), t(3;22) and t(3;4;9;22) are newly reported.


Asunto(s)
Leucemia Mielógena Crónica BCR-ABL Positiva/genética , Cromosoma Filadelfia , Translocación Genética , Adulto , Femenino , Humanos , Cariotipificación , Masculino , Persona de Mediana Edad , Cromosoma X
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