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1.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 55(11): 864-870, 2020 Nov 09.
Artículo en Chino | MEDLINE | ID: mdl-33171560

RESUMEN

Objective: To evaluate long-term clinical outcome of implant-supported fixed prosthesis with the guidance of computer-aided design/computer-aided manufacturing (CAD/CAM) surgical template in old patients. Methods: Fifteen 50-67(58.6±5.3) year-old patients (11 males and 4 females) who received the digital implant surgery with the guidance of CAD/CAM surgical template and implant-supported fixed prosthesis in Department of Oral Implantology, Yantai Stomatological Hospital Affiliated to Binzhou Medical College from August, 2013 to Janurary, 2016 were included. There are 15 edentulous maxillas, 8 edentulous mandibles on which 145 implants were placed in total. After the placement of implant, the accuracy of CAD/CAM surgical template was assessed by comparing the post-operative 3-dimensional location of implant with the pre-operative design. The 5-year survival rate of implant and prosthesis was recorded, and marginal bone loss around implants and the development of complication were evaluated by radiological and clinical examination respectively. Results: The result on accuracy of CAD/CAM surgical template showed that deviations in neck and apex of implants were (0.78±0.59) and (1.38±0.63) mm respectively, depth deviation was (0.65±0.35) mm and angle deviation was 4.12°±2.77°. The 5-year cumulative survival rate of implants was 98.6%(143/145). In all 145 implants, the prevalence of implant-related biological complication was 4.1%(6/145). The prosthesis-related mechanical complication rate was 57%(13/23). After 5-year function, mean peri-implant bone loss was (0.69±0.35) mm. Conclusions: This 5-year follow-up retrospective study showed that implant-supported fixed prosthesis with the guidance of CAD/CAM surgical template in middle aged and elderly patients could achieve good long-term clinical outcome and patient satisfaction.


Asunto(s)
Implantes Dentales , Arcada Edéntula , Cirugía Asistida por Computador , Anciano , Diseño Asistido por Computadora , Implantación Dental Endoósea , Prótesis Dental de Soporte Implantado , Femenino , Humanos , Arcada Edéntula/cirugía , Masculino , Persona de Mediana Edad , Estudios Retrospectivos
3.
Eur Rev Med Pharmacol Sci ; 24(6): 3322-3330, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-32271450

RESUMEN

OBJECTIVE: To detect differentially expressed micro ribonucleic acids (miRNAs) in rats with myocardial ischemia/reperfusion (MIR), and to explore the influence of miR-19a on MIR rats and its mechanism. MATERIALS AND METHODS: Firstly, the Sprague-Dawley (SD) rats were used to prepare MIR models, RNAs were extracted, and miRNA sequencing analysis was carried out to determine differentially expressed miRNAs related to MIR. Secondly, the predicted target genes of miR-19a were collected, and WebGestalt was applied to analyze gene ontology (GO) and pathway enrichment. Thirdly, the expression of the related proteins and the apoptosis of myocardial cells in MIR rats were detected via Western blotting. Fourthly, the interaction between miR-19a and the target gene phosphatase and tensin homolog (PTEN) was examined through Luciferase reporter assay. RESULTS: Compared with that in the Sham operation (Sham) group, the miR-19a expression in rat myocardial tissues in the MIR group was significantly increased (p<0.05). Compared with those in the miR-negative control (miR-NC) group, the messenger RNA (mRNA) and protein expressions of PTEN in the miR-19a group were notably decreased (p<0.05). In comparison with the miR-NC group, miR-19a group had elevated expression of phosphorylated protein kinase B (p-Akt) (p<0.05). The Luciferase reporter gene assay manifested the direct binding of miR-19a to PTEN mRNA. CONCLUSIONS: MiR-19a inhibits the PTEN expression by directly binding to the 3'-UTR of PTEN mRNA, thus activating the Akt/p-Akt signaling pathway to suppress the apoptosis of myocardial cells in MIR injury.

4.
Drug Des Devel Ther ; 13: 3977-3988, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-32063699

RESUMEN

Background/Aims: To investigate the postconditioning protective effect of penehyclidine hydrochloride (PHC) against anoxia/reoxygenation (A/R) injury in H9c2 cells along with the involved mechanism and timing effect. Methods: We divided H9c2 cells into 7 groups: control group, A/R group and PHC+A/R groups at 0 min, 5 mins, 10 mins, 20 mins, 30 mins, respectively (treated with 0.1 µm/L PHC at 0 min, 5 mins, 10 mins, 20 mins, 30 mins after the reoxygenation procedure began). Cell apoptosis, oxidative stress, intracellular Ca2+ concentration, mitochondrial membrane potential and mitochondrial permeability transition pore (MPTP) opening were explored. Bcl-2, Bax, Cyt C, caspase-3 and caspase-9 levels were measured. Results: A/R significantly increased both cell injury and cell apoptosis. PHC showed postconditioning protective effect by attenuating superoxide production, decreasing Ca2+ overload, restraining MPTP activities, restoring mitochondrial membrane potential, regulating cell apoptosis proteins and modulation of mitochondrial pathway. Earlier administration of PHC offered greater postconditioning protective effect. Conclusion: H9c2 cells were protected by PHC from A/R injury regardless of timing of PHC administration (0 min, 5 mins, 10 mins, 20 mins, 30 mins). However, earlier administration of PHC resulted in better PHC postconditioning protection.


Asunto(s)
Hipoxia/tratamiento farmacológico , Daño por Reperfusión Miocárdica/tratamiento farmacológico , Miocitos Cardíacos/efectos de los fármacos , Sustancias Protectoras/farmacología , Quinuclidinas/farmacología , Apoptosis/efectos de los fármacos , Línea Celular , Humanos , Hipoxia/metabolismo , Hipoxia/patología , Daño por Reperfusión Miocárdica/metabolismo , Daño por Reperfusión Miocárdica/patología , Miocitos Cardíacos/metabolismo , Miocitos Cardíacos/patología , Sustancias Protectoras/administración & dosificación , Quinuclidinas/administración & dosificación
5.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 53(10): 703-707, 2018 Oct 09.
Artículo en Chino | MEDLINE | ID: mdl-30392229

RESUMEN

FimA has been characterized as an important virulence factor for Porphyromonas gingivalis (Pg). These structures play a major role in the mechanisms of adhesion and invasion of Pg to host cells, and can induce cellular activation and cytokines release. FimA can also promote biofilm formation and induce immuno-inflammatory response of host cells. Many studies have characterized FimA to be associated with periodontitis and cardiovascular disease. Pg strains are classified into six types based on divergent nucleotide sequences of the fimA gene (types Ⅰ、Ⅰb、Ⅱ、Ⅲ、Ⅳ andⅤ). The expression of fimbriae is regulated by the fimA gene, which may be the key factor that leads to virulence diversities of Pg, At present, the research on the pathogenesis of FimA mainly focuses on periodontitis and atherosclerosis, which is of great significance for the prevention and treatment of diseases. This paper reviewed the pathogenic effect of FimA in the development of above mentioned two diseases and its application in the prevention.


Asunto(s)
Proteínas Fimbrias , Periodontitis , Porphyromonas gingivalis , Humanos , Periodontitis/microbiología , Porphyromonas gingivalis/patogenicidad , Virulencia , Factores de Virulencia
6.
Int J Oral Maxillofac Surg ; 45(12): 1665-1670, 2016 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-27481688

RESUMEN

The aim of this study was to evaluate the accuracy of novel software-CMF-preCADS-for the prediction of soft tissue changes following repositioning surgery for zygomatic fractures. Twenty patients who had sustained an isolated zygomatic fracture accompanied by facial deformity and who were treated with repositioning surgery participated in this study. Cone beam computed tomography (CBCT) scans and three-dimensional (3D) stereophotographs were acquired preoperatively and postoperatively. The 3D skeletal model from the preoperative CBCT data was matched with the postoperative one, and the fractured zygomatic fragments were segmented and aligned to the postoperative position for prediction. Then, the predicted model was matched with the postoperative 3D stereophotograph for quantification of the simulation error. The mean absolute error in the zygomatic soft tissue region between the predicted model and the real one was 1.42±1.56mm for all cases. The accuracy of the prediction (mean absolute error ≤2mm) was 87%. In the subjective assessment it was found that the majority of evaluators considered the predicted model and the postoperative model to be 'very similar'. CMF-preCADS software can provide a realistic, accurate prediction of the facial soft tissue appearance after repositioning surgery for zygomatic fractures. The reliability of this software for other types of repositioning surgery for maxillofacial fractures should be validated in the future.


Asunto(s)
Simulación por Computador , Cara , Imagenología Tridimensional , Procedimientos Quirúrgicos Reconstructivos/métodos , Fracturas Cigomáticas/cirugía , Adulto , Femenino , Humanos , Masculino , Reproducibilidad de los Resultados
7.
Beijing Da Xue Xue Bao Yi Xue Ban ; 46(6): 868-74, 2014 Dec 18.
Artículo en Chino | MEDLINE | ID: mdl-25512274

RESUMEN

OBJECTIVE: To study microRNA profile alteration in unstable angina (UA) patients and its functional network via nerve growth factor (NGF) signaling pathway. METHODS: Whole blood microRNAs were isolated from individuals with angiographically negative report (n=6) and UA patients (n=6), Taqman low-density microRNA array was performed to detect the microRNA profile, and SAM tool was conducted to calculate the differential expressed microRNA. Bioinformatic prediction tools (Targetsan, Miranda, Diana-microT) were used to obtain microRNA target genes. Targets'enriched pathways were analyzed by DAVID and biological function clusters were figured out by Panther database. The functional network of microRNAs by Cytoscape was costructed. RESULTS: In the study, 20 microRNAs were significantly upregulated in the UA group were observed. Their main target signaling pathways were NGF, their target genes' functional clusters were in signal transduction, cell proliferation and differentiation, cell cycles, immunity and inflammation, neurogenesis, neuronal activity and apoptosis. CONCLUSION: To upregulate microRNAs in UA patients is a major way to inhibit NGF signaling pathways, whose function is to suppress cell proliferation and differentiation, immunity and inflammation, neuronal growth, etc., and to stabilize the vulnerable atherosclerotic plaque.


Asunto(s)
Angina Inestable/metabolismo , MicroARNs/metabolismo , Factores de Crecimiento Nervioso/metabolismo , Transducción de Señal , Biología Computacional , Humanos
8.
Beijing Da Xue Xue Bao Yi Xue Ban ; 46(6): 894-8, 2014 Dec 18.
Artículo en Chino | MEDLINE | ID: mdl-25512279

RESUMEN

OBJECTIVE: To investigate the role of microRNAs (miRNAs) in mediating intercellular signaling. METHODS: Microparticles (MP) from HUVEC and 293T were isolated by sequential centrifugation. THP-1 was co-cultured with microparticles. And then the migration of THP-1 was measured by transwell. real-time PCR and Western blotting were used to study the related mechanisms. RESULTS: Compared with the microparticles from 293T, MP from HUVEC could promote the migration of monocytes (P<0.05) and upregulate the expression of CXCR4 mRNA and protein (P<0.05). MiRNA-126 deficient MP could downregulate the migration of monocytes (P<0.05) and the expression of CXCR4 mRNA and protein (P<0.05) compared with miRNA-126 abundant MP. CONCLUSION: Microparticles from HUVEC could promote the migration of monocytes. As carriers, microparticles could mediate intercellular signaling.


Asunto(s)
MicroARNs/metabolismo , Monocitos/citología , Transducción de Señal , Línea Celular , Técnicas de Cocultivo , Regulación hacia Abajo , Células HEK293 , Células Endoteliales de la Vena Umbilical Humana , Humanos , ARN Mensajero , Receptores CXCR4/metabolismo , Regulación hacia Arriba
9.
Beijing Da Xue Xue Bao Yi Xue Ban ; 46(6): 911-6, 2014 Dec 18.
Artículo en Chino | MEDLINE | ID: mdl-25512282

RESUMEN

OBJECTIVE: To analyze the risk factors involved in the weaning from mechanical ventilation in critical patients who underwent major abdominal surgery. METHODS: This retrospective study was conducted at Department of Critical Care Medicine in Peking University People's Hospital. The subjects included all critical ill patients who underwent major abdominal surgery from January 2011 to December 2013. Clinical and laboratory parameters in perioperative period were investigated for the risk factors involved in the weaning from mechanical ventilation. RESULTS: In this study, 381 patients were included, of whom, 274 were successfully weaned. We found old age, lower left ventricular ejection fraction (LVEF) before surgery, with the complication of myocardial injury after noncardiac surgery (MINS) and lower serum albumin level after surgery were the independent risk factors of weaning from mechanical ventilation. And the days of intensive care unit and 28-day mortality of patients who successfully weaned were better than patients who failed to wean. CONCLUSION: Old age, lower LVEF before surgery, with the complication of MINS and lower serum albumin level after surgery were independent risk factors of weaning in critical patients who underwent major abdominal surgery.


Asunto(s)
Respiración Artificial , Procedimientos Quirúrgicos Operativos , Desconexión del Ventilador , Factores de Edad , Humanos , Unidades de Cuidados Intensivos , Estudios Retrospectivos , Factores de Riesgo , Albúmina Sérica , Factores de Tiempo , Función Ventricular Izquierda
10.
J Magn Reson ; 166(1): 82-91, 2004 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-14675823

RESUMEN

In this paper, we propose a novel approach for electron paramagnetic resonance (EPR) mixture spectra analysis based on blind source separation (BSS) technique. EPR spectrum of a free radical is often superimposed by overlapping spectra of other species. It is important and challenging to accurately identify and quantify the 'pure' spectra from such mixtures. In this study, an automated BSS method implementing independent component analysis is used to extract the components from mixed EPR spectra that contain overlapping components of different paramagnetic centers. To apply this method, there is no requirement to know the component spectra or the number of components in advance. The method is applied to analyze free radical EPR spectra which are collected from standard chemical system, cultured cell suspense, and ex vivo rat kidneys by spin trapping EPR technique. Results show that the BSS method proposed here is capable of identifying the component EPR spectra from mixtures with unknown compositions. The BSS technique can offer powerful aids in resolving spectral overlapping problems in general EPR spectroscopy analysis.


Asunto(s)
Algoritmos , Óxidos N-Cíclicos/química , Espectroscopía de Resonancia por Spin del Electrón/métodos , Radicales Libres/química , Especies Reactivas de Oxígeno/química , Procesamiento de Señales Asistido por Computador , Soluciones/química , Animales , Células CHO/química , Cricetinae , Cricetulus , Óxidos N-Cíclicos/análisis , Radicales Libres/análisis , Radical Hidroxilo/análisis , Radical Hidroxilo/química , Riñón/química , Óxido Nítrico/análisis , Óxido Nítrico/química , Especies Reactivas de Oxígeno/análisis , Soluciones/análisis , Superóxidos/análisis , Superóxidos/química
11.
Osteoporos Int ; 12(9): 755-62, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11605742

RESUMEN

Calcaneus bone mineral density (BMD) of 7428 Chinese (4126 women, 3302 men; aged 22-94 years) was measured using single-energy X-ray absorptiometry (SXA). A reference range of calcaneus BMD values for healthy Chinese men and women was established and the usefulness of this method for screening and diagnosis in osteoporosis was evaluated. The peak BMD occurred at 20-24 years old and peak BMD in women was significantly lower than in men. BMD loss in the calcaneus started at the age of 35 years for women, and at 63 years in men. BMD loss rate was 1.2%/year for women and 0.56%/year for men after 50 years. The young normal reference for calcaneus BMD was 442.1+/-69.6 mg/cm2 for men and 388.3+/-61.7 mg/cm2 for women calculated from the mean BMD value of subjects whose age ranged from 20 to 49 years. The accumulated BMD loss in the calcaneus is similar to that of Ward's triangle. Multiple linear regression showed that both age and weight were important factors. The incidence of osteoporosis in older men and women (> or = 60 years) is 6.6% and 32.1% respectively. We conclude that calcaneus BMD measurement is useful and sensitive for the screening and diagnosis of osteoporosis. A predictive diagnostic model for osteoporosis based on the calcaneus was constructed using multiple linear regression and the WHO criteria for diagnosing osteoporosis can be applied to calcaneus BMD.


Asunto(s)
Absorciometría de Fotón/normas , Densidad Ósea/fisiología , Tamizaje Masivo/normas , Osteoporosis/diagnóstico por imagen , Adulto , Anciano , Anciano de 80 o más Años , Calcáneo/fisiología , China/etnología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Modelos Teóricos , Cintigrafía , Valores de Referencia , Sensibilidad y Especificidad
12.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 21(9): 645-8, 2001 Sep.
Artículo en Chino | MEDLINE | ID: mdl-12575546

RESUMEN

OBJECTIVE: To evaluate the efficacy of Qinggan Granule (QGG) and Bushen Granule (BSG) in treating chronic hepatitis C (CHC) from blood donors clinically, biochemically and pathologically as well as to explore the therapeutical principle and methods of TCM. METHODS: Sixty-six patients with CHC were divided into three groups, the 36 patients in the QGG group treated with QGG, the 18 patients in the BSG group treated with BSG and the 12 patients in the control group untreated. The dose of QGG and BSG given was 30 g each time, three times per day for 6 consecutive months. Clinical and serum biochemical parameters as well as pathological change of liver biopsy before and after treatment were observed dynamically and compared. RESULTS: After treatment, in the two treated group, clinical symptoms were improved significantly, alanine transaminase (ALT) and aspartate aminotransferase (AST) reduced markedly. Moreover, QGG showed the effects of increasing albumin and lowering alkaline phosphatase (ALP). On the contrary, ALT still remained as before but AST further increased in the control group. Pathological examination showed that the inflammatory grade (IG) decreased in 42.2% and fibrotic stage (FS) decreased 21.2% of the patients in the QGG group, but no significant changes of the two indexes occurred in the BSG group, while in the control group, IG unchanged and FS increased in 3 cases. Chevallier's semi-quantity system analysis showed significant decrease of both scores in the QGG group (P < 0.05), only decrease of IG in the BSG group, and slight raise of IG and significant increase of FS (P < 0.05) in the control group. CONCLUSION: Condition of chronic hepatitis C patient would deteriorate progressively if not treated in time, especially the development of fibrosis. QGG and BSG could improve the clinical symptoms significantly, lower ALT and AST, eliminate inflammatory damage in the liver, slow down or reverse liver fibrosis process with stable long-term effect. Though most of the patients present a Syndrome of Liver-Kidney Yin-deficiency, the effect of QGG is superior to that of BSG, suggesting that the treatment of CHC should mainly be clearing principle, and method of clearing Liver and eliminating Dampness may be more suitable for them.


Asunto(s)
Medicamentos Herbarios Chinos/uso terapéutico , Hepatitis C Crónica/tratamiento farmacológico , Fitoterapia , Adulto , Donantes de Sangre , Femenino , Humanos , Hígado/patología , Hígado/fisiopatología , Masculino , Persona de Mediana Edad
13.
Growth Dev Aging ; 61(1): 19-26, 1997.
Artículo en Inglés | MEDLINE | ID: mdl-9129967

RESUMEN

To theoretically and quantitively describe the phenomena that bone mineral density (BMD) was changed with, the three-exponential model was proposed. Our results showed that BMD in radius and ulna in a sample of 36,879 healthy people ranging in age from 2 to 100 years fit the three-exponential equation well. Bone mass loss in Chinese women was faster than that in Chinese men. The method to accurately calculate the peak BMD and the corresponding age was given. The peak BMD and age for Chinese men and women in radius were 0.7469 g/cm2 and 35 years old, and 0.7051 g/cm2 and 33 years old respectively. The peak BMD and age in ulna were 0.7515 g/cm2 and 35 years old for Chinese men, and 0.6904 g/cm2 and 31 years old for Chinese women. Peak BMD in Chinese women was lower than in men. Peak BMD occurred slightly earlier in Chinese women. We concluded that our model could successfully characterize the regularity of bone mass accumulation and loss during the whole lifetime. The quantitive relationship was very useful in the diagnosis of osteoporosis.


Asunto(s)
Envejecimiento/fisiología , Densidad Ósea/fisiología , Simulación por Computador , Modelos Biológicos , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Niño , Preescolar , China/epidemiología , Femenino , Humanos , Modelos Lineales , Masculino , Persona de Mediana Edad , Osteoporosis/diagnóstico , Osteoporosis/epidemiología , Osteoporosis/fisiopatología , Radio (Anatomía)/fisiología , Cúbito/fisiología
14.
Zhongguo Yao Li Xue Bao ; 18(6): 515-8, 1997 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-10322908

RESUMEN

AIM: To study the effects of berberine (Ber) on L-(ICa,L) and T-type (ICa,T) channels in isolated guinea pig ventricular myocytes. METHODS: Using whole cell patch-clamp recording technique. RESULTS: Ber 10, 30 mumol.L-1 inhibited the ICa, L from 1400 +/- 247 pA to 978 +/- 204 pA (n = 5 cells of 5 guinea pigs, P < 0.05), and to 664 +/- 179 pA (n = 5, P < 0.01), respectively. The inhibitory effect was concentration-dependent and non-frequency-dependent. The peak value of ICa,L in the current-voltage relationship was decreased. Ber affected the inactivation kinetics of ICa,L. The half activation potential (V0.5) was shifted from -27.8 mV to -34.2 mV and the slope factor (kappa) was changed from 9.22 into 13.03. Ber did not affect the activation kinetics. Ber 10 and 30 mumol.L-1 also inhibited ICa,T (from 154 +/- 80 pA to 101 +/- 78 pA, and to 48 +/- 45 pA, n = 8 cells of 5 guinea pigs, P < 0.05). CONCLUSION: Ber possessed blocking effects on both L- and T-type calcium channels.


Asunto(s)
Antiarrítmicos/farmacología , Berberina/farmacología , Canales de Calcio/efectos de los fármacos , Miocardio/citología , Animales , Células Cultivadas , Cobayas , Ventrículos Cardíacos , Masculino , Técnicas de Placa-Clamp
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