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1.
Artículo en Inglés | MEDLINE | ID: mdl-31618373

RESUMEN

Santa Catarina is a Brazilian State that has reported the lowest prevalence of human T-cell lymphotropic viruses (HTLV-1/2) in blood donors (0.04%). Although it presents ports, airports and roads that facilitate the entrance and dissemination of new infectious agents, no information exists concerning the HTLV-1/2 infections in HIV/AIDS patients. This study searched for HTLV-1/2 antibodies in plasma samples of 625 HIV/AIDS patients from the municipality of Tubarao (Southern Santa Catarina), and disclosed 1.1% of positivity (0.48% HTLV-1, 0.48% HTLV-2 and 0.16% untypeable HTLV), and a positive correlation with the male sex (OR 4.16) and intravenous drug use (OR 35.18). Although the percentage of 1.1% appears to be low, it is 27.5 times higher than the percentage detected in blood donors. Since HTLV-1 and HTLV-2 are circulating in HIV-infected individuals in Southern Santa Catarina, and these retroviruses could cause a differently impact on the HIV/AIDS outcomes, the surveillance of HTLV-1/2 is necessary, and it could support public health policies in preventing the transmission and dissemination of these viruses in this State.


Asunto(s)
Anticuerpos Antivirales/sangre , Infecciones por VIH/epidemiología , Infecciones por HTLV-I/epidemiología , Infecciones por HTLV-II/epidemiología , Virus Linfotrópico T Tipo 1 Humano/inmunología , Virus Linfotrópico T Tipo 2 Humano/inmunología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Brasil/epidemiología , Estudios Transversales , Monitoreo Epidemiológico , Femenino , Infecciones por VIH/complicaciones , Infecciones por HTLV-I/complicaciones , Infecciones por HTLV-I/diagnóstico , Infecciones por HTLV-II/complicaciones , Humanos , Masculino , Persona de Mediana Edad , Prevalencia , Adulto Joven
2.
BrJP ; 1(4): 305-309, Oct.-Dec. 2018. tab, graf
Artículo en Inglés | LILACS-Express | ID: biblio-1038959

RESUMEN

ABSTRACT BACKGROUND AND OBJECTIVES: Musculoskeletal disorder is characterized by changes in neuromuscular, tendinous and joint functions that impact the functionality of the affected areas, especially with pain. The intervention with reflexotherapy is a non-invasive alternative and allows instant control of acute occupational low back pain. The objective of this study was to describe the results of foot reflexology in the relief of acute low back pain related to the nursing staff job. METHODS: Double-blind controlled clinical trial. Thirty-six effective participants were randomly assigned to experimental and control group. Reflexotherapy was applied for low back pain in the sample groups in two sessions with a 72-hour interval. Data collection was performed with the visual analog scale to assess pain before and after the interventions, and a questionnaire for low back pain at the beginning and end of the study. The data were analyzed with the following tests: Shapiro Wilk, for sample normality; Chi-square or Fisher's Exact, for the association ­between categorical variables and t-test. RESULTS: There was statistical significance with the foot reflexology in the relief of acute low back pain, with p=0.000 for the visual analog scale and the data of the questionnaire for low back pain p=0.0002. CONCLUSION: Reflexotherapy showed to be effective in reducing the intensity of acute pain among nursing professionals in this study. The Brazilian Registry of Clinical Trials is RBR-5ndq22.


RESUMO JUSTIFICATIVA E OBJETIVOS: O distúrbio osteomuscular é caracterizado por alterações nas funções neuromusculares, tendinosas e articulares, que provocam impacto na funcionalidade da área afetada e, principalmente, dor. A intervenção com a reflexoterapia é uma alternativa não invasiva que possibilita controle imediato da dor lombar aguda ocupacional. O objetivo deste estudo foi descrever os resultados da reflexoterapia podal no alívio da dor lombar aguda relacionada ao trabalho da equipe de enfermagem. MÉTODOS: Ensaio clínico controlado, duplamente encoberto. Trinta e seis participantes efetivos foram randomicamente alocados em grupo experimental e controle. Aplicou-se a reflexoterapia para dor lombar nos grupos amostrais em duas sessões, com intervalo de 72 horas. A coleta de dados foi realizada com a escala analógica visual de intensidade na avaliação da dor, antes e depois das intervenções e questionário para lombalgia, no início e fim da pesquisa. Os dados foram analisados com os testes Shapiro Wilk, para normalidade da amostra; Qui-quadrado ou Exato de Fisher, para associação das variáveis categóricas e teste t. RESULTADOS: Houve significância estatística com a reflexoterapia podal no alívio da dor lombar aguda, obtendo-se p=0,000, para a escala analógica visual, e nos dados do questionário para lombalgia p=0,0002. CONCLUSÃO: A reflexoterapia mostrou-se eficaz na redução da intensidade dador lombar aguda dos profissionais de enfermagem, neste estudo. O Registro Brasileiro de Ensaios Clínicos é RBR-5ndq22.

3.
ACM arq. catarin. med ; 47(3): 116-132, jul.-set. 2018.
Artículo en Portugués | LILACS-Express | ID: biblio-915954

RESUMEN

O objetivo do estudo é analisar a tendência temporal da incidência de leptospirose, de acordo com índices pluviométricos na Região da Grande Florianópolis, conforme sazonalidade - 2005 a 2015. Trata-se de estudo ecológico de séries temporais, com dados de leptospirose, índices pluviométricos e população. Foram analisadas taxas de incidência de leptospirose, excesso relativo da incidência, coeficiente de correlação de Pearson (r), coeficiente angular (ß), a partir do ajuste da regressão linear, com nível de significância de 95%. Apresenta distribuição dos casos de leptospirose, índices pluviométricos e razão casos/índices pluviométricos, estratificados por mês de ocorrência. Foram registrados 1.001 casos de leptospirose, média mensal de 83 casos, com variação de 43, nos meses de agosto, a 146, nos meses de fevereiro. A taxa média de leptospirose foi 8,24/100 mil habitantes. O índice pluviométrico médio foi 154,83 mm, os índices mais baixos ocorreram nos meses de junho, média de 99,48 mm, e, os mais elevados nos meses de janeiro, média de 226,16 mm. Uma correlação positiva entre taxas de leptospirose e níveis pluviométricos, ao longo do período, janeiro a dezembro (r=0,64; p=0,003), aponta para associação temporal positiva entre quantidade de chuva e casos da doença. A doença ocorreu o ano todo e apresentou nítida sazonalidade no período de outubro a março.

4.
Rev Inst Med Trop Sao Paulo ; 60: e39, 2018 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-30066807

RESUMEN

The objective of the study is to analyze the temporal trend of leptospirosis incidence, according to rainfall levels in Santa Catarina, according to seasonality, from 2005 to 2015. This is an ecological study of time series, with date of leptospirosis, rainfall levels and population. The incidence rates of leptospirosis, relative excess of incidence, Pearson's correlation coefficient (r) and an angular coefficient (ß) were analyzed from the linear regression adjustment, with a 5% significance level. Distribution of leptospirosis cases, rainfall levels and cases reason/rainfall levels, stratified by month of occurrence were presented. There were 5,274 cases of leptospirosis, with the monthly average being 439 cases, ranging from 211 in September to 770 in January. The mean rate of leptospirosis was 7.03 per 100,000 habitants. The average rainfall level was 158.68 mm, with the lowest levels occurring in August, average of 124.9 mm, and the highest in January average of 213.20 mm. The positive correlation between leptospirosis rates and rainfall levels, during the period from January to December (r = 0.68, p = 0.023), indicates a positive temporal association between the amount of rainfall and the cases of the disease. The disease occurred all year round and presented a distinct seasonality from October to March.


Asunto(s)
Leptospirosis/epidemiología , Lluvia , Estaciones del Año , Brasil/epidemiología , Humanos , Incidencia
5.
Mem. Inst. Oswaldo Cruz ; 109(3): 356-361, 06/2014. tab, graf
Artículo en Inglés | LILACS | ID: lil-711732

RESUMEN

The identification of mycobacteria is essential because tuberculosis (TB) and mycobacteriosis are clinically indistinguishable and require different therapeutic regimens. The traditional phenotypic method is time consuming and may last up to 60 days. Indeed, rapid, affordable, specific and easy-to-perform identification methods are needed. We have previously described a polymerase chain reaction-based method called a mycobacteria mobility shift assay (MMSA) that was designed for Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM) species identification. The aim of this study was to assess the MMSA for the identification of MTC and NTM clinical isolates and to compare its performance with that of the PRA-hsp65 method. A total of 204 clinical isolates (102 NTM and 102 MTC) were identified by the MMSA and PRA-hsp65. For isolates for which these methods gave discordant results, definitive species identification was obtained by sequencing fragments of the 16S rRNA and hsp65 genes. Both methods correctly identified all MTC isolates. Among the NTM isolates, the MMSA alone assigned 94 (92.2%) to a complex or species, whereas the PRA-hsp65 method assigned 100% to a species. A 91.5% agreement was observed for the 94 NTM isolates identified by both methods. The MMSA provided correct identification for 96.8% of the NTM isolates compared with 94.7% for PRA-hsp65. The MMSA is a suitable auxiliary method for routine use for the rapid identification of mycobacteria.


Asunto(s)
Humanos , Ensayo de Cambio de Movilidad Electroforética , Mycobacterium tuberculosis/aislamiento & purificación , Micobacterias no Tuberculosas/aislamiento & purificación , /genética , Técnicas de Tipificación Bacteriana , Proteínas Bacterianas/genética , ADN Bacteriano/genética , Infecciones por Micobacterias no Tuberculosas/microbiología , Infecciones por Mycobacterium/microbiología , Mycobacterium tuberculosis/clasificación , Micobacterias no Tuberculosas/clasificación , Reacción en Cadena de la Polimerasa
6.
Mem Inst Oswaldo Cruz ; 109(3): 356-61, 2014 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-24821059

RESUMEN

The identification of mycobacteria is essential because tuberculosis (TB) and mycobacteriosis are clinically indistinguishable and require different therapeutic regimens. The traditional phenotypic method is time consuming and may last up to 60 days. Indeed, rapid, affordable, specific and easy-to-perform identification methods are needed. We have previously described a polymerase chain reaction-based method called a mycobacteria mobility shift assay (MMSA) that was designed for Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM) species identification. The aim of this study was to assess the MMSA for the identification of MTC and NTM clinical isolates and to compare its performance with that of the PRA-hsp65 method. A total of 204 clinical isolates (102 NTM and 102 MTC) were identified by the MMSA and PRA-hsp65. For isolates for which these methods gave discordant results, definitive species identification was obtained by sequencing fragments of the 16S rRNA and hsp65 genes. Both methods correctly identified all MTC isolates. Among the NTM isolates, the MMSA alone assigned 94 (92.2%) to a complex or species, whereas the PRA-hsp65 method assigned 100% to a species. A 91.5% agreement was observed for the 94 NTM isolates identified by both methods. The MMSA provided correct identification for 96.8% of the NTM isolates compared with 94.7% for PRA-hsp65. The MMSA is a suitable auxiliary method for routine use for the rapid identification of mycobacteria.


Asunto(s)
Ensayo de Cambio de Movilidad Electroforética , Mycobacterium tuberculosis/aislamiento & purificación , Micobacterias no Tuberculosas/aislamiento & purificación , ARN Ribosómico 16S/genética , Proteínas Bacterianas/genética , Técnicas de Tipificación Bacteriana , ADN Bacteriano/genética , Humanos , Infecciones por Mycobacterium/microbiología , Infecciones por Micobacterias no Tuberculosas/microbiología , Mycobacterium tuberculosis/clasificación , Micobacterias no Tuberculosas/clasificación , Reacción en Cadena de la Polimerasa
7.
Nucleic Acids Res ; 41(15): 7387-400, 2013 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-23761445

RESUMEN

Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vector-human and vector-parasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible at www.labinfo.lncc.br/index.php/anopheles-darlingi.


Asunto(s)
Anopheles/genética , Genoma de los Insectos , Insectos Vectores/genética , Animales , Anopheles/clasificación , Brasil , Cromosomas de Insectos/genética , Elementos Transponibles de ADN , Evolución Molecular , Femenino , Variación Genética , Interacciones Huésped-Parásitos , Proteínas de Insectos/genética , Insectos Vectores/clasificación , Resistencia a los Insecticidas , Insecticidas/farmacología , Malaria/parasitología , Masculino , Anotación de Secuencia Molecular , Filogenia , Sintenía , Transcriptoma
8.
Fish Shellfish Immunol ; 35(2): 319-27, 2013 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-23664909

RESUMEN

Perkinsus genus includes protozoan parasites of marine mollusks, especially bivalves. In the last four years, this parasite has been detected in mangrove oysters Crassostrea rhizophorae and Crassostrea gasar from the Northeastern region of Brazil. Hemocytes are the key cells of the oyster immune system, being responsible for a variety of cellular and humoral reactions, such as phagocytosis, encapsulation and the release of several effector molecules that control the invasion and proliferation of microorganisms. In Brazil, there is little information on perkinsosis and none on the immune responses of native oysters' species against Perkinsus spp. The objective of this study was to determine the effects of natural infection by Perkinsus sp. on the immunological parameters of mangrove oysters C. gasar cultured in the Mamanguape River Estuary (Paraíba, Brazil). Adults oysters (N = 40/month) were sampled in December 2011, March, May, August and October 2012. Gills were removed and used to determine the presence and intensity of the Perkinsus sp. infection, according to a scale of four levels (1-4), using the Ray's fluid thioglycollate medium assay. Immunological parameters were measured in hemolymph samples by flow cytometry, including: total hemocyte count (THC), differential hemocyte count (DHC), cell mortality, phagocytic capacity, and production of Reactive Oxygen Species (ROS). The plasma was used to determine the hemagglutination activity. The results showed the occurrence of Perkinsus sp. with the highest mean prevalence (93.3%) seen so far in oyster populations in Brazil. Despite that, no oyster mortality was associated. In contrast, we observed an increase in hemocyte mortality and a suppression of two of the main defense mechanisms, phagocytosis and ROS production in infected oysters. The increase in the percentage of blast-like cells on the hemolymph, and the increase in THC in oysters heavily infected (at the maximum intensity, 4) suggest an induction of hemocytes proliferation. The immunological parameters varied over the studied months, which may be attributed to the dynamics of infection by Perkinsus sp. The results of the present study demonstrate that Perkinsus sp. has a deleterious effect on C. gasar immune system, mainly in high intensities, which likely renders oysters more susceptible to other pathogens and diseases.


Asunto(s)
Alveolados/fisiología , Crassostrea/inmunología , Crassostrea/microbiología , Animales , Acuicultura , Brasil , Estuarios , Citometría de Flujo/veterinaria , Hemocitos/citología , Hemocitos/metabolismo , Fagocitosis , Especies Reactivas de Oxígeno/metabolismo , Estaciones del Año
9.
J Proteomics ; 82: 52-63, 2013 Apr 26.
Artículo en Inglés | MEDLINE | ID: mdl-23466310

RESUMEN

UNLABELLED: Sympatric distribution and sharing of hosts and antigens by Trypanosoma rangeli and Trypanosoma cruzi, the etiological agent of Chagas' disease, often incur in misdiagnosis and improper epidemiological inferences. Many secreted and surface proteins (SP) have been described as important antigens shared by these species. This work describes the T. rangeli surfaceome obtained by gel-free (LC-ESI-MS/MS) and gel-based (GeLC-ESI-MS/MS) proteomic approaches, and immunoblotting analyses and the comparison of these SP with T. cruzi. A total of 138 T. rangeli proteins and 343 T. cruzi proteins were obtained, among which, 42 and 157 proteins were exclusively identified in T. rangeli or T. cruzi trypomastigotes, respectively. Immunoblotting assays using sera from experimentally infected mice revealed a distinct band pattern for each species. MS/MS analysis of T. rangeli exclusive bands revealed two unique GP63-related proteins and flagellar calcium-binding protein. Also, a ~32kDa band composed of 12 distinct proteins was exclusively recognized by anti-T. cruzi serum. This highly sensitive proteomic assessment of surface proteins characterized the T. rangeli surfaceome, revealing several differences and similarities between these two parasites. The study reports new T. rangeli-specific proteins with promising use in differential diagnosis from T. cruzi. BIOLOGICAL SIGNIFICANCE: In this manuscript, we report the first proteomic analysis of the T. rangeli surface (surfaceome), a non-pathogenic parasite occurring in sympatry with T. cruzi, the etiological agent of Chagas disease. This comparative proteomic analysis was performed using high-throughput in-gel and gel-free proteomic approaches combined with immunoblotting, allowing us to identify new T. rangeli-specific proteins with promising use in differential serodiagnosis, among several other protein not previously reported for this taxon. Additionally, cross-recognition assays showed that T. cruzi surface proteins were recognized by heterologous serum (anti-T. rangeli) that strengthens the possibility of misdiagnosis of Chagas disease in humans and other mammals. Thus, this work provides new insights to understand the serological cross-reactivity between T. cruzi and T. rangeli, as well as, the identification of targets for specific T. rangeli diagnosis as revealed by the comparative surfaceome analysis. We strongly believe that this research is of importance to the readers of Journal of Proteomics since it provides new potential markers for diagnosis of both T. cruzi and T. rangeli parasites increasing the spectrum of specific targets for unambiguous diagnosis of T. rangeli and T. cruzi infections, besides describing new approaches to assess the trypanosomatids proteome.


Asunto(s)
Proteómica , Proteínas Protozoarias/metabolismo , Trypanosoma rangeli/metabolismo , Tripanosomiasis/metabolismo , Animales , Humanos , Ratones , Pruebas Serológicas/métodos , Tripanosomiasis/diagnóstico
10.
Artículo en Inglés | MEDLINE | ID: mdl-22169266

RESUMEN

The effects of hyposmotic stress and white spot syndrome virus (WSSV) challenge in expression was studied in the marine shrimp Litopenaeus vannamei. Messenger RNA from gills of shrimp submitted to osmotic stress was isolated to identify genes differentially expressed through the suppressive subtractive hybridization (SSH) method. Two subtractive libraries forward and two reverse were constructed to identify up and down-regulated genes under these conditions. About 192 clones were sequenced, of which 46 genes were identified. These genes encode proteins corresponding to a wide range of biological roles, including defense, cell signaling, electron transfer, cell proliferation and differentiation, apoptosis, intermediary metabolism, cytoskeleton and digestion. Among the identified genes, 19 were up-regulated and 27 were down-regulated in the animals kept at a lower ion concentration. We evaluated the expression of eight genes by RT-qPCR in shrimp submitted to hyposmotic conditions with and without WSSV challenge. The SSH enabled the identification of genes that are influenced by hyposmotic stress. A significant up-regulation was observed in lectin-C, QM, TGF beta inducible nuclear protein 1, ciclophilin, malate dehydrogenase, mitochondrial ATP synthase F chain and ATP synthase subunit 9 precursor transcripts. However, the expression of these genes in L. vannamei was not affected by WSSV infection both at isosmotic and hyposmotic conditions.


Asunto(s)
Penaeidae/genética , Penaeidae/virología , Virus del Síndrome de la Mancha Blanca 1/fisiología , Animales , Perfilación de la Expresión Génica , Regulación de la Expresión Génica , Interacciones Huésped-Patógeno , Presión Osmótica , Penaeidae/fisiología
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