Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 20
Filtrar
Más filtros










Base de datos
Intervalo de año de publicación
1.
iScience ; 24(11): 103311, 2021 Nov 19.
Artículo en Inglés | MEDLINE | ID: mdl-34778730

RESUMEN

Neurotransmitter receptors are involved in cancer progression. Among them, the heterodimeric GABAB receptor, activated by the main inhibitory neurotransmitter GABA, is composed of the transmembrane GABAB1 and GABAB2 subunits. The oncogenic role of the isoform GABAB1e (GB1e) containing only the extracellular domain of GABAB1 remains unclear. We revealed that GB1e is largely expressed in human breast cancer (BrCa) cell lines as well as in BrCa tissues where it is upregulated. Moreover, GB1e promoted the malignancy of BrCa cells both in vitro and in vivo. We propose that GB1e favors EGFR signaling by interacting with PTPN12 to disrupt the interaction between EGFR and PTPN12, and phosphorylation of Y230 and Y404 on GB1e is required in this process. Our data highlight that the GABBR1 gene through the expression of the GB1e isoform might play an important oncogenic role in BrCa and that GB1e is of interest for the treatment of some cancers.

2.
J Environ Sci (China) ; 110: 38-47, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34593193

RESUMEN

To assess groundwater nitrate contamination and its human health risks, 489 unconfined groundwater samples were collected and analyzed from Zhangjiakou, northern China. The spatial distribution of principle hydrogeochemical results showed that the average concentrations of ions in descend order was HCO3-, SO42-, Na+, Ca2+, Cl-, NO3-, Mg2+ and K+, among which the NO3- concentrations were between 0.25 and 536.73 mg/L with an average of 29.72 mg/L. In total, 167 out of 489 samples (~ 34%) exceeded the recommended concentration of 20 mg/L in Quality Standard for Groundwater of China. The high NO3- concentration groundwater mainly located in the northern part and near the boundary of the two geomorphic units. As revealed by statistical analysis, the groundwater chemistry was more significantly affected by anthropogenic sources than by the geogenic sources. Moreover, human health risks of groundwater nitrate through oral and dermal exposure pathways were assessed by model, the results showed that about 60%, 50%, 32% and 26% of the area exceeded the acceptable level (total health index>1) for infants, children, adult males and females, respectively. The health risks for different groups of people varied significantly, ranked: infants> children> adult males>adult females, suggesting that younger people are more susceptible to nitrate contamination, while females are more resistant to nitrate contamination than males. To ensure the drinking water safety in Zhangjiakou and its downstream areas, proper management and treatment of groundwater will be necessary to avoid the health risks associated with nitrate contamination.


Asunto(s)
Agua Subterránea , Contaminantes Químicos del Agua , China , Monitoreo del Ambiente , Femenino , Humanos , Masculino , Nitratos/análisis , Medición de Riesgo , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidad
3.
Mol Med Rep ; 24(2)2021 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-34080028

RESUMEN

Dysregulation of long non­coding RNA (IncRNA) antisense non­coding RNA in the INK4 locus (ANRIL) is associated with the risk of myocardial infarction (MI). Therefore, the present study aimed to determine the mechanisms underlying this association, which is currently poorly understood, to the best of our knowledge. The current study used an in vitro myocardial ischemia and reperfusion (MI/R) model, in which H9c2 cardiomyocytes were exposed to hypoxia/reoxygenation (H/R), which demonstrated that ANRIL expression was downregulated and that ANRIL positively regulated sirtuin 1 (SIRT1) expression following H/R injury. Subsequently, it was demonstrated that ANRIL upregulated SIRT1 expression by sponging microRNA­181a (miR­181a). In addition, ANRIL overexpression reduced lactate dehydrogenase release and apoptosis of H9c2 cardiomyocytes exposed to H/R, indicating that ANRIL prevented H/R­induced cardiomyocyte injury. Moreover, both miR­181a overexpression and SIRT1 knockdown significantly decreased the protective effects of ANRIL on H/R­induced cardiomyocyte injury, thus demonstrating that SIRT1 upregulation via sponging miR­181a is a critical mechanism that mediates the function of ANRIL. These results provided a novel mechanistic insight into the role of ANRIL in H/R­injured cardiomyocytes and suggested that the ANRIL/miR­181a/SIRT1 axis may be a therapeutic target for reducing MI/R injury.


Asunto(s)
Daño por Reperfusión Miocárdica/genética , Daño por Reperfusión Miocárdica/metabolismo , Miocitos Cardíacos/metabolismo , ARN Largo no Codificante/genética , ARN Largo no Codificante/metabolismo , Sirtuina 1/genética , Sirtuina 1/metabolismo , Animales , Apoptosis/genética , Hipoxia de la Célula/genética , Línea Celular , Regulación hacia Abajo/genética , MicroARNs/metabolismo , Modelos Biológicos , Isquemia Miocárdica/genética , Isquemia Miocárdica/metabolismo , Isquemia Miocárdica/prevención & control , Daño por Reperfusión Miocárdica/prevención & control , Oxidación-Reducción , Ratas , Regulación hacia Arriba/genética
4.
Plant Cell ; 32(10): 3124-3138, 2020 10.
Artículo en Inglés | MEDLINE | ID: mdl-32796126

RESUMEN

Circadian clocks regulate growth and development in plants and animals, but the role of circadian regulation in crop production is poorly understood. Rice (Oryza sativa) grain yield is largely determined by tillering, which is mediated by physiological and genetic factors. Here we report a regulatory loop that involves the circadian clock, sugar, and strigolactone (SL) pathway to regulate rice tiller-bud and panicle development. Rice CIRCADIAN CLOCK ASSOCIATED1 (OsCCA1) positively regulates expression of TEOSINTE BRANCHED1 (OsTB1, also known as FC1), DWARF14 (D14), and IDEAL PLANT ARCHITECTURE1 (IPA1, also known as OsSPL14) to repress tiller-bud outgrowth. Downregulating and overexpressing OsCCA1 increases and reduces tiller numbers, respectively, whereas manipulating PSEUDORESPONSE REGULATOR1 (OsPPR1) expression results in the opposite effects. OsCCA1 also regulates IPA1 expression to mediate panicle and grain development. Genetic analyses using double mutants and overexpression in the mutants show that OsTB1, D14, and IPA1 act downstream of OsCCA1 Sugars repress OsCCA1 expression in roots and tiller buds to promote tiller-bud outgrowth. The circadian clock integrates sugar responses and the SL pathway to regulate tiller and panicle development, providing insights into improving plant architecture and yield in rice and other cereal crops.


Asunto(s)
Relojes Circadianos/fisiología , Compuestos Heterocíclicos con 3 Anillos/metabolismo , Lactonas/metabolismo , Oryza/crecimiento & desarrollo , Proteínas de Plantas/genética , Azúcares/metabolismo , Regulación de la Expresión Génica de las Plantas , Mutación , Oryza/genética , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Plantas Modificadas Genéticamente , Regiones Promotoras Genéticas , Transducción de Señal
6.
Front Plant Sci ; 8: 1935, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-29170677

RESUMEN

Strigolactones (SLs) are the latest confirmed phytohormones that regulate shoot branching by inhibiting bud outgrowth in higher plants. Perception of SLs depends on a novel mechanism employing an enzyme-receptor DWARF14 (D14) that hydrolyzes SLs and becomes covalently modified. This stimulates the interaction between D14 and D3, leading to the ubiquitination and degradation of the transcriptional repressor protein D53. However, the regulation of SL perception in rice remains elusive. In this study, we provide evidences that D14 is ubiquitinated after SL treatment and degraded through the 26S proteasome system. The Lys280 site of the D14 amino acid sequence was important for SL-induced D14 degradation, but did not change the subcellular localization of D14 nor disturbed the interaction between D14 and D3, nor D53 degradation. Biochemical and genetic analysis indicated that the key amino acids in the catalytic center of D14 were essential for D14 degradation. We further showed that D14 degradation is dependent on D3 and is tightly correlated with protein levels of D53. These findings revealed that D14 degradation takes place following D53 degradation and functions as an important feedback regulation mechanism of SL perception in rice.

7.
Water Sci Technol ; 76(7-8): 1915-1924, 2017 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-28991806

RESUMEN

With an efficient methodology, a novel chloromethylated polystyrene-g-2-mercapto-1,3,4-thiadiazole chelating resin (MTR resin) was prepared via a one-step reaction. The structure of MTR resin was characterized by elements analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, and scanning electron microscopy. Meanwhile, the adsorption properties of the resin for Hg(II) were investigated by batch and column experiments. The results showed that the resin possessed much better adsorption capability for Hg(II) than for other metal ions. The statically and the dynamic saturated adsorption capacities were 343.8 mg/g and 475.1 mg/g. The adsorption kinetic and equilibrium data were well fitted to the second-order model and the Langmuir isotherm model, respectively. Desorption of mercury from the resin can be achieved using 30 mL of 2 mol/L HCl-5% thiourea solution with a desorption ratio of 92.3%. Compared with other absorbents, MTR resin was greatly conserve natural resources and reduce the cost.


Asunto(s)
Mercurio/química , Poliestirenos/química , Agua/química , Adsorción , Quelantes/química , Concentración de Iones de Hidrógeno , Cinética , Microscopía Electrónica de Rastreo , Soluciones , Espectroscopía Infrarroja por Transformada de Fourier , Termogravimetría , Contaminantes Químicos del Agua/química
8.
Cell Res ; 27(9): 1128-1141, 2017 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-28809396

RESUMEN

Strigolactones (SLs), a group of carotenoid derived terpenoid lactones, are root-to-shoot phytohormones suppressing shoot branching by inhibiting the outgrowth of axillary buds. DWARF 53 (D53), the key repressor of the SL signaling pathway, is speculated to regulate the downstream transcriptional network of the SL response. However, no downstream transcription factor targeted by D53 has yet been reported. Here we report that Ideal Plant Architecture 1 (IPA1), a key regulator of the plant architecture in rice, functions as a direct downstream component of D53 in regulating tiller number and SL-induced gene expression. We showed that D53 interacts with IPA1 in vivo and in vitro and suppresses the transcriptional activation activity of IPA1. We further showed that IPA1 could directly bind to the D53 promoter and plays a critical role in the feedback regulation of SL-induced D53 expression. These findings reveal that IPA1 is likely one of the long-speculated transcription factors that act with D53 to mediate the SL-regulated tiller development in rice.


Asunto(s)
Lactonas/farmacología , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Proteínas Represoras/metabolismo , Transducción de Señal , Factores de Transcripción/metabolismo , Retroalimentación Fisiológica , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Mutación con Pérdida de Función , MicroARNs/genética , MicroARNs/metabolismo , Oryza/efectos de los fármacos , Oryza/genética , Fenotipo , Regiones Promotoras Genéticas/genética , Unión Proteica/efectos de los fármacos , ARN Mensajero/genética , ARN Mensajero/metabolismo , Transducción de Señal/efectos de los fármacos , Transactivadores/metabolismo , Transcripción Genética/efectos de los fármacos , Activación Transcripcional/efectos de los fármacos , Activación Transcripcional/genética
10.
Plant Signal Behav ; 12(3): e1257455, 2017 03 04.
Artículo en Inglés | MEDLINE | ID: mdl-28402719

RESUMEN

Brassinosteroids (BRs) are a kind of plant hormones that regulate many important agronomic traits. Although the framework of the BR signaling pathway has been outlined, detailed working mechanisms at several steps of this pathway, especially at the BR receptor level, need to be further elucidated. Recently we have reported that a rice small G protein, OsPRA2, bound to the BR receptor OsBRI1 at the plasma membrane (PM) and inhibited its kinase activity and its interaction with the co-receptor OsBAK1, leading to a lower sensitivity to BR treatment and dephosphorylation of OsBZR1. In this follow-up study, we identified an OsPRA2-interacting protein through yeast 2 hybrid system, which is annotated as a C2-domain containing GTPase activating protein (OsGAP1). Overexpression of OsGAP1 in Arabidopsis resulted in a phenotype mimic to BR-deficiency, implying that the GTPase activity of OsPRA2 is also involved in regulating BR signaling. In addition, we observed that, besides colocalizing at the PM, OsPRA2 was also colocalized with OsBRI1 in small granules near PM, suggesting that the function of OsPRA2 in vesicle traffic may contribute to the regulation of OsBRI1.


Asunto(s)
Brasinoesteroides/metabolismo , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Membrana Celular/genética , Membrana Celular/metabolismo , Regulación de la Expresión Génica de las Plantas/genética , Regulación de la Expresión Génica de las Plantas/fisiología , Oryza/genética , Proteínas de Plantas/genética , Transducción de Señal/genética , Transducción de Señal/fisiología
11.
Biol Open ; 5(11): 1727-1734, 2016 Nov 15.
Artículo en Inglés | MEDLINE | ID: mdl-27659688

RESUMEN

The Cucumber mosaic virus (CMV) suppressor 2b co-localizes with AGO4 in cytoplasmic and nuclear fractions of Arabidopsis thaliana Biochemical fractionation of A. thaliana cellular extracts revealed that 2b and AGO4 coexist in multiple size exclusions. 2b transgenic A. thaliana exhibited an enhanced accumulation of 24nt siRNAs from flowering wageningen (FWA) and other heterochromatic loci. These plants also exhibited hypo-methylation of an endogenous- as well as transgene-FWA promoter at non-CG sites. In corroboration, both transgenic 2b and CMV infection affected the regulation of transposons which mimics the ago4 phenotype. In conclusion, 2b perturbs plant defense by interfering with AGO4-regulated transcriptional gene silencing.

12.
Mol Plant ; 9(9): 1260-1271, 2016 09 06.
Artículo en Inglés | MEDLINE | ID: mdl-27375203

RESUMEN

Brassinosteroids (BRs) are a class of steroid hormones that are essential for plant growth and development. The BR signal transduction pathway in the dicot model plant Arabidopsis is well established, but the components connecting the BR signaling steps in rice have not been fully explored. For example, how the BR signaling is fine-tuned in rice, especially at the BR receptor level, is largely unknown. Here we show that OsPRA2, a rice small G protein, plays a repressive role in the BR signaling pathway. Lamina inclination, coleoptile elongation, and root inhibition assays indicated that rice plants with suppressed expression of OsPRA2 were more sensitive to exogenously applied brassinolide than the wild-type plants. Conversely, rice overexpressing OsPRA2 was less sensitive to exogenous brassinolide. Further study uncovered that OsPRA2 inhibited the dephosphorylation of, and thus inactivated the transcription factor BRASSINAZOLE-RESISTANT 1 (OsBZR1). More importantly, OsPRA2 was found to co-localize with and directly bind to rice BR receptor BRASSINOSTEROID-INSENSITIVE 1 (OsBRI1) at the plasma membrane. Additionally, the in vitro assays showed that OsPRA2 inhibits its autophosphorylation. This OsPRA2-OsBRI1 interaction led to the dissociation of OsBRI1 from its co-receptor OsBAK1, and abolished OsBRI1-mediated phosphorylation of OsBAK1. Together, these results reveal a possible working mechanism of OsPRA2 as a novel negative regulator on OsBRI1 and OsBZR1 and extend the knowledge about the regulatory mechanism of rice BR signaling.


Asunto(s)
Proteínas de Unión al GTP Monoméricas/metabolismo , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Brasinoesteroides/metabolismo , Regulación de la Expresión Génica de las Plantas , Transducción de Señal
13.
PLoS One ; 9(5): e97410, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24819752

RESUMEN

BACKGROUND: RNA silencing is an important mechanism for regulation of endogenous gene expression and defense against genomic intruders in plants. This natural defense system was adopted to generate virus-resistant plants even before the mechanism of RNA silencing was unveiled. With the clarification of that mechanism, transgenic antiviral plants were developed that expressed artificial virus-specific hairpin RNAs (hpRNAs) or microRNAs (amiRNAs) in host plants. Previous works also showed that plant-mediated RNA silencing technology could be a practical method for constructing insect-resistant plants by expressing hpRNAs targeting essential genes of insects. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we chose aphid Myzus persicae of order Hemiptera as a target insect. To screen for aphid genes vulnerable to attack by plant-mediated RNA silencing to establish plant aphid resistance, we selected nine genes of M. persicae as silencing targets, and constructed their hpRNA-expressing vectors. For the acetylcholinesterase 2 coding gene (MpAChE2), two amiRNA-expressing vectors were also constructed. The vectors were transformed into tobacco plants (Nicotiana tabacum cv. Xanti). Insect challenge assays showed that most of the transgenic plants gained aphid resistance, among which those expressing hpRNAs targeting V-type proton ATPase subunit E-like (V-ATPaseE) or tubulin folding cofactor D (TBCD) genes displayed stronger aphicidal activity. The transgenic plants expressing amiRNAs targeting two different sites in the MpAChE2 gene exhibited better aphid resistance than the plants expressing MpAChE2-specific hpRNA. CONCLUSIONS/SIGNIFICANCE: Our results indicated that plant-mediated insect-RNA silencing might be an effective way to develop plants resistant to insects with piercing-sucking mouthparts, and both the selection of vulnerable target genes and the biogenetic type of the small RNAs were crucial for the effectiveness of aphid control. The expression of insect-specific amiRNA is a promising and preferable approach to engineer plants resistant to aphids and, possibly, to other plant-infesting insects.


Asunto(s)
Áfidos/fisiología , Ingeniería Genética , Secuencias Invertidas Repetidas , MicroARNs/genética , ARN de Planta/genética , Acetilcolinesterasa/deficiencia , Acetilcolinesterasa/genética , Animales , Áfidos/enzimología , Áfidos/genética , Clonación Molecular , Plantas Modificadas Genéticamente , Interferencia de ARN , Tabaco/genética , Tabaco/fisiología
14.
Huan Jing Ke Xue ; 34(10): 3879-87, 2013 Oct.
Artículo en Chino | MEDLINE | ID: mdl-24364306

RESUMEN

A micro power turbine water aeration system with a water biological base and a sediment biological base was independently developed, aimed at urban water eutrophication. The results showed that the average removal rates of COD, NH+4 -N, TP by the water biological base were 82. 33% , 98. 00% and 54. 73% , respectively; The sediment reduction rate achieved by the sediment biological base could reach 20% within 5 days, and aeration in the overlying water could relieve the nutrient releasing caused by the degradation of organic matter; The effect of nutrient removal and organic matter reduction in sediment by the combined ecological restoration technology was perfect in pilot scale. The average removal rates of COD, NH+4 -N, TP were 52. 0%, 33. 6% and 23.4%, respectively, and the organic content in sediment was reduced from 38. 20% to 12.20% .


Asunto(s)
Agua Dulce/química , Sedimentos Geológicos/química , Purificación del Agua/métodos , Compuestos de Amonio/análisis , Análisis de la Demanda Biológica de Oxígeno , Ciudades , Eutrofización , Nitrógeno/análisis , Fósforo/análisis , Contaminantes Químicos del Agua/análisis
15.
Mol Plant Microbe Interact ; 26(8): 927-36, 2013 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-23634838

RESUMEN

The P6 protein of Rice yellow stunt rhabdovirus (RYSV) is a virion structural protein that can be phosphorylated in vitro. However its exact function remains elusive. We found that P6 enhanced the virulence of Potato virus X (PVX) in Nicotiana benthamiana and N. tabacum plants, suggesting that it might function as a suppressor of RNA silencing. We examined the mechanism of P6-mediated silencing suppression by transiently expressing P6 in both N. benthamiana leaves and rice protoplasts. Our results showed that P6 could repress the production of secondary siRNAs and inhibit systemic green fluorescent protein RNA silencing but did not interfere with local RNA silencing in N. benthamiana plants or in rice protoplasts. Intriguingly, P6 and RDR6 had overlapping subcellular localization and P6 bound both rice and Arabidopsis RDR6 in vivo. Furthermore, transgenic rice plants expressing P6 showed enhanced susceptibility to infection by Rice stripe virus. Hence, we propose that P6 is part of the RYSV's counter-defense machinery against the plant RNA silencing system and plays a role mainly in affecting RDR6-mediated secondary siRNA synthesis. Our work provides a new perspective on how a plant-infecting nucleorhabdovirus may counteract host RNA silencing-mediated antiviral defense.


Asunto(s)
Proteínas de Plantas/metabolismo , ARN Interferente Pequeño/metabolismo , Rhabdoviridae/metabolismo , Proteínas Virales/metabolismo , Animales , Citoplasma/metabolismo , Regulación de la Expresión Génica de las Plantas , Oryza/metabolismo , Enfermedades de las Plantas/virología , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente , Plásmidos , Potexvirus/patogenicidad , Interferencia de ARN , Tabaco/virología , Proteínas Virales/clasificación , Proteínas Virales/genética , Virulencia
16.
Angew Chem Int Ed Engl ; 52(9): 2555-8, 2013 Feb 25.
Artículo en Inglés | MEDLINE | ID: mdl-23364901

RESUMEN

Efficient: A copper-catalyzed enantioselective intramolecular insertion of carbenoids into phenolic O-H bonds has been developed. This method can be used for the synthesis of the title compounds in high yields and excellent enantioselectivities under mild and neutral conditions. NaBAr(F)=sodium tetrakis[3,5-bis(trifluoromethyl)phenyl]borate.

17.
Plant J ; 69(1): 104-15, 2012 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-21880078

RESUMEN

Cucumber mosaic virus suppressor 2b (CMV2b) is a nuclear viral suppressor that interferes with local and systemic silencing and inhibits AGO1 slicer activity. CMV2b-mediated transgene hypomethylation and its localization in Cajal bodies suggests a role of CMV2b in RNA-directed DNA methylation (RdDM). However, its direct involvement in RdDM, or its binding with small RNAs (sRNAs) in vivo is not yet established. Here, we show that CMV2b binds both microRNAs (miRNAs) and small interfering RNAs (siRNAs) in vivo. sRNA sequencing data from the CMV2b immunocomplex revealed its preferential binding with 24-nt repeat-associated siRNAs. We provide evidence that CMV2b also has direct interaction with the AGO4 protein by recognizing its PAZ and PIWI domains. Subsequent analysis of AGO4 functions revealed that CMV2b reduced AGO4 slicer activity and the methylation of several loci, accompanied by the augmented accumulation of 24-nt siRNAs in Arabidopsis inflorescences. Intriguingly, CMV2b also regulated an AGO4-related epiallele independently of its catalytic potential, which further reinforces the repressive effects of CMV2b on AGO4 activity. Collectively, our results demonstrate that CMV2b can counteract AGO4-related functions. We propose that by adopting novel counter-host defense strategies against AGO1 and AGO4 proteins, CMV creates a favorable cellular niche for its proliferation.


Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/virología , Proteínas Argonauta/metabolismo , Cucumovirus/fisiología , Interacciones Huésped-Patógeno , MicroARNs/metabolismo , ARN Interferente Pequeño/metabolismo , Proteínas Virales/metabolismo , Adenosina/metabolismo , Arabidopsis/metabolismo , Metilación de ADN , Plantas Modificadas Genéticamente/virología , Estructura Terciaria de Proteína , Secuencias Repetitivas de Ácidos Nucleicos , Proteínas Virales/genética
18.
J Am Chem Soc ; 132(46): 16374-6, 2010 Nov 24.
Artículo en Inglés | MEDLINE | ID: mdl-21033664

RESUMEN

A copper-catalyzed asymmetric intramolecular O-H insertion of ω-hydroxy-α-diazoesters has been accomplished by using chiral spiro bisoxazoline ligands. This highly enantioselective intramolecular O-H insertion reaction provides an efficient approach to a variety of synthetically important chiral 2-carboxy cyclic ethers with different ring sizes as well as substitution patterns.


Asunto(s)
Cobre/química , Éteres Cíclicos/química , Catálisis , Hidrógeno/química , Estructura Molecular , Oxazoles/química , Oxígeno/química , Estereoisomerismo
19.
Sheng Wu Gong Cheng Xue Bao ; 25(8): 1130-7, 2009 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-19938448

RESUMEN

Newborn ovary homeobox gene (NOBOX) is an oocyte-specific homeobox gene that plays a critical role in early folliculogenesis and represents a candidate gene for nonsyndromic ovarian failure. We used in silico approach in combination with rapid amplification of cDNA ends (RACE) to clone the full-length cDNA of NOBOX (GenBank Accession No. FJ587509) from porcine oocytes. It contains 1768 bp nucleotides, with an open reading frame (ORF) of 1419 bp. The putative porcine NOBOX gene encodes 472 amino acids with the molecular weight of 51.08 kD and pI of 5.73. Bioinformatics prediction indicates that this protein contains a cd00086 homeodomain. Real-time PCR analysis showed that the NOBOX gene is expressed in various tissues, oocytes and embryos cells (4-cell, 8-cell, morula and blastocyst) at different expression levels. The expression levels of this gene in heart, kidney and oocytes are higher than that in other tissues, which suggested that the NOBOX protein might play an important role in those tissues. The expression of NOBOX in developmental stages is higher than that in GV-stage oocytes, which suggested that the expression of pNOBOX was enhanced in developmental stages.


Asunto(s)
Proteínas de Homeodominio/genética , Oocitos/metabolismo , Porcinos/genética , Secuencia de Aminoácidos , Animales , Clonación Molecular , ADN Complementario/genética , Desarrollo Embrionario/genética , Femenino , Proteínas de Homeodominio/metabolismo , Masculino , Datos de Secuencia Molecular , Porcinos/metabolismo
20.
Artículo en Chino | MEDLINE | ID: mdl-19514577

RESUMEN

OBJECTIVE: To investigate the operative method of trabecular metal rod implant for the treatment of the early adult avascular necrosis of the femoral head (ANFH) and its clinical efficacy. METHODS: From March 2005 to January 2007, 28 patients of ANFH were treated by trabecular metal rod implant, including 18 males and 10 females aged 18- 56-years-old (average 30.6-years-old). The ANFH was induced by trauma in 7 cases, steroid in 10 cases, alcohol in 5 cases and unknown reason in 6 cases. There were 22 cases of unilateral ANFH and 6 of bilateral ANFH, involving 18 left hips and 16 right hips. According to Association Research Circulation Osseous (ARCO) international classification of osteonecrosis launched in 1997, 16 cases (16 hips) were classified as stage I, 12 cases (15 hips) as stage II, 1 case (1 hip) as stage III and 2 cases (2 hips) as stage IV. Harris score was 50.3 +/- 2.2 preoperatively. The course of disease was 12-24 months (average 16 months). RESULTS: All wounds healed by first intention, and no postoperative complication occurred. All the cases were followed up for 18-36 months (average 26 months). The pain and functional limitation of patients were improved significantly. The Harris score was 85.7 +/- 2.4 18 months after operation, showing a significant difference when compared with preoperative score (P < 0.05). Twenty-three cases were graded as excellent, 3 as good and 2 as fair, with the excellent and good rate of 92.86%. CONCLUSION: Trabecular metal rod implant is an effective method for the treatment of early ANFH and can minimize the occurrence of complications, but the follow-up observation of its long-term therapeutic effect is still needed.


Asunto(s)
Artroplastia de Reemplazo de Cadera/instrumentación , Artroplastia de Reemplazo de Cadera/métodos , Necrosis de la Cabeza Femoral/cirugía , Adolescente , Adulto , Femenino , Humanos , Fijadores Internos , Masculino , Metales , Persona de Mediana Edad , Resultado del Tratamiento , Adulto Joven
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...