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1.
J Clin Exp Dent ; 12(12): e1196-e1200, 2020 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-33282142

RESUMEN

Thrombocytopenia-absent radius (TAR) syndrome is a congenital malformation in which affected individuals present reductions in the number of platelets, hypoplasia, or absence of radial bone unilaterally or bilaterally. Hematologic, skeletal, cardiac (particularly tetralogy of Fallot and septal-atrial defects), and gastrointestinal anomalies are most commonly associated with TAR syndrome. Skeletal changes result in a higher risk of dental and craniofacial trauma in patients with the syndrome. Thus, it is important for the dentist to be aware of the characteristics of TAR syndrome and its clinical management for better care of these patients. The objective of this study is to describe a case report of a 26-year-old patient with TAR syndrome with a history of trauma and root fracture of tooth 11 and alveolar bone ridge. During anamnesis, root fractures requiring the extraction of the 11 tooth, alveolar bone ridge fracture in the adjacent region, and dental trauma were observed. A hematological evaluation and blood and radiological examinations were performed. Osseointegrated implant was performed using the guided surgery and flapless technique, as well as prosthetic rehabilitation in the affected region. This report discusses the importance of careful planning, such as the use of incisions and conservative surgery, techniques for alveolar ridge preservation, gingival manipulation, and prosthesis confection. The patient was attended by a hematologist throughout the treatment. Key words:TAR syndrome, absent radii and thrombocytopenia, dental implants, oral surgery.

2.
Rev. Odontol. Araçatuba (Impr.) ; 41(3): 33-39, set./dez. 2020. ilus
Artículo en Portugués | LILACS, BBO - Odontología | ID: biblio-1121742

RESUMEN

Este relato de caso clínico tem como objetivo descrever a utilização de osso alógeno na reconstrução de maxila atrófica para posterior reabilitação com prótese fixa metalocerâmica sobre implantes, fazer uma análise histológica do tecido ósseo enxertado e descrever o acompanhamento clínico e radiográfico por 8 anos. Uma paciente de 54 anos, com edentulismo parcial e atrofia moderada-a-severa na maxila, apresentou-se para tratamento demonstrando muito interesse em receber prótese sobre implantes. Após exame clínico e radiográfico, foi realizada montagem dos modelos de estudo em ASA para enceramento diagnóstico e obtenção de um guia multifuncional (tomográfico/ cirúrgico). A tomografia indicou a necessidade de reconstrução óssea maxilar. Procedeuse então à aposição de blocos de osso alógeno para aumento horizontal nas regiões anterior e posterior da maxila. Um dos blocos foi triturado e utilizado para levantamento do assoalho do seio maxilar no lado esquerdo. Dez meses depois, uma nova tomografia foi solicitada, utilizando o mesmo guia inicial, e os implantes instalados, utilizando o guia multifuncional como guia cirúrgico. Neste momento, material ósseo foi coletado na interface osso enxertado/osso nativo com uma broca trefina. Os resultados histológicos demonstraram viabilidade das células ósseas no enxerto, além da presença de vasos sanguíneos. Após o tempo necessário para osseointegração, procedeu-se com tratamento protético. Nenhuma complicação foi relatada até oito anos de controle. A sequência de tratamento proposta forneceu bons resultados estéticos e funcionais. Concluiu-se, então, que o emprego de osso alógeno é uma alternativa viável para a reconstrução de rebordos alveolares severamente reabsorvidos(AU)


This clinical case report aims to describe the use of allogeneic bone in the atrophic maxilla reconstruction for subsequent rehabilitation with a fixed metal-ceramic prosthesis on implants; to perform a histological analysis of the grafted bone tissue; and to describe the clinical and radiographic monitoring for 8 years. A 54-year-old patient, with partial edentulism and moderate-to-severe atrophy in the maxilla showed great interest in receiving implant prostheses. After c linical and radiographic examination, the ASA study models were assembled for diagnostic waxing and a multifunctional guide (tomographic / surgical) was obtained. Tomography indicated the need for maxillary bone reconstruction. Allogeneic bone blocks were then placed for horizontal enlargement in the anterior and posterior regions of the maxilla. One of the blocks was crushed and used to lift the floor of the maxillary sinus on the left side. Ten months later, a new tomography was requested, using the same initial guide, and the implants installed, using the multifunctional guide as a surgical guide. At this time, bone material was collected at the grafted bone / native bone interface with a trephine drill. Histological results demonstrated viability of bone cells in the graft, besides the presence of blood vessels. After the necessary time for osseointegration, a prosthetic treatment was performed. No complications were reported up to eight years of control. The propose treatment sequence provided good aesthetic and functional results. It was concluded, then, that the use of allogeneic bone is a viable alternative for the reconstruction of severely reabsorbed alveolar edges(AU)


Asunto(s)
Trasplante Óseo , Prótesis Dental de Soporte Implantado , Diseño de Prótesis Dental , Elevación del Piso del Seno Maxilar
3.
J Clin Exp Dent ; 12(10): e999-e1004, 2020 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-33154803

RESUMEN

Metastasis to the oral cavity are rare, representing only 1% of all oral malignancies, and originate from various sites such as the breast, prostate, lung and kidney. Clinically, they can simulate reactive and inflammatory lesions common in the oral cavity, and the clinical and microscopic diagnosis of these metastasis is a challenge. In this article, we report two new cases of esophageal and lung metastasis to oral tissues, highlighting their clinical characteristics and the process of diagnostic elucidation. We emphasize the importance for clinicians to consider the possibility of metastatic lesions in the oral cavity in patients previously diagnosed with malignant lesions in distant tissues and organs. Key words:Diagnosis, esophageal squamous cell carcinoma, adenocarcinoma of lung, oral cavity, metastasis.

5.
RGO (Porto Alegre) ; 68: e20200002, 2020. graf
Artículo en Inglés | LILACS-Express | LILACS | ID: biblio-1091887

RESUMEN

ABSTRACT Jaw metastasis can be mistaken for inflammatory or infectious diseases. Then, they should be considered in the differential diagnosis of unknown jaw lesions. Study reported here involved metastasis of breast cancer in the mandible of a 45-year-old woman. The most important differential diagnostic was a reactive lesion in an unusual periapical location associated with a nonvital tooth. However, given patient's medical history and because paresthesia and pain were observed a few days after pulpectomy, metastasis of breast cancer could not be ruled out. When bone scintigraphy suggested the metastasis of a malignant bone tumor, incisional biopsy was performed. Histopathologic examination and immunohistochemical reaction confirmed the diagnosis of metastasis of breastcancer, and chemotherapy was thus performed as well. Unfortunately, patient died 2 years after diagnosis. Dentists as well as general physicians should therefore consider presence of oral metastasis in cases involving atypical symptoms, especially in patients with known malignant disease.


RESUMO As metástases mandibulares podem ser confundidas com doenças inflamatórias ou infecciosas. Sendo assim, elas devem ser consideradas no diagnóstico diferencial de lesões desconhecidas na mandíbula. Esse relato de caso envolveu metástase de câncer de mama na mandíbula de uma mulher de 45 anos. O diagnóstico diferencial mais importante foi uma lesão reacional, em uma localização periapical incomum, associada a um dente não vital. No entanto, dado o histórico médico da paciente e uma vez que parestesia e dor foram observadas alguns dias após a realização da pulpectomia, a metástase de câncer de mama não foi descartada. Quando a cintilografia óssea sugeriu a metástase de um tumor ósseo maligno, uma biópsia incisional foi realizada. O exame histopatológico e a reação imunohistoquímica confirmaram o diagnóstico de metástase do câncer de mama, e a quimioterapia foi então realizada. Infelizmente, a paciente morreu 2 anos após o diagnóstico. Portanto, dentistas e médicos generalistas devem considerar a presença demetástases oral em casos que envolvam sintomas atípicos, principalmente em pacientes com doença maligna conhecida.

6.
Artículo en Inglés | MEDLINE | ID: mdl-33610538

RESUMEN

OBJECTIVE: The objective of this study was to describe the clinicopathological, molecular, and prognostic features of oral/oropharyngeal diffuse large B-cell lymphoma (DLBCL) and high-grade B-cell lymphoma. STUDY DESIGN: All cases were retrieved from 7 Brazilian institutions. Immunohistochemical reactions were performed to confirm the diagnoses and to categorize the tumors. In situ hybridization was used to detect Epstein-Barr virus (EBV) and fluorescence in situ hybridization was used to identify gene rearrangements. RESULTS: Most cases involved the oral cavity (76.8%). Males and females, with a mean age of 60 years, were evenly affected. Tumors mostly presented as painful swellings. Forty cases represented germinal center B-cell type (58%). Five cases presented double-hit translocation and 3 harbored rearrangement for MYC/BCL2/BCL6. EBV was detected in 3 cases (4.3%). The 5-year overall survival was 44.4%. Female sex, presence of pain and ulcer, microscopic "starry sky pattern" and necrosis, co-expression of c-Myc/Bcl2, and translocation of MYC were associated with a lower survival in univariate analysis (P = .05, P = .01, P = .01, P = .03, P = .05, P = .006, P = .05, respectively). CONCLUSION: Patients affected by oral/oropharyngeal DLBCL have a low survival rate. High-grade B-cell lymphoma (17.7%) and EBV-positive DLBCL, not otherwise specified (4.3%) account for a small number of cases.

7.
J Investig Clin Dent ; 10(4): e12472, 2019 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-31560456

RESUMEN

AIM: To evaluate biological behavior of human peripheral mononuclear cells (PBMC) in contact with porous tantalum (PT) and Porphyromonas gingivalis (Pg). METHODS: Pg was incubated for 8 hours. The groups formed were: PBMC (control), PBMC + PT, PBMC + Pg and PBMC + PT + Pg. Cell viability was evaluated using MTT assay. The morphology and adhesion of PBMC to PT was evaluated using scanning electron microscopy. Expression of interleukin (IL)-10, transforming growth factor (TGF)-ß, matrix metallopeptidase (MMP)-9 and receptor activator of nuclear factor-κΒ ligand (RANKL) was determined by enzyme-linked immunosorbent assay. RESULTS: MTT assay revealed that PT did not interfere in the mitochondrial activity of PBMC (P > .05). Scanning electron microscopy showed the adherence of PBMC to PT. IL-10 levels in PBMC + PT were similar to PBMC and lower than PBMC + Pg. TGF-ß levels in PBMC + PT were higher than PBMC and PBMC + Pg. MMP-9 levels in PBMC + PT were similar to PBMC and lower than PBMC + Pg and PBMC + PT + Pg. RANKL levels in PBMC + PT were lower than in PBMC. CONCLUSION: PT did not affect PBMC viability, allowed cell adhesion, reduced expression of RANKL and enhanced TGF-ß in comparison with the control group.


Asunto(s)
Porphyromonas gingivalis , Tantalio , Humanos , Leucocitos , Leucocitos Mononucleares , Porosidad
8.
Bone ; 128: 115041, 2019 11.
Artículo en Inglés | MEDLINE | ID: mdl-31442676

RESUMEN

The renin-angiotensin system (RAS), aside its classical hormonal properties, has been implicated in the pathogenesis of inflammatory disorders. The angiotensin converting enzyme 2/angiotensin-(1-7)/Mas Receptor (ACE2/Ang-(1-7)/MasR) axis owns anti-inflammatory properties and was recently associated with bone remodeling in osteoporosis. Thus, the aim of this study was to characterize the presence and effects of the ACE2/Ang-(1-7)/MasR axis in osteoblasts and osteoclasts in vitro and in vivo. ACE2 and MasR were detected by qPCR and western blotting in primary osteoblast and osteoclast cell cultures. Cells were incubated with different concentrations of Ang-(1-7), diminazene aceturate (DIZE - an ACE2 activator), A-779 (MasR antagonist) and/or LPS in order to evaluate osteoblast alkaline phosphatase and mineralized matrix, osteoclast differentiation and cytokine expression, and mRNA levels of osteoblasts and osteoclasts markers. An experimental model of alveolar bone resorption triggered by dysbiosis in rats was used to evaluate bone remodeling in vivo. Rats were treated with Ang-(1-7), DIZE and/or A-779 and periodontal samples were collected for immunohistochemistry, morphometric analysis, osteoblast and osteoclast count and cytokine evaluation. Human gingival samples from healthy and periodontitis patients were also evaluated for detection of ACE2 and MasR expression. Osteoblasts and osteoclasts expressed ACE2 and MasR in vitro and in vivo. LPS stimulation or alveolar bone loss induction reduced ACE2 expression. Treatment of bone cells with Ang-(1-7) or DIZE stimulated osteoblast ALP, matrix synthesis, upregulated osterix, osteocalcin and collagen type 1 transcription, reduced IL-6 expression, and decreased osteoclast differentiation, RANK and IL-1ß mRNA transcripts, and IL-6 and IL-1ß levels, in a MasR-dependent manner. In vivo, Ang-(1-7) and DIZE decreased alveolar bone loss through improvement of osteoblast/osteoclast ratio. A-779 reversed such phenotype. ACE2/Ang-(1-7)/MasR axis activation reduced IL-6 expression, but not IL-1ß. ACE2 and MasR were also detected in human gingival samples, with higher expression in the healthy than in the inflamed tissues. These findings show that the ACE2/Ang-(1-7)/MasR is an active player in alveolar bone remodeling.


Asunto(s)
Angiotensina I/metabolismo , Remodelación Ósea/fisiología , Fragmentos de Péptidos/metabolismo , Peptidil-Dipeptidasa A/metabolismo , Fosfatasa Alcalina/genética , Fosfatasa Alcalina/metabolismo , Proceso Alveolar/metabolismo , Angiotensina I/genética , Animales , Animales Recién Nacidos , Western Blotting , Remodelación Ósea/genética , Supervivencia Celular/genética , Supervivencia Celular/fisiología , Células Cultivadas , Ensayo de Inmunoadsorción Enzimática , Humanos , Inmunohistoquímica , Técnicas In Vitro , Masculino , Osteoblastos/citología , Osteoblastos/metabolismo , Osteoclastos/citología , Osteoclastos/metabolismo , Fragmentos de Péptidos/genética , Peptidil-Dipeptidasa A/genética , Proteínas Proto-Oncogénicas , Ratas , Ratas Wistar , Reacción en Cadena en Tiempo Real de la Polimerasa , Receptor de Angiotensina Tipo 2/genética , Receptor de Angiotensina Tipo 2/metabolismo , Receptores Acoplados a Proteínas G , Sistema Renina-Angiotensina/genética , Sistema Renina-Angiotensina/fisiología
9.
Head Neck Pathol ; 13(4): 535-542, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-30430416

RESUMEN

Tumor budding is a prognostic marker for oral squamous cell carcinoma (OSCC) characterized by the presence of isolated or small clusters of neoplastic cells at the tumor invasive front. Aldehyde dehydrogenase-1 (ALDH1) is associated with tumorigenesis, linked to treatment resistance and shown to identify cancer stem cells (CSC)-like cells. This study aimed to evaluate the expression of ALDH1 and its association with tumor budding in OSCC. Immunohistochemistry was employed in 163 OSCC samples to identify pancytokeratin (AE1/AE3) and ALDH1. While pancytokeratin (AE1/AE3) identified squamous tumor buds, the CSC-like cells were identified using ALDH1. A Chi square test was used to evaluate association between ALDH1 expression and tumor budding, while McNemar's test was used to identify differences in ALDH1 expression between the budding area and the area outside the budding. A positive expression of ALDH1 was observed in 47.24% of the samples and in 70% of anatomic locations affected. No association was observed between ALDH1 expression and tumor budding (p > 0.05). In tumors with high-intensity tumor budding, ALDH1 expression was higher in the budding area than in the area outside the budding (p < 0.05). The finding that tumor bud cells in OSCC show phenotypic characteristics of CSC-like cells reinforces the relevance of tumor budding in determining the biological behavior of this malignant neoplasm. Moreover, the presence of CSC-like cells in nearly half of evaluated samples of OSCC and in most of the affected anatomic locations is in accordance with the CSC model of oral carcinogenesis.


Asunto(s)
/biosíntesis , Biomarcadores de Tumor/análisis , Neoplasias de la Boca/patología , Células Madre Neoplásicas/patología , Carcinoma de Células Escamosas de Cabeza y Cuello/patología , Adulto , Anciano , Femenino , Humanos , Inmunohistoquímica , Masculino , Persona de Mediana Edad
10.
Braz. j. oral sci ; 18: e191445, jan.-dez. 2019. ilus
Artículo en Inglés | LILACS, BBO - Odontología | ID: biblio-1094900

RESUMEN

Aim: The objective was to compare the density and degranulation of mast cells on specimens obtained from individuals diagnosed with gingivitis or chronic periodontitis who were either non-HIV-infected or HIV-infected patients treated with highly active antiretroviral therapy (HAART). Methods: Gingival samples were taken from 16 non-HIV-infected individuals and 17 HIV-infected individuals diagnosed with gingivitis and chronic periodontitis. The samples were processed and tained with 0.3 percent o-toluidine blue. Densities (cells/mm²) and percentages of intact and degranulated mast cells were obtained. Results: No statistically significant differences were observed in the mast cell density and the percentage of degranulated mast cells between non-HIV-infected and HIV-infected individuals diagnosed with gingivitis and chronic periodontitis. Mononuclear inflammatory infiltrate was weakly correlated with the percentage of mast cells degranulated for both groups. Conclusions: There are no differences of the density and degranulation of mast cells in gingival tissue between non-HIV-infected and HIV-infected patients undergoing HAART, both groups with diagnosis of gingivitis or chronic periodontitis. This may be a result of the recovery of the immunologic system by HAART treatment.


Asunto(s)
Humanos , Masculino , Femenino , Enfermedades Periodontales , VIH , Periodontitis Crónica , Gingivitis , Mastocitos , Mucosa Bucal
11.
Arch Oral Biol ; 95: 51-57, 2018 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-30056280

RESUMEN

OBJECTIVE: To compare the densities of CD1a + immature and CD83+ mature dendritic cells, and inflammatory infiltrate cells between smokers and non-smokers with oral leukoplakia. Parameters associated with malignant transformation were also evaluated. DESIGN: 21 smokers and 23 non-smokers diagnosed with oral leukoplakia were obtained. Densities of inflammatory infiltrate cells were calculated in H&E sections. Immunohistochemistry using anti-CD1a and anti-CD83 was performed and densities were calculated. Comparisons and statistical analyses were performed among the groups and parameters as gender, lesion size, site, and presence of cell dysplasia were analyzed. RESULTS: A lower density of CD83+ cells was observed in smokers compared to non-smokers (P < 0.05). For samples of smokers, a lower density of CD1a + cells, CD83+ cells, and inflammatory infiltrate cells was observed in samples with <10 mm compared to samples ≥10 mm of diameter (P < 0.05), and a lower density of CD83+ cells was also observed between samples without dysplasia compared to samples with dysplasia (P < 0.05). CONCLUSION: In oral leukoplakia samples, dendritic cell density decreases in the presence of smoking habit, and increases in larger lesions and with epithelial dysplasia. Smoking habit is an external factor that contribute to alteration of the anti-tumoral immune defense system in lesions of oral leucoplakia, reinforcing that smoking elimination is important to control the development of this disease.


Asunto(s)
Células Dendríticas/inmunología , Células Epiteliales/inmunología , Leucoplasia Bucal/inmunología , Fumar/inmunología , Brasil , Células Dendríticas/patología , Células Epiteliales/patología , Femenino , Humanos , Técnicas para Inmunoenzimas , Técnicas In Vitro , Leucoplasia Bucal/patología , Masculino , Persona de Mediana Edad
12.
Artículo en Inglés | MEDLINE | ID: mdl-27086000

RESUMEN

OBJECTIVE: To evaluate and compare the number of CD1a+ dendritic cells (DCs) in oral lichen planus (OLP), amalgam lichenoid reaction (ALR), and normal oral mucosa (NOM). STUDY DESIGN: Fourteen cases of OLP and ALR, diagnosed both clinically and histologically, were obtained from an archive of an oral pathology service. Seven specimens of NOM were collected and served as controls. Immunohistochemistry was used to identify CD1a+ DCs. Densities were calculated using specimens of epithelium and lamina propria. For OLP and ALR, specimens of lamina propria included cells from inflammatory infiltrate (region A), and from below inflammatory infiltrate (region B). Comparisons and statistical analyses were performed among OLP, ALR, and NOM. RESULTS: A higher density of CD1a+ DCs was observed in OLP compared to ALR in region A (P < .05) and regions A+B (P < .05). A higher density of CD1a+ DCs was also observed in OLP compared with NOM in the regions A+B (P < .05). Statistically significant differences were not observed for analyses involving epithelial tissue. CONCLUSIONS: The observation that there is a lower quantity of DCs in ALR specimens compared with specimens from individuals with OLP suggests that the role for DCs in the 2 diseases may be different. The difference in cell density could be used in the differential diagnosis of these 2 diseases, but the overlapping intervals in the statistical analysis may limit the application of this approach in surgical practice.


Asunto(s)
Antígenos CD1/análisis , Células Dendríticas/patología , Amalgama Dental/efectos adversos , Liquen Plano Oral/patología , Adulto , Anciano , Recuento de Células , Femenino , Humanos , Inmunohistoquímica , Masculino , Persona de Mediana Edad , Mucosa Bucal/patología
13.
Immunobiology ; 219(12): 909-15, 2014 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-25172546

RESUMEN

AIM: Evaluate the effects of smoking on dendritic cells (DCs), cytokines, clinical periodontal parameters, and number of teeth in samples of human chronic periodontitis (CP). MATERIAL AND METHODS: Gingival samples were obtained from 24 smokers and 21 non-smokers with CP. Periodontal examination was carried out. Immunohistochemical staining was performed to identify Factor XIIIa+ immature, CD1a+ immature, and CD83+ mature DCs. The inflammatory infiltrate was counted, and IL-2, IL-10, IL-4, IL-6, IFN-γ, TNF-α, and IL-17A were measured using the cytometric bead array (CBA). Inflammatory infiltrate, DCs, cytokines, classification of CP, clinical periodontal parameters, number of teeth, smoking habit in years (SH/years), and number of cigarettes smoked per day (C/day) were correlated and compared. RESULTS: CD83+ mature DCs decreased in the smokers group. Negative correlations could be observed between the number of C/day with levels of IL-17A and number of teeth. Correlations between smoking, periodontal disease status, and other cytokines were not observed. CONCLUSIONS: Smoking decreases mature DCs in chronic periodontitis. Moreover, a dose-dependent relation can be observed between C/day and number of teeth and levels of IL17A observed. Smokers show a different modulation of the CP immune response.


Asunto(s)
Periodontitis Crónica/etiología , Inmunidad , Fumar/efectos adversos , Adulto , Anciano , Recuento de Células , Periodontitis Crónica/metabolismo , Periodontitis Crónica/patología , Citocinas/metabolismo , Células Dendríticas/inmunología , Células Dendríticas/metabolismo , Femenino , Humanos , Masculino , Persona de Mediana Edad , Fenotipo , Adulto Joven
14.
Immunobiology ; 219(8): 633-6, 2014 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-24780137

RESUMEN

AIM: Evaluate the effects of smoking on chemokines of the human chronic periodontitis (CP). MATERIALS AND METHODS: Gingival samples were obtained from 23 smokers (S) and 20 non-smokers (NS) diagnosed with CP. Periodontal examination was performed. The CCL2, CCL3, CCL5, CCL19, CCL20, and CXCL8 chemokine levels were measured in gingival tissues using enzyme-linked immunosorbent assay. Chemokines were compared between S and NS, and were correlated with the number of cigarettes per day (C/day) and time of the smoking habit in years (SH/years). RESULTS: CCL3 and CXCL8 of S were significantly smaller than that found in NS subjects, whereas the CCL5 levels increased in the S group. Negative correlations could be observed between CCL19 levels and SH/year. CONCLUSION: Smoking suppresses the immune response which may contribute to an increased susceptibility to periodontal disease in smokers.


Asunto(s)
Quimiocinas/metabolismo , Periodontitis Crónica/inmunología , Encía/inmunología , Adolescente , Adulto , Anciano , Susceptibilidad a Enfermedades/inmunología , Regulación hacia Abajo , Femenino , Humanos , Inmunosupresión , Masculino , Persona de Mediana Edad , Fumar/efectos adversos , Adulto Joven
15.
J Periodontol ; 85(10): 1416-23, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-24605873

RESUMEN

BACKGROUND: The purpose of this study was to evaluate the relationship between chemokines and dendritic cells (DCs) in human chronic periodontitis (CP). METHODS: Gingival samples were obtained from 23 individuals with CP, and six samples of normal mucosa (NM) overlapping the third molar were used to control for the chemokine levels. Periodontal examination was conducted. Immunohistochemistry was performed for Factor XIIIa(+) and cluster of differentiation (CD)1a(+) immature DCs and CD83(+) mature DCs. Levels of the CC chemokine ligand (CCL)2, CCL3, CCL5, CCL19, CCL20, and CXC chemokine ligand (CXCL)8 were measured in gingival tissues using enzyme-linked immunosorbent assay. Inflammatory infiltrate, DCs, chemokines, classification of human CP, and clinical parameters were correlated and compared. RESULTS: The expression of CCL2 and CCL20 was positively correlated with increased densities of CD1a(+) DCs. CCL3 and CXCL8 were positively related to the clinical attachment level. CCL3, CCL5, CCL19, and CXCL8 levels increased in the gingival samples of patients with CP compared with NM, whereas CCL20 levels increased in advanced CP compared with mild-moderate CP. CONCLUSIONS: More CD1a(+) immature DCs are related to CCL2 and CCL20. CCL3 and CXCL8 chemokines are related to a greater severity of human CP.


Asunto(s)
Quimiocinas CC/inmunología , Periodontitis Crónica/inmunología , Células Dendríticas/inmunología , Adulto , Anciano , Antígenos CD/inmunología , Antígenos CD1/inmunología , Recuento de Células , Quimiocina CCL19/inmunología , Quimiocina CCL2/inmunología , Quimiocina CCL20/inmunología , Quimiocina CCL3/inmunología , Quimiocina CCL5/inmunología , Periodontitis Crónica/clasificación , Periodontitis Crónica/patología , Factor XIIIa/análisis , Femenino , Encía/inmunología , Hemorragia Gingival/inmunología , Humanos , Inmunoglobulinas/inmunología , Interleucina-8/inmunología , Masculino , Glicoproteínas de Membrana/inmunología , Persona de Mediana Edad , Mucosa Bucal/inmunología , Pérdida de la Inserción Periodontal/inmunología , Bolsa Periodontal/inmunología , Adulto Joven
16.
PLoS One ; 9(3): e91636, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24670840

RESUMEN

There are a limited number of studies correlating the different stages of dendritic cells (DC) maturation with cytokines in individuals presented chronic periodontitis (CP). The aim of the study was to evaluate the correlation among the expression of IL-2, IL-10, IL-4, IL-6, IFN-γ, TNF-α, and IL-17A with the presence of DC and mild-moderate or advanced CP. Gingival samples were obtained from 24 individuals with CP and six samples of normal mucosa (NM) overlapping third molar for controls of the levels of cytokines. Periodontal examination was performed. Immunohistochemical staining was carried out, revealing CD1a+ immature, Fator XIIIa+ immature, and CD83+ mature DCs. The inflammatory infiltrate was counted, and the cytokines were measured by flow cytometry. Densities of DCs and inflammatory infiltrate, cytokines, subtypes of CP, and clinical periodontal parameters were correlated and compared. IL-6 expression was correlated positively with the increased numbers of CD1a+ immature DCs. Levels of IL-2, TNF-α, IFN-γ, IL-10, and IL-17A were increased when compared with NM. The percentage of sites with clinical attachment level (CAL)>3 were positively correlated with densities of inflammatory infiltrate and negatively correlated with densities of immature DCs. IL-6 can contribute to the increase of the immature DCs in the CP. Higher levels of IL-2, TNF-α, IFN-γ, IL-10, and IL-17A cytokines were observed in CP. Higher densities of inflammatory infiltrate as well as lower densities of immature DCs can result in a more severe degree of human CP.


Asunto(s)
Periodontitis Crónica/metabolismo , Citocinas/metabolismo , Células Dendríticas/metabolismo , Mediadores de Inflamación/metabolismo , Células TH1/metabolismo , Células Th17/metabolismo , Células Th2/metabolismo , Adulto , Recuento de Células , Diferenciación Celular , Periodontitis Crónica/patología , Estudios Transversales , Femenino , Encía/patología , Humanos , Inflamación/patología , Masculino , Membrana Mucosa/patología
17.
J Clin Exp Dent ; 6(5): e588-91, 2014 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-25674331

RESUMEN

Odontogenic fibroma (OF) is a rare benign odontogenic neoplasm that is most commonly found in the mandibular/premolar region of female patients in the second to fourth decades of life. Well-defined radiolucent lesions that may induce root resorption are normally observed. Rare variants of OF have been described in the prior literature, including references to: 1) giant cell lesions, 2) amyloid-like protein deposition, and 3) ossifying variants. Immunohistochemistry can contribute to understanding the biological behavior and the pathogenesis of OF. Therefore, this case report aimed to describe a new case of ossifying OF and discuss the histopathology and immunohistochemical features. Key words:Odontogenic fibroma, jaw tumors, ossifying variant.

18.
J Oral Pathol Med ; 43(3): 177-82, 2014 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-24125466

RESUMEN

BACKGROUND: Odontogenic tumors exhibited variable biologica behaviors. Metallothionein (MT) is correlated with the cellular homeostasis of essential metals, cellular differentiation, and proliferation. The core goals of this study are (i) to report and to compare MT expression among benign epithelial odontogenic tumors; (ii) to correlate MT with cellular proliferation index; and (iii) to evaluate the influence of the inflammatory infiltrate on MT expression. MATERIALS AND METHODS: Ten cases of solid ameloblastomas (SABs), 4 squamous odontogenic tumors (SOTs), 5 adenomatoid odontogenic tumors (AOTs), and 3 calcifying epithelial odontogenic tumors (CEOTs) were subjected to immunohistochemical to anti-MT, anti-Ki-67, and anti-PCNA. Statistical analysis was performed using BioEstat(®) 4.0. RESULTS: Metallothionein staining was found to be the highest in the SABs (93.1%), followed by SOTs (52.9%), AOTs (38.4%), and CEOTs (0%). MT staining exhibited statistically significant differences between the SABs and the SOTs (P = 0.0047) and the AOTs (P = 0.0022). A weak-to-strong positive correlation between IMT and IK or IP was observed in SABs and SOTs, whereas a strong negative correlation was observed in AOTs. No differences in IMT, IK, and IP were observed between inflammation groups A and B. CONCLUSIONS: The increased MT expression observed in the SABs might be correlated with clinical behavior (local invasiveness and high rate of recurrence). In the SABs and SOTs, MT plays a role in the stimulation of cellular proliferation. In contrast, MT can inhibit cellular proliferation in the AOT. The IMT, IK, and IP are not affected by inflammation.


Asunto(s)
Metalotioneína/análisis , Tumores Odontogénicos/química , Ameloblastoma/química , Ameloblastoma/patología , Proliferación Celular , Tejido Conectivo/patología , Células Epiteliales/química , Células Epiteliales/patología , Humanos , Inmunohistoquímica , Antígeno Ki-67/análisis , Linfocitos/patología , Invasividad Neoplásica , Recurrencia Local de Neoplasia/patología , Tumor Odontogénico Escamoso/química , Tumor Odontogénico Escamoso/patología , Tumores Odontogénicos/patología , Células Plasmáticas/patología , Antígeno Nuclear de Célula en Proliferación/análisis , Neoplasias Cutáneas/química , Neoplasias Cutáneas/patología
19.
Belo Horizonte; s.n; 2014. 159 p. ilus, tab.
Tesis en Portugués | LILACS, BBO - Odontología | ID: lil-715980

RESUMEN

A periodontite crônica (PC) é a principal forma de doença periodontal destrutiva e resulta da interação entre bactérias e resposta inflamatória do hospedeiro, podendo ser afetada por fatores ambientais como o fumo. o objetivo do estudo foi avaliar a expressão de citocinas e quimiocinas, assim como a densidade de células dendríticas (CDs) imaturas e maduras, e densidade do infiltrado inflamatório no tecido gengival de indivíduos não-fumantes (NF) e fumantes (F), diagnosticados com periodontite crônica. O estudo foi aprovado pelo COEP-UFMG (423/11) e foram recrutados 24 indivíduos NF e 21 F...


Asunto(s)
Humanos , Masculino , Femenino , Citocinas/análisis , Fumar/efectos adversos , Periodontitis Crónica/complicaciones , Quimiocinas/análisis , Células Dendríticas/clasificación
20.
Braz Oral Res ; 27(6): 496-502, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24346047

RESUMEN

The aim of the present study was the morphometric evaluation of the epithelial lining and fibrous capsule in histological specimens of keratocystic odontogenic tumors (KOTs) before and after marsupialization. Histological sections from six KOTs that had undergone marsupialization followed by enucleation were photographed. The thickness and features of the capsule and of the epithelial lining of the tumor were evaluated upon marsupialization and upon subsequent enucleation using Axion Vision software. The histological specimens taken upon marsupialization presented an epithelial lining that is typical of KOTs. After marsupialization, the enucleated specimens had a modified epithelial lining and a fibrous capsule that both presented a greater median thickness (p = 0.0277 and p = 0.0212, respectively), morphological changes, and significant enlargement. These modifications can facilitate full surgical treatment and may well be related to a low KOT recurrence rate.


Asunto(s)
Enfermedades Maxilomandibulares/patología , Quistes Odontogénicos/patología , Adolescente , Adulto , Biopsia , Niño , Epitelio/patología , Femenino , Humanos , Enfermedades Maxilomandibulares/cirugía , Masculino , Quistes Odontogénicos/cirugía , Recurrencia , Estadísticas no Paramétricas , Factores de Tiempo , Resultado del Tratamiento , Adulto Joven
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