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1.
J Mater Sci Mater Med ; 32(1): 4, 2021 Jan 20.
Artículo en Inglés | MEDLINE | ID: mdl-33471194

RESUMEN

Adhesive resin-cements are increasingly used in modern dentistry. Nevertheless, released substances from resin materials have been shown to cause cellular toxic effects. Disc-shaped specimens from 12 different resin cements and one conventional zinc phosphate cement were prepared and used for direct stimulation of five different human cell lines via transwell cell culture system or in an indirect way using conditioned cell culture media. Cytotoxicity was determined using LDH and BCA assays. All tested cements led to a decrease of cell viability but to a distinct extent depending on cell type, luting material, and cytotoxicity assay. In general, cements exhibited a more pronounced cytotoxicity in direct stimulation experiments compared to stimulations using conditioned media. Interestingly, the conventional zinc phosphate cement showed the lowest impact on cell viability. On cellular level, highest cytotoxic effects were detected in osteoblastic cell lines. All resin cements reduced cell viability of human cells with significant differences depending on cell type and cement material. Especially, osteoblastic cells demonstrated a tremendous increase of cytotoxicity after cement exposure. Although the results of this in vitro study cannot be transferred directly to a clinical setting, it shows that eluted substances from resin cements may disturb osteoblastic homeostasis that in turn could lead to conditions favoring peri-implant bone destruction. Thus, the wide use of resin cements in every clinical situation should be scrutinized. A correct use with complete removal of all cement residues and a sufficient polymerization should be given the utmost attention in clinical usage.

2.
Clin Oral Investig ; 2021 Jan 28.
Artículo en Inglés | MEDLINE | ID: mdl-33506428

RESUMEN

OBJECTIVES: The aim of this study was, firstly, to analyse the long-time fatigue behaviour of crowns constructed from a novel polyetherketoneketone (PEKK) polymer, using artificial prepared teeth. Secondly, to determine the effect of the material's stiffness that used as an artificial prepared tooth on the fatigue life of the PEKK crowns in comparison to human prepared teeth. METHODS: Veneered crowns with a PEKK framework were constructed on three different prepared teeth: artificial polymethyl methacrylate (PMMA) teeth, artificial CoCr teeth and extracted human teeth. As far as applicable, the loading protocol was based on EN ISO 14801:2007 for fatigue testing of dental implants. After initial static fracture tests on three specimens from each group, the remaining crowns were loaded with different force levels until fracture or until 2 × 106 loading cycles were reached. The number of loading cycles until failure was recorded. Wöhler curves were created to display the fatigue limits. RESULTS: Static fracture limits as well as fatigue limits differed for all three core materials. The static fracture tests resulted in fracture limits of 1200 (± 293) N for the PMMA group, 1330 (± 219) N for the CoCr group and 899 (± 96) N for the human tooth group. Fatigue limits of 770 N, 840 N and 720 N were determined for the PMMA group, CoCr group and human tooth group, respectively. CONCLUSIONS: The determined fatigue limit of above 720 N (depending on the core material) is sufficiently high and a good performance of this crown material is expected in the clinical loading life. The results showed that using artificial teeth instead of natural teeth for fatigue testing of crowns might result in an overestimation of the fatigue limits of the crown material. CLINICAL RELEVANCE: PEKK-made crowns offer a stable and priceworthy treatment for patients, in particular those that suffer from metal allergy.

3.
Int J Comput Dent ; 23(3): 245-255, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32789312

RESUMEN

AIM: The aim of the present study was to evaluate the number, strength, and position of occlusal contacts shown using an intraoral scanner (IOS) and a digital occlusal analysis system (T-Scan) compared with the current gold standard using occlusal foil (OF). MATERIALS AND METHODS: Occlusal contacts were analyzed for 70 volunteers using OF in maximum intercuspation (MI). The contact points obtained using the IOS were evaluated using a screenshot from Zirkonzahn.Modellier CAD software. Finally, the volunteers were asked to bite on the sensor sheet of the T-Scan system. For the evaluation of these data, the contact points of the OF and the IOS were graded as light, medium, and strong. Furthermore, the positions of the contact points were analyzed for the anterior region (premolars and molars). Parametric statistical tests were applied to analyze the differences among the three methods. RESULTS: The mean number of all contact points was similar: 29 ± 8 with the OF, 30 ± 12 with the IOS, and 24 ± 10 with the T-Scan. However, results were different in terms of the grading of the strength of contact points: mean number of light contacts: 8 ± 4 OF vs 17 ± 8 IOS and 17 ± 6 T-Scan; medium contacts: 12 ± 5 OF vs 8 ± 4 IOS and 5 ± 4 T-Scan; and strong contacts: 9 ± 5 OF vs 6 ± 6 IOS and 4 ± 2 T-Scan. The positions of the occlusal contact points were also different. CONCLUSION: The data sets showed that there were differences in the distribution of occlusal contact points evaluated using the OF, the IOS, and the T-Scan system. Although the number of detected occlusal contacts was similar, different occlusal contact protocols were determined by the three different methods.


Asunto(s)
Oclusión Dental , Diente Molar , Diente Premolar , Humanos , Programas Informáticos
4.
Clin Oral Investig ; 24(11): 3997-4003, 2020 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-32246279

RESUMEN

OBJECTIVE: This is the second part of a report on tooth loss in Germany 1997-2030. Here, we describe trends in the prevalence of edentulism in seniors 1997-2014, assess predictive factors for edentulism, and projected it into 2030. MATERIAL AND METHODS: We used data from three waves of the cross-sectional, multi-center, nationwide representative German Oral Health Studies. Overall, 3449 seniors (65-74 years) were included (1997: 1367; 2005: 1040; 2016: 1042). Age, sex, educational level, smoking status, and the cohort were entered into age-cohort binary-logistic regression models to assess the association of predictors with edentulism and to project edentulism in 2030 via Monte Carlo simulations. RESULTS: Between 1997 and 2014, the prevalence of edentulism decreased from 24.8 to 12.4%. With each year of age, the risk of being edentate increased (by 11%, p < 0.001); it was also significantly increased in female versus male (by 40%, p = 0.001), low versus medium and high educational level (up to 257%, p < 0.001), and in former and current smokers (up to 258%, p < 0.001). We predict the prevalence of edentulism to be reduced to 4.2% in 2030. The reduction will be higher in males, never and former smokers, and those with low socio-educational level. On an absolute level and despite a growing elderly population (aged 60-80 years), the number of edentate individuals will have decreased by 3.6 million in 2030 compared with 1997. CONCLUSIONS: Edentulism in seniors has declined equitably in Germany. The decline is expected to continue until 2030. Further efforts are needed to tackle the underlying risk factors. CLINICAL RELEVANCE: This study presents trends of edentulism in Germany for a period of three decades. It provides clinically relevant data for health care planning by 2030.

5.
J Prosthodont Res ; 64(4): 498-505, 2020 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-32063531

RESUMEN

PURPOSE: This analysis focused on periodontal health in shortened dental arches (SDAs). METHODS: In a randomized controlled clinical trial, patients with missing molars in one jaw and at least one premolar and canine on both sides were eligible for participation. In the partial removable dental prosthesis (PRDP) group (n = 79), molars were replaced with a precision attachment retained PRDP. In the SDA group (n == 71), the SDA up to the second premolars was either left as is or restored with fixed dental prostheses. Outcome variables were vertical clinical attachment loss (CAL-V), pocket probing depth (PPD), bleeding on probing (BOP) and plaque index (PLI). For CAL-V and PPD, the changes at six measuring points per tooth were analyzed. For BOP and PLI, patient related rates were calculated for each point in time. Statistical methods included linear regression analyses. RESULTS: In the intention-to-treat (ITT) analysis for CAL-V in the study jaw, the 10 year patient related mean changes were 0.66 mm in the PRDP group and -0.13 mm in the SDA group. The resulting mean patient related group difference of 0.79 mm (95% CI: 0.20 mm-1.38 mm) was significant (p = 0.01). There were no significant differences in the ITT analyses for PPD. For BOP and PLI, significant group differences with more favorable results for the SDA group were found. CONCLUSIONS: In view of lacking substantial differences for CAL-V and PPD, the overall differences were considered of minor clinical relevance. The results add confirmatory evidence to the shortened dental arch concept and its clinical viability (controlled-trials.com ISRCTN97265367).


Asunto(s)
Dentadura Parcial Removible , Pérdida de Diente , Diente Premolar , Arco Dental , Humanos , Diente Molar
6.
Biomed Tech (Berl) ; 64(5): 555-562, 2019 Sep 25.
Artículo en Inglés | MEDLINE | ID: mdl-30875315

RESUMEN

The aim of this study was to investigate the retention forces of secondary telescopic crowns made of polyetherketoneketone (PEKK) in combination with primary crowns made of four different dental alloys and to determine whether the retention forces change in the course of up to 5000 simulated wear cycles. A total of four groups of telescopic crowns were investigated: group 1: 10 primary crowns made of a gold alloy (NEOCAST®3), group 2: 10 primary crowns made of a non-precious metal alloy (Girobond NB), group 3: 10 primary crowns made of zirconium (Cercon®base) and group 4: 10 primary crowns made from PEKK (Pekkton®ivory). The corresponding secondary crowns were made from PEKK in all the four groups. Each pair was fixed axially in a wear simulator specifically designed for the study. Overall, 10,000 joining and separating cycles were performed for each group. To simulate intraoral conditions, a saliva substitute served as a lubricant. Force transducers were used to record the retention forces and after completing 10,000 cycles, the surface of each primary crown was examined using a scanning electron microscope (SEM). All groups showed an increase in the retention force for the first 2000 cycles which stayed constant for the remaining 8000 cycles. The Pekkton®ivory/Pekkton®ivory and NEOCAST®3/Pekkton®ivory combinations displayed mean retention force values of 16 N after a slight increase in the retention force. The Cercon®base/Pekkton®ivory and Girobond NB/Pekkton®ivory combinations displayed an initially high increase in the retention force and then showed a mean retention force of up to 29 N. All primary crowns displayed surface wear. Zirconium primary crowns showed the least wear compared to PEKK and the gold and non-precious metal alloys.


Asunto(s)
Análisis del Estrés Dental/instrumentación , Aleaciones de Oro/química , Coronas , Aleaciones Dentales , Humanos , Circonio
7.
J Dent ; 80: 55-62, 2019 01.
Artículo en Inglés | MEDLINE | ID: mdl-30355509

RESUMEN

OBJECTIVES: To compare oral health-related quality of life (OHRQoL) in patients with either molar replacement by partial removable dental prostheses (PRDP) or with restored shortened dental arches (SDA) over a period of 10 years. METHODS: In this multi-center RCT, a consecutive sample of 215 patients with bilateral molar loss in at least one jaw was initially recruited in 14 prosthodontic departments. Of those patients, 150 could be randomly allocated to the treatment groups (SDA: n = 71; PRDP: n = 79), received the allocated treatment, and were available for follow-up assessments. OHRQoL was assessed using the 49-item version of the Oral Health Impact Profile (OHIP) before treatment (baseline) and at follow-ups after treatment (4-8 weeks and 6, 12, 24, 36, 48, 60, 96, and 120 months). To investigate the course of OHRQoL over time, we longitudinally modelled treatment and time effects using mixed-effects models. RESULTS: OHRQoL substantially improved from baseline to first follow-up in both groups indicated by a mean decrease in OHIP scores of 20.0 points (95%-CI: 12.5-27.5). When compared to the SDA group, OHRQoL in the PRDP group was not significantly different (-0.6 OHIP points; 95%-CI: -7.1 to 5.9) during the study period when assuming a constant time effect. OHRQoL remained stable over the 10 years with a statistically insignificant time effect (p = 0.848). CONCLUSIONS: For patients requesting prosthodontic treatment for their lost molars, treatments with SDA or PRDP improve clinically relevantly OHRQoL and maintain it over a period of 10 years with no option being superior to the other. CLINICAL SIGNIFICANCE: Since there was no significant difference between the two treatment options over the observation period of 10 years, and since results have stayed stable over time, patients can be informed that both treatment concepts are equivalent concerning OHRQoL.


Asunto(s)
Arco Dental , Dentadura Parcial Removible , Humanos , Salud Bucal , Calidad de Vida , Encuestas y Cuestionarios
8.
Cell Oncol (Dordr) ; 41(5): 485-494, 2018 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-29949049

RESUMEN

BACKGROUND: Targeting glucose metabolism is a promising way to interfere with tumor cell proliferation and survival. However, controversy exists about the specificity of some glucose metabolism targeting anticancer drugs. Especially the potency of STF-31 has been debated. Here, we aimed to assess the impact of the glucose transporter (GLUT) inhibitors fasentin and WZB117, and the nicotinamide phosphoribosyltransferase (NAMPT) inhibitors GMX1778 and STF-31 on tumor cell proliferation and survival, as well as on glucose uptake. METHODS: Tumor-derived A172 (glioblastoma), BHY (oral squamous cell carcinoma), HeLa (cervix adenocarcinoma), HN (head neck cancer), HT-29 (colon carcinoma) and MG-63 (osteosarcoma) cells were treated with fasentin, WZB117, GMX1778 and STF-31. Proliferation rates and cell viabilities were assessed using XTT, crystal violet and LDH assays. mRNA and protein expression of GLUT1 and NAPRT were assessed using qPCR and Western blotting, respectively. The effects of inhibiting compounds on glucose uptake were measured using [18F]-fluoro-deoxyglucose uptake experiments. RESULTS: Stimulation of tumor-derived cells with the different inhibitors tested revealed a complex pattern, whereby proliferation inhibiting and survival reducing concentrations varied in [18F]-fluoro-deoxyglucose uptake experiments more than one order of magnitude among the different cells tested. We found that the effects of GMX1778 and STF-31 could be partially abolished by (i) nicotinic acid (NA) only in nicotinic acid phosphoribosyltransferase (NAPRT) expressing cells and (ii) nicotinamide mononucleotide (NMN) in all cells tested, supporting the classification of these compounds as NAMPT inhibitors. In short-time [18F]-fluoro-deoxyglucose uptake experiments the application of WZB-117 was found to lead to an almost complete uptake inhibition in all cells tested, whereas the effect of fasentin was found to be cell type dependent with a maximum value of ~35% in A172, BHY, HeLa and HT-29 cells. We also found that STF-31 inhibited glucose uptake in all cells tested in a range of 25-50%. These data support the classification of STF-31 as a GLUT inhibitor. CONCLUSIONS: Our data reveal a dual mode of action of STF-31, serving either as a NAMPT or as a GLUT inhibitor, whereby the latter seems to be apparent only at higher STF-31 concentrations. The molecular basis of such a dual function and its appearance in compounds previously designated as NAMPT-specific inhibitors requires further investigation.


Asunto(s)
Anilidas/farmacología , Transporte Biológico/efectos de los fármacos , Proteínas Facilitadoras del Transporte de la Glucosa/antagonistas & inhibidores , Proteínas Facilitadoras del Transporte de la Glucosa/metabolismo , Glucosa/metabolismo , NAD/metabolismo , Piridinas/farmacología , Western Blotting , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Humanos , Hidroxibenzoatos/farmacología , Nicotinamida Fosforribosiltransferasa/metabolismo
9.
Int J Prosthodont ; 31(1): 77-84, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29316570

RESUMEN

PURPOSE: This study aimed to compare the long-term outcomes of two different nonimplant treatments in the bilateral shortened dental arch (SDA). MATERIALS AND METHODS: In a multicenter randomized controlled clinical trial, patients with complete molar loss in one arch were assigned to one of two different nonimplant treatments. In the partial removable dental prosthesis (PRDP) group, patients were provided with a distal-extension prosthesis retained with precision attachments. In the SDA group, patients were treated according to the SDA concept by preserving or restoring a premolar occlusion. RESULTS: Of the 152 treated patients, 82 reached the 10-year examination independent of their dental or prosthetic status. In the intention-to-treat analysis, the survival rates for tooth loss at 10 years were 0.44 (95% confidence interval [CI]: 0.30 to 0.56) in the PRDP group and 0.52 (95% CI: 0.37 to 0.65) in the SDA group. For tooth loss in the study arch, the survival rates were 0.67 (95% CI: 0.52 to 0.78) in the PRDP group and 0.60 (95% CI: 0.45 to 0.73) in the SDA group. The number of teeth lost was higher than expected. In a multivariate analysis using a multiple Cox regression model, the covariates age (unit: 1 year, Hazard Ratio [HR]: 1.033, P = .03) and DMFT value (unit: 1 tooth, HR: 1.121, P = .03) were significant for time to first tooth loss in the study arch. CONCLUSION: The results suggest an overestimation of the influence of the prosthetic management of the bilateral SDA. In treatment decisions, patient preferences should be considered with appropriate weight.


Asunto(s)
Arco Dental/patología , Dentadura Parcial Removible , Arcada Parcialmente Edéntula/rehabilitación , Pérdida de Diente , Coronas , Índice CPO , Pilares Dentales , Oclusión Dental , Ajuste de Precisión de Prótesis , Femenino , Humanos , Masculino , Diente Molar , Factores de Riesgo
12.
J Dent ; 65: 76-82, 2017 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-28711338

RESUMEN

OBJECTIVES: Dental resin-based materials are widely used in modern dentistry. Especially, resin cements enjoy great popularity and are utilized in many applications. Nevertheless, monomers could be released from the resinous matrix, thus interact with surrounding tissues, cause adverse biological reactions and may lead in cases of implant retained restorations to peri-implant bone destruction. Hence, we performed an in-vitro study to determine cytotoxicity of resin monomers on osteoblast-like cells. METHODS: Three permanent osteoblast-like cell lines from tumor origin (MG-63 and Saos-2) as well as immortalized human fetal osteoblasts (hFOB 1.19) were used and treated with different concentrations of the main monomers: BisGMA, UDMA, TEGDMA and HEMA. The impact on cell viability was monitored using three different cytotoxicity tests: alamarBlue, XTT, and LDH assay. Mean±SEM were calculated and statistical analysis was performed with GraphPad Prism software. RESULTS: All monomers tested caused concentration dependent cytotoxic effects on the three investigated osteoblast-like cell lines. Although all three cell viability assays showed comparable results in cytotoxic ranking of the monomers (BisGMA > UDMA > TEGDMA > HEMA), higher differences in the absolute values were detected by the various test methods In addition, also a cell line dependent influence on cell viability could be identified with higher impact on the immortalized hFOB 1.19 cells compared to both osteosarcoma cell lines (MG-63, Saos-2). CONCLUSIONS: Monomer concentrations detected in elution studies caused toxic effects in osteoblast-like cells. Although the results from in-vitro studies cannot be directly transferred to a clinical situation our results indicate that released monomers from composite resin cements may cause adverse biological effects and thereby possibly lead to conditions favoring peri-implantitis and bone destruction. CLINICAL SIGNIFICANCE: The wide use of composite resin cements especially in implant-prosthetic treatments should be scrutinized to avoid possible clinical implications between eluted resin monomers and bone cells leading to conditions favoring peri-implantitis and bone destruction.


Asunto(s)
Materiales Dentales/toxicidad , Ensayo de Materiales , Osteoblastos/efectos de los fármacos , Cementos de Resina/toxicidad , Resinas Sintéticas/toxicidad , Línea Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Relación Dosis-Respuesta a Droga , Humanos , Metacrilatos/toxicidad , Polietilenglicoles/toxicidad , Ácidos Polimetacrílicos/toxicidad , Poliuretanos/toxicidad , Factores de Tiempo
13.
Clin Oral Investig ; 21(6): 1945-1951, 2017 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-27785586

RESUMEN

OBJECTIVES: Being a secondary outcome in a multicenter randomized controlled trial, the present analysis focused on interdental spacing in the shortened dental arch (SDA). The aim was to evaluate changes in interdental spacing in dependence of two different treatments after an observation period of up to 5 years. MATERIAL AND METHODS: Patients were either treated with a partial removable dental prosthesis (PRDP) for molar replacement (PRDP group) or according to the SDA concept aiming at a premolar occlusion (SDA group) in a randomized manner. Interdental spacing in the anterior region was measured with gauges and categorized as "0" (<0.1 mm), "1" (<0.5 mm), "2" (0.5-1 mm), and "3" (>1 mm). The statistical analysis was performed with analysis of variance models followed by linear contrast. RESULTS: Ninety-one patients (SDA n = 41, PRDP n = 50) were included. Changes of interdental spacing were detected in 70.7 % of all cases. A significant difference between the mean score changes was found in the mandible comparing the PRDP group and the SDA group. The respective mean score changes from baseline to 5 years were 0.23 (SD 0.49) for the PRDP group and 0.02 (SD 0.30) for the SDA group (p = 0.023). CONCLUSIONS: Major interdental spacing could be observed in neither of the groups. The SDA concept resulted in a slightly better outcome. CLINICAL RELEVANCE: When deciding whether to replace missing molars, the present results give further support to the SDA concept.


Asunto(s)
Arco Dental/patología , Arcada Parcialmente Edéntula/rehabilitación , Oclusión Dental , Ajuste de Precisión de Prótesis , Dentadura Parcial Removible , Femenino , Humanos , Masculino , Diente Molar , Odontometría , Factores de Riesgo , Pérdida de Diente
14.
Arch Oral Biol ; 73: 142-150, 2017 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-27769028

RESUMEN

OBJECTIVE: The objective of this study was to investigate effects of insulin-like growth factor 1 (IGF1) on proliferation, wound healing and differentiation processes of human periodontal ligament (PDL) cells under inflammatory conditions and whether the protective, anabolic effects of IGF1 can attenuate unfavorable effects of interleukin-1ß (IL-1ß). DESIGN: Inflammation was mimicked through cell stimulation with IL-1ß. PDL cells were characterized in respect to the presence of components of the IGF system and the responsive potential on IL-1ß incubation. Gene expression levels were analyzed by quantitative real-time PCR. Cellular localization of target proteins was visualized using fluorescent-based immunohistochemistry. Effects on cell division were investigated by proliferation assays. Wound healing was analyzed using light microscopic techniques. Differentiation was quantified by measuring biomineralization and osteoblast-specific alkaline phosphatase enzyme activity. RESULTS: PDL cell proliferation and wound healing were positively affected by IGF1 and the combination of IGF1 with IL-1ß, while only IL-1ß showed negative effects. Biomineralization was enhanced by IGF1, IL-1ß, and the combination of both stimulants. Osteoblast differentiation was increased by IL-1ß and the combination of IL-1ß with IGF1, whereas only IGF1 negatively affected ALP activity. Phosphorylation of p38 was regulated by IL-1ß and IGF1. CONCLUSIONS: The data presented in this work showed a potential of IGF1 to improve wound healing and proliferation processes and to sustain cell differentiation under inflammatory stimuli in PDL cells.


Asunto(s)
Factor I del Crecimiento Similar a la Insulina/farmacología , Osteoblastos/efectos de los fármacos , Osteogénesis/efectos de los fármacos , Ligamento Periodontal/efectos de los fármacos , Cicatrización de Heridas/efectos de los fármacos , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismo , Fosfatasa Alcalina/metabolismo , Calcificación Fisiológica/fisiología , Diferenciación Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Activación Enzimática , Humanos , Factor I del Crecimiento Similar a la Insulina/metabolismo , Interleucina-1beta/antagonistas & inhibidores , Interleucina-1beta/metabolismo , Interleucina-1beta/farmacología , Osteoblastos/citología , Ligamento Periodontal/citología , Ligamento Periodontal/metabolismo , Transducción de Señal , Diente/citología , Diente/efectos de los fármacos , Tirfostinos/farmacología
15.
Biomed Tech (Berl) ; 62(1): 103-108, 2017 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-27071143

RESUMEN

Small diameter (mini) dental implants have become more popular in recent years as alternatives to classical implant treatment in clinical cases with critical bony situations. However, an in-depth scientific analysis of the mechanical and biomechanical effects of small diameter implants has not yet been published. The aim of the present study was to investigate experimentally different commercial mini implants by measuring their displacements under immediate loading. Twelve commercially available mini implants were measured. Implants were inserted into porcine mandibular segments and loaded by means of a predefined displacement of 0.5 mm of the loading system. The implants were loaded at an angle of 30° to the implant long axis using the self-developed biomechanical hexapod measurement system. Implant displacements were registered. The experimental results were compared to the numerical ones from a previous study. Measured implant displacements were within the range of 39-194 µm. A large variation in the displacements was obtained among the different implant systems due to the different designs and thread profiles. Comparing experimental and numerical results, the displacements that were obtained numerically were within the range of 79-347 µm. The different commercial mini implants showed acceptable primary stability and could be loaded immediately after their insertion.


Asunto(s)
Implantación Dental Endoósea/normas , Implantes Dentales , Diseño de Prótesis Dental/métodos , Análisis del Estrés Dental/métodos , Mandíbula/fisiología , Animales , Implantación Dental Endoósea/métodos , Implantes Dentales/normas , Diseño de Prótesis Dental/normas , Humanos , Mandíbula/química , Porcinos
16.
Int J Prosthodont ; 30(6): 595-597, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-27824981

RESUMEN

PURPOSE: To investigate the influence of framework materials with different elasticity moduli on the biomechanical performance of fixed partial dentures (FPDs). MATERIALS AND METHODS: A tooth-anchored three-unit FPD and surrounding tissues were modeled in two variants (veneered/full anatomical FPD) and loaded on the central unit. Three different framework materials (titanium, zirconia, high-performance polymer) were simulated. RESULTS: The polymer framework resulted in reduced framework stresses and increased veneering stresses. Varying the framework material had minimal influence on the loading of surrounding tissue. CONCLUSIONS: Using soft, novel materials as framework for dental FPDs does not negatively influence biomechanical loading of the involved biologic structures.


Asunto(s)
Coronas con Frente Estético , Diseño de Dentadura , Dentadura Parcial Fija , Pilares Dentales , Porcelana Dental , Análisis del Estrés Dental , Polímeros
17.
Dent Mater ; 32(8): 1052-64, 2016 08.
Artículo en Inglés | MEDLINE | ID: mdl-27323651

RESUMEN

OBJECTIVES: Resin infiltrants have been successfully used in dental medicine preventing the progression of tooth decay in an early phase of caries development. ICON(®) is an infiltrant of low-viscosity which penetrates via dentinal tubules into the lesion in dependence of the demineralization depth. Hence, we performed an in vitro study to determine the effect of ICON(®) on human dental pulp stem cells (hDPSCs). METHODS: Using explant technique, primary hDPSCs were collected from extracted teeth. Characterization and isolation were performed with typical mesenchymal stem cell markers (Stro-1, CD73, CD90, CD105) and hDPSCs differentiation was validated by immunofluorescence and flow cytometry. HDPSCs were stimulated with light-cured ICON(®) (lc) and non-light-cured ICON(®) (nc) conditioned media as well as different TEGDMA concentrations followed by the analysis of cytotoxicity, pro- and anti-inflammatory responses and differentiation using XTT assay, RT-PCR and ELISAs, respectively. RESULTS: Initial analysis demonstrated that hDPSCs express characteristic mesenchymal stem cell markers and differentiate into adipocytes, chondrocytes and osteoblasts. Notably, ICON(®) nc dramatically reduced cell viability (up to 98.9% after 48h), whereas ICON(®) lc showed only a modest cytotoxicity (10%). Data were in line with cytokine expression demonstrating increased levels of IL-6 and IL-8 as well as decreased IL-10 after ICON(®) nc exposure compared to ICON(®) lc. ICON(®) lc caused almost no alterations of DSPP, whereas ICON(®) nc markedly elevated DSPP mRNA levels (130.3-times). A concentration-dependent effect was observed in TEGDMA challenged hDPSCs. SIGNIFICANCE: ICON(®) is a successful minimal invasive technique. However, clinicians should strictly follow manufacturer's instructions to prevent adverse effects.


Asunto(s)
Pulpa Dental , Osteoblastos , Polietilenglicoles , Ácidos Polimetacrílicos , Diferenciación Celular , Humanos , Ensayo de Materiales , Células Madre Mesenquimatosas , Células Madre
18.
Eur J Oral Sci ; 124(3): 287-94, 2016 06.
Artículo en Inglés | MEDLINE | ID: mdl-27061513

RESUMEN

The purpose of this study was to investigate the corrosion behavior of 10 different high noble gold-based dental alloys, used for prosthodontic retention elements, according to ISO 10271. Samples of 10 high-noble and noble gold-based dental alloys were subjected to: (i) static immersion tests with subsequent analysis of ion release for eight different elements using mass spectrometry; (ii) electrochemical tests, including open-circuit potential and potentiodynamic scans; and (iii) scanning electron microscopy, followed by energy-dispersive X-ray microscopy. The results were analyzed using one-way ANOVA and Sidak multiple-comparisons post-hoc test at a level of significance of α = 0.05. Significant differences were found among the 10 alloys studied for all ions (P < 0.001). The potentiodynamic analysis showed values from -82.5 to 102.8 mV for the open-circuit potential and from 566.7 to 1367.5 mV for the breakdown potential. Both the open-circuit and the breakdown potential varied considerably among these alloys. Scanning electron microscopy analysis confirmed the existence of typically small-diameter corrosion defects, whilst the energy-dispersive X-ray analysis found no significant alteration in the elemental composition of the alloys. The results of this study reveal the variability in the corrosive resistance among the materials used for retention elements in prosthodontics.


Asunto(s)
Aleaciones Dentales , Prostodoncia , Corrosión , Oro , Aleaciones de Oro , Ensayo de Materiales , Propiedades de Superficie
19.
Cell Mol Life Sci ; 73(6): 1287-99, 2016 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-26407611

RESUMEN

In our study, ghrelin was investigated with respect to its capacity on proliferative effects and molecular correlations on oral tumor cells. The presence of all molecular components of the ghrelin system, i.e., ghrelin and its receptors, was analyzed and could be detected using real-time PCR and immunohistochemistry. To examine cellular effects caused by ghrelin and to clarify downstream-regulatory mechanisms, two different oral tumor cell lines (BHY and HN) were used in cell culture experiments. Stimulation of either cell line with ghrelin led to a significantly increased proliferation. Signal transduction occurred through phosphorylation of GSK-3ß and nuclear translocation of ß-catenin. This effect could be inhibited by blocking protein kinase A. Glucose transporter1 (GLUT1), as an important factor for delivering sufficient amounts of glucose to tumor cells having high requirements for this carbohydrate (Warburg effect) was up-regulated by exogenous and endogenous ghrelin. Silencing intracellular ghrelin concentrations using siRNA led to a significant decreased expression of GLUT1 and proliferation. In conclusion, our study describes the role for the appetite-stimulating peptide hormone ghrelin in oral cancer proliferation under the particular aspect of glucose uptake: (1) tumor cells are a source of ghrelin. (2) Ghrelin affects tumor cell proliferation through autocrine and/or paracrine activity. (3) Ghrelin modulates GLUT1 expression and thus indirectly enhances tumor cell proliferation. These findings are of major relevance, because glucose uptake is assumed to be a promising target for cancer treatment.


Asunto(s)
Ghrelina/metabolismo , Transportador de Glucosa de Tipo 1/metabolismo , Glucosa/metabolismo , Neoplasias de la Boca/metabolismo , Neoplasias de la Boca/patología , Boca/patología , Línea Celular , Línea Celular Tumoral , Proliferación Celular , Células Cultivadas , Regulación Neoplásica de la Expresión Génica , Ghrelina/análisis , Transportador de Glucosa de Tipo 1/análisis , Transportador de Glucosa de Tipo 1/genética , Glucógeno Sintasa Quinasa 3/metabolismo , Glucógeno Sintasa Quinasa 3 beta , Humanos , Boca/metabolismo , Neoplasias de la Boca/genética , ARN Mensajero/genética , Transducción de Señal , Células Tumorales Cultivadas , beta Catenina/metabolismo
20.
Lasers Med Sci ; 30(2): 475-81, 2015 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-23666547

RESUMEN

Heat generation during the removal of dental hard tissues may lead to a temperature increase and cause painful sensations or damage dental tissues. The aim of this study was to assess heat generation in dental hard tissues following laser ablation using an ultrashort pulse laser (USPL) system. A total of 85 specimens of dental hard tissues were used, comprising 45 specimens of human dentine evaluating a thickness of 1, 2, and 3 mm (15 samples each) and 40 specimens of human enamel with a thickness of 1 and 2 mm (20 samples each). Ablation was performed with an Nd:YVO4 laser at 1,064 nm, a pulse duration of 9 ps, and a repetition rate of 500 kHz with an average output power of 6 W. Specimens were irradiated for 0.8 s. Employing a scanner system, rectangular cavities of 1-mm edge length were generated. A temperature sensor was placed at the back of the specimens, recording the temperature during the ablation process. All measurements were made employing a heat-conductive paste without any additional cooling or spray. Heat generation during laser ablation depended on the dental hard tissue (enamel or dentine) and the thickness of the respective tissue (p < 0.05). Highest temperature increase could be observed in the 1-mm thickness group for enamel. Evaluating the 1-mm group for dentine, a significantly lower temperature increase could be measured (p < 0.05) with lowest values in the 3-mm group (p < 0.05). A time delay for temperature increase during the ablation process depending on the material thickness was observed for both hard tissues (p < 0.05). Employing the USPL system to remove dental hard tissues, heat generation has to be considered. Especially during laser ablation next to pulpal tissues, painful sensations and potential thermal injury of pulp tissue might occur.


Asunto(s)
Esmalte Dental , Pulpa Dental/efectos de la radiación , Dentina , Calor , Incisivo/cirugía , Terapia por Láser/métodos , Rayos Láser , Diente Molar/cirugía , Caries Dental/cirugía , Dureza , Humanos
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