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1.
J Infect Chemother ; 2021 Feb 12.
Artículo en Inglés | MEDLINE | ID: mdl-33589370

RESUMEN

We present the case of a patient with a voluminous cerebral abscess caused by Aggregatibacter aphrophilus and Actinomyces meyeri occurring a week post dental scaling. Both these bacteria are rarely involved in brain abscesses, and so far, cases of cerebral actinomyces have mostly been treated surgically and with intravenous (IV) antibiotics for 3-4 months, then put on oral antibiotic therapy with penicillin or amoxicillin for a further 3-12 months. Our patient underwent drainage through craniotomy and was subsequently put on intravenous ceftriaxone for 3 months accompanied by brain imaging control at the end of this period which showed complete regression of the abscess. Following parenteral treatment, no oral antibiotics were given since pharmacokinetic properties do not allow to attain high tissue concentration in the brain. This treatment gave excellent results.

2.
Genomics ; 113(1 Pt 1): 88-103, 2020 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-33271330

RESUMEN

Amaranth has been proposed as an exceptional alternative for food security and climate change mitigation. Information about the distribution, abundance, or specificity of miRNAs in amaranth species is scare. Here, small RNAs from seedlings under control, drought, heat, and cold stress conditions of the Amaranthus hypocondriacus variety "Gabriela" were sequenced and miRNA loci identified in the amaranth genome using the ShortStack software. Fifty-three genuine miRNA clustersthirty-nine belonging to conserved families, and fourteen novel, were identified. Identification of their target genes suggests that conserved amaranth miRNAs are involved in growth and developmental processes, as well as stress responses. MiR0005, an amaranth-specific miRNA, exhibited an unusual high level of expression, akin to that of conserved miRNAs. Overall, our results broaden our knowledge regarding the distribution, abundance and expression of miRNAs in amaranth, providing the basis for future research on miRNAs and their functions in this important species.

3.
J Glob Health ; 10(2): 020505, 2020 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-33110588

RESUMEN

Background: Epidemic outbreaks have significant impact on psychological well-being, increasing psychiatric morbidity among the population. We aimed to describe the early psychological impact of COVID-19 and its contributing factors in a large Spanish sample, globally and according to mental status (never mental disorder NMD, past mental disorder PMD, current mental disorder CMD). Methods: An online questionnaire was conducted between 19 and 26 March, five days after the official declaration of alarm and the lockdown order. Data included sociodemographic and clinical information and the DASS-21 and IES questionnaires. We analysed 21 207 responses using the appropriate descriptive and univariate tests as well as binary logistic regression to identify psychological risk and protective factors. Results: We found a statistically significant gradient in the psychological impact experienced in five domains according to mental status, with the NMD group being the least affected and the CMD group being the most affected. In the three groups, the depressive response was the most prevalent (NMD = 40.9%, PMD = 51.9%, CMD = 74.4%, F = 1011.459, P < 0.001). Risk factors were female sex and classification as a case in any psychological domain. Protective factors were younger age and ability to enjoy free time. Variables related to COVID-19 had almost no impact except for having COVID-19 symptoms, which was a risk factor for anxiety in all three groups. Conclusions: Our results can help develop coping strategies addressing modifiable risk and protective factors for each mental status for early implementation in future outbreaks.


Asunto(s)
Ansiedad/epidemiología , Infecciones por Coronavirus/psicología , Depresión/epidemiología , Trastornos Mentales/epidemiología , Neumonía Viral/psicología , Cuarentena/psicología , Adulto , Anciano , Anciano de 80 o más Años , Ansiedad/etiología , Betacoronavirus , Infecciones por Coronavirus/prevención & control , Estudios Transversales , Depresión/etiología , Femenino , Humanos , Modelos Logísticos , Masculino , Trastornos Mentales/etiología , Persona de Mediana Edad , Pandemias/prevención & control , Neumonía Viral/prevención & control , Prevalencia , España/epidemiología , Encuestas y Cuestionarios
4.
Molecules ; 25(18)2020 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-32942707

RESUMEN

Tomato (Solanum lycopersicum) is a widely consumed fruit all around the world. The industrial exploitation of tomato generates a lot of waste. Most of the utilization of tomato seeds waste is focused on animal feeding, as well as a food ingredient aimed to increase the protein content, and raw material for some organic bioactive component extraction. The aim of this work was to evaluate the techno-functional properties of tomato seed meal (TSM) and its nutraceutical properties after applying defatting processing (TSMD), and to evaluate the nutraceutical properties after a fermentation processing (TSMDF) by Lactobacillus sp. The results showed that, at alkaline conditions (pH 8-9), the techno-functional properties for TSM and TSMD improved. In comparison with TSM, TSMD showed higher water holding capacity (WHC ≈32%), higher oil holding capacity (OHC ≈13%), higher protein solubility (49-58%), more than 10 times foaming activity (FA), more than 50 times foam stability (Fst), as well as an improved emulsifying activity (EA) and emulsion stability (Est) wich were better at pH 9. Regarding the nutraceutical properties, after 48 h of fermentation (TSMDF), the antioxidant activity was doubled and a significant increase in the iron chelating activity was also observed. During the same fermentation time, the highest angiotensin-converting enzyme inhibition (ACEI) was achieved (IC50 73.6 µg/mL), more than 10 times higher than TSMD, which leads to suggest that this fermented medium may be a powerful antihypertensive. Therefore, the strategy proposed in this study could be an option for the exploitation of tomato wastes.

5.
Reumatol. clín. (Barc.) ; 16(4): 298-299, jul.-ago. 2020.
Artículo en Español | IBECS | ID: ibc-194958

RESUMEN

La vasculitis IgA es una vasculitis de pequeño vaso mediada por inmunocomplejos. Clínicamente se caracteriza por la púrpura palpable en miembros inferiores, la afectación articular en forma de artralgias o artritis y la afectación gastrointestinal y renal (esta última marcará el mal pronóstico en adultos). Es frecuente encontrar procesos infecciosos como desencadenantes, principalmente de vías respiratorias altas. Por otro lado, el VIH causa una disfunción inmunitaria que desencadena una hipergammaglobulinemia y puede desencadenar alteraciones autoinmunes. En ocasiones este efecto se realiza sobre el endotelio vascular dando lugar a cuadros vasculíticos, aunque como forma de inicio los casos descritos en la literatura son escasos


IgA vasculitis is a small-vessel vasculitis mediated by immune complexes. In clinical terms, it is characterized by palpable purpura in the lower limbs, joint involvement in the form of arthralgia or arthritis, and gastrointestinal and renal involvement (this will mark a poorer prognosis in adults). Infectious processes, mainly in the upper respiratory tract, are frequently found to be triggers. On the other hand, human immunodeficiency virus (HIV) causes immune dysfunction, which triggers hypergammaglobulinemia and can trigger autoimmune disorders. At times, this can affect the vascular endothelium, giving rise to vasculitic manifestations, although there are few reports in the literature of its role in the presentation of HIV


Asunto(s)
Humanos , Femenino , Adulto , Infecciones por VIH/complicaciones , Infecciones por VIH/diagnóstico , Vasculitis/etiología , Vasculitis/diagnóstico , Inmunoglobulina A/sangre
6.
Updates Surg ; 72(4): 1097-1103, 2020 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-32306274

RESUMEN

Severe acute pancreatitis complicated by infection is associated with high mortality. Invasive treatment is indicated in the presence of infected (suspected) pancreatic and/or peripancreatic necrosis (IPN) in the absence of response to intensive medical support. Step-up approach (SUA) has been demonstrated to lower complication rate compared to upfront open surgery. However, this approach has not been associated with lower mortality, and no factors have been studied that could help to identify the high risk patients. In this study, we aimed to analyse those factors associated with mortality following the invasive treatment of IPN, focusing on the role of surgical necrosectomy. A retrospective and observational study based on a multicentre prospective database was conducted. The database was coordinated by the Hospital General Universitario de Alicante, Spain and the Spanish Association of Pancreatology. Demographics, clinical data, and laboratory and imaging findings were collected. Atlanta 2012 criteria were considered to classify acute necrotizing pancreatitis and for the definition of IPN. Step-up approach was used in all centres with the intention of avoiding surgery whenever possible. Surgical necrosectomy was performed by open approach. From January 2013 to October 2014, a total of 1655 patients with the diagnosis of acute pancreatitis were included in our database. 1081 were recruited for the final analysis. Out of them, 205 (19%) were classified into acute necrotizing pancreatitis. 77 (8.3%) patients underwent invasive treatment of INP and were included in our study. Overall mortality was 29.9%. Upfront endoscopic or percutaneous drainage was performed in 60 (77.9%) patients and mortality was 26.6%. Out of 60, 22 (36.6%) patients subsequently received rescue surgery; mortality in rescue surgery group was 18.3%. Upfront surgery was carried out in 17 (22.1%) patients; mortality in this group was 41%. At univariate analysis, surgical necrosectomy, extrapancreatic infection, immunosuppression and de-novo haemodialysis were associated with mortality. At multivariate analysis, only surgical necrosectomy was significantly associated with mortality (p = 0.002 OR 3.89). Surgical approach for IPN is associated with high mortality rate. However, these data should be interpreted with caution, since we are not able to assess whether this occurs due to the need of surgery as the only resort when the other approaches are not feasible or fail.

7.
Front Med (Lausanne) ; 7: 126, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32328495

RESUMEN

Background/Objective: Evidence from basic and clinical studies suggests that unsaturated fatty acids (UFAs) might be relevant mediators of the development of complications in acute pancreatitis (AP). Objective: The aim of this study was to analyze outcomes in patients with AP from regions in Spain with different patterns of dietary fat intake. Materials and Methods: A retrospective analysis was performed with data from 1,655 patients with AP from a Spanish prospective cohort study and regional nutritional data from a Spanish cross-sectional study. Nutritional data considered in the study concern the total lipid consumption, detailing total saturated fatty acids, UFAs and monounsaturated fatty acids (MUFAs) consumption derived from regional data and not from the patient prospective cohort. Two multivariable analysis models were used: (1) a model with the Charlson comorbidity index, sex, alcoholic etiology, and recurrent AP; (2) a model that included these variables plus obesity. Results: In multivariable analysis, patients from regions with high UFA intake had a significantly increased frequency of local complications, persistent organ failure (POF), mortality, and moderate-to-severe disease in the model without obesity and a higher frequency of POF in the model with obesity. Patients from regions with high MUFA intake had significantly more local complications and moderate-to-severe disease; this significance remained for moderate-to-severe disease when obesity was added to the model. Conclusions: Differences in dietary fat patterns could be associated with different outcomes in AP, and dietary fat patterns may be a pre-morbid factor that determines the severity of AP. UFAs, and particulary MUFAs, may influence the pathogenesis of the severity of AP.

8.
Clin Exp Rheumatol ; 38(5): 925-932, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31969225

RESUMEN

OBJECTIVES: To compare the main characteristics of two inception cohorts (Italian [ITC] and Spanish [SPC]) cohorts of patients with systemic lupus erythematosus (SLE) at the time of diagnosis and at one year of follow-up. METHODS: Demographic, clinical and immunological characteristics, and treatments at SLE diagnosis and at 12 months of follow-up of ITC and SPC were compared. RESULTS: One hundred and sixty-four patients in the ITC and 231 patients in the SPC were compared. the patients from ITC were younger at SLE diagnosis (41.1±15.0 years vs. 46.4±15.6 years; p<0.001) and had a higher prevalence of arthritis (62.8% vs. 45.5%; p=0.001), serositis (25.6% vs. 16.0%; p=0.026), neurological involvement (7.9% vs. 1.7%; p=0.006), and immunological abnormalities (anti-dsDNA, anti-Sm, antiphospholipid antibodies) (93.9% vs. 77.8%; p<0.001). Conversely, photosensitivity (29.5% in ITC vs. 45.9% in SPC; p=0.001) and oral ulcers (12.4% vs. 30.3%; p<0.001) were more frequent at onset of SLE in the Spanish patients. At the first 12 months of follow-up, these differences were maintained. At SLE onset, more Italian patients received glucocorticoids (85.4% vs. 50.2%; p<0.001) and immunosuppressive agents. At 12 months of follow-up, more Spanish patients were treated with antimalarials (75.6% in ITC vs. 90.0% in SPC; p<0.001). Conversely, the use of glucocorticoids was lower in SPC (89.0% in ITC vs. 57.1% in SPC; p<0.001). CONCLUSIONS: These cohorts presented different profiles in terms of pattern of organ/system involvement and disease treatment, possibly as a consequence of patient selection or different disease management approaches between Italy and Spain.


Asunto(s)
Lupus Eritematoso Sistémico , Anticuerpos Antifosfolípidos , Humanos , Inmunosupresores/uso terapéutico , Italia/epidemiología , Lupus Eritematoso Sistémico/diagnóstico , Lupus Eritematoso Sistémico/tratamiento farmacológico , Lupus Eritematoso Sistémico/epidemiología , España/epidemiología
9.
Rev Argent Microbiol ; 52(1): 4-12, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31204058

RESUMEN

Currently, the valorization of agroindustrial waste is of great interest. Moringa oleifera is a multipurpose tree whose softwood residues could be used as raw material for low-cost cellulase production. The aim of this study was to isolate, identify, and characterize microorganisms with cellulolytic activity in different carbon sources. We isolated and purified 42 microorganisms from M. oleifera biomass. Fungi presenting the largest hydrolytic halos in carboxymethylcellulose as a substrate were molecularly identified as Penicillium funiculosum (FG1), Fusarium verticillioides (FG3) and Cladosporium cladosporioides (FC2). The ability of these fungal strains to break down cellulose was assessed in a submerged fermentation using either amorphous CMC or crystalline form (Avicel). P. funiculosum and C. cladosporioides displayed similar endoglucanase (606U/l) and exoglucanase (205U/l) activities in the Avicel-containing medium, whereas F. verticillioides showed the highest level of ß-glucosidase activity (664U/l) in the carboxymethylcellulose medium. In addition, the effect of three culture media (A, B, and C) on cellulase production was evaluated in P. funiculosum using moringa straw as a carbon source. The results showed a volumetric productivity improvement of cellulases that was 2.77-, 8.26-, and 2.30-fold higher for endoglucanase, exoglucanase and ß-glucosidase, respectively when medium C containing moringa straw was used as a carbon source. The enzymatic extracts produced by these fungi have biotechnological potential especially for second-generation bioethanol production (2G) from moringa straw. This is the first report on the use of M. oleifera biomass to induce the production of various cellulases in P. funiculosum.

10.
Reumatol Clin ; 16(4): 298-299, 2020.
Artículo en Inglés, Español | MEDLINE | ID: mdl-29776888

RESUMEN

IgA vasculitis is a small-vessel vasculitis mediated by immune complexes. In clinical terms, it is characterized by palpable purpura in the lower limbs, joint involvement in the form of arthralgia or arthritis, and gastrointestinal and renal involvement (this will mark a poorer prognosis in adults). Infectious processes, mainly in the upper respiratory tract, are frequently found to be triggers. On the other hand, human immunodeficiency virus (HIV) causes immune dysfunction, which triggers hypergammaglobulinemia and can trigger autoimmune disorders. At times, this can affect the vascular endothelium, giving rise to vasculitic manifestations, although there are few reports in the literature of its role in the presentation of HIV.

11.
Artículo en Inglés, Español | MEDLINE | ID: mdl-31864966

RESUMEN

BACKGROUND: There are few studies that relate eating and nutritional habits to the severity of the disease and demographic profile in patients with schizophrenia. OBJECTIVE: To describe eating and nutritional habits and their relationship with the severity of the disease in patients with schizophrenia. METHOD: Cross-sectional descriptive study. SAMPLE: 31 patients with schizophrenia (ICD-10) under outpatient treatment. INCLUSION CRITERIA: age 18-65 years, clinically stable and, written informed consent. ASSESSMENT: Demographic, clinical characteristics (CGI-SCH, length of illness, BMI, abdominal perimeter), ad hoc questionnaire (eating, nutritional, and physical activity). RESULTS: Mean age 43.13(SD=7.85) years, males 61.3%. Mean severity of illness was 3.94(SD=1.06), mean duration of the illness 18.42(SD=8.27) years. 74.2% used to eat weekly fat meat and 64.5% less than 3-4 servings of fish, 77.4% less than 3 servings of fruit per day, and 51.6% drink less than 1l of water. 83.9% used to drink coffee daily, 2.81(SD=2.02) cups per day. Patients showed lower levels of Vitamin A, D, E, K1, C, folic acid, and magnesium. 93.5% did not fulfill the WHO recommendations on physical activity. Only retinol (r=-0.602, p=0.039) and vitamin K1 (r=-0.693, p=0.012) in women were related to the severity of illness. CONCLUSIONS: Outpatients with schizophrenia do not follow WHO recommendations on healthy diets, neither physical activity. Both clinical severity of the illness and marital status and cohabitation were associated with poor eating habits and nutrients deficit. These data should be taken into account by the nursing staff when implementing specific care in routine clinical practice.

12.
PLoS One ; 14(7): e0219554, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31323038

RESUMEN

The potential of Pseudomonas putida KT2440 to act as a plant-growth promoter or as a bioremediator of toxic compounds can be affected by desiccation. In the present work, the bacterial survival ratio (BSR) in response to air desiccation was evaluated for P. putida KT2440 in the presence of different protectors. The BSR in the presence of nonreducing disaccharides, such as trehalose, was high after 15 days of desiccation stress (occurring at 30°C and 50% relative humidity), whereas in the absence of a protector the bacterial counts diminished to nondetectable numbers (ca 2.8 log CFU/mL). The LIVE/DEAD staining method showed that bacteria protected with trehalose maintained increased numbers of green cells after desiccation while cells without protection were all observed to be red. This indicated that nonprotected bacteria had compromised membrane integrity. However, when nonprotected bacteria subjected to 18 days of desiccation stress were rehydrated for a short time with maize root exudates or for 48 h with water (prolonged rehydration), the bacterial counts were as high as that observed for those not subjected to desiccation stress, suggesting that the cells entered the viable but nonculturable (VBNC) state under desiccation and that they returned to a culturable state after those means of rehydration. Interestingly an increase in the green color intensity of cells that returned to a culturable state was observed using LIVE/DEAD staining method, indicating an improvement in their membrane integrity. Cellular activity in the VBNC state was determined. A GFP-tagged P. putida strain expressing GFP constitutively was subjected to desiccation. After 12 days of desiccation, the GFP-tagged strain lost culturability, but it exhibited active GFP expression, which in turn made the cells green. Furthermore, the expression of 16S rRNA, rpoN (housekeeping), mutL, mutS (encoding proteins from the mismatch repair complex), and oprH (encoding an outer membrane protein) were examined by RT-PCR. All evaluated genes were expressed by both types of cells, culturable and nonculturable, indicating active molecular processes during the VBNC state.


Asunto(s)
Desecación , Pseudomonas putida/fisiología , Recuento de Colonia Microbiana , Proteínas Fluorescentes Verdes/metabolismo , Humedad , Microscopía Fluorescente , Oligonucleótidos , Raíces de Plantas/microbiología , ARN Ribosómico/metabolismo , ARN Ribosómico 16S/metabolismo , Rizosfera , Temperatura , Trehalosa , Zea mays/microbiología
13.
Appl Microbiol Biotechnol ; 103(6): 2493-2505, 2019 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-30693404

RESUMEN

Hypertension is a worldwide health problem. It is the main cardiovascular risk factor and affects about 31% of the world's adult population. The drugs used to control hypertension may cause side effects; for this reason, there are many investigations focused on searching for alternatives to control or prevent this disease through diet. For example, many peptides have demonstrated antihypertensive effects. The insertion of bioactive peptides is a biotechnological implement used to improve the nutraceutical properties of proteins. This work reviews the current data on the insertion of antihypertensive peptides (AHPs) into food proteins, the systems used to produce the AHPs, the advantages and disadvantages between them, the parameters to produce them at major scales, and their potential applications in pharmacy and functional foods.


Asunto(s)
Antihipertensivos/química , Suplementos Dietéticos/análisis , Alimentos Funcionales/análisis , Péptidos/química , Proteínas/química , Humanos , Hipertensión/tratamiento farmacológico , Hipertensión/prevención & control , Ingeniería de Proteínas
14.
Sci Total Environ ; 648: 66-79, 2019 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-30114590

RESUMEN

The monsoon season in South Korea has great influences on biogeochemical and hydrological processes in the entire country, but is specifically of concern in the Soyang lake watershed, the main drinking water reservoir for the 20-million-people metropolis Seoul. Therefore, water quality and nitrate concentration control in Lake Soyang is of high public priority. The Haean catchment is the most prominent agriculture-dominated sub-catchment of the Soyang lake watershed. It is a complex terrain influenced by extreme rain events and non-point nitrate sources. In this investigation we used input-output calculations and a stable isotope approach to quantify and determinate the origin of nitrate inputs into the rivers that later flow into the lake. During pre-monsoon and monsoon seasons in 2013 and 2014 we measured daily rainfall and river water discharge within the Haean catchment and collected rain, river water and groundwater samples in order to analyze nitrate concentrations and nitrate nitrogen and oxygen isotope abundances. Furthermore, we collected nitrogen fertilizers as applied in the catchment. Heavy monsoon events, as in 2013, were the most pronounced drivers of nitrate leaching being responsible for >80% of the nitrate output in the river runoff. On the other hand, an almost missing summer monsoon in 2014 drove the nitrate runoff in a different manner, being responsible for only 0.4% of the total nitrate nitrogen river discharge in the previous year. Results of nitrate nitrogen and oxygen isotope abundance analyses suggest soil microbial nitrification as the most important contributor to the nitrate in the river runoff. In addition, nitrate from groundwater partially affected by microbial denitrification contributed to the nitrate in the runoff due to river-aquifer exchange fluxes during the monsoon season. Direct leaching of nitrate from mineral fertilizers and atmospheric nitrate deposition were obviously only minor contributors to the nitrate in the runoff.

15.
Appl Microbiol Biotechnol ; 102(22): 9595-9606, 2018 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-30209550

RESUMEN

The insertion of peptides is a biotechnology tool widely used to improve the nutraceutical properties of proteins. Because the effect of these insertions in protein stability and function is difficult to predict, it should be determined experimentally. In this study, we created two variants of amarantin acidic subunit and analyzed them along with other four proteins reported previously. We measured their response against two destabilizing agents: temperature and urea. The six proteins presented the insertion of antihypertensive peptides (VYVYVYVY or RIPP) in the variable regions of the protein. We observed that their effect strongly depended on the site of the insertion. The insertion in the variable region I stabilized the protein both thermally and chemically, but it affected the inhibitory activity of the angiotensin-converting enzyme in vitro. In contrast, insertions in other three regions were severely destabilizing, producing molten globules. Our findings reveal that the insertion of bioactive peptides in variable regions of a protein can increase or decrease the protein's thermal and chemical stability and that these conformational changes may also alter its final activity.


Asunto(s)
Amaranthus/genética , Antihipertensivos/metabolismo , Péptidos/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Ingeniería de Proteínas/métodos , Estabilidad Proteica , Temperatura , Urea
16.
Arch. latinoam. nutr ; 68(2): 122-131, jun. 2018. tab
Artículo en Español | LILACS, LIVECS | ID: biblio-1016579

RESUMEN

Se han encontrado compuestos bioactivos en frutas y vegetales con efecto favorable en la prevención de enfermedades crónicas como cáncer y Alzheimer, que también están presentes en la quinua. Son necesarios realizar estudios para evaluar el potencial benéfico in vivo. El objetivo fue determinar la influencia del consumo de tres variedades de quinua (Chenopodium quinoa Willd.) sobre la acumulación de tejido adiposo y actividad antioxidante en tejidos de ratas obesas. 42 ratas obesas Holtzman fueron divididas en siete grupos. Con excepción del control, los otros grupos fueron alimentadas, durante 23 días, con dietas obesogénicas conteniendo 20% quinua variedad Altiplano, Pasankalla o Negra Collana, procesadas por cocción o tostado. Al finalizar el período de alimentación, la grasa visceral (GV) y abdominal (GA) fueron pesadas y se extrajo muestras de hígado e intestino delgado (ID) siendo almacenadas (­20°C) para posteriores análisis de actividad antioxidante. Se midieron capacidad antioxidante y contenido de polifenoles totales en quinuas procesadas. Se aplicó ANOVA utilizando el modelo lineal generalizado para diseño completamente randomizado con arreglo factorial 3x2+Control; y test de Fisher mediante el programa Minitabv.17.1.0. La GV, GA y peroxidación lipídica del ID no fueron afectadas significativamente (P>0.05) por las dietas experimentales; sin embargo, la peroxidación lipídica en el hígado de ratas que consumieron dietas con quinua fue significativamente menor (P<0.05) que el control. En conclusión, la alimentación de ratas obesas con dietas que contienen variedades de quinua procesada no afecta la acumulación de GV y GA; sin embargo, reduce la peroxidación lipídica en el hígado(AU)


Bioactive compounds with favorable effect in the prevention of chronic diseases such as cancer and Alzheimer are found in fruits and vegetables and are also present in quinoa. Studies are needed to evaluate the potential benefits in vivo. The objective was to determine the influence of the consumption of three varieties of quinoa (Chenopodium quinoa Willd.) on the accumulation of adipose tissue and antioxidant activity in tissues of obese rats. Forty two obese Holtzman rats were divided into seven groups. With exception of the control, the other groups were fed, during 23 days, with obesogenic diets containing 20% quinoa Altiplano, Pasankalla or Negra Collana, processed by cooking or roasting. At the end of the feeding period, visceral (VF) and abdominal fat (AF) were weighed and samples of liver and small intestine (SI) were extracted and stored (-20°C) for further analysis of antioxidant activity. Antioxidant capacity and total polyphenol content were measured in the processed quinoa. Applied ANOVA using the generalized linear model for a completely randomized design with a factorial arrangement 3x2+Control; and Fisher's test through the statistical program Minitab v.17.1.0. VF, AF and lipid peroxidation of SI were not significantly influenced (P>0.05) by the experimental diets; however, lipid peroxidation in the liver of rats consuming quinoa containing-diets was significantly lower (P <0.05) than control group. In conclusion, feeding obese rats with quinoa containing-diets do not affect the accumulation of VF and AF; however, it reduce lipid peroxidation in the liver(AU)


Asunto(s)
Ratas , Chenopodium quinoa/fisiología , Antioxidantes , Obesidad/fisiopatología , Tejido Adiposo , Dieta
17.
Rev. cuba. salud pública ; 43(3): 1-13, jul.-set. 2017. graf
Artículo en Español | LILACS, CUMED | ID: biblio-901534

RESUMEN

Introducción: En Cuba, la protección al adulto mayor es una prioridad, por cuanto constituye una política del estado la implementación de acciones multidisciplinarias e intersectoriales para el aumento de la calidad de vida, como reto a los desafíos del envejecimiento del siglo XXI. Objetivo: Valorar las acciones de promoción de salud de carácter gerontagógico para adultos mayores en la atención primaria de salud. Métodos: Se realizó un estudio mixto, transversal, en el período que abarca desde noviembre de 2013 a junio del 2016, en la Sede Universitaria de Ciencias Médicas de San José de las Lajas en la provincia Mayabeque. Se utilizaron métodos de análisis documental, descriptivos y triangulación. Se utilizó la información de investigaciones institucionales de intervención comunitaria con estudiantes de ciencias médicas, profesores de, la Universidad del Adulto Mayor y. factores comunitarios. Resultados: Se aplicaron acciones que favorecieron las relaciones comunitarias de apoyo social, la enseñanza con la práctica que mejoraron la calidad de vida de los adultos mayores, acorde con la voluntad política y acción intersectorial, como premisas clave de los resultados de salud que tiene el país. Conclusiones: Las acciones cumplen con los indicadores establecidos, contribuyen al desarrollo de capacidades, a un envejecimiento activo, satisfactorio, protagónico y al fortalecimiento de valores en recursos humanos para la vida social y laboral de forma útil(AU)


Introduction: The protection of the older adult in Cuba is a priority since the implementation of multidisciplinary and intersectoral actions to raise the quality of life and to prevent the consequences of aging in the 21 st century is a governmental policy. Objective: To assess the gerontagogic health promotion actions for older adults in the primary health care setting. Methods: A mixed cross-sectional study was conducted from November 2013 to June 2016 in the medical school of San Jose de las Lajas in Mayabeque province. Documentary and descriptive analyses as well as triangulation were the methods used in this study. The information collected from institutional community intervention research studies, which were conducted with medical students, professors, the University of the Older Adult, and community actors, were used. Results: The applied actions favored the community relationships of social support, the combination of teaching and practice improved the quality of life of the older adults in line with the political willingness and the intersectoral actions that represent a key premise for the health results achieved in the country. Conclusions: The gerontagogic actions meet the set indicators, contribute to the development of capabilities, to active and satisfactory aging and to the strengthening of values in human resources for useful social and working life(AU)


Asunto(s)
Atención Primaria de Salud , Salud del Anciano , Geriatría , Promoción de la Salud , Epidemiología Descriptiva , Estudios Transversales , Cuba
18.
Muscles Ligaments Tendons J ; 7(1): 192-199, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28717629

RESUMEN

BACKGROUND: Ultrasound imaging is often used by physiotherapists and other healthcare professionals but the reliability of image acquisition with different ultrasound machines is unknown. The objective was to compare the intra-rater, inter-rater and intermachine reliability of thickness measurements of the plantar fascia (PF), Achilles tendon (AT), patellar tendon (PT) and elbow common extensor tendon (ECET) with musculoskeletal ultrasound imaging (MSUS). METHODS: Tendon thickness was measured in four anatomical structures (14 participants, 28 images per tendon) by two sonographers and with two different ultrasound machines. Intraclass Correlation Coefficients (ICCs) and Bland-Altman plots were calculated. The standard error of measurement (SEM) and minimum detectable difference (MDD) were calculated. RESULTS: Inter-rater reliability was excellent for AT (ICC=0.98; 95% CI= 0.96-0.99) and very good for PT (ICC=0.85; 95% CI = 0.67-0.93) and ECET (ICC=0.81; 95% CI= 0.72-0.94). Reliability for PF was moderate, with an ICC of 0.63 (CI 95%= 0.20-0.83). Bland-Altman plot for inter-machine reliability showed a mean difference of 1 m for PF measurements and a mean difference of 4 m and 20 m for AT and PT. The relative SEMs were below 7% and the MDCs were below 0.7 mm. CONCLUSION: The MSUS reliability in measuring thickness of the four tendons is confirmed by the homogeneous readings intra sonographers, between operators and between different machines. Level of evidence: Tendon thickness can be measured reliably on different ultrasound devices, which is an important step forward in the use of this technique in daily clinical practice and research. LEVEL OF EVIDENCE: III.

19.
Rev. cuba. med. gen. integr ; 33(2)abr.-jun. 2017.
Artículo en Español | CUMED | ID: cum-74502

RESUMEN

Los indicadores demográficos se muestran en Cuba como aspecto relevante similar a los de países desarrollados. La reducción de la fecundidad, de la tasa anual de crecimiento de la población, de la mortalidad infantil y un incremento de la esperanza de vida al nacer, constituye avances en el desarrollo socioeconómico e influyen en los cambios poblacionales, determinados por variables demográficas de mortalidad, fecundidad y migraciones. Las personas de edad avanzada comparten cada vez más todos los escenarios, como parte de un proceso natural de la vida, pero es también indicador de aspiración humana legítima.1 Cuba es actualmente el país más envejecido de América Latina y también lo será en 2050 y en 2100, cuando habrá cuatro adultos mayores por cada niño, de acuerdo a las proyecciones de la Comisión Económica para América Latina y el Caribe (CEPAL).2 A la proporción de adultos mayores dentro de la población se añade la esperanza de vida de los cubanos, que es una de las más altas del mundo, con 78,45 años.3 Los datos demuestran que el envejecimiento de la población es un fenómeno palpable en la sociedad y que exhortan a mayores retos en su cuidado(AU)


Asunto(s)
Humanos , Anciano , Diabetes Mellitus Tipo 2 , Educación , Cuba
20.
Rev. cuba. med. gen. integr ; 33(2): 268-269, abr.-jun. 2017.
Artículo en Español | LILACS | ID: biblio-901173

RESUMEN

Los indicadores demográficos se muestran en Cuba como aspecto relevante similar a los de países desarrollados. La reducción de la fecundidad, de la tasa anual de crecimiento de la población, de la mortalidad infantil y un incremento de la esperanza de vida al nacer, constituye avances en el desarrollo socioeconómico e influyen en los cambios poblacionales, determinados por variables demográficas de mortalidad, fecundidad y migraciones. Las personas de edad avanzada comparten cada vez más todos los escenarios, como parte de un proceso natural de la vida, pero es también indicador de aspiración humana legítima.1 Cuba es actualmente el país más envejecido de América Latina y también lo será en 2050 y en 2100, cuando habrá cuatro adultos mayores por cada niño, de acuerdo a las proyecciones de la Comisión Económica para América Latina y el Caribe (CEPAL).2 A la proporción de adultos mayores dentro de la población se añade la esperanza de vida de los cubanos, que es una de las más altas del mundo, con 78,45 años.3 Los datos demuestran que el envejecimiento de la población es un fenómeno palpable en la sociedad y que exhortan a mayores retos en su cuidado(AU)


Asunto(s)
Humanos , Anciano , Diabetes Mellitus Tipo 2 , Educación
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