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J Orofac Pain ; 21(1): 7-18, 2007.
Artículo en Inglés | MEDLINE | ID: mdl-17312637


AIMS: To develop and validate a model in which to assess a loss of function associated with temporomandibular joint (TMJ) inflammation in awake, freely moving rats. METHODS: The dependent variable in the model was the time between food rewards (pellets), or interfeeding interval (IFI). IFI was quantified after rats were trained to "bar-press" for food. To validate use of the IFI as a surrogate for temporomandibular disorder (TMD) pain, we determined the impact of several manipulations, including changes in pellet size, the presence and severity of inflammation of the TMJ, masseter muscle, or skin (induced with complete Freund's adjuvant [CFA]), and the influence of preadministration of the non-steroidal anti-inflammatory drug indomethacin (4 mg/kg). Furthermore, in order to determine whether a change in IFI reflected an increase in the time rats spent eating, rats were videotaped, and the amount of time spent eating, grooming, and exploring was analyzed. RESULTS: Inflammation of the TMJ or masseter muscle resulted in significant dose- and pellet size-dependent increases in the IFI. Inflammation of the skin overlying the TMJ had no effect on IFI. Pre-administration of indomethacin reversed the inflammation-induced shift in the IFI. An inflammation-induced increase in IFI was associated with an increase in feeding time. CONCLUSIONS: Our model constitutes a relatively fast and sensitive method with which to assess changes in feeding behavior associated with TMJ inflammation. Only 2 days of training are required to obtain a stable baseline IFI. It is possible to detect changes in IFI as small as 40% with 12 rats per group.

Artritis Experimental/fisiopatología , Condicionamiento Operante/fisiología , Conducta Alimentaria/fisiología , Trastornos de la Articulación Temporomandibular/fisiopatología , Adyuvantes Inmunológicos/administración & dosificación , Animales , Antiinflamatorios no Esteroideos/uso terapéutico , Artritis Experimental/patología , Conducta Animal/fisiología , Colorantes , Dermatitis/patología , Dermatitis/fisiopatología , Modelos Animales de Enfermedad , Azul de Evans , Conducta Exploratoria/fisiología , Adyuvante de Freund/administración & dosificación , Aseo Animal/fisiología , Indometacina/uso terapéutico , Masculino , Músculo Masetero/patología , Músculo Masetero/fisiopatología , Miositis/patología , Miositis/fisiopatología , Premedicación , Ratas , Trastornos de la Articulación Temporomandibular/patología , Factores de Tiempo
J Neurophysiol ; 90(1): 515-20, 2003 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-12634279


Recent electrophysiological studies of cultured dorsal root and trigeminal ganglion neurons have suggested that multiple ionic mechanisms underlie the peripheral detection of cold temperatures. Several candidate "cold receptors," all of them ion channel proteins, have been implicated in this process. One of the most promising candidates is TRPM8, a nonselective cationic channel expressed in a subpopulation of sensory neurons that is activated both by decreases in temperature and the cooling compound menthol. However, evidence for the expression of TRPM8 in functionally defined cold-sensitive neurons has been lacking. Here, we combine fluorometric calcium imaging of cultured rat trigeminal neurons with single-cell RT-PCR to demonstrate that there are distinct subpopulations of cold responsive neurons and that TRPM8 likely contributes to cold transduction in one of them. TRPM8 is preferentially expressed within a subset of rapidly responsive, low-threshold (approximately 30 degrees C), cold-sensitive neurons. A distinct class of slowly responsive cold-sensitive neurons that is activated at lower temperatures (approximately 20 degrees C) generally lacks detectable TRPM8 mRNA. Together with previous findings, our data support the notion that cold responsive neurons are functionally heterogeneous.

Frío , Canales Iónicos/análisis , Proteínas de Neoplasias/análisis , ARN Mensajero/análisis , Ganglio del Trigémino/química , Animales , Regulación de la Temperatura Corporal , Calcio/fisiología , Señalización del Calcio , Técnicas de Cultivo de Célula , Cartilla de ADN , Fluorometría , Canales Iónicos/genética , Canales Iónicos/fisiología , Masculino , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/fisiología , Ratas , Ratas Sprague-Dawley , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Canales Catiónicos TRPM , Termorreceptores/fisiología , Ganglio del Trigémino/fisiología