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1.
Reprod Fertil Dev ; 32(8): 748-762, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-32362313

RESUMEN

Cattle undergo numerous environmental and management stressors that reduce fertility and affect ovulation. The extracellular matrix of the follicle wall can be altered by matrix metalloproteinases (MMPs), the activities of which are regulated by interleukins and tissue-specific inhibitors of metalloproteinases (TIMPs), especially during ovulation. The aims of the present study were to: (1) evaluate changes in the hormone milieu, the localisation and activity of MMP2 and MMP9 and the localisation of MMP14, TIMP1 and TIMP2 in response to adrenocorticotrophic hormone (ACTH) during the preovulatory period in cows; and (2) determine the direct effects of ACTH on the mRNA expression of MMP2 and MMP9 in the cultured follicle wall of bovine ovaries obtained from an abattoir. 100IU ACTH was administered during pro-oestrus every 12h until ovariectomy, which was performed before ovulation. Cortisol concentrations in the plasma and follicular fluid (FF) of preovulatory follicles were higher in ACTH-treated than control cows. Progesterone presented subluteal concentrations in plasma of ACTH-treated cows (P<0.05). MMP2 immunostaining and activity in ovaries were higher in ACTH-treated than control cows (P<0.05), whereas MMP9 immunostaining was similar between the two groups. However, unlike in control cows, MMP9 activity was absent in the FF of ACTH-treated cows. These results suggest that the administration of ACTH during the preovulatory period in cows could cause changes that culminate in modifications in the content and activation of MMPs and TIMPs in the ovary, which could interfere with the ovulation process.

2.
J Heart Lung Transplant ; 39(4S): S420-S421, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-32465689

RESUMEN

PURPOSE: Veno arterial extracorporeal membrane oxygenation (VA-ECMO) is an established rescue therapy for cardiogenic shock (CS). However, the increase in left ventricle (LV) afterload associated with inappropriate LV unloading remains an unsolved problem. Balloon atrial septostomy (BAS) is an inexpensive effective with low complication rates. Our purpose was to understand BAS effect in this setting. METHODS: Observational retrospective study, including patients submitted to BAS for LV unloading during VA-ECMO support due to CS between 2018 and 2019. Inotropic use was evaluated by the Wernovsky inotropic score. Statistical analysis was performed by median comparison with Wilcoxon test for related samples. RESULTS: Four patients with refractory CS despite VA-ECMO and intra aortic balloon pump (n=3) or Impella (n=1) support were included. The age of the patients was 55.5 years (IQR 46.8-64.3) and 75% were male. The basal median LV ejection fraction was 29.5%. All had severe functional mitral regurgitation and ischemic heart disease (n=3) or severe aortic stenosis (n=1). BAS was performed after a median time of 8 days (IQR 5.5-12.0) after ECMO with a final dimension of 5 mm. The reduction in left atrial pressure led to resolution of pulmonary edema in all patients. There was a hemodynamic improvement after the procedure, with lower inotropic use in 75% of patients (median difference -0,6; p= 1.00), and reduction in pulmonary pressures and lactate levels (Table 1). Additionally we documented an increase in LV outflow tract (LVOT) velocity time integral (VTI) and in the PaO2/FiO2 ratio. After a follow-up of 25.5 days (IQR 12.8-45.8) half of the patients were weaned from VA-ECMO or transplanted. One procedure was complicated by a femoral hematoma. CONCLUSION: Percutaneous BAS is a simple and effective procedure to treat refractory pulmonary edema during VA-ECMO support. The improvement in LV performance, documented by the increase in LVOT VTI, is likely due to improvement in intracavitary pressures and a better oxygenation.

3.
Artículo en Inglés | MEDLINE | ID: mdl-32394699

RESUMEN

We address here the role of oxidation impurities on the structure of graphene oxide films at the air-water interface by specular neutron reflectivity (SNR). We study films of purified graphene oxide (PGO) and nonpurified graphene oxide in the close-packed state. Nonpurified graphene oxide is constituted by graphene oxide (GO) layers with oxidation impurities adsorbed on the basal plane, while in PGO sheets, impurities are eliminated. SNR measurements show that GO films are formed by well-defined bilayers constituted by 2-3 layers of GO stacked in contact with air and a second layer of impurities submerged in the aqueous subphase. In contrast, PGO films are formed by a single layer in contact with air. We show for the first time that impurities constitute a layer submerged in the aqueous subphase, decrease the elasticity, and favor the collapse of graphene oxide films. Our results allow designing the surface properties of GO trapped at fluid interfaces.

5.
J Colloid Interface Sci ; 575: 119-129, 2020 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-32361044

RESUMEN

Upconversion nanoparticles (UCNP) are increasingly used due to their advantages over conventional fluorophores, and their use as resonance energy transfer (RET) donors has permitted their application as biosensors when they are combined with appropriate RET acceptors such as graphene oxide (GO). However, there is a lack of knowledge about the design and influence that GO composition produces over the quenching of these nanoparticles that in turn will define their performance as sensors. In this work, we have analysed the total quenching efficiency, as well as the actual values corresponding to the RET process between UCNPs and GO sheets with three different chemical compositions. Our findings indicate that excitation and emission absorption by GO sheets are the major contributor to the observed luminescence quenching in these systems. This challenges the general assumption that UCNPs luminescence deactivation by GO is caused by RET. Furthermore, RET efficiency has been theoretically calculated by means of a semiclassical model considering the different nonradiative energy transfer rates from each Er3+ ion to the GO thin film. These theoretical results highlight the relevance of the relative positions of the Er3+ ions inside the UCNP with respect to the GO sheet in order to explain the RET-induced efficiency measurements.

6.
J Healthc Qual Res ; 34(5): 233-241, 2019.
Artículo en Español | MEDLINE | ID: mdl-31713519

RESUMEN

OBJECTIVE: First to identify the areas of improvement in the surgical area before and during the performance of a surgical procedure in general surgery through the application of a Modal Analysis of Failures and Effects. Second to establish preventive measures to avoid adverse events in the surgical area. METHOD: A multidisciplinary working group was created in a university hospital for risk management in the General Surgery Operating Room Unit. The Modal Analysis of Faults and Effects was used. Potential risks for the patient in the ante-surgery and within the operating room were identified. The Risk Priority Index was calculated and preventive measures were established for all of them, with special interest when the Risk Priority Index was higher than 100. Preventive measures were developed based on the detected risks as well as those responsible for them. RESULTS: We identified a greater number of risks when the patient is in the operating room than in the ante-surgery room. Those with a higher risk priority index were: anticoagulated or antiaggregated patients, urinary tract infections, osteoarticular or neuropathic problems, patients not prepared for colon surgery, errors in laterality and leaving compresses in the operative field. CONCLUSIONS: A risk map has been developed in our organization, allowing the design of strategies to improve Patient Safety in the Surgical area. Training is a key aspect to improve Patient Safety.

7.
Theriogenology ; 138: 52-65, 2019 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-31301448

RESUMEN

Cystic ovaries (CO) characterize a disorder frequently found in dairy cattle. However, despite the contributions by several researchers, the mechanism that leads to ovulatory failure has not yet been completely elucidated. Thus, the aim of this study was to examine the mRNA expression of bovine vascular endothelial growth factor (VEGFA)-164, VEGFA-164b and VEGF receptors (VEGFR1 and VEGFR2) by real-time PCR and protein expression by immunohistochemistry, immunofluorescence and Western blot in follicular fluid from dairy cows with spontaneous CO and in an experimental model of follicular persistence induced by prolonged treatment with progesterone. Results showed that both VEGFA isoforms and receptors were coexpressed in granulosa and theca interna cells and in follicular fluid of ovaries from all the groups evaluated. VEGFA-164, VEGFA-164b and VEGFR2 protein expression was higher in theca cells of persistent follicles from group P0 (expected time of ovulation) than in those from dominant follicles (as reference structure) from the control group (p < 0.05). Also, VEGFA-164 expression was higher in theca cells of cysts than in those of dominant follicles of the control group (p < 0.05). In follicular fluid, VEGFA-164 expression was higher in persistent follicles from group P5 (5 days of follicular persistence) than in the control, P0 and P15 groups, and higher in cysts than in dominant follicles from the control group (p < 0.05). This study provides evidence of an altered expression of VEGFA-164, VEGFA-164b and VEGFR2 during the formation of persistent follicles and cysts in cows. Together, these results evidence that early development of CO in cows is concurrent with an altered expression of these growth factors and that these alterations may contribute to the follicular persistence, angiogenic dysregulation and ovulatory failure found in cows with follicular cysts.


Asunto(s)
Enfermedades de los Bovinos/genética , Enfermedades de los Bovinos/fisiopatología , Quistes Ováricos/genética , Quistes Ováricos/fisiopatología , Folículo Ovárico/fisiología , Factor A de Crecimiento Endotelial Vascular/fisiología , Animales , Estudios de Casos y Controles , Bovinos/fisiología , Enfermedades de los Bovinos/metabolismo , Femenino , Quiste Folicular/genética , Quiste Folicular/metabolismo , Quiste Folicular/fisiopatología , Expresión Génica , Quistes Ováricos/metabolismo , Ovario/metabolismo , Ovario/patología , Ovulación/genética , Ovulación/metabolismo , Receptores de Factores de Crecimiento Endotelial Vascular/metabolismo , Receptores de Factores de Crecimiento Endotelial Vascular/fisiología , Factor A de Crecimiento Endotelial Vascular/metabolismo
8.
Anim Reprod Sci ; 206: 1-10, 2019 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-31133358

RESUMEN

Throughout the estrous cycle the mammalian endometrium undergoes morphological and functional changes that are essential for the establishment of pregnancy and proper ovarian and uterine functions. Among these changes, the most important are alterations in both inter- and intracellular signalling molecules, many of which modulate immune processes. In the endometrial tissue there are local innate (nonspecific) and adaptive (specific/acquired) response mechanisms which vary because of the endocrine status during the estrous cycle, pregnancy and postpartum period. Endometrial cells have responses that support the immune system by producing pro-inflammatory factors such as cytokines, sensors, effector molecules and chemokines. This response is important during gestation, pregnancy, and fetal growth, as well as in preventing infection, and immuno-rejection of the semi-allogeneic embryo. In dairy cows, both before and immediately after calving, there are marked changes in the values for hormonal and metabolic variables and the immune status is impaired. Thus, in several studies there has been assessment of the physiological and/or abnormal maternal immune changes and possible effects on dairy cow reproductive performance. The objective with this review is to summarize the novel information about the immune mechanisms involved during the postpartum period, subsequent peri-implantation period and pregnancy in dairy cows, and the possible effects on reproductive performance. This information provides for an enhanced understanding of the local and systemic immune responses associated with the metabolic and hormonal status of dairy cows, and alterations in the immune system of high producing cows and the possible effects on subsequent fertility.


Asunto(s)
Bovinos/inmunología , Implantación del Embrión , Ciclo Estral/inmunología , Fertilidad/inmunología , Periodo Posparto/inmunología , Animales , Bovinos/fisiología , Femenino , Embarazo
9.
Rev. med. vet. zoot ; 66(1): 35-42, ene.-abr. 2019. tab
Artículo en Español | LILACS-Express | ID: biblio-1014235

RESUMEN

RESUMEN El objetivo de este estudio fue comparar el tiempo de cicatrización entre N-butil cianocrilato (NBC) y ácido poliglicólico (AC) sobre el cierre tisular en caninos sometidos a operaciones de esterilización. Se realizó un estudio observacional, comparativo y descriptivo sobre una población de 80 perros (Canis familiaris) vivos. Los animales se dividieron en cuatro grupos de 20 perros cada uno según sexo y material de sutura, así: primero grupo, hembras que recibieron NBC; segundo grupo, machos y NBC; tercero grupo, hembras y AC como medio de sutura en piel y cuarto grupo, machos y AC. La técnica quirúrgica en hembras fue oforosalpingohisterectomia y en machos orquiectomia. El tiempo de cierre fue estadísticamente (p < 0,05) menor en los grupos de machos y hembras con el adhesivo NBC comparado con los dos grupos tratados con AC. Se concluye que el NBC es un material seguro y eficaz como medio de fijación tisular en ambas técnicas quirúrgicas que disminuye los días de cierre y recuperación.


ABSTRACT The aim of this study was to compare N-butyl cyanoacrylate (NBC) and polyglycolic acid (AC) in tissue closure in canines subjected to sterilization operations. An observational, comparative and descriptive study was conducted in a population of 80 living dogs (Canis familiaris). The animals were divided into 4 groups of 20 dogs each, according to gender and suture material: the first group consisted of females that received NBC; the second group consisted of males that received NBC; the third group consisted of females that received AC as skin suture; and the fourth group was formed by males that received AC. The surgical technique in females was salpingo-oophorectomy, and in males it was orchiectomy. The closure time was statistically (p < 0.05) lower in the male and female groups with the NBC adhesive in comparison to the two groups treated with AC. It was concluded that NBC is a safe and effective material for tissue fixation in both surgical techniques, decreasing times of closure and recovery.

10.
J Healthc Qual Res ; 34(1): 12-19, 2019.
Artículo en Español | MEDLINE | ID: mdl-30733117

RESUMEN

OBJECTIVE: Creation and validation of a new in-house synthetic scale to measure patient safety culture. MATERIAL AND METHOD: Cross-sectional and descriptive study in which the results of the assessment of the level of safety culture in health and non-health professionals of a university hospital are collected using a new in-house synthetic scale as a measurement tool. It is called 'Questionnaire on patient safety culture in a Spanish speaking environment'. The construction process was carried out in six phases: Bibliographic search; Validation of the structure and content of the questionnaire by a group of experts in patient safety using a nominal group technique; Assumptions verification and exploratory factor analysis; Pilot test to ensure its compression by a convenience sample of expert professionals; Modification of version 1.1 after the relevant analyses and analysis of the reliability of the questionnaire. RESULTS: The final version of the questionnaire had nine items, grouped into three factors a priori: Hospital Management support in patient safety, Perception of Safety, and Expectations and actions of the Managers/Supervisors that favour safety. The items were structured using a 5-point Likert scale. A general assessment item on patient safety at the Centre was also included, as well as five open questions to identify actions on patient safety undertaken by the Centre. Finally, the possibility of making observations in a section of free text was included. The comprehension analysis did not recommend, in any case, the revision of the wording or modification of the items. The Spearman and Pearson indices were similar, which allowed us to assume the linearity in the relationships proposed. The Kolmogorov-Smirnov test was satisfactory in all cases, which guaranteed the normal distribution of the variables. The sample was adequate to perform the factorial analysis. Both the Bartlett' sphericity test and the Kaiser-Meyer-Olkin (KMO) index showed sample quality to perform the analysis. The recommendation of the exploratory factor analysis that advised eliminating 1 item was followed. Specifically, item 9 was eliminated: 'It is only a matter of luck that in my Centre no more errors occur that affect patients'. The analysis of our scale has shown that all the factorial loads were greater than 0.5, which indicates good explanatory capacity of the item for the Dimension. In total, the scale manages to explain more than 60% of the perception by professionals in patient safety, considering an acceptable loss of information. CONCLUSIONS: A new and validated in-house scale has been created to measure patient safety culture in the Spanish speaking healthcare environment.


Asunto(s)
Seguridad del Paciente , Administración de la Seguridad , Encuestas y Cuestionarios , Actitud del Personal de Salud , Estudios Transversales , Hospitales Universitarios , Humanos , Personal de Hospital/psicología , Reproducibilidad de los Resultados , España
11.
Gac Sanit ; 2019 Jan 11.
Artículo en Español | MEDLINE | ID: mdl-30642698

RESUMEN

OBJECTIVE: 1) To determine the perception of safety of health professionals and non-health professionals in a university hospital; 2) describe the climate of safety with its strengths and weaknesses; 3) evaluate the negatively valued dimensions and establish areas of improvement. METHOD: A cross-sectional and descriptive study carried out at the San Juan University Hospital in Alicante, where the results of the assessment of the safety culture level are collected using Hospital Survey On Patient Safety survey of the Agency for Healthcare Research and Quality adapted to Spanish language. RESULTS: The response rate was 35.36%. The group with the greatest participation was the physician (32.3%) and the service most involved, urgencies (9%). 86.4% had contact with the patient. 50% of workers rated the safety climate between 6 and 8 points. 82.8% did not report any adverse events in the last year. The professionals with the greatest security culture were the pharmacists and with the worst culture, the guards. No strength was identified globally. There were two dimensions that behaved like a weakness: 9 (staffing) and 10 (management support for patient safety). CONCLUSIONS: The patient's perception of safety is good, although it can be improved. No strengths have been identified. The weaknesses identified are staffing, management support for patient safety, handoffs and transitions, and safety perception.

12.
Opt Express ; 27(1): 196-201, 2019 Jan 07.
Artículo en Inglés | MEDLINE | ID: mdl-30645367

RESUMEN

We report a hybrid process by combining both vapor-phase and solution-doping techniques of rare-earth doped preform fabrication in conjunction with the MCVD technique, in order to fabricate highly efficient Tm-doped laser fibers. The proposed fabrication route takes advantage of co-doping silica with high alumina content through the vapor-phase doping process, which is otherwise difficult to achieve using conventional solution doping technique. In addition, by employing the solution doping method, high-purity thulium halide precursors that have low vapor pressures up to several hundred degree Celsius. These high-purity thulium halide precursors can be used to dope the fiber core region with a high thulium concentration that is optimized for an efficient two-for-one cross-relaxation process for 79xnm diode pumped thulium-doped fiber laser. Fibers fabricated using the hybrid approach show more homogeneous and flat-top dopant profiles, compared with the conventional approach, where both aluminum and thulium are incorporated in the core through solution doping. This will ensure that more doped region will take part in the cross-relaxation process. Superior laser performance with a slope efficiency of >70% in the two-micron band has been demonstrated when diode pumped at ~790nm.

13.
Dermatol Online J ; 24(7)2018 Jul 15.
Artículo en Español | MEDLINE | ID: mdl-30261562

RESUMEN

La alopecia areata constituye un reto terapéutico, sobre todo en sus formas extensas. Antes de iniciar cualquier tratamiento es necesario tener en cuenta algunas consideraciones. Se trata de una enfermedad que no afecta de forma directa a la salud del paciente y que puede presentar resolución espontánea. Las formas extensas, las que se inician en la infancia y las de larga evolución son muy rebeldes a los tratamientos y asocian recaídas. Todos los tratamientos tienen efectos secundarios. Ningún tratamiento ha demostrado alterar el curso de la enfermedad, muy pocos han demostrado eficacia en ensayos clínicos aleatorizados y no existen guías terapéuticas salvo la publicada en 2003 y actualizada en 2012 en el British Journal of Dermatology. Por todo ello, es necesario elaborar un plan de tratamiento individualizado en cada paciente. Se debe comenzar con los fármacos más seguros e inocuos, y pasar al siguiente escalón terapéutico cuando el actual haya demostrado su ineficacia durante un periodo de 6 meses. Se revisan las principales propuestas farmacológicas para alopecia areata, aportando datos sobre su mecanismo de acción, efectos secundarios y posicionamiento terapéutico en función de los estudios disponibles. Finalmente, se propone un algoritmo terapéutico como guía en el manejo de esta patología.


Asunto(s)
Corticoesteroides/uso terapéutico , Alopecia Areata/tratamiento farmacológico , Productos Biológicos/uso terapéutico , Factores Inmunológicos/uso terapéutico , Corticoesteroides/administración & dosificación , Alopecia Areata/terapia , Humanos , Quinasas Janus/antagonistas & inhibidores , Terapia por Láser , Minoxidil/uso terapéutico , Fototerapia , Plasma Rico en Plaquetas , Prostaglandinas/uso terapéutico , Inhibidores de Proteínas Quinasas/uso terapéutico , Vasodilatadores/uso terapéutico
14.
Animal ; 12(s2): s363-s371, 2018 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-30139395

RESUMEN

Epidemiological studies in humans and animal models (including ruminants and horses) have highlighted the critical role of nutrition on developmental programming. Indeed, it has been demonstrated that the nutritional environment during the periconceptional period and foetal development can altered the postnatal performance of the resultant offspring. This nutritional programming can be exerted by maternal and paternal lineages and can affect offspring beyond the F1 generation. Alterations in epigenetic mechanisms have been proposed as the causative link behind the programming trajectories observed in the offspring. Although a clear cause-effect relationship between epigenetic modifications during early development and later offspring phenotype has not been demonstrated in livestock species, strong associations have been reported for some epigenetic marks (e.g. messenger RNA) that are worth exploring as possible predictors of future offspring phenotype. In this review, we shortly describe the main epigenetic mechanisms studied so far in mammals (i.e. mainly in the mouse) thought to be associated with developmental programming, and discuss the few studies available in mammalian herbivores (e.g. cattle) showing the effect of nutrition on epigenetic marks and the associated phenotype. Clearly, there is a need to develop research on nutritional strategies capable of modulating the epigenetic machinery with positive influence on the phenotype of livestock herbivores. This type of research is needed to alleviate the challenges currently faced by the livestock industry (e.g. impaired fertility of high-yielding dairy cows). This in turn will have a positive influence on animal welfare and productivity of livestock enterprises.


Asunto(s)
Epigénesis Genética , Mamíferos/genética , Estado Nutricional , Animales , Bovinos , Femenino , Desarrollo Fetal , Herbivoria , Ganado , Mamíferos/embriología , Mamíferos/fisiología , Fenómenos Fisiologicos Nutricionales Maternos , Fenotipo , Embarazo , Rumiantes
15.
Biomed Res Int ; 2018: 9451547, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29992168

RESUMEN

The cattle tick Rhipicephalus (Boophilus) microplus is one of the most harmful ectoparasites affecting bovines worldwide. It represents a major threat to livestock industry due to the economic losses caused and diseases associated with these ticks. The most important tick control strategy has been the use of ixodicides, resulting in chemically resistant tick populations. It is necessary to understand the mechanisms that result in resistance so as to create new strategies increasing the lifespan of ixodicides or finding alternative targets to produce new acaricides. In this paper, in order to obtain an insight into the mechanisms that govern ixodicides resistance, we will compare the hemolymph proteome of two tick R. microplus strains, one susceptible (MJ) and one resistant (SA) to ixodicides, using HPLC and 2D electrophoresis. Significant differences were found in protein content between strains using HPLC. 2D electrophoresis revealed that 68 hemolymph protein spots were common between strains; however, 26 spots were unique to the susceptible strain MJ and 5 to the resistant strain SA. The most distinctive protein spots on the preparative gels were selected for further analyses. Nine protein spots were identified by mass fingerprinting, revealing proteins that may have a role in the ixodicides resistance or susceptibility. In this paper, we present the tick hemolymph proteome revealing a set of proteins which suggest a possible role in tick detoxification.


Asunto(s)
Acaricidas/farmacología , Hemolinfa/metabolismo , Proteómica , Rhipicephalus/enzimología , Animales , Bovinos , Enfermedades de los Bovinos , Femenino , Proteoma , Rhipicephalus/efectos de los fármacos
16.
J Fish Biol ; 92(5): 1333-1341, 2018 May.
Artículo en Inglés | MEDLINE | ID: mdl-29528107

RESUMEN

The effect of feed cycling (consisting of periods of starvation followed by periods of refeeding to satiation) on compensatory growth was evaluated in growth hormone transgenic and non-transgenic wild-type coho salmon Oncorhynchus kisutch. The specific growth rate (GSR ) of feed-restricted non-transgenic O. kisutch was not significantly different from the GSR of fully-fed non-transgenic O. kisutch during two refeeding periods, whereas the GSR of feed-restricted transgenic O. kisutch was significantly higher in relation to the GSR of fully-fed transgenic O. kisutch during the second refeeding period, but not during the first, indicating that growth compensation mechanisms are different between non-transgenic and growth-hormone (GH)-transgenic O. kisutch and may depend on life history (i.e. previous starvation). Despite the non-significant growth rate compensation in non-transgenic O. kisutch, these fish showed a level of body mass catch-up growth not displayed by transgenic O. kisutch.


Asunto(s)
Animales Modificados Genéticamente/crecimiento & desarrollo , Hormona del Crecimiento/genética , Oncorhynchus kisutch/crecimiento & desarrollo , Animales , Acuicultura , Femenino , Alimentos , Privación de Alimentos , Masculino , Oncorhynchus kisutch/genética , Inanición
17.
Bull Entomol Res ; 108(6): 765-772, 2018 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-29415782

RESUMEN

The potential of the mirid predator Dicyphus hesperus Knight (Heteroptera: Miridae) as a biological control agent of the sweetpotato whitefly, Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) and the potato psyllid, Bactericera cockerelli Sulcer (Hemiptera: Psyllidae) in tomato was investigated in two experiments. The first experiment focused on the study of the life history traits of D. hesperus when fed on nymphs of the potato psyllid compared with the factitious prey Ephestia kuehniella Zeller (Lepidoptera: Pyrallidae) eggs. Although reproductive and development rates were higher on E. kuehniella eggs, the predator exhibited a good intrinsic rate of natural increase (rm) when feeding on B. cockerelli nymphs (rm: B. cockerelli 0.069 ± 0.0001; E. kuehniella 0.078 ± 0.0001), thus reflecting good potential as a biocontrol agent of this pest. The second experiment focused on the efficacy of D. hesperus as a biocontrol agent of the potato psyllid and the sweetpotato whitefly in a tomato greenhouse. Prey species were offered individually or together in a series of five treatments in greenhouse cages. Results showed that the predator was able to establish and suppress populations of both pests inhabiting tomato plants when pests occurred alone or together. Thus, D. hesperus was demonstrated to be a suitable biocontrol agent of these two important pests that could be used in tomato greenhouses.


Asunto(s)
Hemípteros/fisiología , Control de Insectos/métodos , Rasgos de la Historia de Vida , Control Biológico de Vectores/métodos , Animales , Dieta , Femenino , Cadena Alimentaria , Heterópteros/fisiología , Lycopersicon esculentum/crecimiento & desarrollo , Masculino
18.
Theriogenology ; 110: 61-73, 2018 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-29334661

RESUMEN

Cystic ovarian disease (COD) is an important cause of infertility in dairy cattle. The main signs of this infertility are ovulation failure and follicular persistence. The aim of this study was to examine the expression of the cytokines IL-1ß, IL-1RI, IL-1RII, IL-1RA and IL-4 in ovarian follicular structures at different times of persistence in a model of follicular persistence induced by prolonged administration of progesterone in dairy cows. Protein expression of IL-1ß, IL-1RI, IL-1RII, IL-1RA and IL-4 was evaluated by immunohistochemistry. Additionally, IL-1ß and IL-4 concentrations in follicular fluid and serum were determined by ELISA. In granulosa cells, IL1-RII and IL-4 expression was higher in follicles with different persistence times than in the control dominant follicles. IL-1RA expression was higher in persistent follicles of the P15 group (15 days of follicular persistence) than in those of the control group. In theca cells, IL-1RII expression was higher in persistent follicles of the P0 group (expected time of ovulation) than in dominant follicles from the control group (p < .05) and the other persistence groups, whereas IL-4 expression was higher in persistent follicles of groups P0 and P15 than in the dominant follicles of the control group (p < .05). Differences between serum and follicular fluid within each group were detected only in P0 for IL-1ß, and in the control, P10 and P15 groups for IL-4 (p < .05). These results complement previous results, evidencing that early development of COD in cows is concurrent with an altered expression of cytokines in different ovarian follicular structures and may contribute to the follicular persistence and ovulation failure found in cattle with follicular cysts.


Asunto(s)
Anovulación/metabolismo , Bovinos/fisiología , Proteína Antagonista del Receptor de Interleucina 1/metabolismo , Interleucina-1beta/metabolismo , Interleucina-4/metabolismo , Folículo Ovárico/fisiología , Receptores Tipo II de Interleucina-1/metabolismo , Receptores Tipo I de Interleucina-1/metabolismo , Animales , Anovulación/veterinaria , Enfermedades de los Bovinos/metabolismo , Enfermedades de los Bovinos/fisiopatología , Supervivencia Celular , Industria Lechera , Femenino
19.
Phys Rev Lett ; 121(24): 242002, 2018 Dec 14.
Artículo en Inglés | MEDLINE | ID: mdl-30608729

RESUMEN

We report the first observation of the parity-violating gamma-ray asymmetry A_{γ}^{np} in neutron-proton capture using polarized cold neutrons incident on a liquid parahydrogen target at the Spallation Neutron Source at Oak Ridge National Laboratory. A_{γ}^{np} isolates the ΔI=1, ^{3}S_{1}→^{3}P_{1} component of the weak nucleon-nucleon interaction, which is dominated by pion exchange and can be directly related to a single coupling constant in either the DDH meson exchange model or pionless effective field theory. We measured A_{γ}^{np}=[-3.0±1.4(stat)±0.2(syst)]×10^{-8}, which implies a DDH weak πNN coupling of h_{π}^{1}=[2.6±1.2(stat)±0.2(syst)]×10^{-7} and a pionless EFT constant of C^{^{3}S_{1}→^{3}P_{1}}/C_{0}=[-7.4±3.5(stat)±0.5(syst)]×10^{-11} MeV^{-1}. We describe the experiment, data analysis, systematic uncertainties, and implications of the result.

20.
Rev. esp. patol ; 50(4): 257-261, oct.-dic. 2017. ilus
Artículo en Español | IBECS | ID: ibc-166044

RESUMEN

La granulomatosis eosinofílica con poliangeítis (Churg-Strauss) es una vasculitis sistémica con inflamación granulomatosa necrosante, rica en eosinófilos, a menudo con afectación del tracto respiratorio y vasculitis necrosante que afecta vasos pequeños y medianos, asociado con asma y eosinofilia. Presentamos un paciente cuya manifestación inicial de la enfermedad fue una colecistitis aguda alitiásica por vasculitis. Aunque la colecistitis aguda alitiásica como síntoma inicial de la granulomatosis eosinofílica con poliangeítis es muy infrecuente, se debería tener en cuenta en el diagnóstico en pacientes con dolor abdominal de origen desconocido con eosinofilia, asma o rinitis alérgica (AU)


Eosinophilic granulomatosis with polyangiitis (Churg-Stauss) is a systemic vasculitis with eosinophil-rich and necrotizing granulomatous inflammation and necrotizing vasculitis predominantly affecting small to medium vessels. It often involves the respiratory tract and is associated with asthma and eosinophilia. We describe a case in which acute acalculous cholecystitis was the initial manifestation of the disease. Although acute acalculous cholecystitis rarely appears as an early manifestation in eosinophilic granulomatosis with polyangiitis, this entity should be taken into consideration in the differential diagnosis of patients with abdominal pain of unknown origin and a history of eosinophilia, asthma, or allergic rhinitis (AU)


Asunto(s)
Humanos , Masculino , Adulto , Colecistitis Aguda/patología , Poliangitis Microscópica/complicaciones , Poliangitis Microscópica/patología , Síndrome de Churg-Strauss/patología , Vasculitis/patología , Vesícula Biliar/patología , Patología/métodos , Glomerulonefritis/complicaciones
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