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Indian J Dent Res ; 31(4): 550-556, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33107455


Background: The complex structure and irregularities of root canal walls are liable for infection by several bacterial species. Thus, the use of irrigants and auxiliary chemical solutions associated with instrumentation is necessary for effective eradication of the biofilm as well as complete removal of the smear layer. Aim: To evaluate the effects of calcium hypochlorite and chitosan oligosaccharide (COS) in disinfecting Enterococcus faecalis root canal biofilm and smear layer removal with minimal erosion. Materials And Methods: A total of 70 mandibular premolars were decoronated at the cementoenamel junction. The samples were biomechanically prepared, sterilized in an autoclave, and incubated with E. faecalis (ATCC-29212) bacteria for 21 days. Cleaning and shaping were done till maximum apical file size of #45 K. Specimens were randomly divided into 4 groups: GROUP I: Control Group, GROUP II: 5% Sodium Hypochlorite (NaOCl) solution followed by 17% EDTA solution, GROUP III: 5% Calcium Hypochlorite [Ca(OCl)2] solution followed by 17% EDTA solution and GROUP IV: 5% Ca(OCl)2 solution followed by 1% COS. The samples were subjected to microbial count followed by smear layer removal under scanning electron microscope (SEM) at coronal, middle and apical third. Statistical Analysis Used: Kruskal-Wallis Test and post-hoc Scheffe's test. Results: It was observed that Group IV showed the lowest amount of CFU count/mL and the highest amount of smear layer removal with a statistically significant difference (P < 0.05) when compared with the other three Groups. Conclusion: 5% Ca(OCl)2 solution with 1% COS solution effectively removed the Enterococcus faecalis biofilm and smear layer from the root canals with minimal erosion.

Quitosano , Capa de Barro Dentinario , Biopelículas , Compuestos de Calcio , Quitosano/farmacología , Ácido Edético , Humanos , Microscopía Electrónica de Rastreo , Oligosacáridos , Irrigantes del Conducto Radicular/farmacología , Preparación del Conducto Radicular
Indian J Dent Res ; 31(3): 470-474, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32769285


Objectives: To evaluate the leachable components of resin cements in oral simulating fluids using high performance liquid chromatography and its resultant shear bond strength with ceramics. Methods: Forty extracted permanent human mandibular molar teeth were sectioned horizontally with a disc beneath the dentino-enamel junction to expose the coronal dentin surface and later finished with 600-grit silicon carbide paper to create a uniform flat surface. Forty Lithium Di-silicate Ceramic block cylinders were sliced in a saw cutting machine at 250 rpm under water-cooling to obtain the discs. The teeth and the ceramic discs were then randomly assigned to two groups on the basis of material used for luting the ceramic disk. Group I: Ceramic disc luted with tooth surface using Variolink II Dual cure Resin Cement, Group II: Ceramic disc luted with tooth surface using multilink speed self-adhesive self-curing resin cement. All ceramic discs were etched with 5% HF acid (IPS Ceramic Etching Gel, Ivoclar, Schaan, Liechtenstein) for 20 seconds, then rinsed thoroughly for 20 seconds and dried for 20 seconds. Ceramic specimens were luted on dentin surfaces with the application of 5 kg load. The samples were stored in 75% ethanol solution for 2 weeks at 37 degree Celsius for chemical aging. HPLC Analysis were performed to analyze the eluted monomer. After HPLC Analysis, Samples were then loaded using universal testing machine for the evaluation of shear bond strength between ceramic discs and the resin cements before and after the elution of monomers. Results: Under HPLC analysis, results showed that the peak release of monomer is Bis-GMA in both the groups. The shear force required to break the bond between the ceramic luted to tooth surface with the resin cements were more for Group I. Statistical results: The statistical analysis was done using ANOVA and paired t-test and it shows P value ≥0.05, hence the null hypothesis is rejected. Conclusion: The monomer eluded from all the groups are identified as Bis-GMA, The eluded monomer decreases the bond strength between the resin cement and the ceramic due to water sorption, The Variolink II (dual cure) resin cement shows improved bond strength than the Multilink (self-cure self-adhesive) resin cement.

Recubrimiento Dental Adhesivo , Cementos de Resina , Cerámica , Cementos Dentales , Porcelana Dental , Análisis del Estrés Dental , Humanos , Ensayo de Materiales , Resistencia al Corte , Propiedades de Superficie
J Conserv Dent ; 20(5): 337-340, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-29386782


Aim: This study aims to evaluate the color change in human enamel bleached with three different concentrations of hydrogen peroxide, containing pineapple extract as an additive in two different timings, using reflectance spectrophotometer. Background: The study aimed to investigate the bleaching efficacy on natural teeth using natural enzymes. Materials and Methods: Baseline color values of 10 randomly selected artificially stained incisors were obtained. The specimens were divided into three groups of 20 teeth each: Group 1 - 30% hydrogen peroxide, Group II - 20% hydrogen peroxide, and Group III - 10% hydrogen peroxide. One half of the tooth was bleached with hydrogen peroxide, and other was bleached with hydrogen peroxide and pineapple extract for 20 min (Subgroup A) and 10 min (Subgroup B). Statistical Analysis: The results were statistically analyzed using student's t-test. Results: The mean ΔE values of Group IA (31.62 ± 0.9), Group IIA (29.85 ± 1.2), and Group IIIA (28.65 ± 1.2) showed statistically significant higher values when compared to the mean Δ E values of Group 1A (25.02 ± 1.2), Group IIA (22.86 ± 1.1), and Group IIIA (16.56 ± 1.1). Identical results were obtained in Subgroup B. Conclusion: The addition of pineapple extract to hydrogen peroxide resulted in effective bleaching.