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2.
J Colloid Interface Sci ; 596: 376-383, 2021 Mar 29.
Artículo en Inglés | MEDLINE | ID: mdl-33848744

RESUMEN

Photocatalytic applications of halide perovskites have attracted increasing attention. However, lack of stability and lead toxicity of lead halide perovskites have hindered their applications. Metal halide double perovskite (DP) Cs2AgInCl6 is a stable, environment-friendly semiconductor with direct band gap, and then the best promising alternative to lead halide perovskites. Here, the applications of Cs2AgInCl6 DP to photocatalytic degradation of organic pollutants have been developed, in which the octahedral Cs2AgInCl6 DP particles (~3.33 eV) were prepared by precipitation from acid solutions. The as-prepared samples exhibit high photocatalytic activity, which can degrade about 98.5% of water-insoluble carcinogen Sudan Red III in only 16 min, and have a good stability for 5 cycle operations. Furthermore, the Cs2AgInCl6 DP also can degrade Rhodamine B, Methyl orange and Methyl red efficiently, demonstrating a highly-efficient and stable ethanol solvent-based photocatalytic system for organic pollutants degradation. The high photocatalytic activity could be attributed to direct band gap and long carrier lifetime of Cs2AgInCl6 DP. These unique features of Cs2AgInCl6 DP indicate that it could have a good application prospect for photocatalytic degradation of organic pollutants.

3.
J Food Sci ; 2021 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-33856047

RESUMEN

This study investigated the influence of sarcoplasmic proteins (SPs) treated by the oxidation system (0.1 mmol/L FeCl3 , 0.1 mmol/L ascorbic acid, and 0, 1, 5, 10 mmol/L H2 O2 ) on the properties of pork myofibrillar proteins (MPs) gel. After oxidation treatment, the SPs showed an increased in carbonyl content and a decreased in total sulfhydryl content, coupled with the cross-linking of protein components by disulfide bonds and covalent bonds. The MPs gel with SPs oxidized at 1 mmol/L H2 O2 exhibited the maximal strength while the minimal water holding capacity (WHC). The WHC of MPs gel was significantly decreased with increasing SPs oxidation, leading to the increase of free water and the decrease of immobilized water in the gel system. The microstructures of MPs gels with moderately (1 mmol/L H2 O2 ) oxidized SPs showed a more compact and smaller pore gel network than MPs alone, suggesting adding oxidized SPs can expel water trapped in the gel. Furthermore, the environmental polarity of aliphatic C-H groups increased with SPs oxidation.

4.
Neural Regen Res ; 16(11): 2316-2323, 2021 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-33818518

RESUMEN

Although the transcriptional alterations inside the facial nucleus after facial nerve injury have been well studied, the gene expression changes in the facial nerve trunk after injury are still unknown. In this study, we established an adult rat model of facial nerve crush injury by compressing the right lateral extracranial nerve trunk. Transcriptome sequencing, differential gene expression analysis, and cluster analysis of the injured facial nerve trunk were performed, and 39 intersecting genes with significant variance in expression were identified. Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway analyses of the 39 intersecting genes revealed that these genes are mostly involved in leukocyte cell-cell adhesion and phagocytosis and have essential roles in regulating nerve repair. Quantitative real-time polymerase chain reaction assays were used to validate the expression of pivotal genes. Finally, nine pivotal genes that contribute to facial nerve recovery were identified, including Arhgap30, Akr1b8, C5ar1, Csf2ra, Dock2, Hcls1, Inpp5d, Sla, and Spi1. Primary Schwann cells were isolated from the sciatic nerve of neonatal rats. After knocking down Akr1b8 in Schwann cells with an Akr1b8-specific small interfering RNA plasmid, expression levels of monocyte chemoattractant protein-1 and interleukin-6 were decreased, while cell proliferation and migration were not obviously altered. These findings suggest that Akr1b8 likely regulates the interaction between Schwann cells and macrophages through regulation of cytokine expression to promote facial nerve regeneration. This study is the first to reveal a transcriptome change in the facial nerve trunk after facial nerve injury, thereby revealing the potential mechanism underlying repair of facial nerve injury. This study was approved by the Animal Ethics Committee of Nantong University, China in 2018 (approval No. S20180923-007).

5.
Hum Mol Genet ; 2021 Apr 02.
Artículo en Inglés | MEDLINE | ID: mdl-33822053

RESUMEN

FOXO1, a transcription factor downstream of the insulin/insulin like growth factor axis has been linked to protein degradation. Elevated expression of FOXO orthologs can also prevent aggregation of CAG-repeat disease causing polyglutamine (polyQ) proteins but whether FOXO1 targets mutant proteins for degradation is unclear. Here we show that increased expression of FOXO1 prevents toxic polyQ aggregation in human cells while reducing FOXO1 levels has the opposite effect and accelerates it. Although FOXO1 indeed stimulates autophagy, its effect on polyQ aggregation is independent of autophagy, UPS mediated protein degradation and is not due to a change in mutant polyQ protein turnover. Instead FOXO1 specifically downregulates protein synthesis rates from expanded pathogenic CAG repeat transcripts. FOXO1 orchestrates a change in the composition of proteins that occupy mutant expanded CAG transcripts, including the recruitment of IGF2BP3. This mRNA binding protein enables a FOXO1 driven decrease in pathogenic expanded CAG transcript- and protein levels, thereby reducing the initiation of amyloidogenesis. Our data thus demonstrate that FOXO1 not only preserves protein homeostasis at multiple levels, but also reduces accumulation of aberrant RNA species that may co-contribute to the toxicity in CAG-repeat diseases.

6.
Artículo en Inglés | MEDLINE | ID: mdl-33793194

RESUMEN

Organic redox-active molecules have been identified as promising cathodes for practical usage of potassium-ion batteries (PIBs) but still struggle with serious dissolution problems and sluggish kinetic properties. Herein, we propose a pseudocapacitance-dominated novel insoluble carbonyl-based cathode, [2,6-di[1-(perylene-3,4,9,10-tetracarboxydiimide)]anthraquinone, AQ-diPTCDI], which possesses high reversible capacities of 150 mAh g-1, excellent cycle stability with capacity retention of 88% over 2000 cycles, and fast kinetic properties. The strong intermolecular interactions of AQ-diPTCDI and in situ formed cathode electrolyte interphase films support it against the dissolution problem. The high capacitive-like contribution in capacities and fast potassium-ion diffusion enhance its reaction kinetics. Moreover, a symmetric organic potassium-ion battery (OPIB) based on AQ-diPTCDI electrodes also exhibits outstanding K-storage capability. These results suggest that AQ-diPTCDI is a promising organic cathode for OPIBs and provide a practicable route to realize high-performance K storage.

7.
Eur J Med Genet ; : 104212, 2021 Mar 29.
Artículo en Inglés | MEDLINE | ID: mdl-33794348

RESUMEN

BACKGROUD: Joubert syndrome is a rare neurodevelopmental disorder characterized by clinical and genetic heterogeneity. The characteristic molar tooth sign, resulted from cerebellar vermis hypoplasia and midbrain anomalies, is expected to be the key diagnostic feature for this disease, but it is difficult to make a definite diagnosis in prenatal only based on the imageology due to its clinical heterogeneity. CASE REPORT: We report on a fetus, who was detected cerebellum dysplasia and encephalocele by ultrasound at 19 and 23 weeks' gestation, confirmed by MRI examination. The pregnancy was terminated at 23 weeks' gestation. The induced fetus was identified postaxial polydactyly and deficient of occipital bone and skin. RESULTS: The whole exome sequencing identified a novel compound heterozygous variation in the Joubert syndrome related CPLANE1 gene including a 2-bp insertion, NM_023073.3:c.1383_1384dup;p.(Gly462Glufs*3) and a non-classic splicing variation, NC_000005.10(NM_023073.3):c.7691-5_7691-4del. The pathogenicity of the non-classic splicing variation was further confirmed by cDNA level sequencing, which showed a exon 39 skipping that would introduce a premature termination. The novel compound heterozygous variation caused a completed function loss of CPLANE1 gene. CONCLUSION: The cerebellum dysplasia fetus without obvious molar tooth sign was finally diagnosed as Joubert syndrome, combined with the results from genetic detecting and the postnatal clinical symptoms. We also highlight the clinical heterogeneity of encephalodysplasia in Joubert syndrome which increases the difficulty of clinical diagnosis especially for prenatal diagnosis. Our findings provides a new perspective for the prenatal diagnosis of Joubert syndrome with severe craniocerebral dysplasia and expanded the variation spectrum of CPLANE1 gene.

8.
J Am Dent Assoc ; 2021 Mar 29.
Artículo en Inglés | MEDLINE | ID: mdl-33795142

RESUMEN

BACKGROUND: The relationship of apical periodontitis (AP) and type 2 diabetes mellitus (T2DM) is poorly studied in large populations. The aims of this study were to determine if there is an independent association between AP and T2DM in a large hospital network after controlling for confounding variables, as well as to determine if glycated hemoglobin levels were independently associated with AP. METHODS: An initial search of the Carolina Data Warehouse for Health yielded 5,995,011 patients, of whom 7,749 were diagnosed with AP in 2015 through 2018. Patients' demographics, T2DM status, HbA1c, periodontal disease, oral cellulitis, hypertension, atherosclerosis, kidney disease, smoking, body mass index, the use of metformin or statins, and hospital inpatient status were collected from their most recent visit. A control group of 7,749 patients without AP were sampled and matched according to the age, race, and sex of each patient with AP. Multiple logistic regression was used to determine the association between T2DM and AP, as well as between HbA1c and AP after controlling for the effects of the aforementioned confounding variables, using a matched cohort design. RESULTS: T2DM was independently associated with significantly greater prevalence of AP (odds ratio [OR], 2.05; 95% confidence interval [CI], 1.73 to 2.43). The use of metformin (OR, 0.82; 95% CI, 0.69 to 0.98) or statins (OR, 0.70; 95% CI, 0.62 to 0.78) was independently associated with significantly lower prevalence of AP. HbA1c greater than 8.0 (OR, 2.46; 95% CI, 1.83 to 3.35) was significantly associated with greater prevalence of AP. CONCLUSIONS: T2DM and poorly controlled glycemia were significantly associated with AP. Metformin and statin use were associated with lower prevalence of AP. PRACTICAL IMPLICATIONS: This study provides evidence linking T2DM and the level of glycemia to the increased prevalence of AP. Statins and metformin use may be protective in this relationship.

9.
AAPS J ; 23(3): 50, 2021 03 31.
Artículo en Inglés | MEDLINE | ID: mdl-33791883

RESUMEN

Development of comprehensive and updated quantitative relationships between physiological parameters and age for pediatrics remains to be accomplished. Towards this goal, we have performed a thorough literature search and collected published data on organ weights and organ blood flow rates for 0-20-year-old male and female human subjects. The data were used to develop continuous relationships between physiological parameters and age, using a single form of mathematical equation. Four sets of equations (0-2 years male, 0-2 years female, 2-20 years male, 2-20 years female) for the body weight vs. age, height vs. age, and organ weight vs. age relationships and 2 sets of equations (0-20 years male, 0-20 years female) for organ flow rate vs. age relationship were developed. The variability of each physiological parameter was also estimated, and the equations allow simulation of a virtual population for a specific age, weight, and sex. We further compared the physiological parameters vs. age curves simulated using our equations to the existing databases (Simcyp Simulator and PK-Sim). The predicted physiological parameters were comparable between our study and the existing databases, validating our equation's utility. Additionally, we described body weight-normalized organ weights and organ blood flow rates as a function of age, to provide an insight into how the contribution of each organ towards total body weight and total blood flow changes throughout ontogenesis. The physiological parameter database and equations presented here can serve as an open source to facilitate the development of pediatric physiologically based pharmacokinetic models.

10.
Orphanet J Rare Dis ; 16(1): 159, 2021 Apr 07.
Artículo en Inglés | MEDLINE | ID: mdl-33827627

RESUMEN

BACKGROUND: Hypophosphatasia (HPP) is a rare inherited disorder, which is caused by loss-of-function mutations in the ALPL gene. HPP is a heterogeneous disease that has a wide spectrum of phenotypes. Few studies were carried out in the Chinese population with HPP, especially in children. METHODS: The clinical and genetic characteristics of 10 Chinese children with HPP who were referred to the Beijing Children's Hospital were described. Previously reported HPP cases of children in China were also reviewed. RESULTS: A total of 33 cases were identified, which included 2 perinatal lethal HPP, 10 infantile HPP, 10 childhood HPP, and 11 odonto HPP. The male-to-female ratio was 24:9. The average age at onset was 0.69 years (ranged from 2 h after birth to 14 years), while the average age at clinical diagnosis was 3.87 years (ranged from 2 h after birth to 19 years). Serum alkaline phosphatase (ALP) levels were significantly decreased in patients with perinatal lethal/infantile HPP when compared with those with the mild forms of HPP childhood/odonto HPP (P < 0.01). Although serum phosphate levels were not different (P > 0.05), serum calcium levels were elevated, and serum intact parathyroid hormone levels were decreased in patients with perinatal lethal/infantile HPP in comparison with those with the childhood/odonto HPP (P all < 0.01). Genetic analyses identified 40 mutations in 31 HPP cases, including 28 missense mutations, 9 frameshift mutations, 2 splice junction alterations, and 1 regulatory mutation. Of which, 5 novel mutations were identified in our present study: 2 frameshift mutations (p.Arg138GlyfsTer27, p.Leu511Profs*272); 2 missense mutations (p.Ala176Val, p.Phe268Leu), and 1 splice junction alteration (c.297+5G>A). Compound heterozygous mutations accounted for 80.6% of all variants. No mutational "hot-spot" was found. Most mutations of ALPL were located in exons 5, 7, 10, and 3. Notably, subjects that carrying single heterozygous mutations showed milder phenotypes of HPP, while subjects with nonsense mutations were associated with a severer phenotype. CONCLUSIONS: HPP is a rare disease with often delayed diagnosis, and the incidence of HPP in China may be seriously underestimated. The present study expands the phenotypic and genotypic spectrum and the understanding of HPP in Chinese children. These findings will be useful for clinical assessment and shorten the diagnosis time for pediatric HPP in China.

11.
Arthritis Res Ther ; 23(1): 102, 2021 04 07.
Artículo en Inglés | MEDLINE | ID: mdl-33827676

RESUMEN

BACKGROUND: Immunoglobulin G4-related disease (IgG4-RD) is a newly recognized systemic, immune-mediated, and fibro-inflammatory disease. Hypocomplementemia was found in part of IgG4-RD patients especially in the setting of active disease. OBJECTIVES: This study aimed to clarify the clinical features, treatment efficacy, and outcome in IgG4-RD patients with hypocomplementemia. METHODS: 312 IgG4-RD patients were recruited in our prospective cohort conducted in Peking Union Medical College Hospital. Patients were divided into hypocomplementemia group and normal complement group according to serum C3 and C4 levels measured at baseline before treatment. Low serum C3 levels (< 0.73 g/L) and/or C4 levels (< 0.10 g/L) were defined as hypocomplementemia. Demographic data, clinical characteristics, laboratory parameters, treatment, and outcome of two groups were analyzed and compared. RESULTS: Hypocomplementemia was identified in 65 (20.8%) cases of untreated IgG4-RD patients at baseline. The average age of hypocomplementemia group was 55.85 ± 10.89 years, with male predominance (72.3%). Compared with normal complement group, patients with hypocomplementemia were likely to have more involved organs, higher IgG4-RD responder index (IgG4-RD RI), and higher laboratory parameters such as counts of eosinophils, inflammatory markers, immunoglobulin G (IgG), IgG1, IgG3, IgG4, and IgE. In addition, lymph nodes, lacrimal gland, submandibular gland, parotid gland, paranasal sinus, bile ducts, and prostate gland were more commonly affected (p < 0.05). Serum C3 and C4 showed a significant positively correlation with each other. Both C3 and C4 were negatively correlated with the number of involved organs, IgG, IgG3, IgG4, and IgG4-RD RI, as well as positively correlated with IgA and hypersensitive C reactive protein (hsCRP). 64 (98.5%) patients responded quickly to initial therapy at a 3-month follow-up. Fifteen (23.1%) patients relapsed during follow-up with mean recurrence time of 14.2 ± 13.8 months. Compared with normal complement group, there was no significant difference of relapse rate in two groups (P = 0.401). CONCLUSIONS: Clinical characteristics of IgG4-related disease with hypocomplementemia differ from normal complement group. Serum C3 and C4 at baseline before treatment could be biological markers for disease activity. IgG4-RD with hypocomplementemia responded well to treatment and had no significant difference of relapse rate in IgG4-RD with normal complement.

12.
Bioresour Technol ; : 125101, 2021 Apr 06.
Artículo en Inglés | MEDLINE | ID: mdl-33858757

RESUMEN

Recently, anaerobic self-forming dynamic membrane bioreactors (AnSFDMBRs) have attracted increasing attention, and are considered as an alternative to conventional anaerobic membrane bioreactors (AnMBRs). The key advantages of AnSFDMBRs include high flux, low propensity towards fouling, and low capital and operational costs. Although there have been several reviews on AnMBRs, very few reviews on AnSFDMBR system. Previous AnSFDMBR studies have focused on lab-scale to investigate the long-term flux, methods to improve performance and the associated mechanisms. Microbial analysis showed that the phyla namely Proteobacteria, Bacteroidetes and Firmicutes are dominant in both bulk sludge and cake biofilm, but their abundance is low in biocake. This review critically examines the fundamentals of AnSFDMBRs, operational conditions, process optimization and applications. Moreover, the current knowledge gaps (e.g., dynamic membrane module optimization, membrane surface modification and functional microbes enrichment) that should be studied in future to design an efficient AnSFDMBR system for treatment of diverse wastewaters.

13.
Acta Odontol Scand ; : 1-9, 2021 Apr 10.
Artículo en Inglés | MEDLINE | ID: mdl-33843405

RESUMEN

OBJECTIVE: To establish and verify models predictive of thin periodontal phenotype and alveolar fenestration/dehiscence in the anterior teeth of patients with skeletal Class III malocclusion. MATERIAL AND METHODS: Retrospective data of 669 anterior teeth (305 in maxillae and 364 in mandibles) from 80 patients with skeletal Class III malocclusion before augmented corticotomy were collected. Distribution of thin periodontal phenotype and alveolar fenestration and dehiscence were evaluated and their associations with potential influencing factors were explored using univariate and multivariate analyses. The predictive models were visualized as nomograms, the accuracy of which was tested by receiver operating curve analyses. RESULTS: Thin phenotype was associated with Mazza bleeding index, sex, tooth type, probing depth and width of keratinized gingiva (WKG). Labial dehiscence was associated with age, jaw, labial bone thickness, mandibular plane angle, sagittal root position (SRP), sex, tooth type, and WKG. Labial fenestration was associated with sex, tooth type, SRP, and periodontal phenotype. The areas under the curves of nomogram prediction models for periodontal phenotype, alveolar dehiscence, and alveolar fenestration were 0.84, 0.81, and 0.73, respectively. CONCLUSIONS: Female sex, lateral incisor, and limited WKG may be risk factors for thin periodontal phenotype. Age, canine, male sex, mandible, thin labial bone thickness, and root positioned against the labial plate may be risk factors for labial dehiscence; and female sex, thick phenotype, root positioned against the labial plate, lateral incisor, and canine may be risk factors for labial fenestration. The predictive performance of the models was acceptable.

14.
Cancers (Basel) ; 13(5)2021 Mar 03.
Artículo en Inglés | MEDLINE | ID: mdl-33802279

RESUMEN

The brain is a common site of metastasis from advanced breast cancer but few effective treatments are available. We examined a therapeutic option with a conditioned medium (CM), focusing on the role of Lrp5 and ß-catenin in Wnt signaling, and IL1ra in osteocytes. Osteocytes presented the innate anti-tumor effect and the overexpression of the above genes strengthened their action. In a mouse model, the injection of their CM inhibited mammary tumors and tumor-driven osteolysis. Importantly, Lrp5- and/or IL1ra-overexpressing osteocytes or the local administration of ß-catenin-overexpressing CM markedly inhibited brain tumors. In the transport analysis, tumor-suppressing factors in CM were shown to diffuse through the skull. Mechanistically, the CM with overexpression of the above genes downregulated oncogenic genes such as MMP9, Runx2, TGFß, and Snail in breast cancer cells. Also, the CM with ß-catenin overexpression downregulated CXCL1 and CXCL5 and upregulated tumor suppressors such as LIMA1, DSP, p53, and TRAIL in breast cancer cells. Notably, whole-genome proteomics revealed that histone H4 was enriched in CM and acted as an atypical tumor suppressor. Lrp5-overexpressing MSCs were also shown to act as anti-tumor agents. Collectively, this study demonstrated the therapeutic role of engineered CM in brain tumors and the tumor-suppressing action of extracellular histone H4. The result sheds light on the potential CM-based therapy for breast cancer-associated brain metastases in a minimally invasive manner.

15.
Dev Comp Immunol ; 121: 104078, 2021 Mar 29.
Artículo en Inglés | MEDLINE | ID: mdl-33794278

RESUMEN

Host protective inflammatory caspase activity must be tightly regulated to prevent pathogens infection, however, the inflammatory caspase-engaged inflammasome activation in teleost fish remains largely unknown. In this study, we reveal a bifurcated evolutionary role of the inflammatory caspase in mediating both non-canonical and canonical inflammasome pathways in teleost fish. Through characterization of a unique inflammatory SmCaspase from the teleost Scophthalmus maximus (turbot), we found it can directly recognize cytosolic lipopolysaccharide (LPS) via its N-terminal CARD domain, resulting in caspase-5-like proteolytic enzyme activity-mediated pyroptosis in Turbot Muscle Fibroblasts. Interestingly, we also found that this inflammatory caspase can be recruited to SmNLRP3-SmASC to form the NLRP3 inflammasome complex, engaging the SmIL-1ß release in Head Kidney-derived Macrophages. Consequently, the SmCaspase activation can recognize and cleave the SmGSDMEb to release its N-terminal domain, mediating both pyroptosis and bactericidal activities. Furthermore, the SmCaspase-SmGSDMEb axis-gated pyroptosis governs the bacterial clearance and epithelial desquamation in fish gill filaments in vivo. To our knowledge, this study is the first to identify an inflammatory caspase acting as a central coordinator in NLRP3 inflammasome, as well as a cytosolic LPS receptor; thus uncovering a previously unrecognized function of inflammatory caspase in turbot innate immunity.

16.
Neuropsychologia ; 156: 107854, 2021 Apr 03.
Artículo en Inglés | MEDLINE | ID: mdl-33823163

RESUMEN

Transcranial direct current stimulation (tDCS) has great potential to modulate cortical excitability and further facilitate visual function or rehabilitation. However, tDCS modulation effects are largely variable, possibly because of the individual differences in initial performance. The present study investigated the influence of the initial performance on contrast sensitivity function (CSF) following tDCS. Fifty healthy participants were randomly assigned to three groups: anodal, cathodal and sham stimulation. The CSF was measured through a grating detection task before and immediately after tDCS. Active and reference electrodes were applied to the primary occipital cortex (Oz) and the middle of the head (Cz) for 20 min with an intensity of 1.5 mA, respectively. Compared with sham stimulation, anodal or cathodal stimulation had no effect on the area under the log CSF (AULCSF) or contrast sensitivity (CS) of various spatial frequencies at the group level. However, a negative relationship was found between initial performance and the AULCSF change (or CS change at a spatial of frequency 8 c/°) after the application of anodal tDCS, indicating that the degree of change was dependent on initial performance, with greater gains observed for those with poorer initial performance. Initial performance modulated the effect of anodal tDCS over the Oz on the CSF, indicating that the Oz plays a crucial role in visual function. These results contribute to a deep understanding of the mechanisms of tDCS and to the design of more precise and efficient personalized simulation approaches based on individual differences.

17.
Theranostics ; 11(11): 5143-5159, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33859739

RESUMEN

Background: Advanced breast cancer metastasizes to many organs including bone, but few effective treatments are available. Here we report that induced tumor-suppressing (iTS) MSCs protected bone from metastases while un-induced MSCs did not. Methods: iTS MSCs were generated by overexpressing Lrp5, ß-catenin, Snail, or Akt. Their tumor-suppressing capability was tested using a mouse model of mammary tumors and bone metastasis, human breast cancer tissues and cancer cell lines. Results: In a mouse model, the induced MSC-derived conditioned medium (MSC CM) reduced mammary tumors and suppressed tumor-induced osteolysis. Tumor-promoting genes such as CXCL2 and LIF, as well as PDL1, a blocker of T-cell-based immune responses were downregulated. Proteomics analysis revealed that heat shock protein 90 (Hsp90ab1), calreticulin (Calr) and peptidylprolyl isomerase B (Ppib), which are highly expressed intracellular proteins in many cancers, were enriched in MSC CM as atypical tumor suppressors. Thus, overexpressing selected genes that were otherwise tumorigenic rendered MSCs the tumor-suppressing capability through the atypical suppressors, as well as p53 and Trail. Notably, the inhibitory effect of Lrp5- and Akt-overexpressing MSC CMs, Hsp90ab1 and Calr presented selective inhibition to tumor cells than non-tumor cells. The development of bone-resorbing osteoclasts was also suppressed by MSC CMs. Conclusion: Collectively, the results showed an anti-tumor effect of iTS MSCs and suggested novel therapeutic approaches to suppress the progression of tumors into the bone.

18.
Eur Biophys J ; 2021 Apr 16.
Artículo en Inglés | MEDLINE | ID: mdl-33864101

RESUMEN

A small-scale ITC benchmarking study was performed involving 9 biophysics laboratories/facilities, to evaluate inter-laboratory and intra-laboratory basal levels of uncertainty. Our prime goal was to assess a number of important factors that can influence both the data gathered by this technique and the thermodynamic parameter values derived therefrom. In its first part, the study involved 5 laboratories and 13 different instruments, working with centrally prepared samples and the same experimental protocol. The second part involved 4 additional laboratories and 6 more instruments, where the users prepared their own samples according to provided instructions and did the experiments following the same protocol as in the first part. The study design comprised: (1) selecting a minimal set of laboratories; (2) providing very stable samples; (3) providing samples not requiring preparation or manipulation; and (4) providing a well-defined and detailed experimental protocol. Thus, we were able to assess: (i) the variability due to instrument and data analysis performed by each user on centrally prepared samples; (ii) the comparability of data retrieved when using 4 different software packages to analyze the same data, besides the data analysis carried out by the different users on their own experimental results; and (iii) the variability due to local sample preparation (second part of the study). Individual values, as well as averages and standard deviations for the binding parameters for EDTA-cation interaction, were used as metrics for comparing the equilibrium association constant (logK), enthalpy of interaction (ΔH), and the so-called "stoichiometry" (n), a concentration-correction factor.

19.
Eur Biophys J ; 2021 Mar 02.
Artículo en Inglés | MEDLINE | ID: mdl-33651123

RESUMEN

Mass photometry (MP) is a relatively new experimental technique with a quickly expanding list of applications. Using optical detection, MP measures the mass of individual molecules to obtain molecular mass distributions of proteins and other biomolecules in solution. The combination of speed, sensitivity, and very low sample consumption with label- and immobilization-free detection sets MP apart from other analytical methods. An increasing number of laboratories incorporates mass photometry as a routine sample analysis technique. However, MP measurements can sometimes be challenging, especially for users without previous experience with single-molecule techniques. Here, we present a protocol for the determination of protein molecular mass distributions by MP. It describes the sample and materials preparation as well as data collection and analysis. The advantages and limitations of this technique and the potential sources of artifacts are also given. This protocol can be used by new MP users and serve as a checklist for laboratories routinely performing MP experiments to guide consistent data collection and documentation.

20.
Exp Brain Res ; 2021 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-33646327

RESUMEN

The classification of inhibitory control and the relationship between the subcomponents of inhibitory control have been the focus of many studies. This study mainly explored the influence of response inhibition training on interference control through event-related potential data. Forty college students were randomly divided into a training group and a control group. Two response inhibition tasks were used as training tasks and the Stroop and go/no-go tasks were used with electroencephalogram monitoring to evaluate students' abilities in the two kinds of inhibitory control. The results showed that the conflict effect of the training group significantly improved after training compared with those of the control group. In the training group, the N2 effect was enhanced not only in the no-go stimulation in the training task but also in the incongruent stimulation in the untrained Stroop task and there was a correlation in the enhancement of the N2 effect between the two tasks. To some extent, this study provided neuroscientific evidence that response inhibition training can transfer to interference control.

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