Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 35
Filtrar
1.
Clin Oral Investig ; 24(9): 3017-3028, 2020 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-31853899

RESUMEN

OBJECTIVES: This study aimed to quantitatively compare the somatosensory function changes of inferior alveolar nerve (IAN) after mandibular third molar extraction with a surgery protocol of coronectomy, as opposed to the conventional method. MATERIALS AND METHODS: Patients with a lower third molar directly contacting IAN were recruited and assigned either to a test group (coronectomy group) or a control group (conventional extraction). A standardized quantitative sensory testing (QST) battery was performed for four times: one week before surgery and the second, seventh, and 28th days after surgery. Z-scores and the loss/gain coding system were applied for each participant. RESULTS: A total of 140 molars (test group: n = 91, control group: n = 49) were enrolled. The sensitivity of the mechanical detection threshold (MDT) and pressure pain threshold (PPT) significantly increased after surgery more than before surgery in both groups (P ≤ 0.001). After the surgery, the sensitivities of the cold detection threshold (CDT), cold pain threshold (CPT), and heat pain threshold (HPT) were significantly higher in the test group than in the control group (P ≤ 0.027). The risk of IANI was significantly larger (P = 0.041) in the test group than in the control group. CONCLUSIONS: QST was a sensitive way to detect somatosensory abnormalities even with no subjective complaint caused by surgery. Coronectomy had less influence on IAN function than conventional total extraction. CLINICAL RELEVANCE: The somatosensory function changes after mandibular third molar extraction were quantitatively studied, and coronectomy was proved a reliable alternation to reduce IAN injury rate.

2.
Chin J Dent Res ; 23(4): 265-271, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33491358

RESUMEN

Objective: To evaluate the accuracy of digital models obtained from intraoral scanning of edentulous maxilla and mandible models with and without resin markers. Methods: A pair of standard edentulous models were scanned using a laboratory scanner and saved as reference models. The edentulous models were fixed onto a phantom head and scanned with an intraoral scanner (IOS) five times each. Six resin markers were attached on the maxilla model and two on the mandible model, and another five intraoral scans were taken of each model. The scanning time and number of images were recorded. The digital models obtained using the IOS were superimposed on the reference models using image processing software. The trueness and precision of the models made using the IOS were evaluated, and the scanning time and number of images were also compared. Results: The average trueness and precision of the IOS in the maxilla model with resin markers were 135.50 ± 36.28 µm and 254.55 ± 40.62 µm, respectively, while those in the mandible were 161.40 ± 55.45 µm and 368.75 ± 91.03 µm, respectively. Placing resin markers on the edentulous maxilla and mandible did not improve the trueness of the IOS, but placing resin markers on the edentulous maxilla improved the precision and scanning efficiency. However, placing resin markers on the buccal shelf of the edentulous mandible decreased the precision and increased the scanning time. Conclusion: Resin markers placed on the hard palate of edentulous maxillae could improve the precision of the IOS and improve scanning efficiency. However, they did not affect the trueness of the IOS for edentulous maxillae or mandibles.


Asunto(s)
Técnica de Impresión Dental , Arcada Edéntula , Diseño Asistido por Computadora , Modelos Dentales , Humanos , Imagenología Tridimensional , Arcada Edéntula/diagnóstico por imagen
3.
J Oral Facial Pain Headache ; 33(3): 278­286, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30893407

RESUMEN

AIMS: To compare somatosensory function profiles and psychologic factors in patients with primary burning mouth syndrome (BMS) and healthy controls and to evaluate correlations of subjective pain ratings with somatosensory and psychologic parameters. METHODS: A quantitative sensory testing (QST) protocol-including cold detection threshold (CDT), warmth detection threshold (WDT), thermal sensory limen (TSL), paradoxical heat sensation (PHS), cold pain threshold (CPT), heat pain threshold (HPT), mechanical pain threshold (MPT), wind-up ratio (WUR), and pressure pain threshold (PPT)-was performed at the oral mucosa of the tongue, buccal, and palatal sites in 30 Chinese patients (25 women and 5 men, mean age 50.9 ± 9.2 years) with primary BMS and in 18 age- and gender-matched healthy controls (15 women and 3 men, mean age 53.2 ± 7.0 years). For each BMS patient, z scores and loss/gain scores were computed. Psychologic status was evaluated in both groups using the Self-Rating Anxiety Scale and Self-Rating Depression Scale. Correlations of BMS patients' subjective pain ratings with somatosensory and psychologic profiles were assessed with the use of Pearson or Spearman correlations and multiple linear regression. RESULTS: In BMS patients, 53.3% had somatosensory abnormalities according to z scores vs 22.2% of healthy controls (P = .033). The abnormalities in BMS patients were somatosensory loss to thermal nonnoxious stimuli (TSL = 20.0%, CDT = 13.3%, WDT = 13.3%), mechanical pressure stimuli (PPT = 16.7%), pinprick stimuli (MPT = 6.7%), and thermal pain stimuli (CPT = 3.3%), and somatosensory gain to repetitive pinprick stimuli (WUR = 6.7%), pressure stimuli (PPT = 6.7%), and thermal pain stimuli (HPT = 3.3%). The most frequent loss/gain score was 13.3% for loss of thermal somatosensory function with no somatosensory gain; 13.3% for loss of thermal and mechanical somatosensory function with no somatosensory gain; and 13.3% for gain of mechanical somatosensory function with no somatosensory loss. Mild elevations in anxiety scores were seen in 30% of the BMS patients, and 50% and 36.7% had mild and moderate elevations, respectively, in depression scores. No anxiety or depression was detected in the control group. QST results, but not psychologic scores, were significantly correlated with patients' subjective pain ratings (PHS, Spearman coefficient -0.384, P = .029; CPT, Pearson coefficient -0.370, P = .034; MPT, Pearson coefficient -0.376, P = .032; PPT, Pearson coefficient 0.363, P = .037). CONCLUSION: The present findings documented distinct differences in somatosensory function in patients with primary BMS compared to controls, indicating a complex pathophysiology and interaction between impairments in nociceptive processing and psychologic functioning.


Asunto(s)
Síndrome de Boca Ardiente , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Dolor , Dimensión del Dolor , Umbral del Dolor , Sensación Térmica
4.
Front Neurol ; 9: 649, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-30166977

RESUMEN

Temporomandibular disorders (TMDs) predominantly affect women of reproductive ages, with pain as the main symptom. The aim of the present study was to examine the effects of 17ß-estradiol (E2) on glutamate-evoked hypernociception of masseter muscle and to examine whether genistein could antagonize the effects of E2 in female rats. Injection of glutamate into the masseter muscle dose-dependently decreased head withdrawal thresholds, a parameter for mechanical hypernociception. Head withdrawal thresholds in ovariectomized rats also decreased with increasing doses of E2 replacement, and were further aggravated by injection of glutamate (1M, 40µL) into the masseters. Genistein at doses of 7.5 and 15 mg/kg antagonized E2-induced hypernociception of masseter muscle, and at doses of 7.5, 15, and 30 mg/kg also antagonized E2 potentiation of glutamate-evoked hypernociception of masseter muscle. Genistein produced optimal antagonistic effects of E2 on nociception behavior at a dose of 15 mg/kg. On the molecular level, tyrosine phosphorylation of the NR2B subunit of the N-methyl-D-aspartate receptor (pNR2B) and phosphorylated mitogen-activated protein kinase (pERK1/2) were significantly upregulated in the hippocampus following glutamate injection and were further potentiated by E2 replacement. Genistein at dose of 15 mg/kg partially reversed E2-potentiated glutamate-evoked upregulation of pNR2B and pERK1/2 expression in the hippocampus. These results indicated that moderate doses of genistein could antagonize E2 enhanced glutamate-evoked hypernociception of masseter muscle possibly via N-methyl-D-aspartate receptor and ERK1/2 signaling pathways in the hippocampus.

5.
J Headache Pain ; 17: 31, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-27071957

RESUMEN

BACKGROUND: The somatosensory phenotype of Chinese temporomandibular disorders (TMD) patients is not sufficiently studied with the use of contemporary techniques and guidelines. METHODS: A standardized quantitative sensory testing (QST) battery consisting of 13 parameters with a stringent statistical protocol developed by the German Research Network on Neuropathic Pain was performed over the most painful and corresponding contralateral sites as well as the right hand of 40 Chinese patients with TMD and pain classified according to the Diagnostic Criteria for TMD (DC/TMD). The same QST protocol was performed bilaterally over the infraorbital, mental, and hand regions of 70 age- and gender-stratified healthy Chinese controls. Z-scores and loss/gain scores were computed for each TMD patient. RESULTS: For patients, 82.5 % had somatosensory abnormalities in the painful facial region, while 60.0 % had abnormalities confined to the right hand. The most frequent abnormalities were somatosensory gain to pinprick (35.0 %) and pressure (35.0 %) stimuli, somatosensory loss to pinprick (25.0 %), cold (22.5 %), and heat (15.0 %) nociceptive stimuli. The most frequent loss/gain score was L0G2 (no somatosensory loss combined with a gain of mechanical somatosensory function) for both the facial (40.0 %) and hand (27.5 %) regions. Involving side-to-side differences in the evaluation increased the diagnostic sensitivity by 2.5-25.0 % across different parameters. CONCLUSIONS: Somatosensory abnormalities were commonly detected in Chinese TMD pain patients both within and outside the primary painful region, strongly indicating disturbances in the central processing of somatosensory stimuli. The individual variations in somatosensory abnormalities indicate a possible need for development of individualized TMD pain management.


Asunto(s)
Neuralgia/fisiopatología , Trastornos de la Sensación/fisiopatología , Trastornos de la Articulación Temporomandibular/fisiopatología , Adolescente , Adulto , Anciano , Femenino , Calor , Humanos , Masculino , Persona de Mediana Edad , Neuralgia/complicaciones , Dimensión del Dolor/métodos , Umbral del Dolor/fisiología , Estimulación Física , Trastornos de la Sensación/complicaciones , Trastornos de la Articulación Temporomandibular/complicaciones , Adulto Joven
6.
Beijing Da Xue Xue Bao Yi Xue Ban ; 48(1): 51-6, 2016 Feb 18.
Artículo en Chino | MEDLINE | ID: mdl-26885908

RESUMEN

OBJECTIVE: To investigate the relationship between the removal time of 0.2 mm occlusal interference and the recovery of masticatory muscle mechanical hyperalgesia in rats. METHODS: Forty male Sprague-Dawley rats (200-220 g) were randomly assigned to eight groups, with five rats in each group: (1) naive group: these rats were anesthetized and their mouths were forced open for about 5 min (the same duration as the other groups), but restorations were not applied; (2) sham-occlusal interference control group: bands were bonded to the right maxillary first molars which did not interfere with occlusion; (3)occlusal interference group: 0.2 mm thick crowns were bonded to the right maxillary first molars; (4) 2, 3, 4, 5, and 6 d removal of occlusal interference groups: 0.2 mm thick crowns were bonded to the right maxillary first molars and removed on days 2, 3, 4, 5, and 6. The naive group and sham-occlusal interference control group were control groups. The other groups were experimental groups. Bilateral masticatory muscle mechanical withdrawal thresholds were tested on pre-application days 1, 2, and 3, and on post-application days 1, 3, 5, 7, 10, 14, 21 and 28. The rats were weighed on pre-application day 1 and on post-application days 1, 2, 3, 4, 5, 6, and 7. RESULTS: Between the naive group and the sham-occlusal interference control group, there was no significant difference in the masticatory muscle mechanical withdrawal threshold of bilateral temporalis and masseters at each time point. No significant difference was detected between the contralateral side and ipsilateral side in experimental groups (P>0.05). In the 2, 3, 4, and 5 d removal of occlusal interference groups, the masticatory muscle mechanical withdrawal thresholds decreased after occlusal interference and increased after removal of the crowns and recovered to the baseline on days 7, 10, 14, and 14, respectively [the masticatory muscle mechanical withdrawal thresholds of right masseter muscle were (137.46 ± 2.08) g, (139.02 ± 2.11) g, (140.40 ± 0.98) g, (138.95 ± 0.98) g, respectively]. In the 6 d removal of occlusal interference group, the masticatory muscle mechanical withdrawal threshold increased after removal of the crowns and became stable since day 14. There was a significant difference between the 6 d removal of occlusal interference group and the sham-occlusal interference group on day 28 (P<0.05), the masticatory muscle mechanical withdrawal thresholds of right masseter muscle were (131.24 ± 0.76) g and (141.34 ± 1.43) g, respectively. CONCLUSION: After removal of the 0.2 mm thick crown within 5 days, the mechanical hyperalgesia of the rats could reverse completely. The mechanical hyperalgesia of the rats could only be relieved, but not reverse completely after removal of the 0.2 mm thick crown on day 6. As the time went on, even minor occlusal interference could cause irreversible mechanical hyperalgesia of masticatory muscles. This study suggested that occlusal interference caused by dental treatment should be eliminated as soon as possible, to avoid irreversible orofacial pain.


Asunto(s)
Oclusión Dental , Hiperalgesia/fisiopatología , Músculos Masticadores/fisiopatología , Animales , Coronas , Masculino , Músculo Masetero , Diente Molar , Dolor/prevención & control , Ratas , Ratas Sprague-Dawley , Factores de Tiempo
7.
Beijing Da Xue Xue Bao Yi Xue Ban ; 48(1): 94-100, 2016 Feb 18.
Artículo en Chino | MEDLINE | ID: mdl-26885916

RESUMEN

OBJECTIVE: To evaluate the effect of the occlusal surface height of a mandibular posterior implant-supported single crown on stress in bone tissues. METHODS: Three-dimensional finite element models of the implant-supported single crown replacing the missing right lower second premolar, mesial and distal natural teeth, periodontal membrane, alveolar bone, loaded rigid body and analog food of almond were established. Using the Federation Dentaire International (FDI) system, the first premolar, the second premolar and the first molar were represented with 44, 45, 46. Three occlusal surface heights of the crown were studied: (1) normal height; (2) 15 µm reduction in height; (3) 30 µm reduction in height. The models were loaded by independent loading with maximal occlusal force(44 by 280 N, 45 by 360 N, and 46 by 480 N) and average occlusal force(44 by 140 N, 45 by 180 N, and 46 by 240 N)on the single crown; combined loading (maximal occlusal force transformed into uniform load of 3.7 MPa on top of rigid body, in contact with points on the occlusal surface), and analog almond-like food loading (average occlusal force transformed into uniform load of 1.67 MPa in simulated food chewing, in contact with points on the occlusal surface). RESULTS: For maximal biting force under independent loading, Von Mises stress peak values in bone tissues around 44, 45, and 46 were 82.57 MPa, 45.26 MPa and 27.79 MPa; For average biting force, peak values were 41.28 MPa, 22.63 MPa and 13.89 MPa. Under combined loading, compared with the normal occlusal surface height group, Von Mises stress peak values decreased 4.6 MPa, by 0.84%; increased 7.52 MPa, by 20.04%, and decreased 1.8 MPa, by 5.84%, for 45, 46, and 44 in the 30 µm infra-occlusion group, respectively. Under food loading, Von Mises stress peak values decreased 0.34 MPa, by 1.62%; increased 1.11 MPa, by 2.66%; and increased 0.06 MPa, by 0.54%, and for 45, 46, and 44 in the 30 µm infra-occlusion group, respectively. CONCLUSION: Within the limitation of this study, within 30 µm reduction of the occlusal surface height of implant-supported single crown, no significant difference of the peak values was observed.


Asunto(s)
Coronas , Prótesis Dental de Soporte Implantado , Análisis de Elementos Finitos , Estrés Mecánico , Diente Premolar , Fuerza de la Mordida , Diseño de Prótesis Dental , Humanos , Mandíbula , Diente Molar
8.
Beijing Da Xue Xue Bao Yi Xue Ban ; 47(3): 521-528, 2015 Jun 18.
Artículo en Chino | MEDLINE | ID: mdl-26080887

RESUMEN

OBJECTIVE: To establish the preliminary somatosensory data stratified by gender, age group, and sites in the trigeminal region through standardized quantitative sensory testing on healthy individuals, and to evaluate the effects of gender, age, and sites on somatosensory functions. METHODS: The standardised QST battery developed by the German Research Network on Neuropathic Pain consists totally of 13 different parameters. A total of 70 healthy individuals participated. The subjects were stratified into two groups according to age: younger group (16 female, 16 male, age 24-40 years old) and elder group (20 female, 18 male, age 41-69 years old). The test was performed bilaterally over the infraorbital, mental, and hand regions. RESULTS: The preliminary orofacial somatosensory data stratified by gender, age group, and sites were presented. Female were more sensitive than male for most of the parameters (P<0.05). Age had a significant effect on most of the parameters (P<0.05), the younger group was more sensitive compared with the elder group (P<0.01) for heat pain threshold (HPT): younger group (38.07±2.94) °C, elder group (39.85±3.52) °C; warmth detection threshold (WDT): younger group (1.40±0.74) °C, elder group (1.89±1.14) °C; mechanical detection threshold (MDT): younger group (0.73±1.66) mN, elder group (1.41±2.82) mN; pressure pain threshold (PPT): younger group (171.71±92.51) kPa, elder group (196.36±73.73) kPa; cold pain threshold (CPT): younger group (25.90±5.38) °C, elder group (21.64±6.78) °C; cold detection threshold (CDT): younger group (-0.97±0.55) °C, elder group (-1.36±0.90) °C, and wind-up ratio (WUR): younger group (3.33± 2.20), elder group (2.67±1.68). The inverse results were demonstrated for mechanical pain threshold (MPT): younger group (111.50±88.93) mN, elder group (104.49±94.94) mN; mechanical pain sensitivity (MPS): younger group (6.96±5.61), elder group (8.93±6.53), and vibration detection threshold (VDT): younger group (7.44±0.52) scale, elder group (7.55±0.48) scale (P<0.05). Somatosensory function was site dependent (P<0.001), the two trigeminal sites (infraorbital and mental) were more sensitive than the hand for CDT, HPT, WDT, thermal sensory limen (TSL), MDT, MPT, MPS, and PPT (P<0.001), but the inverse result was observed for VDT (P<0.001). CONCLUSION: The preliminary orofacial somatosensory data of Han Ethnicity stratified by gender, age group, and sites were established. The study evaluated the effects of gender, age and sites on orofacial somatosensory functions by employment standardized quantitative sensory testing.


Asunto(s)
Umbral Sensorial , Adulto , Anciano , Femenino , Voluntarios Sanos , Humanos , Masculino , Persona de Mediana Edad , Dimensión del Dolor , Umbral del Dolor
9.
J Headache Pain ; 15: 71, 2014 Nov 07.
Artículo en Inglés | MEDLINE | ID: mdl-25380624

RESUMEN

BACKGROUND: Patients with migraine often have impaired somatosensory function and experience headache attacks triggered by exogenous stimulus, such as light, sound or taste. This study aimed to assess the influence of three controlled conditioning stimuli (visual, auditory and gustatory stimuli and combined stimuli) on affective state and thermal sensitivity in healthy human participants. METHODS: All participants attended four experimental sessions with visual, auditory and gustatory conditioning stimuli and combination of all stimuli, in a randomized sequence. In each session, the somatosensory sensitivity was tested in the perioral region with use of thermal stimuli with and without the conditioning stimuli. Positive and Negative Affect States (PANAS) were assessed before and after the tests. Subject based ratings of the conditioning and test stimuli in addition to skin temperature and heart rate as indicators of arousal responses were collected in real time during the tests. RESULTS: The three conditioning stimuli all induced significant increases in negative PANAS scores (paired t-test, P ≤0.016). Compared with baseline, the increases were in a near dose-dependent manner during visual and auditory conditioning stimulation. No significant effects of any single conditioning stimuli were observed on trigeminal thermal sensitivity (P ≥0.051) or arousal parameters (P ≥0.057). The effects of combined conditioning stimuli on subjective ratings (P ≤0.038) and negative affect (P = 0.011) were stronger than those of single stimuli. CONCLUSIONS: All three conditioning stimuli provided a simple way to evoke a negative affective state without physical arousal or influence on trigeminal thermal sensitivity. Multisensory conditioning had stronger effects but also failed to modulate thermal sensitivity, suggesting that so-called exogenous trigger stimuli e.g. bright light, noise, unpleasant taste in patients with migraine may require a predisposed or sensitized nervous system.


Asunto(s)
Nivel de Alerta/fisiología , Emociones/fisiología , Audición/fisiología , Umbral del Dolor/fisiología , Gusto/fisiología , Visión Binocular/fisiología , Adulto , Frío , Femenino , Frecuencia Cardíaca/fisiología , Calor , Humanos , Masculino , Trastornos Migrañosos/fisiopatología , Estimulación Física , Temperatura Cutánea/fisiología , Adulto Joven
10.
J Zhejiang Univ Sci B ; 15(4): 382-92, 2014 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-24711359

RESUMEN

OBJECTIVE: To deliver cells deep into injectable calcium phosphate cement (CPC) through alginate-chitosan (AC) microcapsules and investigate the biological behavior of the cells released from microcapsules into the CPC. METHODS: Mouse osteoblastic MC3T3-E1 cells were embedded in alginate and AC microcapsules using an electrostatic droplet generator. The two types of cell-encapsulating microcapsules were then mixed with a CPC paste. MC3T3-E1 cell viability was investigated using a Wst-8 kit, and osteogenic differentiation was demonstrated by an alkaline phosphatase (ALP) activity assay. Cell attachment in CPC was observed by an environment scanning electron microscopy. RESULTS: Both alginate and AC microcapsules were able to release the encapsulated MC3T3-E1 cells when mixed with CPC paste. The released cells attached to the setting CPC scaffolds, survived, differentiated, and formed mineralized nodules. Cells grew in the pores concomitantly created by the AC microcapsules in situ within the CPC. At Day 21, cellular ALP activity in the AC group was approximately four times that at Day 7 and exceeded that of the alginate microcapsule group (P<0.05). Pores formed by the AC microcapsules had a diameter of several hundred microns and were spherical compared with those formed by alginate microcapsules. CONCLUSIONS: AC microcapsule is a promising carrier to release seeding cells deep into an injectable CPC scaffold for bone engineering.


Asunto(s)
Cementos para Huesos , Osteoblastos/trasplante , Ingeniería de Tejidos/métodos , Células 3T3 , Alginatos , Animales , Trasplante Óseo/métodos , Calcificación Fisiológica , Fosfatos de Calcio , Cápsulas , Adhesión Celular , Diferenciación Celular , Supervivencia Celular , Quitosano , Ácido Glucurónico , Ácidos Hexurónicos , Ratones , Microscopía Electrónica de Rastreo , Osteoblastos/citología , Osteogénesis , Andamios del Tejido/química
11.
Beijing Da Xue Xue Bao Yi Xue Ban ; 46(1): 67-70, 2014 Feb 18.
Artículo en Chino | MEDLINE | ID: mdl-24535351

RESUMEN

OBJECTIVE: To evaluate the relationship of incisal point displacements in the mandibular retruded contact position (RCP) between the self-controlled retruded approach and bimanual manipulation method. METHODS: Twelve healthy young volunteers were selected. The RCP was guided through the self-controlled retruded approach and bimanual manipulation method. The track of the incisal point was recorded, using the mandibular movement trace recording system. The movement direction of the incisal point in horizontal plane was observed. The distance between the incisal point of the RCP and intercuspal contact position (ICP) was measured. RESULTS: Except one volunteer's incisal point movement direction of the RCP was oblique, others were straight toward posterior. The displacements of the incisal point from the ICP to RCP guided through self-controlled approach and bimanual manipulation method were (1.15 ± 0.64) mm, (0.98 ± 0.29) mm respectively. There was no statistical significance between the two methods (P > 0.05). CONCLUSION: The displacements of the incisal point from the ICP to RCP guided through self-controlled approach and bimanual manipulation method are approximately the same.


Asunto(s)
Oclusión Dental , Registro de la Relación Maxilomandibular , Mandíbula , Humanos , Movimiento
12.
Somatosens Mot Res ; 31(2): 62-71, 2014 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-24397569

RESUMEN

OBJECTIVES: Quantitative sensory testing has mainly used thresholds to evaluate somatosensory sensitivity so far. The variability of different measures from session to session has also been investigated, but the variability of the single individual measures of a threshold or subject-based reports has not been considered. This study aimed to investigate the potential value of threshold variability in one session as a measure of internal consistency in somatosensory function. METHODS: The standardized quantitative sensory testing battery developed by the German Research Network on Neuropathic Pain was performed bilaterally over the infraorbital, mental, and hand regions in 70 healthy and 22 temporomandibular disorder pain participants. Somatosensory variability was investigated by calculating the Coefficient of Variation of three to five repeated measures in one threshold determination. The influences of side, gender, site, age, and presence of pain on the somatosensory variability were evaluated. RESULTS: In the healthy participants, somatosensory variability was region dependent: hand > mental and/or infraorbital for CDT, WDT, HPT, MDT-N, MPT-Y, MPT-N, WUR, and MPS (p ≤ 0.043), infraorbital > hand for VDT (p = 0.001), mental > infraorbital for HPT and WUR (p ≤ 0.001); and age dependent for WDT, TSL, CPT, HPT, MDT-Y, MDT-N, MPT-N, and WUR (p ≤ 0.017). Gender and side had no main effect on variability (p ≥ 0.136). The pain patients presented higher variability compared with healthy participants for TSL, MDT-N, MPT-Y, WUR, and PPT (p ≤ 0.033). DISCUSSION: The somatosensory variability along with the threshold would be a more complete method to investigate the somatosensory disorders and underlying pain mechanisms. The correlation between pain duration and somatosensory variability should be studied further with different pain conditions.


Asunto(s)
Umbral del Dolor/fisiología , Umbral Sensorial/fisiología , Detección de Señal Psicológica/fisiología , Trastornos de la Articulación Temporomandibular/fisiopatología , Adulto , Factores de Edad , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Dimensión del Dolor , Estimulación Física , Vibración , Adulto Joven
13.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 48(8): 458-61, 2013 Aug.
Artículo en Chino | MEDLINE | ID: mdl-24238409

RESUMEN

OBJECTIVE: To measure passive tactile threshold of implant-supported single crowns (ISSC) in six orthogonal orientations, to analyze impact factors, and to guide the occlusal adjustment in a personalized manner. METHODS: The passive tactile thresholds of 24 implant-supported single crowns (5 maxillary anterior teeth, 7 maxillary posterior teeth and 12 mandibular posterior teeth) from 19 ISSC patients (8 men and 11 women, from 25 years old to 56 years old) were measured in six orthogonal orientations (four horizontal orientations of labial or buccal, lingual, mesial and distal, and two axial orientations of apical and coronal) using a digital test system for tactile function of teeth.SPSS 19.0 for windows was used to analyze impact factors, using double-sided test, with a significance level of 0.05. Paired-samples t test was used to test the difference between implant-supported single crowns and the controlled natural teeth, and between different time points. One-way ANOVA was used to test the difference between different orientations, maxilla-mandibular anterior-posterior, and men-women. RESULTS: The passive tactile thresholds of ISSC and control teeth were (1282 ± 709) and (40 ± 40) mN respectively. The difference was of statistical significance (P < 0.001). The passive tactile thresholds of buccal-lingual, mesial-distal and axial of ISSC were (1334 ± 696), (1102 ± 605) and (1412 ± 791) mN respectively, of which, the difference between mesial-distal and axial was of statistical significance (P < 0.05). The passive tactile thresholds of ISSC of maxillary anterior teeth, posterior teeth and mandibular posterior teeth were (1003 ± 616), (1302 ± 620) and (1386 ± 769) mN respectively, of which, the difference between maxillary anterior teeth and posterior teeth was of statistical significance (P < 0.05). The passive tactile thresholds of ISSC of men and women were (1751 ± 784) and (946 ± 393) mN respectively, the difference was of statistical significance (P < 0.001). The passive tactile thresholds of ISSC of one-week and one-month after wearing the teeth were (1421 ± 826) and (1411 ± 814) mN respectively, the difference was of no statistical significance (P > 0.05). CONCLUSIONS: The passive tactile threshold of ISSC was more than 65 times as much as that of the natural teeth. There was statistical significance between different orientations, between maxillary anterior and posterior teeth, and between men and women. No statistical significance was found between maxillary and madibular posterior teeth and between one-week and one-month after wearing the teeth.


Asunto(s)
Coronas , Implantes Dentales de Diente Único , Diente/fisiología , Tacto/fisiología , Adulto , Análisis de Varianza , Diente Premolar/fisiología , Diente Canino/fisiología , Prótesis Dental de Soporte Implantado , Femenino , Humanos , Incisivo/fisiología , Masculino , Mandíbula/fisiología , Maxilar/fisiología , Persona de Mediana Edad , Diente Molar/fisiología , Umbral Sensorial , Factores Sexuales
15.
J Pain ; 14(8): 793-807, 2013 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-23642433

RESUMEN

UNLABELLED: We previously developed a rat dental occlusal interference model of facial pain that was produced by bonding a crown onto the right maxillary first molar and was reflected in sustained facial hypersensitivity that was suggestive of the involvement of central sensitization mechanisms. The aim of the present study was to investigate potential central mechanisms involved in the occlusal interference-induced facial hypersensitivity. A combination of behavioral, immunohistochemical, Western blot, and electrophysiological recording procedures was used in 98 male adult Sprague Dawley rats that either received the occlusal interference or were sham-operated or naive rats. Immunohistochemically labeled astrocytes and microglia in trigeminal subnucleus caudalis (Vc) showed morphological changes indicative of astrocyte and microglial activation after the occlusal interference. Prolonged upregulation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK) was also documented in Vc after placement of the occlusal interference and was expressed in both neurons and glial cells at time points when rats showed peak mechanical facial hypersensitivity. The intrathecal administration of the p38 MAPK inhibitor SB203580 to the medulla significantly inhibited the occlusal interference-induced hypersensitivity, and the ERK inhibitor PD98059 produced an even stronger effect. Central sensitization of functionally identified Vc nociceptive neurons following placement of the occlusal interference was also documented by extracellular electrophysiological recordings, and intrathecal administration of PD98059 could reverse the neuronal central sensitization. These novel findings suggest that central mechanisms including central sensitization of trigeminal nociceptive neurons and non-neuronal processes involving MAPKs play significant roles in the production of occlusal interference-induced facial pain. PERSPECTIVE: Central mechanisms including trigeminal nociceptive neuronal sensitization, non-neuronal processes involving glial activation, and MAPKs play significant roles in occlusal interference-induced facial pain. These mechanisms may be involved in clinical manifestations of facial pain that have been reported in patients with an occlusal interference.


Asunto(s)
Sensibilización del Sistema Nervioso Central/fisiología , Oclusión Dental , Dolor Facial/enzimología , Dolor Facial/etiología , Quinasas de Proteína Quinasa Activadas por Mitógenos/metabolismo , Potenciales de Acción/efectos de los fármacos , Análisis de Varianza , Animales , Antígeno CD11b/metabolismo , Sensibilización del Sistema Nervioso Central/efectos de los fármacos , Modelos Animales de Enfermedad , Inhibidores Enzimáticos/farmacología , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Proteína Ácida Fibrilar de la Glía/metabolismo , Masculino , Neuroglía/efectos de los fármacos , Nociceptores/efectos de los fármacos , Nociceptores/fisiología , Fosfopiruvato Hidratasa/metabolismo , Ratas , Ratas Sprague-Dawley , Factores de Tiempo , Núcleos del Trigémino/metabolismo
16.
Beijing Da Xue Xue Bao Yi Xue Ban ; 45(1): 33-9, 2013 Feb 18.
Artículo en Chino | MEDLINE | ID: mdl-23411516

RESUMEN

OBJECTIVE: To evaluate the protection of calcium alginate hydrogel beads for mouse preosteoblastic cell line (MC3T3-E1) during the setting reaction of calcium phosphate cement (CPC) pastes and to analyze related influencing factors. METHODS: The MC3T3-E1 cells encapsulated in alginate hydrogel beads were cultured in vitro with ß-tricalcium phosphate/calcium phosphate cement (ß-TCP/CPC) pastes and discs until 2 d. The cell viability and number of live/dead cells were tested using the CCK-8, Calcein-AM and PI, respectively. The effects of CPC paste and CPC disc, different treatment and time to renewal medium on cell viability were compared. RESULTS: There were no significant differences in optical density and number of live cells between ß-TCP/CPC pastes and discs (1 d: P=0.827; 2 d: P=0.965). There were different influences on cell viability between different treatment and time to renewal medium. CONCLUSION: The results indicated that the calcium alginate hydrogel beads had a good protection to MC3T3-E1 cells during the setting reaction of CPC pastes. CPC paste might affect the cell proliferation even when solidification. After setting reaction, it may be favorable for cell viability to renew the medium every 6 h in 1 d.


Asunto(s)
Alginatos/farmacología , Fosfatos de Calcio/química , Supervivencia Celular/efectos de los fármacos , Osteoblastos/citología , Células 3T3 , Animales , Sustitutos de Huesos/química , Proliferación Celular/efectos de los fármacos , Ácido Glucurónico/farmacología , Ácidos Hexurónicos/farmacología , Hidrogeles/farmacología , Ratones , Microesferas , Sustancias Protectoras/farmacología
17.
Beijing Da Xue Xue Bao Yi Xue Ban ; 45(1): 50-3, 2013 Feb 18.
Artículo en Chino | MEDLINE | ID: mdl-23411519

RESUMEN

OBJECTIVE: To assess the masticatory efficiency and patient' satisfaction in long-centric occlusal pattern complete denture wearers. METHODS: The anatomic occlusal pattern and long-centric occlusal pattern complete dentures were made for each of 10 edentulous patients with severe alveolar rigde absorption simultaneously using the technique of interchangeable artificial teeth. The order of delivery of different occlusal pattern complete denture was determined according to randomized principle. For each kind of occlusal pattern complete denture, the masticatory efficiency was measured with spectrophotometer after the dentures were worn 2, 4, 6, 8, 10 and 12 weeks. A psychometric questionnaire was completed by the patients after wearing the dentures for more than three months and analyzing the patients' rating of the denture satisfaction. RESULTS: No significant differences in masticatory efficiency was found between the long-centric occlusal pattern complete denture wearers and the anatomic occlusal complete denture wearers. The patients' grade of denture satisfaction in long-centric occlusal pattern complete dentures wearers was significantly better than that in anatomic occlusal denture wearers (P<0.05). CONCLUSION: Complete denture with long-centric occlusion can be used to improve the clinical effect of edentulous patients with severe absorption of residual alveolar rigde.


Asunto(s)
Diseño de Dentadura , Dentadura Completa , Masticación/fisiología , Satisfacción del Paciente , Anciano , Anciano de 80 o más Años , Oclusión Dental Céntrica , Alisadura de la Restauración Dental , Estudios de Evaluación como Asunto , Femenino , Humanos , Masculino , Músculos Masticadores/fisiología , Boca Edéntula/fisiopatología , Proyectos Piloto
18.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 47(7): 423-6, 2012 Jul.
Artículo en Chino | MEDLINE | ID: mdl-22931574

RESUMEN

OBJECTIVE: To determine the classification of masticatory myospasm by analyzing characteristics of clinical appearances. METHODS: Thirty-six cases of masticatory myospasm from 2000 to 2010 were included. The clinical data of these patients were analyzed, including patient information, patient history, clinical characteristics, severity and the frequency of myospasmodic movement, electromyogram (EMG), and the efficacy of botulinum toxin injection treatment. RESULTS: There were 11 males and 25 females, aged from 15 to 71. According to the clinical manifestation and EMG findings, patients could be divided into two groups: 18 cases were classified as jaw closing type which involved masseter and/or temporalis muscles presenting as trismus and acute pain, the other 18 cases were jaw opening type which involved lateral pterygoid muscles complaining difficulty in jaw closing and teeth clenching. The jaw closing type was often seen in patients of 20 to 50 years old, the jaw opening was frequently seen in patients over 50 years old. Jaw closing type was attacked intermittently and unilaterally, but jaw opening was often attacked continually and bilaterally. The rating scale of the severity of spasmodic movement was not different between the two types, but the frequency of spasmodic attack was much higher for jaw opening type (P < 0.05). The EMG of jaw closing type was classified into persistent, rhythmic and irregular type. The EMG of jaw opening type was classified into spontaneous and exercise-induced type. Twelve cases were treated by botulinum toxin injection that could significantly relieve symptoms. CONCLUSIONS: Masticatory myospasm can be classified into jaw closing and jaw opening types. Jaw closing type involves masseter and/or temporalis muscles and jaw opening type involves lateral pterygoid muscles. Botulinum toxin injection was the most effective therapy for the masticatory myospasm.


Asunto(s)
Músculos Masticadores/fisiopatología , Espasmo/clasificación , Espasmo/fisiopatología , Adolescente , Adulto , Anciano , Toxinas Botulínicas Tipo A/uso terapéutico , Electromiografía , Femenino , Humanos , Maxilares/fisiopatología , Masculino , Músculo Masetero/fisiopatología , Persona de Mediana Edad , Músculos Pterigoideos/fisiopatología , Espasmo/tratamiento farmacológico , Músculo Temporal/fisiopatología , Adulto Joven
19.
Artículo en Inglés | MEDLINE | ID: mdl-22668703

RESUMEN

The available scientific literature on masticatory myospasm-induced oromandibular dystonia is limited. The objective of this study was to determine the clinical characteristics of lateral pterygoid myosapsm in a series of 18 patients. Clinical and electromyographic (EMG) data of 36 patients diagnosed with masticatory muscle myospasm were reviewed, and data from 18 patients with needle EMG-confirmed lateral pterygoid myospasm were extracted for retrospective study. The study population included 6 men and 12 women with a mean age of 53.2 ± 15.3 years. Patients' chief complaints, severity and frequency of myospasm, clinical observations, video recordings, and surface/needle EMG recordings were analyzed. Despite the variety of manifestations, 3 characteristic signs and symptoms of lateral pterygoid myospasm were observed. These were: 1) jaw function disabilities; 2) difficulty in jaw closing after wide opening; and 3) involuntary jaw movements. A differential diagnosis of lateral pterygoid myospasm should be included when oromandibular dystonia is accompanied by the fore mentioned.


Asunto(s)
Trastornos Distónicos/fisiopatología , Mandíbula/fisiopatología , Músculos Pterigoideos/fisiopatología , Espasmo/fisiopatología , Trastornos de la Articulación Temporomandibular/fisiopatología , Anciano , Discinesias/fisiopatología , Electromiografía , Femenino , Humanos , Masculino , Persona de Mediana Edad , Rango del Movimiento Articular , Estudios Retrospectivos , Índice de Severidad de la Enfermedad
20.
Beijing Da Xue Xue Bao Yi Xue Ban ; 43(1): 67-72, 2011 Feb 18.
Artículo en Chino | MEDLINE | ID: mdl-21321623

RESUMEN

OBJECTIVE: To evaluate biocompatibility of three kinds of self-developed injectable calcium phosphate cements (CPCs): chitosan microspheres/CPC, ß-tricalcium phosphate (ß-TCP)/CPC, and K(+)/CPC and the viability of the osteogenic cells cultured with CPC pastes and discs for 10 days. METHODS: The rabbit marrow stromal cells (rMSCs), isolated from rabbit bone marrow with density gradient centrifugation and flow cytometer, were cultured, expended and induced into osteoblasts. Alizarin red staining was used to determine the function of ossification. Then, rMSCs were incubated randomly on both the pastes and solidified discs of the 3 kinds of CPCs. The cells cultured on a 24-well plate were as blank control. Each group had 4 samples. The proliferation and differentiation of each group were observed using acid phosphatase assay (APA) and by testing the activity of alkaline phosphatase (ALP) at day 1, 4, 7, and 10. After stained by acridine orange(AO), the cells were observed, counted and analyzed with an epifluorescence microscopy. The morphology of the cells on CPCs was observed with scanning electron microscope(SEM).The data was subjected to two-way ANOVA followed by LSD test to compare between groups. RESULTS: The process of solidification of the three kinds of CPC pastes has the toxic effect on cells, which is irreversible. The proliferation( the average absortion of pastes are 0.049,0.050,0.049; the discs are 0.898,0.867,0.909;P<0.001), function(the average ALP activity of pastes after ten days are 0.775,0.782,0.798 U/g protein; the discs are 49.288,49.631, 49.744 U/g protein;P<0.001) and number of cells(the average number of cells of pastes after ten days are 3.7,3.7,3.7; the discs are 91.1,89.7,93.7;P<0.001) directly exposed to CPC paste significantly decreased compared with those contacting with the discs. By contrast, cells on the three kinds of discs showed better viability, proliferation, and ossification and cell numbers increased obviously with culture days. CONCLUSION: The process of solidification of the three kinds of CPC pastes has toxic effect on cells. Further study needs to explore a method to protect osteoblasts when seeded into the CPC paste.


Asunto(s)
Cementos para Huesos/farmacología , Fosfatos de Calcio/química , Osteoblastos/citología , Animales , Cementos para Huesos/química , Sustitutos de Huesos/química , Fosfatos de Calcio/farmacología , Células Cultivadas , Quitosano/química , Microesferas , Osteogénesis , Conejos
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...