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1.
J Diabetes Res ; 2020: 3838505, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32733966

RESUMEN

We have previously reported that the CNDP1 (CTG)5 allele affords protection against diabetic nephropathy (DN) in patients with Type 2 diabetes (T2DM) of Caucasian origin. Because the incidence of ESRD attributable to both Type 1 diabetes (T1DM) and T2DM is higher among South Asian than Caucasian people, the present study assessed relevant CNDP1 polymorphisms and their association with metabolic parameters in the Chinese Han population. To this end, the (CTG)n allele distribution along with 5 relevant SNPs in the CNDP1 gene, previously reported to be associated with DN in non (CTG)5 carriers of Afro-American ethnicity, were determined in 663 healthy individuals. The (CTG)6 homozygous genotype was the most prevalent (84.5%) genotype in the Chinese Han population. The (CTG)5 and (CTG)4 alleles were present in a small minority of individuals accounting for 15.2% and 0.3% of genotypes with at least one (CTG)5 or one (CTG)4 allele, respectively. Only 0.5% of individuals carried the homozygous (CTG)5 genotype and individuals carrying the homozygous (CTG)4 genotype were not found. The minor allele frequencies (MAFs) of the 5 SNP were 0.197 (C allele for rs4892247), 0.0855 (C allele for rs62099905), 0.085 (G allele for rs62099906), 0.066 (T allele for rs62099907), and 0.18 (A allele for rs72979715). All the SNPs except rs4892247 genotypes were in the Hardy-Weinberg equilibrium. Neither the (CTG)n polymorphism nor the latter three SNPs reached significance when compared with different metabolic parameters. In contrast, individuals with the TT genotype of rs62099905 presented lower fasting blood glucose but higher HbA1c levels. In conclusion, the rs62099905 in the CNDP1 gene is associated with serum glucose levels in the healthy Chinese Han population, while for the CNDP1 (CTG)n polymorphism, no association with serological parameters was found.

2.
Anal Chim Acta ; 1125: 41-49, 2020 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-32674779

RESUMEN

In clinical diagnosis of cancer, the monitoring of single tumor marker may result in many false and missed results, while simultaneous detection of multiple tumor markers should be more accuracy and effective. Here, we report a new strategy that salt-induced gold nanoparticles (AuNPs) aggregation lights up fluorescence of dual-color DNA-silver nanoclusters-aptamer (DNA-AgNCs-apta) for the simultaneous monitoring of carcinoembryonic antigen (CEA) and carbohydrate antigen 125 (CA125). The dual-color aptasensor system is composed of green-emitting DNA-AgNCs with CEA aptamer (gDNA1-AgNCs-apta1) and red-emitting DNA-AgNCs with CA125 aptamer (rDNA2-AgNCs-apta2) in the ratio of 1:1 in volume. Upon addition of AuNPs, gDNA1-AgNCs-apta1 and/or rDNA2-AgNCs-apta2 are flexibly adsorbed onto the surface of AuNPs by terminal aptamer(s), which prevents salt-induced AuNPs aggregation under high salt condition and results in fluorescence quenching based on surface plasmon enhanced energy transfer (SPEET). With the addition of CEA and/or CA125, the target(s) and corresponding aptamer(s) coordinate to form the complex, keeping DNA-AgNCs-apta(s) far away from the surface of AuNPs and making AuNPs aggregated in high salt medium. The AuNPs aggregation leads to the recovery of fluorescence signals of DNA-AgNCs-apta(s) due to weakened SPEET. Utilizing the fluorescence aptasensor system, the limit of detection of CEA and CA125 are as low as 7.5 pg·mL-1 and 0.015 U·mL-1, respectively. The proposed method can be applied to the selective and simultaneous determination of CEA and CA125 in human serum.

3.
J Cell Physiol ; 2020 Jul 11.
Artículo en Inglés | MEDLINE | ID: mdl-32654229

RESUMEN

Enolase-phosphatase 1 (ENOPH1), a newly identified enzyme involved in l-methionine biosynthesis, is associated with anxiety and depression. In this study, ENOPH1 was found to play a crucial role in promoting the proliferation and migration of glioma cells. Among high-grade glioma patients, the overall survival of the group showing high ENOPH1 expression was shorter than that of the group showing low ENOPH1 expression. ENOPH1 knockdown inhibited glioma cell proliferation and migration. In parallel, ENOPH1 knockdown suppressed tumor growth capacity and prolonged survival in an orthotopic glioma model. Mechanistically, we found that ENOPH1 activates the PI3K/AKT/mTOR signaling pathway by regulating THEM4. In conclusion, ENOPH1 is an important mediator that promotes glioma cell proliferation and migration.

4.
Aging (Albany NY) ; 122020 Jul 06.
Artículo en Inglés | MEDLINE | ID: mdl-32629427

RESUMEN

Bladder cancer is three to four times more common among men than women. The objectives of this study were to explore the association between reproductive and hormonal factors and risk of bladder cancer among women using data from the Prostate, Lung, Colorectal and Ovarian (PLCO) cohort, and to perform a meta-analysis based on cohort studies. After a median of 11.6 years of follow-up, 237 incident bladder cancer cases were identified in PLCO cohort. Compared with menopause at 50-54 years, earlier menopause (< 45 years) was positively but not significantly associated with bladder cancer risk (HR 1.25, 95% CI 0.91-1.71; p = 0.176). In the meta-analysis, parous women had significantly lower bladder cancer risk than nulliparous women (pooled HR 0.79, 95% CI 0.73-0.86). In addition, menopause at an earlier age was significantly associated with a higher risk of bladder cancer (pooled HR 1.22, 95% CI 1.06-1.40). In conclusion, this study indicated a greater risk in bladder cancer among nulliparous women and among women with early menopause. Further studies are needed to understand the underlying mechanisms.

5.
Cell Mol Neurobiol ; 2020 Jul 06.
Artículo en Inglés | MEDLINE | ID: mdl-32632892

RESUMEN

Excitatory toxicity is still a hot topic in the study of ischemic stroke, and related research has focused mainly on neurons. Adenosine is an important neuromodulator that is known as a "biosignature" in the central nervous system (CNS). The protective effect of exogenous adenosine on neurons has been confirmed, but its mechanism remains elusive. In this study, astrocytes were pretreated with adenosine, and the effects of an A2a receptor (A2aR) inhibitor (SCH58261) and A2b receptor (A2bR) inhibitor (PSB1115) on excitatory glutamate were investigated. An oxygen glucose deprivation/reoxygenation (OGD/R) and glutamate model was generated in vitro. Post-model assessment included expression levels of glutamate transporters (glt-1), gap junction protein (Cx43) and glutamate receptor (AMPAR), Na+-K+-ATPase activity, and diffusion distance of dyes. Glutamate and glutamine contents were determined at different time points. The results showed that (1) adenosine could improve the function of Na+-K+-ATPase, upregulate the expression of glt-1, and enhance the synthesis of glutamine in astrocytes. This effect was associated with A2aR activation but not with A2bR activation. (2) Adenosine could inhibit the expression of gap junction protein (Cx43) and reduce glutamate diffusion. Inhibition of A2aR attenuated adenosine inhibition of gap junction intercellular communication (GJIC) in the OGD/R model, while it enhanced adenosine inhibition of GJIC in the glutamate model, depending on the glutamate concentration. (3) Adenosine could cause AMPAR gradually entered the nucleus from the cytoplasm, thereby reducing the expression of AMPAR on the cell membrane. Taken together, the results indicate that adenosine plays a role of anti-excitatory toxicity effect in protection against neuronal death and the functional recovery of ischemic stroke mainly by targeting astrocytes, which are closely related to A2aR. The present study provided a scientific basis for adenosine prevention and ischemic stroke treatment, thereby providing a new approach for alleviating ischemic stroke.

6.
PLoS Pathog ; 16(7): e1008774, 2020 Jul 27.
Artículo en Inglés | MEDLINE | ID: mdl-32716974

RESUMEN

S-glutathionylation is an important post-translational modification (PTM) process that targets protein cysteine thiols by the addition of glutathione (GSH). This modification can prevent proteolysis caused by the excessive oxidation of protein cysteine residues under oxidative or nitrosative stress conditions. Recent studies have suggested that protein S-glutathionylation plays an essential role in the control of cell-signaling pathways by affecting the protein function in bacteria and even humans. In this study, we investigated the effects of S-glutathionylation on physiological regulation within Streptococcus mutans, the primary etiological agent of human dental caries. To determine the S-glutathionylated proteins in bacteria, the Cys reactive isobaric reagent iodoacetyl Tandem Mass Tag (iodoTMT) was used to label the S-glutathionylated Cys site, and an anti-TMT antibody-conjugated resin was used to enrich the modified peptides. Proteome profiling identified a total of 357 glutathionylated cysteine residues on 239 proteins. Functional enrichment analysis indicated that these S-glutathionylated proteins were involved in diverse important biological processes, such as pyruvate metabolism and glycolysis. Furthermore, we studied a thioredoxin-like protein (Tlp) to explore the effect of S-glutathionylation on interspecies competition between oral streptococcal biofilms. Through site mutagenesis, it was proved that glutathionylation on Cys41 residue of Tlp is crucial to protect S. mutans from oxidative stress and compete with S. sanguinis and S. gordonii. An addition rat caries model showed that the loss of S-glutathionylation attenuated the cariogenicity of S. mutans. Taken together, our study provides an insight into the S-glutathionylation of bacterial proteins and the regulation of oxidative stress resistance and interspecies competition.

7.
Huan Jing Ke Xue ; 41(3): 1377-1383, 2020 Mar 08.
Artículo en Chino | MEDLINE | ID: mdl-32608639

RESUMEN

Partial-denitrification coupled with ANAMMOX is a novel biological nitrogen removal technology, which is expected to significantly reduce the external carbon source dosage for advanced nitrogen removal from municipal wastewater. In this study, ANAMMOX sludge was inoculated to investigate advanced nitrogen removal performance and sludge characteristics in a partial-denitrification/ANAMMOX reactor. The results showed that inoculation of ANAMMOX sludge could quickly start the partial-denitrification/ANAMMOX reactor. The effluent total nitrogen concentrations were (4.82±1.84) mg·L-1 with a chemical oxygen demand of 2.19±0.08. Sludge particles larger than 0.20 mm accounted for 86.16% in the reactor. This meant that granular sludge was formed, which was conducive to good retention of ANAMMOX bacteria in the reactor. The external carbon source dosage and the oxygen requirement for nitrification can be reduced by applying partial-denitrification coupled with ANAMMOX to advanced nitrogen removal from the effluent of secondary clarifier in municipal wastewater treatment plants.

8.
Huan Jing Ke Xue ; 41(6): 2706-2713, 2020 Jun 08.
Artículo en Chino | MEDLINE | ID: mdl-32608786

RESUMEN

Two types of calcium peroxide composite tablets (CPCTs) were prepared, and the inhibition effect on the release of endogenous phosphorus and the influence on the overlying water by mixed-dosing were investigated. The CPCTs were made of calcium peroxide (CaO2), calcined water purification sludge, and hydroxypropyl methylcellulose (HPMC), which were directly pressed into composite tablets, among which Tablet-B (T-B) contained ferrous sulfate (FeSO4) and Tablet-A (T-A) did not. Both the tablets agreed well with the Langmuir and Freundlich isothermal models; the theoretical maximum adsorption capacities of T-A and T-B on phosphorus were 110.908 mg·g-1 and 106.390 mg·g-1, respectively. Compared with the control group, the pH of overlying water was increased, the concentration of Chl-a was decreased by 42.75% and 60.82%, and the DO was increased by 53.73% and 63.30% in group A and B, respectively. The DIP of the overlying water decreased significantly by 54.93% and 25.11% in group A and B, respectively. For the interstitial water in sediment, the DIP in layer Ⅰ (0-2 cm) decreased significantly by 74.81% and 65.66% in group A and B, and the DIP in layer Ⅱ (2-4 cm) decreased significantly by 46.23% in group B, but not obviously in group A. The DIP in layer Ⅲ (4-6 cm) remained unchanged in group A and B. For phosphorus fractions in sediment, the proportion of NH4Cl-P in TP was significantly increased (layer Ⅰ: 16.87% and 13.11%; layer Ⅱ: 12.99% and 11.02%, in group A and B, respectively), and the proportion of Al-P in TP was significantly decreased (layer Ⅰ: 7.58% and 13.91%; layerⅡ: 9.86% and 7.28%, in group A and B, respectively). The other phosphorus fractions did not change significantly. Both T-A and T-B dosing can improve the microbial activity of the surface sediment, though T-A can improve the microbial activity more significantly.

9.
Huan Jing Ke Xue ; 41(5): 2239-2246, 2020 May 08.
Artículo en Chino | MEDLINE | ID: mdl-32608841

RESUMEN

The occurrence and risk assessment of eight typical pharmaceutical active compounds (PhACs) in a receiving water body of wastewater treatment plant effluent were investigated. The results showed that the total concentrations of eight PhACs in summer and winter ranged from 27.6 to 226.4 ng·L-1 and 56.6 to 368.8 ng·L-1, respectively. The concentration of caffeine (16.2-125.8 ng·L-1) was highest, followed by roxithromycin (3.3-89.2 ng·L-1) and ibuprofen (3.6-59.2 ng·L-1). The total ecological risks (mixture risk quotients, MRQ) of eight PhACs to green algae, daphnia, and fish were 1.51, 0.08, and 5.68 in summer and 8.34, 0.22, and 6.45 in winter, respectively. The contribution rates of ketoconazole, erythromycin, and ibuprofen exceeded 49%, 85%, and 92% for MRQ in green algae, daphnia, and fish, respectively. In terms of sensitive species, green algae and fish are the most sensitive to PhACs in winter and summer, respectively. The results of 21 d chronic toxicity showed that mixed PhACs effect normal development and reproduction of Daphnia magna, resulting in an increase in reproductive capacity and swimming activity, and a reduction in heart rate and thoracic limb activity.

10.
Materials (Basel) ; 13(14)2020 Jul 18.
Artículo en Inglés | MEDLINE | ID: mdl-32708460

RESUMEN

This paper studies the compound effect of liquid medium and laser on the workpiece and analyses the law of material surface temperature change during the processing. Taking 7075-T6 aluminum alloy as the research object, the surface temperature field of aluminum alloy processed using water-jet-assisted laser machining under different process parameters was simulated using finite element software. In addition, the temperature field of the material surface was detected in real-time using the self-built water-jet-assisted laser machining temperature field detection system, and the processing results were observed and verified using an optical microscope, scanning electron microscope, and energy spectrum analyzer. The results show that when the water jet inflow angle is 45°, the heat-affected area of the material surface is the smallest, and the cooling effect of the temperature field of the material surface is better. Considering the liquidus melting point of 7075 aluminum alloys, it is concluded that the processing effect is better when the water jet velocity is 14 m·s-1, the laser power is 100 W, and the laser scanning speed is 1.2 mm·s-1. At this time, the quality of the tank is relatively good, there are no cracks in the bottom of the tank, and there is less slag accumulation. Compared with anhydrous laser etching, water-jet-assisted laser etching can reduce the problems of micro-cracks, molten slag, and the formation of a recast layer in laser etching and improve the quality of the workpiece, and the composition of the bottom slag does not change. This study provides theoretical guidance and application support for the selection and optimization of process parameters for water-jet-assisted laser etching of aluminum alloy and further enriches the heat transfer mechanism of multi-field coupling in the process of water-jet-assisted laser machining.

11.
Clin Chim Acta ; 510: 228-231, 2020 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-32679127

RESUMEN

BACKGROUND: Lipoprotein-associated phospholipase A2 (Lp-PLA2) is closely related to the development of cardiovascular diseases, and the association between Lp-PLA2 and lower extremity arterial disease (LEAD) in type 2 diabetes mellitus (T2DM) is inconsistent among previous studies. Thus, the present study aimed to investigate whether the increase in Lp-PLA2 is related to the occurrence of LEAD in patients with T2DM. METHODS: A total of 519 patients with T2DM (173 patients with LEAD and 346 patients without LEAD) were enrolled in this study. The demographics, medical history, serum lipids, glycosylated hemoglobin, Lp-PLA2, and ankle-brachial index (ABI) were recorded and analyzed. RESULTS: The diabetes duration, prevalence of female, prevalence of hypertension, and Lp-PLA2 concentration in the LEAD group were significantly higher than those in the non-LEAD group (duration of diabetes: 15 [10-20] vs 8 [2-12] years, prevalence of female: 49.13% vs 38.73%, prevalence of hypertension: 58.38% vs 38.11%, Lp-PLA2: 145 [108-178] vs 125 [107-138] ng/ml, p < 0.05). Lp-PLA2 was negatively correlated with ABI (r = -0.308, p < 0.001). Results of multivariate logistic regression analysis showed that serum Lp-PLA2 was an independent factor for the development of LEAD (odds ratio: 1.018 [1.007-1.029], P = 0.001). CONCLUSIONS: Increased serum Lp-PLA2 concentrations are associated with LEAD in patients with T2DM. They are an independent risk factor for the occurrence of LEAD.

12.
JAMA Netw Open ; 3(7): e208939, 2020 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-32706381

RESUMEN

Importance: Most people with commercial health insurance in the US have high-deductible plans, but the association of such plans with major health outcomes is unknown. Objective: To describe the association between enrollment in high-deductible health plans and the risk of major adverse cardiovascular outcomes. Design, Setting, and Participants: This cohort study examined matched groups before and after an insurance design change. Data were from a large national commercial (and Medicare Advantage) health insurance claims data set that included members enrolled between January 1, 2003, and December 31, 2014. The study group included 156 962 individuals with risk factors for cardiovascular disease who were continuously enrolled in low-deductible (≤$500) health plans during a baseline year followed by up to 4 years in high-deductible (≥$1000) plans with typical value-based features after an employer-mandated switch. The matched control group included 1 467 758 individuals with the same risk factors who were contemporaneously enrolled in low-deductible plans. Data were analyzed from December 2017 to March 2020. Exposures: Employer-mandated transition to a high-deductible health plan. Main Outcomes and Measures: Time to first major adverse cardiovascular event defined as myocardial infarction or stroke. Results: The study group included 156 962 individuals and the control group included 1 467 758 individuals; the mean age of members was 53 years (SD: high-deductible group, 6.7 years; control group, 6.9 years), 47% were female, and approximately 48% lived in low-income neighborhoods. First major adverse cardiovascular events among high-deductible health plan members did not differ relative to controls at follow-up vs baseline (adjusted hazard ratio, 1.00; 95% CI, 0.89-1.13). Findings were similar among subgroups with diabetes (adjusted hazard ratio, 0.93; 95% CI, 0.75-1.16) and with other cardiovascular risk factors (adjusted hazard ratio, 0.93; 95% CI, 0.81-1.07). Conclusions and Relevance: Mandated enrollment in high-deductible health plans with typical value-based features was not associated with increased risk of major adverse cardiovascular events.

13.
BMC Genomics ; 21(1): 524, 2020 Jul 29.
Artículo en Inglés | MEDLINE | ID: mdl-32727352

RESUMEN

BACKGROUNDS: Paphiopedilum is an important genus of the orchid family Orchidaceae and has high horticultural value. The wild populations are under threat of extinction because of overcollection and habitat destruction. Mature seeds of most Paphiopedilum species are difficult to germinate, which severely restricts their germplasm conservation and commercial production. The factors inhibiting germination are largely unknown. RESULTS: In this study, large amounts of non-methylated lignin accumulated during seed maturation of Paphiopedilum armeniacum (P. armeniacum), which negatively correlates with the germination rate. The transcriptome profiles of P. armeniacum seed at different development stages were compared to explore the molecular clues for non-methylated lignin synthesis. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that a large number of genes associated with phenylpropanoid biosynthesis and phenylalanine metabolism during seed maturation were differentially expressed. Several key genes in the lignin biosynthetic pathway displayed different expression patterns during the lignification process. PAL, 4CL, HCT, and CSE upregulation was associated with C and H lignin accumulation. The expression of CCoAOMT, F5H, and COMT were maintained at a low level or down-regulated to inhibit the conversion to the typical G and S lignin. Quantitative real-time RT-PCR analysis confirmed the altered expression levels of these genes in seeds and vegetative tissues. CONCLUSIONS: This work demonstrated the plasticity of natural lignin polymer assembly in seed and provided a better understanding of the molecular mechanism of seed-specific lignification process.

14.
Plant Biotechnol J ; 2020 Jul 12.
Artículo en Inglés | MEDLINE | ID: mdl-32654333

RESUMEN

Unicellular and multicellular tomato trichomes function as mechanical and chemical barriers against herbivores. Auxin treatment increased the formation of II, V and VI type trichomes in tomato leaves. The auxin response factor gene SlARF4, which was highly expressed in II, V and VI type trichomes, positively regulated the auxin-induced formation of II, V and VI type trichomes in the tomato leaves. SlARF4 overexpression plants with high densities of these trichomes exhibited tolerance to spider mites. Two R2R3 MYB genes, SlTHM1 and SlMYB52, were directly targeted and inhibited by SlARF4. SlTHM1 was specifically expressed in II and VI type trichomes and negatively regulated the auxin-induced formation of II and VI type trichomes in the tomato leaves. SlTHM1 down-regulation plants with high densities of II and VI type trichomes also showed tolerance to spider mites. SlMYB52 was specifically expressed in V type trichomes and negatively regulated the auxin-induced formation of V type trichome in the tomato leaves. The regulation of SlARF4 on the formation of II, V and VI type trichomes depended on SlTHM1 and SlMYB52, which directly targeted cyclin gene SlCycB2 and increased its expression. In conclusion, our data indicates that the R2R3 MYB-dependent auxin signalling pathway regulates the formation of II, V and VI type trichomes in tomato leaves. Our study provides an effective method for improving the tolerance of tomato to spider mites.

15.
J Comput Chem ; : e26374, 2020 Jul 02.
Artículo en Inglés | MEDLINE | ID: mdl-32618018

RESUMEN

Kinetic Monte Carlo method can provide valuable mechanistic insights for catalytic systems. Nonetheless, it suffers from the notorious problem of timescale disparity due to the existence of the complex catalytic network that consists of fast events and slow events. Previously, we have proposed the extended phenomenological kinetics (XPK) method that effectively deals with the timescale disparity problem between diffusion and reaction. However, it remains a great challenge to simulate systems with timescale disparity among different reaction pathways, which is important when selectivity is the major concern. In this study, we implement the enhanced XPK method to address this problem. The new algorithm works by identifying states connected through fast transitions and compressing them into a "superstate" when the chosen states satisfy a local steadystate condition. This state compression algorithm simplifies the reaction network by concealing the fast transitions. The accuracy and efficiency of the algorithm are demonstrated by two model systems: selective catalytic hydrogenation and selective catalytic decomposition. The enhanced XPK method is expected to be beneficial to the kinetic simulations of catalytic systems, especially those with complex reaction networks.

16.
Phys Chem Chem Phys ; 2020 Jul 20.
Artículo en Inglés | MEDLINE | ID: mdl-32685948

RESUMEN

Compared with the conventional magnetic means (such as ferromagnetic contacts), controlling a spin current by electrical methods could largely reduce the energy consumption and dimensions of nano-devices, which has become a focus of research in spintronics. Inspired by recent progress in the synthesis of an iron-based metal-organic nanostructure, we investigate the spin-dependent electronic transport of the molecule of Fe3-terpyridine-phenyl-phenyl-terpyridine-Fe3 (Fe3-TPPT-Fe3) through first-principles calculations, and propose a three-terminal device without ferromagnetics. By applying a gate voltage, not only the spin polarization can be switched between 100% and -100% to achieve a dual-spin filter, but also its fine regulation can be realized, where the transmission with any ratio of spin-up to spin-down electron numbers is achievable. Analysis shows that the particular transmission spectra are the key mechanism, where two peaks reside discretely on both sides of the Fermi level with opposite spins. Such a feature is found to be robust to the number of Fe atoms and TPPT chain length, suggesting that it is an intrinsic feature of such systems and very conducive to practical applications. The electrical control (such as an electric field) of spin polarization is realized at the single-molecule level, showing great application potential.

17.
NMR Biomed ; : e4382, 2020 Jul 20.
Artículo en Inglés | MEDLINE | ID: mdl-32686241

RESUMEN

The subthalamic nucleus (STN) and globus pallidus internus (GPi) are commonly used targets in deep-brain stimulation (DBS) surgery for the treatment of movement disorders. The success of DBS critically depends on the spatial precision of stimulation. By taking advantage of good contrast between iron-rich deep-brain nuclei and surrounding tissues, quantitative susceptibility mapping (QSM) has shown promise in differentiating the STN and GPi from the adjacent substantia nigra and globus pallidus externus, respectively. Nonlinear morphology-enabled dipole inversion (NMEDI) is a widely used QSM algorithm, but the image quality of reconstructed susceptibility maps relies on the regularization parameter selection. To date, few studies have systematically optimized the regularization parameter at the ultra-high field of 7 T. In this study, we optimized the regularization parameter in NMEDI to improve the depiction of STN and GPi at different spatial resolutions at both 3 T and 7 T. The optimized QSM images were further compared with other susceptibility-based images, including T2*-weighted (T2*w), R2*, susceptibility-weighted, and phase images. QSM showed better depiction of deep-brain nuclei with clearer boundaries compared with the other methods, and 7 T QSM at 0.35 × 0.35 × 1.0 mm3 demonstrated superior performance to the others. Our findings suggest that optimized high-resolution QSM at 7 T allows for improved delineation of deep-brain nuclei with clear and sharp borders between nuclei, which may become a promising tool for DBS nucleus preoperative localization.

18.
Gene ; 757: 144939, 2020 Jul 05.
Artículo en Inglés | MEDLINE | ID: mdl-32640306

RESUMEN

Osteoarthritis (OA) is a chronic degenerative change with high incidence and leads to a lower quality of life and a larger socioeconomic burden. This study aimed to explore potential crucial genes and pathways associated with OA that can be used as potential biomarkers forearly treatment. Single-cell gene expression profile of 1464 chondrocytes and 192 fibroblasts in OA were downloaded from the public database (GSE104782 and GSE109449) for subsequent analysis. A total of eight clusters in chondrocytes and three clusters in fibroblasts of OA were identified using the Seurat pipeline and the "SingleR" package for cell-type annotation. Moreover, 44 common marker-genes between fibroblastic-like chondrocytes and fibroblasts were identified and the focal adhesions pathway was further identified as a significant potential mechanism of OA via functional enrichment analysis. Further, the reverse transcription quantitative real-time PCR (RT-qPCR) experiments at tissue's and cellular level confirmed that two key marker-genes (COL6A3 and ACTG1) might participate in the progression of OA. Summarily, we inferred that chondrocytes in OA might up-regulate the expression of COL6A3 and ACTG1 to complete fibroblasts transformation through the focal adhesion pathway. These findings are expected to gain a further insight into the development of OA fibrosis process and provide a promising target for treatment for early OA.

19.
Sci Rep ; 10(1): 11740, 2020 Jul 16.
Artículo en Inglés | MEDLINE | ID: mdl-32678169

RESUMEN

The distribution of rare earth elements and the microbial community in nearby ground water and soil were influenced by tailings ponds. Accordingly, the behaviors of rare earth elements in ground water and soil around the tailings pond, and the changes of microbial communities were both investigated in this study. The results showed that rare earth elements accumulated in ground water and soil around the tailings pond appeared as light rare earth elements enrichment. Through the normalization of rare earth elements, different extents of anomaly (from negative to positive) were observed for Ce and Eu in the distribution patterns of REEs in groundwater, however, Ce and Eu were negatively anomaly in soil. According to the correlation analysis, Mn2+, SO42-, Cl-, ammonia nitrogen and Ca2+ are significantly correlated with the distribution of rare earth elements. Meanwhile, there were the same dominant bacteria in ground water and soil including Actinobateria, Proteobacteria and Acidobacteria at the phylum level. This microbial community composition is similar to that reported in arid lands around the world. On the other hand, Bacillus and Blastococcus showed significant correlation with rare earth elements at the genus level. This study might provide an important basis for the risk assessment of REEs in the environment.

20.
Biomed Res Int ; 2020: 3945189, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32695813

RESUMEN

Oral cavity has been taken as one of the major reservoirs for Helicobacter pylori, the bacteria responsible for gastric infection and cancers. Dentists are frequently exposed to saliva; thus, theoretically, they are at a higher risk for oral H. pylori infection. In the present study, to test this hypothesis and to find out the potential factors associated with the increased risk, a cross-sectional study was carried out on a large scale of dentists (N = 90) and nondentist controls (N = 110). By using nested polymerase chain reaction to amplify a specific DNA fragment of H. pylori, we found 7.27% of saliva samples from the nondentist group and 16.67% of saliva samples from the dentist group were oral H. pylori positive, and the difference between groups was statistically significant (χ 2 = 4.292, p = 0.038). Importantly, however, after stratifying enrolled subjects with factors which might interfere with the comparison of H. pylori detection rate between groups, we still observed a higher H. pylori frequency in the dentists than that in the controls in subgroups, including those with good individual hygiene, healthy lifestyle, and physical condition, as well as those living with families to be gastric disease free and not sharing meals with H. pylori-positive persons, respectively. Moreover, the frequency of clinical practice per week of the investigated dentists was closely associated with an oral H. pylori infection risk. Our data indicates that dentists are at a higher risk for H. pylori infection, and intensive attention needs to be paid on this issue.

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