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1.
Talanta ; 207: 120260, 2020 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-31594598

RESUMEN

Metabolomics tactics have been applied in the research associated with embryonic zebrafish. However, the report regarding the evaluation of impacts of sample pretreatment on metabolomics results from zebrafish embryos is limited. In the present study, different data normalization approaches, extraction solvents, and extraction strategies for off-line derivatization gas chromatography coupled with mass spectrometry-based metabolomics analysis of zebrafish eleutheroembryos were evaluated and optimized. The results showed that, when 4-chlorophenylalanine normalization, sample homogenization and pure methanol combined with ultrasonic extraction were conducted, better repeatabilities, higher signals and broader coverages of detected metabolites can be achieved. The recovery and standard deviation of most standards were in the range of 82%-121% and 6.6%-12%, respectively, while the relative standard deviation of major detected metabolites ranged from 5.4% to 19%, indicating good extraction efficiencies and method precision. Under the developed method, 87 important endogenous metabolites such as citric acid and hypoxanthine were identified by universal databases or standards among 270 extracted metabolites, which consisted of sugars, amines, amino acids, nucleotides, fatty acids, and sterols. Therefore, the results could provide a proper pretreatment protocol for the analysis of wide-coverage metabolome in embryonic zebrafish. In addition, this study highlights the impact of normalization and extraction methods on the data quality of metabolomics analysis.

2.
J Glaucoma ; 2019 Sep 25.
Artículo en Inglés | MEDLINE | ID: mdl-31567910

RESUMEN

PRéCIS:: Chinese Glaucoma Study Consortium (CGSC) as the first nation-wide glaucoma registry in China, we describe its design, rationale, the geographic distribution of the hospitals, and baseline patient characteristics. AIM: As a leading cause of blindness in China, glaucoma affects 2-4% of adults over the age of 40 and will become increasingly prevalent as the population ages. At the national level, there are few reports on the current medical practice for glaucoma patients. Chinese Glaucoma Study Consortium (CGSC) will be considered as the first nation-wide glaucoma registry in China. Here we describe its design, rationale, the geographic distribution of the hospitals, and baseline patient characteristics. METHODS: From December 21st 2015 to September 9th 2018, CGSC recruited patients with the diagnoses of primary angle-closure suspect (PACS), primary angle-closure (PAC), primary angle-closure glaucoma (PACG), acute primary angle closure (APAC), primary open-angle glaucoma (POAG), pigmentary glaucoma (PG) and pseudoexfoliative glaucoma (PXG) from 111 hospitals covering 67 cities from 22 provinces, 4 municipalities, and 5 autonomous regions in mainland China. Clinical data were collected using an Electronic Data Capture System designed by Tongren hospital and Gauss informed Ltd. Blood samples were collected from every patient for further genetic analysis. RESULTS: Medical records of 10892 patients were collected, of which 5762 patients have complete information. The average age of those with complete information (n=5762) was 62.05±11.26 years old, and 35.25% were males. Primary angle-closure disease (PACD), including PACS\PAC\PACG\APAC, was predominant (4588, 79.63%), and the distribution for others is as follows: primary open-angle glaucoma (1116, 19.37%), pseudoexfoliative glaucoma (41, 0.71%) and pigmentary glaucoma (17, 0.30%). A total of 16684 blood samples were collected, of which 9917 (82.68%) were PACD, 1987 (16.57%) were POAG, 69 (0.58%) were PXG, 22 (0.18%) were PG, and 4689 were normal controls. CONCLUSIONS: The CGSC is the first national-level glaucoma registry study in China. Clinical data and blood samples will provide the opportunity to study the epidemiology of glaucoma in Chinese hospitals, to evaluate the level of medical diagnosis and treatment of glaucoma in China, and to identify the susceptibility loci for glaucoma.

3.
Medicine (Baltimore) ; 98(39): e17124, 2019 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-31574812

RESUMEN

INTRODUCTION: Spasticity is the most common complication after stroke, which is the main obstacle in the recovery of motor function. Spasticity seriously affects the quality of life and brings a heavy burden to families and society. Acupuncture is an effective method for stroke. However, whether acupuncture is effective for poststroke spasticity is still unknown. The purpose of this systematic review (SR) is to evaluate the effectiveness and safety of acupuncture for poststroke spasticity. METHODS AND ANALYSIS: We will search the following databases from inception to July 2019: China Biology Medicine (CBM), China National Knowledge Infrastructure (CNKI), Wan Fang Data, the Chinese Science and Technology Periodical Database (VIP), PubMed, Embase, The Cochrane Library, and Web of Science. All relevant randomized controlled trials (RCTs) utilizing acupuncture for poststroke spasticity will be included. The primary outcome is the modified Ashworth scale. Secondary outcomes include composite spasticity scale, clinic spasticity index, electromyographic activity, Hoffmann reflex activity, or other spasticity-related outcomes. Study selection, data extraction, and quality assessment will be performed independently by 2 reviewers. Assessment of risk of bias and data synthesis will be conducted using Review Manager V5.3 software. ETHICS AND DISSEMINATION: The ethical approval is not required since SR is based on published studies. The results of this SR will be published in a peer-reviewed scientific journal according to the Preferred Reporting Item for Systematic Review and Meta-analysis (PRISMA) guidelines. PROSPERO REGISTRATION NUMBER: CRD42019129779.

4.
Drug Des Devel Ther ; 13: 3009-3019, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31564827

RESUMEN

Objective: The aim of this study was to identify the active anti-ischemic components of Pterocypsela elata (P. elata) using a network pharmacology approach to construct an effective component anti-cerebral ischemic target network and systematically analyze this medicinal material. Methods: Pharmacological studies have shown that P. elata has an obvious effect against cerebral ischemia. To identify the potential targets, 14 components of P. elata were docked to each structural element of the targets in the DRAR-CPI database by reverse docking technology. We then compared the identified potential targets with FDA-approved targets for stroke/cerebral infarction treatment in the DrugBank database and identified the active components of P. elata and their potential targets for stroke/cerebral infarction treatment. The active component-target networks were constructed using Cytoscape 3.5.1 software. The target protein-protein interactions were analyzed using the STRING database. KEGG pathway analysis and gene ontology (GO) enrichment analysis were performed through the Database for Annotation, Visualization and Integrated Discovery (DAVID). Results: There were 14 active components identified from P. elata and 21 potential targets identified for cerebral ischemia treatment, including carbonic anhydrase 2, ribosyldihydronicotinamide dehydrogenase, cholinesterase, and glutathione S-transferase P. The main involved pathways include metabolic pathways, complement and coagulation cascades and steroid hormone biosynthesis. Conclusion: Through a network pharmacology approach, we predicted the active components of P. elata and their potential targets for cerebral ischemia treatment. Our results provide new perspectives and clues for further studies on the anti-cerebral ischemia mechanism of P. elata.

5.
Int J Chron Obstruct Pulmon Dis ; 14: 2081-2088, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31564855

RESUMEN

Background: Chronic obstructive pulmonary disease (COPD) is a progressive lung disease characterized by incomplete reversible airflow limitation, which is associated with emphysema and chronic inflammation. Oxidative/antioxidant imbalance is one of the mechanisms of the current pathogenesis of COPD and several recent studies have attempted to uncover genetic causes of COPD and its progression. GST, HO-1, and SOD-3 are important susceptibility genes related to COPD. Methods: A total of 300 blood samples were included in two groups: Control group and COPD group. We genotyped 4 single nucleotide polymorphisms (SNPs) from these 3 genes in 150 COPD patients and 150 controls to analyze genetic polymorphisms and interactions with COPD-related quantitative traits using correlation analysis and multivariate logistic regression analysis. Results: The results indicated that genotype distributions and allele frequencies of GSTP1, HO-1, and SOD-3 were significantly different between the COPD and the control group, while there is no correlation between the polymorphism of GSTP1, HO-1, SOD3, and the different stages of COPD. Furthermore, multivariate logistic regression analysis indicated that COPD GSTP1-exon5 SNP and HO-1 (GT)n SNP are high-risk factors for COPD and there was interaction between GSTP1 exon5 SNPS and HO-1 (GT)n SNP. More important, the genotypes, AG, GG of GSTP1 exon5 and L/M*S, L/L of HO-1 (GT)n associated with increased 8-iso-prostaglandin F (2 alpha) (8-iso-PGF2) and malondialdehyde (MDA) concentration and decreased catalase (CAT) activity. Conclusion: Collectively, this study shows that genetic polymorphisms of GSTP1, HO-1, and SOD-3 are associated with COPD susceptibility.

6.
Artículo en Inglés | MEDLINE | ID: mdl-31566428

RESUMEN

The transient receptor potential canonical 6 (TRPC6) channel and podocin are co-localized in the glomerular slit diaphragm as an important complex to maintain podocyte function. Gain of TRPC6 function and loss of podocin function induce podocyte injury. We have previously shown that high glucose induces apoptosis of podocytes by activating TRPC6; however, whether the activated TRPC6 can alter podocin expression remains unknown. Western blot, confocal microscopy, and scanning ion conductance microscopy were used to examine both the expression levels of TRPC6, podocin, and nephrin and the morphological change of podocytes in response to high glucose. High glucose increased the expression of TRPC6, but reduced the expression of podocin and nephrin, both in cultured human podocytes and in type 1 diabetic rat kidney. The decreased podocin was diminished in TRPC6 knockdown podocytes. High glucose elevated intracellular calcium in control podocytes, but not in TRPC6 knockdown podocytes. High glucose also elevated the expression of a tight junction protein, zonula occludens-1 (ZO-1) and induced the redistribution of ZO-1 and the loss of podocyte processes. These data together suggest that high glucose reduces the protein levels of podocin by activating TRPC6 and induces morphological changes of cultured podocytes.

7.
Eur J Pharmacol ; 863: 172701, 2019 Sep 27.
Artículo en Inglés | MEDLINE | ID: mdl-31568784

RESUMEN

Myocardial ischemia/reperfusion (IR) injury is caused by the restoration of the coronary blood flow following an ischemic episode. Accumulating evidence suggests that galectin-3, a ß-galactoside-binding lectin, acts as a biomarker in heart disease. However, it remains unclear whether manipulating galectin-3 affects the susceptibility of the heart to IR injury. In this study, RNA sequencing (RNA-seq) analysis identified that Lgals3 (galecin-3) plays an indispensable role in IR-induced cardiac damage. Immunostaining and immunoblot assays confirmed that the expression of galectin-3 was markedly increased in myocardial IR injury both in vivo and in vitro. Echocardiographic analysis showed that cardiac dysfunction in experimental IR injury was significantly attenuated by galectin-3 inhibitors including pectin (1%, i.p.) from citrus and binding peptide G3-C12 (5.0 mg/kg, i.p.). Galectin-3 inhibitor-treated mice exhibited smaller infarct sizes and decreased tissue injury. Furthermore, TUNEL staining showed that galectin-3 inhibition suppressed IR-mediated cardiomyocyte apoptosis. Mitochondrial membrane potential (MMP) and mitochondrial permeability transition pore (mPTP) levels were well-preserved and IR-induced changes of mitochondrial cyto c, cytosol cyto c, caspase-9, caspase-3, Bcl-2 and Bax in the galectin-3 inhibitor-treated groups were observed. Our findings indicate that the pathological upregulation of galectin-3 contributes to IR-induced cardiac dysfunction and that galectin-3 inhibition ameliorates myocardial injury, highlighting its therapeutic potential.

8.
Med Sci Monit ; 25: 7202-7208, 2019 Sep 25.
Artículo en Inglés | MEDLINE | ID: mdl-31587013

RESUMEN

BACKGROUND Hypoxia promotes cancer progression. Hypoxia-inducible factor-1alpha (HIF-1alpha) has been reported to enhance tumor invasion and metastasis via activating downstream genes, such as matrix metalloproteinases (MMPs). The purpose of this study was to explore the probable roles of HIF-1alpha and MMP13 in the invasion and metastasis of ovarian cancer under hypoxic conditions. MATERIAL AND METHODS The expression of HIF-1alpha and MMP13 protein were detected with immunohistochemistry staining in ovarian cancer tissues, metastatic lesions, and normal fallopian tissues. Ovarian cancer A2780 cells were cultured under normoxic condition and hypoxic condition. mRNA and protein expression of HIF-1alpha and MMP13 were detected by RT-PCR and Western blot analysis. The effects of siRNA against HIF-1alpha on MMP13 expression were examined by RT-PCR and Western blot analysis. Transwell invasion assays were performed to test the invasive ability of A2780 cells. RESULTS Immunohistochemistry staining showed significantly higher expression of HIF-1alpha and MMP13 protein in ovarian cancer tissues and metastatic lesions than in normal fallopian tissues. HIF-1alpha and MMP13 expression were closely related. After exposure to hypoxia, mRNA and protein levels of HIF-1alpha and MMP13 were upregulated. siRNA effectively inhibited HIF-1alpha expression and MMP13 expression. The number of invading A2780 cells decreased after HIF-1alpha was silenced. CONCLUSIONS This study suggests that HIF-1alpha promotes ovarian cancer cell invasion through a MMP13 mechanism. It might be an effective strategy targeting HIF-1alpha - MMP13 to inhibit invasion and metastasis of ovarian cancer.

9.
J Exp Bot ; 2019 Oct 06.
Artículo en Inglés | MEDLINE | ID: mdl-31587068

RESUMEN

Transfer cells (TCs) are characterized by an amplified plasma membrane area supported on a wall labyrinth composed of a uniform wall layer (UWL) from which wall ingrowth (WI) papillae arise. Adaxial epidermal cells of developing Vicia faba cotyledons, when placed in culture, undergo a rapid (hour) trans-differentiation to a functional epidermal TC (ETC) phenotype. The trans-differentiation event is controlled by a signalling cascade comprising auxin, ethylene, apoplasmic reactive oxygen species (apoROS) and cytosolic Ca2+. Apoplasmic hydrogen peroxide (apoH2O2) was confirmed as the apoROS regulating UWL and WI papillae formation. Informed by an ETC-specific transcriptome, a pharmacological approach identified a temporally changing cohort of H2O2 biosynthetic enzymes. The cohort contained a respiratory burst oxidase homologue, polyamine oxidase, copper amine oxidase and a suite of class III peroxidases. Collectively these generated two consecutive bursts in apoH2O2 production. Spatial organization of biosynthetic/catalytic enzymes was deduced from responses to pharmacologically blocking their activities on the cellular and sub-cellular distribution of apoH2O2. The findings were consistent with catalase activity constraining the apoH2O2 signal to the outer periclinal wall of the ETCs. Strategic positioning of class III peroxidases in this outer domain shaped sub-cellular apoH2O2 signatures that differed during assembly of the UWL and WI papillae.

10.
Artículo en Inglés | MEDLINE | ID: mdl-31577118

RESUMEN

Inspired by the collective behavior of natural living systems, the collective behavior of micromotors has become the research highlight. Although great progresses have been made, it is still challenging to control the collective behavior of micromotors. In this paper, we demonstrate a novel near infrared (NIR) light-powered micromotor consisting of polystyrene microsphere and polydopamine core-shell structure (PS@PDA) with concentration-dependent motion direction reversal and dynamic swarming behavior. Among others, single micromotor exhibits the negative phototaxis while a group of micromotors show the positive phototaxis, which can be attributed to the competition between the thermophoretic force and hydrodynamic drag caused by the thermal buoyancy. In addition, due to the reversible hydrogen bonding and π-π stacking interactions between the adjacent PS@PDA micromotors, they form aggregation as a result of the positive phototaxis with dynamically controllable shapes tuned by the irradiation position, which makes them potentially attractive for in-solution calligraphy and painting. It is anticipated that the current study may not only provide a new strategy to control the collective behavior of the micromotors, but also promote their application in the practical field.

11.
Medicine (Baltimore) ; 98(40): e17126, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31577702

RESUMEN

BACKGROUND: The aim of this study was to investigate the role of n-acetyl cysteine (NAC) in the lipopolysaccharide (LPS)-mediated induction of tumor necrosis factor-α (TNF-α) and interleukin-1ß (IL-1ß) synthesis by human periodontal ligament fibroblast cells (hPDLFs). In addition, we aimed to determine the involvement of the nuclear factor-kappa B (NF-κB) pathway in any changes in IL-1ß and TNF-α expression observed in response to LPS and NAC. METHODS: HPDLFs were obtained by primary culture. The culture medium used in this experiment was Dulbecco's Modified Eagle Medium (DMEM low-glucose). Cells were stimulated with various concentrations of NAC or LPS. Cell proliferation was measured at various time-points with the cell Counting Kit 8 (CCK-8) assay. mRNA levels of IL-1ß and TNF-α were determined by real-time quantitative polymerase chain reaction (RT-qPCR) analysis. Protein levels of IL-1ß and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA). Protein and mRNA expression levels of NF-κB were measured by western blot and RT-qPCR. RESULTS: The results showed that LPS treatment in hPDLFs induced mRNA and protein expression of IL-1ß, TNF-α, and NF-κB. However, these effects were eliminated by pretreatment with NAC. Pretreatment with both NAC (1 mmol/L) and BAY11-7082 (10 µmol/L) significantly inhibited the NF-κB activity induced by LPS. CONCLUSION: NAC inhibits the LPS-mediated synthesis of tumor TNF-α and IL-1ß in hPDLFs, through the NF-κB pathway.

12.
Clin Cancer Res ; 2019 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-31573954

RESUMEN

PURPOSE: Treatment outcomes for childhood acute lymphoblastic leukemia (ALL) have improved steadily, but a significant proportion of patients still experience relapse due to drug resistance, which is partly explained by inherited and/or somatic genetic alternations. Recently, we and others have identified genetic variants in the ARID5B gene associated with susceptibility to ALL and also with relapse. In this study, we sought to characterize the molecular pathway by which ARID5B affects antileukemic drug response in patients with ALL. EXPERIMENTAL DESIGN: We analyzed association of ARID5B expression in primary human ALL blasts with molecular subtypes and treatment outcome. Subsequent mechanistic studies were performed in ALL cell lines by manipulating ARID5B expression isogenically in which we evaluated drug sensitivity, metabolism, and molecular signaling events. RESULTS: ARID5B expression varied substantially by ALL subtype, with the highest level being observed in hyperdiploid ALL. Lower ARID5B expression at diagnosis was associated with the risk of ALL relapse, and further reduction was noted at ALL relapse. In isogenic ALL cell models in vitro, ARID5B knockdown led to resistance specific to antimetabolite drugs (i.e., mercaptopurine and methotrexate), without affecting sensitivity to other antileukemic agents. ARID5B downregulation significantly inhibited ALL cell proliferation and caused partial cell-cycle arrest. At the molecular level, the cell-cycle checkpoint regulator p21 (encoded by CDKN1A) was most consistently modulated by ARID5B, plausibly as its direct transcription regulation target. CONCLUSIONS: Our data indicates that ARID5B is an important molecular determinant of antimetabolite drug sensitivity in ALL, in part through p21-mediated effects on cell-cycle progression.

13.
Carbohydr Polym ; 226: 115279, 2019 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-31582078

RESUMEN

Hydrogels with low-adhesive superoleophobicity are ideal candidates for modifying filtration substrates to achieve efficient and antifouling oil/water separation. However, there are still some unfavorable factors hindering their practical application, including expensive raw materials, complex fabrication process, poor stability and durability. In this work, a durable, cost-effective and superhydrophilic chitosan-alginate (CS-ALG) hydrogel coated mesh was developed by a facile, two-step dip-coating method for efficient oil/water separation in hypersaline environments. By integrating polysaccharide-based superhydrophobic surfaces and the hierarchical micro-/nanostructures, the as-fabricated CS-ALG hydrogel coated mesh exhibits excellent underwater superoleophobicity and anti-oil-fouling performance. Benefiting from that, the mesh could separate various oil/water mixtures with high separation efficiency (> 99%). It is worth mentioning that the double-cross-linked CS-ALG hydrogel based on sequential electrostatic interaction and ionic cross-linking shows excellent durability in hypersaline environments. All these attractive advantages make the hydrogel-coated mesh a promising candidate for oily wastewater treatment and oil spill cleanup.

14.
Chem Pharm Bull (Tokyo) ; 67(10): 1088-1098, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31582628

RESUMEN

In this study, we synthesized four series of novel L-homoserine lactone analogs and evaluated their in vitro quorum sensing (QS) inhibitory activity against two biomonitor strains, Chromobacterium violaceum CV026 and Pseudomonas aeruginosa PAO1. Studies of the structure-activity relationships of the set of L-homoserine lactone analogs indicated that phenylurea-containing N-dithiocarbamated homoserine lactones are more potent than (Z)-4-bromo-5-(bromomethylene)-2(5H)-furanone (C30), a positive control for biofilm formation. In particular, compared with C30, QS inhibitor 11f significantly reduced the production of virulence factors (pyocyanin, elastase and rhamnolipid), swarming motility, the formation of biofilm and the mRNA level of QS-related genes regulated by the QS system of PAO1. These results reveal 11f as a potential lead compound for developing novel antibacterial quorum sensing inhibitors.

15.
Retina ; 2019 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-31584558

RESUMEN

PURPOSE: To determine the ability of nonperfusion, vessel density, and morphologic measurements using projection-resolved optical coherence tomography angiography to detect early retinal microvasculature impairments in diabetes mellitus. METHODS: A retrospective review was performed on Type 2 diabetes mellitus patients with no diabetic retinopathy (DR) or mild nonproliferative DR and age-matched controls imaged with optical coherence tomography angiography. Foveal avascular zone-related metrics and extrafoveal avascular area were measured in optical coherence tomography angiography images. Vessel density and fractal dimension were calculated with and without a skeletonization process. The vessel diameter index and vessel tortuosity were computed. The area under the receiver operating characteristic curve (AUC) estimated diagnostic performances. RESULTS: Dilated capillary diameter was observed in the deep capillary plexus in the diabetic groups. Vessel density and fractal dimension of skeletonized deep capillary plexus significantly and progressively decreased in the no DR and mild nonproliferative DR groups compared with controls. Superficial extrafoveal avascular area, vessel density, and fractal dimension of the skeletonized deep capillary plexus had the highest diagnostic performance to differentiate mild nonproliferative DR from control eyes, with AUCs of 0.885, 0.876, and 0.876, respectively. CONCLUSION: Vessel density and fractal dimension from the skeletonized deep capillary network may be the most sensitive for detecting early retinal capillary loss in diabetes mellitus.

16.
J Agric Food Chem ; 2019 Oct 07.
Artículo en Inglés | MEDLINE | ID: mdl-31589432

RESUMEN

Peanut is an important legume providing products with nutrient dense including mineral nutrition. However, the genetic basis underlying the variations in the mineral composition in peanut is still unknown. A genome-wide association studies (GWAS) of the concentrations of 13 mineral elements combine with co-expression network were performed using a diverse panel of 120 genotypes mainly selected from the U.S. peanut mini core collection. A total of 36 significant quantitative trait loci (QTLs) associated with 5 elemental concentrations were identified with phenotypic variation explained (PVE) from 18.35% to 27.56%, in which 24 QTLs are for boron (B), 2 QTLs for copper (Cu), 6 QTLs for sodium (Na), 3 QTLs for sulfur (S), and 1 QTL for zinc (Zn). A total of 110 non-redundant candidate causal genes identified were significantly associated with elements accumulation, which include one high-priority overlap (HPO) candidate gene arahy.KQD4NT, an important elemental/metal transporter gene located on LGB04 with position 5,413,913-5,417,353.

17.
Environ Pollut ; 255(Pt 2): 113267, 2019 Sep 25.
Artículo en Inglés | MEDLINE | ID: mdl-31574391

RESUMEN

In recent years, ozone pollution has become more and more serious in China. Several epidemiological studies have demonstrated the correlation between short-term ozone exposure and several health risks including all-cause mortality, cardiovascular mortality, and respiratory mortality. In this study, the daily ozone exposure levels with 10 km × 10 km resolution were estimated based on satellite data derived from Ozone Monitoring Instrument (OMI) and the monitoring data. The health impacts for potential decrease in the daily ozone concentration and the corresponding economic benefits in 2016 were estimated by applying the environmental Benefits Mapping and Analysis Program-Community Edition (BenMAP-CE) model. By reducing the daily maximum 8-h average concentration of ozone to 100 µg/m3, the estimated avoided all-cause mortalities were 120 × 103 (95% confidence interval (CI): 67 × 103, 160 × 103) cases and the correspondingly economic benefits ranged from 36 to 64 billion CNY using amended human capital (AHC) and willingness to pay (WTP) method in 2016. If the daily maximum 8-h average concentration of ozone were rolled back to 70 µg/m3, the estimated avoided all-cause mortalities were 160 × 103 (95% CI: 98 × 103, 230 × 103) cases and economic benefits ranged from 54 to 95 billion CNY based on AHC and WTP methods.

18.
J Agric Food Chem ; 2019 Oct 10.
Artículo en Inglés | MEDLINE | ID: mdl-31600056

RESUMEN

Sublethal exposure to neonicotinoids affects honey bee olfaction, but few studies have investigated the sublethal effects of the enantioselective neonicotinoid dinotefuran on honey bee olfaction. This study assessed the sublethal olfactory toxicity of dinotefuran enantiomers to honey bees. Compared to R-dinotefuran, S-dinotefuran had higher acute oral toxicity, sucrose sensitivity effects and octopamine concentrations and lower learning ability and memory effects on honey bees. High-throughput circular RNA sequencing of the honey bee brain revealed that R-dinotefuran caused more gene regulatory changes than S-dinotefuran. Gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses demonstrated that the SERCA, Kca and Maxik genes may be related to the enantioselective effects of dinotefuran isomers on honey bee olfaction. These results indicated that the current ecotoxicological safety knowledge about chiral dinotefuran effectcs on honey bees should be amended.

19.
Acta Trop ; : 105212, 2019 Oct 07.
Artículo en Inglés | MEDLINE | ID: mdl-31600521

RESUMEN

Fasciolosis is one of the biggest threats to livestock and human population. For this purpose, the seroprevalence of Fasciola hepatica was investigated in yaks and sheep living on the Qinghai-Tibet plateau, China by piloting commercial ELISA kits. A total of 3276 yaks and 1092 sheep were incorporated in this study. The prevalence of the parasite in yaks and sheep was 38.3% and 26.4%, respectively. The serological results revealed a relatively high prevalence of F. hepatica infection in yaks and sheep, respectively. The present study may greatly contribute to the prevention of this parasitic zoonosis and great importance should be given to the potential threat caused by F. hepatica in this special plateau.

20.
Zhongguo Zhong Yao Za Zhi ; 44(15): 3239-3245, 2019 Aug.
Artículo en Chino | MEDLINE | ID: mdl-31602878

RESUMEN

The present study was conducted to explores the effects of short-term addition of 17ß-E2 on the growth,gonad development and internal quality of overwintering Whitmania pigra. Before overwintering,0. 0,1. 0,10. 0,25. 0,50. 0,100. 0 µg·L~(-1) of 17ß-E2 were added to the aquaculture water for 6 weeks and then hibernated for 60 days. The changes of growth performance,gonad index,morphological structure of spermary( ovary),endogenous steroid hormones level and internal quality were measured. The results showed that the body weight,weight gain rate,specific growth rate,female gonad index,oocyte development and endogenous estrogen level of the leech increased first and then decreased with the increase of the concentration of exogenous 17ß-E2,which were higher than those of the control group. The body weight,weight gain rate and specific growth rate of the leech at the concentration of 25 µg·L~(-1)17ß-E2 were significantly higher than those of the other groups( P<0. 05),oocyte development and endogenous estrogen levels were significantly higher than those of other groups at the concentration of 50 µg·L~(-1)( P<0. 05). When the concentration of exogenous 17ß-E2 was higher than 50 µg·L~(-1),the levels of male gonad index,spermatocyte development,endogenous androgen and progesterone were significantly inhibited( P< 0. 05). There was no significant difference in endogenous corticosteroid levels among the groups. In conclusion,short-term addition of exogenous 17ß-E2 of 10-25 µg·L~(-1) could promote the growth of overwintering leeches,oocyte development and antithrombin activity without inhibiting the development of male gonads.

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