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1.
RSC Adv ; 12(49): 32119-32128, 2022 Nov 03.
Artículo en Inglés | MEDLINE | ID: mdl-36415554

RESUMEN

Seven new metal coordination complexes, [NiC15H43N5O11] (I), [Co3C36H98N6O6] (II), [CuC14H32N2O6] (III), [Cu2C32H43Cl2N2O13] (IV), [Zn2C24H32Cl3N3O3] (V), [Co3C48H66Cl6N6O6] (VI), and [Zn (C18H45N3O3] (VII), have been synthesized from some direct reactions of amino-alcoholic ligands with metal salts in anhydrous methanol or ethanol medium. All the crystals of these seven complexes are crystallized in the chiral space groups (P212121 for (I), (IV), (VI) and (VII); P21 for (III) and (V); and C2 for (II), respectively). Their characteristic peaks were analyzed and assigned by FTIR, NMR, and UV-Vis and elemental analysis techniques. The anticancer activities of amino alcohol complexes (I)-(VII) showed cytotoxic effects against the human tumour cell line A549; among them, complex (V) showed the best activity with an IC50 value of 17.8. The higher biological activity should be related to its di-nuclear zinc(ii) unit in which one zinc is only four-coordinated by four small chloride anions.

2.
Zhonghua Nei Ke Za Zhi ; 61(11): 1228-1233, 2022 Nov 01.
Artículo en Chino | MEDLINE | ID: mdl-36323564

RESUMEN

Objective: To investigate the expression of Macrophage migration-inhibitory factors (MIF) in hepatocellular carcinoma (HCC) tissues and its interaction with ERK1/2 signaling pathway, so as to establish a theoretical basis for further studying the molecular mechanism of MIF promoting HCC. Methods: From February 2020 to August 2021, 52 cases of hepatocellular carcinoma (HCC) tissues based on hepatitis B cirrhosis (HBV-LC) and 52 cases of adjacent tissues in Tianjin Medical University Cancer Hospital and 940th Hospital of Joint Logistic Support Force of PLA were collected as the experimental group, including 39 males and 13 females, aged 35-65 years. And 20 cases of normal liver tissue were selected as the control group. Immunohistochemistry was used to detect the expression of MIF, ERK1/2 and p-ERK1/2 proteins in liver tissues of the two groups, and in situ hybridization was used to detect the expression of ERK1/2 nucleic acid in liver tissues of the two groups.HepG2 HCC cells and L-02 normal hepatocytes were co-cultured with different concentrations of rMIF, the expression and phosphorylation levels of ERK1/2 and JNK1 proteins in the two kinds of liver cells were detected by Western-blot, and the expression levels of ERK1/2 nucleic acids in the two kinds of liver cells were detected by RT-PCR. One-way ANOVA was used for measurement data and χ2 test was used for counting data. Results: The expressions of MIF, ERK1/2, p-ERK1/2 and ERK1/2 mRNA were significantly increased in HCC and para-cancer tissues (the expression of MIF in HCC group was 78.8%, and that in adjacent group was 75.0%; ERK1/2 80.8% in HCC group and ERK1/2 71.8% in paracancerous group. The expression of p-ERK1/2 75.0 % in HCC group and 46.2% in paracancerous group were respectively detected. ERK1/2 mRNA was expressed in HCC group 76.9%, ERK1/2 mRNA expression in paracancerous group 78.8%), and the differences were statistically significant compared with normal liver tissues (P<0.05), but there was no significant difference between HCC and para-cancer tissues (P>0.05). The expressions of ERK1/2, p-ERK1/2 and ERK1/2 mRNA in HepG2 HCC cells were significantly increased with the increase of rMIF concentration, and the increase was most obvious when rMIF concentration was 200 ng/ml, and the difference was statistically significant compared with L-02 normal hepatocytes (P<0.05). Conclusion: MIF, ERK1/2 and p-ERK1/2 are highly expressed in HCC tissues and HepG2 HCC cells, suggesting that MIF promotes the occurrence and development of hepatocellular carcinoma through ERK1/2 signaling pathway.


Asunto(s)
Carcinoma Hepatocelular , Neoplasias Hepáticas , Factores Inhibidores de la Migración de Macrófagos , Femenino , Humanos , Masculino , Carcinoma Hepatocelular/diagnóstico , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patología , Oxidorreductasas Intramoleculares/metabolismo , Neoplasias Hepáticas/diagnóstico , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patología , Factores Inhibidores de la Migración de Macrófagos/genética , Factores Inhibidores de la Migración de Macrófagos/metabolismo , Sistema de Señalización de MAP Quinasas , ARN Mensajero/metabolismo , Transducción de Señal , Adulto , Persona de Mediana Edad , Anciano
4.
Zhonghua Yi Xue Za Zhi ; 101(48): 3932-3937, 2021 Dec 28.
Artículo en Chino | MEDLINE | ID: mdl-34954994

RESUMEN

Objective: To investigate the risk factors associated with in-hospital mortality in hospitalized patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Methods: A total of 6 668 patients hospitalized for AECOPD in seven tertiary hospitals from September 2017 to January 2021 were consecutively included, and clinical data related to medical history, laboratory tests, treatment and prognosis were collected, and patients were divided into death group and survival group according to whether they died during hospitalization. After univariate analysis, multivariate logistic regression analysis was then performed to explore the independent risk factors related to in-hospital mortality. Results: Among 6 668 patients hospitalized for AECOPD, 128 patients experienced in-hospital death, with a mortality rate of 1.9%. The mean age of the death group was (81±9) years, which was significantly older than that of the survival group ((72±11) years P<0.001). The proportion of patients in the AECOPD in-hospital death group with a combination of prolonged bed rest, hypertension, myocardial infarction within 3 months, cardiac insufficiency, chronic pulmonary heart disease, pneumonia, type 2 diabetes, venous thromboembolism (VTE), and chronic renal insufficiency was also significantly higher than in the survival group (all P<0.05) The median length of stay in the in-hospital death group was 18 d, which was significantly longer than that in the survival group (9 d, P<0.001), and the proportion of patients admitted to the ICU, receiving invasive mechanical ventilation and non-invasive mechanical ventilation was also significantly higher than that in the survival group (all P<0.05). The white blood cell count, glutamic transaminase, blood creatinine, calcitoninogen, C-reactive protein, D-dimer, N-terminal B-type natriuretic and Pseudomonas aeruginosa infection rates were significantly higher than those in the survival group (all P<0.05). Multifactorial analysis showed that age>80 years (OR=3.82, 95%CI 2.36 to 6.18, P<0.001), prolonged bed rest (OR=2.95, 95%CI: 1.79 to 4.86, P<0.001), chronic pulmonary heart disease (OR=1.85, 95%CI: 1.14 to 3.00, P=0.012), and pneumonia (OR=2.75, 95%CI: 1.65 to 4.60, P<0.001), invasive mechanical ventilation (OR=7.33, 95%CI: 4.40 to 12.21, P<0.001), noninvasive mechanical ventilation (OR=3.73, 95%CI: 2.30 to 6.04, P<0.001), anemia (OR=2.03. 95%CI: 1.21 to 3.42, P=0.008), and calcitoninogen>0.5 ng/ml (OR=2.38, 95%CI: 1.41 to 4.02, P=0.001) were independent risk factors for in-hospital mortality in patients with AECOPD. Conclusion: Advanced age (>80 years), prolonged bed rest, chronic pulmonary heart disease, pneumonia, invasive mechanical ventilation, noninvasive mechanical ventilation, anemia, and calcitoninogen>0.5 ng/ml were independent risk factors for in-hospital mortality in patients hospitalized with AECOPD.


Asunto(s)
Diabetes Mellitus Tipo 2 , Enfermedad Pulmonar Obstructiva Crónica , Anciano , Anciano de 80 o más Años , Mortalidad Hospitalaria , Humanos , Estudios Retrospectivos , Factores de Riesgo
5.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(6): 507-509, 2021 Jun 09.
Artículo en Chino | MEDLINE | ID: mdl-34098664

RESUMEN

The common chronic disorders in the world, such as cardiovascular diseases, diabetes mellitus, cancers, etc., are all the multi-factorial diseases. They are caused by the interactions of various risk factors among which inflammation plays an important role in the pathogenesis of all these chronic disorders. Any factor which contributes or enhances systemic inflammatory burdens may serve as the risk factors of these diseases. During periodontitis, the bacteremia and subsequent systemic dispersal of periodontal pathogens and bacterial components may elicit production of pro-inflammatory immune mediators, and the pro-inflammatory immune mediators produced locally at the periodontal lesion may also enter the systemic circulation. Behaving as a potential source for increased chronic systemic inflammatory challenge, periodontitis contributes as the risk factor of these systemic inflammation associated chronic diseases.


Asunto(s)
Enfermedades Cardiovasculares , Diabetes Mellitus , Periodontitis , Humanos , Inflamación , Factores de Riesgo
6.
Zhonghua Gan Zang Bing Za Zhi ; 29(2): 137-142, 2021 Feb 20.
Artículo en Chino | MEDLINE | ID: mdl-33685082

RESUMEN

Objective: To investigate the safety and efficacy of voriconazole in the patients with cirrhosis at Child-Pugh C stage complicated by invasive fungal infection(IFI). Methods: A retrospective collection of medical records of 76 patients with cirrhosis at Child-Pugh C stage complicated by IFI who were admitted to our hospital, from August 2014 to August 2017 was carried out. All the 76 patients who used voriconazole to treat IFI were divided into recommended dose group for hepatic insufficiency(56 cases) and routine dose group(20cases). The two groups were observed and compared in terms of the voriconazole's plasma concentrations, the outcomes of IFI and the rate of untoward reactions. The liver functional indicators were also compared between before and after treatment each group. We used Student's t test, Z test, chi-square test, or Fisher's exact test, as appropriate, for statistical analysis. Results: Both groups had good performance and low frequencies of side effects in the treatment of IFI, but there were also significant differences in the plasma concentrations of voriconazole and the incidence of untoward reactions between the two groups(P = 0.008 and P = 0.022). There commended dose group for hepatic insufficiency had lower adverse effect rate. The levels of direct bilirubin, alanine aminotransferase and aspartate aminotransferase were significantly lower after treatment of IFI in the recommended dose group for hepatic insufficiency(P < 0.05). Conclusion: In our research, it is relatively safe and effective to use voriconazole to treat IFI in the patients with cirrhosis at Child-Pugh C stage if according to the recommended dose regimen for cirrhosis at Child-Pugh A,B stage.


Asunto(s)
Antifúngicos , Infecciones Fúngicas Invasoras , Antifúngicos/uso terapéutico , Humanos , Infecciones Fúngicas Invasoras/tratamiento farmacológico , Cirrosis Hepática/complicaciones , Cirrosis Hepática/tratamiento farmacológico , Estudios Retrospectivos , Voriconazol/uso terapéutico
7.
Zhonghua Yu Fang Yi Xue Za Zhi ; 55(3): 371-378, 2021 Mar 06.
Artículo en Chino | MEDLINE | ID: mdl-33730830

RESUMEN

Objective: To explore the flora characteristics and differences of esophageal tissues between elderly esophageal squamous cell carcinoma (ESCC) patients and young and middle-aged ESCC patients, so as to assist in studying the potential biomarkers of elderly ESCC patients. Methods: In this study, a retrospective study was adopted. 72 ESCC patients diagnosed in Taihe Hospital, Shiyan City, Hubei Province from July 2018 to July 2019 were selected, including 49 patients in the elderly group (≥ 60 years old, 40 males and 9 females), 23 patients in the young and middle-aged group (<60 years old, 21 males and 2 females). In the same period, 20 healthy persons without abnormal gastroscopy in endoscopy center were selected as the control group (aged 35-78 years old, median age 57 years old, 16 males and 4 females). The genomic DNA was extracted from the affected esophageal tissues of patients with ESCC and the middle esophageal samples of the control group. The V4 hypervariable region of bacterial 16SrRNA gene sequence was amplified. Illumina HiSeq sequencing technology was adopted. The flora characteristics of elderly, young and middle-aged ESCC patients was compared and analyzed. QIIME and Rstudio software were used to analyze the sequence data, and nonparametric Kruskal-Wallis test or Wilcoxon rank sum test were used for statistical methods. Results: Shannon index [5.17 (4.53, 5.95) vs. 4.79 (3.74, 5.97)], Simpson index [0.94 (0.91, 0.96) vs. 0.92 (0.83, 0.96)] and Chao1 index [343.55 (259.76, 570.59) vs. 329.16 (268.88, 648.00)] were similar in flora of two groups, and there was no significant difference (Z=-0.791, -1.057, -0.380, all P>0.05). There was no significant difference in ß-diversity between the elderly group and the young and middle-aged group (PC1=19.14%, PC2=6.95%, PPC1=0.67, PPC2=0.42). At the phyla level, the top 5 phyla in abundance were as follows: Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria and Fusobacteria in the young and middle-aged group, while the top 5 phyla in abundance were as follows: Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria and Actinobacteria in the elderly group; the significant difference between the two groups was Fusobacteria (Q=0.596, P<0.05). At the genus level, the top 5 genera in the young and middle-aged group in abundance were as follows: Prevotella, Bacteroides, Streptococcus, Selenomonas and Veillonella. In the elderly group, Prevotella, Bacteroides, Streptococcus, Selenomonas and Haemophilus were the top 5 in abundance, and there were significant difference in Fusobacterium between the two groups (Q=0.938, P<0.05). PICRUSt function prediction showed that the abundance of Aminoacyl.tRNA.biosynthesis, Nucleotide.excision.repair, RNA.polymerase, Ribosome, Clavulanic.acid.biosynthesis, Photosynthesis and Photosynthesis. proteins in the elderly group were lower than those in the young and middle-aged group (all Q=0.734, P<0.05). Conclusion: There is no significant difference in α-diversity and ß-diversity between elderly ESCC patients and young and middle-aged patients, but the abundance of Fusobacterium flora increased.


Asunto(s)
Neoplasias Esofágicas , Carcinoma de Células Escamosas de Esófago , Adulto , Anciano , Neoplasias Esofágicas/genética , Carcinoma de Células Escamosas de Esófago/genética , Femenino , Humanos , Masculino , Metagenómica , Persona de Mediana Edad , ARN Ribosómico 16S/genética , Estudios Retrospectivos
8.
Zhonghua Wei Chang Wai Ke Za Zhi ; 24(2): 173-178, 2021 Feb 25.
Artículo en Chino | MEDLINE | ID: mdl-33508924

RESUMEN

Objective: Anatomic variations in the perigastric vessels during laparoscopic radical gastrectomy often affect the operator's judgment and prolong the operation time, and even cause accidental injury and surgical complications, and hence the safety and quality of the operation cannot be ensured. In this study, multiple slice CT was reconstructed by 3-dimensional CT simulation software (3D-CT), and 3D-CT images were used to describe the variation of celiac trunk and splenic artery before surgery. The guiding role of the different variation of vessels was analyzed for laparoscopic total gastrectomy+D2 lymph node dissection (LTG+D2LD). Methods: A retrospective cohort study was conducted. Case inclusion criteria: (1) Gastric cancer was at an advanced stage. All the patients were preoperatively examined by digestive endoscopy and 64-row enhanced CT scan, and were histopathologically diagnosed with gastric adenocarcinoma. (2) 3D-CT simulation images were reconstructed to guide the operation. (3) LTG+D2LD surgery was performed by the same surgical team. (4) Clinical data were complete, and all the patients had signed the informed consent. From 2014 to 2018, 98 patients with gastric cancer at the Gastrointestinal Surgery Department of Henan Provincial People's Hospital were enrolled. According to the Adachi classification, celiac trunk variation was divided into common type (Adachi type I) and rare type (Adachi type II-VI). According to the Natsume classification, splenic artery was classified into "flat type" and "curved type". Based on 3D-CT simulation images, variation of celiac trunk and splenic artery was described, and the differences in operation time, intraoperative blood loss and the number of postoperative retrieved lymph nodes were compared between groups with different types of arterial variation. Results: For celiac trunk, common type was found in 84 cases (86%) and rare type was found in 14 cases, including 6 cases (6%) of type II, 2 cases (2%) of type III, 2 cases (2%) of type IV, 3 cases (3%) of type V, 1 case (1%) of type VI. No other types were found. There were no statistically significant differences in clinical characteristics and number of retrieved lymph nodes between patients of the common type group and rare type group (all P>0.05). Compared with common type patients, those of rare type had longer operative time [(321.1±29.0) minutes vs. (295.1±46.5) minutes, t=2.081, P=0.040] and more intraoperative blood loss (median: 66.0 ml vs. 32.0 ml, Z=-4.974, P=0.001). For splenic artery, 41 patients (42%) were flat type and 57 patients (58%) were curved type. There were no statistically significant differences between the two groups in terms of clinical characteristics, intraoperative blood loss, operative time and number of retrieved lymph nodes (all P>0.05). Conclusions: The method of describing the variation in the perigastric vessels by 3D-CT simulation has certain clinical value in laparoscopic radical gastrectomy. The duration of LTG+D2LD is prolonged and the intraoperative blood loss is increased with the variation of celiac trunk, while the variation of splenic artery has no effect on LTG+D2LD.


Asunto(s)
Gastrectomía , Artería Gástrica/diagnóstico por imagen , Laparoscopía , Neoplasias Gástricas , Estómago/diagnóstico por imagen , Simulación por Computador , Artería Gástrica/anatomía & histología , Humanos , Imagenología Tridimensional , Escisión del Ganglio Linfático , Estudios Retrospectivos , Estómago/irrigación sanguínea , Neoplasias Gástricas/irrigación sanguínea , Neoplasias Gástricas/diagnóstico por imagen , Neoplasias Gástricas/cirugía , Tomografía Computarizada por Rayos X
9.
Zhonghua Zhong Liu Za Zhi ; 43(1): 118-125, 2021 Jan 23.
Artículo en Chino | MEDLINE | ID: mdl-33472324

RESUMEN

Objective: To investigate the effect of pentraxin 3 (PTX3) on the proliferation, invasion and drug resistance of pediatric neuroblastoma cells and its mechanism. Methods: si-RNA (si-RNA group), si-PTX3 (si-PTX3 group), siRNA+ pcDNA3.1 (siRNA+ pcDNA3.1 group), si-PTX3+ pcDNA3.1 (si-PTX3+ pcDNA3.1 group), siRNA+ pcDNA3.1-Toll-like receptor 4 (siRNA+ pcDNA3.1-TLR4 group) and si-PTX3+ pcDNA3.1-TLR4 (si-PTX3+ pcDNA3.1-TLR4 group) were transfected into SH-SY5Y cells. Collected 32 cases of tumor tissue and cancerous tissue in children with childhood neuromaternal cells who were treated at Zhumadian center hospital from July 2016 to August 2019. Real-time fluorescent quantitative polymerase chain (RT-qPCR) reaction and immunohistochemistry experiments were used to detect the protein expressions of PTX3 in neuroblastoma tissues and normal tissues. 5-Ethynyl-2'-deoxyuridine (EdU) was used to detect the proliferation effect of PTX3 on neuroblastoma cell SH-SY5Y. Western blot experiment was used to detect the protein expression levels of vascular endothelial growth factor (VEGF), resistance-related proteins including P-glycoprotein (P-gp) and multidrug resistance-associated protein 1 (MRP-1), and invasion-related protein matrix metalloproteinase-1 (MMP-1). Results: PTX3 mRNA expressions in neuroblastoma tissues were 0.87±0.07, higher than 0.13±0.06 of normal tissues, and the differences were statistically significant (P<0.05), The expression of the immunohistochemistry test PTX3 protein was consistent with the qRT-PCR results. Compared with the si-RNA group (0.95±0.08; 1.02±0.10), the mRNA and protein expressions of PTX3 in the si-PTX3 group (0.25±0.05; 0.45±0.66) decreased, the differences were statistically significant (all P<0.05). The number of EdU positive cells, invasion rate, VEGF, MMP-1, P-gp and MRP-1 protein expressions in si-RNA group were (31.86±1.86)%, (28.12±2.96)%, (0.58±0.07), (0.44±0.06), (0.46±0.08) and (0.51±0.05), respectively, higher than (19.73±1.22)%, (8.45±1.06)%, (0.25±0.05), (0.19±0.03), (0.19±0.06) and (0.16±0.07) in si-PTX3 group, and the differences were statistically significant (all P<0.05). The Number of EdU positive cells [(19.49±1.68)%], invasion rate [(8.48±1.36)%], VEGF protein expression (0.10±0.15), P-gp (0.18±0.07) , TLR4 (0.45±0.06), p-p65 (0.25±0.05) protein expressions in si-PTX3+ pcDNA3.1 group were relatively lower compared with siRNA+ pcDNA3.1 group [(38.21±2.67)%, (26.39±2.14)%, 0.49±0.05, 0.52±0.06, 0.93±0.14 and 0.82±0.06] (all P<0.05). The number of EdU-positive cells [(62.73±5.18)%], invasion rate [(50.45±3.25)%], VEGF protein expression (2.17±0.17), P-gp (2.15±0.16), TLR4 (2.68±0.16), p-p65 (2.48±0.13) protein expressions in the siRNA+ pcDNA3.1-TLR4 group increased compared with siRNA+ pcDNA3.1 group (all P<0.05). Conclusions: Inhibition of PTX3 can inhibit the proliferation and invasion of neuroblastoma cells SH-SY5Y, and reduce drug resistance. Its mechanism may be achieved by regulating the TLR4/NF-κB signaling pathway. This result can provide a new perspective for pediatric neuroblasts tumor diagnosis and clinical treatment.


Asunto(s)
FN-kappa B , Neuroblastoma , Proteína C-Reactiva , Proliferación Celular , Niño , Resistencia a Medicamentos , Humanos , FN-kappa B/metabolismo , Neuroblastoma/genética , Componente Amiloide P Sérico , Transducción de Señal , Receptor Toll-Like 4/genética , Factor A de Crecimiento Endotelial Vascular/genética
11.
Phys Chem Chem Phys ; 23(2): 1335-1342, 2021 Jan 21.
Artículo en Inglés | MEDLINE | ID: mdl-33367358

RESUMEN

Although minor alloying in metallic glasses (MGs) has been extensively investigated, the effect of O doping is still a debatable topic. In the present study, the atomic-level structures and mechanical properties of Zr-based MGs doped with different O contents have been analyzed using ab initio molecular dynamics simulations. It is revealed that O atoms prefer to bond to Zr atoms due to their low mixing enthalpy, and that O atoms degrade the properties of Zr-lean MGs but hardly affect the properties of Zr-rich MGs, with results suggesting a compositional dependence of O doping. For Zr-lean MGs, the fraction of full icosahedra, size of the medium-range-order clusters, Young's modulus and shear modulus decrease sharply with O content, while accompanied by a sharp increase of the non-Frank-Kasper polyhedra, and the ratio of bulk modulus to shear modulus and Poisson's ratio, indicating decreased strength and improved plasticity. For Zr-rich MGs, however, the above-mentioned structural and mechanical features experience little change or only change slightly after O doping, showing low oxygen sensitivity. It is shown that the high Zr content weakens the effect of Zr-O bonding to some extent. The present study not only sheds light on the atomic-level structures of O-doped MGs, which may provide guidelines for designing MGs with low-grade materials, but also helps to explain the previous conflicting results based on the composition-dependence effect.

12.
Occup Med (Lond) ; 70(8): 570-577, 2020 Dec 12.
Artículo en Inglés | MEDLINE | ID: mdl-33108459

RESUMEN

BACKGROUND: Although the association of cannabis use with automobile accidents has been well-studied, the impact of cannabis on workplace safety and injuries is less clear. AIMS: The purpose of this study was to examine the relationship between work-related injury and cannabis use in the past year. METHODS: We performed a cross-sectional analysis of the Canadian Community Health Survey (2013-16) of working individuals. We used multiple logistic regression modelling to calculate the odds of experiencing a work-related injury (defined as non-repetitive strain injury) among workers who reported using cannabis more than once during the prior 12 months as compared to non-users. We repeated the analysis among participants working in high injury risk occupational groups only. RESULTS: Among the 136 536 working participants, 2577 (2%) had a work-related injury in the last 12 months. Of these 2577 who had a work-related injury, 4% also reported being a cannabis user in the same period. We found no association between past-year cannabis use and work-related injury (odds ratio for work injury among users 0.81, 95% confidence interval 0.66-0.99). The association was unchanged in the subgroup analysis limited to high injury risk occupational groups. CONCLUSIONS: We found no evidence that cannabis users experienced higher rates of work-related injuries. While awaiting prospective studies, occupational medicine practitioners should take a risk-based approach to drafting workplace cannabis policies.


Asunto(s)
Cannabis , Traumatismos Ocupacionales , Canadá , Estudios Transversales , Humanos , Masculino , Estudios Prospectivos
14.
Transl Psychiatry ; 10(1): 295, 2020 08 21.
Artículo en Inglés | MEDLINE | ID: mdl-32826855

RESUMEN

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

15.
Zhonghua Wei Chang Wai Ke Za Zhi ; 23(7): 653-656, 2020 Jul 25.
Artículo en Chino | MEDLINE | ID: mdl-32683825

RESUMEN

D2 lymphadenectomy combined with complete mesentery excision (CME) for advanced gastric cancer in recent years was a hotspot issue in China, while its safety and effectiveness have been proved. According to the Membrane anatomy of the stomach, both surgical approach and mesogastrium interval is particularly important in Laparoscopic radical gastrectomy. We summarized and shared the following clinical experience for medical colleagues. (1) Lymph nodes of right abdominal aorta-No.7,8,9,12-should be resection as an indivisible whole. This integrity tissue above the portal vein was supposed to the end of the dorsal mesentery of stomach and the continuation of Gerota fascia. (2) No.10 (splenic hilar lymph nodes) lymphadenectomy: The surgical approach enters the Gerota fascia between the left gastric artery(LGA) and the left alongside the splenic artery. When the extent of lymphadenectomy performed to cardia and upper margin of the spleen, then the ultrasonic scalpel should excise the lymph node along the splenic artery to the splenic hilum. (3) Esophagogastric junctional cancer: There is no consensus over the type of resection and the extent of lymphadenectomy that could be a standard of care for this category.While we recommended that paraesophageal lymph node dissection and digestive tract reconstruction should be completed in 3D laparoscopy vision. (4) Infracardiac bursa(ICB): Intentional entry into the ICB provides surgeons with a landmark to identify the location of the pleura, and inferior vena cava. (5)The application of endoscopic aspirator with flushing and electrocautery. The CME concept of gastric cancer emphasizes the membrane anatomy theory rather than the regional lymph node. The precision and homogeneity of the D2 procedure therapy of gastric cancer depend on complete mesentery excision, standard the surgical process, or approach. Only in this way can we find the avascular gaps easily and perfectly cover the extent of lymph node dissection required for the D2 procedure.


Asunto(s)
Gastrectomía/normas , Escisión del Ganglio Linfático/normas , Mesenterio/cirugía , Neoplasias Gástricas/cirugía , China , Competencia Clínica , Fascia , Gastrectomía/métodos , Artería Gástrica/cirugía , Humanos , Laparoscopía , Escisión del Ganglio Linfático/métodos , Mesenterio/anatomía & histología , Mesenterio/irrigación sanguínea , Mesenterio/patología , Vena Porta/cirugía , Arteria Esplénica/cirugía , Neoplasias Gástricas/patología
16.
Eur Rev Med Pharmacol Sci ; 24(3): 1504-1514, 2020 02.
Artículo en Inglés | MEDLINE | ID: mdl-32096200

RESUMEN

OBJECTIVE: In this study, we investigated whether certain types of lipid profiles are major contributors of acute ischemic stroke (AIS). PATIENTS AND METHODS: We screened 13,285 hospitalized patients in two stroke medical centers treated with thrombolysis, thrombectomy, or conventional care for anterior cerebral artery-occluded AIS, and found 266 patients. We examined their plasma lipid profiles using the cutoff values from a receiver operating characteristic (ROC) curve. We applied a multivariate logistic regression or Fisher's exact test to compare their outcomes and risk factors. We used the modified Rankin scale (mRS) score to assess the major clinical outcome of the patients 3 months after disease onset. Mortality and symptomatic intracranial hemorrhage (sICH) were both evaluated as risk factors. We analyzed symptoms' improvements at discharge as a disease outcome measure. RESULTS: In the patients with anterior cerebral artery-occluded AIS (NIHSS ≥ 10) treated by intravenous (IV) thrombolysis, a total cholesterol (TC) level greater than 5.07 mmol/L predicted a poor outcome (OR 3.55, 95% CI 1.21,10.46, p=0.021). CONCLUSIONS: In patients with anterior cerebral artery-occluded AIS, the TC level is a promising prognosis marker for the IV thrombolysis outcome.


Asunto(s)
Isquemia Encefálica/sangre , Trastornos Cerebrovasculares/sangre , Colesterol/sangre , Fibrinolíticos/administración & dosificación , Accidente Cerebrovascular Isquémico/sangre , Terapia Trombolítica/tendencias , Anciano , Arteria Cerebral Anterior/diagnóstico por imagen , Isquemia Encefálica/diagnóstico por imagen , Isquemia Encefálica/tratamiento farmacológico , Trastornos Cerebrovasculares/diagnóstico por imagen , Trastornos Cerebrovasculares/tratamiento farmacológico , Femenino , Humanos , Accidente Cerebrovascular Isquémico/diagnóstico por imagen , Accidente Cerebrovascular Isquémico/tratamiento farmacológico , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Terapia Trombolítica/métodos , Activador de Tejido Plasminógeno/administración & dosificación , Resultado del Tratamiento
17.
Eur Rev Med Pharmacol Sci ; 23(19): 8573-8579, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31646590

RESUMEN

OBJECTIVE: The aim of this study was to investigate the effect of long non-coding ribonucleic acid (lncRNA) growth arrest specific 5 (GAS5) on acute myocardial infarction (AMI) model rats and to explore its regulatory mechanism. MATERIALS AND METHODS: The rat model of AMI was established by subcutaneous injection of isoproterenol (ISO). 30 Sprague Dawley (SD) rats were randomly divided into three groups, including Control group, Model group, and lncRNA GAS5 inhibitor [small interfering ribonucleic acid (siRNA) GAS5] group. Hematoxylin and eosin (H&E) staining was used to detect the pathological damage of myocardial tissues in rats of each group. Myocardial cell apoptosis in each group determined via terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The levels of matrix metalloproteinase (MMP)-2 and MMP-9 in the serum of rats in each group were examined by enzyme-linked immunosorbent assay (ELISA). Reverse transcription-polymerase chain reaction (RT-PCR) was adopted to measure the expression level of miR-21 in rat myocardial tissues. RESULTS: Compared with Control group, rats in Model group had significantly poor cardiac function, serious pathological damage of myocardial tissues, as well as increased apoptosis rate of myocardial cells. Meanwhile, the levels of MMP-2 and MMP-9 were significantly elevated in serum of Model group, while miR-21 level was down-regulated. In comparison with Model group, rats in siRNA GAS5 group exhibited significantly improved cardiac function, alleviated pathological damage to myocardial tissues, as well as decreased apoptosis rate of myocardial cells. Furthermore, the levels of MMP-2 and MMP-9 decreased significantly in serum of siRNA GAS5 group, whereas the expression level of miR-21 in myocardial tissues was down-regulated. CONCLUSIONS: SiRNA GAS5 can enhance the cardiac function of AMI model rats, relieve pathological damage, reduce myocardial cell apoptosis, and inhibit the occurrence of myocardial fibrosis. The possible underlying mechanism may be associated with up-regulation of miR-21.


Asunto(s)
MicroARNs/metabolismo , Infarto del Miocardio/metabolismo , ARN Largo no Codificante/metabolismo , Enfermedad Aguda , Animales , Modelos Animales de Enfermedad , Inyecciones Subcutáneas , Isoproterenol/administración & dosificación , MicroARNs/genética , Infarto del Miocardio/inducido químicamente , ARN Largo no Codificante/genética , Ratas , Ratas Sprague-Dawley
18.
Eur Rev Med Pharmacol Sci ; 23(3 Suppl): 171-176, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-31389588

RESUMEN

OBJECTIVE:   To investigate the intervention of exogenous adiponectin in the elderly rats with cognitive dysfunction induced by isoflurane through mitogen-activated protein kinase (MAPK) signaling pathway in hippocampus. MATERIALS AND METHODS: A total of 60 healthy elder Sprague Dawley (SD) rats aged 15-20 months and weighing 400-500 g were selected. These rats were randomly divided into four groups, i.e., the control group, the anesthetic group, adiponectin intervention group, and p38-MAPK antagonist group, in which the rats in the control group were treated through inhalation of pure oxygen for 4 h at a rate of 4 L/min, while the rats in the other 3 groups were treated through inhalation of isoflurane for 4 h. During the inhalation of isoflurane, the concentration of isoflurane was 3.5% at the beginning and decreased to 2.2% at 1 h, and 1.7% between 2 h and 4 h. Then, the intraperitoneal injection of 0.5 mL normal saline was performed for the rats in the control group and the anesthetic group, while adiponectin (300 mg/kg) was injected into the rats in the adiponectin intervention group and p38-MAPK antagonist group. Simultaneously, the antagonist (20 mg/kg) diluted to 0.5 mL was given to the rats in the p38-MAPK antagonist group, once/day for 3 days. Morris water maze test was carried out respectively in the 1st, 3rd, and 7th day, and 5 rats participated in the test at each time point, during which we recorded the escape latency, as well as the length of the swimming route of rats. Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting were employed to detect the mRNA and protein expressions of p38 in the hippocampus. RESULTS: The escape latency and the length of the swimming route at any time point after the intervention in the anesthetic group were significantly longer than those in the control group (p<0.05), and they were significantly shorter in the adiponectin intervention group than those in the anesthetic group (p<0.05), but there were no differences between the p38-MAPK antagonist group and the anesthetic group (p>0.05). The mRNA and protein expressions of p38 at any time point after intervention in the anesthetic group were higher than those in the control group (p<0.05), and they were significantly lower in the adiponectin intervention group than those in the anesthetic group (p<0.05), but there were no differences between the p38-MAPK antagonist group and the anesthetic group (p>0.05). CONCLUSIONS: Exogenous adiponectin can improve the cognitive dysfunction of the elderly rats after anesthesia using isoflurane, possibly by inhibiting the p38-MAPK signal pathway in hippocampus.


Asunto(s)
Adiponectina/metabolismo , Envejecimiento/metabolismo , Disfunción Cognitiva/tratamiento farmacológico , Hipocampo/metabolismo , Isoflurano/efectos adversos , Animales , Disfunción Cognitiva/inducido químicamente , Disfunción Cognitiva/metabolismo , Modelos Animales de Enfermedad , Hipocampo/efectos de los fármacos , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Masculino , Aprendizaje por Laberinto/efectos de los fármacos , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley , Resultado del Tratamiento
19.
Eur Rev Med Pharmacol Sci ; 23(13): 5705-5713, 2019 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-31298323

RESUMEN

OBJECTIVE: The aim of this study was to investigate whether long non-coding RNA (lncRNA) GClnc1 was involved in the development of colorectal cancer, and to explore its possible mechanisms. PATIENTS AND METHODS: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was conducted to detect GClnc1 expression in 48 colorectal cancer tissues and normal colon tissues. The Kaplan-Meier method was used to analyze the relationship between GClnc1 expression and survival rate of patients with colorectal cancer. In addition, GClnc1 expression in colorectal cancer cell lines and normal colonic epithelial cell lines were analyzed. After knockdown and over-expression of GClnc1 in colorectal cancer cells, Cell Counting Kit-8 (CCK-8) and colony formation assay were performed to detect the viability and proliferation of cells, respectively. RNA pull-down and RNA-binding protein immunoprecipitation (RIP) were applied to examine the specific interaction between GClnc1 and p53. After over-expression of GClnc1 in colorectal cancer cells, qPCR and Western blot were performed to evaluate the expression levels of p53, p21 and BAX. Meanwhile, the Luciferase reporter gene assay was established to reveal the activity of p53 after over-expression of GClnc1. ChIP assay was applied to figure out whether GClnc1 could affect the binding ability of p53 to the promoter region of p21. After p53 or GClnc1 knock-down in colorectal cancer cells, the protein level of p53 was analyzed using Western blot. Finally, qRT-PCR, CCK-8 and colony formation assay were used to detect the levels of p21 and BAX, the viability, as well as the proliferation ability of cells, respectively. RESULTS: The expression of GClnc1 in colorectal cancer tissues was significantly higher than that of para-cancerous tissues. Meanwhile, GClnc1 expression in T3 and T4 tumors was markedly higher than that of T1 and T2. The survival analysis revealed that patients with a higher level of GClnc1 showed remarkably lower overall survival than those with lower expression of GClnc1. QRT-PCR results indicated that GClnc1 expression in colorectal cancer cells (including SW620 and HCT116) was conspicuously higher than that of normal colonic epithelial cells (NCM640). After knocking down GClnc1 in SW620 cells, the viability and proliferation abilities were conspicuously decreased. Meanwhile, the expression level of GClnc1, as well as the viability and colony formation ability of cells, were significantly increased after over-expression of GClnc1 in HCT116 cells. Subsequently, the qRT-PCR assay demonstrated that GClnc1 was mainly localized in the nucleus. RNA pull-down and RIP experiments revealed that there was a specific interaction between GClnc1 and p53. Moreover, qRT-PCR and Western blot analysis indicated that the expression level of p53 was not affected after over-expression of GClnc. However, the expressions of p21 and BAX were remarkably decreased. The Luciferase reporter gene assay revealed that GClnc1 over-expression markedly weakened the Luciferase activity of p53. Meanwhile, ChIP experiments demonstrated that GClnc1 up-regulation affected the binding condition of p53 to p21. Western blot analysis showed that knockdown of p53 reversed the increased mRNA level of p21 as well as BAX. Furthermore, p53 down-regulation significantly weakened cell viability and colony formation ability caused by knockdown of GClnc1. CONCLUSIONS: LncRNA GClnc1 was highly expressed in colorectal cancer tissues. Meanwhile, it could increase the proliferation of colorectal cancer cells by reducing the expression of p21 as well as BAX via p53 signaling pathway, thereby promoting the progression of colorectal cancer.


Asunto(s)
Neoplasias Colorrectales/metabolismo , ARN Largo no Codificante/metabolismo , Superóxido Dismutasa/metabolismo , Proteína p53 Supresora de Tumor/antagonistas & inhibidores , Sitios de Unión , Proliferación Celular , Células Cultivadas , Neoplasias Colorrectales/diagnóstico , Células HCT116 , Humanos , ARN Largo no Codificante/genética , Transducción de Señal/genética , Superóxido Dismutasa/genética , Proteína p53 Supresora de Tumor/genética , Proteína p53 Supresora de Tumor/metabolismo
20.
Med Oral Patol Oral Cir Bucal ; 24(2): e236-e242, 2019 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-30818317

RESUMEN

BACKGROUND: The purpose of the study was to compare the differences of the subjective satisfaction of the donor site morbidity between the free radial forearm flap (FRFF) and anterolateral thigh flap (ALTF) for tongue reconstruction. MATERIAL AND METHODS: One hundred and nineteen patients underwent FRFF or ALTF reconstruction were retrospectively evaluated by a standardized self-established donor site morbidity questionnaire which included 5 domains, sensibility, movement disabilities, cosmetics, social activities and general impacts on the quality of life. RESULTS: The Cronbach's coefficient alpha of the questionnaire was 0.707. The exploratory factor analysis revealed that the 5 items of the questionnaire might load onto two distinct subscales. Patients with ALTF had higher scores in the sensibility, cosmetics and the composite score (P < 0.05). No significant differences were found in the movement disabilities, social activities and general impacts on the quality of life between the two groups (P > 0.05). CONCLUSIONS: ALTF has the advantage of better results of donor site morbidity, such as sensibility and cosmetics, over FRFF.


Asunto(s)
Antebrazo/cirugía , Satisfacción del Paciente , Procedimientos Quirúrgicos Reconstructivos/métodos , Colgajos Quirúrgicos , Muslo/cirugía , Neoplasias de la Lengua/cirugía , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Morbilidad , Complicaciones Posoperatorias , Calidad de Vida , Reproducibilidad de los Resultados , Estudios Retrospectivos , Encuestas y Cuestionarios , Lengua/cirugía , Adulto Joven
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