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1.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 55(10): 716-721, 2020 Oct 09.
Artículo en Chino | MEDLINE | ID: mdl-33045781

RESUMEN

Three-dimensional (3D) printing prostheses or prosthodontic devices have been preliminarily used in the clinics of prosthodontics. These prostheses or devices include diagnostic prostheses, crowns and fixed bridges, removable denture frameworks, implant surgical template, and custom-made implants, etc. There are three major types of technologies for 3D printing according to the forming principles, namely, light curing, sintering or melting, and melting-condensation. The materials used in 3D printing prostheses or devices include polymers, metals, and ceramics, etc. The advantages of 3D printing prostheses include material-saving, customized manufacturing for restorations, and batch production. However, the precision and strength of prostheses or devices, long-term clinical effects, and grade of maturity in customized manufacturing need further investigation and improving. This article focused on the present application, and the future development of 3D printing technologies in prosthodontic treatments. It will provide beneficial references for the future studies, development, and clinical application of 3D printing technologies and restorations in prosthodontic fields.


Asunto(s)
Implantes Dentales , Prostodoncia , Cerámica , Coronas , Impresión Tridimensional
2.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 55(10): 729-736, 2020 Oct 09.
Artículo en Chino | MEDLINE | ID: mdl-33045783

RESUMEN

Objective: To explore and evaluate a three-dimensional (3D) digital simulated design and implementation technique in esthetic rehabilitation. Methods: Thirty patients with esthetic deficiency, who came to the Department of Prosthodontics, Peking University School and Hospital of Stomatology from December 2017 to July 2019, were recruited and randomly assigned into 2 groups. Four males and 11 females which were (36.0±10.5) years old in the experimental group, 6 males and 9 females which were (32.0±6.7) years old in the control group, were enrolled. In the experimental group, 3D digital simulated design was used to predict the post-treatment effect; and the final restorations were designed duplicating from the pre-treatment digital design confirmed by the patient and milled. In the control group, the final restorations were manufactured by the dental technician according to the design of two-dimensional (2D) digital smile design. The simulation degree of digital design and the similarity between preoperative design and postoperative rehabilitation were scored by the patients with visual analogue scales (VAS) in both groups; and the satisfaction rate to the restorations was evaluated by the patients. The quality of the restorations was accessed by a prosthodontist who did not know the grouping of patients according to the modified criteria of United States Public Health Service (USPHS). Results: Three-dimensional digital simulated design and implementation technique was successfully established. The VAS score on the simulation degree of digital design in the experimental group (8.5±0.5) was higher than that in the control group (7.2±0.7) (P<0.01); the VAS score on the similarity between preoperative design and postoperative rehabilitation in the experimental group (9.6±0.3) was higher than that in the control group (7.0±0.9) (P<0.01). The satisfaction rate of the patients to the restorations was significantly higher in the experimental group than in the control group (P<0.05). There was no significant difference of the quality of the restorations between the two groups on the anatomic form, the marginal adaption and the surface quality (P>0.05). Conclusions: Three-dimensional digital simulated design and implementation technique can help achieving 3D digital simulated design before treatment and duplication to the final restorations, and can improve the patients' satisfaction in esthetic rehabilitation.


Asunto(s)
Estética Dental , Sonrisa , Adulto , Odontólogos , Femenino , Humanos , Masculino , Persona de Mediana Edad , Satisfacción del Paciente , Prostodoncia , Estados Unidos
3.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 55(10): 743-749, 2020 Oct 09.
Artículo en Chino | MEDLINE | ID: mdl-33045785

RESUMEN

Objective: To quantitatively evaluate the change of the long axis angle and the relative displacement of the crown feature points of the posterior teeth under normal bite force utilizing an intraoral scanner, and to provide clinical reference. Methods: From May to December 2019, fifteen graduate volunteers (5 males and 10 females, aged from 22 to 30, with an average age of 25.7 years) from Peking University School and Hospital of Stomatology were recruited to participate in the study. The surface data (U1, L1) of the maxillary and mandibular posterior teeth were scanned by an intraoral scanner i500, and saved as original data. The volunteers were guided to bite in the intercuspal position with normal bite force. The buccal bite data of the posterior teeth were scanned as the basis for registration. The digital casts were imported into Geomagic studio 2013 software and the boundary lines along the gingival margin and mesial and distal contact area of posterior teeth of data (U1, L1) were determined. Long axis of the crown, crown centroid and mesial functional cusp vertex were establishd. The data (U1, L1) were segmented into single tooth. Single tooth was aligned to buccal bite data separately using best-fit alignment command based on the buccal common area of the crown and new casts data (U2, L2) were obtained as the data under bite force. The long axis angle and centroid distance between adjacent teeth (second premolar and first molar, second molar and first molar) were measured and the deviation between data obtained at mouth-open state and that at biting state was calculated. Negative value meant centroid distance became shorter under bite force. The first molar was set as the common area and registrate the U1, L1 to U2, L2. The angle of long axes, and displacement of centroid and of functional cusp vertex between second premolar of two casts were calculated, as well as between second molar of two casts. Wilcoxon signed rank test was used to analyze the differences of teeth displacement between second premolar and second molar, and between maxillary and mandibular jaws with SPSS 26.0 statistical software. Results: The result of second premolar and second molar in the same jaw had no statistical difference (P>0.05). The centroid distance deviation of mandibular second premolar-first molar [-0.022(0.046) mm] was larger than that of maxilla [-0.006 (0.040) mm] (P<0.05). The long axis angle of second premolar itself [0.913°(0.647°)] and centroid distance of second molar itself [0.102 (0.106) mm] on the mandibular jaw were different from that on the maxillary jaw, which were 0.590°(0.550°) and 0.074(0.060) mm respectively (P<0.05). Conclusions: Under bite force, displacement of the second premolar and second molar was present, including the displacement of centroids and deflection of long axes. The mandibular posterior teeth have larger displacement than the maxillary teeth.


Asunto(s)
Fuerza de la Mordida , Diente Molar , Adulto , Diente Premolar , Oclusión Dental , Femenino , Humanos , Masculino , Maxilar , Diente Molar/diagnóstico por imagen
4.
Beijing Da Xue Xue Bao Yi Xue Ban ; 52(4): 762-770, 2020 Aug 18.
Artículo en Chino | MEDLINE | ID: mdl-32773816

RESUMEN

OBJECTIVE: To compare the clinical efficacy of a kind of complete dentures fabricated using computer aided design/computer aided manufacturing (CAD/CAM) workflow with that of the complete denture fabricated using conventional workflow. METHODS: Twenty edentulous patients were included in this prospective, single-blind, self-controlled clinical trial. Two pairs of complete dentures were fabricated for each participant: one using the functional suitable denture (FSD) system with CAD/CAM, and the other using conventional fabrication workflow. In the final delivery appointment, the conventional dentures worn by the participant for 3 months at first. Then the participant swapped to wear digital denture for another 3 months. The patients' satisfaction and oral health related quality of life (OHRQoL) were measured using the visual analogue scale (VAS) and oral health impact profile (OHIP-20E) at baseline, 2 weeks, 1 month, 2 months, and 3 months following denture delivery. In addition, the masticatory efficiency of each pair of dentures was measured after three months adaptation period for each type of dentures. RESULTS: The VAS scores of the twenty patients in FSD denture group on general satisfaction, ease of cleaning, ability to speak, esthetics, stability and oral health status on these six domains were higher than that of conventional denture group 3 months after delivery. While the VAS cores in FSD denture group on comfort, ability to chew and ability to chew up were the same as those of conventional denture group. But there was no significant difference (P>0.05). The VAS score on each domain in FSD denture group was higher than that of conventional denture group 3 months after delivery, three domains of which reached statistical significance (P < 0.05), involving functional limitation, psychological discomfort and total. The masticatory performance of the FSD denture (1.20±0.54) was slightly higher than that for the conventional denture (1.16±0.53), but the difference did not reach statistical significance (P=0.691). CONCLUSIONS: The clinical efficacy of the FSD complete denture is comparable to that of the conventional complete denture. As for patient satisfaction and oral health related quality of life, FSD dentures received comparable scores as conventional complete dentures did.


Asunto(s)
Dentadura Completa , Calidad de Vida , Humanos , Satisfacción del Paciente , Estudios Prospectivos , Método Simple Ciego , Resultado del Tratamiento
5.
Beijing Da Xue Xue Bao Yi Xue Ban ; 52(2): 390-394, 2020 Apr 18.
Artículo en Chino | MEDLINE | ID: mdl-32306028

RESUMEN

Fabrication of conventional complete dentures involves a complex restoration method, requiring significant time and typically involving primary impressions, definitive impressions, jaw relation records, clinic try-in, and complete denture placement, which has been used for nearly a century without change. A novel digital system named Functionally Suitable Denture (FSD) was researched and developed so as to reduce clinical steps, operation difficulties and errors of complete denture restoration. It pioneered a unique diagnostic complete denture aided by computer aided design (CAD) & 3D printing, by which, the functional impression, jaw relation, and try-in (3 steps) were simplified to 1 step, thus the number of visits to the dentist was reduced by 2 times. Moreover, for the first time, it put forward a CAD software of template matching based on the expert design, which was an efficient and intelligent design scheme, and the excellent denture experts' experience and skills could be inherited and iterated. The system included the 3D scanner with appropriate accuracy and high efficiency, the CAD software, the special 3D printer and process software, and the innovative clinical operation process. The Patent Cooperation Treaty (PCT) patent international search report showed that all the 15 claims of the technology were of novelty, creativity and industrial utility. All the digital products were independently developed and made by Peking University School and Hospital of Stomatology, China. The design and manufacture process of denture prosthesis was fast, simple and accurate. At the same time, personalized functional and aesthetic matching of the patients after wearing prosthesis was realized. It effectively solved the global problems of "slow, difficult and inaccurate" of the traditional manual technology of complete denture, and brought good news to edentulous patients. Compared with the traditional complete denture treatment, FSD system has a wide range of applications for different types of edentulous patients, including those with severe resorption of the alveolar ridge or a high occlusal force. Furthermore, the low-cost of 3D printers, compared with expensive milling machines, may make the approach more accessible. This review describes that our research is related to the development of the FSD system, including multi-source data acquisition technology, three generations of complete denture design software, 3D printing systems of individual tray and complete denture pattern, the clinical and laboratory operation process of the FSD system.


Asunto(s)
Boca Edéntula , China , Diseño Asistido por Computadora , Creatividad , Dentadura Completa , Humanos
6.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 55(3): 206-211, 2020 Mar 09.
Artículo en Chino | MEDLINE | ID: mdl-32193921

RESUMEN

Stem cell-derived exosomes and microvesicles are nanoscale extracellular vesicles which have merged as mediators of intercellular communication through delivering bioactive substances. They have showed promising potential in regenerative therapy due to their capacity in promoting tissue regeneration. It has been reported that stem cell-derived extracellular vesicles could enhance bone regeneration. The underlying mechanisms included inhibiting osteocyte apoptosis induced by ischemia and hypoxia, enhancing the proliferation, migration, and osteogenic differentiation of stem cells, and promoting angiogenesis. Further investigations indicated that microRNA seemed to play an important role in exosome-mediated regeneration. In this review, we summarized the biogenesis, components and functions of stem cell-derived extracellular vesicles as well as the current progress of their mechanisms and applications in bone regeneration.


Asunto(s)
Regeneración Ósea , Exosomas , Vesículas Extracelulares , Células Madre/citología , Humanos , Neovascularización Fisiológica , Osteogénesis
7.
Beijing Da Xue Xue Bao Yi Xue Ban ; 52(1): 1-9, 2020 Feb 18.
Artículo en Chino | MEDLINE | ID: mdl-32071456

RESUMEN

OBJECTIVE: To identify the role of Tribbles pseudokinase 3 (TRIB3) during the process of adipogenic differentiation of human adipose-derived mesenchymal stem cells (hASCs), and to provide a new target and a novel idea for the application of hASCs in adipose tissue engineering and soft tissue regeneration. METHODS: TRIB3-knockdown hASCs (shTRIB3) and TRIB3-overexpression hASCs (TRIB3-over) were established using lentivirus transfection technique. The transfection effect was estimated by the visible presence of green fluorescence as the expression of green fluorescent protein (GFP) in the transfected hASCs. The lentiviral transfection efficiency was examined by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. After adipogenic induction, Oil Red staining and quantification, as well as qRT-PCR about several specific adipogenic markers were used to evaluate the adipogenic differentiation ability of hASCs. RESULTS: In TRIB3-knockdown hASCs, the TRIB3 mRNA expression level decreased by about 84.3% compared with the control group (P<0.01), and the TRIB3 protein level also showed obvious reduction. Oppositely, in TRIB3-overexpression hASCs, the TRIB3 mRNA expression level increased by approximately 160% compared with the control group (P<0.01), and the TRIB3 protein level also showed a significant increase. These results indicated a successful construction of TRIB3-knockdown hASCs and TRIB3-overexpression hASCs. The Oil Red staining results showed that the down-regulation of TRIB3 significantly promoted lipid droplets formation in hASCs, consistent with Oil Red quantification. On the other hand, the up-regulation of TRIB3 suppressed lipid droplets formation in hASCs, consistent with Oil Red quantification. After adipogenic induction, adipogenesis-related genes, including peroxisome proliferator-activated receptor γ (PPARγ), cluster of differentiation 36 (CD36) and CCAAT/enhancer binding protein α (C/EBPα), were increased significantly in TRIB3-knockdown hASCs compared with the control group (P<0.01). Oppositely, PPARγ, CD36 and lipoprotein lipase (LPL) were significantly decreased in TRIB3-overexpression hASCs compared with the control group (P<0.01). CONCLUSION: TRIB3 inhibited the adipogenic differentiation of hASCs. Knockdown of TRIB3 promoted the ability of adipogenesis of hASCs, while overexpression of TRIB3 inhibited the adipogenic differentiation of hASCs. Considering the important role of PPARγ in the adipogenis process, the molecular mechanism of the regulatory function of TRIB3 may be related with PPARγ signal pathway.


Asunto(s)
Adipogénesis , Células Madre Mesenquimatosas , Tejido Adiposo , Células Cultivadas , Humanos
8.
Beijing Da Xue Xue Bao Yi Xue Ban ; 52(1): 107-112, 2020 Feb 18.
Artículo en Chino | MEDLINE | ID: mdl-32071472

RESUMEN

OBJECTIVE: To study the visual sensitivity threshold of physician's naked eye to the difference of nasolabial angle in edentulous jaw patients, and to provide a reference value for the study of aesthetic evaluation of soft tissue profile for the difference of nasolabial angle that can be recognized by human eyes. METHODS: Three-dimensional facial images of three edentulous patients with different diagnostic dentures introoral were obtained. Lateral screenshots of each patient's three-dimensional facial image with the same scale were obtained by using reverse engineering software (Geomagic studio 2014).The screenshot of the patient's three-dimensional facial image with suitable lip support (The suitable lip support was confirmed by both patients and prosthodontists who had clinical experience for more than 20 years) was taken as the reference picture, and the remaining pictures were grouped with it respectively. All the pictures were observed in random order by the subjects. Fifteen dentists were asked to judge the difference of nasolabial angle between the two pictures of each group on the computer screen. The difference of nasolabial angle between the two pictures in each group was measured and calculated. The ROC curve was drawn, and the best cut-off value was calculated as the visual sensitivity threshold. RESULTS: The data of the 15 subjects were used to draw ROC curves separately. The maximum and minimum best cut-off values were 5.55 degrees and 3.12 degrees respectively. The ROC curve of the whole 15 subjects was drawn after data aggregation, and the best cut-off value was 5.36 degrees (AUC=0.84>0.5, P=0.000<0.05). When the difference of nasolabial angle was above 5.36 degrees, the subjects could recognize it effectively. CONCLUSION: There is a visual limit in the observation of the nasolabial angle with the naked eye. In this study, a visual sensitivity threshold of 5.36 degrees for the difference of the nasolabial angle was obtained. The difference of nasolabial angle below this value can be regarded as no clinical significance. This result provides a reference value for human eyes to recognize the difference of nasolabial angle in soft tissue profile aesthetic evaluation. It can be applied to the aesthetic evaluation of soft tissue profile and can be used as the error level of related research with nasolabial angle as an index for accuracy evaluation.


Asunto(s)
Arcada Edéntula , Nariz , Estética , Cara , Humanos , Labio , Agudeza Visual
9.
Beijing Da Xue Xue Bao Yi Xue Ban ; 52(1): 187-192, 2020 Feb 18.
Artículo en Chino | MEDLINE | ID: mdl-32071485

RESUMEN

OBJECTIVE: To explore the possibility of the Smile Lite MDP (Smile Lite Mobile Dental Photograph) portable dental photography system applying in anterior teeth aesthetic photography, and to provide the chance to simplify aesthetic photography process and facilitate communication among patients, dentists and dental technitians. METHODS: The Smile Lite MDP photography system combined with the smartphone were used to photograph the front occlusal phase photos of anterior teeth. The photographic conditions including the color temperature parameter, the Smile Lite MDP light position and the intensity settings were explored. The best photographic conditions were chosen by blind evaluation within experienced dentists and technicians through the method of the visual analogue scale/score (VAS) evaluation, which went through statistical analysis to figure out the optimum photograghic conditions. Smile Lite MDP photography system was used to photograph the front occlusal phase photos of anterior teeth under the optimum parameter by different magnifications. The width/height ratio of the central incisor and the front width ratio of the upper anterior teeth in those photos were measured and calculated respectively. The accuracy of the anterior teeth photos taken by Smile Lite MDP photography system was analyzed based on the photographs taken by digital single lens reflex (DSLR) camera. RESULTS: The optimum color temperature parameter of Smile Lite MDP portable camera system was 5 000 K, the optimum magnification was 4 times, the best light position was the side lights of Smile Lite MDP, the optimum intensity of lights was 2 grades. Photos taken by the optimum parameters won the highest score during the VAS evaluation and the result of statistical analysis had significant difference compared with other groups (P<0.05). When comparing the photos of the upper anterior teeth taken by the Smile Lite MDP portable photography system using 4 times magnification or by DSLR using the standard parameters, there was no significant difference neither in the width/hieght ratio of the central incisor nor in the front width ratio of the upper anterior teeth (P>0.05). CONCLUSION: The Smile Lite MDP portable dental photography system applying in anterior teeth aesthetic analysis photography was capable to show the color and shape of anterior teeth in an accurate way by using the right photography parameters. Therefore, Smile Lite MDP portable dental photography system was considered to be a simple and easy photographic tool in clinical work.


Asunto(s)
Sonrisa , Estética , Estética Dental , Humanos , Incisivo , Fotograbar
10.
Beijing Da Xue Xue Bao Yi Xue Ban ; 53(1): 62-68, 2020 Dec 25.
Artículo en Chino | MEDLINE | ID: mdl-33550337

RESUMEN

OBJECTIVE: To explore the preliminary application of "biocopy function" in digital technology of temporary crown in severe tooth attrition, so as to reduce the difficulty of final restoration adjustment in severe tooth wear cases, and save clinical time. METHODS: Twelve patients aged between 40 and 65 years with severe tooth attrition were recruited in this study. The experimental group (6 cases, 3 females and 3 males) used the method of digital copy of temporary restoration shape when making the final restoration, and the control group (6 cases, 3 females and 3 males) used the traditional method to make the final restoration. The mean time of central occlusal adjustment and protrusive and lateral occlusal adjustment of each crown, the number of follow-up visits of the patients were recorded and statistically analyzed. The occlusal comfort of the first and the third months after wearing the prosthesis was evaluated by the patients, and the difference of the occlusal comfort of the final prosthesis made by the two methods was compared (the full score was 10 points). The occlusal adjustment time and occlusal comfort score were used as measurement data. Single sample t test was used. The number of follow-up visits was count data. Rank sum test was used for non-normal continuous variables. RESULTS: The mean occlusal time of each crown adjustment in the experimental group was significantly lower than that in the control group (P < 0.01), and the median number of follow-up visits in the experimental group was lower than that in the control group (P < 0.01). The average occlusal comfort score of the experimental group was higher than that of the control group at the first time (P < 0.01). After three months of crown wearing, the median occlusal comfort score of the experimental group was higher than that of the control group (P < 0.05). CONCLUSION: In the case of severe tooth attrition, it is feasible to use the "biocopy function" in the design software to design the final prosthesis, which is conducive to reduce the difficulty of adjustment and achieve predictable functional and aesthetic effects, which is of great significance to save clinical time and improve the accuracy of restoration.


Asunto(s)
Diseño Asistido por Computadora , Diente , Adulto , Anciano , Coronas , Femenino , Humanos , Masculino , Persona de Mediana Edad
11.
Beijing Da Xue Xue Bao Yi Xue Ban ; 53(1): 83-87, 2020 Dec 24.
Artículo en Chino | MEDLINE | ID: mdl-33550340

RESUMEN

OBJECTIVE: To compare the registration accuracy of three-dimensional (3D) facial scans for the design of full-arch implant supported restoration by five methods and to explore the suitable registration method. METHODS: According to the criteria, ten patients with maxillary edentulous jaw or end-stage dentition requiring implant supported restorations were enrolled in this study. A special rim with individual feature marks reflected appropriate occlusal relationship and esthetic characteristics was made for each patient. Both 3D facial scan data of natural laughter and with opener traction to expose the teeth or occlusal rim of each patient were acquired by facial scan and input to the digital analysis software Geomagic Qualify 2012. The dataset was superimposed by five different methods: seven facial anatomical landmark points alignment, facial immobile area alignment (forehead and nasal area), facial anatomical landmark points and immobile area combining alignment, facial feature points alignment, facial and intraoral feature points alignment with the same local coordinate system. The three-dimensional deviation of the same selected area was calculated, the smaller the deviation, the higher the registration accuracy. The 3D deviation was compared among the three registration methods of facial anatomical landmark points, facial immobile area alignment and the combination of the above two methods. Friedman test was performed to analyze the difference among the three methods (α=0.05). The effect of the aid of the facial and intraoral feature points were evaluated. Paired t test were performed to analyze the difference (P<0.05). RESULTS: The average three-dimensional deviation of the selected area after alignment with the facial anatomical landmarks was (1.501 2±0.406 1) mm, significantly larger than that of the facial immobile area best-fit alignment [(0.629 1±0.150 6) mm] and the combination of the two methods[(0.629 1±0.150 6) mm] (P < 0.001). The aid of the facial feature points could significantly reduce the deviation (t=1.001 3, P < 0.001). There was no significant statistical difference in the remaining groups. CONCLUSION: The forehead area of the 3D facial scan can be exposed as much as possible. The establishment of facial characteristic landmark points and the use of the invariant area alignment can improve the accuracy of registration. It should be clinically feasible to apply three-dimensional facial scan to the design of full-arch implant supported restoration with the registration of the immobile area on the face especially the forehead area.


Asunto(s)
Diseño Asistido por Computadora , Arcada Edéntula , Humanos , Imagenología Tridimensional , Arcada Edéntula/diagnóstico por imagen , Maxilar/diagnóstico por imagen , Cintigrafía
13.
J Biol Regul Homeost Agents ; 33(5): 1437-1449, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31637902

RESUMEN

Influenza has frequently been epidemic in recent years. However, the mechanisms of severe pneumonia with postinfluenza Streptococcus pneumoniae (SP) secondary infection have not been fully understood. In this study, we explored the mechanisms of pneumonia in postinfluenza A virus (IAV) infection via a mouse model. Mice were intranasally inoculated with SP three days after IAV inoculation. We then collected samples at three time points to dynamically observe the pathological progression. In IAV infection alone, lymphocyte infiltration and widened alveolar intervals were observed. In the blood, levels of the CD19+, CD19+CD21+ and CD19+CD79ß+B lymphocyte subpopulations were reduced, and IFN-γ and IL-10 were elevated. Slight atrophy was seen in the spleen, which was due to splenic B lymphocyteinitiated apoptosis through the mitochondrial pathway. When SP infection occurred after IAV infection, the pulmonary inflammation was significantly aggravated; a fair number of lymphocytes and neutrophils infiltrated simultaneously with exfoliated bronchial epithelial cells, vascular endothelial cells, widened alveolar septum and hemorrhaging. Increasing edema fluid and bacteria accumulated in the alveolar cavity. Decreased CD19+, CD19+CD21+ and CD19+CD79ß+B lymphocyte subpopulations and increased interferon gamma (IFN-γ) or interleukin 10 (IL-10) were more prominent compared to those with viral infection alone. Spleen atrophy resulting from coinfection was more obvious because of massive splenic B lymphocyte apoptosis through the mitochondrial pathway compared to viral infection alone. This study shows that although inflammation caused by SP infection alone was temporary, preceding IAV infection provided favorable conditions for SP colonization and multiplication by destroying lung structure and suppressing humoral immunity. Synergistic IAV-SP coinfection is likely to facilitate more SP colonization and promote B lymphocyte-suppression and reduction. Eventually, the pneumonia worsened.


Asunto(s)
Linfocitos B/inmunología , Infecciones por Orthomyxoviridae/inmunología , Infecciones Neumocócicas/inmunología , Neumonía Bacteriana/inmunología , Animales , Apoptosis , Linfocitos B/citología , Coinfección/microbiología , Coinfección/virología , Células Endoteliales , Virus de la Influenza A , Pulmón , Ratones , Infecciones por Orthomyxoviridae/microbiología , Infecciones Neumocócicas/virología , Streptococcus pneumoniae
14.
Artículo en Chino | MEDLINE | ID: mdl-31495108

RESUMEN

Objective: To investigate the protective effect of oligomeric proanthocyanidins (OPCs) in paraquat-exposed mice. Methods: An acute lung injury model was established by a single intraperitoneal injection of paraquat (PQ) in BALB/c mice. The mice were randomized into control group, paraquat-exposed group (PQ group) , oligomeric proanthocyanidins group (OPCs group) , and paraquat and oligomeric proanthocyanidins-exposed group (PQ+OPCs group) , with 10 mice in each group. Only normal saline was intraperitoneally injected into the mice in the control group. The mice in the PQ group were divided into 8 subgroups according to the dose of poison administered, i.e., 0, 25, 50, 75, 100, 150, 200, and 300 mg/kg; the mice in each subgroup were given a single intraperitoneal injection of PQ and were observed and recorded for death at 3, 6, 12, 24, 36, 48, 60, 84, and 96 hours after PQ injection. Origin 8.0 was used to calculate the median lethal dose (LD(50)) of the mice at 24, 36, 48, and 60 hours after PQ injection, and the PQ dose (100 mg/kg, ip) was chosen based on the accumulated mortality rate. An OPCs-treated experimental model was established by an intraperitoneal injection of OPCs followed by a single PQ injection (100 mg/kg, ip) 1 hour later to observe the effects of OPCs on the apparent poisoning effect and fatality rate in PQ-induced mice. Immunohistochemistry was used to determine the effect of OPCs on PQ-induced lung tissue lesions. The peripheral blood samples of the mice were collected to determine the effects of OPCs on PQ-induced inflammatory factors such as tumor necrosis factor-α (TNF-α) , interleukine-1ß (IL-1ß) , and transforming growth factor-ß1 (TGF-ß1) using enzyme-linked immunosorbent assay. Results: The mortality rate was significantly correlated with the dose and exposure time in PQ-exposed mice; the mortality rate gradually increased with increasing dose and exposure time of the poison (P<0.05) . The LD(50) values for the mice were 216.67, 124.11, and 71.24 mg/kg at 24, 48, and 72 hours after PQ exposure, respectively. PQ could induce animal death at 12 hours after injection, and the mortality rate of the animals was 40% (4/10) at 48 hours after PQ exposure. The PQ-induced mortality rate of the mice in the PQ+OPCs group was reduced, and the mortality rate of the animals was 10% (1/10) at 48 hours after PQ exposure. Compared with treatment in the control group, OPCs exposure alone had no significant effect on the expression of TNF-α and TGF-ß1 in the peripheral blood (P>0.05) , but it significantly inhibited the expression of IL-1ß (P<0.05) . After 48 hours, the expression of TNF-α, TGF-ß1, and IL-1ß in peripheral blood significantly increased by 39%, 45%, and 38%, respectively, in the PQ group (P<0.05) , but they significantly decreased by 31%, 13%, and 22%, respectively, in the OPCs+PQ group as compared with the PQ group (P<0.05) . Conclusion: OPCs pretreatment can significantly alleviate PQ-induced poisoning effect.


Asunto(s)
Lesión Pulmonar Aguda/tratamiento farmacológico , Paraquat/toxicidad , Proantocianidinas/farmacología , Sustancias Protectoras/farmacología , Lesión Pulmonar Aguda/inducido químicamente , Animales , Interleucina-1beta/sangre , Pulmón/efectos de los fármacos , Pulmón/patología , Ratones , Ratones Endogámicos BALB C , Distribución Aleatoria , Factor de Crecimiento Transformador beta1/sangre , Factor de Necrosis Tumoral alfa/sangre
15.
Beijing Da Xue Xue Bao Yi Xue Ban ; 51(2): 335-339, 2019 Apr 18.
Artículo en Chino | MEDLINE | ID: mdl-30996378

RESUMEN

OBJECTIVE: To explore a novel method for fabricating removable partial dentures (RPDs) using polyetheretherketone (PEEK) by computer-aided design and computer-aided manufacturing (CAD/CAM) technologies and to evaluate their fits for different assemblies in vitro. METHODS: A standard stone cast of mandibular partial edentulous jaw was scanned using a lab scanner. Based on the digital cast, thirteen complete RPDs were designed combing dental CAD software and reverse engineering software, and then fabricated using PEEK by milling machine. Fits of assemblies were evaluated quantitatively by measuring the spaces between RPDs and casts. The gaps between RPDs and stone casts in different assemblies were duplicated using light-body silicone impression material and then measured using three-dimensional (3D) digital analysis methods in virtue of a dedicated software. Statistically, one-way analysis of variance (ANOVA) was used to compare the difference of gaps among assemblies in different shapes such as occlusal rest, denture base, and major connector. Paired-samples t test was used to compare the gaps difference for the similar shape assemblies in different areas. RESULTS: One-piece PEEK RPDs were successfully designed and fabricated by CAD/CAM, and all the RPDs were well-seated on stone casts. The gaps between occlusal rests and casts [(84.3±23.6) µm] were significantly larger than those of denture bases [(32.5±27.8) µm] and major connectors [(49.9±47.0) µm], which meant that the fits of denture bases and major connectors were better than that of occlusal rests. However, the fits of all assemblies could be accepted clinically. For the similar shape assemblies in different areas, there were no significantly differences for gaps between distal extension denture bases [(25.1±55.3) µm] and non-extensive denture bases [(41.5±17.7) µm]. The gaps of occlusal rests adjacent and nonadjacent to the edentulous space were (86.1±29.8) µm and (80.8±42.1) µm respectively and there were no significantly difference between them either. These results implied that different locations had no apparent effect on the fits of assemblies. CONCLUSION: With the computer-aided design and computer-aided manufacturing technology, PEEK can be used to fabricate one-piece removable partial dentures. And all assemblies of the one-piece PEEK RPDs showed satisfying fits in vitro, indicating a promising clinical application in the future.


Asunto(s)
Dentadura Parcial Removible , Diseño Asistido por Computadora , Cetonas , Polietilenglicoles
16.
Beijing Da Xue Xue Bao Yi Xue Ban ; 51(2): 349-355, 2019 Apr 18.
Artículo en Chino | MEDLINE | ID: mdl-30996381

RESUMEN

OBJECTIVE: To design and fabricate edentulous custom trays by chair-side computer-aided design and 3-dimensional printing (CAD/3DP) system of independent research and development and to evaluate their clinical effects based on prosthodontists'satisfaction using the visual analogue scale (VAS). METHODS: Fifteen edentulous patients were randomly recruited by three prosthodontists who passed the unified operating process training. The custom trays for each patient were fabricated using both chair-side CAD/3DP system of independent development and manually conventional method, and the final impressions were made by silicone impression materials (light body). The prosthodontist's satisfaction was surveyed by the three prosthodontists via the questionnaire based on visual analogue scales. RESULTS: In the degree of the prosthodontist's satisfaction of final impressions, the average satisfactions of "try in" (the overall size, shape, convenience, etc.) on the 3DP custom tray made by independent development chair-side system and the manually conventional method were 9.18±0.19 and 8.23±0.22, respectively. The average satisfactions of the border line position (border extensions, frenal notch)were 8.91±0.40 and 7.96±0.23, respectively. The average satisfactions of the stability in border molding were 8.80±0.83 and 8.01±0.81, respectively. The average satisfactions of the quality of final impression (completeness, thickness of impression material, exposure of custom tray, shape of border) were 8.94±0.68 and 7.99±0.42, respectively. The average satisfactions of the difficulty of high quality impression (times of repetition, efficiency) were 9.20±0.37 and 7.88±0.22, respectively. The average satisfactions of the overall satisfaction were 9.11±0.49 and 7.95±0.15, respectively. There was significant difference in the satisfaction of "try in", border line position, stability in border molding, quality of final impression, difficulty of high quality impression, and overall satisfaction between the 3DP custom tray made by chair-side system of independent development and the manually conventional method (P<0.05). CONCLUSION: In the degree of the prosthodontist's satisfaction of final impression, the 3DP custom tray made by chair-side system of independent development is higher than that of the conventional custom tray, and it meets the clinical requirements and can be popularized in clinical application.


Asunto(s)
Técnica de Impresión Dental , Boca Edéntula , Diseño Asistido por Computadora , Materiales de Impresión Dental , Humanos , Impresión Tridimensional
17.
Beijing Da Xue Xue Bao Yi Xue Ban ; 51(1): 105-110, 2019 Feb 18.
Artículo en Chino | MEDLINE | ID: mdl-30773553

RESUMEN

OBJECTIVE: To establish a complete workflow of digital design and manufacturing occlusal splint for sleep bruxism, which can be preliminarily applied in clinical use, thus observe the clinical efficacy. METHODS: Twenty-four patients with sleep bruxism were recruited in the study and randomly divided into two groups by using random number tables. Digital-occlusal-splint (experimental group) treatment plan and traditional-occlusal-splint (control-group) treatment plan were carried out for each group, respectively. For experimental group, digital models of patients' both dental arches and the occlusion relationship after elevation were captured using an intraoral scanner. The occlusal splint was carried out by computer aided design/computer aided manufacturing (CAD/CAM), including splint designing and milling. For control group, the traditional soft occlusal splint was fabricated by vacuum laminator. The two kinds of occlusal splints were tried in the patients from each group, and the occlusal contacts were tested respectively by T-scan analysis system, which recorded the changes of occlusal indicators in the two groups. The retention, appearance and occlusal comfort degree were evaluated by the two groups of patients. Mann-Whitney test was performed with IBM SPSS 20.0 software, and bilateral test was performed. P<0.05 was considered to be statistically significant. RESULTS: The complete workflow of digital design and manufacturing occlusal splint was successfully established. During the clinical use, there was no statistical difference in the retention evaluation of two kinds of occlusal splints between the two groups of patients (Z=-0.538, P=0.590). The appearance score (Z=2.038, P=0.042) and the occlusal comfort score (Z=-2.579, P=0.010) of the experimental group were higher than those of the control group, with statistically significant differences. The T-scan analysis results showed that only the second molar on both sides of the traditional occlusal splint had occlusal contact in intercupsal position, while the digital occlusal splint had stable and bilaterally balanced contact between the maxillary and mandibular teeth. Furthermore, the occlusal force was uniformly distributed in the experimental group. CONCLUSION: The complete workflow of digital occlusal splint improves the occlusal design, greatly simplifies and optimizes the traditional process of making occlusal splint. This new method is resource-saving and environmental-friendly, and it is able to serve patients more conveniently and efficiently.


Asunto(s)
Ferulas Oclusales , Bruxismo del Sueño , Arco Dental , Oclusión Dental , Humanos , Flujo de Trabajo
18.
Beijing Da Xue Xue Bao Yi Xue Ban ; 51(1): 115-119, 2019 Feb 18.
Artículo en Chino | MEDLINE | ID: mdl-30773555

RESUMEN

OBJECTIVE: To establish a 3D printing system for bone tissue engineering scaffold fabrication based on the principle of fused deposition modeling, and to evaluate the controllability over macro and micro structure precision of polylactide (PLA) and polycaprolactone (PCL) scaffolds. METHODS: The system was composed of the elements mixture-I bioprinter and its supporting slicing software which generated printing control code in the G code file format. With a diameter of 0.3 mm, the nozzle of the bioprinter was controlled by a triaxial stepper motor and extruded melting material. In this study, a 10 mm×10 mm×2 mm cuboid CAD model was designed in the image ware software and saved as STL file. The file was imported into the slicing software and the internal structure was designed in a pattern of cuboid pore uniform distribution, with a layer thickness of 0.2 mm. Then the data were exported as Gcode file and ready for printing. Both polylactic acid (PLA) and polycaprolactone (PCL) filaments were used to print the cuboid parts and each material was printed 10 times repeatedly. After natural cooling, the PLA and PCL scaffolds were removed from the platform and the macro dimensions of each one were measured using a vernier caliper. Three scaffolds of each material were randomly selected and scanned by a 3D measurement laser microscope. Measurements of thediameter of struts and the size of pores both in the interlayer overlapping area and non-interlayer overlapping area were taken. RESULTS: The pores in the printed PLA and PCL scaffolds were regular and interconnected. The printed PLA scaffolds were 9.950 (0.020) mm long, 9.950 (0.003) mm wide and 1.970 (0.023) mm high, while the PCL scaffolds were 9.845 (0.025) mm long, 9.845 (0.045) mm wide and 1.950 (0.043) mm high. The struts of both the PLA and PCL parts became wider inthe interlayer overlapping area, and the former was more obvious. The difference between the designed size and the printed size was greatest in the pore size of the PLA scaffolds in interlayer overlapping area [(274.09 ± 8.35) µm)], which was 26.91 µm. However, it satisfied the requirements for research application. CONCLUSION: The self-established 3D printing system for bone tissue engineering scaffold can be used to print PLA and PCL porous scaffolds. The controllability of this system over macro and micro structure can meet the precision requirements for research application.


Asunto(s)
Impresión Tridimensional , Ingeniería de Tejidos , Andamios del Tejido , Huesos , Poliésteres , Porosidad
19.
Beijing Da Xue Xue Bao Yi Xue Ban ; 50(6): 1108-1111, 2018 Dec 18.
Artículo en Chino | MEDLINE | ID: mdl-30562791

RESUMEN

Acquired hemophilia A (AHA) is anunusual disease resulting from autoantibodies (inhibitors) against coagulation factor VIII (FVIII) and clinically manifests as bleeding, which sometimes can cause potentially limb-threatening or life-threatening situations. AHA is associated with cancers, auto-immune disorders, infections, dermatologic conditions and certain medications, among which it is commonly secondary to multiple rheumatologic conditions, such as rheumatoid arthritis, systemic lupus erythematosus (SLE), pollymyositis, autoimmune hemolytic anemia and undifferentiated connective tissue disease. In autoimmune diseases, it may be the result of autoantibody producing against FVIII, and some cases of AHA may act as the first manifestation of SLE. AHA should be suspected in patients who have spontaneously hemorrhagic events with an isolated prolonged activated partial thromboplastin time (APTT), reduced FVIII activity and a negative lupus anticoagulant (LA). When FVIII inhibitor is found, it can be diagnosed. The management of AHA focuses on the following goals: (1) controlling and preventing bleeding, (2) eradication of the inhibitor, (3) treatment of the underlying disease. Here, a case of AHA in a patient with lupus is reported. A 53-year-old man with a 4-year history of SLE developed arthralgia and ecchymotic skin lesions after arthrocentesis of knee joint. Ultrasound confirmed the presence of an intramuscular hematoma. Coagulation tests revealed that FVIII activity reduced to 1% and a prolonged APTT (92.2 s), FVIII inhibitors were found to be as high as 60.0 Bethesda Units. Initial treatments with methylprednisolone 200 mg/d were started but new hemorrhagic manifestation occurred and hisbiological indexes were not good. Then the patient was treated with intravenous pulse corticosteroids (methylprednisolone 500 mg/d), intravenous cyclophosphamide, and also plasma and prothrombin complex infusion. Subsequently, FVIII activity returned within normal ranges, FVIII inhibitors decreased and clinical improvement was significantly obtained. The patient's condition kept stable till now.Hemorrhagic events due to production of antibodies directed against coagulation factors were rarely observed in SLE and attentions should be paid to the association between SLE and AHA.Bypass treatment was considered as the immediate antihemorrhagic treatment. Corticosteroid combined with immunosuppressor was recommended as the main therapy to eradicate the inhibitors. However we still lack the therapeutic guidelines and standardized treatment in patients of AHA with SLE at present.


Asunto(s)
Hemofilia A , Lupus Eritematoso Sistémico , Autoanticuerpos , Hemofilia A/complicaciones , Humanos , Lupus Eritematoso Sistémico/complicaciones , Masculino , Persona de Mediana Edad , Tiempo de Tromboplastina Parcial
20.
Zhonghua Yi Xue Za Zhi ; 98(44): 3575-3578, 2018 Nov 27.
Artículo en Chino | MEDLINE | ID: mdl-30486572

RESUMEN

Objective: To investigate the association between thyroid nodule diameter measured by ultrasound and thyroid papillocarcinoma. Methods: A total of 389 cases undergoing thyroidectomy because of thyroid nodule between January 2016 and June 2017 in Beijing Anzhen Hospital were retrospectively reviewed. Of these patients, 170 cases had thyroid papillocarcinoma, and 219 cases had benign nodule. The nodules were divided into five groups according to their diameters: ≤1.0 cm, 1.1-2.0 cm, 2.1-3.0 cm, 3.1-4.0 cm, >4.0 cm. The prevalence of papillocarcinoma and lymphatic metastasis in each group were compared. Results: There were significant differences in age [(45.4±12.8) years vs (51.7±13.4) years, P<0.001], thyroid stimulating hormone (TSH) [1.75(1.28, 2.65) mU/L vs 1.48(0.99, 2.23) mU/L, P=0.003], thyroid-peroxidase antibody (TPOAb) [1.65(0.60, 8.40) kU/L vs 0.90(0.50, 2.40) kU/L, P=0.001], and C-reactive protein (CRP)[0.60(0.31, 1.37) mg/L vs 0.85(0.42, 1.66) mg/L, P=0.023] between thyroid papillocarcinoma and benign nodule. Of those(≤1.0 cm, 1.1-2.0 cm, 2.1-3.0 cm, 3.1-4.0 cm, >4.0 cm) five groups, the prevalence of papillocarcinoma was 72.5%, 52.5%, 29.7%, 16.1%, 2.4%, respectively(P<0.05). For papillocarcinoma patients, in diameter ≤1.0 cm and >1.0 cm groups, the prevalence of lymphatic metastasis was 29.9% and 45.8%, respectively (P=0.032). Binary logistic regression analysis showed that TSH (OR=1.350, 95%CI: 1.057-1.660, P=0.009), age(OR=0.960, 95%CI: 0.937-0.983, P=0.001) and nodule diameter (OR=0.359, 95%CI: 0.269-0.480, P=0.001) were independent associated factors of papillocarcinoma. Conclusion: As nodule diameter increased, the proportion of papillocarcinoma decreased, while the risk of lymphatic metastasis increased.


Asunto(s)
Nódulo Tiroideo , Humanos , Estudios Retrospectivos , Neoplasias de la Tiroides , Tiroidectomía , Ultrasonografía
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