The Effectiveness of Topical Dimethicone Together with a One Health Approach for the Control of Tungiasis in the Sanumás Communities, Yanomami Territory, Amazon Rainforest: A Real-World Study.

BACKGROUND: The success of tungiasis treatment is highly dependent on adequate environmental control. METHODS: This is a real-world observational cohort study designed to monitor the effectiveness of topical dimethicone together with a One Health approach for the control of tungiasis in the Sanumás communities, Amazon rainforest, Brazil. We followed up on 562 indigenous people and 81 domestic dogs for 1.5 years in a 3-month interval. A new molecular method for large-scale soil evaluation was also tested. The control of tungiasis was independently conducted by the Brazilian Ministry of Health and comprised topical dimethicone application (NYDA®) for humans, single-dose oral afoxolaner for dogs, and in-house soil fumigation with fipronil. The main outcome was the occurrence of tungiasis after the use of topical dimethicone together with the One Health approach. RESULTS: A total of 49 of the 562 indigenous people had active tungiasis at enrollment (8.72%). Only three cases of tungiasis resulted in active lesions after the use of topical dimethicone together with the One Health approach, with two cases of recurrence. From the 6-month follow-up and after, soil infestation was not detected. CONCLUSIONS: We conclude that the use of NYDA® together with animal and environmental interventions are effective measures for the control of tungiasis.

Tungiasis in the Sanumás Amerindians in the Amazon Rainforest, Brazil: Prevalence, Intensity and Morbidity.

BACKGROUND: Tungiasis is a disease associated with extreme poverty. We aimed to evaluate the prevalence of tungiasis in six different settlements of the Sanumás indigenous community in a remote area in the Auaris region, Yanomami territory, Brazil. METHODS: We conducted an observational study to detect clinical and epidemiological factors associated with tungiasis using a cross-sectional strategy and multivariate logistic regression. Soil analysis was performed by visual and microscopic methods. RESULTS: We examined 555 persons, 45 of whom had active tungiasis; 18 cases were classified as mild, 16 as moderate and 11 as severe. The disease was significantly more prevalent in children than in adults (odds ratio (OR) 15.77; 95% confidence interval (CI) = 5.34-67.91; p < 0.001). Soil infestation was significantly related to the occurrence of human tungiasis (OR = 12.29; 95% CI = 3.75-45.88). The sex and GPS location of the houses were not related to the occurrence of tungiasis. CONCLUSIONS: We conclude that tungiasis is an important problem in the Sanumás community, especially for children. We suggest that interruption of the off-host transmission cycle, together with regular treatment [human and animal interventions], must be prioritized to achieve control of tungiasis in indigenous populations.

Evolutionary analysis of JC polyomavirus in Misiones' population yields insight into the population dynamics of the early human dispersal in the Americas.

BACKGROUND: JC polyomavirus (JCV) has an ethno-geographical distribution across human populations. OBJECTIVE: Study the origins of the population of Misiones (Argentina) by using JCV as genetic marker. METHODS: Viral detection and characterization was conducted by PCR amplification and evolutionary analysis of the intergenic region sequences. RESULTS: 22 out of 121 samples were positive for JCV, including 5 viral lineages: MY (n = 8), Eu-a (n = 7), B1-c (n = 4), B1-b (n = 2) and Af2 (n = 1). MY sequences clustered within a branch of Native American origin that diverged from its Asian counterpart about 21,914 years ago (HPD 95% interval 15,383-30,177), followed by a sustained demographic expansion around 5000 years ago. CONCLUSIONS: JCV in Misiones reflects the multiethnic origin of the current population, with an important Amerindian contribution. Analysis of the MY viral lineage shows a pattern consistent with the arrival of early human migrations to the Americas and a population expansion by the pre-Columbian native societies.

Co-occurring pathogenic variants in 6q27 associated with dementia spectrum disorders in a Peruvian family.

Evidence suggests that there may be racial differences in risk factors associated with the development of Alzheimer's disease and related dementia (ADRD). We used whole-genome sequencing analysis and identified a novel combination of three pathogenic variants in the heterozygous state (UNC93A: rs7739897 and WDR27: rs61740334; rs3800544) in a Peruvian family with a strong clinical history of ADRD. Notably, the combination of these variants was present in two generations of affected individuals but absent in healthy members of the family. In silico and in vitro studies have provided insights into the pathogenicity of these variants. These studies predict that the loss of function of the mutant UNC93A and WDR27 proteins induced dramatic changes in the global transcriptomic signature of brain cells, including neurons, astrocytes, and especially pericytes and vascular smooth muscle cells, indicating that the combination of these three variants may affect the neurovascular unit. In addition, known key molecular pathways associated with dementia spectrum disorders were enriched in brain cells with low levels of UNC93A and WDR27. Our findings have thus identified a genetic risk factor for familial dementia in a Peruvian family with an Amerindian ancestral background.

Indigenous Psychology as a General Science for Escaping the Snares of Psychological Methodolatry.

Contemporary society has blurred the territorial borders that colonialism used to divide nation-states. Information about different peoples that have survived the impact of brutal violence perpetrated for centuries reaches everywhere in the world through information networks and disputes visibility. The modernization of the sciences happened during the period of consolidation of the so-called modern societies, in a process directly linked to the invasion of the indigenous territories of Abya Yala/Pindorama. Contemporary science is descendant of a large-scale colonialist process. The territory of knowledge has been colonized by economic and political interests that put researchers to work for purposes increasingly far from the desired freedom of thought. This paper argues that a escape from the entrapments of psychological methodolatry depends on the implication of a researcher connecting science to ethics, breaking the vicious cycle of reaffirmation of supposed scientific truths when they prove to be insufficient to approach basic human questions.

"Terra é vida, despejo é morte": saúde e luta Kaiowá e Guarani / "Territory is life, eviction is death": Kaiowá and Guarani health and fight / "Tierra es vida, desalojo es muerte": salud y lucha Kaiowá y Guaraní

Partindo da psicologia, este texto compõe nosso exercício ético-político de assumir a perspectiva dos povos e de suas organizações, neste caso em específico, da Aty Guasu, movimento étnico-social dos Kaiowá e Guarani de Mato Grosso do Sul. A colonização e expropriação violenta dos territórios tradicionais destes povos, o tekoha guasu, culminou na limitação do modo originário de ser, segundo os princípios cosmológicos, desdobrando-se na precarização da saúde, visível pelos altos índices de desnutrição, suicídio, violência e mortalidade. Com este estudo, visamos descrever e analisar aspectos da dimensão saúde para os Kaiowá e Guarani a partir de suas próprias narrativas. Como estratégia metodológica, realizamos a análise documental de todos os comunicados, em formato de notas, publicados entre 2011 e 2013 no blog do movimento na internet. Também participamos, de 2015 a 2020, de momentos importantes para as comunidades, como as Grandes Assembleias Kaiowá e Guarani, com registros em diário de campo. Esses procedimentos, articulados às produções teóricas da antropologia, psicologia da libertação, estudos decoloniais e anticoloniais, possibilitaram o entendimento da indissociabilidade da saúde indígena dos processos de colonização territorial e intersubjetiva. Nesse sentido, a saúde dos Kaiowá e Guarani, tendo o tekoha como aspecto vital, segundo a cosmopolítica, encontra-se em profundo conflito devido à expropriação, expulsão e confinamento empreendido pelas políticas colonialistas. Portanto, propomos a compreensão do tekoha como indicador da saúde Kaiowá e Guarani e os movimentos de reocupação dos territórios, protagonizados pela organização autônoma das comunidades e sintetizados pela sentença: "terra é vida", como retomada da saúde.(AU)

From the psychology, this text makes up our ethical-political exercise of regarding the perspective of the peoples and their organizations, in this specific case, Aty Guasu, ethnic-social movement of the Kaiowá and Guarani of Mato Grosso do Sul. Colonization and violent expropriation of the traditional territories of these peoples, the tekoha guasu, resulted in limiting the original way of being, according to cosmological principles, unfolding in the precariousness of health, visible from the high rates of malnutrition, suicide, violence, and mortality. With this research, we aim to describe and analyze aspects about the health dimension for the Kaiowá and Guarani from their own narratives. As a methodological strategy, we carry out the Document Analysis of all communications, in the form of notes, published between 2011 and 2013 on the organization's blog on the internet. We also participated, from 2015 to 2020, of important moments for the communities, such as the Highs Assemblies Kaiowá and Guarani, with records in a field diary. These proceedings, articulated with the theoretical productions of anthropology, liberation psychology, decolonial and anticolonial studies, made it possible to understand the inseparability of indigenous health from the processes of territorial and intersubjective colonization. In this sense, the health of the Kaiowá and Guarani, taking the tekoha as a vital aspect, according to cosmopolitics, is in deep conflict due to the expropriation, expulsion, and confinement undertaken by colonialist policies. Therefore, we propose the understanding of the tekoha as an indicator of Kaiowá and Guarani health, and the movements of reoccupation of territories, led by the autonomous organization of communities and synthesized by the sentence: "land is life," as a recovery of health.(AU)

Este texto realiza un ejercicio ético-político desde el aporte de la Psicología al asumir la perspectiva de los pueblos y sus organizaciones, en este caso, de la Aty Guasu, un movimiento étnico y social de los Kaiowá y Guaraní. La colonización y expropiación violenta de los territorios tradicionales de estos pueblos, los tekoa guasu, culminó en la limitación de los modos originarios de ser según los principios cosmopolíticos, que tienen como resultado la precarización de la salud, visibles por los altos índices de desnutrición, suicidio, violencia y mortalidad. En este estudio se busca tejer aproximaciones acerca la dimensión salud para los Kaiowá y Guaraní a partir de sus proprias narrativas. La metodología utilizada realizó un Análisis Documental de todos los anuncios en formato de "notas", publicados en los años 2011 y 2013, en el blog del movimiento en la internet. También hubo participación, de 2015 a 2020, en momentos importantes para las comunidades, tales como en las Gran Asambleas Kaiowá y Guaraní, con registro en diario de campo. Estos procedimientos articulados a las producciones de la Antropología, Psicología de la Liberación, Estudios Decoloniales y Anticoloniales permiten comprender la condición indisociable de la salud indígena en los procesos de colonización territorial e intersubjetiva. En este sentido, la salud de los Kaiowá y Guaraní, por el tekoha ser aspecto vital según la cosmovisión de estos pueblos, se encuentra en profundo conflicto debido a la expropiación, expulsión y confinamiento practicado por las políticas colonialistas. Por lo tanto, comprender el tekoha como indicador de la salud Kaiowá y Guaraní, y los movimientos de reocupación de los territorios, protagonizado por la autonomía de las comunidades, como recuperación de la salud, sintetiza en la sentencia: "tierra es vida".(AU)

Civilization in transition (ontological confrontation).

This paper aims at understanding the reception of the new anthropology, in particular the perspective of Eduardo Viveiros de Castro, by psychoanalysis and analytical psychology. Native peoples in Brazil have acquired visibility nationally and internationally, thanks to their mobilization and political organization. Award-winning books have been published such as A Queda do Céu (The Fall of the Sky) by Davi Kopenawa and Bruce Albert, and Ideias para Adiar o Fim do Mundo (Ideas to Postpone the End of the World) by Ailton Krenak. Freudian-based psychoanalysis keeps silent about native peoples and the new anthropology. The Lacanian psychoanalyst C. Dunker appears more open about it and wrote many articles and one important book on the subject. Jungians are the most enthusiastic and speak out politically about these peoples through courses, study groups, articles, online debates, books and the presence of native leaders and shamans in their conferences. This paper discusses the different perspectives on the issue in the psychoanalytical community, which vary from silence to excitement, and their subjacent political alliances.

Cet article vise à comprendre l'accueil par la psychanalyse et la psychologie analytique de la nouvelle anthropologie, en particulier la perspective d'Eduardo Viveiros de Castro. Au Brésil les peuples indigènes ont acquis de la visibilité au niveau national et international, grâce à leur mobilisation et leur organisation politique. Des livres ont été publiés, et ont reçu des prix, par exemple A Queda do Ceu (La Chute du Ciel) par Davi Kopenawa et Bruce Albert, et Ideias para Adiar o Fim do Mundo (Idées pour Retarder la Fin du Monde) de Ailton Krenak. La psychanalyse freudienne reste silencieuse concernant les peuples indigènes et la nouvelle anthropologie. Le psychanalyste Lacanien C. Dunker semble plus ouvert sur ce sujet et a écrit beaucoup d'articles ainsi qu'un livre important sur ces questions. Les Jungiens sont les plus enthousiastes et s'expriment politiquement concernant ces peuples par le moyen de cours, de groupes d'études, d'articles, de débats en ligne, de livres ainsi que par la présence de leaders autochtones et de chamanes dans leurs conférences. Cet article traite des différentes perspectives sur le sujet dans la communauté psychanalytique - qui vont du silence à l'excitation - et de leurs alliances politiques sous-jacentes.

El presente trabajo busca comprender la recepción de la nueva antropología, en particular la perspectiva de Eduardo Viveiros de Castro, por parte del psicoanálisis y de la psicología analítica. Las personas nativas en Brasil han adquirido visibilidad nacional e internacionalmente, gracias a su movilización y organización política. Se han publicado libros premiados como A Queda do Céu (La Caída del Cielo) de Davi Kopenawa y Bruce Albert, e Ideias para Adiar o Fim do Mundo (Ideas para posponer el Fin del Mundo) de Ailton Krenak. El psicoanálisis Freudiano hace silencio respecto a las personas Nativas y a la nueva antropología. El psicoanalista Lacaniano, C. Dunker se muestra más abierto habiendo escrito numerosos artículos y un libro importante sobre este tema. Analistas Junguianos son los más entusiastas y se pronuncian políticamente sobre estas personas a través de cursos, grupos de estudio, artículos, debates virtuales, libros y de la presencia de líderes Nativos y shamanes en sus conferencias. El presente trabajo describe las diversas perspectivas sobre el tema en la comunidad psicoanalítica, las cuales varían desde el silencio hasta el entusiasmo y las alianzas políticas subyacentes.

Este artigo tem como objetivo compreender a recepção da nova antropologia, em particular a perspectiva de Eduardo Viveiros de Castro, pela psicanálise e psicologia analítica. Os povos nativos no Brasil adquiriram visibilidade nacional e internacionalmente, graças à sua mobilização e organização política. Livros premiados foram publicados, como A Queda do Céu, de Davi Kopenawa e Bruce Albert, e Ideias para Adiar o Fim do Mundo, de Ailton Krenak. A psicanálise freudiana mantém silêncio sobre os povos nativos e a nova antropologia. O psicanalista lacaniano C. Dunker parece mais aberto sobre isso e escreveu muitos artigos e um livro importante sobre o assunto. Os junguianos são os mais entusiasmados e falam politicamente sobre esses povos através de cursos, grupos de estudo, artigos, debates on-line, livros e a presença de líderes nativos e xamãs em suas conferências. Este artigo discute as diferentes perspectivas sobre o assunto na comunidade psicanalítica, que variam de silêncio a excitação, e suas alianças políticas subjacentes.

Identification of Genomic Variants Associated with the Risk of Acute Lymphoblastic Leukemia in Native Americans from Brazilian Amazonia.

A number of genomic variants related to native American ancestry may be associated with an increased risk of developing Acute Lymphoblastic Leukemia (ALL), which means that Latin American and hispanic populations from the New World may be relatively susceptible to this disease. However, there has not yet been any comprehensive investigation of the variants associated with susceptibility to ALL in traditional Amerindian populations from Brazilian Amazonia. We investigated the exomes of the 18 principal genes associated with susceptibility to ALL in samples of 64 Amerindians from this region, including cancer-free individuals and patients with ALL. We compared the findings with the data on populations representing five continents available in the 1000 Genomes database. The variation in the allele frequencies found between the different groups was evaluated using Fisher's exact test. The analyses of the exomes of the Brazilian Amerindians identified 125 variants, seven of which were new. The comparison of the allele frequencies between the two Amerindian groups analyzed in the present study (ALL patients vs. cancer-free individuals) identified six variants (rs11515, rs2765997, rs1053454, rs8068981, rs3764342, and rs2304465) that may be associated with susceptibility to ALL. These findings contribute to the identification of genetic variants that represent a potential risk for ALL in Amazonian Amerindian populations and might favor precision oncology measures.

Perspectivism and shamanism in the Jungian clinic: the jaguar as an archetypal image of the Latin American cultural unconscious.

Amerindians, living in a perspective of synchronicity, attribute to symmetry a negative value that produces an understanding of unstable dualism cosmologies, in a continuous and dynamic imbalance, in a notion of complementarity between conscious and unconscious. These notions are in line with the view of synchronicity proposed by Jung (1952/1972) and Cambray (2013), a view that expands temporal, acausal boundaries, within a perspective of interconnection, resonance and correspondence. Amerindian epistemologies break-up the discontinuity between animals and humans. By establishing a parallel with the Jungian concept of the relationship between unconscious and conscious, we reach a dimension of personification of both, a continuous and permanent flow of meaning. We introduce the jaguar as a symbol of Amerindian cultures and as an archetypal image of the numinosum that activates the unconscious, in asymmetrical and symmetrical movements. This is a qualitative contribution of indigenous mythologies to the understanding of the relationship between unconscious and conscious. Through perspectivism and Amerindian shamanism, we reflect on the archetypal image of the jaguar, as a mythological Latin American knowledge, which contributes to an understanding of the human being in the world, in an instinctive and spiritual integration. Recognizing this cosmos expands the ability to observe and access another point of view, in which the human being is seen in the jaguar, a personification or psychification of his unconscious. In clinical practice, it means finding the humanity that was left behind by that human who became an animal. The shaman, as a therapist, takes on the role of an active interlocutor in the exchange of human and non-human subjectivities, in an amplification process.

Les Amérindiens, vivants dans une perspective de synchronicité, attribuent à la symétrie une valeur négative, qui produit une compréhension des cosmologies du dualisme instable, dans un déséquilibre continu et dynamique, avec la notion de complémentarité entre conscient et inconscient. Ces notions sont en cohérence avec la vision de synchronicité proposée par Jung (1952/1972) et Cambray (2013), une vision qui élargit les frontières temporelles et acausales, dans une perspective d'interconnexion, de résonnance et de correspondance. Les épistémologies amérindiennes cassent la discontinuité entre les animaux et les humains. En établissant un parallèle avec le concept de relation entre inconscient et conscient, nous atteignons la dimension de personnification des deux, un flux permanent de sens. Nous introduisons le jaguar en tant que symbole des cultures amérindiennes et image archétypale du numineux qui active l'inconscient, dans des mouvements asymétriques et symétriques. Il s'agit là d'une contribution qualitative des mythologies indigènes à la compréhension de la relation entre inconscient et conscient. A travers le perspectivisme et le chamanisme amérindien, nous réfléchissons à l'image archétypale du jaguar, en tant que savoir mythologique Amérindien, qui contribue à la compréhension de l'être humain dans le monde, dans une intégration instinctive et spirituelle. Reconnaitre ce cosmos élargit notre capacité à observer et à avoir accès à un autre point de vue, dans lequel l'être humain est vu dans le jaguar, une personnification ou psychification de son inconscient. Dans la pratique clinique, cela signifie de trouver l'humanité qui a été abandonnée par l'humain qui est devenu animal. Le chamane, en tant que thérapeute, prend le rôle d'un interlocuteur actif dans l'échange de subjectivités humaines et non-humaines, dans un processus d'amplification.

Los indios americanos, habitando en una perspectiva de sincronicidad, atribuyen a la simetría un valor negativo dando lugar a una comprensión sobre las cosmologías de un dualismo inestable, en un desbalance continuo y dinámico, y en una noción de complementariedad entre consciente e inconsciente. Estas nociones son similares con la mirada sobre sincronicidad propuesta por Jung (1952/1972) y por Cambray (2013), una mirada que expande las fronteras temporales, acausales, dentro de una perspectiva de interconexión, resonancia y correspondencia. Las epistemologías amerindias rompen con la discontinuidad entre animales y humanos. Al establecer un paralelismo con el concepto Junguiano de la relación entre inconsciente y consciente, alcanzamos una dimensión en la cual se personifica a ambos, en un constante y permanente fluir de sentidos. Introducimos el yaguar como símbolo de las culturas amerindias y como imagen arquetípica de lo numinoso que se activa en el inconsciente, en movimientos asimétricos y simétricos. Es una contribución cualitativa de las mitologías indígenas a la comprensión de la relación entre inconsciente y consciente. A través del perspectivismo y del shamanismo amerindio, nos proponemos reflexionar sobre la imagen arquetípica del yaguar, como conocimiento mitológico latinoamericano, el cual contribuye a una comprensión del ser humano en el mundo, en una integración instintiva y espiritual. Reconocer este cosmos expande la habilidad para observar y acceder a otro punto de vista, en el que el ser humano es visto en el yaguar, como una personificación o psiquización de su inconsciente. En la práctica clínica, significa encontrar la humanidad que fue dejada atrás por aquel humano devenido en animal. El/la shaman/a como terapeuta, toma el rol de un interlocutor activo en el intercambio de subjetividades humanas y no-humanas, en un proceso de amplificación.

Os ameríndios, vivendo em uma perspectiva de sincronicidade, atribuem à simetria um valor negativo que produz uma compreensão das cosmologias instáveis do dualismo, em um desequilíbrio contínuo e dinâmico, em uma noção de complementaridade entre consciente e inconsciente. Essas noções estão de acordo com a visão de sincronicidade proposta por Jung (1952/1972) e Cambray (2013), uma visão que expande as fronteiras temporais e acausais, dentro de uma perspectiva de interconexão, ressonância e correspondência. Epistemologias ameríndias rompem a descontinuidade entre animais e humanos. Estabelecendo um paralelo com o conceito junguiano da relação entre inconsciente e consciente, alcançamos uma dimensão de personificação de ambos, um fluxo contínuo e permanente de significado. Apresentamos a onça-pintada como um símbolo das culturas ameríndias e como uma imagem arquetípica do numinoso que ativa o inconsciente, em movimentos assimétricos e simétricos. Esta é uma contribuição qualitativa das mitologias indígenas para a compreensão da relação entre inconsciente e consciente. Através do perspectivismo e do xamanismo ameríndio, refletimos sobre a imagem arquetípica da onça-pintada, como um conhecimento mitológico latino-americano, que contribui para uma compreensão do ser humano no mundo, em uma integração instintiva e espiritual. Reconhecer esse cosmos expande a capacidade de observar e acessar outro ponto de vista, no qual o ser humano é visto na onça-pintada, uma personificação ou ou psiqueificação de seu inconsciente. Na prática clínica, significa encontrar a humanidade que foi deixada para trás por aquele humano que se tornou um animal. O xamã, como terapeuta, assume o papel de interlocutor ativo na troca de subjetividades humanas e não humanas, em um processo de amplificação.

AFA: Ancestry-specific allele frequency estimation in admixed populations: The Hispanic Community Health Study/Study of Latinos.

Allele frequency estimates in admixed populations, such as Hispanics and Latinos, rely on the sample's specific admixture composition and thus may differ between two seemingly similar populations. However, ancestry-specific allele frequencies, i.e., pertaining to the ancestral populations of an admixed group, may be particularly useful for prioritizing genetic variants for genetic discovery and personalized genomic health. We developed a method, ancestry-specific allele frequency estimation in admixed populations (AFA), to estimate the frequencies of biallelic variants in admixed populations with an unlimited number of ancestries. AFA uses maximum-likelihood estimation by modeling the conditional probability of having an allele given proportions of genetic ancestries. It can be applied using either local ancestry interval proportions encompassing the variant (local-ancestry-specific allele frequency estimations in admixed populations [LAFAs]) or global proportions of genetic ancestries (global-ancestry-specific allele frequency estimations in admixed populations [GAFAs]), which are easier to compute and are more widely available. Simulations and comparisons to existing software demonstrated the high accuracy of the method. We implemented AFA on high-quality imputed data of ∼9,000 Hispanics and Latinos from the Hispanic Community Health Study/Study of Latinos (HCHS/SOL), an understudied, admixed population with three predominant continental ancestries: Amerindian, European, and African. Comparison of the European and African estimated frequencies to the respective gnomAD frequencies demonstrated high correlations (Pearson R2 = 0.97-0.99). We provide a genome-wide dataset of the estimated ancestry-specific allele frequencies for available variants with allele frequency between 5% and 95% in at least one of the three ancestral populations. Association analysis of Amerindian-enriched variants with cardiometabolic traits identified five loci associated with lipid traits in Hispanics and Latinos, demonstrating the utility of ancestry-specific allele frequencies in admixed populations.

VNTR (CAG)n polymorphism of the ATXN2 gene and metabolic parameters of cardiovascular risk associated with the degree of obesity in the Amerindian population of Oaxaca.

INTRODUCTION: The ATXN2 gene has a VNTR (CAG)n with locus in exon1. Long alleles within the normal range (22-29 repeats) are associated with severe obesity in people from the United Kingdom, Indonesia and the Caribbean. OBJECTIVE: To analyse the influence of VNTR (CAG)n on metabolic profile in adults with obesity and pre-obesity, as well as to estimate its effect on the risk of developing diabetes. METHODS AND MATERIAL: 255 adults of Chinantec Amerindian ethnic origin were included, who underwent anthropometric and biochemical evaluation. The VNTR was amplified by end-point PCR and by 8% PAGE electrophoresis. RESULTS: Differences were found in the waist/hip circumference index and body mass index in the carriers of genotypes different to the one homozygous for 22 repeats with a Student's t-test value of 0.0041 and 0.0334, respectively. We also found an association with a family history of chronic disease. CONCLUSION: The VNTR of ATXN2 is associated with obesity in Mexican adults of Chinantec ancestry.

Population structure and forensic genetic analyses in Guarani and Kaingang Amerindian populations from Brazil.

When DNA profile comparisons between a crime scene trace and a reference sample generate correspondence, the match probability has to be estimated, so that evaluation of the strength of the forensic DNA evidence can be made. The random match probability estimations require information on allele frequencies and an adjustment factor, referred to as theta (θ) or Fst, a co-ancestry correction factor for subpopulation effects. The θ value has been standardized for urban and isolated populations, but inconsistencies have been reported when it is specifically calculated for smaller and isolated populations, including Amerindian populations. Notably, attempts to characterize forensic markers of these minor populations have been extensively limited and more conservative estimates of the correction factor may be generated for each of them. Therefore, we estimate allele frequencies of 21 autosomal STR markers used for forensic testing and calculated relevant forensic parameters for the set. In addition, we featured the possible structure of five Brazilian Amerindian populations that have been genetically isolated for centuries so we could obtain the appropriate θ value for them. The sample consisted of 319 individuals: (1) 121 Kaingang, from two communities: Ivaí (KIV=61) and Rio das Cobras (KRC=60); and (2) 198 Guaranis from three communities: Mbya from Rio das Cobras (GRC=51), Guarani Ñandeva (GND=71) and Guarani Kaiowá (GKW=76). Between Guarani populations low (Rst=0.0402, p < 10-4) to high (Rst=0.1557, p < 10-5) differentiation was found. Regarding Guarani and Kaingang populations, intermediate (Rst=0.0590, p < 10-5) to high (Rst=0.1604, p < 10-5) differentiation was found. The two Kaingang populations showed very low differentiation between them (Rst=0.0017, p = 0.27), which justifies the union of both genetic data for forensic databases and calculations. The combined power of discrimination (PD) and the combined power of exclusion (PE) were calculated for each population, demonstrating the usefulness of this set of markers in forensic and kinship analysis regarding these populations. Considering the demographic heterogeneity of Amerindian populations in general, the Fst mean value (0.03) was evaluated regarding 43 different indigenous populations from the Americas, including Guaranis and Kaingangs. This result confirms the adequacy of the standardized θ value for the forensic random match probability estimations involving Amerindian populations.

Epidemiology of chronic lymphocytic leukemia in Chilean and Amerindian population in Chile.

The study aim was to analyze incidence and presentation features of chronic lymphocytic leukemia (CLL) in Chile, in Amerindian population and in non-Native. Between 2012 and 2019, 912 patients were diagnosed, and 13 (1.4%) were Amerindian. The estimated incidence in Chilean population was 1.17/100,000 person per year, while in Amerindian, 0.09/100,000 person per year. Median age was 73 years. At diagnosis, 48, 27, and 25%, had low (0), intermediate (I/II) and high-risk (III/IV) disease on Rai classification. Diagnostic immunophenotypic Matutes score was ≥4 in 90%. Median follow-up was 37 months (range 2-87). 5-year OS was 56%, with median overall survival (OS) not reached. It was worse in men, ≥65 years, high-risk and those with increased prolymphocytes (CLL/PL). This study shows low incidence and worse OS in Chilean CLL patients, compared to those from European countries, despite similar clinical features. It also demonstrates that CLL is very uncommon in Amerindian population.

Impacts of the Goldmining and Chronic Methylmercury Exposure on the Good-Living and Mental Health of Munduruku Native Communities in the Amazon Basin.

This paper is an exploratory study that examines the illegal goldmining impacts on Munduruku communities' "Good-Living" (Xipan Jewewekukap) and explores the possible relationship between chronic methylmercury (MeHg) exposure and the worsening mental health conditions in three villages in the Middle-Tapajós River, Brazilian Amazon. The region has been experiencing a long-lasting threat of goldminers' invasions. A total of 109 people were interviewed and evaluated. Total mercury (THg) exposure levels were evaluated through hair samples analysis, from which MeHg exposure levels were calculated. The Geriatric Depression Scale-Short Form (GDS-SF) was used as a screening tool in order to assess mental health indicators. Brief non-structured interviews were carried out to investigate how goldmining is impacting the communities Good-Living. A Poisson regression model was used to estimate the possible association between mental health indicators (assessed through the GDS-SF) and the following independent variables: (i) mercury exposure level (<10.0 µg/g vs. ≥10.0 µg/g), (ii) self-reported nervousness, (iii) self-reported irritability, (iv) age group, and (v) monthly income. The analysis revealed high levels of mercury in hair samples (median: 7.4 µg/g, range 2.0-22.8; 70% and 28% of the participants had THg levels ≥6.0 and ≥10.0 µg/g, respectively) and pointed to a tendency in which higher levels of methylmercury exposure (Hg ≥ 10.0 µg/g) could be linked to worse mental health indicators. Although the GDS-SF has presented limitations due to the Munduruku sociocultural context, our findings suggest a tendency of worse mental health indicators in participants presenting high levels of MeHg exposure. Despite this limitation, the qualitative approach indicates an evident association between the impacts of goldmining and the Munduruku people's decreasing autonomy to maintain a Good-Living on their own terms, pointing to the importance of carrying out new investigations, especially considering longitudinal studies with qualitative methodologies and ethnographic approaches.

VNTR (CAG)n polymorphism of the ATXN2 gene and metabolic parameters of cardiovascular risk associated with the degree of obesity in the Amerindian population of Oaxaca. / Polimorfismo VNTR (CAG)n del gen ATXN2 y parámetros metabólicos de riesgo cardiovascular asociados con el grado de obesidad en población amerindia de Oaxaca.

INTRODUCTION: The ATXN2 gene has a VNTR (CAG)n with locus in exon1. Long alleles within the normal range (22-29 repeats) are associated with severe obesity in people from the United Kingdom, Indonesia and the Caribbean. OBJECTIVE: To analyse the influence of VNTR (CAG)n on metabolic profile in adults with obesity and pre-obesity, as well as to estimate its effect on the risk of developing diabetes. METHODS AND MATERIAL: 255 adults of Chinantec Amerindian ethnic origin were included, who underwent anthropometric and biochemical evaluation. The VNTR was amplified by end-point PCR and by 8% PAGE electrophoresis. RESULTS: Differences were found in the waist/hip circumference index and body mass index in the carriers of genotypes different to the one homozygous for 22 repeats with a Student's t test value of 0.0041 and 0.0334, respectively. We also found an association with a family history of chronic disease. CONCLUSION: The VNTR of ATXN2 is associated with obesity in Mexican adults of Chinantec ancestry.

The malaria burden of Amerindian groups of three Venezuelan states: a descriptive study based on programmatic data.

BACKGROUND: Fifty-three percent of all cases of malaria in the Americas in 2019 came from Venezuela, where the epidemic is heavily focused south of the Orinoco river, and where most of the country's Amerindian groups live. Although the disease is known to represent a significant public health problem among these populations, little epidemiological data exists on the subject. This study aims to provide information on malaria incidence, geospatial clustering, and risk factors associated to Plasmodium falciparum infection among these groups. METHODS: This is a descriptive study based on the analysis of published and unpublished programmatic data collected by Venezuelan health authorities and non-government organizations between 2014 and 2018. The Annual Parasite Index among indigenous groups (API-i) in municipalities of three states (Amazonas, Bolivar, and Sucre) were calculated and compared using the Kruskal Wallis test, risk factors for Plasmodium falciparum infection were identified via binomial logistic regression and maps were constructed to identify clusters of malaria cases among indigenous patients via Moran's I and Getis-Ord's hot spot analysis. RESULTS: 116,097 cases of malaria in Amerindian groups were registered during the study period. An increasing trend was observed between 2014 and 2016 but reverted in 2018. Malaria incidence remains higher than in 2014 and hot spots were identified in the three states, although more importantly in the south of Bolivar. Most cases (73.3%) were caused by Plasmodium vivax, but the Hoti, Yanomami, and Eñepa indigenous groups presented higher odds for infection with Plasmodium falciparum. CONCLUSION: Malaria cases among Amerindian populations increased between 2014 and 2018 and seem to have a different geographic distribution than those among the general population. These findings suggest that tailored interventions will be necessary to curb the impact of malaria transmission in these groups.

Childhood lead exposure of Amerindian communities in French Guiana: an isotopic approach to tracing sources.

In French Guiana were detected high lead (Pb) levels in blood of Amerindian people. Lead exposure is a serious hazard that can affect the cognitive and behavior development. People can be exposed to Pb through occupational and environmental sources. Fingerprinting based on stable Pb isotopes in environmental media is often used to trace natural and anthropogenic sources but is rarely paired with blood data. The objective of this study was to determine the main factors associated with high Blood Lead Levels (BLL). Soil, manioc tubers, food bowls, beverages, wild games, lead pellets and children blood were sampled in small villages along the Oyapock River. children BLL ranged between 5.7 and 35 µg dL-1, all exceeding 5 µg dL-1, the reference value proposed in epidemiologic studies for lead poisoning. Among the different dietary sources, manioc tubers and large game contained elevated Pb concentrations while manioc-based dishes were diluted. The isotopes ratios (207Pb/206Pb and 208Pb/206Pb) of children blood overlapped these of lead shots and meals. These first results confirm for the first time, the diary consumption of manioc-based food as the main contributor to Amerindian children's BLL in French Guiana, but don't exclude the occasional exposure to lead bullets by hunting activities. This is a specific health concern, since previous studies have shown that these same villagers present high levels of mercury (Hg). These communities are indeed subject to a double exposure to neurotoxic metals, Hg and Pb, both through their diet. The farming activity is based on manioc growing, and explaining that this ancestral practice can induce serious health risks for the child's development may seriously affect their food balance and cultural cohesion.

Dissecting the role of Amerindian genetic ancestry and the ApoE ε4 allele on Alzheimer disease in an admixed Peruvian population.

Alzheimer disease (AD) is the leading cause of dementia in the elderly and occurs in all ethnic and racial groups. The apolipoprotein E (ApoE) ε4 is the most significant genetic risk factor for late-onset AD and shows the strongest effect among East Asian populations followed by non-Hispanic white populations and has a relatively lower effect in African descent populations. Admixture analysis in the African American and Puerto Rican populations showed that the variation in ε4 risk is correlated with the genetic ancestral background local to the ApoE gene. Native American populations are substantially underrepresented in AD genetic studies. The Peruvian population with up to ~80 of Amerindian (AI) ancestry provides a unique opportunity to assess the role of AI ancestry in AD. In this study, we assess the effect of the ApoE ε4 allele on AD in the Peruvian population. A total of 79 AD cases and 128 unrelated cognitive healthy controls from Peruvian population were included in the study. Genome-wide genotyping was performed using the Illumina Global screening array v2.0. Global ancestry and local ancestry analyses were assessed. The effect of the ApoE ε4 allele on AD was tested using a logistic regression model by adjusting for age, gender, and population substructure (first 3 principal components). Results showed that the genetic ancestry surrounding the ApoE gene is predominantly AI (60.6%) and the ε4 allele is significantly associated with increased risk of AD in the Peruvian population (odds ratio = 5.02, confidence interval: 2.3-12.5, p-value = 2e-4). Our results showed that the risk for AD from ApoE ε4 in Peruvians is higher than we have observed in non-Hispanic white populations. Given the high admixture of AI ancestry in the Peruvian population, it suggests that the AI genetic ancestry local to the ApoE gene is contributing to a strong risk for AD in ε4 carriers. Our data also support the findings of an interaction between the genetic risk allele ApoE ε4 and the ancestral backgrounds located around the genomic region of ApoE gene.

Reduced mitochondrial DNA copy number is associated with the haplogroup, and some clinical features of breast cancer in Mexican patients.

Mitochondrial DNA (mtDNA) copy number and mitochondrial DNA haplogroups have been associated with different types of cancer, including breast cancer, because they alter cellular energy metabolism. However, whether mtDNA copy number or haplogroups are predictors of oxidative stress-related risks in human breast cancer tissue in Mexican patients remains to be determined. Using quantitative real-time PCR assays and sequencing of the mtDNA hypervariable region, analysis of mtDNA copy numbers in 82 breast cancer tissues (BCT) and matched normal adjacent tissues (NAT) was performed to determine if copy number correlated with clinical features and Amerindian haplogroups (A2, B2, B4, C1 and D1) . The results showed that the mtDNA copy number was significantly decreased in BCT compared with NAT (p = 0.010); it was significantly decreased in BCT and NAT in women > 50 years of age, compared with NAT in women < 50 years of age (p = 0.032 and p = 0.037, respectively); it was significantly decreased in NAT and BCT in the postmenopausal group and in BCT in the premenopausal group compared with NAT in the premenopausal group (p = 0.011, p = 0.010 and, p = 0.018; respectively); and it was also significantly decrease in members of the BCT group classified as having invasive ductal carcinoma I-III (IDC-I, IDC-II and IDC-III) and IDC-II for NAT compared to IDC-I of NAT (p = 0.025, p = 0.022 and p = 0.031 and p = 0.020; respectively). The mtDNA copy number for BCT from patients with haplogroup B2 was decreased compared to patients with haplogroup D1 (p = 0.01); for BCT from patients with haplogroup C1 was also decreased compare with their NAT counterpart (p = 0.006) and with BCT patients belonging to haplogroups A2 and D1 (p = 0.01 and p = 0.03; respectively). In addition, the mtDNA copy number was decrease in the sequences with three deletions relative to the rCRS at nucleotide positions A249del, A290del and A291del, or C16327T polymorphism with the same p = 0.019 for all four variants. Contrary, the copy number increased in sequences containing C16111T, G16319A or T16362C polymorphisms (p = 0.021, =0.048, and = 0.001; respectively). In conclusion, a decrease in the copy number of mtDNA in BCT compared with NAT was shown by the results, which suggests an imbalance in oxidative phosphorylation (OXPHOS) that can affect the apoptosis pathway and cancer progression. It was also observed an increase of the copy number in samples with specific polymorphisms, which may be a good sign of favourable prognosis.