Effect of Pelagic Sargassum on In Vitro Dry Matter and Organic Matter Degradation, Gas Production, and Protozoa Population.

This study determined the effect of pelagic Sargassum on in vitro dry matter and organic matter degradation, total gas production (TGP), and protozoa population. The treatments were different levels of Sargassum inclusion on a basal substrate (Stargrass hay; Cynodon nlemfuensis) as follows: T0 (control treatment based on Stargrass hay), T10 (90% Stargrass hay + 10% Sargassum), T20 (80% Stargrass hay + 20% Sargassum), and T30 (70% Stargrass hay + 30% Sargassum). Ruminal fermentation kinetics and protozoa population were determined during 72 h of in vitro incubations. Compared to control, dry matter degradability at 48 and 72 h and organic matter degradability at 24 and 48 h were higher in Sargassum treatments. TGP was lower with T20 at 48 h. The total population of protozoa and the concentration of Entodinium spp. were lower at T20 at 48 h and T30 at 72 h. Cl, S, Ca, K, and Zn (103, 5.97, 88.73, 285.70 g/kg, and 15,900 mg/kg) were high in Sargassum, reaching twice or even nine times higher than the contents in Stargrass (11.37, 1.60, 43.53, 87.73 g/kg, and 866.67 mg/kg). Overall, up to 30% pelagic Sargassum could be included in hay-based substrates from tropical grasses without negative effects on in vitro dry matter and organic matter degradability.

Evaluation of DDGS as a Low-Cost Feed Ingredient for Common Carp (Cyprinus carpio Linneus) Cultivated in a Semi-Intensive System.

Distillers dried grains with solubles (DDGS), a coproduct from the ethanol production industry, is successfully used as an ingredient in feeding cattle and pigs due to its relatively high protein and nutrient content and low price compared to cereals. The aim of this study was to establish the optimal DDGS concentration that can be included in the diet of common carp. A seven-week experiment was performed on common carp with an initial weight of 86 g feed with three experimental diets D0 (DDGS 0%), D1 (DDGS 25%) and D2 (DDGS 35%). The chemical composition of DDGS analyzed by Fourier Transform Near-Infrared (FT-NIR) spectroscopy showed a protein content of 27.56% and oil at 6.75%. Diets with DDGS did not produce significant changes in growth parameters, flesh quality, and blood biochemical profile. Regarding the oxidative status in the muscle tissue, D1 and D2 significantly reduced, in a dose-dependent manner, the specific activity of SOD and GSH, while CAT and GPX were left unaffected. In the liver tissue, CAT, GSH, MDA and carbonylated proteins were reduced in the DDGS diets. The microbiological analysis of the intestinal contents revealed a variation in microbial density depending on the diet used. The total number of aerobic germs was between 224.2 × 104 and 69.84 × 106 (D2 > D1 > D0) and the total number of anaerobic germs was between 15.2 × 102 and 28.2 × 102 (D2 > D0 > D1).

Evaluation of Fermented Oat and Black Soldier Fly Larva as Food Ingredients in Senior Dog Diets.

The aim of this study was to evaluate the suitability of fermented oat (FO) and black soldier fly larva (BSFL) as food ingredients for dogs. A total of 20 spayed female dogs were divided into four treatment groups, with 5 dogs per group. The four treatment groups consisted of a control group, a diet with 10% FO, one with 5% BSFL, and one with 10% FO and 5% BSFL, and each experimental food was fed for 12 weeks. The feeding of FO and/or BSFL did not affect the daily food intake, body weight, body condition score, fecal score, or skin condition of the dogs. In all the experimental groups, no significant differences in serum IgG, IL-10, or TNF-α levels were observed upon the feeding of FO and/or BSFL. Some hematological (white blood cell and basophils) and serum biochemical parameters (phosphorous, globulin, and alkaline phosphatase) showed significant differences with FO and/or BSFL feeding compared to the control group, but they were within the normal reference range. No adverse clinical signs related to these parameters being affected by FO and BSFL were observed. The feeding of BSFL for 12 weeks reduced the serum cholesterol level (p < 0.05) at the end of the experiment. Our findings suggest the suitability of FO and BSFL as food materials for dogs.

Corn substitution by mesquite bean flour (Prosopis juliflora) maintains growth and improves protein metabolism of Nile tilapia juveniles (Oreochromis niloticus).

The objective of this study was to evaluate the potential of mesquite bean flour (Prosopis juliflora) as an energy ingredient in extruded diets for juvenile Nile tilapia (Oreochromis niloticus). Two experiments were carried out: the first consisted of a study to evaluate the chemical composition and digestibility of the energy and nutrients of MBF; the second consisted of a growth test, in which juveniles consumed diets containing different proportions of corn substitution by MBF, in which zootechnical, hematological, physiological, and metabolic variables were evaluated. It was observed that MBF has a chemical composition similar to corn, as well as the apparent digestibility of energy and nutrients. The higher sucrose/starch ratio of the ingredient stands out, as well as the difference in digestibility (p < 0.05) between the predominant carbohydrates 87.63 and 99.25% for starch and sucrose, respectively. In the growth assay, no difference was observed between zootechnical variables (p > 0.05), and sucrase and alkaline phosphatase activities were increased (p < 0.05), which was not observed for amylase and lipase (p > 0.05). The hematological variables did not change (p > 0.05). Metabolic variables indicate a reduction in gluconeogenesis from amino acids, as can be seen by the reduction in liver transaminase levels (ALT and AST) and glutamate dehydrogenase (GDH), as well as the greater availability of free amino acids in plasmas (p < 0.05). Thus, it can be said that MBF has a high nutritional value and can totally replace corn in diets for juvenile tilapia and the metabolic findings indicate a potential protein-sparing effect.

A viewpoint on the use of microalgae as an alternative feedstuff in the context of pig and poultry feeding-a special emphasis on tropical regions.

With the current increase in meat and animal products consumption, there is a need to make production systems more sustainable. The use of microalgae in monogastric feeds, replacing widely used conventional feedstuffs such corn and soybean, can be a solution to overcome this problem. Several studies have shown promising results in the use of microalgae in feeding of both pigs and poultry. However, there are several important constraints associated to the production of microalgae. Such constraints are particularly limiting in the context of tropical regions. Research and scientific development on microalgae production systems are thus essential so that may be widely used in monogastric feeding. Herein, we conduct an overview of the major findings in the use of microalgae in the context of monogastric feeding and analyse the major constraints associated to its production and use, particularly in the specific context of tropical regions.

Effect of almond hulls as an alternative ingredient on broiler performance, nutrient digestibility, and cecal microbiota diversity.

The objective of this study was to evaluate 2 types of almond hulls (prime hulls and California-type hulls) as alternative feed ingredients for broilers. A total of 560 one-day-old Cobb male chicks were randomly placed to 7 experimental treatments with 8 replicates of 10 birds each. Seven treatments consisted of a corn-soybean meal control diet and diets containing prime hulls or California-type hulls at 3, 6, and 9%. The nitrogen-corrected true metabolizable energy, crude protein, and crude fiber from prime hulls and California-type hulls were 1,624 and 1,514 kcal/kg, 4.8 and 5.0%, and 13.1 and 26.45%, respectively. During 0-19 d of age, the inclusion of the prime hulls at 3 levels had no significant effects on growth performance, but the California-type hulls at 9% increased feed intake (P = 0.02) and feed conversion ratio (P < 0.01), compared with control. The prime hulls at 9% decreased (P < 0.01) ileal dry matter and ileal nitrogen digestibility, and the California-type hulls at 9% only decreased ileal dry matter digestibility, but both prime hulls and California-type hulls at 6% had no effects on ileal dry matter digestibility and nitrogen-corrected apparent metabolizable energy compared to control. In addition, inclusion of prime hulls at 3% decreased (P < 0.01) AMEn compared with control group. There were no significant differences in cecal microbiota diversity at a phylum or genus level among treatments, but 9% inclusion rate of the California-type hulls increased (P < 0.05) the population of certain bacteria in the genus Clostridium and Oscillospira compared with control. In conclusion, as a dietary energy and fiber source, the prime hulls can be used at up to 9% without a negative effect on body weight gain, whereas the California-type hulls can be used up to 6%.

Digestibility of spineless cactus meals in extruded diets for Nile tilapia (Oreochromis niloticus): energy, protein, amino acids, and carbohydrates.

The aim of the present study was to determine the apparent digestibility coefficients (ADCs) of gross energy, dry matter, crude protein, amino acids, and different carbohydrate fractions of spineless cactus meals (SCMs) for Nile tilapia juveniles, fed with extruded diets. Fish were fed a reference diet or one of five test diets, each of which included 70% reference diet and 30% corresponding SCM. Fecal collection was performed in modified Guelph method. The ADCs for dry matter, gross energy, and soluble carbohydrates of the "redonda" spineless cactus meal (RSCM) were higher (P < 0.05) than those of either the "IPA 20" spineless cactus meal (ISCM) or "gigante" spineless cactus meal (GSCM), and the ADCs for crude protein of the RSCM and "Orelha de elefante africana" spineless cactus meal (OEASCM) were higher than those of the other cactus meals (P < 0.05). In addition, the ADCs for dry matter, crude protein, and gross energy of the SCMs were similar to wheat middlings. The digestibility of the less soluble fraction of the evaluated carbohydrates, the non-fibrous carbohydrates, varied between 58.32 and 73.04%, indicating efficiency in the digestion and absorption process. The data obtained in this article can be the basis for growth experiments, with the substitution of traditional ingredients for SCMs. The determination of zootechnical, physiological, metabolic, and health variables can indicate the potential for using SCMs in diets for Nile tilapia.

Cafeteria-Type Feeding of Chickens Indicates a Preference for Insect (Tenebrio molitor) Larvae Meal.

This study aimed to determine whether broiler chickens display a preference for Tenebrio molitor larvae (TM) meal by evaluating ingredient acceptability and birds' performance. Sixty 14-day-old male chickens were assigned into two treatment groups (5 birds/pen, n = 6) in a cafeteria-type study: the control (C) group, and the TM group. Each pen was equipped with one bell drinker and four through feeders allocated side by side; all feeders of the C group contained a complete standard diet whereas each feeder of the TM group contained one of the following ingredients: ground corn, extruded semi-whole soybean, vitamin-mineral supplement mixture, and TM meal. Feed intake was recorded daily and growth was monitored periodically up to day 32. Chickens which had access to individual feed components showed a delay to display preference for TM, but consumed, overall, up to 50% of the total intake as TM meal. Feed intake and growth performance were lower in all periods for TM group (p < 0.02), whereas feed conversion ratio was improved on days 22-28 and days 29-32 of age (p < 0.01). Data from bivariate and multidimensional analysis indicate that birds started to reach a balance of ingredient intake at 25 days of age, showing a high correlation between consumption of each ingredient and the day of the experiment. Chickens exhibited a preference for T. molitor meal, resulting in improved feed efficiency, which allows us to conclude that it can be a suitable feed alternative for poultry.

Replacing soybean oil with increasing levels of soybean acid oil in diets of coturnix quails (Coturnix coturnix coturnix) and the effects on egg quality

ABSTRACT The effects of increasing levels of soybean acid oil in diets of quails on the quality of eggs were evaluated over a period of 56 days. A completely randomized design with five treatment levels of soybean acid oil (0, 2, 4, 6, and 8%) as a replacer for soybean oil and eight replicates of two quails each were used. A total of 240 fresh eggs were used for quality analysis, and 400 eggs were stored at 23ºC over a period of 0, 7, 14, 21, and 28 days for analysis of shelf-life. Polynomial regression and ANOVA with repeated measurements and Tukey´s tests were used. Soybean acid oil linearly increased the intensity of red (a* = -5.26 + 0.14 x, R2 = 0.89, P = 0.01) and yellow (b* = 42.32 + 0.44 x, R2 = 0.88 P = 0.01). All other variables of egg quality were not affected by the treatments, with the exception of sensory attributes, such as odor and aftertaste, which were more pronounced with soybean acid oil. In conclusion, soybean acid oil affects the intensity of yellow and red color of the yolk. Trained assessors detected differences in odor and aftertaste of eggs when soybean acid oil was included in the quail diet.

Palm kernel cake obtained from biodiesel production in diets for goats: feeding behavior and physiological parameters.

The objective of this study was to evaluate the effects of the inclusion of palm kernel (Elaeis guineensis) cake in diets for goats on feeding behaviors, rectal temperature, and cardiac and respiratory frequencies. Forty crossbred Boer male, non-castrated goats (ten animals per treatment), with an average age of 90 days and an initial body weight of 15.01 ± 1.76 kg, were used. The goats were fed Tifton 85 (Cynodon spp.) hay and palm kernel supplemented at the rates of 0, 7, 14, and 21% of dry matter (DM). The feeding behaviors (rumination, feeding, and idling times) were observed for three 24-h periods. DM and neutral detergent fiber (NDF) intake values were estimated as the difference between the total DM and NDF contents of the feed offered and the total DM and NDF contents of the orts. There was no effect of palm kernel cake inclusion in goat diets on DM intake (P > 0.05). However, palm kernel cake promoted a linear increase (P < 0.05) in NDF intake and time spent feeding and ruminating (min/day; %; period) and a linear decrease in time spent idling. Palm kernel cakes had no effects (P > 0.05) on the chewing, feeding, and rumination efficiency (DM and NDF) or on physiological variables. The use up to 21% palm kernel cake in the diet of crossbred Boer goats maintained the feeding behaviors and did not change the physiological parameters of goats; therefore, its use is recommended in the diet of these animals.

Glúten de trigo em dietas para tilápia do Nilo: digestibilidade e desempenho produtivo de larvas / Wheat gluten in the diet of Nile tilapia: digestibility and larvae growth performance

O objetivo do presente trabalho foi avaliar o potencial de utilização do glúten de trigo em dietas para tilápia-do-nilo (Orechromis niloticus), através de estudos de digestibilidade e desempenho produtivo de larvas. Para o estudo de digestibilidade foi elaborada uma dieta referência e uma dieta teste, contendo glúten de trigo. Utilizou-se o óxido de crômio III como marcador inerte. As fezes coletadas foram destinadas à análise de composição química, assim como as rações e o ingrediente teste. Além disso, foi realizada análise de leitura de óxido de crômio das fezes e das dietas. Os resultados obtidos por meio dessas análises foram utilizados para calcular os coeficientes de digestibilidade. Com base nesses coeficientes foram elaboradas as dietas experimentais dos estudos de dose-resposta para larvas. Foram formuladas cinco dietas experimentais com diferentes níveis de inclusão de glúten de trigo, sendo de 0%; 2,5%; 5,0%, 7,5% e 10%. Ao final do experimento foram avaliados o desempenho produtivo dos animais. Os dados de desempenho foram submetidos à análise de regressão linear a 5% de probabilidade. O glúten de trigo apresenta elevados coeficientes de digestibilidade para matéria seca, proteína bruta e energia bruta, sendo de 94,02; 98,18 e 96,90%, respectivamente. A análise de regressão demonstrou um efeito linear negativo quanto ao comprimento final e sobrevivência das larvas conforme aumentou-se o nível desse alimento. Dessa forma, conclui-se que o glúten de trigo é um ingrediente potencial para ser utilizado em dietas para tilápia-do-nilo, não sendo recomendada sua inclusão na fase larval.

The objective of this study was to evaluate the utilization potential of wheat gluten in diets for Nile tilapia (Oreochromis niloticus) through studies of digestibility and productive performance of larvae. For the digestibility study was elaborated a reference diet and a test diet containing wheat gluten. Chromium oxide-III was used as an inert marker. The collected faeces were destined to analysis of chemists compounds as the feed, and the test ingredient. Besides, reading chromium oxide analysis was performed in the faeces and diets. The results obtained through these analyses were used to calculate the digestibility coefficients. Based on these coefficients, experimental diets were elaborated for the dose-response studies in larvae. For the experiment, five experimental diets were formulated with different inclusion levels of wheat gluten, with 0%; 2,5%; 5,0%, 7,5% and 10%. At the end of the experiment, the productive performance was evaluated. The performance data were submitted to linear regression with 5% of probability. The wheat gluten presents high digestibility coefficients for dry matter, crude protein and gross energy with 94,02; 98.18 and 96.90%, respectively. The regression analyses showed a negative linear effect on the final length and survival of the larvae when the level of this food was increased. Thus, it is concluded that wheat gluten is a potential ingredient to be utilized in diets for Nile tilapia, not being recommended its inclusion in the larvae period.

Inclusion levels of sweet potato root meal in the diet of broilers I. Effect on performance, organ weights, and carcass quality.

The amount of corn available for animal and poultry feed has been unpredictable in recent years due to the increased use of corn for ethanol production. As a consequence, there has been an increase in the price of feed, chicken, and chicken products. Researchers are exploring alternative feed sources to substitute for corn in poultry ration. This study evaluated the performance and carcass quality of broilers fed diets containing sweet potato root meal (SPRM). After a complete nutrient analysis of the SPRM, diets were formulated where 0, 10, 20, and 30% of corn was substituted with SPRM. The study utilized 360 1-d-old Cornish X Rock male broiler chickens randomly assigned to one of 4 treatments; 0%, 10%, 20%, and 30% SPRM. Body weights and feed intake (FI) were monitored weekly for 7 wk. Birds were slaughtered on d 50 and FI, BW gain, ADG, ADFI, abdominal fat, dressing percentage, and organ weights measured. White (breast) and dark (leg and thigh) meat were evaluated for nutrient content (protein, moisture, fat, and ash). Results showed birds fed 20% SPRM had lower (P<0.03) final BW, BW gain and ADG than those fed the 30% SPRM diet. There were no differences in FI and ADFI among treatments. Feed conversion ratio was lowest (P<0.02) in birds fed 10, 20, and 30% SPRM than the control. There were no differences in dressing percentage among treatments. Abdominal fat was highest (P<0.05) in birds fed 30% SPRM. Organ weights were similar across treatments except for gizzard which weighed highest (P<0.05) in the control. For white meat; moisture, protein, fat, and ash were similar across treatments. For dark meat, moisture (P<0.004) and fat (P<0.03) were highest in the control, while protein and ash were similar among treatments. Birds fed the SPRM diets compared well with those fed the control for both performance and nutrient content of meat.

Microbiota intestinal e desempenho de frangos alimentados com rações elaboradas com sorgo ou milheto e complexo enzimático / Intestinal microflora and broiler performance fed with sorghum or pearl millet with enzymatic complexes

Avaliou-se a suplementação enzimática em rações contendo milheto ou sorgo sobre a microbiota intestinal e o desempenho de frangos. Foram alojados 420 pintos - ensaio de microbiota - e 1.200 pintos - ensaio de desempenho -, submetidos aos tratamentos com sorgo ou milheto suplementados ou não com complexo enzimático. O delineamento utilizado foi inteiramente ao acaso, com sete repetições de 15 aves cada, para o ensaio de microbiota, e em blocos ao acaso, com cinco repetições e 60 aves por parcela para desempenho. Foram realizadas pesquisas de bactérias Gram-negativas e contagem do número total de microrganismos aeróbios do intestino delgado. A suplementação enzimática não afetou a microbiota intestinal de frangos aos 14 e 28 dias de idade. Houve efeito da suplementação enzimática nas dietas com sorgo para conversão alimentar na fase pré-inicial. Nas dietas com milheto, a suplementação melhorou o ganho de peso no período de um a 35 dias de idade. No período total de criação, de um a 42 dias, não foi observado efeito da suplementação para milheto ou sorgo. Conclui-se que a utilização da suplementação de enzimas em rações com sorgo ou milheto pode melhorar os resultados de desempenho, dependendo da fase de crescimento de frangos de corte.

Two experiments were conducted to examine the supplementation enzyme in broilers fed with sorghum or pearl millet. The intestinal microflora and broiler performance was conducted with 420 and 1.200 broiler chickens, respectively. The treatments consisted of sorghum or pearl millet diet with enzymatic complex. The microflora assay consisted of 15 birds per experimental unit in completed randomized delineament, with seven replicates, and the performance study consisted of 60 birds per experimetal unit in a randomized block design, with five replicates. A study of bacteria negative-gram and total number bacteria aerobic in the small intestine was carried out. The averages were compared by contrasts (performance) and the Kruskal-Wallis (bacteria) tests applied for the research. The enzyme supplementation did not affect the broiler intestinal microflora at the age of 14 and 28 days. Feed conversion in the pearl millet diets supplemented with enzymes was better in the initial phase. The body weight was better to treat enzymatic complex in diets pearl millet (at age of 1- 35 days), however, at the age of 1 to 42 days there was no effect of supplementation for millet or sorghum. The results showed that the sorghum or pearl millet diets supplemented with enzymes are a viable strategy to improve the nutritional value of the diets and performance results depending on the age of the broilers.

Substituição de milho por sorgo baixo tanino em dietas para suínos: digestibilidade e metabolismo / Replacement of corn by low tannin sorghum in pig diets: digestibility and metabolism

Um experimento foi realizado para avaliar os efeitos digestivos e metabólicos da substituição de milho por sorgo em dietas para suínos. Foram utilizados 12 suínos machos castrados, meio-irmãos paternos, com peso vivo médio inicial de 40,8kg, mantidos em gaiolas metabólicas. O delineamento experimental foi inteiramente casualizado com três tratamentos (T1 = dieta controle; T2 = substituição de 50 por cento do milho por sorgo e T3 = substituição de 100 por cento) e quatro repetições. As digestibilidades da proteína e da energia brutas foram 5 e 4 por cento superiores (P<0,05), respectivamente, nos suínos alimentados com a dieta que tinha 50 por cento de substituição, comparadas àquela com substituição total do milho por sorgo. A substituição total do milho por sorgo reduziu (P<0,05) as digestibilidades, em relação à dieta-controle, em 3 por cento para MS, 8 por cento para PB e 5 por cento para EB. O N fecal foi 25 por cento maior (P<0,05), o N absorvido 10 por cento menor (P<0,05) e a excreção fecal de energia 15 por cento maior (P<0,05) para a dieta com substituição total, em relação à dieta-controle. A excreção fecal de N e energia e a absorção de N são influenciadas negativamente pela substituição total de milho por sorgo nas dietas. A substituição em até 50 por cento do milho por sorgo em dietas para suínos não influi na digestibilidade das dietas e no metabolismo dos animais.

An experiment was carried out to evaluate metabolic and digestive effects replacement of corn by sorghum in pig diets. Twelve half brothers barrows weighing 40.8kg and housed in metabolic cages in a completely randomized design with three treatments (T1 = control diet; T2 = replacement of 50 percent corn by sorghum and T3 = replacement of 100 percent) with four replicates. Crude protein and gross energy the digestibilities were 5 percent and 4 percent higher (P<0.05), respectively, for pigs fed 50 percent of corn replacement diet, in comparison with the diet where corn was totally replaced by sorghum. Total replacement of corn by sorghum diets decreased (P<0.05) the digestibilities, in comparison to control diet, in 3 percent for DM, 8 percent for CP and 5 percent for GE. Fecal N was 25 percent higher (P<0.05), absorbed N was 10 percent lower (P<0.05), and the fecal excretion energy was 15 percent higher (P<0.05) for total substitution diet in comparison to control diet. Fecal excretion and absorption of nitrogen are negatively influenced by total replacement of corn by sorghum in the diets. Replacements of up to 50 percent of corn by sorghum in pig diets do not affect the digestibility diet and metabolism of animals.