Perceptions of dairy cow-handling situations: A comparison of public and industry samples.

Inappropriate cattle handling poses a reputational threat to the dairy industry. To enhance social sustainability, handling practices must resonate with societal values about animal care. However, it has yet to be determined to what extent industry and public stakeholders differ in their perception of common cattle handling situations. We administered an online survey to samples of dairy industry (IND) and public (PUB) stakeholders to examine how they perceive a variety of cow-handling scenarios ranging from positive to negative in terms of effects on animal welfare. Participants were presented with 12 brief videos depicting a range of realistic cow-handling situations and responded to measures designed to assess their attitudes and beliefs about each scenario, their perception of the emotional response of the cows depicted in each scenario, as well as their own personal emotional response. Preexisting beliefs about cow treatment on US dairy farms and demographic data, including self-reported dairy consumption, were also collected and analyzed. Before viewing the videos, 52.9% of PUB (vs. 79.0% of IND) believed cows were treated well while 27.2% (vs. 9.0% of IND) believed cows were treated badly. Within IND, believing cows were treated badly was more common among nonwhites, those with greater formal education, more liberal politics, or from urban or suburban environments. In PUB, female and younger participants were more likely to believe cows were treated badly before viewing the videos. In both samples, participants with more positive preexisting beliefs about dairy cow treatment in the US reported consuming dairy products more frequently. In both PUB and IND, scenarios which were rated more positively for attitudes or for the cows' or respondents' emotional experiences were also perceived as more common. Within a given cow-handling scenario, qualitative attitudes (i.e., a positive, negative, or neutral valence) did not differ between the samples. In both samples, at the participant level, overall attitudes toward cow-handling scenarios were highly correlated with both their personal emotional response to the scenario and their perception of the cows' emotional responses. Although the participants' overall personal emotional responses did not differ between the samples, IND rated cows as experiencing more negative emotions overall. The consensus between industry and public stakeholders around dairy cow-handling practices observed in this study could provide a common starting point for addressing other, more contentious animal welfare issues.

Sheep as a Model for Liver Transplantation.

OBJECTIVE: Experimental animal liver transplantation is the initial step, before the application of the procedure on humans. Canine and swine transplantation were used to perfect the technical aspects of the procedure. Small animals such as rats were mainly utilized to study the metabolic and immunological aspects of liver transplantation. In this paper, we describe our experience with attempting liver transplantation in a sheep animal model. MATERIAL AND METHOD: The animal model used for both donor and recipient was outbred male weanling sheep of Naimi strain (Ovis aries, Awassi). They weigh between 25 and 35 kg. They were put under general anesthesia. Harvested livers were kept in cold storage. Recipients underwent hepatectomy, after construction of an active portal systemic bypass using a Medtronic pump. The implantation was done with caval replacement and direct portal anastomosis. The hepatic artery with its attachments to the aortal was anastomosed directly to the recipient aorta. RESULT: Twelve pairs (24 sheep) were utilized for donor and recipient surgery. Donor surgery was completed successfully in all 12 cases. Recipient surgery was not completed in three cases, when animals were lost in the implantation phase, before reperfusion mainly due to uncontrolled bleeding, resulting in hemodynamic instability. We also lost five recipients immediately after reperfusion, mainly due to post-perfusion bleeding and hemodynamic instability. Four recipients stayed alive after the implantation. CONCLUSION: We demonstrated the feasibility of using sheep as an animal model for liver transplantation. We described the similarities of sheep liver to that of humans, as well as the technical difficulties. This model is suitable in situations where other well-established models are not available for cultural or religious reasons. Further refinement in the technical aspects will be needed, as well as investigation of the biochemical outcome and long-term survival.

Evaluation of Chute Exit, Novelty and Human Approach Tests in Mertolenga Beef Cattle.

Livestock behavior and welfare are increasingly recognized to be related, not only to the animals' handling, but also with productivity. The present work was carried out at the Mertolenga Breed Testing Center and its objective was to evaluate the behavior of Mertolenga breed young bulls when exiting the chute, the reaction to novelty and human approach tests, as well as to understand if the responses between these tests are related. Twenty-nine Mertolenga-bred young bulls from 16 different farms, aged between 8 and 13 months, entered the study farm from the end of May to the beginning of June 2021. Data was collected on six different days and analyzed with the SAS® 9.4 software. Older animals showed a tendency to leave the chute more calmly, take longer to touch the novelty ball and to touch the ball less often, with a consequent reduction in the likelihood of playing with it. In the human approach test, animals that came out of the chute more calmly allowed the human to come closer. These behavior tests should be further studied on potential sires, so as to increase docility and manageability of autochthonous beef breeds.

Effects of different handling methods on the behavior of adult zebrafish.

The zebrafish is an important biomedical research organism. In most research, zebrafish are removed from their home tank and subsequently their phenotype is measured. The method of handling the fish, however, may significantly affect a variety of phenotypes. This is particularly problematic for studies of brain function that measure behavioral or neuronal responses. Nevertheless, the potential effects of handling have not been analyzed, and in fact are usually ignored. Here, we explore the effects of two usual and two rarely or never-before employed handling methods on the behavior of adult zebrafish. We exposed each fish to one of four handling methods, a between subject experimental design: (1) net chasing followed by air-suspension, (2) gentle net catching (without chasing) followed by air-suspension, (3) gentle net catching followed by being placed in a beaker (no chasing and very short air-suspension), (4) transportation in home tank and pouring the fish directly into the test tank (no chasing, netting or air-suspension). With these handling methods, the fish were placed in a test tank and their swim path was videorecorded and analyzed. Handling significantly affected swim path parameters, duration and frequency of immobility, absolute turn angle and its temporal variance and velocity, but not the distance to bottom. The behavioral effects confirmed that chasing and netting induce robust behavioral changes, and that pouring the fish from its home to its test tank is least aversive for zebrafish. We recommend using this latter method to reduce experimental error variation and increase reproducibility of results.

What slaughterhouse workers' attitudes and knowledge reveal about human-pig relationships during pre-slaughter operations: A profile-based approach.

Slaughterhouse workers are strategic capital for the meat industry in terms of operational and animal welfare issues; however, information about the attitudes of workers toward the human-animal relationship is limited. The main aim of our study was to identify the profiles of workers based on their attitudes toward pigs, occupational satisfaction, sociodemographics, and animal handling. The survey included 171 workers in 12 Colombian pig slaughterhouses. A factor analysis and a hierarchical cluster analysis identified four segments or worker profiles. The first comprised workers who relate to animals and their work in a mechanical way, the second comprised professional workers who are emotionally close to animals, the third comprised those committed to animals and their work, and the fourth comprised workers who are apathetic toward animals and work activity. The human-animal relationship at the slaughterhouse level is multifaceted, but is influenced by dependent on work satisfaction and sympathy toward the animals.

Benchmarking animal handling outcomes on cow-calf operations and identifying associated factors.

The assessment of animal handling is commonly included in cattle care programs. The guidelines set in the National Cattlemen's Beef Association Beef Checkoff funded Beef Quality Assurance (BQA) program are often used for assessing handling on feedlot, stocker, and cow-calf operations. There is limited information about animal handling on cow-calf operations. Thus, the objectives of this study were to: (1) quantify handling outcomes on cow-calf operations and compare them to national BQA program thresholds, and (2) investigate factors associated with handling outcomes. Researchers visited 76 operations across the United States to observe the following outcomes, adapted from the BQA program, during the processing of cows or yearling heifers: Prod Use, Miscatch, Vocalization, Jump, Slip/Stumble, Fall and Run. One hundred cows or less (depending on herd size) were observed moving through a restraint system at each operation. Other information specific to the animal type, facilities, and management were also gathered to be explored as potential predictors of handling outcomes. Data were summarized using descriptive statistics on an operation basis and analyzed with multi-predictor ANOVA or Kruskal-Wallis tests to assess the relationship between outcomes and possible explanatory factors. Predictors included in the final analyses were: BQA certification (BQA), animal temperament (TEMP), region (REGION), chute style (CHUTE), and visual contact with humans (VISUAL). The 76 operations were sampled in 24 states (Central, n = 17; East, 30; West, 29), with herd sizes ranging from 10 animals to more than 5,000 animals. A total of 4,804 animals were observed. There were a substantial number of operations exceeding BQA thresholds for Prod Use (34.0%, 26), Miscatch (46.0%, 35), and Fall (31.6%, 24); the averages of these outcomes also exceeded the BQA thresholds (< 10%, 0%, and 2%, respectively). There was an association between Prod Use and several explanatory factors, including SIZE (P = 0.072), TEMP (P = 0.001), VISUAL (P = 0.027), and BQA (P = 0.104). Miscatch, Vocalization, and Fall all had single associated factors (REGION, P = 0.019; REGION, P = 0.002; VISUAL, P = 0.002, respectively). The VISUAL and TEMP factors had an association with the majority of outcomes. The findings suggest an opportunity for improving handling outcomes, which could be achieved through education and training support regarding the importance of animal handling on-farm. Future work should consider additional aspects of facilities and management that could impact cattle handling outcomes.

Responsiveness of domesticated goats towards various stressors following long-term cognitive test exposure.

Current evidence suggests that frequent exposure to situations in which captive animals can solve cognitive tasks may have positive effects on stress responsiveness and thus on welfare. However, confounding factors often hamper the interpretation of study results. In this study, we used human-presented object-choice tests (in form of visual discrimination and reversal learning tests and a cognitive test battery), to assess the effect of long-term cognitive stimulation (44 sessions over 4-5 months) on behavioural and cardiac responses of female domestic goats in subsequent stress tests. To disentangle whether cognitive stimulation per se or the reward associated with the human-animal interaction required for testing was affecting the stress responsiveness, we conditioned three treatment groups: goats that were isolated for participation in human-presented cognitive tests and rewarded with food ('Cognitive', COG treatment), goats that were isolated as for the test exposure and rewarded with food by the experimenter without being administered the object-choice tests ('Positive', POS treatment), and goats that were isolated in the same test room but neither received a reward nor were administered the tests ('Isolation', ISO treatment). All treatment groups were subsequently tested in four stress tests: a novel arena test, a novel object test, a novel human test, and a weighing test in which goats had to enter and exit a scale cage. All treatment groups weretested at the same two research sites, each using two selection lines, namely dwarf goats, not selected for production traits, and dairy goats, selected for high productivity. Analysing the data with principal component analysis and linear mixed-effects models, we did not find evidence that cognitive testing per se (COG-POS contrast) reduces stress responsiveness of goats in subsequent stress tests. However, for dwarf goats but not for dairy goats, we found support for an effect of reward-associated human-animal interactions (POS-ISO contrast) at least for some stress test measures. Our results highlight the need to consider ontogenetic and genetic variation when assessing stress responsiveness and when interacting with goats.

Use of Micro-Computed Tomography to Visualize and Quantify COVID-19 Vaccine Efficiency in Free-Breathing Hamsters.

The SARS-CoV-2 pandemic has impacted the health of humanity after the outbreak in Hubei, China in late December 2019. Ever since, it has taken unprecedented proportions and rapidity causing over a million fatal cases. Recently, a robust Syrian golden hamster model recapitulating COVID-19 was developed in search for effective therapeutics and vaccine candidates. However, overt clinical disease symptoms were largely absent despite high levels of virus replication and associated pathology in the respiratory tract. Therefore, we used micro-computed tomography (µCT) to longitudinally visualize lung pathology and to preclinically assess candidate vaccines. µCT proved to be crucial to quantify and noninvasively monitor disease progression, to evaluate candidate vaccine efficacy, and to improve screening efforts by allowing longitudinal data without harming live animals. Here, we give a comprehensive guide on how to use low-dose high-resolution µCT to follow-up SARS-CoV-2-induced disease and test the efficacy of COVID-19 vaccine candidates in hamsters. Our approach can likewise be applied for the preclinical assessment of antiviral and anti-inflammatory drug treatments in vivo.

Evaluation of the Attitudes and Confidence of Veterinary Undergraduates toward Working with Cats in the Veterinary Hospital Environment.

An understanding of feline behavior is required to successfully handle cats in the hospital environment and avoid triggering escalation to fear-aggression due to heightened anxiety. Some veterinary students appear to misinterpret feline behavioral cues, resulting in frustration and poor success when performing basic handling. This article investigates the following in regard to veterinary students and feline handling: (a) attitudes toward working with feline patients in the hospital environment; (b) experience of working with feline patients; (c) satisfaction in acquisition of feline handling and examination skills; and, (d) confidence in feline handling skills; and to explore influencing factors. An electronic questionnaire was circulated to fourth and final year veterinary students (May 2012) and final year students (June 2013) for anonymous voluntary completion. One hundred and seventy-three of 293 students completed the questionnaire. Of final year respondents, 87/115 (75.7%) indicated that they enjoy interacting with cats in veterinary hospitals; 95/115 (82.6%) indicated that they were satisfied with their cat handling skills; 105/115 (91.3%) felt confident handling quiet and wriggly cats; significantly fewer 63/115 (54.8%) felt confident handling unpredictable cats (p < .05). Factors significantly affecting confidence in handling unpredictable cats included: frequency of examination practice during extra-mural studies (p < .05), enjoyment of interacting with cats in veterinary hospitals (p < .05), self-perceived understanding of feline behavior (p < .05), self-perceived ability to interpret cats' behavioral expressions (p < .05), satisfaction with cat handling skills (p < .05), and perception of feline versus canine handling ability (p < .05).

The use of ex-vivo liver perfusion circuit in sheep model: Preliminary report.

OBJECTIVES: To describe a novel animal model for ex-vivo liver perfusion. METHODS: This study was carried out at King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia, between September 2016 and January 2019. We assembled a perfusion circuit operated by a continuous pressure-driven arterial pump with continuous portal and arterial pressure and volume measurements. We used normothermic oxygenated perfusate. The livers used were retrieved from the sheep. RESULTS: Ex-vivo continuous perfusion of the liver was achieved for up to 9 hours with stable pressure and volume in both hepatic artery and portal vein. In 4 experiments the arterial pressure was kept in a range of 48-52 mmHg with a mean of 51.75±4.31 resulting in arterial volume at steady state of 223.5±48.25 ml/minute (95% confidence level). At steady state the mean portal pressure was 16.25±1.45 mmHg with a mean volume of 854±313.75 ml/minute (95% confidence level). Bile production was observed during the perfusion period. Hemodynamic parameters were similar to the physiological parameters observed in normothermic perfusion model of the porcine liver. CONCLUSION: A normothermic oxygenated ex-vivo perfusion circuit was successfully constructed using the sheep liver. A sustainable functional circuit with physiological hemodynamic parameters was achieved. Further study on sheep model seems to be feasible.

The Youngest, the Heaviest and/or the Darkest? Selection Potentialities and Determinants of Leadership in Canarian Dromedary Camels.

Several idiosyncratic and genetically correlated traits are known to extensively influence leadership in both domestic and wild species. For minor livestock such as camels, however, this type of behavior remains loosely defined and approached only for sex-mixed herds. The interest in knowing those animal-dependent variables that make an individual more likely to emerge as a leader in a single-sex camel herd has its basis in the sex-separated breeding of Canarian dromedary camels for utilitarian purposes. By means of an ordinal logistic regression, it was found that younger, gelded animals may perform better when eliciting the joining of mates, assuming that they were castrated just before reaching sexual maturity and once they were initiated in the pertinent domestication protocol for their lifetime functionality. The higher the body weight, the significantly (p < 0.05) higher the score in the hierarchical rank when leading group movements, although this relationship appeared to be inverse for the other considered zoometric indexes. Camels with darker and substantially depigmented coats were also significantly (p < 0.05) found to be the main initiators. Routine intraherd management and leisure tourism will be thus improved in efficiency and security through the identification and selection of the best leader camels.

Occupational Injuries among Latino/a Immigrant Cattle Feedyard Workers in the Central States Region of the United States.

Agriculture is a dangerous industry with high rates of occupational injuries. Immigrants comprise the majority of the hired agricultural workforce in the United States, and these workers may be at a higher risk for job-related injuries. This study addressed the frequency, characteristics, and risk factors of occupational injuries among Latino immigrant cattle feedyard workers. Data were collected through structured interviews with Latino immigrant cattle feedyard workers in Kansas and Nebraska (n = 243; 90.9% male). Descriptive statistics and logistic regression were used to identify risk factors for injury. Nearly three-fourths of participants (71.2%) reported having experienced one or more injuries in the past while working on a cattle feedyard. The most frequent types of reported injuries, including those not requiring medical care, were bruises/contusions (40%), cuts/lacerations (21%), and sprains/strains (12%). These injuries were mainly caused by animals/livestock (33%), chemicals (23%), falls (12%), and tools (9%). Significant risk factors for injury included male gender (OR 5.9), being over age 35 (OR 2.6), working on a large or an extra-large feedyard (OR 5.4), having 11 or more employees on the feedyard (OR 3.6), and working more than eight hours a day (OR 4.7). Having received safety training was also associated with greater risk of injury in a univariable model (OR 2.6). Cattle feedyard workers are at high risk for injury and require more effective preventive measures.

Improved Trapping and Handling of an Arboreal, Montane Mammal: Red Panda Ailurus fulgens.

It is sometimes essential to have an animal in the hand to study some of their ecological and biological characteristics. However, capturing a solitary, cryptic, elusive arboreal species such as the red panda in the wild is challenging. We developed and successfully tested a protocol for tracking, trapping, immobilization, and handling of red pandas in the wild in eastern Nepal. We established a red panda sighting rate of 0.89 panda/day with a capture success rate of 0.6. We trapped and collared one animal in 3.7 days. On average, we took nearly 136 (range 50-317) min to capture an animal after spotting it. Further processing was completed in 38.5 (21-70) min. Before capture, we found it difficult to recognize the sex of the red panda and to differentiate sub-adults above six months from adults. However, body weight, body length, tail length, shoulder height, and chest girth can be used for diagnosis, as these attributes are smaller in sub-adults. Our method is a welfare-friendly way of trapping and handling wild red pandas. We report new morphometric data that could serve as a guide for field identification.

Teaching Gentle Canine and Feline Handling as a Veterinary Clinical Skill.

This article was migrated. The article was marked as recommended. Gentle animal handling techniques decrease the fear, anxiety, and stress (FAS) felt by companion animals during veterinary visits. These techniques, relatively new in the veterinary field, can be taught to veterinary students in a progressive clinical skills curriculum using models and live animals. This article includes a series of comprehensive lesson plans that are simple to adopt and easy to modify to fit the needs of individual institutions teaching these skills. These laboratories, each reviewing and building on content previously presented, are meant to be accompanied with specific feedback offered by instructors overseeing student performance of skills. Students' deliberate practice of these techniques is meant to build, refine, and reinforce gentle animal handling from the start of their veterinary education to prepare them to handle animals using these techniques during their clinical training and beyond.

Human Behaviour Change Interventions in Animal Care and Interactive Settings: A Review and Framework for Design and Evaluation.

Behaviour change interventions may be one of the most promising avenues to improve animal welfare. Yet there has been limited systematic research involving them in animal-related settings. We searched three major databases for studies involving an intervention to change interactive or care-related behaviours in any animal-related setting. Forty-seven papers were included in the review and each paper was coded for specific design and evaluation elements. We found a series of limitations in the quality and consistency of intervention design, evaluation, and reporting. Hence, we present a framework, the "Ten-Task" guide, based on the intervention mapping framework, to guide future work in this field. Adopting this structured approach will improve the quality and efficacy of behaviour change interventions for animal welfare and allow for the field to progress in a harmonious way.

Animal handling and stress-related behaviour at mobile slaughter of cattle.

By avoiding animal transportation, mobile slaughter may have the potential to reduce animal stress. In a cross-sectional study with elements of cohort design, we investigated relationships between animal handling and stress-related animal behaviours in connection with slaughter at two Swedish slaughter plants: a newly started small-scale on-farm mobile abattoir and a relatively large-scale stationary slaughterhouse. To the stationary plant, the animals were transported on average 99 km from farms, and one third of these animals spent one night in lairage before slaughter. Data were collected during processing of 298 animals at both plants during one year. Stockperson actions and animal behaviours were observed in the driveways (2.4-7.3 m long) to the stun box. Data on season, hour of day, air temperature, animal breed, animal category, animal age, carcass weight and stockperson category (plant or farm) were also collected. We used Spearman rank correlation, principal-component analysis and confirmatory factor analysis to develop a final generalised structural equation model, which contained six variables that represented observed animal backing; turning; slipping; eliminating; vocalising; and violent behaviour, regressed on a latent variable representing animal stress level. Stress level and animal behaviours were also regressed on five variables representing observed stockperson actions (touching, patting or hitting with hand; touching, patting or hitting with a tool; pushing; tail-twisting; and prodding with an electric goad) and on background variables including plant identity. The animal behaviours were modelled as counts per driveway length, clustered on farm identity. Marginal effects of stockperson actions and predictive margins of plants were calculated. The animals displayed backing and violent behaviour (kicking, goring or violent fighting) at significantly lower frequencies at the mobile plant than at the stationary one. In general, stockperson actions were significantly positively associated with animal behaviours, i.e. increased actions were associated with more frequent behaviours. Stockperson moving with tool, pushing and using electric goad were indirectly significantly associated with all animal behaviours via animal stress level. This study shows the importance of adequate cattle handling to limit pre-slaughter stress.

Predicting career sector intent and the theory of planned behaviour: survey findings from Australian veterinary science students.

BACKGROUND: Producing graduates for a breadth of sectors is a priority for veterinary science programs. Undergraduate career intentions represent de-facto 'outcome' measures of admissions policy and curricula design, as intentions are strong predictors of eventual behaviour. Informed by Ajzen's Theory of Planned Behaviour, this study aimed to identify if contextually relevant attitudes and self-ratings affect student intentions for veterinary career sectors. RESULTS: Survey responses from 844 students enrolled in five Australian veterinary programs in 2014 were analysed. Intention was measured for biomedical research/academia, industry, laboratory animal medicine, public health/government/diagnostic laboratory services, mixed practice, intensive animal production, companion animal practice, not work in the veterinary profession, and business/entrepreneurship. Hierarchical multiple linear regression analysis enabled comparison of explanation of variance in intent by demographics, animal handling experience, species preference, and attitudes to aspects of veterinary work. Career sector intentions were highest for mixed or companion animal clinical practice, then business/entrepreneurship, then non-clinical sectors. Overall, intent was explained to a greater extent by species preferences than by animal experience, attitudes to aspects of veterinary work and demographics (with the exception of mixed practice intent) with gender having no significant effect. Several variables exerted negative effects on career intent for less popular career sectors. CONCLUSION: Ajzen's Theory of Planned Behaviour (TPB) provides a framework to increase understanding of and predict career sector intentions. Incorporation of attitude and self-efficacy measures in our study revealed preference for species types contributes greatly to career sector intentions for veterinary students, particularly for the more popular practice based sectors. Importantly, specific species preferences and other attitudes can have a negative effect on intent for non-aligned veterinary sectors. Further research is required to identify additional attitudes and/or beliefs to better explain variance in intent for less popular career sectors. Veterinary admissions processes may benefit from utilising the TPB framework. Identified effects revealed by this study may stimulate innovation in marketing, recruitment, admissions and curricular design, such as timing and role modelling, to utilise positive effects and mitigate against negative effects identified for sectors requiring greater representation of career intent in the student body.

Standardization of Small Animal Imaging-Current Status and Future Prospects.

The benefit of small animal imaging is directly linked to the validity and reliability of the collected data. If the data (regardless of the modality used) are not reproducible and/or reliable, then the outcome of the data is rather questionable. Therefore, standardization of the use of small animal imaging equipment, as well as of animal handling in general, is of paramount importance. In a recent paper, guidance for efficient small animal imaging quality control was offered and discussed, among others, the use of phantoms in setting up a quality control program (Osborne et al. 2016). The same phantoms can be used to standardize image quality parameters for multi-center studies or multi-scanners within center studies. In animal experiments, the additional complexity due to animal handling needs to be addressed to ensure standardized imaging procedures. In this review, we will address the current status of standardization in preclinical imaging, as well as potential benefits from increased levels of standardization.

Motivation and Prior Animal Experience of Newly Enrolled Veterinary Nursing Students at two Irish Third-Level Institutions.

Veterinary nurses report an intrinsic desire to work with animals. However, this motivation may be eroded by poor working conditions and low pay, resulting in the exit of experienced veterinary nurses from clinical practice. This study sought to quantify the level of animal-handling experience students possessed at the start of their training and to explore the factors motivating them to enter veterinary nurse training in two Irish third-level institutions. The authors had noted a tendency for veterinary nursing students to possess limited animal-handling skills, despite their obvious motivation to work with animals. The study explores possible reasons for this, as it mirrors previous reports in relation to students of veterinary medicine. First-year veterinary nursing students at Dundalk Institute of Technology and University College Dublin were surveyed and a focus group was held in each institution to explore student motivations for choosing this career and their prior animal-handling experience and workplace exposure. The results show that veterinary nursing students are highly intrinsically motivated to work with and care for animals. The majority had spent time in the veterinary workplace before starting their studies but they had limited animal-handling experience beyond that of family pets, primarily dogs. The study also revealed potential tensions between the veterinary nursing and veterinary medical students at University College Dublin: a hitherto unexposed aspect of the hidden curriculum in this institution. The results of this study highlight the need for ongoing investment in practical animal-handling training for veterinary nursing students.