RESUMEN
Artabotrys pilosus (Annonaceae) is endemic to China, this plant has high medicinal value and broad application prospect. In this study, we assembled and systematically analyzed the chloroplast genome of A. pilosus on the basis of DNA sequencing using high-throughput techniques. The chloroplast sequence of A. pilosus was 178,195 bp in length, including two inverted repeat regions of 42,150 bp, a large single-copy region of 90,797 bp and a small single-copy region of 3098 bp. It was predicted to contain 142 genes, of which 96 are coding, 38 are tRNA genes, and eight are rRNA genes. The overall GC content was 38.8%; this was higher in the IRs (40.4%) when compared to the LSC (37.6%) and the SSC (32%) regions. Phylogenetic analysis showed that A. pilosus is in subfamily Annonoideae.
RESUMEN
Objective: To study the chemical constituents from twigs and leaves of Artabotrys pilosus. Methods: The chemical constituents of A. pilosus were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were determined by physicochemical properties, spectral data, as well as comparisons with the data in literature. Results: Sixteen compounds were isolated from the acetic ether fraction of 90% ethanol extract from the twigs and leaves of A. pilosus, and identified as dammaradienylacetate (1), lupeol (2), lupenyl acetate (3), betulinic acid (4), uvaol (5), ursolic acid (6), β-amyrin (7), erythrodiol (8), friedelinol (9), friedelin (10), α-spinasterone (11), stigmast-7-ene-3β-ol (12), stigmast-5-ene-3β-ol-7-one (13), stigmastan-3,6-diketone (14), stigmasterol (15), and β-sitosterol (16). Conclusion: All compounds are isolated from A. pilosus for the first time. Except for compounds 15 and 16, other compounds are isolated from the plants of Artabotrys R. Br. for the first time.