Breaking Biomphalaria black box by in situ revelation of fluorescent Schistosoma mansoni parasites.

Tissue clearing is an old-fashioned method developed in the 1900's and used to turn an opaque biological object into a 3D visualizable transparent structure. Developed and diversified over the last decade, this method is most of the time applied to mammals' tissues, and especially mouse and human tissues for cytological, histological and pathophysiological studies. Through autofluorescence, immunofluorescence, in situ hybridization, intercalating agents, fluorescent transfection markers or fluorescent particle uptake, optically cleared samples can be monitored to discover new biological structures and cellular interactions through 3D-visualization, which can be more challenging in some extend through classical histological methods. Most of the tissue clearing procedures have been developed for specific applications like endogenous fluorescence visualization, immunolabeling or for revealing specific organs. Thus, choosing the adapted protocol may be empirical for non-model species, especially for mollusks for which very little related literature is available. Herein, we suggest an effective optical tissue clearing procedure for the freshwater snail Biomphalaria glabrata, known as the intermediate host of the human parasite Schistosoma mansoni. This clearing procedure involves solvents with a minimal toxicity, preserves the endogenous fluorescence of labeled parasites inside snail tissues and is compatible with an immunolabeling procedure.

AI-based Cleft Lip and Palate Surgical Information is Preferred by Both Plastic Surgeons and Patients in a Blind Comparison.

INTRODUCTION: The application of artificial intelligence (AI) in healthcare has expanded in recent years, and these tools such as ChatGPT to generate patient-facing information have garnered particular interest. Online cleft lip and palate (CL/P) surgical information supplied by academic/professional (A/P) sources was therefore evaluated against ChatGPT regarding accuracy, comprehensiveness, and clarity. METHODS: 11 plastic and reconstructive surgeons and 29 non-medical individuals blindly compared responses written by ChatGPT or A/P sources to 30 frequently asked CL/P surgery questions. Surgeons indicated preference, determined accuracy, and scored comprehensiveness and clarity. Non-medical individuals indicated preference. Calculations of readability scores were determined using seven readability formulas. Statistical analysis of CL/P surgical online information was performed using paired t-tests. RESULTS: Surgeons, 60.88% of the time, blindly preferred material generated by ChatGPT over A/P sources. Additionally, surgeons consistently indicated that ChatGPT-generated material was more comprehensive and had greater clarity. No significant difference was found between ChatGPT and resources provided by professional organizations in terms of accuracy. Among individuals with no medical background, ChatGPT-generated materials were preferred 60.46% of the time. For materials from both ChatGPT and A/P sources, readability scores surpassed advised levels for patient proficiency across seven readability formulas. CONCLUSION: As the prominence of ChatGPT-based language tools rises in the healthcare space, potential applications of the tools should be assessed by experts against existing high-quality sources. Our results indicate that ChatGPT is capable of producing high-quality material in terms of accuracy, comprehensiveness, and clarity preferred by both plastic surgeons and individuals with no medical background.

Management of Tongue-Tie Using Conventional Technique for Speech Clarity and Correcting Malocclusion: A Case Report.

Ankyloglossia, which is a congenital condition also referred to as tongue-tie, is described as a small lingual frenum that restricts tongue movement and its function. The main purpose of the frenum is to restrict the movement of the cheek, lip muscles, and tongue throughout the development of fetuses and maintain equilibrium between the developing bones, lip musculature, and tongue. The constriction of the buccal musculature counteracts the outward pressure that the tongue applies to the teeth. Arch width maintenance requires a state of equilibrium between these two muscle groups. Therefore, altering tongue position might additionally have an impact on a mandible's position. A 20-year-old female patient presented to the Periodontics Department with moderate ankyloglossia (Kotlow Class II). For the correction of the tongue-tie, conventional surgery with sutures was scheduled under local anesthesia. One week, one month, and three months follow-up, the patient demonstrated good healing. There was an improvement in speech clarity and tongue movements.

Drinking water safety improvement and future challenge of lakes and reservoirs.

To meet the Sustainable Development Goal (SDG) target 6.1, China has undertaken significant initiatives to address the uneven distribution of water resources and to enhance water quality. Since 2000, China has invested heavily in the water infrastructure of numerous reservoirs, with a total storage capacity increase of 4.704 × 1011 m3 (an increase of 90.8%). These reservoirs have significantly enhanced the available freshwater resources for drinking water. Concurrently, efforts to improve water quality in lakes and reservoirs, facilitated by nationwide water quality monitoring, have been successful. As a result, an increasing lakes and reservoirs are designated as centralized drinking water sources (CDWSs) in China. Among the 3,441 CDWSs across all provinces, 40.8% are sourced from lakes and reservoirs, 32.6% from rivers, and 26.6% from groundwater in 2023. Notably, from 2016 to 2023, the percentage of lakes and reservoirs categorized as CDWSs has increased consistently across all 29 provinces. This progress has enabled 561.4 million urban residents to access improved drinking water sources in 2022, compared to 303.4 million in 2004. Our findings underscore the pivotal role of water infrastructure construction and water quality improvement jointly promoting lakes and reservoirs as vital drinking water sources. Nevertheless, the nationwide occurrence of algal blooms has surged by 113.7% from the 2000s to the 2010s , which is a considerable challenge to drinking water safety. Fortunately, algal blooms have been markedly alleviated in past four years. However, it is still crucial to acknowledge that lakes and reservoirs face the challenges of algal blooms, and associated toxic microcystin and odor compounds.

The Effects of an Accelerated Auditory Matching Protocol for Early Intervention Students.

We employed a multiple probe design to test the effects of an accelerated auditory matching protocol with five toddlers receiving early intervention services (four males, one female) for developmental delays. All participants emitted poorly articulated vocal mands and tacts. The dependent variables were the number of full echoics, partial echoics, and incorrect responses within a set of 20 two-syllable words. The independent variable was an accelerated Auditory Matching Protocol that targets auditory discrimination using an iPad App. Each phase of the protocol targets different sound discriminations with growing complexity. Four out of five participants emitted more full or partial echoic responses upon mastery of the Auditory Matching Protocol. Moreover, compared to those who received the full dosage of the standard Auditory Matching Protocol in previous studies, the participants in this study required fewer learn units to master all phases and to demonstrate improvement. Supplementary Information: The online version contains supplementary material available at 10.1007/s40617-023-00882-1.

Linking undergraduates' future orientation and their employability confidence: The role of vocational identity clarity and internship effectiveness.

Given the growing research interest in students' school-to-work transition, we examine the positive effects of undergraduates' future orientation on their employability confidence. Specifically, building on social cognitive career theory which suggests that career choices are influenced by a person's experiences, beliefs, interests, and values, we explore the mediator of vocational identity clarity and the positive moderator of internship effectiveness in this association above. Two studies were conducted to test our model. Study 1 included a laboratory experiment (N = 136) and tested the positive association between undergraduates' future orientation and their employability confidence through vocational identity clarity. Study 2 used a time-lagged research design from undergraduates (N = 315) to replicate the results and to additionally test a mediating role of internship effectiveness. We find that undergraduates' future orientation has a strong positive relationship with employability confidence, and this relation is mediated by vocational identity clarity. Additionally, the results show a positive moderation effect, that is, when undergraduates' internship effectiveness is high, the above indirect relation becomes stronger. The current research enriches the existing understanding of how and when the students' future orientation can contribute to employability confidence theoretically and empirically. Meanwhile, these findings indicated valuable practical applications by encouraging universities to promote undergraduates' future orientation and provide effective internships for them towards enhancing their vocational identity clarity and ultimately boosting their employability confidence.

Using benefit transfer to estimate housing value increases from improved water clarity: A case study of lakes in Kosciusko County, Indiana.

This study provides step-by-step guidance for practitioners and local stakeholders on how to use existing study results to conduct benefit transfer (BT), and ultimately make informed predictions of how improvements in lake water clarity may benefit surrounding communities. The procedures are demonstrated using a publicly available meta-dataset developed by the United States Environmental Protection Agency, and a subsequent meta-analysis that synthesizes the literature on how improvements in water clarity impact home values. The BT procedures are demonstrated using a case study of 14 large lakes in Kosciusko County, Indiana. Lake-specific average increases in home values, as well as the value of the housing stock in aggregate, are calculated for illustrative improvements in lake water clarity. This analysis provides a critical bridge to better connect high-quality, academic research with real-world policy analysis, and ultimately serves to better equip local governments and stakeholders to make more informed policy and land use decisions.

Automatic imitation is modulated by stimulus clarity but not by animacy.

Observing actions evokes an automatic imitative response that activates mechanisms required to execute these actions. Automatic imitation is measured using the Stimulus Response Compatibility (SRC) task, which presents participants with compatible and incompatible prompt-distractor pairs. Automatic imitation, or the compatibility effect, is the difference in response times (RTs) between incompatible and compatible trials. Past results suggest that an action's animacy affects automatic imitation: human-produced actions evoke larger effects than computer-generated actions. However, it appears that animacy effects occur mostly when non-human stimuli are less complex or less clear. Theoretical accounts make conflicting predictions regarding both stimulus manipulations. We conducted two SRC experiments that presented participants with an animacy manipulation (human and computer-generated stimuli, Experiment 1) and a clarity manipulation (stimuli with varying visual clarity using Gaussian blurring, Experiments 1 and 2) to tease apart effect of these manipulations. Participants in Experiment 1 responded slower for incompatible than for compatible trials, showing a compatibility effect. Experiment 1 found a null effect of animacy, but stimuli with lower visual clarity evoked smaller compatibility effects. Experiment 2 modulated clarity in five steps and reports decreasing compatibility effects for stimuli with lower clarity. Clarity, but not animacy, therefore affected automatic imitation, and theoretical implications and future directions are considered.

Reliability and Validity of Ecological Momentary Assessment Response Time-Based Measures of Emotional Clarity: Secondary Data Analysis.

BACKGROUND: Emotional clarity has often been assessed with self-report measures, but efforts have also been made to measure it passively, which has advantages such as avoiding potential inaccuracy in responses stemming from social desirability bias or poor insight into emotional clarity. Response times (RTs) to emotion items administered in ecological momentary assessments (EMAs) may be an indirect indicator of emotional clarity. Another proposed indicator is the drift rate parameter, which assumes that, aside from how fast a person responds to emotion items, the measurement of emotional clarity also requires the consideration of how careful participants were in providing responses. OBJECTIVE: This paper aims to examine the reliability and validity of RTs and drift rate parameters from EMA emotion items as indicators of individual differences in emotional clarity. METHODS: Secondary data analysis was conducted on data from 196 adults with type 1 diabetes who completed a 2-week EMA study involving the completion of 5 to 6 surveys daily. If lower RTs and higher drift rates (from EMA emotion items) were indicators of emotional clarity, we hypothesized that greater levels (ie, higher clarity) should be associated with greater life satisfaction; lower levels of neuroticism, depression, anxiety, and diabetes distress; and fewer difficulties with emotion regulation. Because prior literature suggested emotional clarity could be valence specific, EMA items for negative affect (NA) and positive affect were examined separately. RESULTS: Reliability of the proposed indicators of emotional clarity was acceptable with a small number of EMA prompts (ie, 4 to 7 prompts in total or 1 to 2 days of EMA surveys). Consistent with expectations, the average drift rate of NA items across multiple EMAs had expected associations with other measures, such as correlations of r=-0.27 (P<.001) with depression symptoms, r=-0.27 (P=.001) with anxiety symptoms, r=-0.15 (P=.03) with emotion regulation difficulties, and r=0.63 (P<.001) with RTs to NA items. People with a higher NA drift rate responded faster to NA emotion items, had greater subjective well-being (eg, fewer depression symptoms), and had fewer difficulties with overall emotion regulation, which are all aligned with the expectation for an emotional clarity measure. Contrary to expectations, the validities of average RTs to NA items, the drift rate of positive affect items, and RTs to positive affect items were not strongly supported by our results. CONCLUSIONS: Study findings provided initial support for the validity of NA drift rate as an indicator of emotional clarity but not for that of other RT-based clarity measures. Evidence was preliminary because the sample size was not sufficient to detect small but potentially meaningful correlations, as the sample size of the diabetes EMA study was chosen for other more primary research questions. Further research on passive emotional clarity measures is needed.

Improving the diagnosis of urinary tract infections by the use of enriched media and a 48-hour incubation period.

Introduction. The absence of a gold-standard methodology for the microbiological diagnosis of urinary tract infections (UTI) has led to insufficient standardization of criteria for the interpretation of results and processing methods, particularly incubation time and culture media.Hypothesis. 48-hour incubation time period and use of blood agar enhances the sensitivity of microorganisms isolated significantly.Aim. To determine the sensitivity of blood agar and Brilliance UTI chromogenic agar, incubating for different periods (24-48 hours), for the detection of positive urine cultures.Methodoloy. Comparisons were made between all possible combinations of media and incubation times. As the gold-standard reference, we used the routine methodology of our laboratory, which involves prior screening with available clinical data, flow cytometry, sediment analysis and/or Gram staining. Screened samples were then cultured on blood agar and chromogenic agar and incubated for 48 hours. Also, based on the results of Gram staining, additional media were added in selected cases.Results. The most significant difference was found between chromogenic agar incubated for 24 hours and blood agar incubated for 48 hours, with the latter method allowing the recovery of 10.14 % more microorganisms (P < 0.0001). Furthermore, the value of performing Gram staining to guide processing was demonstrated, as it avoided the loss of at least 5.14 % of isolates.Conclusions. At least in urological and nephrological patients it is essential to include enriched culture media (blood agar) or to extend the incubation times due to the improvement of the diagnostic sensitivity of urine cultures. Gram staining also can help detect the presence of fastidious microorganisms or mixed infections, indicating whether rich and/or selective media should be included to enhance the diagnostic sensitivity of cultures. If this methodology is not followed, it should be noted that besides fastidious species, fastidious strains of Escherichia coli, Proteus mirabilis, Pseudomonas aerugniosa and Stenotrophomonas maltophilia will also be missed.

Crystalline lens clarity in patients treated with isotretinoin.

CLINICAL RELEVANCE: Isotretinoin has been the best treatment option for moderate and severe acne vulgaris since the 1980s. Some studies have shown evidence of subclinical anterior segment involvement of the eye in patients treated with isotretinoin. BACKGROUND: This study aimed to evaluate lens clarity with the densitometry software of Scheimpflug tomography in patients treated with isotretinoin and to compare with healthy control subjects. METHODS: Thirty-seven acnepatients treated with isotretinoin who met the inclusion criteria (24 males and 13 females, mean age 22.94 ± 4.21 years) and 39 healthy control subjects were included in the study. Clinical characteristics of the isotretinoin and control subjects were recorded. Lens density was evaluated with the densitometry software of the Scheimpflug tomography device (PentacamHR, Oculus, Wetzlar, Germany). RESULTS: There was no statistically significant difference between the groups in age, gender distribution, spherical equivalent, or anterior segment parameters measured by the Pentacam system (p > 0.05 for all). Lens density values in zones 2 and 3 were significantly higher in the isotretinoin group (p = 0.042, p < 0.001) and positively correlated with cumulative isotretinoin dose (zone 2: r = 0.384, p = 0.032; zone 3: r = 0.384, p = 0.005). CONCLUSION: Zone 2 and zone 3 lens density are higher in patients treated with isotretinoin when compared to healthy controls.

Use of automated quality assessment algorithms in fingermark detection research - Application to IND/Zn vs DFO.

When developing detection techniques for fingermarks, the detected fingermarks must be evaluated for their quality to assess the effectiveness of the new method. It is a common practice to compare the performance of the new (optimized) technique with the traditional or well-established ones. In current practice, this evaluation step is carried out by a group of human assessors. A new approach is applied in this paper and consists of using algorithms to perform this task. To implement this approach, the comparison between IND/Zn and DFO has been chosen because it has already been the subject of many articles published in recent years and a consensus exists on the superiority of IND/Zn over DFO. The quality of 3'600 fingermarks developed using both detection techniques was assessed automatically using two algorithms: LQM (Latent Quality Metric) and ILFQM (Improved Latent Fingerprint Quality Metric). The distribution of quality scores was studied for both detection techniques. The results showed that fingermarks detected with IND/Zn received higher scores on average than fingermarks detected with DFO, which is in line with the consensus in the literature based on human assessment. The results of this research are promising and shows that automated fingermark quality assessment is an efficient and viable way to comparatively assess fingermark detection techniques.

Letter to the editor on "in vitro & in silico study of hypoglycaemic potential of pterocarpus marsupium heartwood extract".

The Short Communication "In vitro & in silico study of hypoglycemic potential of Pterocarpus marsupium heartwood extract" published in Natural Product Research is praised for its depth of inquiry and exploration of hypoglycemic and antioxidant properties. However, there are areas for refinement, such as clearer definitions of key terms related to type 2 diabetes mellitus, updated literature review, improved organization and contextualization of the Results and Discussion section, and a more balanced discussion of the study's implications.

Clarity tubes as effective citizen science tools for monitoring wastewater treatment works and rivers.

Improved freshwater resource management requires the implementation of widespread, effective, and timely water quality monitoring. Conventional monitoring methods are often inhibited by financial, infrastructural, and human capacity limitations, especially in developing regions. This study aimed to validate the citizen-scientist-operated transparency or clarity tube (hereafter "clarity tube") for measuring water clarity as a proxy for total suspended solids (TSS) concentration, a critical quality metric in river systems and wastewater treatment works (WWTW) effluent in Southern Africa. Clarity tubes provided a relatively accurate and precise proxy for TSS in riverine lotic systems and WWTW effluent, revealing significant inverse log-linear relationships between clarity and TSS with r2 = 0.715 and 0.503, respectively. We demonstrate that clarity-derived estimates of TSS concentration (TSScde) can be used to estimate WWTW compliance with WWTW effluent TSS concentration regulations. The measurements can then be used to engage with WWTW management, potentially affecting WWTW performance. Overall, these findings demonstrate the usefulness of clarity tubes as low-cost, accessible, and easy-to-use citizen science tools for high spatial and temporal resolution water quality monitoring, not only in rivers in Southern Africa but also in WWTW effluent for estimating compliance, with strong global relevance to the sustainable development goals (SDGs). Integr Environ Assess Manag 2024;00:1-10. © 2024 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

Cost-effectiveness of erosion mitigation to meet water clarity targets in the Manawatu-Whanganui Region of New Zealand.

Soil erosion is a significant environmental issue worldwide. It affects water quality, biodiversity, and land productivity. New Zealand government agencies and regional councils work to mitigate soil erosion through policies, management programmes, and funding for soil conservation projects. Information about cost-effectiveness is crucial for planning, targeting, and implementing erosion mitigation to achieve improvements in sediment-related water quality. While there is a good understanding of the costs of erosion mitigation measures, there is a dearth of literature on their cost-effectiveness in reducing sediment loads and improving water quality at the catchment level. In this study, we estimate the cost-effectiveness of erosion mitigation measures in meeting visual water clarity targets. The analysis utilizes the spatially explicit SedNetNZ erosion process and sediment budget modelling in the Manawatu-Whanganui Region and region-specific mitigation costs. The erosion mitigation measures considered in the analysis include afforestation, bush retirement, riparian retirement, space-planted trees, and gully tree planting. We modelled two scenarios with on-farm erosion mitigation implemented across the region from 2021 to 2100, resulting in a 48% and 60% reduction of total sediment load. We estimate the marginal costs to achieve the visual national bottom line for water clarity, as assessed by the length of waterways that meet the clarity targets. We also estimate the marginal costs of improving average water clarity, which can be linked with non-market valuation studies when conducting a cost-benefit analysis. We find that gully tree planting and space-planted trees are the most cost-effective mitigation measures and that riparian retirement is the least cost-effective. Moreover, cost-effectiveness is highly dependent on current land use and the biophysical features of the landscape. Our estimates can be used in cost-benefit analysis to plan and prioritize soil erosion mitigation at the catchment and regional levels.

Learning in the workplace: Development of a simple language statement assessment tool that supports second-level nurse practice.

AIM: To focus learning through clarity of the enrolled nurse (EN) role (a second tier nurse position) through development of a user-friendly workplace performance assessment tool commensurate with EN standards for practice. BACKGROUND: Internationally, the nursing workforce comprises regulated and unregulated staff. In Australia, similar to other western countries, there are two tiers of regulated workforce, namely Registered Nurses (RNs) and Enrolled Nurses (ENs). Differences in RN and EN standards based on the education preparation are not always clearly differentiated in workplace practice. Roles are often seen as interchangeable: Improved clarity of both regulated and unregulated roles, when numbers of healthcare workers are burgeoning, assists performance assessment that guides further learning and safe practice. DESIGN: Two phase sequential, non-experimental design. METHODS: Phase one used focus groups (n=48), expert reference panel (n=8) and end-users (n=16) to develop simple language statements. Phase two involved field testing of the statements. FINDINGS: A 30-item, criterion-based workplace performance tool was developed. Principal component analysis of completed tools indicated work could be organised around three key areas of practice, namely, higher order thinking and problem solving, routine daily activities of care and personal and social attributes. DISCUSSION: Participants reported the statement items assisted in determining suitable activities and accompanying cues in discussing learning needs. Analysis assisted with discriminating broader elements of EN workplace performance. CONCLUSIONS: Workplace learning is important for nurses to continue to build their capacity to deliver optimum care. Assessment tools that describe professional capability in plain language statements and provide examples of supportive behavioural cues help guide on-going learning through improving the validity and thereby consistency of assessment processes. Furthermore, comprehensible and meaningful statements and cues can readily be adopted by students and educators to target learning and feedback thereby enhancing clarity of the EN role, to distinguish from other nursing roles.

Emotional Clarity and Psychopathology in Women Who Have Experienced Physical Intimate Partner Violence.

Intimate partner violence (IPV) is a chronic, traumatic stressor related to posttraumatic stress (PTS), depression, and anxiety. As psychological symptoms are exacerbated in those with poor emotional clarity, the present study evaluates the relationship between emotional clarity and PTS, dissociation, depression, and worry in women who experienced at least one instance of physical IPV (n = 88). Hierarchical regression analyses, controlling for childhood trauma, IPV abuse severity, and IPV-related brain injury, found that lack of emotional clarity was significantly related to greater PTS, dissociative experiences, depression, and worry. Results suggest that emotional clarity may be a relevant therapeutic target for individuals with a history of IPV and psychological distress.

The Reciprocal Relations between Parental Psychological Control and Social Anxiety and the Mediating Role of Self-Concept Clarity Among Chinese Early Adolescents.

Although parental psychological control has been well-documented as a significant predictor of social anxiety among adolescents, few studies examine how changes in parental psychological control and adolescent social anxiety are reciprocally related at the within-person level, especially in Chinese culture. This longitudinal study examined reciprocal relations between parental psychological control and social anxiety, and the potential mediating role of self-concept clarity, by disentangling between- and within-person effects. A total of 4731 students (44.9% girls; Mage = 10.91 years, SD = 0.72) participated in a four-wave longitudinal study with 6-month intervals. Results from random intercept cross-lagged panel modeling indicated that parental psychological control directly predicted social anxiety, and vice versa. Parental psychological control indirectly predicted social anxiety via self-concept clarity, and social anxiety also indirectly predicted parental psychological control via self-concept clarity. These findings reveal a vicious cycle of mutual influence between parental psychological control and adolescent social anxiety in Chinese youth, and highlight the crucial role of self-concept clarity in the interplay between parenting and adolescent social functioning.

Understanding the Effect of External and Internal Assets on Hazardous/Harmful Drinking Among Chinese College Students: A Serial Mediation Model.

Hazardous/harmful drinking among college students is increasingly becoming a global health concern. Previous studies have mostly paid more attention to the prevalence and risk factors of alcohol use disorder based on the public health approach, and less is understood about psychological mechanisms and protective factors of problem drinking behavior. Grounded in the framework of developmental assets, this study explored the predictive effect of external assets (i.e., social connectedness) and internal assets (i.e., self-concept clarity and drinking refusal self-efficacy) on hazardous/harmful drinking among Chinese college students. Participants were 598 college students with a mean age of 19.41 (SD = 1.21) years. The results of structural equation modeling showed that social connectedness negatively predicted college students' hazardous/harmful drinking. Moreover, our path analyses revealed a serial mediation among these variables: students with high social connectedness tended to report high self-concept clarity, which in turn increased drinking refusal self-efficacy, and consequently decreased the level of hazardous/harmful drinking. This study revealed the mechanisms underlying how external and internal assets predict risky drinking among college students. These findings made significant contributions to prevention and precision interventions for college-aged youth with drinking problems.

Daily associations between global self-esteem and self-concept clarity and their relationships with subjective well-being in a sample of adult workers.

OBJECTIVES: The present pre-registered study examined the reciprocal day-to-day associations between global self-esteem and self-concept clarity and their incremental validity with respect to daily life satisfaction, positive affect, and negative affect. METHODS: We used intensive longitudinal data from 153 adult workers (45.1% women), over a period of 31 days. Data were analyzed using dynamic structural equation modeling. RESULTS: Results attested higher global self-esteem and self-concept clarity mean levels for older vs. younger participants, and lower global self-esteem and self-concept clarity variability for older vs. younger participants. Furthermore, global self-esteem and self-concept clarity were correlated at a cross-sectional daily level, yet only self-concept clarity states positively predicted subsequent global self-esteem states, while global self-esteem states did not predict subsequent self-concept clarity states. Daily global self-esteem and daily self-concept clarity further predicted subsequent daily higher life satisfaction and positive affect, respectively. CONCLUSION: Overall, our findings shed light on the short-term relationships linking global self-esteem and self-concept clarity, pointing to their discriminant validity in predicting individuals' subjective well-being.