RESUMEN
RESUMEN Objetivo: Contextualizar una receta médica de comienzos del siglo XIX en el Nuevo Reino de Granada, en la cual se prescribe el guaco para disminuir síntomas generados por afecciones del sistema músculo-esquelético. De igual forma, analizar, en función de los conocimientos actuales, cómo actúa la fórmula sobre mecanismos fisiopatológicos de la enfermedad, explicando la reducción del dolor y las secuelas asociadas. Material y método: Búsqueda documental en el Archivo Histórico Cipriano Rodríguez Santamaría, de la Biblioteca Octavio Arizmendi Posada, de la Universidad de La Sabana. Se analizó el documento denominado Reumatismo. Posteriormente se realizó una revisión de la literatura entre 1999-2018, en las bases de datos ScienceDirect/ClinicalKey/Scielo. Conclusiones: Existe evidencia científica que podría explicar la efectividad del guaco, usado en el Nuevo Reino de Granada por sus propiedades antiinflamatorias y analgésicas aportadas por componentes como la cumarina y los flavonoides. Sin embargo, una descripción vaga en la posología del guaco, signos, síntomas y comorbilidades que no se mencionan en la receta, dificulta analizar la eficacia del tratamiento y cómo lograba disminuir o controlar específicamente los síntomas dados por afecciones del sistema musculoesquelético con su aplicación. Esta tradición, en consecuencia, carece de sustento propiamente científico para el tratamiento médico de enfermedades osteomusculares.
ABSTRACT Objective: To contextualize a medical prescription of the early 19th century in the New Kingdom of Granada, in which guaco was prescribed to reduce symptoms caused by musculoskeletal system disorders, which were ill-defined at the time. Similarly, based on current knowledge, to analyse the manner in which the formula acts on pathophysiological mechanisms of rheumatic diseases, in order to explain the reduction of pain, and associated sequelae. Material and method: Documentary research into the Cipriano Rodríguez Santamaría Historical Archive of the Octavio Arizmendi Library of the University of La Sabana, in Chía, Colombia. The document analysed was called Rheumatism. Subsequently, a review of the literature was carried out in Science Direct / Clinical Key / Scielo databases in the period from 1999 to 2018. Conclusions: There is scientific evidence that supports the efficiency of guaco used in the Kingdom of New Granada due its anti-inflammatory and analgesic properties. However, a vague description of the dosage of the guaco, signs, symptoms, and comorbidities, which are not mentioned in the prescription, hinders the understanding of its application and the thorough effectiveness of the treatment in order to control the symptoms of musculoskeletal system conditions. This tradition, consequently, lacks proper scientific support for the medical treatment of musculoskeletal disorders.
Asunto(s)
Humanos , Enfermedades Reumáticas , Mikania , Sistema Musculoesquelético , Terapéutica , ConocimientoRESUMEN
It is important to study the stability of plant extracts used as active ingredients in phytotherapic medicine, as degradation of the active principles directly affects the efficacy and safety of these products. Therefore, a stability study of the hydroalcoholic extract of the species: Mikania glomerata and Mikania laevigata was conducted in order to determine the speed of degradation and shelf life of these extracts, which are incorporated in cough syrup in Brazil. Leaves of both species were dried in an oven or by lyophilization (freeze-dried). Hydroalcoholic extracts underwent both accelerated stability study of six months and long-term stability study for 12 months. Samples were stored at different temperatures and every three months were analysed by ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) to monitor their chemical profile, quantifying coumarin and chlorogenic acid. For all conditions of the study, a reduction of the content of the chemical marker of this species, coumarin, greater than 5% was observed, so a shelf life of two years cannot be assigned to the hydroalcoholic extracts of these species as observed in commercial extracts.
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Extractos Vegetales/análisis , Eficacia , Asteraceae/clasificación , Mikania/clasificación , Espectrometría de Masas/métodos , Ácido Clorogénico/efectos adversos , Cromatografía Líquida de Alta Presión/métodos , Tos , Cumarinas/clasificaciónRESUMEN
ETHNOPHARMACOLOGICAL RELEVANCE: Mikania glomerata Spreng. (MG) and Mikania laevigata Sch. Bip. ex Baker (ML), popularly known as guaco, are medicinal plants similar in morphology, chemical composition and medicinal uses. Both species are often used and sold without distinction; however, it is believed that their chemical composition is different. AIM: Thus, the aim of this study is to investigate if the aqueous extract of MG and ML present similar anti-inflammatory activity to the point of being used interchangeably. MATERIAL AND METHODS: Different doses of both extracts and coumarin were given to rats in different experimental models to assess the anti-inflammatory activity between these two species. For this, the animals were submitted to paw edema, pleurisy and degranulation of peritoneal mast cell and the extracts were also characterized by Ultra High Efficiency Liquid Chromatography coupled to Mass Spectrometry (UHPLC-MS). RESULTS: The chromatographic method showed that ML presents ten times more coumarin than MG. Oral administration of MG, ML and coumarin inhibited paw edema induced by carrageenan (400â¯mg/kg, 55% inhibition; 400â¯mg/kg, 57% inhibition; 75â¯mg/kg, 38% inhibition; pâ¯<â¯0.05, respectively). MG, ML and coumarin treatment also inhibited the edema induced by compound 48/80 (400â¯mg/kg, 56% inhibition; 400â¯mg/kg, 69% inhibition; 75â¯mg/kg, 40% inhibition; pâ¯<â¯0.05, respectively). MG, ML and coumarin did not prevent mast cell degranulation and the consequent histamine release in Wistar rat peritoneal mast cells induced by compound 48/80. MG did not inhibit cell infiltration in pleurisy nor the highest dose tested, while ML decreased the leukocyte migration (200 and 400â¯mg/kg, 23% and 30% inhibition; pâ¯<â¯0.001, respectively) and, to a lesser extent, coumarin also reduced cell infiltration (10, 50 and 75â¯mg/kg; 15%, 16% and 17% inhibition; pâ¯<â¯0.001, respectively). CONCLUSION: The variation of the results of the anti-inflammatory activity found in M. glomerata and M. laevigata demonstrates that these two species should not be used interchangeably. Coumarin, as already proven, has anti-inflammatory action however, we have suggested that it probably is not the only component responsible for this therapeutic effect in the extracts.
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Antiinflamatorios/uso terapéutico , Edema/tratamiento farmacológico , Mikania , Extractos Vegetales/uso terapéutico , Pleuresia/tratamiento farmacológico , Animales , Antiinflamatorios/química , Antiinflamatorios/farmacología , Carragenina , Degranulación de la Célula/efectos de los fármacos , Edema/inducido químicamente , Edema/inmunología , Masculino , Mastocitos/efectos de los fármacos , Mastocitos/fisiología , Mikania/química , Fitoquímicos/análisis , Fitoquímicos/farmacología , Fitoquímicos/uso terapéutico , Extractos Vegetales/química , Extractos Vegetales/farmacología , Hojas de la Planta/química , Pleuresia/inducido químicamente , Pleuresia/inmunología , Ratas Wistar , p-Metoxi-N-metilfenetilaminaRESUMEN
Introdução: Nos últimos anos ocorreu o aumento de casos relacionados com a infecção por Candida spp. e Staphylococcus spp., bem como o aparecimento de cepas resistentes a antibióticos convencionais. A biossíntese de nanopartículas consiste na redução de um íon metálico por compostos de origem natural como metabólitos secundários de plantas e organismos, sendo a forma mais indicada por apresentar menor toxicidade quando comparada à síntese química. Desta forma, a síntese biológica constitui uma alternativa para a obtenção de novos agentes ativos para o tratamento de infecções microbianas. Objetivos: Sintetizar nanopartículas de prata a partir do extrato aquoso de Mikania glomerata Sprengel e avaliar possível atividade microbicida e citotóxica. Material e Métodos: Para a síntese das nanopartículas de prata (AgNPs) foi utilizado um extrato aquoso das folhas de M. glomerata e uma solução de nitrato de prata. As AgNPs sintetizadas foram avaliadas por espectrofotômetro UV-vis e espectrometria de absorção atômica com chama. Além disso, a atividade antimicrobiana foi avaliada contra cepas de Candida albicans e Staphylococcus aureus e atividade citotóxica contra linhagens celulares HeLa e Vero. Resultados: As AgNPs são mais eficientes no combate à linhagem de Candida albicans e Staphylococcus aureus quando comparadas ao extrato puro administrado. Até a concentração de 100 mg/mL do extrato puro não foi observado efeito inibitório em ambos os micro-organismos. Entretanto quando em contato com as AgNPs, a concentração inibitória foi de 0,006 mg/mL e 0,1 mg/mL para S. aureus e C. albicans, respectivamente. O efeito citotóxico nas células se comportou de maneira dose-dependente, apresentando maior potencial citotóxico contra a linhagem celular cancerosa HeLa. Conclusão: As AgNPs sintetizadas apresentaram potencial antimicrobiano contra C. albicans e S. aureus, além de baixa atividade contra células normais, indicando sua confiabilidade para aplicação das AgNPs como forma alternativa de tratamento. Estes resultados são promissores e contribuem para pesquisa relacionada à produção de medicamentos utilizando extrato de plantas e metais.
Introduction: In recent years there has been an increase in cases related to infection by Candidaspp. and Staphylococcus spp., as well as the appearance of strains resistant to conventional antibiotics. Nanoparticle biosynthesis consists of the reduction of a metal ion by compounds of natural origin as secondary metabolites of plants and organisms, being the most indicated form because it presents less toxicity when compared to the chemical synthesis. In this way, the biological synthesis is an alternative to obtain new active agents for the treatment of microbial infections. Objective: Synthesize silver nanoparticles from the aqueous extract of Mikania glomerata Sprengel and evaluate possible microbicidal and cytotoxic activity. Material and Methods: For the synthesis of the silver nanoparticles (AgNPs) an aqueous extract of the leaves of Mikania glomerata plus a solution of silver nitrate was used. AgNPs synthesized was evaluated by UV-vis spectrophotometer and FAAS. Furthermore, antimicrobial activity was evaluated against strains of Candida albicans and Staphylococcus aureus and cytotoxicity activity against HeLa and Vero cell lines. Results: AgNPs are shown to be more efficient in combating Candida albicans and Staphylococcus aureusstrains when compared to the pure administered extract. Up to the concentration of 100 mg/mL of the pure aqueous extract no inhibitory effect was observed on both microorganisms. However when the strains were in contact with AgNPs, the inhibitory concentration was 0.006 mg/mL and 0.1 mg/mL for S. aureus and C. albicans, respectively. The cytotoxic effect on the cells behaves in a dose-dependent manner, presenting greater cytotoxic potential against the HeLa cancer cell line. Conclusion: Thus, these results are promising and contribute to research related to the production of drugs using plant extract and metals. The AgNPs synthesized presented the antimicrobial potential against C. albicans and S. aureus, in addition to low activity against normal cells, indicating their reliability for application of AgNPs as an alternative form of treatment.
Asunto(s)
Staphylococcus aureus/efectos de los fármacos , Candida albicans/efectos de los fármacos , Nanopartículas del Metal/uso terapéutico , Antiinfecciosos/uso terapéuticoRESUMEN
Herbal medicine is an ancient practice that has been gaining acceptance of the medical class through scientific studies that prove its effectiveness. However, its use should still be cautious. Medicinal plants have potential toxic effects not yet discovered, and may have unproven interactions with other medications. The use of drugs during pregnancy is still very dangerous and vigorously studied; however, there are few studies of herbal medicines in pregnant women. Existing studies prioritize on teratogenic or abortifacient effects. The aim of this study was to analyze the toxic effects of Mikania glomerata Sprengel administration, popularly known as "guaco" during the gestational period of hypertensive rats. For this experimental groups consisting of pregnant Wistar rats received treatments with guaco extract (1 to 2 mL). In order to analyze the possible toxic effects of guaco during pregnancy, weight gain of rats was assessed during pregnancy; reproductive performance of rats, morphological parameters, and fetal placental histology were compared. Although some parameters presented significant differences, we can conclude that changes prioritized by literature, such as toxicity, vasodilation and hypotension, have not been caused by guaco. The only fetal changes observed were due to the maternal hypertension. Some studies have reported vasodilator and hypotensive effects of guaco. However, only a few studies exist, and its actual effects remain unknown. Specific studies should be developed with higher doses of guaco for a definitive conclusion of its toxic and non-toxic effects.
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Embora seja notável o avanço no uso popular de plantas com finalidade terapêutica, estudos são necessários para demonstrar seus potenciais tóxicos, já que grande parte dos usuários de fitoterápicos os considera inócuos. Durante a gestação, a maioria das alterações no feto é decorrente da exposição da mãe a agentes químicos, podendo ocorrer efeitos abortivos, teratogênicos e citotóxicos. A relevância deste trabalho se deve a escassez de estudos sobre os efeitos tóxicos do extrato de Mikania glomerata, popularmente conhecida como guaco, durante o período gestacional. Foram estudadas ratas Wistar prenhes (normotensas e espontaneamente hipertensas) sob tratamento com extrato seco de M. glomerata (2mL) e avaliada a morfologia do fígado dos fetos. Todos os grupos apresentaram fetos vivos sem alterações macroscópicas, perda pré-implantação e reabsorção fetal normal. Não houve alterações anatômicas nos fígados, apenas presença de monócitos e linfócitos nas veias-centrolobulares. Conclui-se que o extrato, na concentração e formulação estudadas, não apresentou toxicidade aos fetos.(AU)
Although remarkable advances in the use of herbal medicine were observed, studies are needed to demonstrate the toxic potential of their compounds, since most of their users consider them effective and safe. During pregnancy, most of the abnormal changes in the fetus is mainly due to the mother's exposure to chemicals, resulting in abortion or teratogenic and cytotoxic effects to the mother and neonate. The relevance of this research is due to the lack of studies on the toxic effects of Mikania glomerata. popularly known as "guaco" - extract administration during the gestational period. Experimental groups of normotensive and spontaneously hypertensive pregnant Wistar rats were studied. The animals were treated with M. glomerata extract (2 ml) and the morphology of fetal liver was evaluated to evaluate the possible toxic effects of M. glomerata to fetuses. In all groups the live fetuses were without macroscopic changes and the pre-implantation loss or normal fetal reabsorption were physiologically normal (data not shown). There were no anatomical or histological changes in the livers of fetuses studied, which showed centrilobular veins with the presence of monocytes and lymphocytes. The results lead the conclusion that M. glomerata extract, at the tested concentration, did not show toxicity to rats' fetuses.(AU)
Asunto(s)
Animales , Femenino , Embarazo , Desarrollo Fetal , Mikania/toxicidad , Hipertensión , Hígado/patología , Modelos Animales , Fitoterapia/efectos adversos , Ratas WistarRESUMEN
Mikania laevigata (Asteraceae) é popularmente usada no tratamento de doenças respiratórias, incluindo asma, bronquite e tosse. No Brasil, extratos de guaco são usados como produtos medicinais autorizados pela Agência Nacional de Vigilância Sanitária (ANVISA). No estudo, avaliou-se a adaptação e influência da variação sazonal do guaco, em um cultivo mantido em Santarém, Estado do Pará, Brasil; além do monitoramento da cumarina, seu principal constituinte ativo. No cultivo do guaco, as variáveis climáticas e o teor de cumarina foram avaliados mensalmente. A cumarina foi analisada por CCD (Cromatografia em Camada Delgada) e quantificada por HPLC (Cromatografia Liquida de Alta Eficiência) com base nos extratos hidroalcoólico e aquoso das folhas desidratadas. Na estação chuvosa (janeiro a julho), o guaco teve um crescimento homogêneo. Na estação de estiagem (agosto a novembro) houve perda das plantas que cresciam a pleno sol. O teor de cumarina foi detectado durante o desenvolvimento da planta, com níveis que variaram de 0,470 ± 0,01% a 0,886 ± 0,063%. O tempo da coleta não influenciou no teor de cumarina nas plantas cultivadas com 50% de sombra, tendo a média de 0,712% no extrato hidroalcoólico e 0,744% no extrato aquoso. Baseado nos resultados, em condições de sombreamento parcial, assegurou-se que o cultivo do guaco no Baixo Rio Amazonas pode ser replicado pelos pequenos agricultores, em seus Arranjos Produtivos Locais (APL) de plantas medicinais.(AU)
Mikania laevigata (Asteraceae) is popularly used in the treatment of respiratory diseases, including asthma, bronchitis, and cough. In Brazil, guaco extracts are used as medicinal products authorized by the National Health Surveillance Agency (ANVISA).In the study, it was evaluated the adaptation and the influence of the seasonal variation of guaco, in a cultivation held in Santarém, state of Pará, Brazil, and a monitoring of coumarin, its primary active constituent. In the growing of guaco, the climate variables, and the coumarin content were evaluated monthly. The coumarin was analyzed by TLC (Thin Layer Chromatography) and quantified by HPLC (High Performance Liquid Chromatography) from hydroalcoholic and aqueous extracts of dried leaves. In the rainy season (january-july), the guaco had a homogeneous growth. In the dry season (august to november), there was loss of plants grown in full sun. The coumarin content was detected throughout the development of the plant, with levels that varied from 0.470 ± 0.01% to 0.886 ± 0.063%. The time of collection does not influenced in the coumarin content of the plants grown with 50% shade, having the mean of 0.712% in the hydroalcoholic extract and 0.744% in the aqueous extract. Based on the results, in partial shade conditions, it was ensured that small farmers could replicate the cultivation of guaco in the Lower Amazon River, in their local productive arrangements of medicinal plants.(AU)
Asunto(s)
Humanos , Plantas Medicinales/crecimiento & desarrollo , Producción de Cultivos , Cumarinas/análisis , Mikania/crecimiento & desarrollo , Fitoterapia , Brasil , Extractos Vegetales , Ecosistema AmazónicoRESUMEN
Resumo O Estado do Paraná destaca-se pela tradição no cultivo de plantas medicinais. O aumento em sua demanda através da busca de culturas alternativas e rentáveis e o estímulo a uma agricultura ecologicamente sustentável fomentou os agricultores a diversificarem a produção. A pesquisa de campo nos diferentes elos da cadeia produtiva do guaco permitiu construir seu fluxograma, caracterizar o sistema e fluxo de produção, os seus custos e receitas para analisar as Margens e Markups de comercialização. Os sistemas de coleta com enriquecimento e de cultivo apresentaram resultados diferenciados em relação ao seu custo de produção, onde o produtor/coletor alcança uma rentabilidade de 48,9 %, menor margem de comercialização entre todos os elos da cadeia, enquanto o produtor que cultiva o guaco como exploração obtém uma margem de comercialização de 346,4 %. As maiores margens de comercialização (1.733,3 %) estão nos elos fora da porteira das propriedades, percentual entre diferença de preço pago pelo consumidor e o preço recebido pelo produtor/coletor. Os produtores/coletores podem agregar mais renda em vários elos da cadeia produtiva através da organização dos produtores e a melhoria no processo produtivo, possibilitando a melhoria econômica e social nas propriedades familiares, reduzindo o impacto ambiental e garantindo a sustentabilidade dos sistemas de produção.
Abstract The State of Parana stands out for its tradition in the medicinal plants cultivation. The increase of the demand due to the search of alternative and profitable crops and the impulse to an environmentally sustainable agriculture encouraged farmers to diversify production. The field research about the different links of the guaco supply chain allowed the construction of its flowchart, the characterization of the production system and flow, as well as the knowledge of its costs and revenues in order to analyze the commercialization margins and markups. The collection systems with enrichment and the cultivation ones presented different results in relation to its production cost, with the producer/collector reaching a yield of 48.9%, the lowest commercialization margin among all links of the chain, while the farmer who cultivates guaco gets a commercialization margin of 346.4%. The largest commercialization margins (1,733.3%) are in the links outside the properties" gates, as the percentage difference between the price paid by the consumer and the one received by the producer/collector. Producers/collectors can add more income in several links of the supply chain through the producers" organization and by improvements in the production process, enabling the economic and social enhancement at the family farms, thus reducing the environmental impact and ensuring the sustainability of production systems.
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Comercio/estadística & datos numéricos , Productos Agrícolas/clasificación , Mikania/clasificación , Plantas Medicinales/clasificaciónRESUMEN
The objective of this work was to study the molluscicidal activity of aqueous extract of Mikania glomerata on the land snail Subulina octona and to assess the changes caused on the snail's fecundity, growth, hatchability and the offspring produced after exposure. Eggs, newly hatched and 30 day old snails were exposed to the sublethal concentration of M. glomerata extract for 24 and 48 h and the observations were made for 120 days. The presence of tannins, flavonoids and saponins were shown by phytochemical tests. The extract reduced significantly the hatchability and the survival of the offspring hatched from the exposed eggs. The eggs exposed for 48 h presented lower hatchability rates. There was a reduction on survival and growth of newly hatched and 30-days old snails exposed to the extract. These results indicated that the aqueous extract of M. glomerata could be applied as an alternative molluscicide for the control of this snail species efficiently.
RESUMEN
Guaco Mikania glomerata Spreng. and M. laevigata Sch. Bip. ex Baker, Asteraceae, has antimicrobial activity and may be helpful in reducing the incidence of oral diseases. This double-blinded randomized clinical trial aimed to evaluate the efficacy of guaco mouthwashes on the disinfection of toothbrushes used by preschool children, tested positive for mutans streptococci (MS), as well as the quantification of its coumarin contents by high performance liquid chromatography. Ethanol extracts were obtained by percolation. The mouthwashes were prepared with 2.5% g/mL M. glomerata and M. laevigata ethanol extracts, standardized for their coumarin content (% mg/mg). Antimicrobial effect of the mouthwashes and extracts were assessed in vitro against Streptococcus mutans (ATCC 25175TM), using 2.4 to 500 µg/mL to calculate the minimum inhibitory concentration (MIC). For the in vivo study, 24 patients were randomly assigned to a 4-stage changeover system with a one-week interval between each stage. All solutions were used in all stages by a different group of children. After brushing without toothpaste, toothbrushes (n=96) were sprayed with water and solutions of M. glomerata (2.5%), M. laevigata (2.5%) and chlorhexidine (0.12%). Microbiological analysis was carried out after 4 h and 30 days, respectively. MIC values were 400, 125 and 14 µg/mL, respectively, for both crude ethanol extracts, mouthwashes of M. glomerata and M. laevigata. Statistical analysis showed that all solutions decreased contamination of toothbrushes by mutans streptococci (chlorhexidine 50.7±17.7%; M. glomerata 37.3±23.7% and M. laevigata 28.7±25.1% of inhibition). Treatment with chlorhexidine and M. glomerata were statistically similar (p>0.05). M. glomerata mouthwash could be useful in herbal strategy programs against mutans streptococci and the marker coumarin may be not related to the activity observed.
RESUMEN
OBJECTIVES: The Mikania laevigata extract (MLE) (popularly known in Brazil as "guaco") possesses anti-inflammatory properties. In the present study we tested the effects of MLE in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying such effects. MATERIAL AND METHODS: Periodontal disease was induced by a ligature placed around the mandibular first molars of each animal. Male Wistar rats were divided into 4 groups: non-ligated animals treated with vehicle; non-ligated animals treated with MLE (10 mg/kg, daily); ligature-induced animals treated with vehicle and ligature-induced animals treated with MLE (10 mg/kg, daily). Thirty days after the induction of periodontal disease, the animals were euthanized and mandibles and gingival tissues removed for further analysis. RESULTS: Morphometric analysis of alveolar bone loss demonstrated that MLE-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-κB ligand (RANKL) measured by immunohistochemistry. Moreover, gingival tissues from the MLE-treated group showed decreased neutrophil migration myeloperoxidase (MPO) assay. CONCLUSIONS: These results indicate that MLE may be useful to control bone resorption during progression of experimental periodontitis in rats.
Asunto(s)
Animales , Masculino , Ratas , Antiinflamatorios/farmacología , Resorción Ósea/tratamiento farmacológico , Mikania/química , Periodontitis/tratamiento farmacológico , Extractos Vegetales/farmacología , Ligando RANK/efectos de los fármacos , Antiinflamatorios/uso terapéutico , Resorción Ósea/metabolismo , Resorción Ósea/patología , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Hojas de la Planta , Periodontitis/patología , Extractos Vegetales/uso terapéutico , Ligando RANK/metabolismo , Ratas Wistar , Factores de Tiempo , Resultado del TratamientoRESUMEN
As plantas medicinais vêm sendo cada vez mais utilizadas devido às suas propriedades preventivas, paleativas e curativas, além de ser uma terapia alternativa que traz inúmeros benefícios aos usuários. As espécies Mikania glomerata e M. laevigata pertencem à família Asteraceae e são popularmente conhecidas como guaco sendo utilizadas no tratamento de enfermidades do trato respiratório. Além disso, as duas espécies são frequentemente confundidas ou citadas na literatura de forma errada. O presente trabalho teve por objetivo realizar uma revisão bibliográfica sobre a farmacologia, farmacobotânica e fitoquímica dos metabólitos secundários de Guaco, tendo destaque a cumarina, a biossíntese e as ações biológicas. As ações broncodilatadora, expectorante, anti-inflamatória e antialérgica, além de interações com alguns antibióticos e anticoagulantes, também foram descritas neste estudo.
Medicinal plants have been increasingly used due to their preventive, palliative and curative properties, besides being an alternative therapy that brings a large number of benefits to their users. The species Mikania glomerata belongs to the Asteraceae family and is popularly known as guaco, being employed to treat diseases of the respiratory tract. This study aimed to carry out a literature review about the pharmacology, pharmacobotany and phytochemistry of the secondary metabolites of M. glomerata, particularly coumarin, its biosynthesis and biological actions. The bronchodilator, expectorant, anti-inflammatory and antiallergic actions, as well as the interactions with some antibiotics and anticoagulants, were also described in this study.
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Farmacología/métodos , Mikania/química , Fitoterapia , Plantas Medicinales/efectos adversos , Cumarinas/farmacologíaRESUMEN
O objetivo deste trabalho foi avaliar o efeito da temperatura do ar de secagem no rendimento extrativo da cumarina de folhas de guaco. Foram empregados 6 tratamentos de secagem, sendo ar ambiente, ar aquecido a 40, 50, 60, 70 e 80ºC. Utilizou-se secador de bandejas, tendo como fonte de aquecimento o gás liquefeito de petróleo (GLP). Os rendimentos extrativos da cumarina, depois de realizada a secagem, foram comparados com os valores obtidos da planta fresca (tratamento testemunha). A extração da cumarina foi realizada pelo método a quente, em banho-maria a 65ºC, sendo a identificação e quantificação realizada por cromatografia líquida de alta eficiência (CLAE). Em função dos resultados obtidos, pôde-se concluir que a temperatura do ar de secagem a 50ºC possibilitou o melhor resultado para o rendimento extrativo de cumarina em folhas de guaco.
The aim of this study was to evaluate the effect of drying on the extraction yield of coumarin from guaco leaves. Six drying treatments were used, being room air, heated air at 40, 50, 60, 70 and 80ºC. A tray dryer was used with liquefied petroleum gas (LPG) as heating source. The extraction yield of coumarin, after drying, was compared to the values obtained from the fresh plant (control treatment). Coumarin extraction was carried out by using the heat method, in water bath at 65ºC, and identification and quantification were done by means of high performance liquid chromatography (HPLC). Considering the obtained results, the temperature of the drying air at 50ºC led to the best result for the extraction yield of coumarin in guaco leaves.
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Plantas Medicinales/clasificación , Temperatura , Cumarinas/análisis , Hojas de la Planta/clasificación , Mikania/metabolismoRESUMEN
No Brasil as espécies de Mikania glomerata Spreng. e M. laevigata Sch. Bip. ex Baker, Asteraceae, popularmente conhecidas como guaco, são amplamente utilizadas com evidências pré-clínicas para o tratamento de doenças onde estejam envolvidas suas ações broncodilatadoras, antialérgicas, antiasmáticas, anti-inflamatórias, antiulcerogênicas e relaxantes da musculatura lisa. Em virtude das propriedades terapêuticas atribuídas as essas espécies, ambas foram oficializadas na farmacopéia brasileira, e por serem bastante semelhantes, dificilmente são diferenciadas. Com o objetivo de contribuir com novas pesquisas, a presente revisão descreve, de forma sistematizada, sobre os aspectos farmacológicos, toxicológicos, químicos, agronômicos, morfoanatômicos, genéticos e o uso nos programas de fitoterapia do Brasil destas espécies. As principais diferenças e semelhanças entre as duas espécies são evidenciadas através da comparação das informações baseadas na literatura específica.
In Brazil the species of Mikania glomerata Spreng. and M. laevigata Sch. Bip. ex Baker, Asteraceae popularly known as guaco, are widely used with preclinical evidence for the treatment of diseases whose actions involve bronchodilators, antiallergic, anti-asthmatic, anti-inflammatory, antiulcerogenic and smooth muscles relaxant. Due to the therapeutic properties attributed to these species, both were included in the official Brazilian Pharmacopoeia, and because of their similarity, they are hardly differentiated. In order to contribute to the new research, this review describes their several aspects: pharmacology, toxicology, chemical, agricultural, anatomical, genetics and the use in herbal medicine programs in Brazil, in a systematic way. The main differences and similarities between the two species are evidenced by comparing the information based on literature.
RESUMEN
O mercado brasileiro de fitoterápicos encontra-se em crescente expansão, porém a falta de padronização e a má qualidade da matéria-prima vegetal constituem um problema crucial na produção de fitoterápicos de qualidade. Neste trabalho, foram avaliadas drogas vegetais constituídas de folhas (amostras A, B, C) e tinturas (amostras D, E, F) de guaco, adquiridas no Mercado Central e em farmácias de manipulação de Belo Horizonte, respectivamente. Realizaram-se testes físicoquímicos de caracterização organoléptica, determinação de matéria estranha, teores de água e cinzas totais para as amostras de drogas vegetais e caracterização organoléptica, medidas de pH e de densidade para as amostras de tinturas. Foram obtidos os perfis cromatográficos por CCD e CLAE-FR e fez-se a quantificação da cumarina (marcador químico), por CLAE-FR. As amostras analisadas apresentaram um ou mais dos parâmetros avaliados fora dos valores preconizados pela farmacopéia brasileira, evidenciando-se a necessidade da realização do controle de qualidade de matérias-primas vegetais, para se obter medicamentos fitoterápicos de qualidade, eficientes e seguros.
The Brazilian market of herbal medicine is in increasing expansion; however, the lack of standardization and the bad quality of the plant raw materials constitute a crucial problem for the production of quality herbal medicines. In this work, herbal drugs consisting of leaves (samples A, B, C) and tinctures (samples D, E, F) of guaco purchased in the Central Market and from pharmacies in Belo Horizonte, respectively, were evaluated. Physical-chemical tests such as organoleptic characterization, determination of foreign matter, total ash and water content were performed on the samples of herbal drugs. The organoleptic characterization, measures of pH and density of the tincture samples were also determined. TLC and RP-HPLC profiles of the herbal drugs and tinctures were obtained and the quantification of coumarin (used as a marker compound) by RP HPLC was determined. The analyzed samples showed one or more of the evaluated parameters outside of the values recognized by the Brazilian Pharmacopoeia. This demonstrates the need for the quality control of the plant raw materials, in order to guarantee quality and safety in herbal medicine.
RESUMEN
A produção magistral do xarope de guaco, obtido a partir do extrato fluido do guaco (Mikania glomerata Spreng., Asteraceae) e comercializada na Farmácia Universitária da UFJF/MG, gerou um projeto de pesquisa com o objetivo principal de estudar a estabilidade do produto acabado, tendo como ponto de referência a determinação do teor de cumarina das amostras armazenadas em diferentes temperaturas. O método aplicado para realizar a análise do teor de cumarina presente no xarope em estudo foi espectrometria no UV com comprimento de onda de 275,4 nm. Utilizou-se como veículo para efetuar as diluições da amostra uma mistura de metanol/água destilada, na proporção de 80 por cento v/v. A curva de calibração foi obtida diluindo-se 100 mg de cumarina padrão em 100 mL da solução descrito acima, obtendo-se sete concentrações distintas com variação de 2 µg a 20 µg. Os resultados obtidos demonstraram que a temperatura de armazenamento de 45 °C foi considerada ótima para desenvolver a conversão do isômero trans em cis com subseqüente conversão deste a cumarina. Os valores de cumarina encontrados na forma farmacêutica em estudo foram de 1,19 a 1,37 mg/mL, sendo que o valor mais alto refere-se às amostras armazenadas a 45°C durante seis meses.
The production of guaco syrup, obtained from guaco (Mikania glomerata Spreng., Asteraceae) fluid extract, and commercialized by the University Pharmacy of the Federal University of Juiz de Fora-MG, Brazil, led to a research project whose main aim was to study the stability of the finished product, with reference to the coumarin content of samples stored at different temperatures. UV spectrophotometry (275.4 nm) was used to assess the coumarin content of the study syrup. An 80 percent v/v methanol/distilled water mixture was used for sample dilution. The calibration curve was constructed by the dilution of 100 mg standard coumarin in 100 ml of the aforementioned solution, with seven distinct concentrations (ranging from 2 µg a 20 µg) being obtained. The results showed the 45 °C storage temperature to be optimum for the development of trans-cis isomerization, with subsequent conversion of the latter into coumarin. Coumarin content in the studied pharmaceutical presentation ranged from 1.19 to 1.37 mg/mL, the highest value corresponding to the samples stored at 45 °C for six months.
RESUMEN
Adulterações da matéria-prima vegetal ocorrem comumente no mercado de fitoterápicos, sob a forma de substituições e/ou falsificações. Muitas vezes, espécies distintas são comercializadas em substituição à farmacopéica devido à dificuldade de obtenção desta, ou mesmo pelo emprego intencional de espécies de menor valor econômico que apresentem características morfológicas semelhantes. Portanto, faz-se necessária a realização de estudos que busquem identificar a constituição química das espécies de amplo uso medicinal, que são alvos de adulterações. Mikania glomerata e Mikania laevigata, conhecidas popularmente como "guaco", são comercializadas indistintamente. Buscando-se avaliar a composição química das duas espécies, realizou-se uma prospecção fitoquímica por CCD e foram obtidos os perfis cromatográficos por CLAE-FR. Fez-se a quantificação da cumarina empregando-se o mesmo método. Na prospecção fitoquímica por CCD, foi detectada, nas duas espécies, a presença de cumarina, triterpenos/esteróides e heterosídeos flavônicos. Os perfis cromatográficos, obtidos por CLAE-FR, mostraram-se semelhantes, observando-se a predominância de compostos polares. Na quantificação da cumarina, obtêve-se um teor de 0,30 por cento para M. glomerata e 0,43 por cento para M. laevigata, teores de acordo com o preconizado na monografia do guaco-cheiroso (mín. 0,1 por cento). M glomerata e M. laevigata apresentaram composição química semelhante e teores de cumarina próximos, sugerindo que estas podem ser utilizadas de forma indistinta.
Adulterations of plant raw materials often occur in the marketing of herbal medicine, usually in the form of substitutions and/ or fakes. Many times, different plant species are commercialized in the place of the one recognized by the pharmacopoeia. This may occur due to the difficulties of obtaining the authentic plant material, or by an intentional use of a plant species that has less economic value but shows similar morphologic features. Therefore, it is necessary to carry out studies that can identify the chemical composition of those species of wide medicinal use, which are the targets of adulterations. Mikania glomerata and Mikania laevigata, popularly known as "guaco", are indistinctly commercialized in Brazil. A phytochemical screening by TLC was carried out to evaluate the chemical composition of both species. The RP-HPLC profiles were obtained and the quantification of coumarin was determined by the same method. A phytochemical screening has shown that coumarin, triterpenes/ steroids and flavonic heterosides are the constituents detected in both species. The RP-HPLC profiles were similar between the two species and revealed the predominance of polar compounds. In the quantification of coumarin, a content of 0.30 percent (w/w) was obtained for M. glomerata and 0.43 percent (w/w) for M. laevigata. These values are in accord with the contents (min. 0.1 percent, w/w) described by the monograph of "guaco-cheiroso" in the Brazilian Pharmacopoeia. Therefore, the results indicated similar chemical profiles for M. glomerata and M. laevigata, as well as comparable coumarin contents, thus suggesting that the species might be used indistinctly.
RESUMEN
Mikania glomerata Spreng. (Asteracae) is a plant widely used to treat gastrointestinal disorders in Brazilian traditional medicine. In the present work, an aqueous extract of the leaves of Mikania glomerata has shown a decrease in the propulsive movements of the intestinal contents in mice. Oral administration produced an inhibition of gastrointestinal transit as effective as that produced by loperamide. These findings suggested that the aqueous extract of the leaves of Mikania glomerata might elicit an antidiarrhoeal effect by inhibiting intestinal motility.