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The depletion of nutrient sources in fertilizers demands a paradigm shift in the treatment of nutrient-rich wastewater, such as urine, to enable efficient resource recovery and high-value conversion. This study presented an integrated bipolar membrane electrodialysis (BMED) and hollow fiber membrane (HFM) system for near-complete resource recovery and zero-discharge from urine treatment. Computational simulations and experimental validations demonstrated that a higher voltage (20 V) significantly enhanced energy utilization, while an optimal flow rate of 0.4 L/min effectively mitigated the negative effects of concentration polarization and electro-osmosis on system performance. Within 40 min, the process separated 90.13% of the salts in urine, with an energy consumption of only 8.45 kWh/kgbase. Utilizing a multi-chamber structure for selective separation, the system achieved recovery efficiencies of 89% for nitrogen, 96% for phosphorus, and 95% for potassium from fresh urine, converting them into high-value products such as 85 mM acid, 69.5 mM base, and liquid fertilizer. According to techno-economic analysis, the cost of treating urine using this system at the lab-scale was $6.29/kg of products (including acid, base, and (NH4)2SO4), which was significantly lower than the $20.44/kg cost for the precipitation method to produce struvite. Excluding fixed costs, a net profit of $18.24/m3 was achieved through the recovery of valuable products from urine using this system. The pilot-scale assessment showed that the net benefit amounts to $19.90/m3 of urine, demonstrating significant economic feasibility. This study presents an effective approach for the near-complete resource recovery and zero-discharge treatment of urine, offering a practical solution for sustainable nutrient recycling and wastewater management.
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The electrochemical propylene epoxidation reaction (PER) provides a promising route for ecofriendly propylene oxide (PO) production, instantly generating active halogen/oxygen species to alleviate chloride contamination inherent in traditional PER. However, the complex processes and unsatisfactory PO yield for current electrochemical PER falls short of meeting industrial application requirements. Herein, a spatial-coupling strategy over RuO2/Ti hollow-fiber penetration electrode (HPE) is adopted to facilitate efficient PO production, significantly improving PER performance to the ampere level (achieving over 80% PO faradaic efficiency and a maximum PO current density of 859 mA cm-2). The synergetic combination of the penetration effect of HPE and the spatial-coupled reaction sequence, enables the realization of ampere-level PO production with high specificity, exhibiting significant potentials for economically viable PER applications.
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Tobacco flavors are extensively utilized in traditional tobacco products, electronic nicotine, heated tobacco products, and snuff. To inhibit fungal growth arising from high moisture content, preservatives such as benzoic acid (BA), sorbic acid (SA), and parabens are often incorporated into tobacco flavors. Nonetheless, consuming preservatives beyond safety thresholds may pose health risks. Therefore, analytical determination of these preservatives is crucial for both quality assurance and consumer protection. For example, BA and SA can induce adverse reactions in susceptible individuals, including asthma, urticaria, metabolic acidosis, and convulsions. Parabens, because of their endocrine activity, are classified as endocrine-disrupting chemicals. Despite extensive research, the concurrent quantification of trace-level hydrophilic (BA and SA) and hydrophobic (methylparaben, ethylparaben, isopropylparaben, propylparaben, butylparaben, isobutylparaben, and benzylparaben) preservatives in tobacco flavors remains challenging. Traditional liquid phase extraction coupled with high performance liquid chromatography (HPLC) often results in high false positive rates and inadequate sensitivity. In contrast, tandem mass spectrometry offers high sensitivity and specificity; however, its widespread application is limited by laborious sample preparation and significant operational costs. Therefore, it is crucial to establish a fast and sensitive sample pretreatment and analysis method for the nine preservatives in tobacco flavors. In this study, a method for the simultaneous determination of the nine preservatives (SA, BA and seven parabens) in tobacco flavor was established based on three phase-hollow fiber-liquid phase microextraction (3P-HF-LPME) technology combined with HPLC. To obtain the optimal pretreatment conditions, extraction solvent type, sample phase pH, acceptor phase pH, sample phase volume, extraction time, and mass fraction of sodium chloride, were examined. Additionally, the HPLC parameters, including UV detection wavelength and mobile phase composition, were refined. The optimal extraction conditions were as follows: dihexyl ether was used as extraction solvent, 15 mL sample solution (pH 4) was used as sample phase, sodium hydroxide aqueous solution (pH 12) was used as acceptor phase, and the extraction was carried out at 800 r/min for 30 min. Chromatographic separation was accomplished using an Agilent Poroshell 120 EC-C18 column (100 mm×3 mm, 2.7 µm) and a mobile phase comprising methanol, 0.02 mol/L ammonium acetate aqueous solution (containing 0.5% acetic acid), and acetonitrile for gradient elution. Under the optimized conditions, the nine target analytes showed good linear relationships in their respective linear ranges, the correlation coefficients (r) were ≥0.9967, limits of detection (LODs) and quantification (LOQs) were 0.02-0.07 mg/kg and 0.08-0.24 mg/kg, respectively. Under two spiked levels, the enrichment factors (EFs) and extraction recoveries (ERs) of the nine target analytes were 30.6-91.1 and 6.1%-18.2%, respectively. The recoveries of the nine target analytes ranged from 82.2% to 115.7% and the relative standard deviations (RSDs) (n=5) were less than 14.5% at low, medium and high levels. The developed method is straightforward, precise, sensitive, and well-suited for the rapid screening of preservatives in tobacco flavor samples.
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Microextracción en Fase Líquida , Parabenos , Conservadores Farmacéuticos , Cromatografía Líquida de Alta Presión , Parabenos/análisis , Microextracción en Fase Líquida/métodos , Conservadores Farmacéuticos/análisis , Ácido Benzoico/análisis , Nicotiana/química , Ácido Sórbico/análisis , Aromatizantes/análisis , Productos de Tabaco/análisisRESUMEN
Since the COVID-19 pandemic of 2020-2023, extracorporeal membrane oxygenator (ECMO) has attracted considerable attention worldwide. It is expected that ECMO with long-term durability is put into practical use in order to prepare for next emerging infectious diseases and to facilitate manufacturing for novel medical devices. Polypropylene (PP) and polymethylpentene (PMP) capillary membranes are currently the mainstream for gas exchange membrane for ECMO. ECMO support days for COVID-19-related acute hypoxemic respiratory failure have been reported to be on average for 14 or 24 days. It is necessary to improve opposing functions such that promoting the permeation of oxygen and carbon dioxide and inhibiting the permeation of water vapor or plasma to develop sufficient durability for long-term use. For this purpose, accurately controlling the anisotropy of the pore structure of the entire cross section and functions of capillary membrane is significant. In this study, we focused on the cross-sectional ion-milling (CSIM) method, to precisely clarify the pore structure of the entire cross section of capillary membrane for ECMO, because there is less physical stress on the porous structure applied during the preparation of cross-sectional samples of porous capillary membranes. We attempted to observe the cross sections of commercially available PMP membranes using the CSIM method. As a result, we succeeded in fabricating fine-scale flat cross-sectional samples of PMP capillary membranes. The pore structures and the degree of anisotropy of the cross sections are quantitatively clarified. The achievements and the approaches of this study are being applied to the development of next-generation gas exchange membranes.
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Therapeutic drug monitoring (TDM) of imatinib (IM) in cancer therapy offers the potential to improve treatment efficacy while minimizing toxicity. There was a significant correlation between unbound concentration and clinical response and toxicity, compared with total plasma concentrations, and the quantification of unbound IM and its metabolite, N-desmethyl imatinib (NDI) are of interest for TDM. However, traditional unbound drug separation methods have shortcomings, especially are susceptible to non-specific binding (NSB) of drugs to the polymer-constructed components of filter membranes, which are difficult to avoid at present. Hence it is necessary to developed a reliable separation method for the analysis of the unbound fraction of IM and NDI in TDM. We developed and validated an hollow fiber solid phase microextraction (HF-SPME) method coupled with high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) that to measure unbound IM and NDI concentration in human plasma. It used the NSB phenomenon and solve the NSB problem. The preparation procedure only involves a common vortex and ultrasonication without dilution of samples and modification of membrane. A total of 50 chronic myeloid leukemia (CML) patients were enrolled in our study. The relationship between the unbound and total concentrations for IM and NDI, as well as the concentration ratios of NDI to IM in 50 clinical plasma samples were investigated. The extraction recovery is high to 95.5-106â¯% with validation parameters for the methodological results were all excellent. There were both a poor linear relationship between the unbound and total concentrations for IM (r2=0.504) and NDI (r2=0.201) in 50 clinical plasma samples. The unbound concentration ratios of NDI to IM varied widely in CML patients. The determination of unbound IM and NDI concentration is meaningful and necessary. The developed HF-SPME method is simple, accurate and precise that could be used to measure unbound IM and NDI concentration in clinical TDM.
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Traditional peroxymonosulfate (PMS) catalytic membranes dominated by radical pathways often face interference from complex components in water bodies. Herein, we employed a controlled electro-deposition technique to coat a Ni-Co metal-organic framework (MOF) precursor onto titanium hollow fiber membrane (THFM), followed by high-temperature calcination to synthesize a MOF-derived NiO-NiCo2O4/THFM (M-NNCO-THFM) PMS catalytic membrane. Then, the M-NNCO-THFM filtration integrated with PMS activation (MFPA process) for water treatment. Experimental results demonstrated that the M-NNCO-THFM MFPA process successfully achieved complete phenol (PE) removal via a non-radical-dominated degradation pathway, involving singlet oxygen (1O2) and electron transfer, while exhibiting wide pH adaptability and exceptional stability in complex water matrices. Mechanism analysis revealed that the electron transfer process was significantly enhanced by the MOF-derived heterojunction structure, which increased the flat-band potential from 0.39 eV to 0.56 eV, thereby facilitating efficient electron transfer for PE removal. The non-radical 1O2 pathway was primarily due to the cycling of metal valence states (Ni2+/Co3+), leading to the reduction of Co2+ and its reaction with PMS, resulting in the generation of reactive species. Furthermore, electrochemical measurements indicated that the M-NNCO-THFM exhibited lower charge transfer resistance and enhanced charge transfer efficiency compared to non-MOF-derived NNCO-THFM, corresponding to the superior catalytic performance and electrochemically active surface area of M-NNCO-THFM.
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Hollow-fiber membranes for nanofiltration were prepared from the blending of Poly (ethylene glycol) (PEG) with Poly (vinyl chloride) (PVC) with different PEG molecular weights (400 and 4000 g/mol) and PVC via a dry/wet spinning process. In the spinning process, the effects of air gap, wind-up speed, dope extrusion rate, and bore extrusion rate were examined. In addition, the different lengths of the center tube, which acted as the inner-side fiber diameter during the preparation of hollow-fiber membranes, were studied. This research was investigated in order to observe the morphological, dielectric, and dynamic mechanical thermal properties to identify a suitable preparation of a hollow-fiber membrane for feasible applications. The morphology of the PVC-580 blended PEG-400 5 weight percent hollow-fiber membrane was seen to have a dense skin on both the inner and outer fiber surface, along with a suitable dope viscosity. Moreover, it offered finger-like substructures that could provide a high applicable feed-stream permeability and selectivity. Finger-like substructures were present on the near inner fiber surface at the controlled center-tube length of 0.3 cm, more so than at the center tube of 1 cm. This was because the solvent and non-solvent in the lumen tube exchanged more quickly than they did in the coagulant bath. The effect of the wind-up speed during the spinning process was significantly influenced by an affordable hollow fiber that can be indicated by the drawing ratio (λ). It was found that the drawing ratio of 3.3 showed a thickness thinner than 2.6 and 2.0, respectively. In summary, a controlled wind-up speed, an acceptable dope viscosity, and-most importantly-an agglomerated time resulted in membrane preparation.
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Carbon dioxide (CO2) can be converted to valuable organic chemicals using light irradiation and photocatalysis. Today, light-energy loss, poor conversion efficiency, and low quantum efficiency (QE) hamper the application of photocatalytic CO2 reduction. To overcome these drawbacks, we developed an efficient photocatalytic reactor platform for producing formic acid (HCOOH) by coating an iron-based metal-organic framework (Fe-MOF) onto side-emitting polymeric optical fibers (POFs) and using hollow-fiber membranes (HFMs) to deliver bubble-free CO2. The photocatalyst, Fe-MOF with amine-group (-NH2) decoration, provided exceptional dissolved inorganic carbon (DIC) absorption. The dual-fiber system gave a CO2-to-HCOOH conversion rate of 116 ± 1.2 mM h-1 g-1, which is ≥18-fold higher than the rates in photocatalytic slurry systems. The 12% QE obtained using the POF was 18-fold greater than the QE obtained by a photocatalytic slurry. The conversion efficiency and product selectivity of CO2-to-HCOOH were up to 22 and 99%, respectively. Due to the dual efficiencies of bubble-free CO2 delivery and the high QE achieved using the POF platform, the dual-fiber system had energy consumption of only 0.60 ± 0.05 kWh mol-1, 3000-fold better than photocatalysis using slurry-based systems. This innovative dual-fiber design enables efficient CO2 valorization without the use of platinum group metals or rare earth elements.
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Dióxido de Carbono , Dióxido de Carbono/química , Catálisis , Fibras ÓpticasRESUMEN
The use of animal models is still widespread in science but there is a movement away from this manner of experimentation. One option approved by the FDA for human-like studies is the hollow fiber bioreactor (HFS). HFSs are highly controllable, self-contained systems that allow for the modeling of individual tissues and disease phenotypes. Oxygen, drug concentration & half-life, and immune cell invasion are all scalable to human and veterinary conditions using a HFS. There are drawbacks to the systems including cost and contamination so the use of these systems must be carefully managed.With these limitations in mind, the scope of the technology is great. Antimicrobial susceptibility testing (AST) is possible with greater accuracy and clinical validity than classical in vitro techniques making minimal inhibitory concentration (MIC) data generated on the bench more translatable to the clinic.In this chapter, we will outline the background of the HFS and some typical uses.
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Reactores Biológicos , Pruebas de Sensibilidad Microbiana , Humanos , Pruebas de Sensibilidad Microbiana/métodos , Animales , Antibacterianos/farmacología , Farmacorresistencia Bacteriana/efectos de los fármacosRESUMEN
Introduced here is the on-line coupling of hollow-fiber flow field-flow fractionation (HF5) to depolarized multi-angle static light scattering (D-MALS). HF5 is a size-based separation alternative to size-exclusion and hydrodynamic chromatography and asymmetric flow field-flow fractionation. HF5 can separate larger sizes than its chromatographic counterparts and provides several advantages over its fractionation counterpart, including reduced sample consumption and greater ease of operation. D-MALS is a variant of MALS in which the depolarized scattering from the analyte solution is measured at a variety of angles simultaneously. Measurements of depolarized scattering have previously been employed in studying the optical properties of solutions or suspensions, to determine the length of rod-like analytes, and to gain increased accuracy in the determination of analyte molar mass. The coupling HF5/D-MALS allows for the depolarization ratio of a solution or suspension to be measured continuously across the fractogram. This is demonstrated here for a Teflon latex the size range of which extends beyond that accessible to commercial size-exclusion columns. The results presented provide the first reported on-line HF5/D-MALS coupling, showing the feasibility of the technique as well as its realized potential for providing continuous depolarization measurements, inter alia.
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Fraccionamiento de Campo-Flujo , Luz , Dispersión de Radiación , Fraccionamiento de Campo-Flujo/métodos , Tamaño de la PartículaRESUMEN
This work presents methods of obtaining polymeric hollow-fiber membranes produced via the dry-wet phase inversion method that were published in renowned specialized membrane publications in the years 2010-2020. Obtaining hollow-fiber membranes, unlike flat membranes, requires the use of a special installation for their production, the most important component of which is the hollow fiber forming spinneret. This method is most often used in obtaining membranes made of polysulfone, polyethersulfone, polyurethane, cellulose acetate, and its derivatives. Many factors affect the properties of the membranes obtained. By changing the parameters of the spinning process, we change the thickness of the membranes' walls and the diameter of the hollow fibers, which causes changes in the membranes' structure and, as a consequence, changes in their transport/separation parameters. The type of bore fluid affects the porosity of the inner epidermal layer or causes its atrophy. Porogenic compounds such as polyvinylpyrrolidones and polyethylene glycols and other substances that additionally increase the membrane porosity are often added to the polymer solution. Another example is a blend of two- or multi-component membranes and dual-layer membranes that are obtained using a three-nozzle spinneret. In dual-layer membranes, one layer is the membrane scaffolding, and the other is the separation layer. Also, the temperature during the process, the humidity, and the composition of the solution in the coagulating bath have impact on the parameters of the membranes obtained.
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Chondrocyte-based cell therapy has been used for more than 30 years and is still considered to be a promising method of cartilage repair despite some limitations. This review introduces the latest developments of four generations of autologous chondrocyte implantation and current autologous chondrocyte products. The regeneration of cartilage from adult chondrocytes is limited by culture-induced dedifferentiation and patient age. Cartibeads is an innovative three-step method to produce high-quality hyaline cartilage microtissues, and it is developed from adult dedifferentiated chondrocytes with a high number of cell passages. In addition, allogeneic chondrocyte therapies using the Quantum hollow-fiber bioreactor and several signaling pathways involved in chondrocyte-based cartilage repair are mentioned, such as WNT signaling, the BMP-2/WISP1 pathway, and the FGF19 pathway.
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The separation layer prepared by the conventional coating-crosslinking method is typically thick and prone to forming defective macropores, significantly affecting the water permeability and dye/salt separation performance of membranes. This work presented a novel method to prepare hollow fiber composite membranes for dye/salt separation based on the opposite transmission reaction of crosslinker. In this method, the macromolecule in situ reacted with a small-molecule crosslinker at the openings of membrane pore channels, forming a separation layer with discontinuous sheet-like and granular structure. Compared to the conventional forward coating-crosslinking method, the separation layer prepared by the opposite transmission reaction method exhibited an ultra-thin thickness of 29.1 nm. Consequently, the composite membrane exhibited a high water permeability of 72.7 L·m-2·h-1·bar-1, which was 2.3 times higher than that of conventional methods. Moreover, the prepared composite membrane presented a more uniformed pore structure, completely retaining the VBB (100%) with a low Na2SO4 rejection of 4.3%, demonstrating excellent dye/salt separation performance. Additionally, the prepared composite membrane exhibited superior anti-fouling properties compared to that prepared by the conventional method. Therefore, the opposite transmission reaction method proposed in this study held promising applications in the preparation of hollow fiber composite membranes for efficient dye/salt separation.
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As an important part of the membrane field, hollow fiber membranes (HFM) have been widely concerned by scholars. HFM fouling in the industrial application results in a reduction in its lifespan and an increase in cost. In recent years, various explorations on the HFM fouling control strategies have been carried out. In the current work, we critically review the influence of flow field characteristics in HFM-based bioreactor on membrane fouling control. The flow field characteristics mainly refer to the spatial and temporal variation of the related physical parameters. In the HFM field, the physical parameter mainly refers to the variation characteristics of the shear force, flow velocity and turbulence caused by hydraulics. The factors affecting the flow field characteristics will be discussed from three levels: the micro-flow field near the interface of membrane (micro-interface), the flow field around the membrane module and the reactor design related to flow field, which involves surface morphology, crossflow, aeration, fiber packing density, membrane vibration, structural design and other related parameters. The study of flow field characteristics and influencing factors in the HFM separation process will help to improve the performance of HFM in full-scale water treatment plants.
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Reactores Biológicos , Membranas Artificiales , Purificación del Agua/métodos , Purificación del Agua/instrumentaciónRESUMEN
Separating helium (He) and hydrogen (H2), two gases that are extremely similar in molecular size and condensation properties, presents a formidable challenge in the helium industry. The development of membranes capable of precisely differentiating between these gases is crucial for achieving large-scale, energy-efficient He/H2 separation. However, the limited selectivity of current membranes has hindered their practical application. In this study, we propose a novel approach to overcome this challenge by engineering submicroporous membranes through the fluorination of partially carbonized hollow fibers. We demonstrate that the fluorine substitution on the inner rim of the micropore walls within the carbon hollow fibers enables tunability of the microporous architecture. Furthermore, it enhances interactions between H2 molecules and the micropore walls through the polarization and hydrogen bonding induced by C-F bonds, resulting in simultaneous improvements in both He/H2 diffusivity and solubility selectivities. The fluorinated HFM-550-F-1â min membrane exhibits exceptional mixed-gas separation performance, with a binary mixed-gas He/H2 selectivity of 10.5 and a ternary mixed-gas He/(H2+CO2) selectivity of 20.8, at 40â bar feed pressure and 35 °C, surpassing all previously reported polymer-based gas separation membranes, and remarkable plasticization resistance and long-term continuous stability over 30â days.
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Covalent organic frameworks (COFs), known for their chemical stability and porous crystalline structure, hold promises as advanced separation membranes. However, fabricating high-quality COF membranes, particularly on industrial-preferred hollow fiber substrates, remains challenging. This study introduces a novel vapor/vapor-solid (V/V-S) method for growing ultrathin crystalline TpPa-1 COF membranes on the inner lumen surface of alumina hollow fibers (TpPa-1/Alumina). Through vapor-phase monomer introduction onto polydopamine-modified alumina at 170 °C and 1â atm, efficient polymerization and crystallization occur at the confined V-S interface. This enables one-step growth within 8â h, producing 100â nm thick COF membranes with strong substrate adhesion. TpPa-1/Alumina exhibits exceptional stability and performance over 80â h in continuous cross-flow organic solvent nanofiltration (OSN), with methanol permeance of about 200â L m-2 h-1 bar-1 and dye rejection with molecular weight cutoff (MWCO) of approximately 700â Da. Moreover, the versatile V/V-S method synthesizes two additional COF membranes (TpPa2Cl/Alumina and TpHz/Alumina) with different pore sizes and chemical environments. Adjusting the COF membrane thickness between 100-500â nm is achievable easily by varying the growth cycle numbers. Notably, TpPa2Cl/Alumina demonstrates excellent OSN performance in separating the model active pharmaceutical ingredient glycyrrhizic acid (GA) from dimethyl sulfoxide (DMSO), highlighting the method's potential for large-scale industrial applications.
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In the current study, a wide range of deep eutectic solvents (DESs) with different properties (hydrophilic, hydrophobic, ionic, and nonionic) were prepared in the initial phase. Subsequently, an assessment was conducted to evaluate some characteristics of the produced DESs, including their stability at room temperature and their capacity to extract three distinct types of analytes (anionic, cationic, and non-ionic) simultaneously through hollow fiber-liquid phase microextraction (HF-LPME) technique. To carry out the extraction procedure, the prepared DESs were inserted into the pores (as supported liquid membrane (SLM)) and lumen of hollow fiber membrane (HF) to apply two-phase and three-phase HF-LPME techniques. After a thorough evaluation, the three-phase HF-LPME technique (HF(3)-LPME) was chosen by using a mixture of menthol/TBAB-based hydrophobic DES (DES-35) as SLM and the mixture of malic acid/citric acid/water-based hydrophilic DES (DES-2) as an extraction solvent in the lumen of HF. All factors affecting the extraction recovery (including pH, extraction time, extraction temperature, stirring speed, and salt effect) were optimized utilizing the one-variable-at-a-time (OVAT) methodology. After applying the extraction procedure, all extracted samples were analyzed using the UV-Vis spectrometer and results were recorded at different wavelengths including 655 nm for Methylene blue, 550 nm for Amaranth, and 375 nm for Quercetin. The calibration graphs showed linearity in the range of 20.0-1500 µg/L, with a limit of detection of 6.2-15.1 µg/L and correlation coefficients higher than 0.9913 for the studied analytes. Moreover, the intra-day RSD, inter-day RSD, preconcentration factor (PF), enrichment factors (EF), and extraction recoveries (ER%) were obtained in the range of 3.1-4.8, 3.8-6.7, 125, 102.9-111.4, and 82.3-89.1 %, respectively. The use of the selected DES in the HF-LPME methodology resulted in an ecologically friendly strategy, as evidenced by the use of green metrics from the SPMS tool. The proposed strategy is also considered environmentally friendly due to its use of minimal solvents, waste reduction, and low energy consumption. The proposed technique effectively and simultaneously extractedmethylene blue, amaranth, and quercetin analytes in different real samples.
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Single cell protein (SCP, or microbial protein) is one of the emerging alternative protein sources to address the global challenge of food insecurity. Recently, the SCP produced from methane has attracted substantial attention since methane is a renewable resource attainable from anaerobic digestion. However, the supply of methane, an insoluble gas in water, is one of the major challenges in producing methane-based SCP. This work developed a novel bioreactor configuration, in which hollow fiber membrane was used for efficient methane supply while microorganisms were growing in the suspended form favourable for the biomass harvest. Over a 312-day operation, the impacts of three critical parameters on the SCP production were investigated, including the ratio of methane loading to ammonium loading, the ratio of methane loading to oxygen loading, and the sludge retention time (SRT). Under the condition of 4 g CH4/g NH4+, 4 g O2/g CH4, and SRT of 4 days, the highest SCP production yield was observed and determined to be 1.36 g SCP/g CH4 and 5.05 g SCP/g N, respectively. The protein content was up to 67 %, which is higher than the majority of reported values to date. Moreover, the methane and ammonium utilization efficiencies were both close to 100 %, suggesting the highly efficient utilization of substrates in this new bioreactor configuration. A high relative abundance of essential amino acids (EAA) above 42 % was achieved, representing the highest EAA content reported. These findings provide valuable insights into SCP production using methane as a feedstock.
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Reactores Biológicos , Metano , Metano/metabolismo , Proteínas Bacterianas/metabolismo , Compuestos de Amonio/metabolismo , Membranas Artificiales , Aguas del Alcantarillado , Proteínas en la DietaRESUMEN
Based on the specific binding of drug molecules to cell membrane receptors, a screening and separation method for active compounds of natural products was established by combining phospholipase C (PLC) sensitized hollow fiber microscreening by a solvent seal with high-performance liquid chromatography technology. In the process, the factors affecting the screening were optimized. Under the optimal screening conditions, we screened honokiol (HK), magnolol (MG), negative control drug carbamazepine, and positive control drug amentoflavone, the repeatability of the method was tested. The PLC activity was determined before and after the screening. Experimental results showed that the sensitization factors of PLC of HK and MG were 61.0 and 48.5, respectively, and amentoflavone was 15.0, carbamazepine could not bind to PLC. Moreover, the molecular docking results were consistent with this measurement, indicating that HK and MG could be combined with PLC, and they were potential interacting components with PLC. This method used organic solvent to seal the PLC greatly ensuring the activity, so this method had the advantage of integrating separation, and purification with screening, it not only exhibited good reproducibility and high sensitivity but was also suitable for screening the active components in natural products by various targets in vitro.